CN114381474A - Degradable biological flocculant and preparation method and preparation device thereof - Google Patents
Degradable biological flocculant and preparation method and preparation device thereof Download PDFInfo
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Abstract
The invention belongs to the field of preparation of biological flocculants, and particularly relates to a degradable biological flocculant, which is prepared from the following materials: 50-60 parts of straw fermentation liquor, 30-40 parts of glucose, 0.5-1.5 parts of magnesium sulfate, 1-2 parts of monopotassium phosphate, 20-30 parts of agar, 5-15 parts of gel and efficient flocculant producing bacteria. The invention provides energy for the strains by using the straw fermentation liquor to replace part of glucose, thereby saving the production cost of the flocculant, consuming part of straw and protecting the environment.
Description
Technical Field
The invention belongs to the field of preparation of biological flocculants, and particularly relates to a degradable biological flocculant and a preparation method and a preparation device thereof.
Background
Compared with the chemical flocculating agent, the microbial flocculating agent is a macromolecular organic matter with a flocculation function generated by microorganisms, and comprises polysaccharide and protein as main components, so that the microbial flocculating agent is easy to biodegrade, has small environmental hazard and no secondary pollution, is simple to prepare, low in cost and short in production period, and is a novel environment-friendly wastewater treating agent.
Biological flocculation agent among the prior art need use a large amount of glucose to provide the energy for the bacterial at the in-process of production to make manufacturing cost promote, and when cultivateing the bacterial, the inside of unable assurance incubator is heated evenly and is not convenient for take out the culture medium. Accordingly, there is a need for improvements in the art.
Disclosure of Invention
The invention aims to provide a degradable biological flocculant, a preparation method and a preparation device thereof, which solve the problems of higher production cost and nonuniform heating inside an incubator of the conventional biological flocculant.
In order to achieve the purpose, the invention provides a degradable biological flocculant, which comprises the following materials in parts by weight: 50-60 parts of straw fermentation liquor, 30-40 parts of glucose, 0.5-1.5 parts of magnesium sulfate, 1-2 parts of monopotassium phosphate, 20-30 parts of agar, 5-15 parts of gel and efficient flocculant producing bacteria.
A preparation method of a degradable biological flocculant comprises the following steps:
firstly, preparing specified parts of straw fermentation liquor, glucose, high-efficiency flocculant producing bacteria, magnesium sulfate, monopotassium phosphate, agar, gel and high-efficiency flocculant producing bacteria.
And step two, placing the straw fermentation liquor into a high-temperature sterilization tank for high-temperature sterilization treatment, and cooling the straw fermentation liquor after sterilizing the sundry bacteria in the straw fermentation liquor.
And step three, putting the cooled straw fermentation broth, glucose, magnesium sulfate, potassium dihydrogen phosphate and agar into a mixing device, and fully mixing to obtain the culture medium.
And step four, inoculating the efficient flocculant producing bacteria into the culture medium, and then putting the culture medium into an incubator for constant-temperature culture.
And step five, after the cultured high-efficiency flocculant culture solution is prepared, putting the culture solution into a centrifuge, and separating the high-efficiency flocculant in the centrifuge, so that the preparation of the high-efficiency flocculant can be finished.
Further, the straw fermentation liquid in the first step can be made of one or more of wheat straw, corn straw or rice straw.
Further, the temperature of the high-temperature sterilization tube in the second step is 130 ℃, and the sterilization time is 15 minutes.
Further, the temperature of the incubator in the fourth step is 40 ℃, and the incubation time is two days.
Further, the temperature of the centrifuge in the fifth step is 10 ℃.
A preparation device of a degradable bioflocculant comprises a box body, wherein a bearing is fixedly sleeved in the lower end of the box body, a rotating shaft is fixedly sleeved in the bearing, a plurality of uniformly distributed fixing boxes are fixedly connected to the outer side of the rotating shaft, four uniformly distributed square rods are slidably connected in each fixing box, a spring is arranged on the outer side of each square rod, one end of each square rod is fixedly connected with a clamping block, three uniformly distributed constant temperature heaters are fixedly connected to the inner side of the box body, the inner sides of the three constant temperature heaters are fixedly connected with a heat conduction cover, a plurality of uniformly distributed heat conduction plates are fixedly connected to the inner side of the heat conduction cover, a driving box is fixedly connected to the lower end of the box body, a first bevel gear is fixedly connected to one end of the rotating shaft, and a speed reduction motor is fixedly connected to the inner part of the driving box, the tail end of an output shaft of the speed reducing motor is fixedly connected with a bevel gear II, the outer side of the box body is hinged with a box door, and the bevel gear I is meshed with the bevel gear II.
Further, the one end of spring with clamping piece fixed connection, the other end of spring with fixed box fixed connection, the other end fixedly connected with limiting plate of square pole, the limiting plate with fixed box contact.
