CN103525870A - Microbial flocculant as well as preparation method and application thereof - Google Patents
Microbial flocculant as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a microbial flocculant as well as a preparation method and application thereof. The preparation method comprises the following steps: inoculating flocculant-producing bacteria in a coarse fiber raw material to prepare a fermentation substrate with water content of 50-80 percent and pH (potential of Hydrogen) of 2-7; performing aerobic fermentation and anaerobic fermentation in sequence to obtain a fermentation product; leaching the fermentation product by using alkali liquor; filtering and drying leaching liquor to prepare the microbial flocculant. The fiber raw material is biologically converted by using the specific flocculant-producing bacteria, and meanwhile, a large amount of metabolites are produced during growth of the flocculant-producing bacteria, so that the obtained microbial flocculant has higher flocculation performance; the microbial flocculant is especially suitable for high-efficiency economic treatment on low-concentration heavy metal wastewater; the waste vegetables and crop straws are used as the raw materials which are low in cost, readily available and have wide sources, so that the recycling of the large amount of waste straws is realized; no wastewater is produced during production of the microbial flocculant, energy conservation and environmental friendliness are realized, the production process is simple, and the operation is convenient.
Description
Technical field
The present invention relates to heavy metal containing wastewater treatment technical field, be specifically related to a kind of microbial flocculant and its preparation method and application.
Background technology
Heavy metal is one of principal pollutant of the industry waste water and dregs such as plating, process hides, smelting, and the heavy metal in entered environment can not be biodegradable, huge by the long term toxicity harm to biological and environment after food chain enrichment; On the other hand, the heavy metal in natural water and soil is difficult to remove, and processing cost is high.
Conventionally from the aqueous solution, the common method of removal heavy metal has chemical precipitation method, electrolytic process, ion floatation method, electrodialysis and reverse osmosis method, ferrite process, absorption method etc., as document (Peng Renyong, Cheng Baozhen, the micro-electrolysis of Fe/C-flocculent precipitation is processed the research of Copper in Electroplating Waste Water, environmental engineering journal, 2012,6 (2): 501-504) utilize the micro-electrolysis-flocculent precipitation of Fe/C by the Cu in electroplating wastewater
2+content is down to 0.32mg/L by 641.78mg/L.But these traditional methods all exist a common shortcoming, while processing the low concentration heavy metal water lower than 100mg/L, often process cost is relative with raw materials cost too high, uneconomical economically.
Biosorption process energy high-level efficiency, highly selective, the heavy metal in the complicated dilute solution of fractionation by adsorption at a high speed, having broad application prospects processing aspect the complicated heavy metal wastewater thereby of large volume lower concentration.
The Chinese patent literature that is CN1161281C as publication number discloses a kind of preparation method of the stalk sorbing material for the treatment of effluent containing heavy metal ions, comprising: (1) carries out steam explosion treatment by stalk material, makes steam puffed stalk; (2) utilize ZnCl
2or FeCl
3steam puffed stalk is carried out to chemical modification, be then dried and pulverize, thereby make adsorbent for heavy metal.
But naturally organism must pass through sedimentation, filtration or centrifugal itself and solution could being separated after adsorbing metal, and blocking pipe very easily in operational process, microbial flocculant can address the above problem.
Microbial flocculant (MBF) extraction from microbe or its meta-bolites, purifying and obtain, it not only has the flocculating properties of traditional chemical flocculation agent, and there is the advantages such as efficient, safe, pollution-free, represented one of important directions of flocculation agent research and development.The many places of microbial flocculant research at present, in laboratory level, mainly concentrate on the flocculating property research of screening, analysis of physical and chemical property and the generation flocculation agent thereof of flcos producing bacteria kind.It is raw materials for production that the preparation of present microorganism flocculation agent utilizes sugar or agricultural byproducts more, thinking according to food fermentation and bio-pharmaceuticals is researched and developed, preparation cost is high, and biomass energy transformation efficiency and utilization ratio lower, restricted suitability for industrialized production and the large-scale application of microbial flocculant.
Agricultural crop straw refers to the cauline leaf (fringe) of ripe farm crop, farm crop after results seed, the byproducts such as residual stem, leaf that can not be edible.China's agricultural crop straw quantity is large, kind is many, distribution is wide.But in recent years, along with the increase of stalk output, rural energy Structure Improvement and the application of all kinds of alternative materials, add that stalk resource is unclear, utilize present situation not clear, scattered, the volume of distributing is large, it is high to collect transportation cost, and fully utilize the reasons such as less economical, industrialization level is low, there is provincialism, seasonality, structural surplus in stalk, a large amount of stalk resources are not utilized, and waste comparatively serious.
