CN110024804A - A kind of agricultural bactericidal composition containing kasugarnycin and polyoxin - Google Patents
A kind of agricultural bactericidal composition containing kasugarnycin and polyoxin Download PDFInfo
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- CN110024804A CN110024804A CN201910441519.6A CN201910441519A CN110024804A CN 110024804 A CN110024804 A CN 110024804A CN 201910441519 A CN201910441519 A CN 201910441519A CN 110024804 A CN110024804 A CN 110024804A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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Abstract
The invention discloses a kind of agricultural bactericidal composition containing kasugarnycin and polyoxin comprising bactericidal component and building component；The bactericidal component is the composition of kasugarnycin and polyoxin, and by quality ratio, the compound proportion of kasugarnycin and polyoxin is 50:1~1:4；The building component is leguminous plant astragatus complanatus extract, and by percentage to the quality, accounting of the content of the building component in the agricultural bactericidal composition is 10%~40%.The agricultural bactericidal composition can be used for preventing and treating the diseases such as leaf muld of tomato, cucumber downy mildew, pear scab, Alternaria alternate.
Description
Technical field
The invention belongs to technical field of compounded pesticide, are related to a kind of agricultural bactericidal composition, which, which contains, kills
Bacterium active constituent is kasugarnycin and polyoxin and vegetable active synergistic component.The invention further relates to the bactericidal compositions
Preparation formulation and its in application agriculturally.
Background technique
Kasugarnycin, also known as kasugarnycin, kasumin etc., chemical name: (5- amino-2-methyl -6- (2,3,4,5,6-
Hydroxy-cyclohexyl oxo) tetrahydropyran -3-base) amino-α-amine acetic acid is in April, 1963 from Nara County, Japan springtime sacrificial place
Amino glucosides antibiotics caused by a kind of isolated actinomyces in domestic soil have toxicity low, efficient and interior
The advantages that sorptivety is thoroughly strong；Its mechanism of action is to interfere the esterase system of pathogen amino acid metabolism, and the biology for destroying protein closes
At inhibiting the growth of mycelia and cause cell granulations, pathogen is made to lose breeding and infection ability；It is currently used for anti-harness the river
Rice rice blast, cucumber fusarium axysporum, cucumber bacterial angular leaf spot, cucumber downy mildew, cucumber samping off, soft rot of cabbage, Chinese cabbage
Black rot, wildfire, leaf muld of tomato, blakleg of potato, watermelon bacterial angular leaf spot, citrus bacterial canker disease, peach are brown
Spot shothole disease etc..In agricultural production, the dosage form of kasugarnycin mainly has wettable powder, pulvis, aqua.Kasugarnycin can be with
Multiple Pesticides are mixed, usually should not be mixed with Basic pesticides.Kasugarnycin is to tobacco safety.
Polyoxin is metabolite caused by streptomyces aureus, belongs to antibiotics fungicide, is had in preferable
Inhale conduction；Its mechanism of action is to interfere the biosynthesis of germ cell wall chitin, and somatic cells wall is made not can be carried out life
Object synthesizes and causes germ dead, and after germ tube and mycelia contact medicament, local bulkiness, overflows cell inclusion at rupture, and cannot
Normal development leads to death；It is mainly used for preventing and treating alternaria leaf spot of apple, apple tree graywall, cucumber downy mildew, cucumber white powder
Disease, early blight of tomato and late blight, leaf muld of tomato, rice blast, rice seedling damping-off, watermelon blight, wheat are red
Mildew, flowers powdery mildew etc..Polyoxin is to tobacco safety.
Plant disease is the infringement that fungi, bacterium or virus generate plant during the growth process, and influences agricultural life
One of natural calamity of production.However, controlling disease is easiest to problem appear to is that disease is anti-in the production real process of agricultural
The generation of pharmacological property.Different pesticide species are used in compounding, and are to prevent and treat the very common method of resistance disease.Heterogeneity is compounded,
According to practical application effect, to judge that complex composition is adduction, synergy or antagonism.In most cases, pesticide
Compounding effect be all additive effect, really have the compounding of synergistic effect less, especially synergistic effect clearly, altogether poison system
The very high compounding of number is just less.Although kasugarnycin and polyoxin are all antibiotics systemic fungicides, machine is acted on
Manage it is not identical, inventor herein based on the above-mentioned thinking being used in compounding, probed into kasugarnycin and polyoxin compounding to kind
The control efficiency of the diseases such as eggplant leaf mold, cucumber downy mildew, pear scab, Alternaria alternate.But hair is studied by inventor
Existing, kasugarnycin and polyoxin are used in compounding to leaf muld of tomato, cucumber downy mildew, pear scab, Alternaria alternate etc.
