CN110835609A - Photosynthetic bacteria culture medium and preparation method and application thereof - Google Patents
Photosynthetic bacteria culture medium and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a photosynthetic bacteria culture medium and a preparation method and application thereof, belonging to the technical field of bacteria culture media. The photosynthetic bacteria culture medium comprises plant hormones and a basic culture medium. Compared with the traditional method for obtaining the high-efficiency photosynthetic bacteria by changing external conditions and metabolic mechanisms, the photosynthetic bacteria culture medium has the advantages that the plant hormone is added, the metabolism of cells is accelerated, the absorption of nutrients is increased, the activity of the cells is improved, the growth speed of the photosynthetic bacteria is promoted, and the photosynthetic bacteria have high growth speed and high concentration.
Description
Technical Field
The invention relates to the technical field of bacterial culture media, in particular to a photosynthetic bacteria culture medium and a preparation method and application thereof.
Background
Photosynthetic bacteria are a general term for a large group of prokaryotes which can perform oxygen-free photosynthesis by using hydrogen sulfide, ammonia and the like as hydrogen donor and carbon source under anaerobic illumination or aerobic dark conditions. The photosynthetic bacteria have complex and various metabolic functions, the thalli have rich nutrition and physiological active substances and can be used for treating organic wastewater, and meanwhile, the photosynthetic bacteria can be used as a culture water body purifying agent and bait to be added to aquaculture, so the photosynthetic bacteria have great ecological significance and application value in aquaculture.
However, the photosynthetic bacteria cultured in the traditional culture medium have long culture time and low concentration, so that the use of the photosynthetic bacteria is limited.
Disclosure of Invention
In view of the above, it is necessary to provide a photosynthetic bacteria culture medium which can promote the growth of photosynthetic bacteria and make the growth rate of photosynthetic bacteria high and the concentration high.
A photosynthetic bacteria culture medium comprises plant hormone and a basic culture medium.
Compared with the traditional method for obtaining the high-efficiency photosynthetic bacteria by changing external conditions and metabolic mechanisms, the photosynthetic bacteria culture medium is added with the plant hormone, and can accelerate the metabolism of cells, increase the absorption of nutrients and improve the activity of the cells so as to promote the growth speed of the photosynthetic bacteria.
In one embodiment, the plant hormone is selected from the group consisting of: at least one of gibberellin, triacontanol, and indoleacetic acid.
In one embodiment, the plant hormone is selected from gibberellins.
Gibberellins are plant hormones belonging to the diterpenoid acid structure, and can promote conversion of maltose (induction of α -amylase formation), promote vegetative growth, prevent organ abscission, break dormancy and the like, induce cell division and promote cell elongation.
In one embodiment, the gibberellins are used in amounts of: 288.6n mol-2887 n mol gibberellin is added in every 1000ml photosynthetic bacteria culture medium, namely 0.1-1mg, preferably 0.25-0.75mg, more preferably 0.4-0.6 mg.
In one embodiment, the minimal medium comprises the following concentrations of raw materials: 2-3 g/L sodium acetate, 1-2 g/L ammonium chloride, 0.5-1 g/L dipotassium hydrogen phosphate, 0.1-0.2 g/L magnesium sulfate, 8-10 g/L sodium chloride, 0.5-1 g/L sodium bicarbonate and 1-2 g/L yeast extract.
It is understood that the above-mentioned minimal medium is suitable for the growth of the photosynthetic bacteria in the conventional use, and can be appropriately adjusted in the use.
In one embodiment, the pH value of the photosynthetic bacteria culture medium is 7.5-8.0.
The invention also discloses a preparation method of the photosynthetic bacteria culture medium, which comprises the following steps: and (3) dissolving the rest components except water in the photosynthetic bacteria culture medium with water, adding water to a predetermined amount, and adjusting the pH value to 7.5-8.0 to obtain the photosynthetic bacteria culture medium.
The invention also discloses application of the photosynthetic bacteria culture medium in culturing photosynthetic bacteria.
The photosynthetic bacteria culture medium is used for culturing photosynthetic bacteria, and compared with the traditional culture medium, the photosynthetic bacteria prepared by the invention has higher concentration and better quality.
In one embodiment, the photosynthetic bacteria are rhodopseudomonas sp.
In one embodiment, the culture method of the rhodopseudomonas comprises the following steps: inoculating Rhodopseudomonas seed culture solution into culture medium to make its concentration be 0.5X 109-1.5×109cfu/ml, and culturing for 2-8d under the conditions of temperature of 25-35 ℃ and illumination of 2200 and 2800lx after being uniformly mixed.
Compared with the prior art, the invention has the following beneficial effects:
compared with the traditional method for obtaining the high-efficiency photosynthetic bacteria by changing external conditions and metabolic mechanism, the photosynthetic bacteria culture medium disclosed by the invention has the advantages that the plant hormone is added, and the growth speed of the photosynthetic bacteria can be promoted by accelerating the metabolism of cells, increasing the absorption of nutrients and improving the activity of the cells. Moreover, the plant growth hormone has low price, no toxic or side effect, no pollution to the environment and low dosage, so the plant growth hormone is effective for promoting the growth of the photosynthetic bacteria.