Summary of the invention
The invention provides a kind of preparation method of microbial flocculant, the method is not only simple to operate, and starting material are cheap, and utilizes microbial flocculant that this preparation method the obtains high adsorption capacity to heavy metal.
1, a preparation method for microbial flocculant, comprising:
(1) in robust fibre raw material, inoculate flcos producing bacteria, the fermented substrate that make water content and be 50～80%, pH is 2～7;
(2) carry out successively aerobic fermentation and anaerobically fermenting, obtain fermented product;
(3) utilize alkali lye to carry out lixiviate to fermented product, dry after vat liquor filters, make described microbial flocculant;
Described flcos producing bacteria is Tabin aspergillus (Aspergillus tubingensis) CGMCC3.06402, aspergillus oryzae (Aspergillus oryzae) CGMCC3.04259, viride (Trichoderma viride) CGMCC3.5455, trichoderma harziarum (Trichoderma harzianum) CGMCC3.04264, healthy and free from worry wood mould (Trichoderma koningii) CGMCC3.2774, healthy and free from worry wood mould (Trichoderma koningii) CICC40108, aspergillus niger (Aspergillus niger) CGMCC3.4309, aspergillus niger (Aspergillus niger) CGMCC3.06194, Rhizopus stolonifer (Rhizopus stolonife) CICC40317, volume branch Mucor (Mucor circinelloides) CGMCC3.0368, at least one in ball hair shell (Chaetomium globosum) CGMCC3.04254.
Described flcos producing bacteria is at least one in Tabin aspergillus (Aspergillus tubingensis) CGMCC3.06402, viride (Trichoderma viride) CGMCC3.5455, healthy and free from worry wood mould (Trichoderma koningii) CICC40108, aspergillus niger (Aspergillus niger) CGMCC3.06194 more preferably; When selecting two or more flcos producing bacterias, should select the flcos producing bacteria from different genera, so can more effectively degrade to robust fibre raw material.
The present invention inoculates flcos producing bacteria in robust fibre raw material, utilizes above-mentioned flcos producing bacteria to carry out bio-transformation to robust fibre raw material, and flcos producing bacteria also generates a large amount of meta-bolitess simultaneously, obtains thus microbial flocculant of the present invention.
Available robust fibre raw material form is various, and the raw material of robust fibre described in the present invention is at least one in agricultural crop straw and abandoned vegetable, is preferably the mixture of agricultural crop straw and abandoned vegetable.Abandoned vegetable and agricultural crop straw blending ratio are preferably 1:1～9, more preferably 1:1～3.
The higher vegetables of fibre content such as the optional garden beet of described abandoned vegetable, Chinese cabbage, described agricultural crop straw can be selected the agricultural crop straws such as wheat, paddy rice, corn, potato class, oil plant, cotton, sugarcane, more preferably rice straw, wheat stalk or rape stalk.Beet, Chinese cabbage, rice straw, wheat stalk and rape stalk are planted comparatively extensive in China, are easy to obtain.
Agricultural crop straw can be the fresh straw that this season is harvested and stored, and also can process through seasoning (being convenient to storage); Abandoned vegetable, because water content is high, should not be store for a long time, is generally and receives and use.
For improving processing efficiency and being convenient to follow-up use, generally will after robust fibre raw material crushing, inoculate flcos producing bacteria.The breaking method of agricultural crop straw can be that hand hay cutter breaks and pulverizes, and the breaking method of abandoned vegetable can be to pulverize, and as preferably, in butt, after fragmentation, the specific surface area of robust fibre raw material is 20-100m
2/ g.
For suitable propagation environment is provided to flcos producing bacteria, need to adjust water content and the pH value of fermented substrate, as preferably, the water content of described fermented substrate is that 50～80%, pH is 2～7, as further preferred, the water content of described fermented substrate is that 65～75%, pH is 3～5.
As preferably, with dry weight basis, the inoculum size of described flcos producing bacteria is 10
6～10
10individual spore/g fermented substrate.
For ease of storing, after being made to fermenting agent, described flcos producing bacteria inoculates.The preparation method of described fermenting agent is: described flcos producing bacteria is inoculated in the stalk bran mass of sterilizing, cultivates 5～15 days for 20～30 ℃, spore quantity reaches 10
8～10
12during individual spore/g solid substrate.
