CN109851061A

CN109851061A - A kind of microbial treatment method of biogas slurry and its bio-feritlizer of preparation

Info

Publication number: CN109851061A
Application number: CN201910091541.2A
Authority: CN
Inventors: 叶荫人
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-01-30
Filing date: 2019-01-30
Publication date: 2019-06-07

Abstract

The present invention relates to a kind of microbial treatment methods of biogas slurry, i.e., the organic matter containing Geobacillus stearothermophilus are added in biogas slurry and ferment, and generating high temperature using fermentation makes its moisture dissipated in biogas slurry that overflows.The Geobacillus stearothermophilus is Geobacillus stearothermophilus (Geobacillus stearothermophilus) R2D2-6.626069934, it is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC NO.15478.The organic matter can be organic waste, be effectively utilized agricultural wastes, sufficiently decomposing the elements such as nitrogen phosphorus therein makes its ionization, utilization easy for plant to absorpt.The biogas slurry waste water of raiser has more sufficiently been handled, can become processing after the moisture that evapotranspires by it by bulky liquid conveniently and be rich in the organic fertilizer solid of nutritional ingredient.

Description

A kind of microbial treatment method of biogas slurry and its bio-feritlizer of preparation

Technical field

The invention belongs to agricultural wastes biotechnologys to utilize field, be related to a kind of biogas slurry microbial treatment method and its The organic microbial fertilizer of preparation.

Background technique

In recent years, in order to meet people to meat, bird egg food is growing the needs of, China livestock and poultry breeding industry hair Rapidly, investigation display, the livestock and poultry cultivation quantity of each department all increases comparatively fast, and average growth rate is even up to 12% for exhibition.Country is energetically Livestock and poultry breeding industry is helped, modernization livestock and poultry breeding industry is grown rapidly, and has become most active economic increasing in China's rural economy Long point and important pillar industry.These large-scale plant that raises because have many advantages, such as the culture-cycle it is short, it is at low cost, be easily managed and It has been favored by people, but they also accordingly bring many problems.The livestock and poultry cultivation amount of scale livestock farming presents apparent Ascendant trend, so its bring environmental problem is also increasingly prominent, wherein representative is contaminated wastewater, at feces of livestock and poultry The foul smell problem generated in reason and breeding process.Wherein, poultry excrement and urine disposal has water to rush excrement, does cleaning up excrement and bubble excrement technique, Water rush excrement technique be the 1980s China from it is external introduce large scale of pig farm technology and management method when use it is main clear Excrement mode can keep the clean environment in pig house, be conducive to animal health.Labor intensity is small, and labor efficiency is high, is also beneficial to support A workers ' health is grown, is more applicable in the area of lack of labor, but pollutant concentration is high, COD is 11000~13000mg/ L, BOD are 5000~6000mg/L, and SS is 17000~20000mg/L.And after being separated by solid-liquid separation, most of soluble organic matter And microelement etc. stays in sewage, the pollutant concentration in sewage is still very high, and the solids nutrient content isolated is low, Manurial value is low.It is uncomplicated in the technology, but be affected by climate change in the north, winter aeration tank easily freezes, and sewage It handles part capital expenditure and power consumption is very high.Therefore livestock breeding wastewater due to discharge amount is big, processing difficulty is big gradually at For people's focus of attention.The wastewater discharge of large-scale plant that raises is big and concentrates, and high organic content, nitrogen contain in these waste water Amount is high, has antibiotic and pathogen residual.Organic matter and nitrogen content height easily cause water eutrophication, to destroy water body ring Border.So livestock breeding wastewater needs to be effectively treated before discharging, serious environmental pollution otherwise can be caused to environment, very To the ecological safety for threatening locality.Therefore, livestock breeding wastewater is concentrated and effectively handling is very important.

By taking the cultivation of certain pig breeding Co., Ltd of Hebei as an example, cultivation number is about 2400 bull of boar, child care pig 5000 Bull, 6000 bull of growing and fattening pigs, the following scheduled target are boar 6000 or more.Pigsty side is rinsed using high temperature and pressure Formula, routine disinfection are 1 Mondays time, and 1 part of solid manure in generated biogas slurry, washes by water and the liquid such as urine account for 9 parts, daily The quantity that must be handled is 250 tons.Existing 4 solid-liquid separating machines, 2 contaminant filter machines, 3 aerators, sewage treatment building It also needs to add certain flocculant and is handled and since generated biogas slurry moisture is big with equipment investment 10,000,000, it is per ton At least 20,000 yuan of flocculant cost.Under winter north temperature, aeration tank, which freezes, can not handle sewage.

