CN115968898A - Pseudo-ginseng seed strip seed dressing agent and method for promoting pseudo-ginseng growth - Google Patents
Pseudo-ginseng seed strip seed dressing agent and method for promoting pseudo-ginseng growth Download PDFInfo
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Abstract
The invention relates to a seed dressing agent for pseudo-ginseng seed sticks and a method for treating the seed sticks to promote the growth of pseudo-ginseng, belonging to the field of medicinal plant cultivation. The pseudo-ginseng seed strip seed dressing agent comprises: tetramycin powder, 10% polyoxin wettable powder and 2% kasugamycin wettable powder. The invention also provides a method for promoting the growth of pseudo-ginseng by using the pseudo-ginseng seed strip seed dressing agent and application of the pseudo-ginseng seed strip seed dressing agent in pseudo-ginseng planting.
Description
Technical Field
The invention relates to a seed dressing agent for pseudo-ginseng seed sticks and a method for treating the seed sticks to promote the growth of pseudo-ginseng, belonging to the field of medicinal plant cultivation.
Background
Pseudo-ginseng (Panax notoginseng (Burk.) F.H.Chen) is one of important genuine Chinese medicinal materials in Yunnan province, and the root, stem, leaf and flower of the pseudo-ginseng can be used as medicines, has various effects of promoting blood circulation to remove blood stasis, relieving swelling and relieving pain, and the like, and has large market demand.
At present, the pseudo-ginseng production mode mainly comprises a traditional planting mode, a facility production mode and an original ecological planting mode under forests. The health management relationship of strong seedlings and seedlings is that the pseudo-ginseng is stored, and the health management relationship plays an important role in the high yield and income increase of pseudo-ginseng in the later growth period; the occurrence of root rot in the underground part is the key for influencing the survival rate of panax notoginseng, so that the effective treatment of panax notoginseng seed sticks is the prerequisite and guarantee for ensuring the survival of panax notoginseng seed sticks and promoting the seedling survival rate and growth of panax notoginseng.
The pseudo-ginseng seed strip treatment has the advantages of preventing and controlling diseases of crops in seedling stage, activating endogenous substances in seeds to enhance stress resistance, promoting germination and the like, the chemical treatment method is the most common mode, if the pseudo-ginseng seed strip is not used properly, problems of environmental pollution, pesticide residue and the like are easily caused to influence food safety, and the green pollution-free seed treatment has an important application prospect in production. The biological pesticide is a biological preparation prepared by extracting active ingredients from organisms, has the advantages of small toxic and side effects, less residue, easy degradation, no harm to natural enemies and the like, and has important application prospect for replacing chemical pesticides.
According to the traditional planting research report of panax notoginseng, the bio-control fungicide is adopted to limit the root rot of panax notoginseng seeds by dressing; at present, pseudo-ginseng seed strips are treated mainly by depending on chemical agents in production, for example, the mixed agent of Fumeishui, carbendazim, bisultenin and No. 2 Luheng, no. 1, fumeishui and bisultenin are mixed and mixed according to a certain proportion to treat the pseudo-ginseng seed strips, the emergence rates are 83.23 percent and 86.96 percent respectively, and the incidence rates of root rot are 10.52 percent and 10.70 percent; patent CN106066877A discloses a method for compounding 1% -20% of bactericide, 3% -30% of insecticide and 0.1% -1% of thickening agent, wherein the seedling survival rates of pseudo-ginseng seeds treated by a dry mixing method, a seed soaking method and a spraying method are 72.4%, 81.6% and 96% respectively.
The tetramycin is a metabolite of streptomyces hygroscopicus paraspinalis subspecies, and the main active ingredients comprise four components of A1, A2, B and C, wherein the two components of A1 and A2 belong to polyene macrolide tetraene antibiotics; the component B is the benoricin, which belongs to cyclopeptide antibiotics, and the component C is the anisomycin, which belongs to nitrogen heterocyclic and aromatic derivative antibiotics. The research shows that tetramycin has high inhibitory activity to various plant pathogenic bacteria, low toxicity, strong systemic property, high bactericidal activity and wide bactericidal spectrum, and has extremely strong killing effect on ascomycetes, basidiomycetes, deuteromycetes, gram negative bacteria and gram positive bacteria. Patent CN109392929A discloses a bactericidal composition containing tetramycin and kasugamycin for preventing and treating rot diseases of fruit trees, such as panax notoginseng black spot, etc.; patent CN106912490A discloses a tetramycin-containing high-efficiency bactericidal composition which is prepared from tetramycin, polyoxin, kasugamycin, ningnanmycin and tebuconazole and is used for preventing and treating rot diseases, such as panax notoginseng black spot.