The control efficiency of disease is unsatisfactory.
The Main way of botanical pesticide research in recent years concentrates on finding that desinsection, sterilization are lived from Plant Secondary Metabolites
Property ingredient, less focus on from plant searching pesticide synergistic ingredient.The research of plant source synergist is no lack of successful example, than
Such as, sesame oil has good synergistic effect to chrysanthemum ester insecticide；Sophora alopecuroides extract can improve a variety of organophosphorus insecticides
Insecticidal effect.Although plant source synergist itself does not have desinsection, bactericidal activity, killing for chemical pesticide can be greatly improved
Worm, bactericidal activity, to achieve the purpose that reduce chemical pesticide usage amount.Patent application CN108041083A discloses a plant
Material resource pesticide synergistic agent, the component of the synergist include leguminous plant astragatus complanatus extract.This botanical pesticide increases
Application of the agent in terms of chemical pesticide synergy is imitated, to pyrethroid insecticides, organophosphorus insecticide, carbamates
Insecticide, anabasine insecticide, pyrrole insecticides, Insecticidal pyrazolines, a plurality of types of chemistry of pyridines agrochemical
Insecticide has good synergistic effect.
In the prior art, patent application CN104488929A discloses a kind of composition for preventing and treating cucumber fusarium axysporum, component
Including kasugarnycin, polyoxin, propamidine, Zhongshengmycin etc., the composition can efficiently prevent and treat cucumber fusarium axysporum.It needs
, it is noted that not yet finding kasugarnycin and polyoxin in the prior art, there are cross resistances, while having no that leguminous plant is flat
Stem Astragalus Root P.E has the report of synergistic effect to kasugarnycin, polyoxin binary built.
Summary of the invention
The technical problems to be solved by the invention: based on conventional compounding thinking, present patent application inventor is obtained by test
Know, kasugarnycin and polyoxin binary built are to leaf muld of tomato, cucumber downy mildew, pear scab, Alternaria alternate etc.
The control efficiency of disease is unsatisfactory.
With people's environmental protection, the enhancing of social sustainable development consciousness, the side effect of existing pesticide is utmostly reduced, it is real
The reasonable employment of existing chemical pesticide had become the widespread consensus of those skilled in the art already.Using various during the applications of pesticide
Synergist, improve pesticide spraying during utilization rate, improve drug effect, reduce pesticide usage amount be also a kind of practicable
Solution.Based on this Research Thinking, present patent application has inquired into leguminous plant astragatus complanatus extract in antibiotics
The synergistic effect of absorption fungicide provides a kind of agricultural bactericidal composition containing kasugarnycin and polyoxin.
First purpose of present patent application is to solve above-mentioned technical problem, specifically, described containing kasugarnycin and more
The agricultural bactericidal composition of antimycin comprising bactericidal component and building component；The bactericidal component is kasugarnycin and resists more
The composition of mycin, by quality ratio, the compound proportion of kasugarnycin and polyoxin are 50:1~1:4；The building component
For leguminous plant astragatus complanatus extract, by percentage to the quality, the content of the building component is combined in the agricultural bactericidal
Accounting in object is 10%~40%.
Astragatus complanatus (Astragalus complanatus R.Ex Bge.) also known as great Sha aster, Xia Huangcao, are at climing Radix Astragali
Pulse family Astragalus.Its seed is used as medicine, the entitled complanate astragalus seed of medicinal material, has the function of kidney tonifying, controlling nocturnal emission with astringent drugs, reducing urination, nourishes the liver to improve visual acuity, and uses
In treatment dizzy blurred vision, soreness and weakness of waist and knees, enuresis frequent micturition, the heeltap not to the utmost, the diseases such as premature ejaculation of passing out semen.Astragatus complanatus is a kind of widely distributed
Wild herb in China North China, northeast, weinan area as Chinese medicine large area plant, seed be in
Medicine " semen astragali complanati ".Meanwhile astragatus complanatus is also the feed that a kind of ox, sheep are fond of, nutritive value is higher than clover.Harvest seed
Astragatus complanatus stalk, rhizome except on a small quantity as livestock store away in winter feed in addition to, mostly as agricultural wastes.
