CN110201044A - Chinese rose extract, containing its product and its preparation method and application - Google Patents
Chinese rose extract, containing its product and its preparation method and application Download PDFInfo
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- CN110201044A CN110201044A CN201910640014.2A CN201910640014A CN110201044A CN 110201044 A CN110201044 A CN 110201044A CN 201910640014 A CN201910640014 A CN 201910640014A CN 110201044 A CN110201044 A CN 110201044A
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- chlosma
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/738—Rosa (rose)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/06—Antipsoriatics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/08—Antiseborrheics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention discloses a kind of Chinese rose extract, containing its product and its preparation method and application.The preparation method of the Chinese rose extract obtains extracting solution the following steps are included: (1) extracts China rose, wherein solvent is 50~90% ethanol waters, and the mass ratio of China rose and solvent is 1:(6~15), Extracting temperature is 50~70 DEG C；(2) extracting solution is filtered, filtrate is obtained；(3) it is eluted after macroporous resin adsorption filtrate, obtains eluent；(4) after concentrate eluant dry to get.Materials are convenient, and preparation method is simple, Chinese rose extract obtained and can be used for skin disease caused by treating chlosma containing its product, have a extensive future.
Description
Technical field
The present invention relates to a kind of Chinese rose extract, containing its product and its preparation method and application.
Background technique
Chlosma is the resident flora on the mankind and warm-blooded animal skin, belongs to thermophilic lipid Basidiomycetes saccharomyces.It is early
Decades in last century instead of before, people have had found three kinds of chlosmas on warm-blooded animal skin, they are respectively as follows: chaff
Not plump chlosma, M.pachy dermats and sympodium chlosma.Then in the research of people, discovery chlosma shares 7 kinds,
There are also Malassezia cilobosa, obtuse chlosma, restricted chlosma and Si Luofei chlosmas i.e. in addition to above-mentioned 3 kinds.Horse
It draws color bacterium to be mainly grown in the horny layer of epidermis of the mankind, is a kind of flora of thermophilic lipid, mainly with three acyl groups of skin surface
Glycerol and free fatty acid are nutrition, and influence triacylglycerol and free fatty acid secretion is less and reduce.Chlosma
It is grown, is mutually grown on normal human skin with yeast, 7 kinds of chlosma kind Malassezia furfurs can be single with yeast phase and Hyphal form
It is solely grown by intermediary of amino acid, other six kinds then without this characteristic.Chlosma belongs to conditionity pathogenic bacteria, can cause a variety of
Disease, especially skin disease.Therefore, there is more and more extensive research to its pathogenic factor in recent years.A large number of studies show that
Skin disease caused by chlosma is there are many combined factors effect as a result, the density for betiding the bacterium of disease, strain
And metabolite has substantial connection.A large number of studies show that chlosma can cause dandruff, seborrhea, idiocrasy
Dermatitis and psoriasis etc..
Dandruff is usually visually difficult to the scales of skin that peel off observed, and existence form is usually the unicellular formation of keratinization, but is worked as
A certain amount of keratinization is unicellular when sticking together, can clinically observe the white scales of sheet.Behrman is
The scholar of one research dandruff, he summarizes a large amount of data, dandruff is divided into amphitypy by clinical manifestation: pityriasis simplex type
With seborrheica pityriasis type.Pityriasis simplex type is the dandruff for appearing in the greyish white loose drying on normal scalp；Seborrheica pityriasis type
It is more then to show as the scales of skin that peel off, and it is greasy easily stick, waxy crust can be formed, and ooze out with a small amount of stickiness.The angle of normal scalp
The 25-30 layer keratinocyte that matter layer contains complete keratinization and is mutually close to, and in the scalp cuticula of dandruff usually
Less than 10 layers keratinocyte, and the frequent angling insufficiency of these keratinocytes, malalignment, iuntercellular have the gap.
The generation process of dandruff is likely to start from discontinuous, the telangiectasis in papilla area.Neutrophil leucocyte and
Monocyte emigration enters mamillary region, epidermis, causes edema between epidermis, and part meronecrosis forms phlysis, and subsequent
Granulosa disappears, and parakeratosis occurs.In the near future, oedema mitigates, and inflammatory cell fades away.Epidermis self-healing, cuticula
Surface forms the scales of skin that peel off.