Disclosure of Invention
The invention screens a biological source pseudo-ginseng seed strip seed dressing agent compound formula through field practice, not only solves the environmental problem caused by treating pseudo-ginseng seed strips with chemical agents, but also promotes the problems of seedling storage and high-quality growth of pseudo-ginseng seed strips.
The first aspect of the present invention provides a pseudo-ginseng seed stick seed dressing agent, which comprises: tetramycin powder, 10% polyoxin wettable powder and 2% kasugamycin wettable powder.
In one embodiment, the mass ratio of the tetramycin powder to the 10% wettable polyoxin powder to the 2% wettable kasugamycin powder is 40-80%:15-35%:5 to 25 percent. Preferably, the mass ratio of the tetramycin powder to the 10% polyoxin wettable powder to the 2% kasugamycin wettable powder is 50-70%:20-30%:10 to 20 percent. In a further embodiment, the mass ratio of the tetramycin powder to the 10% wettable polyoxin powder to the 2% wettable kasugamycin powder is 60%:25%:15 percent.
In another embodiment, the tetramycin powder is prepared by the following steps: and (3) uniformly mixing 0.3% (g/100 ml) tetramycin water solution and 2000-mesh quartz sand according to the volume mass ratio of 1.
In the present invention, the wettable powder of polyoxin in 10% by weight and the wettable powder of kasugamycin in 2% by weight are either self-prepared or commercially available.
The second aspect of the invention provides a method for promoting the growth of panax notoginseng by using the panax notoginseng seed strip seed dressing agent, which comprises the following steps:
(1) Compounding 0.3% tetramycin powder, 10% polyoxin wettable powder and 2% kasugamycin wettable powder, and mixing uniformly according to a proportion to obtain pseudo-ginseng seed strip seed-dressing powder;
(2) Adding a small amount of healthy pseudo-ginseng seed strips and a seed dressing agent for multiple times according to the dosage of 300 g/mu, and stirring in a rolling way;
(3) And (5) immediately transplanting the seed strips of the panax notoginseng after seed dressing treatment and covering soil.
The third aspect of the invention is to provide the application of the pseudo-ginseng seed strip seed dressing agent in pseudo-ginseng planting.
Has the advantages that: the tetramycin is converted from the aqueous solution into the solid preparation for use for the first time, and the compound formula of the pseudo-ginseng seed strip seed dressing agent is obtained through a large amount of field screening.
Drawings
FIG. 1: the influence of seed dressing agent treatment on the seedling emergence of panax notoginseng is analyzed for the significance of the difference by utilizing single-factor variance analysis and multiple comparisons. Where a, b represent the significance of the difference in the results, a and b are labeled on two different legends to indicate significant difference (p < 0.05), and the presence of the same letter indicates insignificant difference (p > 0.05).
FIG. 2: the influence of the treatment of the seed dressing agent on the survival of the panax notoginseng seedlings is analyzed by the single-factor variance analysis and multiple comparisons for the significance of the difference. Where a, b represent the differential significance of the results, a and b are marked on two different legends to indicate significant differences (p < 0.05), and the presence of the same letter indicates insignificant differences (p > 0.05).
FIG. 3: the influence of the treatment of the seed dressing agent on the biomass of the panax notoginseng is analyzed by single-factor variance analysis and multiple comparisons for the significance of the difference. Where a, b, c and d represent the significance of the difference in the results, different letters represent significant difference (p < 0.05), and the presence of the same letter represents insignificant difference (p > 0.05).
Detailed Description
The invention may be further understood by reference to the following examples, which illustrate some methods of making or using. However, it is to be understood that these examples do not limit the present invention. Variations of the invention, now known or further developed, are considered to fall within the scope of the invention as described herein and claimed below.
In the following examples, a wettable powder of 10% by weight of polyoxin and a wettable powder of 2% by weight of kasugamycin were purchased from Japan scientific research pharmaceutical Co., ltd and Qingdao Biotech GmbH, shandong province
Example 1 preparation of a seed dressing agent for pseudo-ginseng seed sticks
Preparing tetramycin powder: mixing 0.3% (g/100 ml) tetramycin aqueous solution and 2000-mesh quartz sand uniformly according to the volume mass ratio of 1.
