CN102978274A - Method for applying anaerobic dry fermentation technology to treating rice straws to prepare biological flocculating agent - Google Patents
Method for applying anaerobic dry fermentation technology to treating rice straws to prepare biological flocculating agent Download PDFInfo
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- CN102978274A CN102978274A CN201210523161XA CN201210523161A CN102978274A CN 102978274 A CN102978274 A CN 102978274A CN 201210523161X A CN201210523161X A CN 201210523161XA CN 201210523161 A CN201210523161 A CN 201210523161A CN 102978274 A CN102978274 A CN 102978274A
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- producing bacteria
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Abstract
The invention provides a method for applying an anaerobic dry fermentation technology to treating rice straws to prepare a biological flocculating agent, and relates to the resource utilization of agricultural wastes. The method comprises the following steps of: carrying out anaerobic fermentation on the rice straws and collecting acid-producing floras of a normal gas-producing methane tank to carry out enrichment and domestication; starting and operating an anaerobic reactor by using an inoculating amount of 30% at 30 DEG C; continuously operating for 80-120 days; after taking a fermentation solution in the reactor to centrifuge for 10 minutes, sterilizing at a high temperature and under high pressure; mixing with the conventional flocculation producing culture medium in a volume ratio of 1:1; and inoculating 8% of flocculation producing bacteria to obtain the biological flocculating agent. According to the method, a rice straw fermentation solution can be can efficiently utilized to prepare the biological flocculating agent so as to realize the aim of preparing the biological flocculating agent by using cheap substrates; and the method has a wide application prospect, can be used for large-scale production application of the biological flocculating agent, and provides important technical references for high-value energy conversion of the wastes.
Description
Technical field
The present invention relates to the agricultural wastes recycling, it is a kind of method that anaerobic dry fermentation technical finesse rice straw is produced biological flocculant of using, it is take rice straw as substrate, using the anaerobic dry fermentation technology degrades, utilize acidogenic fermentation liquid to produce biological flocculant, the development and use cheap substrates is produced the new technology of biological flocculant.
Background technology
Biological flocculant is the polymkeric substance that a class has biological degradation function, comprises polysaccharide, lipid and protein etc. by the Institute of Micro-biology secretion with this class specific function.Because therefore its safety and nonstaining property to environment are subject to the attention of lot of domestic and foreign researcher.At present, be widely used in the removal research of all kinds of organic waste waters of processing and some feature chemical substances, metallic element.Owing to produce the more needed substrates of biological flocculant, such as high costs such as sucrose, glucose and fructose, cause being difficult to mass-producing application and industrialization.Yet former studies person study to concentrate on and separate efficiently, have the microorganism of producing the flocculation agent function, and optimization is cultivated the domestication condition and resolved the research of its flocculation mechanism and chemical structure.Having ignored the alternative materials of seeking novel cheapness also is effectively to solve one of effective way of using this difficult problem as the substrate of producing biological flocculant.
In recent years, there are some to substitute the report that traditional substratum are produced flocculation agent about utilizing other cheapnesss to substitute substrates, as utilize crop material, organic waste water and brewing wastewater etc. to substitute carbon source as substrate and produce flocculation agent.But using anaerobic fermentation process, to give birth to methanogenic fermented liquid and natural pond liquid be that the research that substrate is produced biological flocculant yet there are no report.Produce the research of flocculation agent for the application anaerobic fermented liquid and can not only deepen the yardstick of waste ' s reclamation, and avoid natural pond liquid to cause secondary environmental pollution by efficient recovery, also open up new technological approaches for the utilization of agricultural wastes comprehensive resource simultaneously.Make simultaneously the suitable High-efficient Production biological flocculant of natural pond liquid physicochemical property that reference is provided for efficiently producing methane from technique regulation and control fermentation system in the future.
Summary of the invention
The objective of the invention is to produce biological flocculant for using anaerobic dry fermentation technical finesse rice straw.Characteristics of the present invention are to use the anaerobic dry fermentation technology, take rice straw as substrate, concentration carries out the acidogenic fermentation degraded as 20%, realized producing with cheap substrates the new way of biological flocculant.