Most important microorganism relevant to the dandruff cause of disease is chlosma, has a large amount of clinical and laboratory research
Related theory.It is dispersed with multiple-microorganism on normal scalp, density is about 103-105/mm2, including grape ball
Bacterium, Propionibacterium and chlosma.Chlosma in the case where nutritional condition difference, shows as yeast phase or mycelia in vivo and in vitro
Phase, growth have stringent lipid demand.The different subtype of chlosma on a molecular scale can cause clinical and pathologically
Different inflammatory reactions and a variety of diseases, and Hyphal form and certain yeast can mutually cause a disease.Early in the 1960s just
Someone is substantially better than bacterial inhibitor using the curative effect of fungistat by test expenditure, treatment of dandruff.Recently there are many
Report, take orally or the antifungal drugs such as topical ketoconazole and zinc pyrithione can effective treatment of dandruff it is excessive.
Seborrhea is a kind of chronic skin inflammation occurred on the basis of seborrhea.Clinically with Head And Face and on
Back yellowish red color patch is characterized rice and with apparent inflammatory reaction with greasy scales and incrustation.Skin surface sebum increases
And the change of chemical component, it is a large amount of abnormality proliferations of normal flora for being present in skin, invades skin so as to cause morbidity.From
It is related with the morbidity of seborrhea that Malassez in 1874 is put forward for the first time chlosma, chlosma and seborrhea relationship
Always exist dispute.Heng et al. confirms that recession and the decline of chlosma content of skin lesion are positively correlated.Antifungal is for controlling
After treating seborrhea, chlosma quantity is significantly reduced, and skin lesion is improved, this gives the pass of chlosma and seborrhea
System provides strong evidence.Pechere et al. is the study found that the pathogenic of chlosma is not dependent on its on the skin close
Degree, but determined by chlosma hypotype.Tajima et al. with round pcr analysis shows that, the Main Bacteria on patient skin
Kind is Malassezia cilobosa and restricted chlosma；Also, seborrhea and the specific subtype of restricted chlosma have
It closes.For atopic dermatitis with itch, erythema, furfur, pachyderma is main feature.It is apt to occur in infant, young man and puberty
Women afterwards, it is especially common in neck surface.Inherent cause also plays an important role wherein.Domestic and international some documents show idiocrasy
IgE level increases in dermatitis patients serum.A large number of studies show that atopic dermatitis patients chlosma specific IgE antibody sun
Property rate be 20%-100%, chlosma be atopic dermatitis one of the cause of disease.Antifungal is used it is contemplated that adding, but clinical application
When need as the case may be depending on.
Psoriasis is mainly shown as inflammatory red papules, nanoparticle to mung bean size, after be gradually fused to reddish brown color spot mound
Rash and patch, clear border, surrounding inflammatory infiltration, surface cover the dry silvery white scales of skin that peel off of multilayer.Foreign countries once reported to inactivate
The chaff Pi pityrosporion ovale of existence do patch experiment, Pigs with Psoriasis can be caused in people and rabbit body surface.Also studies have found that silver
There are a kind of anti-chaff Pi chlosma antibody in bits patient's serum, and the soluble component (protein) of chaff Pi chlosma is right
The polymorphonuclear neutrophils of psoriatic have apparent chemotaxis, prompt chaff Pi chlosma that may participate in psoriasis
Isomorphic effect.
Chinese rose (Rosa chinensis Jacq.) is rosaceous plant, and dry flower is the traditional Chinese medicine in China.Closely
With the development of modern medicine over year, discovery Chinese rose extract has the pharmacological actions such as anti-inflammatory, anti-oxidant, antitumor, promoting blood circulation.But
Currently, there is not yet Chinese rose extract can inhibit the report of chlosma.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of preparation method of Chinese rose extract, Chinese rose obtained is extracted
Object can inhibit chlosma.