Furthermore, the lower extreme fixedly connected with four supporting legs of drive case, four the supporting leg is in the lower extreme evenly distributed of drive case.
The method has the advantages that the straw fermentation liquor is used for replacing a part of glucose to provide energy for the strains, so that the production cost of the flocculant can be saved, a part of straws can be consumed, and the environment can be protected.
Through add in the inside of box and establish pivot, fixed box and heat conduction cover isotructure, can drive the inside culture dish of fixed box through the pivot and rotate when cultivateing the bacterial to can let the bacterial slowly rotate in the inside of heat conduction cover, make the bacterial be heated evenly.
Drawings
FIG. 1 is a schematic diagram of a device for preparing a degradable bioflocculant according to an embodiment of the present invention;
FIG. 2 is a top view of FIG. 1 of a device for preparing a degradable bioflocculant according to an embodiment of the invention;
FIG. 3 is a rear view of FIG. 1 of a device for preparing a degradable bioflocculant according to an embodiment of the invention;
fig. 4 is an enlarged view of a portion a of the structure of fig. 2 of the apparatus for preparing a degradable bioflocculant according to the embodiment of the present invention.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: the box body 1, the bearing 2, the rotating shaft 3, the fixed box 4, the square rod 5, the spring 6, the clamping block 7, the limiting plate 8, the constant temperature heater 9, the heat conducting cover 10, the heat conducting plate 11, the first bevel gear 12, the speed reducing motor 13, the second bevel gear 14, the driving box 15, the supporting legs 16 and the box door 17.
Example 1:
the degradable biological flocculant comprises the following materials in parts by weight: 50g of straw fermentation liquor, 30g of glucose, 0.5g of magnesium sulfate, 1g of monopotassium phosphate, 20g of agar, 5g of gel and efficient flocculant producing bacteria.
A preparation method of a degradable biological flocculant comprises the following steps:
firstly, preparing specified parts of straw fermentation liquor, glucose, high-efficiency flocculant producing bacteria, magnesium sulfate, monopotassium phosphate, agar, gel and high-efficiency flocculant producing bacteria.
And step two, placing the straw fermentation liquor into a high-temperature sterilization tank for high-temperature sterilization treatment, and cooling the straw fermentation liquor after sterilizing the sundry bacteria in the straw fermentation liquor.
And step three, putting the cooled straw fermentation broth, glucose, magnesium sulfate, potassium dihydrogen phosphate and agar into a mixing device, and fully mixing to obtain the culture medium.
And step four, inoculating the efficient flocculant producing bacteria into the culture medium, and then putting the culture medium into an incubator for constant-temperature culture.
And step five, after the cultured high-efficiency flocculant culture solution is prepared, putting the culture solution into a centrifuge, and separating the high-efficiency flocculant in the centrifuge, so that the preparation of the high-efficiency flocculant can be finished.
The straw fermentation liquor in the first step can be made of one or more of wheat straw, corn straw or rice straw.
And the temperature of the high-temperature sterilization tube in the second step is 130 ℃, and the sterilization time is 15 minutes.
The temperature of the incubator in the fourth step is 40 ℃, and the incubation time is two days.
And the temperature of the centrifuge in the fifth step is 10 ℃.
Referring to fig. 1-4, a device for preparing a degradable bioflocculant comprises a box body 1, a bearing 2 is fixedly sleeved inside the lower end of the box body 1, a rotating shaft 3 is fixedly sleeved inside the bearing 2, a plurality of uniformly distributed fixing boxes 4 are fixedly connected to the outer side of the rotating shaft 3, four uniformly distributed square rods 5 are slidably connected inside each fixing box 4, a spring 6 is arranged outside each square rod 5, a clamping block 7 is fixedly connected to one end of each square rod 5, three uniformly distributed constant temperature heaters 9 are fixedly connected inside the box body 1, a heat conduction cover 10 is fixedly connected to the inner sides of the three constant temperature heaters 9, a plurality of uniformly distributed heat conduction plates 11 are fixedly connected inside the heat conduction cover 10, a driving box 15 is fixedly connected to the lower end of the box body 1, a bevel gear I12 is fixedly connected to one end of the rotating shaft 3, and a speed reduction motor 13 is fixedly connected inside the driving box 15, the tail end of an output shaft of the speed reducing motor 13 is fixedly connected with a second bevel gear 14, the outer side of the box body 1 is hinged with a box door 17, the first bevel gear 12 is meshed with the second bevel gear 14, and the heat conducting plates 11 can uniformly dissipate heat to the inside of the box body 1.
