CN110250193B

CN110250193B - Pesticide composition containing kasugamycin and polyoxin

Info

Publication number: CN110250193B
Application number: CN201910715939.9A
Authority: CN
Inventors: 陈劲礼; 李新杰; 刘晓鹏; 张勤; 梁明龙; 陈悦; 刘晓静; 陈绍嘉
Original assignee: Jiangmen Plant Protection Co ltd
Current assignee: Jiangmen Plant Protection Co ltd
Priority date: 2019-08-02
Filing date: 2019-08-02
Publication date: 2021-08-24
Anticipated expiration: 2039-08-02
Also published as: CN110250193A

Abstract

The invention belongs to the field of pesticides, and particularly relates to a pesticide composition containing kasugamycin and polyoxin, wherein the weight ratio of the kasugamycin to the polyoxin is 1:25-25:1, the composition can be used for preventing and treating crop diseases, particularly tomato leaf mold and tomato gray mold, and has an obvious synergistic effect.

Description

Pesticide composition containing kasugamycin and polyoxin

Technical Field

The invention belongs to the field of pesticides, and particularly relates to a pesticide composition containing kasugamycin and polyoxin.

Background

Kasugamycin belongs to an antibiotic bactericide, has protection and treatment effects, stronger systemic activity, strong permeability and strong mobility, can move in a plant body, has obvious treatment effect, takes effect quickly after spraying, is resistant to rain wash, has long lasting period, and can be used as an ideal medicament for preventing and treating various bacteria and fungal diseases such as rice blast, tomato leaf mold, powdery mildew, cucumber bacterial angular leaf spot, cabbage black rot, fruit and vegetable anthracnose and the like on crops such as vegetables, melons, fruits, rice and the like.

Polyoxin is a nucleoside antibiotic separated from cacao streptomycin axologym, is a metabolite of streptomyces aureochromogenes in China, is a mixture consisting of 14 different homologs from A to N, has a main active component polyoxin B, belongs to polyoxin nucleoside agricultural antibiotic bactericides, can effectively inhibit the synthesis of a fungal cell wall skeleton component-chitin, and can prevent bacterial cell walls from being biosynthesized to cause the death of germs. Polyoxin is a safe pesticide with high efficiency, low toxicity and no environmental pollution, so that polyoxin is widely applied to the control of important diseases of grain crops, special crops, fruits, vegetables and the like.

The use of chemical pesticides is reduced, the adoption of green and environment-friendly biological pesticides for preventing and treating diseases has become a necessary trend of pesticide research and development, and in addition, the biological activity of the biological pesticides is relatively general compared with that of the chemical pesticides under the normal condition, so that the compounding of the biological pesticides for enhancing the prevention and treatment effect, expanding the prevention and treatment spectrum and slowing down the generation of drug resistance is a hotspot for the prevention and treatment research of pollution-free pesticides at the present stage. In the prior art, no report about compounding kasugamycin and polyoxin is available.

Disclosure of Invention

In order to solve the problems in the prior art, the invention provides an environment-friendly pesticide composition. The invention provides the following technical scheme:

a pesticide composition containing kasugamycin and polyoxin has a weight ratio of the kasugamycin to the polyoxin of 1:25-25: 1.

The weight ratio of the kasugamycin to the polyoxin of the pesticide composition is 1:15-10: 1. Preferably 5: 1.

The pesticide composition is characterized in that the percentage content of the active ingredients is 1-65%. Preferably 5 to 35%.

The pesticide composition is characterized in that the dosage form of the composition can be wettable powder, water dispersible granules or water aqua. Suitable auxiliaries and excipients in the compositions according to the invention are all customary formulation adjuvants or components, such as carriers, surfactants, solvents, stabilizers, synergists, defoamers, thickeners, water and inert fillers. Such adjuvants are known in the art and are commercially available. Their use in the formulation of compositions of embodiments of the present invention will be apparent to those skilled in the art.

The pesticide composition is used for preventing and treating crop diseases, and the crop diseases are preferably tomato leaf mold and tomato gray mold.

Has the advantages that:

1. in the active components of the composition, the active components are all green environment-friendly biological pesticides, and are environment-friendly and safe.

2. The composition has obvious synergistic effect on prevention and treatment of crop diseases, reduces the dosage of pesticide preparations and reduces the production cost. Overcomes or delays the drug resistance of the pests and prolongs the use of the medicament.

Detailed Description

The present invention is further illustrated by the following examples, but is not limited thereto. The experimental procedures, in which specific conditions are not noted in the following examples, are generally carried out under conventional conditions or conditions recommended by the manufacturers.

Example 1 kasugamycin and polyoxin combination for indoor virulence determination of Botrytis cinerea

Test subjects: botrytis cinerea (Botrytis cinerea), collected in the field, separated and purified on an indoor PDA culture medium, and then stored.