Leguminous plant astragatus complanatus extract of the present invention can be the root, stem, leaf of astragatus complanatus, can also use flat stem
The whole strain of Radix Astragali.It is solely extracted containing the astragatus complanatus it should be pointed out that the plant source pesticide synergist can be
Object；Or the mixture or composition of the astragatus complanatus extract and auxiliary agent, carrier etc.；Or the astragatus complanatus extract
With the composition of other active constituent.In the present invention, the astragatus complanatus extract is preferably astragatus complanatus whole plant
Extract.In other embodiment, it is preferable that the astragatus complanatus extract with Astragalus complanatus (astragatus complanatus
Seed) be raw material extract.
About the preparation method of the astragatus complanatus extract, the present invention to the preparation method of astragatus complanatus extract not
It is particularly limited.Astragatus complanatus extract provided by the invention for raw material, is passed through with astragatus complanatus plant (including stem, Ye Hegen)
After dried and crushed, it can be obtained by flooding, organic solvent extraction, supercritical fluid extraction or other conventional modes.
Organic solvent extraction can be used as one of the preferred embodiment for obtaining the astragatus complanatus extract, and operating process is big
Cause as follows: the astragatus complanatus after crushing drying extracts gained crushed material with organic solvent, recycles the organic solvent in leaching liquor,
The concentrate of the astragatus complanatus extract is obtained to get the astragatus complanatus extract.Organic solvent of the present invention is preferred
It is one of methanol, ethyl alcohol, chloroform, acetone, ethyl acetate or its mixture or other can obtains the astragatus complanatus
The organic solvent of extract.
Specifically, the method that the application uses organic solvent to extract the astragatus complanatus extract are as follows: after taking dried and crushed
Astragatus complanatus plant in stirring container, be added 4~8 times of weight organic solvent, stirred in 40~60 DEG C of thermostat water baths
Mix extraction 2~4 times, every time 12~36h；Separating and filtering obtains leaching liquor, and leaching liquor is steamed at vacuum degree 0.05Mpa~0.09Mpa
Hair concentration, recycles the organic solvent in leaching liquor, obtains the astragatus complanatus extract.
As one of preferred embodiment, in the agricultural bactericidal composition containing kasugarnycin and polyoxin,
The compound proportion of the kasugarnycin and polyoxin is 5:1；The leguminous plant astragatus complanatus extract agricultural is killed described
Accounting in bacteria composition is 10%~40%.
As one of preferred embodiment, in the agricultural bactericidal composition containing kasugarnycin and polyoxin,
The compound proportion of the kasugarnycin and polyoxin is 1:4；The leguminous plant astragatus complanatus extract agricultural is killed described
Accounting in bacteria composition is 10%~40%.
As one of preferred embodiment, in the agricultural bactericidal composition containing kasugarnycin and polyoxin,
The compound proportion of the kasugarnycin and polyoxin is 50:1；The leguminous plant astragatus complanatus extract agricultural is killed described
Accounting in bacteria composition is 10%~40%.
Second of present patent application is designed to provide comprising the agricultural bactericidal containing kasugarnycin and polyoxin
The pesticide of composition.
The dosage form of the pesticide can be suspending agent, wettable powder or water dispersible granules.Those skilled in the art's reference
The agricultural bactericidal composition containing kasugarnycin and polyoxin is configured to the suspending agent, wettable powder by the prior art
The formulations of pesticide such as agent or water dispersible granules, for use in leaf muld of tomato, cucumber downy mildew, pear scab, Alternaria alternate
Etc. diseases prevention and treatment.When preparing above-mentioned dosage form, it can according to need selection and (helped using different auxiliary ingredients for pesticide preparations
Agent).The auxiliary element be decentralized medium, dispersing agent, emulsifier, wetting agent, thickener, defoaming agent, antifreezing agent, disintegrating agent,
One or more of binder, filler etc..
When preparing suspending agent type pesticide, workable auxiliary agent has: dispersing agent such as polycarboxylate, lignosulfonates, alkane
It is a kind of in base naphthalene sulfonate (dispersing agent NNO), TERSPERSE 2020 (Huntsman Corporation, the U.S. produces, alkyl naphthalene sulfonic acid salt)
Or it is a variety of；Emulsifier such as Nongru-700 # (common name: alkyl phenol formaldehyde resin polyoxyethylene ether), agriculture breast 2201, this disk -60# are (logical
With name: Span60), Tween-60 # (common name: polyoxyethylene sorbitan stearate), agriculture breast
1601# (common name: triphenoethyl benzene phenol polyoxyethylene polyoxypropylene block polymer), (U.S.'s Hensel of TERSPERSE 4894
Step company produce) one of or it is a variety of；Wetting agent such as alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkyl phenol are poly-
Oxyethylene group ether phosphate, phenethyl phenol polyoxyethylene groups ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate,
It is one or more in TERSPERSE 2500 (Huntsman Corporation, U.S. product)；Thickener such as xanthan gum, polyvinyl alcohol, swelling
It is one or more in soil, aluminium-magnesium silicate；It is preservative such as formaldehyde, benzoic acid, one or more in sodium benzoate；Stabilizer such as epoxy
It is soybean oil, epoxychloropropane, one or more in triphenyl phosphate；Defoaming agent such as organic silicon defoaming agent；Antifreezing agent such as second two
It is one or more in alcohol, propylene glycol, glycerol, urea, inorganic salts such as sodium chloride；Water is deionized water.