Referring to fig. 1, 2 and 4, one end of the spring 6 is fixedly connected to the clamping block 7, the other end of the spring 6 is fixedly connected to the fixing box 4, the other end of the square rod 5 is fixedly connected to the limiting plate 8, the limiting plate 8 contacts with the fixing box 4, and the spring 6 can push the clamping block 7 to fix the culture dish inside the box body 1 through elastic force.
Referring to fig. 1, four support legs 16 are fixedly connected to the lower end of the driving box 15, the four support legs 16 are uniformly distributed at the lower end of the driving box 15, and the support legs 16 support the driving box 15.
The specific implementation process of the invention is as follows: when using, promote clamping block 7, make spring 6 compressed, then put into the inside round hole of fixed box 4 with the culture dish inside, then loosen clamping block 7, spring 6 resets, spring 6 promotes clamping block 7 through elasticity and fixes the culture dish in the inside of fixed box 4, then start constant temperature heater 9, constant temperature heater 9 transmits the inside of heat-conducting plate 11 through heat conduction cover 10 with the heat, the inside of heat-conducting plate 11 is dispersed to the heat of inside to box 1 that heat-conducting plate 11 is even, when constant temperature heater 9 heats the inside of box 1, start gear motor 13, gear motor 13 drives bevel gear 12 through bevel gear two 14 and rotates, bevel gear two 12 drives fixed box 4 through pivot 3 and rotates, fixed box 4 drives the pivoted in-process of culture dish, can make being heated of culture dish more even.
Example 2:
the degradable biological flocculant comprises the following materials in parts by weight: 55g of straw fermentation liquid, 35g of glucose, 1g of magnesium sulfate, 1.5g of monopotassium phosphate, 20g of agar, 10g of gel and efficient flocculant producing bacteria.
A preparation method of a degradable biological flocculant comprises the following steps:
firstly, preparing specified parts of straw fermentation liquor, glucose, high-efficiency flocculant producing bacteria, magnesium sulfate, monopotassium phosphate, agar, gel and high-efficiency flocculant producing bacteria.
And step two, placing the straw fermentation liquor into a high-temperature sterilization tank for high-temperature sterilization treatment, and cooling the straw fermentation liquor after sterilizing the sundry bacteria in the straw fermentation liquor.
And step three, putting the cooled straw fermentation broth, glucose, magnesium sulfate, potassium dihydrogen phosphate and agar into a mixing device, and fully mixing to obtain the culture medium.
And step four, inoculating the efficient flocculant producing bacteria into the culture medium, and then putting the culture medium into an incubator for constant-temperature culture.
And step five, after the cultured high-efficiency flocculant culture solution is prepared, putting the culture solution into a centrifuge, and separating the high-efficiency flocculant in the centrifuge, so that the preparation of the high-efficiency flocculant can be finished.
The straw fermentation liquor in the first step can be made of one or more of wheat straw, corn straw or rice straw.
And the temperature of the high-temperature sterilization tube in the second step is 130 ℃, and the sterilization time is 15 minutes.
The temperature of the incubator in the fourth step is 40 ℃, and the incubation time is two days.
And the temperature of the centrifuge in the fifth step is 10 ℃.
Referring to fig. 1-4, a device for preparing a degradable bioflocculant comprises a box body 1, a bearing 2 is fixedly sleeved inside the lower end of the box body 1, a rotating shaft 3 is fixedly sleeved inside the bearing 2, a plurality of uniformly distributed fixing boxes 4 are fixedly connected to the outer side of the rotating shaft 3, four uniformly distributed square rods 5 are slidably connected inside each fixing box 4, a spring 6 is arranged outside each square rod 5, a clamping block 7 is fixedly connected to one end of each square rod 5, three uniformly distributed constant temperature heaters 9 are fixedly connected inside the box body 1, a heat conduction cover 10 is fixedly connected to the inner sides of the three constant temperature heaters 9, a plurality of uniformly distributed heat conduction plates 11 are fixedly connected inside the heat conduction cover 10, a driving box 15 is fixedly connected to the lower end of the box body 1, a bevel gear I12 is fixedly connected to one end of the rotating shaft 3, and a speed reduction motor 13 is fixedly connected inside the driving box 15, the tail end of an output shaft of the speed reducing motor 13 is fixedly connected with a second bevel gear 14, the outer side of the box body 1 is hinged with a box door 17, the first bevel gear 12 is meshed with the second bevel gear 14, and the heat conducting plates 11 can uniformly dissipate heat to the inside of the box body 1.
Referring to fig. 1, 2 and 4, one end of the spring 6 is fixedly connected to the clamping block 7, the other end of the spring 6 is fixedly connected to the fixing box 4, the other end of the square rod 5 is fixedly connected to the limiting plate 8, the limiting plate 8 contacts with the fixing box 4, and the spring 6 can push the clamping block 7 to fix the culture dish inside the box body 1 through elastic force.
