CN110225971A - 一组催化糖链延伸的udp-糖基转移酶及其应用 - Google Patents
一组催化糖链延伸的udp-糖基转移酶及其应用 Download PDFInfo
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- CN110225971A CN110225971A CN201880006906.0A CN201880006906A CN110225971A CN 110225971 A CN110225971 A CN 110225971A CN 201880006906 A CN201880006906 A CN 201880006906A CN 110225971 A CN110225971 A CN 110225971A
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Abstract
本发明涉及一组糖基转移酶及其应用。具体地，提供了糖基转移酶GT29‑32、GT29‑33、GT29‑34、GT29‑4、GT29‑5、GT29‑7、GT29‑9、GT29‑11、GT29‑13、GT29‑17、GT29‑18、GT29‑19、GT29‑20、GT29‑21、GT29‑22、GT29‑23、GT29‑24、GT29‑25、GT29‑36、GT29‑37、GT29‑42、GT29‑43、GT29‑45、GT29‑46、PNUGT29‑1、PNUGT29‑2、PNUGT29‑3、PNUGT29‑4、PNUGT29‑5、PNUGT29‑6、PNUGT29‑7、PNUGT29‑8、PNUGT29‑9、PNUGT29‑14和PNUGT29‑15及其衍生多肽在四环三萜类化合物底物C‑20位第一个糖基、C‑6位第一个糖基上和C‑3位的第一个糖基进行催化从而延伸糖链，以便获得人参皂苷Rg3、人参皂苷Rd、人参皂苷Rb1、人参皂苷Rb3、皂苷DMGG、皂苷DMGX、绞股蓝皂苷LXXV、绞股蓝皂苷XVII、绞股蓝皂苷XIII、绞股蓝皂苷IX、三七皂苷U和、三七皂苷R1、三七皂苷R2、三七皂苷R3、3‑O‑β‑(D‑xylopyranosyl)‑β‑(D‑glucopyranosyl)‑PPD；3‑O‑β‑(D‑xylopyranosyl)‑β‑(D‑glucopyranosyl)‑CK、20‑O‑Glucosylginsenoside Rf和Ginsenoside F3。等人参皂苷产物的催化反应。本发明糖基转移酶还可应用于构建人工合成人参皂苷及多种新人参皂苷及其衍生物。
Description
PCT国内申请，说明书已公开。
Claims (10)
- PCT国内申请，权利要求书已公开。
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| CN201710359069.7A CN108949711A (zh) | 2017-05-19 | 2017-05-19 | 一组催化糖链延伸的udp-糖基转移酶及其应用 |
| PCT/CN2018/087678 WO2018210349A1 (zh) | 2017-05-19 | 2018-05-21 | 一组催化糖链延伸的udp-糖基转移酶及其应用 |
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| CN201880006906.0A Active CN110225971B (zh) | 2017-05-19 | 2018-05-21 | 一组催化糖链延伸的udp-糖基转移酶及其应用 |
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| US (2) | US11542484B2 (zh) |
| JP (2) | JP7504594B2 (zh) |
| KR (1) | KR102338008B1 (zh) |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112831481A (zh) * | 2019-11-22 | 2021-05-25 | 中国科学院分子植物科学卓越创新中心 | 糖基转移酶以及催化糖链延伸的方法 |
| CN112852763A (zh) * | 2020-02-17 | 2021-05-28 | 中国科学院天津工业生物技术研究所 | Pn3-32-i5蛋白及其编码基因在生产三七皂苷R1中的应用 |
| CN114703159A (zh) * | 2022-03-15 | 2022-07-05 | 华南理工大学 | 一种葡糖基转移酶突变体及其应用 |
| CN116286894A (zh) * | 2023-02-22 | 2023-06-23 | 首都医科大学 | 一种催化稀有人参皂苷生物合成的糖基转移酶及其编码基因与应用 |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110592040A (zh) * | 2019-09-18 | 2019-12-20 | 江苏施宇甜生物科技有限公司 | 一种用于生产upd糖基转移酶的重组枯草芽孢杆菌的工艺 |
| CN113444703B (zh) * | 2020-03-26 | 2023-09-01 | 生合万物(苏州)生物科技有限公司 | 催化糖链延伸的糖基转移酶突变体及其应用 |
| CN115109787B (zh) * | 2021-03-17 | 2023-07-14 | 广西科学院 | 一组糖基转移酶基因及其在制备三七/人参皂苷中的应用 |
| CN113088502B (zh) * | 2021-04-25 | 2023-05-09 | 武汉轻工大学 | 一种珠子参的糖基化转移酶基因及其应用 |
| CN114807211B (zh) * | 2022-05-13 | 2023-06-27 | 天津大学 | 代谢甘油生产人参皂苷ck的重组酿酒酵母菌及构建方法 |
| CN116396876B (zh) * | 2022-11-17 | 2024-06-21 | 云南农业大学 | 一种生产人参皂苷Rd的酿酒酵母工程菌及其构建方法 |
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| CN112831481A (zh) * | 2019-11-22 | 2021-05-25 | 中国科学院分子植物科学卓越创新中心 | 糖基转移酶以及催化糖链延伸的方法 |
| CN112831481B (zh) * | 2019-11-22 | 2024-01-19 | 生合万物(上海)生物科技有限公司 | 糖基转移酶以及催化糖链延伸的方法 |
| CN112852763A (zh) * | 2020-02-17 | 2021-05-28 | 中国科学院天津工业生物技术研究所 | Pn3-32-i5蛋白及其编码基因在生产三七皂苷R1中的应用 |
| CN112852763B (zh) * | 2020-02-17 | 2022-05-17 | 中国科学院天津工业生物技术研究所 | Pn3-32-i5蛋白及其编码基因在生产三七皂苷R1中的应用 |
| CN114703159A (zh) * | 2022-03-15 | 2022-07-05 | 华南理工大学 | 一种葡糖基转移酶突变体及其应用 |
| CN114703159B (zh) * | 2022-03-15 | 2023-05-26 | 林影 | 一种葡糖基转移酶突变体及其应用 |
| CN116286894A (zh) * | 2023-02-22 | 2023-06-23 | 首都医科大学 | 一种催化稀有人参皂苷生物合成的糖基转移酶及其编码基因与应用 |
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| JP7504594B2 (ja) | 2024-06-24 |
| US20210163901A1 (en) | 2021-06-03 |
| JP2020520656A (ja) | 2020-07-16 |
| US11542484B2 (en) | 2023-01-03 |
| JP2022137053A (ja) | 2022-09-21 |
| CN110225971B (zh) | 2022-09-13 |
| KR102338008B1 (ko) | 2021-12-29 |
| WO2018210349A1 (zh) | 2018-11-22 |
| CN108949711A (zh) | 2018-12-07 |
| JP7504946B2 (ja) | 2024-06-24 |
| KR20200019146A (ko) | 2020-02-21 |
| US20230203458A1 (en) | 2023-06-29 |
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