Using 71.4% kasugarnycin (kacugamycin) raw medicine, the female medicine of 40% polyoxin (polyoxin) as sterilization group
Point, with the ethanol extract building component of leguminous plant astragatus complanatus, providing a kind of includes described containing kasugarnycin and more anti-mildews
The suspending agent type pesticide of the agricultural bactericidal composition of element.Specifically, the component of this suspending agent type pesticide, in terms of mass fraction,
Including kasugarnycin 5%, polyoxin 1%, the ethanol extract 10% of leguminous plant astragatus complanatus, dispersing agent 4~12%, profit
Humectant 1~6%, viscosity modifier 1~3%, antifreezing agent 2~4%, defoaming agent 0.1~0.5%, surplus is supplied with deionized water；
The suspending agent is processed according to the process of ingredient, precomminution, sand mill crushing, mixing preparation, packaging, and wherein sand mill crushing makes
With four sand mills, plural serial stage, using fine sand and coarse sand, all dress coarse sand, second grinding machine fill 2/3 to First grinding machine
Coarse sand and 1/3 fine sand, third platform sand mill fill 1/3 coarse sand and 2/3 fine sand, and the 4th sand mill all fills fine sand.By this system
6% kasugarnycin polyoxin suspending agent is made in Preparation Method.
For water dispersible granule pesticide, those skilled in the art, which are very familiar with, completes this hair using corresponding auxiliary agent
It is bright.Dispersing agent such as polycarboxylate (TERSPERSE 2700, T36, GY-D06), lignosulfonates, in alkylnaphthalene sulfonate
It is one or more；It is wetting agent such as alkyl sulfate, alkylsulfonate, one or more in naphthalene sulfonate；Disintegrating agent such as ammonium sulfate,
It is one or more in sodium sulphate, polyvinylpyrrolidone, starch and its derivative, bentonite；Binder for example starch, glucose,
One of polyvinyl alcohol, polyethylene glycol, sodium carboxymethylcellulose, sucrose are a variety of；Filler such as diatomite, kaolin, hard charcoal
Black, light calcium, talcum powder, attapulgite, clay are one or more.
Using 71.4% kasugarnycin (kacugamycin) raw medicine, the female medicine of 40% polyoxin (polyoxin) as sterilization group
Point, with the ethanol extract building component of leguminous plant astragatus complanatus, providing a kind of includes described containing kasugarnycin and more anti-mildews
The water dispersible granule pesticide of the agricultural bactericidal composition of element.Specifically, the component of this water dispersible granule pesticide, with quality
Number meter, including kasugarnycin 5%, polyoxin 20%, the ethanol extract 25% of leguminous plant astragatus complanatus,
TERSPERSE 2,700 5%, sodium lignin sulfonate 3%, Nekal BX 1%, polyvinylpyrrolidone 4%, ammonium sulfate 6%,
White carbon black 3%, diatomite add to 100% parts by weight.The method i.e. mixing, ultra micro of the frequent regulation water intaking dispersible granule of above-mentioned raw materials
Air-flow crushing, mixing, granulation step produce 25% kasugarnycin polyoxin water dispersible granules.
When preparing wettable powder dosage form pesticide, auxiliary agent workable for those skilled in the art has: dispersing agent such as polycarboxylic acids
Salt (TERSPERSE 2700, T36, GY-D06 etc.), lignin sulfonate (Ufoxane 3A, Borresperse NA,
Borresperse CA-SA etc.), naphthalene and alkyl naphthalene formaldehyde condensate sulfonate class (NNO, MF, Morwet D-425, Tamol
NN, TERSPERSE2020 etc.), Nekal BX (nekal), EO-PO block polyether class, alkylphenol-polyethenoxy base
Ether phosphate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate (SOPA), in it is one or more；Wetting agent such as sulfate
It is class (K-12), Sulfonates (ABS-Na, BX, Terwet 1004 etc.), a kind of or more in compound wetting agent (Morwet EFW)
Kind；Filler such as diatomite, kaolin, light calcium, talcum powder, white carbon black, attapulgite, clay, ammonium sulfate, urea, sucrose, grape
Sugar, cornstarch, sodium sulphate, sodium polyphosphate etc. are one or more.