Referring to fig. 1, four support legs 16 are fixedly connected to the lower end of the driving box 15, the four support legs 16 are uniformly distributed at the lower end of the driving box 15, and the support legs 16 support the driving box 15.
The specific implementation process of the invention is as follows: when using, promote clamping block 7, make spring 6 compressed, then put into the inside round hole of fixed box 4 with the culture dish inside, then loosen clamping block 7, spring 6 resets, spring 6 promotes clamping block 7 through elasticity and fixes the culture dish in the inside of fixed box 4, then start constant temperature heater 9, constant temperature heater 9 transmits the inside of heat-conducting plate 11 through heat conduction cover 10 with the heat, the inside of heat-conducting plate 11 is dispersed to the heat of inside to box 1 that heat-conducting plate 11 is even, when constant temperature heater 9 heats the inside of box 1, start gear motor 13, gear motor 13 drives bevel gear 12 through bevel gear two 14 and rotates, bevel gear two 12 drives fixed box 4 through pivot 3 and rotates, fixed box 4 drives the pivoted in-process of culture dish, can make being heated of culture dish more even.
Example 3:
the degradable biological flocculant comprises the following materials in parts by weight: 60g of straw fermentation liquor, 40g of glucose, 1.5g of magnesium sulfate, 2g of monopotassium phosphate, 30g of agar, 15g of gel and high-efficiency flocculant producing bacteria.
A preparation method of a degradable biological flocculant comprises the following steps:
firstly, preparing specified parts of straw fermentation liquor, glucose, high-efficiency flocculant producing bacteria, magnesium sulfate, monopotassium phosphate, agar, gel and high-efficiency flocculant producing bacteria.
And step two, placing the straw fermentation liquor into a high-temperature sterilization tank for high-temperature sterilization treatment, and cooling the straw fermentation liquor after sterilizing the sundry bacteria in the straw fermentation liquor.
And step three, putting the cooled straw fermentation broth, glucose, magnesium sulfate, potassium dihydrogen phosphate and agar into a mixing device, and fully mixing to obtain the culture medium.
And step four, inoculating the efficient flocculant producing bacteria into the culture medium, and then putting the culture medium into an incubator for constant-temperature culture.
And step five, after the cultured high-efficiency flocculant culture solution is prepared, putting the culture solution into a centrifuge, and separating the high-efficiency flocculant in the centrifuge, so that the preparation of the high-efficiency flocculant can be finished.
The straw fermentation liquor in the first step can be made of one or more of wheat straw, corn straw or rice straw.
And the temperature of the high-temperature sterilization tube in the second step is 130 ℃, and the sterilization time is 15 minutes.
The temperature of the incubator in the fourth step is 40 ℃, and the incubation time is two days.
And the temperature of the centrifuge in the fifth step is 10 ℃.
Referring to fig. 1-4, a device for preparing a degradable bioflocculant comprises a box body 1, a bearing 2 is fixedly sleeved inside the lower end of the box body 1, a rotating shaft 3 is fixedly sleeved inside the bearing 2, a plurality of uniformly distributed fixing boxes 4 are fixedly connected to the outer side of the rotating shaft 3, four uniformly distributed square rods 5 are slidably connected inside each fixing box 4, a spring 6 is arranged outside each square rod 5, a clamping block 7 is fixedly connected to one end of each square rod 5, three uniformly distributed constant temperature heaters 9 are fixedly connected inside the box body 1, a heat conduction cover 10 is fixedly connected to the inner sides of the three constant temperature heaters 9, a plurality of uniformly distributed heat conduction plates 11 are fixedly connected inside the heat conduction cover 10, a driving box 15 is fixedly connected to the lower end of the box body 1, a bevel gear I12 is fixedly connected to one end of the rotating shaft 3, and a speed reduction motor 13 is fixedly connected inside the driving box 15, the tail end of an output shaft of the speed reducing motor 13 is fixedly connected with a second bevel gear 14, the outer side of the box body 1 is hinged with a box door 17, the first bevel gear 12 is meshed with the second bevel gear 14, and the heat conducting plates 11 can uniformly dissipate heat to the inside of the box body 1.
Referring to fig. 1, 2 and 4, one end of the spring 6 is fixedly connected to the clamping block 7, the other end of the spring 6 is fixedly connected to the fixing box 4, the other end of the square rod 5 is fixedly connected to the limiting plate 8, the limiting plate 8 contacts with the fixing box 4, and the spring 6 can push the clamping block 7 to fix the culture dish inside the box body 1 through elastic force.
Referring to fig. 1, four support legs 16 are fixedly connected to the lower end of the driving box 15, the four support legs 16 are uniformly distributed at the lower end of the driving box 15, and the support legs 16 support the driving box 15.