Compounding 0.3% tetramycin powder, 10% polyoxin wettable powder and 2% kasugamycin wettable powder according to the mass ratio of 50%:30%: mixing 20% of the above materials, and making into seed dressing powder.
Example 2 preparation of pseudo-ginseng seed stick dressing agent
Preparing tetramycin powder: mixing 0.3% (g/100 ml) tetramycin water solution and 2000-mesh quartz sand uniformly according to the mass ratio of 1.
Compounding 0.3% tetramycin powder, 10% polyoxin wettable powder and 2% kasugamycin wettable powder according to the mass ratio of 70%:20%: mixing 10% to obtain Notoginseng radix seed strip seed dressing powder.
Example 3 preparation of pseudo-ginseng seed stick seed dressing agent
Preparing tetramycin powder: mixing 0.3% (g/100 ml) tetramycin aqueous solution and 2000-mesh quartz sand uniformly according to the volume mass ratio of 1.
Compounding 0.3% tetramycin powder, 10% polyoxin wettable powder and 2% kasugamycin wettable powder according to the mass ratio of 60%:25%: mixing 15% to obtain Notoginseng radix seed strip seed dressing powder.
Example 4 Effect of seed dressing agent for seed strips of Panax notoginseng on growth of Panax notoginseng
The test method comprises the following steps:
treating seed strips of four-powder multi-spring pseudo-ginseng: adding a small amount of healthy pseudo-ginseng seed strips and the pseudo-ginseng seed strip seed dressing agent (called four-powder and multi-spring for short) prepared in example 3 according to the dosage of 300 g/mu for multiple times, and stirring in a rolling way;
treating the seed strips of the multi-spring pseudo-ginseng: according to the 10% polyoxin wettable powder: the mass ratio of the 2% kasugamycin wettable powder is 5:3, uniformly mixing (abbreviated as Duochun), adding a small amount of the healthy pseudo-ginseng seed strips and the healthy Duochun for multiple times according to the dosage of 300 g/mu, and stirring in a rolling way;
treating pseudo-ginseng seed strips of a negative control group: adding a small amount of healthy pseudo-ginseng seed strips and clear water for multiple times according to the dosage of 300 g/mu, and stirring in a rolling way;
four replicates per treatment set; each repeat cell area was 1.2m × 5m.
Planting in a base within 12 months, wherein the planting specification of the seed strips is 10cm × 15cm. And respectively carrying out investigation on seedling emergence, seedling storage rate and biomass of the pseudo-ginseng in 3-4 months of the next year and 11 months of early harvest (the biennial pseudo-ginseng is not harvested).
1) And (3) carrying out investigation on the emergence and seedling storage conditions of the panax notoginseng by adopting a cell full-coverage investigation mode, wherein the emergence rate = the number of emerging plants/sowing number 100%.
2) The seedling stock rate = 100% of the number of seedling stock strains/number of seedling emergence strains.
3) And (5) surveying the biomass of the panax notoginseng by adopting a five-point sampling mode.
And (3) test results:
1) Emergence rate: the results are shown in fig. 1, and show that the emergence rate of panax notoginseng after the treatment of the panax notoginseng seed strips can be obviously improved, wherein the emergence rate of panax notoginseng after the four-powder multi-spring treatment (84.35%) is obviously higher than that of a negative control (58.56%); higher than docanol (75.49%) and the difference is not significant.
2) Seedling survival rate: the results are shown in fig. 2, which shows 85.83% of springtime, significantly higher than 37.12% of the negative control, and higher than springtime (70.51%).
3) Biomass: as can be seen from FIG. 3, the dry and fresh weight of the overground part of the pseudo-ginseng can be significantly increased after the pseudo-ginseng seedlings are transplanted and are subjected to appropriate seed dressing treatment, wherein the weight average of the dry and fresh overground part of the pseudo-ginseng subjected to the four-powder multi-spring treatment is significantly higher than that of the overground part subjected to the negative control treatment. The fresh weight sequence of the overground part is as follows: four powdered dophun (3.63 g) > dophun (3.29 g) > negative control (2.95 g); the sequence of the dry weight of the overground part is as follows: four meals more spring (0.76 g) > more spring (0.71 g) > negative control (0.66 g).