To achieve the goals above, the present invention adopts the following technical scheme:
The present invention provides a kind of preparation methods of Chinese rose extract comprising following steps:
(1) it extracts China rose and obtains extracting solution, wherein solvent is 50~90% ethanol waters, the matter of China rose and solvent
Amount is than being 1:(6~15), Extracting temperature is 50~70 DEG C；
(2) extracting solution is filtered, filtrate is obtained；
(3) it is eluted after macroporous resin adsorption filtrate, obtains eluent；
(4) after concentrate eluant dry to get.
In the present invention, the China rose is the dry flower of rosaceous plant Chinese rose (Rosa chinensis Jacq.).Institute
Stating 50~90% ethanol waters indicates that the volume fraction of ethyl alcohol in ethanol water is 50~90%, other and so on.
In step (1), it is the preferred solvents 60~80% ethanol waters, is more preferably 70% ethanol water.
In step (1), the mass ratio of the China rose and solvent is preferably 1:(8~12), it is more preferably 1:10.
In step (1), the Extracting temperature is preferably 55~65 DEG C, is more preferably 60 DEG C.
In step (1), the time of the extraction can be 1~3h, preferably 2h.
In step (1), this field general extraction methods are can be used in the extraction, are preferably decocted or are recycled diafiltration, more
It is goodly circulation diafiltration.
It is described to filter the filter method that this field routine can be used in step (2), 80~200 mesh screens are preferably used,
It is more preferably 100 mesh.
It preferably further include that will be filtered again after the filtrate flocculation sedimentation in step (2).The flocculation sedimentation it is preferable
Operating method are as follows: flocculant is added in Xiang Suoshu filtrate, is stood after stirring.Wherein, the flocculant can be commonly used in the art
Flocculant, such as chitosan, polyacrylamide or alum, preferably chitosan.The additive amount of the flocculant can be 0.01
~10%, preferably 0.01~0.05% perhaps 0.05~0.1% perhaps 0.1~0.5% perhaps 0.5~1% or 1
~5% or 5~10%, the percentage is the mass percent that the flocculant accounts for the filtrate.The temperature of the stirring
Preferably 30~50 DEG C, be more preferably 45 DEG C.The time of the stirring is preferably 5~30min, is more preferably 10min.Institute
The time for stating standing is preferably 10~60min, is more preferably 30min.
In step (3), the macroreticular resin can be the macroreticular resin of this field routine model, preferably nonpolar macroporous
Resin or low pole macroreticular resin, such as HP20 macroreticular resin, D101 macroreticular resin or AB-8 macroreticular resin.
The HP20 macroreticular resin is purchased from resin Science and Technology Ltd., Tianjin Nankai university, and the D101 macroreticular resin can
Purchased from Shanghai Huazhen Science and Technology Co., Ltd., the AB-8 macroreticular resin is purchased from Mitsubishi Corporation of Japan.
In step (3), the eluant, eluent of the elution can be 50~90% ethanol waters, preferably 60~80% ethyl alcohol
Aqueous solution is more preferably 70% ethanol water.
In step (4), reduced pressure or normal pressure concentration commonly used in the art is can be used in the concentration.
In step (4), the drying can be used the drying means of this field routine, for example, spray drying, vacuum drying or
Freeze-drying, is preferably dried in vacuo.
The present invention also provides Chinese rose extracts made from a kind of preparation method of Chinese rose extract.
The present invention also provides a kind of products, and it includes the Chinese rose extracts.
In the present invention, the mass fraction that the Chinese rose extract accounts for the product can be 0.0001-50%, preferably
0.01-5% or 10~25% is more preferably 0.2~0.5%.
In the present invention, the product is preferably external preparation or chemical product.
Wherein, the dosage form of this field routine can be used in the external preparation, and preferable dosage form is spray, ointment or cream
Cream.The method preparation of this field routine can be used in the external preparation.The chemical product can be shampoo, hair conditioner, cream, wash
Hand liquid, shower cream or lotion.The method preparation of this field routine can be used in the chemical product.
In the present invention, the usage mode of the external preparation and/or chemical product is generally external preparation for skin.
In the drug of skin disease caused by treating chlosma in preparation the present invention also provides the Chinese rose extract
Application.
In the present invention, skin disease caused by the chlosma is generally that dandruff caused by chlosma is excessive, spy answers
Property dermatitis, seborrhea and psoriasis, the dandruff excessively includes pityriasis simplex type and seborrheica pityriasis type.