Wastewater treatment has become the third-largest pollution entering the water after industrial water pollution, life water pollution in biogas slurry, in institute Have and belong to highest difficulty in resource processing, recycling is also infeasible.If making original pollutant after moisture benefit is dissipated Solid is become from liquid, relative volume and can processing mode will become flexibly very much.

Summary of the invention

In view of this, the purpose of the present invention is to provide the organic micro- of a kind of microbial treatment method of biogas slurry and its preparation Bio-feritlizer.

In order to achieve the above objectives, the invention provides the following technical scheme:

1. the organic matter containing Geobacillus stearothermophilus is added in biogas slurry and sends out by a kind of bioremediation of biogas slurry Ferment utilizes the moisture in the excessive scattered biogas slurry of fermentation heat production.

Further, the Geobacillus stearothermophilus is Geobacillus stearothermophilus (Geobacillus Stearothermophilus it is commonly micro- to be deposited in China Committee for Culture Collection of Microorganisms by) R2D2-6.626069934 Bio-Centers, deposit number CGMCC NO.15478.

Further, the organic matter is organic waste.

Further, the organic waste is mushroom residue, molasses, rice straw, corn stover, wheat stalk, dregs of beans, wine One or more of slag, acid-sludge substance.

Further, in the organic matter active quantities of Geobacillus stearothermophilus be 15,000,000,000/grams -250 hundred million/gram.

Further, the organic matter and the mass ratio of biogas slurry are 2.5-10:1.

Further, the organic matter and the mass ratio of biogas slurry are 7-10:1.

Further, the organic matter containing Geobacillus stearothermophilus adds organic matter gradually to amplify and expands by bacterial strain It arrives.

Further, the amplification,

Bioremediation described in any of the above item is handling the application in biogas slurry also within the protection scope of the present invention.

The organic fertilizer that a kind of bioremediation of biogas slurry obtains also is provided.

The beneficial effects of the present invention are: the natural pond that the present invention is generated using microbial fermentation technology processing feces of livestock and poultry cleaning Liquid, generated exothermic reaction when in particular with microorganism decomposition organic matter.In organic fertilizer digest process, in microorganism Under effect, organic matter degradation, while generating heat and releasing carbon dioxide, form humus.Heat and carbon dioxide are not It generates without foundation, to consume a large amount of organic matters, so this process is also the process of organic fertilizer weight, organic substance reduction. The initial C/N of organic materials (carbon/nitrogen) generally 30 or so, it is decomposed after general control 18 or so.In digest process, nitrogen Element only has insubstantial loss, and C/N is reduced, i.e., carbonaceous material loss is bigger in digest process, releases a large amount of thermal energy.It will contain There is the organic matter of Geobacillus stearothermophilus to be added in biogas slurry to ferment, inside biogas slurry from inside to outside come the scattered biogas slurry water that overflows Point, the thermal energy for overflowing and supplementing needed for scattered biogas slurry moisture is reduced, especially seems valuable for the north of low temperature.Processing side of the invention Method is simple and effective, free of extraneous thermal effects, and entire cost is low, is suitable for raiser's industrialization promotion application.The present invention Agricultural wastes can be effectively utilized, the effect of the elements such as nitrogen phosphorus therein is given full play to.The natural pond of raiser is more sufficiently handled Liquid waste water can become processing conveniently and be rich in the organic fertilizer of nutritional ingredient by it by bulky liquid after the moisture that evapotranspires Solid.

Specific embodiment

A preferred embodiment of the present invention will be described in detail below.The experiment side of actual conditions is not specified in embodiment Method, usually according to conventional conditions or according to the manufacturer's recommendations.

Place: the cultivation base of certain pig breeding Co., Ltd of Hebei.

Biogas slurry: 10% waste solid, 90% liquid (water and urine), maximum sewage quantity are 250T (ton).

Microbial strains: Geobacillus stearothermophilus (Geobacillusstearothermophilus) R2D2- 6.626069934, and China Committee for Culture Collection of Microorganisms's common micro-organisms center is deposited on March 22nd, 2018 (abbreviation CGMCC, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), deposit number are CGMCC No.15478, classification naming For Geobacillus stearothermophilus (Geobacillus stearothermophilus).The bacterial strain is to State Intellectual Property Office Apply for patent of invention, application number 2018105418820；It is kept during 60 DEG C of compost fermentation of the bacterial strain energy poultry waste or more high temperature Activity and mass propagation.