The specific implementation process of the invention is as follows: when using, promote clamping block 7, make spring 6 compressed, then put into the inside round hole of fixed box 4 with the culture dish inside, then loosen clamping block 7, spring 6 resets, spring 6 promotes clamping block 7 through elasticity and fixes the culture dish in the inside of fixed box 4, then start constant temperature heater 9, constant temperature heater 9 transmits the inside of heat-conducting plate 11 through heat conduction cover 10 with the heat, the inside of heat-conducting plate 11 is dispersed to the heat of inside to box 1 that heat-conducting plate 11 is even, when constant temperature heater 9 heats the inside of box 1, start gear motor 13, gear motor 13 drives bevel gear 12 through bevel gear two 14 and rotates, bevel gear two 12 drives fixed box 4 through pivot 3 and rotates, fixed box 4 drives the pivoted in-process of culture dish, can make being heated of culture dish more even.
Comparative example 1:
the degradable biological flocculant comprises the following materials in parts by weight: 30g of glucose, 0.5g of magnesium sulfate, 1g of monopotassium phosphate, 20g of agar and high-efficiency flocculant producing bacteria.
A preparation method of a degradable biological flocculant comprises the following steps:
step one, preparing specified parts of glucose, high-efficiency flocculant producing bacteria, magnesium sulfate, monopotassium phosphate, agar and high-efficiency flocculant producing bacteria.
And step two, placing the straw fermentation liquor into a high-temperature sterilization tank for high-temperature sterilization treatment, and cooling the straw fermentation liquor after sterilizing the sundry bacteria in the straw fermentation liquor.
And step three, putting the cooled straw fermentation broth, glucose, magnesium sulfate, potassium dihydrogen phosphate and agar into a mixing device, and fully mixing to obtain the culture medium.
And step four, inoculating the efficient flocculant producing bacteria into the culture medium, and then putting the culture medium into an incubator for constant-temperature culture.
And step five, after the cultured high-efficiency flocculant culture solution is prepared, putting the culture solution into a centrifuge, and separating the high-efficiency flocculant in the centrifuge, so that the preparation of the high-efficiency flocculant can be finished.
The straw fermentation liquor in the first step can be made of one or more of wheat straw, corn straw or rice straw.
And the temperature of the high-temperature sterilization tube in the second step is 130 ℃, and the sterilization time is 15 minutes.
The temperature of the incubator in the fourth step is 40 ℃, and the incubation time is two days.
And the temperature of the centrifuge in the fifth step is 10 ℃.
Please refer to fig. 1-4, a device for preparing a degradable bioflocculant, comprising a box body 1, a bearing 2 fixedly sleeved inside the lower end of the box body 1, a rotating shaft 3 fixedly sleeved inside the bearing 2, a plurality of uniformly distributed fixing boxes 4 fixedly connected outside the rotating shaft 3, four uniformly distributed square rods 5 slidably connected inside each fixing box 4, a spring 6 arranged outside each square rod 5, a clamping block 7 fixedly connected to one end of each square rod 5, three uniformly distributed constant temperature heaters 9 fixedly connected inside the box body 1, a heat conduction cover 10 fixedly connected to the inner side of each constant temperature heater 9, a plurality of uniformly distributed heat conduction plates 11 fixedly connected inside the heat conduction cover 10, a driving box 15 fixedly connected to the lower end of the box body 1, a bevel gear 12 fixedly connected to one end of the rotating shaft 3, and a speed reduction motor 13 fixedly connected inside the driving box 15, the tail end of an output shaft of the speed reducing motor 13 is fixedly connected with a second bevel gear 14, the outer side of the box body 1 is hinged with a box door 17, the first bevel gear 12 is meshed with the second bevel gear 14, and the heat conducting plates 11 can uniformly dissipate heat to the inside of the box body 1.
Referring to fig. 1, 2 and 4, one end of the spring 6 is fixedly connected to the clamping block 7, the other end of the spring 6 is fixedly connected to the fixing box 4, the other end of the square rod 5 is fixedly connected to the limiting plate 8, the limiting plate 8 contacts with the fixing box 4, and the spring 6 can push the clamping block 7 to fix the culture dish inside the box body 1 through elastic force.
Referring to fig. 1, four support legs 16 are fixedly connected to the lower end of the driving box 15, the four support legs 16 are uniformly distributed at the lower end of the driving box 15, and the support legs 16 support the driving box 15.