The formula of described stalk bran mass is: wheat stalk powder 700g, wheat bran 300g, water 2500mL, pH nature; Mix rear 126 ℃ of sterilizing 2h.While adopting fermenting agent inoculation, the weight ratio of fermenting agent and fermented substrate (dry weight) is 1:200～2000.Fermenting process of the present invention is divided into aerobic fermentation and two stages of anaerobically fermenting.The temperature of described aerobic fermentation is 20～35 ℃.The temperature of aerobic fermentation is controlled in the scope of growth and breeding in suitable fermenting agent, is preferably 25～28 ℃.Flcos producing bacteria raised growth breeding in aerobic fermentation process, metabolism is active, produces a large amount of meta-bolitess and enzyme, and the high molecular component in matrix is degraded in a large number as Mierocrystalline cellulose, xylogen, forms a large amount of atypic low molecular compounds.
During aerobic fermentation, generally fermented substrate is piled to stockpile, to realize large scale fermentation.For guaranteeing the air capacity of stockpile inside, in aerobic fermentation process, need ventilation, ventilation can realize by regular turning or forced-ventilation.Regularly turning refers to when piling temperature rise to 60 ℃ and just stockpile is stirred, reaches the object of mixing, temperature control and ventilation; Forced-ventilation refers to vent line or passage is set in stockpile, then utilizes gas blower machinery force feed air, ventilation and the cooling of strengthening stockpile, and when the temperature of stockpile drops to room temperature and no longer raises, aerobic fermentation finishes, and carries out anaerobically fermenting.
Anaerobically fermenting is after aerobic fermentation finishes, and the operating process by the isolated air of stockpile, compresses stockpile, reduces the ventilation property of stockpile, covered with plastic film air-isolation then, and the time of anaerobically fermenting is generally 30～180 days.Microbial strains in anaerobic fermentation process does not need artificial interpolation, anaerobion can the further degree of depth be degraded to the polymers such as Mierocrystalline cellulose in matrix, the chemical reaction that simultaneously carries out corresponding complexity for the classes of compounds in system provides the sufficient reaction times, can effectively improve output and the quality product of flocculation agent.
After having fermented, utilize alkali lye to carry out lixiviate to fermented product, extraction time is 2～12h, and extraction temperature is 20～100 ℃, and the envelope-bulk to weight ratio of alkali lye and fermented product is 10～100:1.For reducing the consumption of alkali lye, the best drying of lixiviate primary fermentation thing is processed.
As preferably, described alkali lye is sodium hydroxide, potassium hydroxide or ammoniacal liquor.The concentration of alkali lye is 1%～10%, is preferably 4%～6%.
After lixiviate completes, to vat liquor filter cleaner, collect filtrate and be also dried and obtain described microbial flocculant.Filter cleaner can adopt press filtration mode to carry out, and filtrate is dry can adopt spraying dry.For improving drying efficiency, can be first to filtrate evaporation concentration, to remove most of solvent before spraying is dry.
The present invention also provides the microbial flocculant being obtained by described preparation method.Described microbial flocculant is a kind of hybrid systems that comprises the low molecular compound in a large number with different molecular weight, these low molecular compounds comprise the microbial metabolites such as amino acid, nucleic acid, also comprise the degraded product of the polymer matrixes such as cellobiose, phenolic compound.The microbial flocculant that utilizes different robust fibre raw materials to prepare, its component may be different, but its character and function are close, are mainly because all contain the functional groups such as abundant carboxyl, hydroxyl, carbonyl, quinonyl, methoxyl group.
The present invention also provides the application of described microbial flocculant in heavy metal containing wastewater treatment, and the dosage of described microbial flocculant is preferably 0.1～0.5g/L heavy metal wastewater thereby, more preferably 0.1～0.3g/L heavy metal wastewater thereby.
In heavy metal wastewater thereby monodisperse system, this microbial flocculant is brought into play the effects such as cohesion, peptization, dispersion as polyelectrolyte, its flocculation mechanism is mainly reflected in: the chemical reactions such as the functional groups such as the carboxyl containing in molecule, hydroxyl, carbonyl, quinonyl, methoxyl group and metal ion exchange, absorption, complexing, chelating, settle the metal ion in dispersion system.
As preferably, in described heavy metal wastewater thereby, the concentration of heavy metal ion is less than 100mg/L.Compare with existing process for treating heavy-metal waste water, microbial flocculant of the present invention can be obtained efficient, remarkable, economic heavy metal removal effect for the treatment of low concentration heavy metal water.