The test method comprises the following steps: hypha growth rate method. Under aseptic condition, punching the edge of the pre-cultured 3d colony of Botrytis cinerea with a punch having a diameter of 5mm, inoculating to the PDA plate containing test agent and no medicine, repeating each treatment for 3 times, culturing in dark at 25 deg.C, measuring the diameter (mm) of each treated colony after 3d by cross method, calculating the inhibition rate, and determining the effective inhibition medium concentration (EC) for the test agent to inhibit the growth of hypha by DPS₅₀Value).

The CTC calculation method comprises the following steps: the co-toxicity coefficient (CTC) was calculated according to the Sun cloud Peter method. When CTC is less than 80, the composition is antagonistic, when CTC is less than 80, the composition is additive, and when CTC is less than 120, the composition is synergistic. Measured virulence index (ATI) ═ standard agent EC₅₀Reagent for test EC₅₀) X 100; theoretical virulence index (TTI) ═ a agent virulence index × percentage of a in the mixture + B agent virulence index × percentage of B in the mixture; co-toxicity coefficient (CTC) ═ mix measured virulence index (ATI)/mix theoretical virulence index (TTI)]×100。

TABLE 1 kasugamycin and polyoxin combination for indoor virulence determination of Botrytis cinerea

Medicament	Proportioning	EC₅₀Value mg/L	CTC value
				Kasugamycin A	-	3.65	-
Polyoxin B	-	6.30	-
				A:B	1:25	5.71	107.3
A:B	1:15	4.50	133.9
				A:B	1:10	4.28	138.1
A:B	1:5	4.33	129.8
				A:B	1:1	3.49	132.4
A:B	5:1	2.57	152.7
				A:B	10:1	2.88	131.8
A:B	15:1	3.13	119.8
				A:B	25:1	3.17	117.0

As can be seen from Table 1, the co-toxicity coefficients of the indoor activity tests of the kasugamycin and the polyoxin for the tomato gray mold are all larger than 130 in the range of 1:15-10:1, which shows that the compound has obvious synergistic effect on the tomato gray mold.

Example 2 formulation example (the following formulation preparation methods were carried out in a manner conventional in the art)

112% kasugamycin-polyoxin wettable powder preparation

210% kasugamycin-polyoxin water dispersible granule

Components	Percentage content%
		Kasugamycin	5
Polyoxins	5
		TERSPERSE 2700	7
Morwet D-425	2
		Sodium carboxymethylcellulose	3
Ammonium sulfate	3
		Bentonite clay	Complement 100

Preparation of 35% kasugamycin-polyoxin aqueous solution

Example 3 field control of kasugamycin and polyoxin combination against tomato leaf mold

Test subjects: leaf mold

And (3) test crops: tomato

The test method comprises the following steps: the method is carried out in a sunlight greenhouse with serious tomato leaf mold, the treatment is repeated for 4 times, and the block design is completely random, and a protection row is arranged. The plants are treated by drug application after disease onset, and are used for measuring the treatment effect and investigating the disease base before drug application. The test is carried out by applying the medicine for 1 time at intervals of 7d and applying the medicine for 2 times in total, investigating the disease condition of plants before applying the medicine and 10d after applying the medicine for the last time, and calculating the disease condition index and the prevention effect.

Disease index ═ Σ (disease grade number × disease grade leaf number)/(investigation total leaf number × 9) × 100;

the treatment effect is [1- (disease index before control group medicament x disease index after treatment group medicament)/(disease index after control group medicament x disease index before treatment group medicament) ] × 100%.

The investigation method comprises the following steps: sampling is carried out at 3 points in each time of investigation, 1 compound leaf is taken from each plant at 5 points, and the lesion area of each small leaf on each compound leaf is classified according to the percentage of the lesion area of each small leaf on the whole small leaf. The classification standard is as follows:

level 0: no disease spots;

level 1: the lesion area is less than 5 percent;

and 3, level: the area of the lesion is more than or equal to 5 percent and less than 10 percent;

and 5, stage: the area of the lesion is more than or equal to 10 percent and less than 20 percent;

and 7, stage: the area of the lesion is more than or equal to 20 percent and less than 50 percent;

and 9, stage: the area of the lesion is more than or equal to 50 percent.

TABLE 2 field control of kasugamycin and polyoxin combinations against tomato leaf mold

As can be seen from the table 2, the kasugamycin and the polyoxin can effectively prevent and treat the tomato leaf mold after being compounded, the prevention and treatment effects are superior to those of a control medicament, the prevention and treatment effects are greatly improved under the condition of reducing the using amount, and no adverse effect is caused on target crops in the test medicament application range.

Furthermore, it should be understood that various changes and modifications can be made by those skilled in the art after reading the above disclosure, and equivalents also fall within the scope of the invention as defined by the appended claims.

Claims

1. The application of the pesticide composition containing kasugamycin and polyoxin in preventing and treating tomato gray mold is characterized in that the pesticide composition is wettable powder and comprises the following components:

kasugamycin 10%

Polyoxin 2%

Sodium dodecyl benzene sulfonate 10%

BX 5% of nekal

Light calcium carbonate 5%

Kaolin makes up to 100%.