The photosynthetic bacteria culture medium is used for culturing photosynthetic bacteria, and compared with the traditional culture medium, the photosynthetic bacteria prepared by the invention has higher concentration and better quality.
Detailed Description
In order that the invention may be more fully understood, reference will now be made to specific embodiments. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
The starting materials used in the following examples are all commercially available.
Example 1
A photosynthetic bacteria culture medium is prepared by the following method:
3g of sodium acetate, 1g of ammonium chloride, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 10g of sodium chloride, 1g of sodium bicarbonate, 2g of yeast extract and 0.5mg (1443n mol) of gibberellin, adding distilled water to 1000mL of the mixture, filling the mixture into a conical flask with a plug, and adjusting the pH value to be 7.5-8.0 after the mixture is completely dissolved.
The density is about 1 × 109The rhodopseudomonas seed culture solution of cfu/ml is inoculated into the culture medium according to the proportion of 10 percent, and after being uniformly mixed, the rhodopseudomonas seed culture solution is cultured for 5 days under the conditions of the temperature of 25-35 ℃ and the illumination intensity of 2500 lx.
Example 2
A photosynthetic bacteria culture medium is prepared by the following method:
the photosynthetic bacteria culture medium is prepared by the following method: 3g of sodium acetate, 1g of ammonium chloride, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 10g of sodium chloride, 1g of sodium bicarbonate, 2g of yeast extract and 1.0mg (2278n mol) of triacontanol, adding distilled water to 1000mL of the mixture, filling the mixture into a conical flask with a plug, and adjusting the pH value to be 7.5-8.0 after the mixture is completely dissolved.
The density is about 1 × 109The rhodopseudomonas seed culture solution of cfu/ml is inoculated into the culture medium according to the volume proportion of 10 percent, and after being uniformly mixed, the rhodopseudomonas seed culture solution is cultured for 5 days under the conditions of the temperature of 25-35 ℃ and the illumination intensity of 2500 lx.
Example 3
A photosynthetic bacteria culture medium is prepared by the following method:
the photosynthetic bacteria culture medium is prepared by the following method: 3g of sodium acetate, 1g of ammonium chloride, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 10g of sodium chloride, 1g of sodium bicarbonate, 2g of yeast extract and 0.5mg (2854n mol) of indoleacetic acid, adding distilled water to 1000mL of the solution, filling the solution into a conical flask with a plug, and adjusting the pH value to be 7.5-8.0 after the solution is completely dissolved.
The density is about 1 × 109The rhodopseudomonas seed culture solution of cfu/ml is inoculated into the culture medium according to the proportion of 10 percent, and after being uniformly mixed, the rhodopseudomonas seed culture solution is cultured for 5 days under the conditions of the temperature of 25-35 ℃ and the illumination intensity of 2500 lx.
Example 4
A photosynthetic bacteria culture medium is prepared by the following method:
3g of sodium acetate, 1g of ammonium chloride, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 10g of sodium chloride, 1g of sodium bicarbonate, 2g of yeast extract and 0.1mg (288.6n mol) of gibberellin, adding distilled water to 1000mL of the mixture, filling the mixture into a conical flask with a plug, and adjusting the pH value to be 7.5-8.0 after the mixture is completely dissolved.
The density is about 1 × 109The rhodopseudomonas seed culture solution of cfu/ml is inoculated into the culture medium according to the proportion of 10 percent, and after being uniformly mixed, the rhodopseudomonas seed culture solution is cultured for 5 days under the conditions of the temperature of 25-35 ℃ and the illumination intensity of 2500 lx.
Example 5
A photosynthetic bacteria culture medium is prepared by the following method:
3g of sodium acetate, 1g of ammonium chloride, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 10g of sodium chloride, 1g of sodium bicarbonate, 2g of yeast extract and 1mg (2887n mol) of gibberellin, adding distilled water to 1000mL of the mixture, filling the mixture into a conical flask with a plug, and adjusting the pH value to be 7.5-8.0 after the mixture is completely dissolved.
The density is about 1 × 109The rhodopseudomonas seed culture solution of cfu/ml is inoculated into the culture medium according to the proportion of 10 percent, and after being uniformly mixed, the rhodopseudomonas seed culture solution is cultured for 5 days under the conditions of the temperature of 25-35 ℃ and the illumination intensity of 2500 lx.
Example 6
A photosynthetic bacteria culture medium is prepared by the following method:
3g of sodium acetate, 1g of ammonium chloride, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 10g of sodium chloride, 1g of sodium bicarbonate, 2g of yeast extract and 0.5mg (1443n mol) of gibberellin, adding distilled water to 1000mL of the mixture, filling the mixture into a conical flask with a plug, and adjusting the pH value to be 7.5-8.0 after the mixture is completely dissolved.
