CN110241051A - A kind of microorganism mixed bacterial promoting plant shoot growth - Google Patents
A kind of microorganism mixed bacterial promoting plant shoot growth Download PDFInfo
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- CN110241051A CN110241051A CN201910599699.0A CN201910599699A CN110241051A CN 110241051 A CN110241051 A CN 110241051A CN 201910599699 A CN201910599699 A CN 201910599699A CN 110241051 A CN110241051 A CN 110241051A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention discloses a kind of microorganism mixed bacterials for promoting plant shoot growth, belong to agricultural microorganism technical field.The mixed bacterial is made of Bai Shi nitrogen-fixing bacteria, Anhui rhizobium and Paenibacillus polymyxa.Mentioned microorganism mixed bacterial is prepared using following methods: Bai Shi nitrogen-fixing bacteria, Anhui rhizobium and Paenibacillus polymyxa is seeded to respectively in LB culture medium, it is O.D that 30 DEG C of shaken cultivations are dense to bacterium600Then ≈ 2.0 is mixed according to the volume ratio of 1:1:1 to obtain the final product.For mixed bacterial of the invention for promoting plant shoot growth, improving crop yield has remarkable result, has broad application prospects in agricultural production.
Description
Technical field
The present invention relates to a kind of microorganism mixed bacterials for promoting plant shoot growth, belong to agricultural microorganism technology neck
Domain.
Background technique
Promoting volume increase is the top priority of modern agriculture with high efficiency.In order to realize the purpose, growing industry technical staff is past
Toward fertilizer, especially chemical fertilizer blindly is excessively used, this not only will increase planting cost, waste of resource, will also result in arable soil
The problems such as hardened acidification declines with quality of agricultural product, the face source for also seriously affecting surface water and groundwater quality of accompanying this
Pollution.Therefore, control fertilizer amount is the real needs for promoting cost-saving synergistic, energy-saving and emission-reduction, to guarantee national food security, agriculture
Product quality and safety and Agri-ecological security have a very important significance.And research and development of functional microorganism fertilizer and agricultural prebiotic
The screening compounding of bacterium is to realize one of the important means of Reducing amount of chemical fertilizer applied.
Some functional microorganisms can secrete typical plant hormone --- gibberellin near root system of plant, to promote to plant
The growth of object stem.Gibberellin (gibberellins, GA) is the Plant Hormone being widely present, and chemical structure belongs to diterpene
Class acid is obtained by four ring skeletons derivative, and physiological effect most outstanding is the elongation for promoting stem and induction long day plant in short day
Under the conditions of bolting bloom.
The functional microorganism that plant hormone can be secreted near root system of plant is known as growth-promoting rhizosphere bacterium (plant growth
promoting rhizobacteria,PGPR).It is proposed development, the exploitation of the composition and production technology composite bacteria agent of novel PGPR
It is the direction of one hot spot of present microorganism fertilizer with application.Compound purpose is between the bacterial strain made including PGPR, with
And PGPR is combined with other profitable strains, reaches the complementation of growth-promoting effect and the amplification of efficiency.
Compounding microbial inoculum is formed to secrete gibberellin bacterial strain, for promoting plant shoot growth, improves crop yield with aobvious
Effect is write, is had broad application prospects in agricultural production.
Summary of the invention
The object of the present invention is to provide a kind of microorganism mixed bacterials for promoting plant shoot growth, it is red mould by having
Three bacterial strain compositions of plain secretion capacity, respectively Bai Shi nitrogen-fixing bacteria, Anhui rhizobium and Paenibacillus polymyxa.The mixed bacterial
Have the function of synergy between multiple-microorganism, can significantly promote plant shoot growth.
The present invention specifically uses following technical scheme:
A kind of microorganism mixed bacterial promoting plant shoot growth, it is by Bai Shi nitrogen-fixing bacteria, Anhui rhizobium and more
Viscous series bacillus composition.
Wherein, Bai Shi nitrogen-fixing bacteria are Bai Shi nitrogen-fixing bacteria Azotobacter beijerinckii CGMCC9044, and China is general
Logical Microbiological Culture Collection administrative center (China General Microbiological Culture Collection
Center, CGMCC), address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date: on January 19th, 2009, preservation are compiled
Number: CGMCC No.9044.
Wherein, Anhui rhizobium are Anhui rhizobium Rhizobium anhuiense CGMCC15545, and China is common micro-
Biological inoculum preservation administrative center (China General Microbiological Culture Collection Center,
CGMCC), address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date: on December 30th, 2015, deposit number:
CGMCC No.15545。
Wherein, Paenibacillus polymyxa is Paenibacillus polymyxa Paenibacillus polymyxa CGMCC15984,
China General Microbiological culture presevation administrative center (China General Microbiological Culture
Collection Center, CGMCC), address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date: 2016 11
The moon 30, deposit number: CGMCC No.15984.
Mentioned microorganism mixed bacterial is prepared using following methods: by Bai Shi nitrogen-fixing bacteria, Anhui rhizobium and
Paenibacillus polymyxa is seeded to respectively in LB culture medium, and it is O.D that 30 DEG C of shaken cultivations are dense to bacterium600≈ 2.0, then according to 1:
The volume ratio of 1:1 mixes to obtain the final product.