Using 71.4% kasugarnycin (kacugamycin) raw medicine, the female medicine of 40% polyoxin (polyoxin) as sterilization group
Point, with the ethanol extract building component of leguminous plant astragatus complanatus, providing a kind of includes described containing kasugarnycin and more anti-mildews
The wettable powder dosage form pesticide of the agricultural bactericidal composition of element.Specifically, the component of this wettable powder dosage form pesticide, with quality
Number meter, including kasugarnycin 50%, polyoxin 1%, the ethanol extract 10% of leguminous plant astragatus complanatus, Ufoxane
3A 5%, Morwet D-425 4%, lauryl sodium sulfate 2%, white carbon black 8%, kaolin are filled up to 100% weight
Part.Above-mentioned raw materials are prepared into 51% kasugarnycin polyoxin wettable through mixing, ultra micro air-flow crushing, hybrid technique step
Pulvis.
The third of present patent application is designed to provide described containing the combination of the agricultural bactericidal of kasugarnycin and polyoxin
Object, or the pesticide comprising the agricultural bactericidal composition containing kasugarnycin and polyoxin is in prevention and treatment leaf muld of tomato, Huang
Melon downy mildew, pear scab, the application in Alternaria alternate.
Agricultural bactericidal composition of the present invention containing kasugarnycin and polyoxin, at least has the following beneficial effects
Or advantage.
Though kasugarnycin and polyoxin are antibiotics systemic fungicide, the two has different effect machine
Reason.The farm chemical ingredients of different role mechanism are compounded, are the effective ways for preventing and treating corps diseases resistance and generating.In view of opening
Hair has many advantages, such as that small investment, R&D cycle are short with the compound preparation for studying efficient, low toxicity, low-residual, thus existing active group
The attention being used in compounding by those skilled in the art divided.However based on this conventional thought the study found that kasugarnycin
With polyoxin binary built, prevention and treatment to diseases such as leaf muld of tomato, cucumber downy mildew, pear scab, Alternaria alternates
The effect is unsatisfactory.
Present patent application innovatively filters out leguminous plant astragatus complanatus extract as kasugarnycin and polyoxin
The building component of binary built, accounting of the content of the building component in the agricultural bactericidal composition be 10%~
40%, the compound proportion of kasugarnycin and polyoxin is 50:1~1:4, and within this range, leguminous plant astragatus complanatus is extracted
Object has excellent synergistic effect to kasugarnycin and polyoxin binary built.
Bactericidal component of the present invention is the composition of kasugarnycin and polyoxin, and it is mould on the one hand to efficiently solve spring thunder
Element and polyoxin binary built imitate the disease controls such as leaf muld of tomato, cucumber downy mildew, pear scab, Alternaria alternate
The undesirable technological deficiency of fruit, expands the prevention spectrum of medicament；On the one hand resistance occurrence risk is avoided or reduced, it is mould to extend spring thunder
The service life of element, polyoxin prevention and treatment plant (crop) disease.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated on.It should be appreciated that described herein, specific examples are only used to explain the present invention, is not used to limit
The fixed present invention, any modifications, equivalent replacements and improvementsmade within the spirit and principles of the invention, etc. should be included in
Within protection scope of the present invention.
The method that the present embodiment obtains the astragatus complanatus extract are as follows: the astragatus complanatus after taking 1000g dried and crushed is planted
Strain is added the organic solvent of 4~6 times of weight, stirs in 40~60 DEG C of (50 DEG C or so) thermostat water baths in stirring container
It extracts 2~4 times (usually 3 times), every time 12~36h (preferably 20h)；Separating and filtering obtains leaching liquor, and leaching liquor is in vacuum degree
It is concentrated by evaporation under setting 0.05Mpa~0.06Mpa, recycles the organic solvent in leaching liquor, obtain the astragatus complanatus extract.
Organic solvent described in the present embodiment selects ethyl alcohol.
Embodiment 1
The present embodiment provides medicament to the co-toxicity of leaf muld of tomato, and measurement result is shown in Table 1.
Never the tomato leaf of the infection leaf muld of tomato of pesticide was applied in acquisition greenhouse, again with sterile after clear water flushing
For 24 hours, with conidium under sterile washing, it is spare that spore suspension is made in moisturizing at water rinses one time, 20~25 DEG C.