Embodiment 1

Bacterial strain amplification is opportunistic pathogen

A. it after 500g starch ground, will be added after peptone 500g is sufficiently stirred plus water 1200g carry out pyrohydrolysis, cooling To 40-50 DEG C；It is 40-45 DEG C preferred；It can also be more careful powder by starch ground, to increase the surface area of contacting strain, Powder is 10 mesh or more；

B. Geobacillus stearothermophilus (Geobacillus stearothermophilus) R2D2- is added 6.626069934, inoculum concentration be 20 grams, bacterial strain concentration be 20,000,000,000/gram, stand 72 hours after being sufficiently stirred；

C. it ventilates again 48 hours, adds water 5000g, ventilate 48 hours, add water 3000g, stand 72 hours, obtain stearothermophilus Ground bacillus opportunistic pathogen, bacillus activity maintains 20,000,000,000/gram or so, 2000 grams of total amount.Air output per minute is with compost per ton 200 liters (static pressure 320mm/Aq) relatively appropriate, and air supply mode utilizes 1/2 horsepower of air supply motor, and every air-supply stops 20 points in 5 minutes Clock, continuous blowing.Further, aeration equipment can be constituted jointly by air blower, main air hose, branch wind pipe and timing controller.

Embodiment 2

Further Geobacillus stearothermophilus opportunistic pathogen is expanded as bacterium source

A. it by after mushroom residue 20kg grinding, is added after Geobacillus stearothermophilus opportunistic pathogen 2kg is sufficiently stirred and stands 48-72 Hour；

B. conductivity is detected, if not standing after between 0.6-0.8, being then sufficiently stirred again, until conductivity is 0.6- Between 0.8, add 30kg mushroom residue, stands 48-72 hours after being sufficiently stirred；

C. conductivity is detected, if not standing after between 0.6-0.8, being then sufficiently stirred again, until conductivity is 0.6- Between 0.8, add 50kg mushroom residue, ventilates 24-48 hours, be sufficiently stirred, being until conductivity can be bacterium between 0.6-0.8 Source；Moisture content is about 40-50%, and aeration condition is the same as embodiment 1.

Mushroom residue is the carbon source material of microorganism, can also be replaced with other organic matters, generally for economic benefit, so Can using agricultural organic waste as being carbon source, the organic waste that can be used for example molasses, rice straw, corn stover, Wheat stalk, dregs of beans, schlempe, acid-sludge etc..

Carbon source organic matter selects difference, and conductivity can be allowed to decline or rise, and it is meeting after fermenting that major part, which drains species all, Decline, remaining is all to rise, and most of is all to rise.

Embodiment 3

Biogas slurry treatment method

A. biogas slurry is added in bacterium source and is fermented, 2.5-10 tons of bacterium sources, which are added, by biogas slurry per ton (can also be called absorption Medium), measure moisture；Preferably it is added based on 7-10 tons of bacterium sources by biogas slurry per ton；

B. if moisture is more than or equal to 40%, turning continues fermentation until moisture obtains finished product less than 40%.

If conceivable product is organic fertilizer in step b, turning continues fermentation until moisture is less than 40%, if Purpose is the moisture in processing biogas slurry, that can continue addition biogas slurry and ferment to moisture less than 40%, is so recycled.

In the fermentation process in this stage, adsorbing medium temperature has reached 50 DEG C or more, the state in exothermic reaction, natural pond Liquid is decomposed, and moisture is by high temperature loss in atmosphere.

The moisture content of general Composting starting is about 50~60%；When the moisture content of deposit is lower than 40% or less When, compost fermentation decomposition rate can slow down because microbial activity reduces.

And work as deposit moisture content and be higher than 60%, then it is in anaerobic fermentation because moisture content is excessively high and oxygen is insufficient, causes Fermentation rate slows down.

It is that the work for undertaking decomposition is taken over using the microorganism of different decomposition characteristic in entire Composting Process.Compost The initial stage of change, stack layers temperature are about 15-45 DEG C, main its duty of department of mild mesophilic property microorganism (fungi, bacterium and radiation bacterium) in, Mainly using labile carbohydrate and starch as matrix.

When temperature is more than 45 DEG C, it is instead thermophilic microorganism that mesophile, which has been suppressed or dead, at this time, It is responsible for decomposing the dissolved organic matter for remaining (hemicellulose, cellulose, protein, lignin ...) in compost and newly being formed, 70 DEG C or more are risen to temperature, most of Thermophilic Bacterias no longer adapt to, thus enter dead or suspend mode.After hot stage, it is left Part be the organic matter of more difficult decomposition and the humus that is newly formed, microbial activity has declined at this time, therefore calorific value is reduced, cause Compost temperature decline, mesophilic property microorganism is dominant again, decomposes again to the organic matter of remaining more difficult decomposition, at this point, rotten plant Matter is continuously increased and reaches stable state, that is, tends to the decomposed stage.