The specific implementation process of the invention is as follows: when using, promote clamping block 7, make spring 6 compressed, then put into the inside round hole of fixed box 4 with the culture dish inside, then loosen clamping block 7, spring 6 resets, spring 6 promotes clamping block 7 through elasticity and fixes the culture dish in the inside of fixed box 4, then start constant temperature heater 9, constant temperature heater 9 transmits the inside of heat-conducting plate 11 through heat conduction cover 10 with the heat, the inside of heat-conducting plate 11 is dispersed to the heat of inside to box 1 that heat-conducting plate 11 is even, when constant temperature heater 9 heats the inside of box 1, start gear motor 13, gear motor 13 drives bevel gear 12 through bevel gear two 14 and rotates, bevel gear two 12 drives fixed box 4 through pivot 3 and rotates, fixed box 4 drives the pivoted in-process of culture dish, can make being heated of culture dish more even.
Comparative example 2:
the degradable biological flocculant comprises the following materials in parts by weight: 35g of glucose, 1g of magnesium sulfate, 1.5g of monopotassium phosphate, 25g of agar and high-efficiency flocculant producing bacteria.
A preparation method of a degradable biological flocculant comprises the following steps:
step one, preparing specified parts of glucose, high-efficiency flocculant producing bacteria, magnesium sulfate, monopotassium phosphate, agar and high-efficiency flocculant producing bacteria.
And step two, placing the straw fermentation liquor into a high-temperature sterilization tank for high-temperature sterilization treatment, and cooling the straw fermentation liquor after sterilizing the sundry bacteria in the straw fermentation liquor.
And step three, putting the cooled straw fermentation broth, glucose, magnesium sulfate, potassium dihydrogen phosphate and agar into a mixing device, and fully mixing to obtain the culture medium.
And step four, inoculating the efficient flocculant producing bacteria into the culture medium, and then putting the culture medium into an incubator for constant-temperature culture.
And step five, after the cultured high-efficiency flocculant culture solution is prepared, putting the culture solution into a centrifuge, and separating the high-efficiency flocculant in the centrifuge, so that the preparation of the high-efficiency flocculant can be finished.
The straw fermentation liquor in the first step can be made of one or more of wheat straw, corn straw or rice straw.
And the temperature of the high-temperature sterilization tube in the second step is 130 ℃, and the sterilization time is 15 minutes.
The temperature of the incubator in the fourth step is 40 ℃, and the incubation time is two days.
And the temperature of the centrifuge in the fifth step is 10 ℃.
Referring to fig. 1-4, a device for preparing a degradable bioflocculant comprises a box body 1, a bearing 2 is fixedly sleeved inside the lower end of the box body 1, a rotating shaft 3 is fixedly sleeved inside the bearing 2, a plurality of uniformly distributed fixing boxes 4 are fixedly connected to the outer side of the rotating shaft 3, four uniformly distributed square rods 5 are slidably connected inside each fixing box 4, a spring 6 is arranged outside each square rod 5, a clamping block 7 is fixedly connected to one end of each square rod 5, three uniformly distributed constant temperature heaters 9 are fixedly connected inside the box body 1, a heat conduction cover 10 is fixedly connected to the inner sides of the three constant temperature heaters 9, a plurality of uniformly distributed heat conduction plates 11 are fixedly connected inside the heat conduction cover 10, a driving box 15 is fixedly connected to the lower end of the box body 1, a bevel gear I12 is fixedly connected to one end of the rotating shaft 3, and a speed reduction motor 13 is fixedly connected inside the driving box 15, the tail end of an output shaft of the speed reducing motor 13 is fixedly connected with a second bevel gear 14, the outer side of the box body 1 is hinged with a box door 17, the first bevel gear 12 is meshed with the second bevel gear 14, and the heat conducting plates 11 can uniformly dissipate heat to the inside of the box body 1.
Referring to fig. 1, 2 and 4, one end of the spring 6 is fixedly connected to the clamping block 7, the other end of the spring 6 is fixedly connected to the fixing box 4, the other end of the square rod 5 is fixedly connected to the limiting plate 8, the limiting plate 8 contacts with the fixing box 4, and the spring 6 can push the clamping block 7 to fix the culture dish inside the box body 1 through elastic force.
Referring to fig. 1, four support legs 16 are fixedly connected to the lower end of the driving box 15, the four support legs 16 are uniformly distributed at the lower end of the driving box 15, and the support legs 16 support the driving box 15.
The specific implementation process of the invention is as follows: when using, promote clamping block 7, make spring 6 compressed, then put into the inside round hole of fixed box 4 with the culture dish inside, then loosen clamping block 7, spring 6 resets, spring 6 promotes clamping block 7 through elasticity and fixes the culture dish in the inside of fixed box 4, then start constant temperature heater 9, constant temperature heater 9 transmits the inside of heat-conducting plate 11 through heat conduction cover 10 with the heat, the inside of heat-conducting plate 11 is dispersed to the heat of inside to box 1 that heat-conducting plate 11 is even, when constant temperature heater 9 heats the inside of box 1, start gear motor 13, gear motor 13 drives bevel gear 12 through bevel gear two 14 and rotates, bevel gear two 12 drives fixed box 4 through pivot 3 and rotates, fixed box 4 drives the pivoted in-process of culture dish, can make being heated of culture dish more even.