On the basis of trial test, preparing concentration containing following agents respectively with PSA culture medium (pH=7.0) is 1 μ g/mL's
The malicious plate of band, and set not dosing PSA plate and compare, every processing is repeated 3 times.Wherein, the group of the PSA culture medium becomes potato
200g, sucrose 20g, agar 10g, distilled water 1000ml.100 μ L spore suspensions are added on drug containing PSA plate with pipettor,
Coating uniformly, while setting blank control.It is placed at 24 DEG C after constant temperature incubation 20 hours, observes conidia germination under the microscope
Situation.100 conidiums are investigated in every processing.The half that germ tube length is greater than conidium diameter is considered as sprouting.
The EC of single dose and mixture is calculated using SPSS software50And related coefficient, and calculated according to Wadley (1945) method
Coefficient of synergism SR under different ratio out, the mode of application after medicament mixture is judged with SR value: SR < 0.5 is antagonism work
With；1.5 >=SR >=0.5 is summation action；SR > 1.5 is synergistic effect.
SR=X1/X2, wherein X1 is mixture EC50Theoretical value (mg/L), X2 are mixture EC50Measured value (mg/L).
1 kasugarnycin of table, polyoxin and its mixture are to leaf muld of tomato toxicity test result
Research shows that (table 1), in vitro, kasugarnycin, polyoxin proportion mixture (compound proportion be 50:1~
Do not have stronger inhibiting effect to leaf muld of tomato 1:4), coefficient of synergism (SR) is respectively 1.42,1.44,1.38,1.43,
1.47, it can be seen that kasugarnycin and polyoxin be not in when 50:1~1:4 ratio mixture and appearing as synergistic effect；It mixes herein
With in proportional region, astragatus complanatus extract 10~40% is added, coefficient of synergism (SR) has different degrees of promotion, is all larger than
1.5, show as synergistic effect；And kasugarnycin, polyoxin mixed ratio when being 50:1 coefficient of synergism highest, no antagonism make
With.
Embodiment 2
The present embodiment provides medicament to the co-toxicity of Alternaria alternate, and measurement result is shown in Table 2.Tobacco brown spot pathogen is by west
Northern agriculture and forestry science and technology university Plant Pathology laboratory provides.
Using the plate mycelia growth inhibition assay of NY/T1156.6-2006, on the basis of trial test, with PSA culture medium
(pH=7.0) the malicious plate of band that concentration containing following agents is 1 μ g/mL respectively is prepared, and sets not dosing PSA plate and compares.Its
In, the composition of the PSA culture medium is the same as embodiment 1.The sterilization punchers for using diameter 5mm under aseptic technique, from bacterium colony
Edge cuts bacteria cake, and with inoculator by pure culture biscuits involvng inoculation in the PSA plate center of drug containing, mycelia is face-up, covers ware lid, is placed in 24
Constant temperature incubation 48 hours at DEG C, with calliper to measure colony diameter, unit mm, each bacterium colony is straight with crossing method vertical measurement
Diameter is each primary, takes its average value.
The EC of single dose and mixture is calculated using SPSS software50And related coefficient, and calculated according to Wadley (1945) method
Coefficient of synergism SR under different ratio out, the mode of application after medicament mixture is judged with SR value: SR < 0.5 is antagonism work
With；1.5 >=SR >=0.5 is summation action；SR > 1.5 is synergistic effect.
SR=X1/X2, wherein X1 is mixture EC50Theoretical value (mg/L), X2 are mixture EC50Measured value (mg/L).
2 kasugarnycin of table, polyoxin and its mixture are to Alternaria alternate toxicity test result
Research shows that (table 2), in vitro, kasugarnycin, polyoxin proportion mixture (compound proportion be 50:1~
1:4) there is different degrees of inhibiting effect to Alternaria alternate, when the two proportion is 2:1 and 3:1, coefficient of synergism (SR) is respectively
It is 1.75,1.67, is all larger than 1.5, shows as synergistic effect；When the two proportion is 5:1,4:1,50:1, coefficient of synergism (SR) point
Not Wei 1.49,1.44 and 1.41, respectively less than 1.5, show as summation action；However, astragatus complanatus extract 10~40% is added,
Coefficient of synergism (SR) has different degrees of promotion, is all larger than 1.5, shows as synergistic effect.This illustrates that adding astragatus complanatus mentions
Object is taken, has been obviously improved kasugarnycin, polyoxin matches mixture to the inhibiting effect of Alternaria alternate.