Bonding density of about 1X 109The cfu/ml purple non-sulfur photosynthetic bacteria seed culture solution is inoculated into the culture medium according to the proportion of 10 percent, and after being uniformly mixed, the culture medium is placed under the conditions of the temperature of 25-35 ℃ and the illumination intensity of 2500lx for 5 d.
Comparative example 1
A photosynthetic bacteria culture medium is prepared by the following method:
the photosynthetic bacteria culture medium is prepared by the following method: 3g of sodium acetate, 1g of ammonium chloride, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 10g of sodium chloride, 1g of sodium bicarbonate and 2g of yeast extract, adding distilled water to 1000mL, putting into a conical flask with a plug, and adjusting the pH value to be 7.5-8.0 after all the components are dissolved.
The density is about 1 × 109The rhodopseudomonas seed culture solution of cfu/ml is inoculated into the culture medium according to the proportion of 10 percent, and after being uniformly mixed, the rhodopseudomonas seed culture solution is cultured for 5 days under the conditions of the temperature of 25-35 ℃ and the illumination intensity of 2500 lx.
Comparative example 2
A photosynthetic bacteria culture medium is prepared by the following method:
3g of sodium acetate, 1g of ammonium chloride, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 10g of sodium chloride, 1g of sodium bicarbonate and 2g of yeast extract, adding distilled water to 1000mL, putting into a conical flask with a plug, and adjusting the pH value to be 7.5-8.0 after all the components are dissolved.
The density is about 1 × 109The cfu/ml purple non-sulfur photosynthetic bacteria seed culture solution is inoculated into the culture medium according to the proportion of 10 percent, and after being uniformly mixed, the culture medium is placed under the conditions of the temperature of 25-35 ℃ and the illumination intensity of 2500lx for 5 d.
Examples of the experiments
After culturing for 5d in the culture medium and method for photosynthetic bacteria of the above examples and comparative examples, the average value of optical density OD660 at a wavelength of 660nm was measured for each example.
TABLE 1 average optical density OD660 at 660nm for the examples and comparative examples
As shown in the table above, the addition of phytohormones to the culture media of examples 1-6 promotes the growth of various photosynthetic bacteria (e.g., Rhodopseudomonas and purple non-sulfur photosynthetic bacteria), especially gibberellin is used, the growth of various photosynthetic bacteria can be significantly promoted at 288.6n mol-1443 nmol, but the higher the gibberellin is, the more the gibberellin is, the most significant the promotion effect is, and the higher the gibberellin concentration is, the more the gibberellin concentration is preferably, the more the bacteria. .
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A photosynthetic bacteria culture medium is characterized by comprising plant hormones and a basic culture medium.
2. A photosynthetic bacteria culture medium according to claim 1 wherein the plant hormones are selected from the group consisting of: at least one of gibberellin, triacontanol, and indoleacetic acid.
3. A photosynthetic bacteria culture medium according to claim 2, characterized in that the plant hormones are selected from the group consisting of gibberellins.
4. A photosynthetic bacteria culture medium according to claim 3 wherein the gibberellin is present in an amount of: 288.6n mol-2887 n mol gibberellin is added into every 1000ml photosynthetic bacteria culture medium.
5. A photosynthetic bacteria culture medium according to any one of claims 1 to 4, characterized in that the minimal medium comprises the following concentrations of raw materials: 2-3 g/L sodium acetate, 1-2 g/L ammonium chloride, 0.5-1 g/L dipotassium hydrogen phosphate, 0.1-0.2 g/L magnesium sulfate, 8-10 g/L sodium chloride, 0.5-1 g/L sodium bicarbonate and 1-2 g/L yeast extract.
6. A photosynthetic bacteria culture medium according to claim 5 wherein the photosynthetic bacteria culture medium has a pH of 7.5 to 8.0.
7. A method for preparing a culture medium for photosynthetic bacteria according to any one of claims 1 to 6 comprising the steps of: and (3) dissolving the rest components except water in the photosynthetic bacteria culture medium with water, adding water to a predetermined amount, and adjusting the pH value to 7.5-8.0 to obtain the photosynthetic bacteria culture medium.
8. Use of a culture medium for photosynthetic bacteria according to any one of claims 1 to 6 for culturing photosynthetic bacteria.
9. Use of a photosynthetic bacteria culture medium according to claim 8 in culturing photosynthetic bacteria wherein the photosynthetic bacteria is Rhodopseudomonas sp.
10. The photosynthetic bacteria culture medium of claim 9, wherein the Rhodopseudomonas sp is cultured by the following method: inoculating Rhodopseudomonas seed culture solution into culture medium to make its concentration be 0.5X 109-1.5×109cfu/ml, uniformly mixing, and culturing at 25-35 ℃ under the illumination of 2200-。
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| WO2010113149A1 (en) * | 2009-04-02 | 2010-10-07 | Rosetta Green Ltd. | Compositions and methods for increasing oil content in algae |
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Application publication date: 20200225 |