In the present invention, the chlosma can be Malassezia furfur, M.pachy dermats, sympodium chlosma, spherical shape
One of chlosma, obtuse chlosma, restricted chlosma and Si Luofei chlosma are a variety of.
On the basis of common knowledge of the art, above-mentioned each optimum condition, can any combination to get each preferable reality of the present invention
Example.
The reagents and materials used in the present invention are commercially available.
The positive effect of the present invention is that:
It is convenient that Chinese rose extract of the invention is drawn materials, and preparation method is simple, can be made into the products such as external preparation or chemical product,
For treating skin disease caused by chlosma, have a extensive future.
Specific embodiment
The present invention is further illustrated below by the mode of embodiment, but does not therefore limit the present invention to the reality
It applies among a range.In the following examples, the experimental methods for specific conditions are not specified, according to conventional methods and conditions, or according to quotient
The selection of product specification.
Reagent used in following embodiment and comparative example is conventional commercial product, and wherein D101 macroreticular resin is purchased from upper
Hai Huazhen Science and Technology Ltd..
Embodiment 1
The preparation of Chinese rose extract
(1) 70% ethanol water 10kg is added into China rose 1kg, 60 DEG C of circulation diafiltrations extract 2h, obtain extracting solution；
(2) 100 mesh net filtration extracting solutions, obtain filtrate, and 1% chitosan solution 500g (solvent in chitosan solution is added
For 2% aqueous acetic acid), 40 DEG C of stirring 10min stand 30min, are filtered until clear again；
(3) clarified solution is poured into D101 macroreticular resin to adsorb, is eluted using 70% ethanol water as eluant, eluent,
Collect eluent；
(4) eluent is concentrated under reduced pressure, after removing second alcohol and water, by being dried in vacuo to get Chinese rose extract.
Embodiment 2
The preparation of Chinese rose extract
50% ethanol water 6kg is added into China rose 1kg, 50 DEG C of circulation diafiltrations extract 2h, obtain extracting solution；Remaining step
Suddenly with embodiment 1.
Embodiment 3
The preparation of Chinese rose extract
90% ethanol water 15kg is added into China rose 1kg, 70 DEG C of circulation diafiltrations extract 2h, obtain extracting solution；Remaining
Step is the same as embodiment 1.
Comparative example 1
The preparation of Chinese rose extract
40% ethanol water 5kg is added into China rose 1kg, 40 DEG C of circulation diafiltrations extract 2h, obtain extracting solution；Remaining step
Suddenly with embodiment 1.
Comparative example 2
The preparation of Chinese rose extract
95% ethanol water 16kg is added into China rose 1kg, 50 DEG C of circulation diafiltrations extract 2h, obtain extracting solution；Remaining
Step is the same as embodiment 1.
Comparative example 3
The preparation of Chinese rose extract
50% ethanol water 17kg is added into China rose 1kg, 75 DEG C of circulation diafiltrations extract 2h, obtain extracting solution；Remaining
Step is the same as embodiment 1.
Application Example 1
The preparation of hair conditioner
Each raw materials and consumption is shown in Table 1, and wherein Chinese rose extract is the preparation gained of embodiment 1.
Preparation process: A phase is warming up to 80~85 DEG C, is stirred evenly.B phase is warming up to 80~85 DEG C, is stirred evenly.It will
A is added in B phase, homogeneous 3min.Cooling stirring.40 DEG C are cooled to hereinafter, addition C phase, stirs evenly to get hair conditioner.
Application Example 2
The preparation of shampoo
Each raw materials and consumption is shown in Table 2, and wherein Chinese rose extract is the preparation gained of embodiment 1.
Table 2
Preparation process:
(1) A phase deionized water, anhydrous citric acid are first added in emulsion pot (main cooker), are slowly added in quick stirring
Carbopol 980 then heats to 80~85 DEG C；The RSAW ESB70/ZA in B phase is continuously added, hydrogen is added after being completely dissolved
Sodium oxide molybdena and methylparaben, cooling after stirring completely.
(2) deionized water and citric acid in C phase are added in kettle, is slowly added to N-hance under fast stirring
3196,80~85 DEG C are then heated to, it is stand-by to be cooled to low temperature after being completely dissolved.