Embodiment 3
The present embodiment provides medicament to the field control effect of Alternaria alternate, and measurement result is shown in Table 3.Test is in Yunnan Province
Carry out to the tobacco of Chuxiong.Test sets 16 processing altogether, is control with clear water, and test is administered 2 times altogether, is spaced 10 days, by medicine
Liquid uniformly sprays application to tobacco complete stool.
Investigation method: 5 points of samplings of each processing, every 2 plants of random searching, the whole blades of every plant of investigation record total number of sheets
And each sick both grade numbers of sheets, it is classified according to following grade scale, is administered observation in 10 days after the application of preceding and last next time.
Sick leaf stage division:
0 grade: disease-free spot；
1 grade: lesion area accounts for 1% or less entire leaf area；
3 grades: lesion area accounts for the 2%-5% of entire leaf area；
5 grades: lesion area accounts for the 6%-10% of entire leaf area；
7 grades: lesion area accounts for the 11%-20% of entire leaf area；
9 grades: lesion area accounts for 21% or more of entire leaf area.
Disease index and control efficiency calculation formula are as follows:
The field control effect of 3 kasugarnycin of table, polyoxin and its mixture to Alternaria alternate
From table 3 it can be seen that kasugarnycin, polyoxin proportion mixture are to Alternaria alternate under the conditions of field trial
Field efficacy synergistic effect be not obvious；But after adding astragatus complanatus extract, kasugarnycin, polyoxin match mixture
There is different degrees of promotion to the field efficacy of Alternaria alternate, 10 days preventive effects may remain in 70% after the 1st application
More than, 10 days preventive effects may remain in 75% or more after the 2nd application.
Embodiment 4
The present embodiment provides medicament to the field control effect of pear scab, and measurement result is shown in Table 4 and table 5.Test exists
Carry out to the crisp pears of Anhui Province's Dangshan County.Test sets 16 processing altogether, is control with clear water, and test is administered 2 times altogether, interval 10
Medical fluid, is uniformly sprayed application to pear tree complete stool by it.
Investigation method: each processing randomly chooses 2 trees, each tree according to east, south, west, north, in 5 points of samplings, often click
5 leaves and 5 fruits are selected, it is each to handle total 50 leaves and 50 fruits, it is classified according to following grade scale, next time applies
Before medicine and 10 days after last application, total leaf (fruit) number, sick leaf (fruit) number are recorded, diseased plant rate, disease index and preventive effect are calculated.
Sick leaf stage division:
0 grade: disease-free spot；
1 grade: lesion area accounts for 10% or less entire leaf area；
3 grades: lesion area accounts for the 11%-25% of entire leaf area；
5 grades: lesion area accounts for the 26%-40% of entire leaf area；
7 grades: lesion area accounts for the 41%-65% of entire leaf area；
9 grades: lesion area accounts for 65% or more of entire leaf area.
Disease fruit stage division:
0 grade: disease-free spot on fruit；
1 grade: having scab 1-2 on each fruit；
3 grades: having scab 3-4 on each fruit；
5 grades: having scab 5-6 on each fruit；
7 grades: having scab 7-10 on each fruit, part scab, which is connected, accounts for fruit area 1/5 or so；
9 grades: having scab 10 or more on each fruit, part scab, which is connected, accounts for 1/4 or more fruit area.
Disease index and control efficiency calculation formula are as follows:
The field control effect (blade) of 4 kasugarnycin of table, polyoxin and its mixture to pear scab
From table 4, it can be seen that kasugarnycin, polyoxin proportion mixture are to pear scab under the conditions of field trial
The field efficacy synergistic effect of blade is not obvious；But after adding astragatus complanatus extract, kasugarnycin, polyoxin proportion
Mixture has different degrees of promotion to the field efficacy of pear scab blade, and 10 days preventive effects can protect after the 1st application
Holding 10 days after 60% or more, the 2nd application preventive effects may remain in 70% or more.
The field control effect (fruit) of 5 kasugarnycin of table, polyoxin and its mixture to pear scab
As can be seen from Table 5, under the conditions of field trial, kasugarnycin, polyoxin proportion mixture are to pear scab
The field efficacy synergistic effect of fruit is not obvious；But after adding astragatus complanatus extract, kasugarnycin, polyoxin proportion
Mixture has different degrees of promotion to the field efficacy of pear scab fruit, 10 days preventive effects and the 2nd after the 1st application
10 days preventive effects may remain in 70% or more after secondary application.