The present invention implements like this: a kind ofly use the method that anaerobic dry fermentation technical finesse rice straw is produced biological flocculant, it is characterized in that step is:
1) rice straw is treated to 3 ~ 5 centimetres of sections, take initial dry substance concentration (TS) as 20% anaerobically fermenting;
2) pass into first more than high pure nitrogen 15 min in the anti-oxygen device of anaerobism, the dissolved oxygen of driving away in the anaerobic reactor makes the interior redox potential (ORP) of system be lower than-250 mV;
3) the acid-producing bacteria group who gathers the normal gas producing methane-generating pit carries out acclimating, and 30 ℃ of startup operations of the inoculum size with 30% anaerobic reactor moves 80-120 days continuously;
4) get more than interior centrifugal 10 min of fermented liquid of anaerobic reactor, produce the substratum of wadding a quilt with cotton with tradition behind the autoclave sterilization and mix the acquisition complex medium with volume ratio 1:1, described tradition is produced among the wadding substratum 1L and is comprised: glucose 10 g, yeast extract 0.5 g, urea 0.5 g, K2HPO4 5 g, KH2PO4 2 g, MgSO4 0.2 g, NaCl 0.1 g, adjusting pH7.0 ~ 7.2;
5) in complex medium, obtain biological flocculant behind the inoculation flcos producing bacteria F+, described flcos producing bacteria F+ is the flcos producing bacteria F2(Rhizobium radiobacter that separates with Heilongjiang Province Environmental Biotechnology key lab) and F6(Bacillussphaericus) equal-volume mix the compound flcos producing bacteria F+ that forms than 1:1, inoculum size is 8%.
In present method, adopt continuously 100 days fermented liquid of operation in the complex medium, can obtain best product wadding effect.
The degraded acid-producing bacteria group that the present invention utilizes is natural pond liquid in the semicontinuous fermentation methane-generating pit of steady running, flcos producing bacteria is the compound flcos producing bacteria F+ that F2+F6 forms with volume ratio 1:1 balanced mix, this method can efficiently utilize the rice straw fermented liquid to produce biological flocculant, realized that cheap substrates produces biological flocculant, have broad application prospects, be the production application of in the future extensive biological flocculant, realize that the high value energy conversion of waste provides the important technology reference.
Description of drawings
PH changing conditions in Fig. 1 rice straw anaerobic dry fermentation process.
VFA changing conditions in Fig. 2 rice straw anaerobic dry fermentation process.
Fig. 3 different times 1:1 complex medium flocculation efficiency and reducing sugar total amount are relatively.
Fig. 4 produces sour peak period (doing the 25th day fermented liquid of fermentation operation) fermented liquid superior microorganism kind.
Embodiment
A kind ofly use the method that anaerobic dry fermentation technical finesse rice straw is produced biological flocculant, step is:
1) rice straw is treated to 3 ~ 5 centimetres of sections, take initial dry substance concentration as 20% anaerobically fermenting;
2) pass into first more than high pure nitrogen 15 min in the anti-oxygen device of anaerobism, the dissolved oxygen of driving away in the anaerobic reactor makes the interior redox potential (ORP) of system be lower than-250 mV;
3) the acid-producing bacteria group who gathers the normal gas producing methane-generating pit carries out acclimating, and 30 ℃ of startup operations of the inoculum size with 30% anaerobic reactor moves 80-120 days continuously;
4) get more than interior 100 days centrifugal 10 min of fermented liquid 9000 g of reactor, produce the substratum of wadding a quilt with cotton with tradition behind the autoclave sterilization and mix the acquisition complex medium with volume ratio 1:1, described tradition is produced among the wadding substratum 1L and is comprised: glucose 10 g, yeast extract 0.5 g, urea 0.5 g, K2HPO4 5 g, KH2PO4 2 g, MgSO4 0.2 g, NaCl 0.1 g, adjusting pH7.0 ~ 7.2;
5) in complex medium, obtain biological flocculant behind the inoculation flcos producing bacteria F+, described flcos producing bacteria F+ is the flcos producing bacteria F2(Rhizobium radiobacter that separates with Heilongjiang Province Environmental Biotechnology key lab) and F6(Bacillussphaericus) equal-volume mix the compound flcos producing bacteria F+ that forms than 1:1, inoculum size is 8%.
Compound flcos producing bacteria F+ with each in period acidogenic fermentation liquid with produce the complex medium that the traditional substratum of wadding forms and mix, measure flocculation efficiency through 24 h, complex medium flocculating rate the best that 100 days shown in Figure 4 fermented liquids form reaches 92.45%.
Monitor the fermented liquid Comparison of Microbial Community of pH, VFA (VFA) and produce flocculant in the anaerobic fermentation process, referring to Fig. 1, Fig. 2, Fig. 3.
Use the order-checking of 454 tetra-sodium flux and carry out the bacterial flora monitoring, Fig. 4 is for producing sour peak period, the anaerobic ferment devices operation fermented liquid bacterial flora diversity in the time of 25 days, on the categorization levels of door take Firmicutes (Firmicutes) as the main advantage microbe groups.