Comparative example 3:
the degradable biological flocculant comprises the following materials in parts by weight: 40g of glucose, 1.5g of magnesium sulfate, 2g of monopotassium phosphate, 30g of agar and high-efficiency flocculant producing bacteria.
A preparation method of a degradable biological flocculant comprises the following steps:
step one, preparing specified parts of glucose, high-efficiency flocculant producing bacteria, magnesium sulfate, monopotassium phosphate, agar and high-efficiency flocculant producing bacteria.
And step two, placing the straw fermentation liquor into a high-temperature sterilization tank for high-temperature sterilization treatment, and cooling the straw fermentation liquor after sterilizing the sundry bacteria in the straw fermentation liquor.
And step three, putting the cooled straw fermentation broth, glucose, magnesium sulfate, potassium dihydrogen phosphate and agar into a mixing device, and fully mixing to obtain the culture medium.
And step four, inoculating the efficient flocculant producing bacteria into the culture medium, and then putting the culture medium into an incubator for constant-temperature culture.
And step five, after the cultured high-efficiency flocculant culture solution is prepared, putting the culture solution into a centrifuge, and separating the high-efficiency flocculant in the centrifuge, so that the preparation of the high-efficiency flocculant can be finished.
The straw fermentation liquor in the first step can be made of one or more of wheat straw, corn straw or rice straw.
And the temperature of the high-temperature sterilization tube in the second step is 130 ℃, and the sterilization time is 15 minutes.
The temperature of the incubator in the fourth step is 40 ℃, and the incubation time is two days.
And the temperature of the centrifuge in the fifth step is 10 ℃.
Referring to fig. 1-4, a device for preparing a degradable bioflocculant comprises a box body 1, a bearing 2 is fixedly sleeved inside the lower end of the box body 1, a rotating shaft 3 is fixedly sleeved inside the bearing 2, a plurality of uniformly distributed fixing boxes 4 are fixedly connected to the outer side of the rotating shaft 3, four uniformly distributed square rods 5 are slidably connected inside each fixing box 4, a spring 6 is arranged outside each square rod 5, a clamping block 7 is fixedly connected to one end of each square rod 5, three uniformly distributed constant temperature heaters 9 are fixedly connected inside the box body 1, a heat conduction cover 10 is fixedly connected to the inner sides of the three constant temperature heaters 9, a plurality of uniformly distributed heat conduction plates 11 are fixedly connected inside the heat conduction cover 10, a driving box 15 is fixedly connected to the lower end of the box body 1, a bevel gear I12 is fixedly connected to one end of the rotating shaft 3, and a speed reduction motor 13 is fixedly connected inside the driving box 15, the tail end of an output shaft of the speed reducing motor 13 is fixedly connected with a second bevel gear 14, the outer side of the box body 1 is hinged with a box door 17, the first bevel gear 12 is meshed with the second bevel gear 14, and the heat conducting plates 11 can uniformly dissipate heat to the inside of the box body 1.
Referring to fig. 1, 2 and 4, one end of the spring 6 is fixedly connected to the clamping block 7, the other end of the spring 6 is fixedly connected to the fixing box 4, the other end of the square rod 5 is fixedly connected to the limiting plate 8, the limiting plate 8 contacts with the fixing box 4, and the spring 6 can push the clamping block 7 to fix the culture dish inside the box body 1 through elastic force.
Referring to fig. 1, four support legs 16 are fixedly connected to the lower end of the driving box 15, the four support legs 16 are uniformly distributed at the lower end of the driving box 15, and the support legs 16 support the driving box 15.
The specific implementation process of the invention is as follows: when using, promote clamping block 7, make spring 6 compressed, then put into the inside round hole of fixed box 4 with the culture dish inside, then loosen clamping block 7, spring 6 resets, spring 6 promotes clamping block 7 through elasticity and fixes the culture dish in the inside of fixed box 4, then start constant temperature heater 9, constant temperature heater 9 transmits the inside of heat-conducting plate 11 through heat conduction cover 10 with the heat, the inside of heat-conducting plate 11 is dispersed to the heat of inside to box 1 that heat-conducting plate 11 is even, when constant temperature heater 9 heats the inside of box 1, start gear motor 13, gear motor 13 drives bevel gear 12 through bevel gear two 14 and rotates, bevel gear two 12 drives fixed box 4 through pivot 3 and rotates, fixed box 4 drives the pivoted in-process of culture dish, can make being heated of culture dish more even.