Embodiment 5
The present embodiment provides medicament to the field control effect of cucumber downy mildew, and measurement result is shown in Table 6.Test is in Henan Province
Carry out to the plastic tent cucumber of Anyang Neihuang County.Test sets 16 processing altogether, is control with clear water, and test is administered 2 times altogether, interval
10 days, medical fluid is uniformly sprayed application to cucumber complete stool.
Investigation method: each processing randomly chooses 5 points, and 5 leaves of every fixed selection amount to 25 leaves, according to
Cucumber downy mildew grade scale is classified, and before application next time and 10 days after last application, is recorded total strain number, diseased plant number, is calculated disease
Strain rate, disease index and preventive effect.
Stage division:
0 grade: disease-free spot；
1 grade: lesion area accounts for 5% or less entire leaf area；
3 grades: lesion area accounts for the 6%-10% of entire leaf area；
5 grades: lesion area accounts for the 11%-25% of entire leaf area；
7 grades: lesion area accounts for the 26%-50% of entire leaf area；
9 grades: lesion area accounts for 50% or more of entire leaf area.
Disease index and control efficiency calculation formula are as follows:
The field control effect of 6 kasugarnycin of table, polyoxin and its mixture to cucumber downy mildew
As can be seen from Table 6, under the conditions of field trial, kasugarnycin, polyoxin proportion mixture are to cucumber downy mildew
Field efficacy synergistic effect be not obvious；But after adding astragatus complanatus extract, kasugarnycin, polyoxin match mixture
There is different degrees of promotion to the field efficacy of cucumber downy mildew, 10 days preventive effects may remain in 62% after the 1st application
More than, 10 days preventive effects may remain in 72% or more after the 2nd application.
Claims (8)
1. a kind of agricultural bactericidal composition containing kasugarnycin and polyoxin, which is characterized in that including bactericidal component and synergy
Component；The bactericidal component is the composition of kasugarnycin and polyoxin, by quality ratio, kasugarnycin and polyoxin
Compound proportion is 50:1~1:4；The building component is leguminous plant astragatus complanatus extract, by percentage to the quality, described
Accounting of the content of building component in the agricultural bactericidal composition is 10%~40%.
2. the agricultural bactericidal composition containing kasugarnycin and polyoxin according to claim 1, which is characterized in that the spring
The compound proportion of thunder mycin and polyoxin is 5:1；Astragatus complanatus extract the accounting in the agricultural bactericidal composition
Than being 10%~40%.
3. the agricultural bactericidal composition containing kasugarnycin and polyoxin according to claim 1, which is characterized in that the spring
The compound proportion of thunder mycin and polyoxin is 1:4；Astragatus complanatus extract the accounting in the agricultural bactericidal composition
Than being 10%~40%.
4. the agricultural bactericidal composition containing kasugarnycin and polyoxin according to claim 1, which is characterized in that the spring
The compound proportion of thunder mycin and polyoxin is 50:1；Astragatus complanatus extract the accounting in the agricultural bactericidal composition
Than being 10%~40%.
5. according to claim 1 to the agricultural bactericidal composition described in any one of 4 containing kasugarnycin and polyoxin, feature
Be, the astragatus complanatus extract the preparation method comprises the following steps: the astragatus complanatus plant after taking dried and crushed adds in stirring container
The organic solvent for entering 4~8 times of weight, the stirring and leaching 2~4 times in 40~60 DEG C of thermostat water baths, every time 12~36h；Separation
Leaching liquor is filtered to obtain, leaching liquor is concentrated by evaporation at vacuum degree 0.05Mpa~0.09Mpa, the organic solvent in leaching liquor is recycled,
Obtain the astragatus complanatus extract；The organic solvent be selected as one of methanol, ethyl alcohol, chloroform, acetone, ethyl acetate or
It is its mixture.
6. the pesticide comprising agricultural bactericidal composition described in any one of claims 1 to 4.
7. agricultural bactericidal composition described in any one of claims 1 to 4 is in prevention and treatment leaf muld of tomato, cucumber downy mildew, pear tree
Application in scab, Alternaria alternate.
8. pesticide described in claim 6 is in prevention and treatment leaf muld of tomato, cucumber downy mildew, pear scab, Alternaria alternate
Using.
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| CN110250172A (en) * | 2019-08-05 | 2019-09-20 | 四川百事东旺生物科技有限公司 | A kind of bactericidal composition comprising kasugarnycin and polyoxin and its application |
| CN110250193B (en) * | 2019-08-02 | 2021-08-24 | 江门市植保有限公司 | Pesticide composition containing kasugamycin and polyoxin |
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