Compared with prior art, beneficial effect of the present invention is:
(1) the present invention utilizes specific flcos producing bacteria to carry out bio-transformation to lignocellulose raw material, and flcos producing bacteria generates a large amount of meta-bolitess in process of growth simultaneously, and thus obtained microbial flocculant possesses higher flocculating property, to Cr in leather-making waste water
3+clearance reach 82.9%; The efficient economy that is particularly useful for low concentration heavy metal water is processed;
(2) the present invention be take abandoned vegetable and agricultural crop straw and is raw material, and cheap and easy to get, wide material sources, realize the recyclings of a large amount of discarded stalks, and in microbial flocculant production process, do not produce waste water, and the waste residue of generation is good cultivation matrix; There are energy-conserving and environment-protective, simple, the features such as economical and efficient.
Accompanying drawing explanation
Fig. 1 is the infrared detection spectrogram of microbial flocculant in embodiment 1;
Fig. 2 is the scanning electron microscope detection figure of the flco that produces for the simulated wastewater of copper ions of the microbial flocculant of embodiment 1;
Fig. 3 is the infrared detection spectrogram of microbial flocculant in embodiment 2;
Fig. 4 is the scanning electron microscope detection figure of the flco that produces for electroplating wastewater of the microbial flocculant of embodiment 2.
Embodiment
Embodiment 1
1 fermenting agent preparation
Mould (Trichoderma koningii) CGMCC3.2774 of healthy and free from worry wood and aspergillus niger (Aspergillus niger) CGMCC3.06194 are inoculated in the stalk bran mass of sterilizing, cultivate 7 days for 28 ℃, solid substrate miospore quantity surpasses 10
10during individual spore/g, obtain fermenting agent.
The formula of described stalk bran mass is: wheat stalk powder 700g, wheat bran 300g, water 2500mL, pH nature; Mix rear 126 ℃ of sterilizing 2h.
The preparation of 2 microbial flocculants
Get fresh beet and each 10kg of fresh water rice straw, after pulverizer pulverization process, pack in stirrer, when adding water to while stirring water content of substrate and being 60% left and right, mode with spraying adds concentrated nitric acid, when substrate pH is 3-4, stop adding concentrated nitric acid, while continuing to add water to water content of substrate to 65% till.Add above-mentioned fermenting agent 40g, after stirring, take out fermented substrate, put into the plastic box of a 100cm * 100cm * 50cm, plastic box wall all has breather hole.Plastic box was placed in to 25 ℃ of good hair care ferment of thermostatic chamber after 20 days, material in case is transferred to a little plastic tank, firmly compress, keep filling fermented substrate in bucket, not spacing of bung, covers tightly bung, enters the anaerobically fermenting stage, and 25 ℃ of constant temperature are placed 60 days.
Take out fermented substrate, 90 ℃ of vacuum-dryings.Get dry matrices 10kg and insert in large plastic tank, adding concentration is 4% ammoniacal liquor 50L, and room temperature is placed 12h, every 1h, stir once during this time, by vat liquor filtering and collecting filter liquid, for guaranteeing the result of use of microbial flocculant, in filtrate, the content of suspended solid must not surpass 0.1%.After filtrate is concentrated, spraying is dried, the microbial flocculant of the preparation of must fermenting.
The infrared spectrogram of microbial flocculant is shown in Fig. 1, can read several obvious charateristic avsorption bands from Fig. 1, wherein, and 3391cm
-1be-COOH, in alcohol and phenol-the stretching vibration absorption peak of OH; 2281cm
-1be in protein and amino acid, ammonium salt compounds-NH
4 +mULTIPLE COMPOSITE bands of a spectrum; 1584cm
-1it is the stretching vibration peak of C=O in xylogen, metal carboxylate compound; 1384cm
-1symmetrical carboxylate anion-COO
-stretching vibration or-absorption peak of the in-plane bending bands of a spectrum of C-O-H or the fragrant lopps of bonding; 1027cm
-1it is the stretching vibration peak of Si-O in silicate minerals, silicon-dioxide.
3 microbial flocculants are to Cu in simulated wastewater
2+effect of settling
In 250mL Erlenmeyer flask, add 100mL simulation Cu
2+ion waste water, adds microbial flocculant 0.01g, regulation system pH to 6, the Cu after constant volume in simulated wastewater
2+concentration is 5.0ppm.Erlenmeyer flask is vibrated on 20 ℃ of constant temperature oscillators mixing, 150r/min quick oscillation 5min first, then 40r/min vibration 30min, at the centrifugal 5min of 5000r/min, obtains respectively throw out and supernatant liquor.