Claims (2)
1. use the method that anaerobic dry fermentation technical finesse rice straw is produced biological flocculant for one kind, it is characterized in that step is:
1) rice straw is treated to 3 ~ 5 centimetres of sections, take initial dry substance concentration as 20% anaerobically fermenting;
2) pass into first more than high pure nitrogen 15 min in the anti-oxygen device of anaerobism, the dissolved oxygen of driving away in the anaerobic reactor makes the interior redox potential of system be lower than-250 mV;
3) the acid-producing bacteria group who gathers the normal gas producing methane-generating pit carries out acclimating, and 30 ℃ of startup operations of the inoculum size with 30% anaerobic reactor moves 80-120 days continuously;
4) get more than interior centrifugal 10 min of fermented liquid of anaerobic reactor, produce the wadding substratum with tradition behind the autoclave sterilization and mix the acquisition complex medium with volume ratio 1:1, described tradition is produced among the wadding substratum 1L and is comprised: glucose 10 g, yeast extract 0.5 g, urea 0.5 g, K
2HPO
45 g, KH
2PO
42 g, MgSO
40.2 g, NaCl 0.1 g, adjusting pH7.0 ~ 7.2;
5) inoculation flcos producing bacteria F in complex medium
+Rear acquisition biological flocculant, described flcos producing bacteria F
+The flcos producing bacteria F2(that separates with Heilongjiang Province Environmental Biotechnology key lab
Rhizobium radiobacter) and F6(
Bacillussphaericus) the compound flcos producing bacteria F that mix to form than 1:1 of equal-volume
+, inoculum size is 8%.
2. application anaerobic dry fermentation technical finesse rice straw according to claim 1 is produced the method for biological flocculant, it is characterized in that adopting in the complex medium continuously 100 days fermented liquid of operation, obtains best product wadding effect.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105217805A (en) * | 2015-11-09 | 2016-01-06 | 成都信息工程大学 | A kind of microbial flocculant, preparation method and application thereof |
| CN105671087A (en) * | 2016-04-25 | 2016-06-15 | 江苏强农农业技术服务有限公司 | Method for preparing microbial flocculant from biological wastes |
| CN106119144A (en) * | 2016-04-11 | 2016-11-16 | 江苏师范大学 | A kind of Pericarppium arachidis hypogaeae hydrolysate that converts prepares method and the bacterial strain of microbial flocculant |
| CN114381474A (en) * | 2022-01-11 | 2022-04-22 | 武汉纺织大学 | Degradable biological flocculant and preparation method and preparation device thereof |
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| DE102007054150A1 (en) * | 2007-11-12 | 2009-05-14 | Bayer Technology Services Gmbh | Process for the production of biobutanol from biomass |
| CN102199630A (en) * | 2011-04-15 | 2011-09-28 | 无锡丰陆环保科技有限公司 | Anaerobic dry fermentation method for generating biogas and used system thereof |
| CN102260729A (en) * | 2011-06-24 | 2011-11-30 | 哈尔滨工业大学 | Bioflocculant fermentation method with mycelium pellet as vector |
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2012
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102007054150A1 (en) * | 2007-11-12 | 2009-05-14 | Bayer Technology Services Gmbh | Process for the production of biobutanol from biomass |
| CN102199630A (en) * | 2011-04-15 | 2011-09-28 | 无锡丰陆环保科技有限公司 | Anaerobic dry fermentation method for generating biogas and used system thereof |
| CN102260729A (en) * | 2011-06-24 | 2011-11-30 | 哈尔滨工业大学 | Bioflocculant fermentation method with mycelium pellet as vector |
Non-Patent Citations (1)
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| 王哲: "水稻秸秆不同工艺条件下发酵液作为底物的产絮效能研究", 《中国优秀硕士学位论文全文数据库，工程科技Ⅰ辑》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105217805A (en) * | 2015-11-09 | 2016-01-06 | 成都信息工程大学 | A kind of microbial flocculant, preparation method and application thereof |
| CN106119144A (en) * | 2016-04-11 | 2016-11-16 | 江苏师范大学 | A kind of Pericarppium arachidis hypogaeae hydrolysate that converts prepares method and the bacterial strain of microbial flocculant |
| CN106119144B (en) * | 2016-04-11 | 2019-07-26 | 江苏师范大学 | A kind of conversion peanut shell hydrolysate prepares the method and bacterial strain of microbial flocculant |
| CN105671087A (en) * | 2016-04-25 | 2016-06-15 | 江苏强农农业技术服务有限公司 | Method for preparing microbial flocculant from biological wastes |
| CN114381474A (en) * | 2022-01-11 | 2022-04-22 | 武汉纺织大学 | Degradable biological flocculant and preparation method and preparation device thereof |
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Application publication date: 20130320 |