Outstanding advantage of the invention is:
1. mixed bacterial of the invention is better than single bacterial strain, to plant stem growth-promoting effect better than three kinds of single bacterial strains it
With, and it is more adaptable in practical rhizosphere soil complex environment to act synergistically between strain.
2. the mixed bacterial mix proportion scheme of determining bacterial strain quantity and ratio is better than the mixed bacterial in primal environment, because matching
Stably and controllable, the definite functions in side.
3. bacteria combination is combined better than fungi.The Bacteria Culture time is shorter, and the fungal culture time is longer, therefore, bacterium training
It supports efficiency and is better than fungi.In addition, bacterium is compared with fungi, and it is stronger to the tolerance of complicated adverse circumstances, in practical rhizosphere soil
It is more adaptable in environment.
Specific embodiment
Below with reference to specific example, the present invention will be further described.
Embodiment 1
Microorganism mixed bacterial promotes hydroponic plant shoot growth
Specific implementation step is as follows:
Bai Shi nitrogen-fixing bacteria Azotobacter beijerinckii CGMCC9044 (being denoted as bacterium A), Anhui rhizobium
Rhizobium anhuiense CGMCC15545 (being denoted as bacterium B), Paenibacillus polymyxa Paenibacillus polymyxa
CGMCC15984 (is denoted as bacterium C), is seeded in LB culture medium respectively, and it is O.D that 30 DEG C of shaken cultivations are dense to bacterium600≈ 2.0, respectively
It is mixed by the volume ratio of 1:1:1,1:2:1,1:1:2,2:1:1, while it is mixed with the volume ratio of 1:1 to choose two of them bacterium respectively
It closes, then takes the single bacterium culture solution of three kinds of bacterium.Above-mentioned each culture solution is according to 106Water planting body is added in the microorganism final concentration of CFU/ml
Culture 1 week is continued at the rape rhizosphere position of system.The present invention is arranged one group of blank control and tests simultaneously, in the rape of hydroponic culture systems
Equivalent clear water is added as blank control group in rhizosphere position.The indexs such as plant height, total dry weight are detected, as shown in table 1 below.
1 microorganism mixed bacterial of table is to hydroponic plant stem Biological control result
| Plant height (cm) | Total dry weight (g) | |
| A+B+C combines 1:1:1 | 18.138 | 0.436 |
| A+B+C combines 1:2:1 | 15.243 | 0.394 |
| A+B+C combines 1:1:2 | 15.638 | 0.387 |
| A+B+C combines 2:1:1 | 16.594 | 0.385 |
| A+B combines 1:1 | 15.677 | 0.345 |
| A+C combines 1:1 | 14.705 | 0.362 |
| B+C combines 1:1 | 13.638 | 0.359 |
| Single bacterium A | 12.653 | 0.326 |
| Single bacterium B | 12.586 | 0.338 |
| Single bacterium C | 12.627 | 0.339 |
| Blank control | 11.357 | 0.307 |
By embodiment 1 it is found that three plants of bacterium all exist and optimal in 1:1:1 ratio Use Limitation fruit, for hydroponic plant, with
Blank control is compared, either the increment of plant height or total dry weight, and the effect of microorganism mixed bacterial of the invention will
Better than the sum of the effect of three kinds of single bacterial strains.
Embodiment 2
Microorganism mixed bacterial promotes earth culture plant shoot growth
Specific implementation step is as follows:
Bai Shi nitrogen-fixing bacteria Azotobacter beijerinckii CGMCC9044 (being denoted as bacterium A), Anhui rhizobium
Rhizobium anhuiense CGMCC15545 (being denoted as bacterium B), Paenibacillus polymyxa Paenibacillus polymyxa
CGMCC15984 (is denoted as bacterium C), is seeded in LB culture medium respectively, and it is O.D that 30 DEG C of shaken cultivations are dense to bacterium600≈ 2.0, respectively
It is mixed by the volume ratio of 1:1:1,1:2:1,1:1:2,2:1:1, while it is mixed with the volume ratio of 1:1 to choose two of them bacterium respectively
It closes, then takes the single bacterium culture solution of three kinds of bacterium.Above-mentioned each culture solution is according to 106Earth culture body is added in the microorganism final concentration of CFU/ml
Culture 1 week is continued at the rape rhizosphere position of system.Under the conditions of same, it is clear that equivalent is added at the rape rhizosphere position of earth culture system
Water is as blank control group.Off-test detects the indexs such as plant height, total dry weight, as shown in table 2 below.
2 microorganism mixed bacterial of table is to earth culture plant stem Biological control result
By embodiment 2 it is found that three plants of bacterium all exist and optimal in 1:1:1 ratio Use Limitation fruit, for earth culture plant, with
Blank control is compared, either the increment of plant height or total dry weight, and the effect of microorganism mixed bacterial of the invention will
Better than the sum of the effect of three kinds of single bacterial strains.This is because mixed bacterial of the invention uses particular types and proportional arrangement and three
Kind bacterial strain all has gibberellin secretion capacity, has the function of synergy, multi-cultur es phase between strain in rhizosphere in situ environment
Interaction can may also stimulate microorganism secretion more to promote the substance of shoot growth, and after the mixing of a variety of strains, microorganism
It can also interact between a variety of metabolites, promote the good metabolism of entire flora system, increase the growth-promoting functions to plant.This
Adaptability of the mixed microorganism flora of invention in practical soil environment is also stronger, has practical application dissemination.