In addition, the dry fresh weight of the underground part of the panax notoginseng in all treatments is the highest in the four-powder multi-spring treatment, and is significantly higher than that in the multi-spring and negative control treatment. The fresh weight sequence of the underground part is as follows: four-powder-more-spring (3.90 g) > more-spring (3.13 g) > negative control (3.01 g) >; the dry weights of the underground parts are ordered as: four powdered dophun (1.03 g) > dophun (0.83 g) > negative control (0.81 g).
In conclusion, the influence of negative treatment, multi-spring treatment and four-powder multi-spring treatment on the emergence rate, the seedling survival rate and the dry fresh weight of the pseudo-ginseng is analyzed, and the result shows that the compounding of 0.3% tetramycin powder, 10% polyoxin wettable powder and 2% kasugamycin wettable powder can improve the emergence rate and the seedling survival rate of the pseudo-ginseng, is beneficial to the accumulation of biomass, particularly biomass of the underground part, is a practical and effective pseudo-ginseng seed strip treatment method, and generates an obvious synergistic effect among the three.
This summary merely illustrates some embodiments which are claimed, wherein one or more of the features recited in the claims can be combined with any one or more of the claims, and such combined features are also within the scope of the present disclosure as if they were specifically recited therein.
Claims (7)
1. A pseudo-ginseng seed stick seed dressing agent, comprising: tetramycin powder, 10% polyoxin wettable powder and 2% kasugamycin wettable powder.
2. The pseudo-ginseng seed stick dressing agent according to claim 1, wherein the mass ratio of the tetramycin powder to the 10% wettable polyoxin powder to the 2% wettable kasugamycin powder is 40-80%:15-35%:5 to 25 percent.
3. The pseudo-ginseng seed stick seed dressing agent according to claim 2, wherein the mass ratio of the 0.3% tetramycin powder, the 10% wettable polyoxin powder and the 2% wettable kasugamycin powder is 50-70%:20-30%:10 to 20 percent.
4. The pseudo-ginseng seed stick dressing agent according to claim 3, wherein the mass ratio of the 0.3% tetramycin powder to the 10% wettable polyoxin powder to the 2% wettable kasugamycin powder is 60%:25%:15 percent.
5. The pseudo-ginseng seed stick dressing agent according to any one of claims 1 to 4, wherein the 0.3% tetramycin powder is prepared by the following steps: mixing 0.3% (g/100 ml) tetramycin aqueous solution and 2000-mesh quartz sand uniformly according to the proportion of 1.
6. A method for promoting the growth of pseudo-ginseng by using the pseudo-ginseng seed stick dressing agent as defined in any one of claims 1 to 4, comprising the steps of:
(1) Compounding 0.3% tetramycin powder, 10% polyoxin wettable powder and 2% kasugamycin wettable powder, and mixing uniformly according to a proportion to obtain pseudo-ginseng seed strip seed dressing powder;
(2) Adding a small amount of healthy pseudo-ginseng seed strips and a seed dressing agent for multiple times according to the dosage of 300 g/mu, and stirring in a rolling way;
(3) And (5) immediately transplanting the seed-dressing treated pseudo-ginseng seed strips and covering soil.
7. Use of the pseudo-ginseng seed stick dressing agent according to any one of claims 1 to 4 in pseudo-ginseng planting.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155934A (en) * | 2013-03-12 | 2013-06-19 | 文山学院 | Pseudo-ginseng (seedling) seed-dressing agent and use method thereof |
| CN106305756A (en) * | 2016-08-25 | 2017-01-11 | 文山苗乡三七科技有限公司 | Formula of pseudo-ginseng seed dressing medicament |
| CN106912490A (en) * | 2017-03-10 | 2017-07-04 | 陕西康禾立丰生物科技药业有限公司 | A kind of efficient bactericidal composite containing tetramycin |
| CN109392929A (en) * | 2017-08-16 | 2019-03-01 | 青岛奥迪斯生物科技有限公司 | A kind of bactericidal composition containing tetramycin and kasugarnycin |
| CN109392931A (en) * | 2017-08-16 | 2019-03-01 | 青岛奥迪斯生物科技有限公司 | A kind of bactericidal composition containing polyoxin and tetramycin |
| CN110250193A (en) * | 2019-08-02 | 2019-09-20 | 江门市植保有限公司 | A kind of composition pesticide containing kasugarnycin and polyoxin |
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| CN110250193A (en) * | 2019-08-02 | 2019-09-20 | 江门市植保有限公司 | A kind of composition pesticide containing kasugarnycin and polyoxin |
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