Cu in supernatant liquor
2+concentration is determined as 0.44ppm through ICP, the Cu of lower concentration in simulated wastewater
2+clearance is 91.2%.
The throw out obtaining is carried out to electron-microscope scanning, and scanning electron microscope (SEM) photograph as shown in Figure 2.Electronic Speculum figure by Fig. 2 can find out, the cupric ion in this microbial flocculant and simulated wastewater forms after flco, and flco individuality is little, is particulate state, and shape is comparatively regular.
4 microbial flocculants are to Cr in simulated wastewater
3+effect of settling
In 250mL Erlenmeyer flask, add 100mL simulation Cr
3+ion waste water, adds microbial flocculant 0.01g, regulation system pH to 7, the Cr after constant volume in simulated wastewater
3+concentration is 5ppm.Erlenmeyer flask is vibrated on 20 ℃ of constant temperature oscillators mixing, 150r/min quick oscillation 5min first, then 40r/min vibration 60min, removes throw out at the centrifugal 5min of 5000r/min.Cr in supernatant liquor after centrifugal
3+concentration is determined as 0.82ppm through ICP, the Cr of lower concentration in simulated wastewater
3+clearance is 83.6%.
The application of 5 microbial flocculants in leather-making waste water
Leather-making waste water is the comprehensive wastewater of tannery, and its Chrome Tanning Wastewater has been passed through alkaline purification, and mixes with other factory effluents.In 250mL Erlenmeyer flask, add 100mL leather-making waste water, add microbial flocculant 0.03g, regulation system pH to 7, the Cr after constant volume in simulated wastewater
3+concentration is 12.5ppm.Erlenmeyer flask is vibrated on 20 ℃ of constant temperature oscillators mixing, 150r/min quick oscillation 5min first, then 40r/min vibration 60min, removes throw out at the centrifugal 5min of 5000r/min.Cr in supernatant liquor after centrifugal
3+concentration is determined as 2.14ppm through ICP, the Cr in leather-making waste water
3+clearance is 82.9%.
Embodiment 2
1 fermenting agent preparation
Viride (Trichoderma viride) CGMCC3.5455 and Tabin aspergillus (Aspergillus tubingensis) CGMCC3.06402 are inoculated in respectively in the stalk bran mass of sterilizing, cultivate 5 days for 28 ℃, solid substrate miospore quantity surpasses 10
9during individual spore/g, obtain fermenting agent.
The formula of described stalk bran mass is: wheat stalk powder 700g, wheat bran 300g, water 2500mL, pH nature; Mix rear 126 ℃ of sterilizing 2h.
The preparation of 2 microbial flocculants
Get fresh beet and each 10kg of air-dry rice straw, after pulverizer pulverization process, pack in stirrer, when adding water to while stirring water content of substrate and being 60% left and right, mode with spraying adds concentrated nitric acid, when substrate pH is 3-4, stop adding concentrated nitric acid, while continuing to add water to water content of substrate to 65% till.Add above-mentioned fermenting agent 50g, after stirring, take out fermented substrate, put into the plastic box of a 100cm * 100cm * 50cm, plastic box wall all has breather hole.Plastic box was placed in to 25 ℃ of good hair care ferment of thermostatic chamber after 40 days, material in case is transferred to a little plastic tank, firmly compress, keep filling fermented substrate in bucket, not spacing of bung, covers tightly bung, enters the anaerobically fermenting stage, and 25 ℃ of constant temperature are placed 120 days.
Take out fermented substrate, 90 ℃ of vacuum-dryings.Get dry matrices 10kg and insert in large plastic tank, adding concentration is 4% NaOH50L, and room temperature is placed 12h, every 1h, stir once during this time, by vat liquor filtering and collecting filter liquid, for guaranteeing the result of use of microbial flocculant, in filtrate, the content of suspended solid must not surpass 0.1%.After filtrate is concentrated, spraying is dried, the microbial flocculant of the preparation of must fermenting.