Embodiment 3
Microbial strains secrete the measurement of gibberellin ability
Specific implementation step is as follows:
Bai Shi nitrogen-fixing bacteria Azotobacter beijerinckii CGMCC9044 (being denoted as bacterium A), Anhui rhizobium
Rhizobium anhuiense CGMCC15545 (being denoted as bacterium B), Paenibacillus polymyxa Paenibacillus polymyxa
CGMCC15984 (is denoted as bacterium C), is seeded in LB culture medium respectively, and it is O.D that 30 DEG C of shaken cultivations are dense to bacterium600≈2.0.Respectively
Three plants of bacteria culture fluid 100mL are taken, GA content therein, such as the following table 3 are measured using high performance liquid chromatography-tandem mass method.
3 strain secretes gibberellin experimental result of table
| Gibberellin secretion capacity (g/L) | |
| Bacterium A | 10.6 |
| Bacterium B | 9.8 |
| Bacterium C | 11.2 |
By embodiment 3 it is found that three plants of bacterium all have the ability of secretion gibberellin, axis can be promoted in the actual environment
Portion's growth, improves crop yield.And microorganism mixed bacterial is to plant it can be seen from the specific experiment data in Examples 1 and 2
The growth promoting function of object will be substantially better than the sum of the effect of three kinds of single bacterial strains, this may be due to the synergistic effect thorn between strain
Swash the more gibberellin of microorganism secretion, it is also more obvious to the growth promoting function of stem.
Claims (4)
1. a kind of microorganism mixed bacterial for promoting plant shoot growth, which is characterized in that it is by Bai Shi nitrogen-fixing bacteria, Anhui root
Tumor bacterium and Paenibacillus polymyxa composition；The microorganism mixed bacterial is prepared using following methods: Bai Shi is consolidated
Nitrogen bacterium, Anhui rhizobium and Paenibacillus polymyxa are seeded to respectively in LB culture medium, and it is O.D that 30 DEG C of shaken cultivations are dense to bacterium600
Then ≈ 2.0 is mixed according to the volume ratio of 1:1:1 to obtain the final product.
2. the microorganism mixed bacterial according to claim 1 for promoting plant shoot growth, which is characterized in that the Bai Shi
Nitrogen-fixing bacteria are Bai Shi nitrogen-fixing bacteria CGMCC 9044.
3. the microorganism mixed bacterial according to claim 1 for promoting plant shoot growth, which is characterized in that the Anhui
Rhizobium are Anhui rhizobium CGMCC 15545.
4. the microorganism mixed bacterial according to claim 1 for promoting plant shoot growth, which is characterized in that described mostly viscous
Series bacillus is Paenibacillus polymyxa CGMCC 15984.
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Cited By (4)
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| CN110521323A (en) * | 2019-09-29 | 2019-12-03 | 南京林业大学 | A method of locust tree of sowing grass seeds by duster on the exposed palisades of shale is efficiently replied immediately green fastly and improves soil pH value |
| CN113136354A (en) * | 2021-06-07 | 2021-07-20 | 阳煤丰喜肥业（集团）有限责任公司闻喜复肥分公司 | Microbial agent for promoting wheat growth and preparation method thereof |
| CN113174351A (en) * | 2021-06-11 | 2021-07-27 | 成都云图控股股份有限公司 | Disease-resistant growth-promoting mixed microbial flora |
| CN113748908A (en) * | 2021-08-17 | 2021-12-07 | 海南农佳佳科技发展有限公司 | Green planting method for pecans |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521323A (en) * | 2019-09-29 | 2019-12-03 | 南京林业大学 | A method of locust tree of sowing grass seeds by duster on the exposed palisades of shale is efficiently replied immediately green fastly and improves soil pH value |
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| CN113136354A (en) * | 2021-06-07 | 2021-07-20 | 阳煤丰喜肥业（集团）有限责任公司闻喜复肥分公司 | Microbial agent for promoting wheat growth and preparation method thereof |
| CN113136354B (en) * | 2021-06-07 | 2023-04-28 | 阳煤丰喜肥业（集团）有限责任公司闻喜复肥分公司 | Microbial agent for promoting wheat growth and preparation method thereof |
| CN113174351A (en) * | 2021-06-11 | 2021-07-27 | 成都云图控股股份有限公司 | Disease-resistant growth-promoting mixed microbial flora |
| CN113174351B (en) * | 2021-06-11 | 2023-04-25 | 成都云图控股股份有限公司 | Disease-resistant growth-promoting mixed microbial flora |
| CN113748908A (en) * | 2021-08-17 | 2021-12-07 | 海南农佳佳科技发展有限公司 | Green planting method for pecans |
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