The flocculant is produced according to examples 1 to 3 and comparative examples 1 to 3, wherein the main components of straw fermentation liquor and gel are removed in the comparative examples 1 to 3, six groups of flocculants are obtained in the examples 1 to 3 and the comparative examples 1 to 3, then the six groups of prepared flocculants are placed under the same press, the pressure detection is carried out on the culture medium by using the same pressure, and the precipitation is carried out by placing sewage with the same concentration and volume, so that the following results are obtained:
from the above tables, it can be seen from examples 1-3 and comparative examples 1-3 that the addition of the straw fermentation broth and the flocculant of the gel significantly reduces the time required for flocculation, achieving the objective design objective of the present invention: through the formula design of the invention, the crushing strength and the flocculation rate of the flocculant are effectively enhanced, the straw fermentation liquor is effectively utilized, the environment and the like are protected, and the production cost is reduced.
Claims (9)
1. The degradable bioflocculant is characterized by comprising the following materials in parts by weight: 50-60 parts of straw fermentation liquor, 30-40 parts of glucose, 0.5-1.5 parts of magnesium sulfate, 1-2 parts of monopotassium phosphate, 20-30 parts of agar, 5-15 parts of gel and efficient flocculant producing bacteria.
2. The preparation method of the degradable biological flocculant is characterized by comprising the following steps:
firstly, preparing specified parts of straw fermentation liquor, glucose, high-efficiency flocculant producing bacteria, magnesium sulfate, monopotassium phosphate, agar, gel and high-efficiency flocculant producing bacteria.
And step two, placing the straw fermentation liquor into a high-temperature sterilization tank for high-temperature sterilization treatment, and cooling the straw fermentation liquor after sterilizing the sundry bacteria in the straw fermentation liquor.
And step three, putting the cooled straw fermentation broth, glucose, magnesium sulfate, potassium dihydrogen phosphate and agar into a mixing device, and fully mixing to obtain the culture medium.
And step four, inoculating the efficient flocculant producing bacteria into the culture medium, and then putting the culture medium into an incubator for constant-temperature culture.
And step five, after the cultured high-efficiency flocculant culture solution is prepared, putting the culture solution into a centrifuge, and separating the high-efficiency flocculant in the centrifuge, so that the preparation of the high-efficiency flocculant can be finished.
3. The method for preparing the degradable biological flocculant according to claim 2, wherein the straw fermentation liquid in the first step is prepared from one or more of wheat straw, corn straw and rice straw.
4. The method for preparing the degradable bioflocculant according to claim 2, wherein the temperature of the high temperature sterilization tube in the second step is 130 ℃ and the sterilization time is 15 minutes.
5. The method for preparing the degradable biological flocculant according to claim 2, wherein the temperature of the incubator in the fourth step is 40 ℃ and the incubation time is two days.
6. The method for preparing the degradable bioflocculant according to claim 2, wherein the temperature of the centrifuge in the fifth step is 10 ℃.
7. A preparation device of a degradable bioflocculant comprises a box body and is characterized in that a bearing is fixedly sleeved inside the lower end of the box body, a rotating shaft is fixedly sleeved inside the bearing, a plurality of fixing boxes which are uniformly distributed are fixedly connected to the outer side of the rotating shaft, four square rods which are uniformly distributed are slidably connected inside each fixing box, a spring is arranged outside each square rod, one end of each square rod is fixedly connected with a clamping block, three constant temperature heaters which are uniformly distributed are fixedly connected inside the box body, the inner sides of the three constant temperature heaters are fixedly connected with a heat conduction cover together, a plurality of heat conduction plates which are uniformly distributed are fixedly connected inside the heat conduction cover, a driving box is fixedly connected to the lower end of the box body, a first bevel gear is fixedly connected to one end of the rotating shaft, and a speed reduction motor is fixedly connected inside the driving box, the tail end of an output shaft of the speed reducing motor is fixedly connected with a bevel gear II, the outer side of the box body is hinged with a box door, and the bevel gear I is meshed with the bevel gear II.
8. The apparatus for preparing a biodegradable bioflocculant according to claim 7, wherein: one end of the spring is fixedly connected with the clamping block, the other end of the spring is fixedly connected with the fixed box, the other end of the square rod is fixedly connected with a limiting plate, and the limiting plate is in contact with the fixed box.
9. The apparatus for preparing a biodegradable bioflocculant according to claim 7, wherein: the lower extreme fixedly connected with four supporting legs of drive case, four the supporting leg is in the lower extreme evenly distributed of drive case.
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CN215250801U (en) * | 2021-04-25 | 2021-12-21 | 武汉中科标测科技有限公司 | Biochemical incubator of environmental biological detection |
CN215404265U (en) * | 2021-06-18 | 2022-01-04 | 韩元浩 | Energy-saving type multi-element cooperative room temperature control device |
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