The infrared spectrogram of microbial flocculant is shown in Fig. 3.As seen from Figure 3, the microbial flocculant that the principal character absorption peak of this microbial flocculant and embodiment 1 make is basically identical, illustrates that the component of above-mentioned two kinds of microbial flocculants is close.Wherein, 3408cm
-1be-COOH, in alcohol and phenol-the stretching vibration absorption peak of OH; 2917cm
-1cH in carbohydrate, xylogen and fatty compounds
3-and-CH
2-stretching vibration peak; 1630cm
-1it is the stretching vibration peak of C=O in xylogen, metal carboxylate compound; 1379cm
-1symmetrical carboxylate anion-COO
-stretching vibration or-absorption peak of the in-plane bending bands of a spectrum of C-O-H or the fragrant lopps of bonding; 1119cm
-1it is the stretching vibration peak of Si-O in silicate minerals, silicon-dioxide.
The application of 3 microbial flocculants in electroplating wastewater
The waste discharge of electroplating wastewater Wei Mou Electroplate Factory sewage draining exit, Cu wherein
2+, Zn
2+, total chromium concn is respectively 0.8ppm, 2.5ppm and 1.4ppm, wherein Zn
2+surpass discharging standards and require (GB21900-2008 electroplates pollutant emission standard).Power taking waste electroplating 100mL is in 250mL Erlenmeyer flask, the microbial flocculant 0.02g that adds aforesaid method to prepare, Erlenmeyer flask is vibrated on 20 ℃ of constant temperature oscillators mixing, first 150r/min quick oscillation 5min, then 40r/min vibration 60min, at the centrifugal 5min of 5000r/min, obtain respectively throw out and supernatant liquor.
Measure Cu in the supernatant liquor after centrifugal
2+, Zn
2+, total chromium concn is respectively 0.3ppm, 0.8ppm and 0.6ppm, clearance is respectively 62.5%, 68.0% and 57.1%.Each heavy metal concentration of the electroplating wastewater of processing through microbial flocculant is all lower than discharging standards.
The throw out obtaining is carried out to electron-microscope scanning, and scanning electron microscope (SEM) photograph as shown in Figure 4.As seen from Figure 4, the flco individuality that this microbial flocculant and metal ion form is little, in the form of sheets or particulate state, and shape irregularity.This flco is different from the flco structure in Fig. 2, may be that flocculation agent reacts from multiple different metal ion and produces different flcos, different flcos, and structure is not quite identical, and the accumulation body structure therefore forming is irregular.
Claims (9)
1. a preparation method for microbial flocculant, comprising:
(1) in robust fibre raw material, inoculate flcos producing bacteria, the fermented substrate that make water content and be 50～80%, pH is 2～7;
(2) carry out successively aerobic fermentation and anaerobically fermenting, obtain fermented product;
(3) utilize alkali lye to carry out lixiviate to fermented product, dry after vat liquor filters, make described microbial flocculant;
Described flcos producing bacteria is Tabin aspergillus (Aspergillus tubingensis) CGMCC3.06402, aspergillus oryzae (Aspergillus oryzae) CGMCC3.04259, viride (Trichoderma viride) CGMCC3.5455, trichoderma harziarum (Trichoderma harzianum) CGMCC3.04264, healthy and free from worry wood mould (Trichoderma koningii) CGMCC3.2774, healthy and free from worry wood mould (Trichoderma koningii) CICC40108, aspergillus niger (Aspergillus niger) CGMCC3.4309, aspergillus niger (Aspergillus niger) CGMCC3.06194, Rhizopus stolonifer (Rhizopus stolonife) CICC40317, volume branch Mucor (Mucor circinelloides) CGMCC3.0368, at least one in ball hair shell (Chaetomium globosum) CGMCC3.04254.
2. preparation method as claimed in claim 1, is characterized in that, the water content of described fermented substrate is that 65～75%, pH is 3～5.
3. preparation method as claimed in claim 1, is characterized in that, with dry weight basis, the inoculum size of described flcos producing bacteria is 10
6～10
10individual spore/2～20g fermented substrate.
4. preparation method as claimed in claim 1, is characterized in that, the temperature of described aerobic fermentation is 20～35 ℃.
5. preparation method as claimed in claim 1, is characterized in that, the time of described anaerobically fermenting is 30～180 days.
6. preparation method as claimed in claim 1, is characterized in that, described alkali lye is sodium hydroxide, potassium hydroxide or ammoniacal liquor.
7. the microbial flocculant being obtained by the arbitrary described preparation method of claim 1～6.
8. the application of microbial flocculant as claimed in claim 7 in heavy metal containing wastewater treatment, is characterized in that, the dosage of described microbial flocculant is 0.1～0.5g/L heavy metal wastewater thereby.
9. application as claimed in claim 8, is characterized in that, in described heavy metal wastewater thereby, the concentration of heavy metal ion is less than 100mg/L.
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