Thermophilic rouge ground fat bacillus (Geobacillus stearothermophilus) R2D2-6.626069934, is protected Ensconce China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC NO.15478.Utilize the bacterium Strain can keep activity and mass propagation during compost fermentation high temperature, promote the fast decoupled of organic matter in compost, generate big The thermal energy of amount is recycled to the biogas slurry of farm with the moisture in scattered biogas slurry of overflowing.

Embodiment 4

Biogas slurry treatment method, 26-28 days in April, 2018, Hengshui City village, Hebei province

A. 20 tons of stackings of mushroom residue, moisture detector gear are set in 7, when 26 days 1220 April (indicate at 12 points 20 minutes, with Similarly hereinafter) moisture, temperature and the pH-value (pH) in the adsorbing medium that (is denoted as T1-T10 respectively) at random measurement 10, records in detail；

B. Geobacillus stearothermophilus 72kg (activity for 20,000,000,000/gram or so), forklift turning, in top end opening are added Place addition 5 tons of biogas slurry, have 0.5-0.8 ton it is excessive, place detect again every other day；It measures to adsorb at 10 at random when 26 days 2010 April and be situated between Moisture, temperature and pH-value in matter record in detail；

C.4 moisture, temperature and the pH-value at 10 in adsorbing medium are measured when 27 days 0821 moon at random, is recorded in detail；

D.4 turning when 27 days 1437 moon, random moisture, temperature and the pH-value measured at 10 in adsorbing medium, remembers in detail Record, placement detect again every other day；

E.4 moisture, temperature and the pH-value at 10 in adsorbing medium are measured when 28 days 0842 moon at random, is recorded in detail；

F.4 turning when 28 days 0922 moon, random moisture, temperature and the pH-value measured at 10 in adsorbing medium, remembers in detail Record.

It is as shown in table 1 with identifying recording layer.When adding biogas slurry, half amount can be first added, is poured again after standing 0.5-1 hours The other half is best.Such as the power 15m of submerged motor in the present embodiment³/ h, 0.25m per minute³, being counted as weight is 250kg, is poured 5T is exactly continuous 20min, can be first continuous pouring 10 minutes, continuous pouring 10 minutes again after standing 1 hour.

1 embodiment of table, 4 data record sheet

Because of the limitation of this experimental site having time and manpower, so by Geobacillus stearothermophilus, mushroom residue and biogas slurry Directly mixing carries out fermentation process.

After having added biogas slurry, temperature can first lower and increase again with fermentation, and moisture can be drawn high, then as fermentation can gradually reduce And it maintains.The effect of moisture loss is quite good as can be seen from Table 1, can evapotranspire, be added adding large quantity of moisture Biogas slurry moisture all overflows via high temperature and dissipates among air.

Finally, it is stated that preferred embodiment above is only used to illustrate the technical scheme of the present invention and not to limit it, although logical It crosses above preferred embodiment the present invention is described in detail, however, those skilled in the art should understand that, can be Various changes are made to it in form and in details, without departing from claims of the present invention limited range.

Claims

1. a kind of microbial treatment method of biogas slurry, which is characterized in that add the organic matter containing Geobacillus stearothermophilus Enter in biogas slurry and ferment, generating high temperature using fermentation makes its moisture dissipated in biogas slurry that overflows.

2. the bioremediation of biogas slurry according to claim 1, which is characterized in that the Geobacillus stearothermophilus For Geobacillus stearothermophilus (Geobacillusstearothermophilus) R2D2-6.626069934, it is deposited in State's Microbiological Culture Collection administration committee common micro-organisms center, deposit number CGMCC NO.15478.

3. the microbial treatment method of biogas slurry according to claim 1, which is characterized in that the organic matter is organic waste Object.

4. the microbial treatment method of biogas slurry according to claim 3, which is characterized in that the organic waste is mushroom One or more of slag, molasses, rice straw, corn stover, wheat stalk, dregs of beans, schlempe, acid-sludge substance.

5. the microbial treatment method of biogas slurry according to claim 1-4, which is characterized in that in the organic matter The active quantities of Geobacillus stearothermophilus are 15,000,000,000/grams -250 hundred million/gram.

6. the microbial treatment method of biogas slurry according to claim 1-4, which is characterized in that the organic matter with The mass ratio of biogas slurry is 2.5-10:1.

7. the microbial treatment method of biogas slurry according to claim 6, which is characterized in that the matter of the organic matter and biogas slurry Amount is than being 7-10:1.

8. the microbial treatment method of biogas slurry according to claim 1-4, which is characterized in that described containing thermophilic The organic matter of fatty ground bacillus by bacterial strain add organic matter gradually amplify amplification obtain.

9. application of the described in any item microbial treatment methods of claim 1-8 in processing biogas slurry.

10. the organic fertilizer that the described in any item microbial treatment methods of claim 1-8 obtain.