(3) D phase is added when main cooker is cooled to 50~60 DEG C, stirs evenly；E is added when main cooker is cooled to 40~45 DEG C
It mutually with C phase Material, is slowly stirred, controls bubble.
(4) its pH value and viscosity are surveyed in sampling, are cooled to 35 DEG C after qualified, 100 mesh filtering and dischargings are to get shampoo.
Application Example 3
The preparation of spray
Chinese rose extract 0.5%, ethyl alcohol 20%, (70%) sorbierite 10%, glycerol 5%, PH-60 1.5%, NaF
0.03%, essence 1.0%, saccharin 0.1%, sodium benzoate 0.1%, appropriate citric acid, deionized water surplus.
Preparation process: deionized water is heated to 85 DEG C, keeps the temperature 30 minutes.60~70 DEG C are cooled to, it is each to sequentially add remaining
Ingredient stirs evenly.40 DEG C are cooled to hereinafter, up to spray.
Application Example 4
The preparation of shower cream
Each raw materials and consumption is shown in Table 3, and wherein Chinese rose extract is the preparation gained of embodiment 1.
Table 3
Preparation process: deionized water is heated to 85 DEG C, and A phase each component is sequentially added, is stirred evenly.It is cooled to 60~70
DEG C, B phase is added, stirs evenly.40 DEG C are cooled to hereinafter, addition C phase, stirs evenly to get shower cream.
Comparison study example 1
The preparation of hair conditioner
Each raw material, dosage and preparation method are with Application Example 1, and wherein Chinese rose extract is the preparation gained of comparative example 1.
Comparison study example 2
The preparation of shampoo
Each raw material, dosage and preparation method are with Application Example 2, and wherein Chinese rose extract is the preparation gained of comparative example 1.
Comparison study example 3
The preparation of spray
Each raw material, dosage and preparation method are with Application Example 3, and wherein Chinese rose extract is the preparation gained of comparative example 1.
Comparison study example 4
The preparation of shower cream
Each raw material, dosage and preparation method are with Application Example 4, and wherein Chinese rose extract is the preparation gained of comparative example 1.
Effect example 1
The Bactericidal test of Chinese rose extract inhibition chlosma
Experimental strain: Malassezia furfur (Malassezia furfur, ATCC44344)
Culture medium:
Chlosma solid medium: glucose 40g/L, agar 14g/L, peptone 10g/L, distilled water dissolution are adjusted
PH is 6.8~7.0, and sterilize 20min under 115 DEG C and condition of high voltage；
Chlosma fluid nutrient medium: glucose 40g/L, distilled water dissolution, adjusting PH is 6.8~7.0, at 115 DEG C and
Sterilize 20min under condition of high voltage.
Experimental method: by tested bacterium on chlosma solid medium after secondary culture, it is ensured that the purifying and work of bacterium colony
Power, picking monoclonal to 50mL chlosma fluid nutrient medium, 30 DEG C of cultures for 24 hours, are adjusted with than turbid instrument (Biomerieux SA)
It is whole its to the opacity tube concentration of Maxwell 4.Bacterium solution is diluted with fluid nutrient medium, makes its final bacteria concentration 106Then CFU/mL inhales
100uL is taken to be coated on solid medium.
Suppression of the Chinese rose extract in 1mg/mL obtained by Examples 1 to 3 and comparative example 1~3 is measured with Odontothrips loti
Bacterium circle size, to judge the bacteriostatic activity of Chinese rose extract.
Experimental result: Chinese rose extract is shown in Table 4 to the inhibiting effect of chlosma.
Table 4
As known from Table 4, the inhibition zone that the Chinese rose extract of Examples 1 to 3 forms chlosma is not less than 19mm, and
The Chinese rose extract of comparative example 1~3 is without inhibition zone.As it can be seen that Chinese rose extract of the invention have to chlosma it is good antibacterial
Effect can be formed using the Chinese rose extract of the invention of extremely low content to the biggish inhibition zone average value of chlosma.
Effect example 2
Chinese rose extract inhibits minimal inhibitory concentration (MIC value) measurement of chlosma
Experimental strain: Malassezia furfur (Malassezia furfur, ATCC44344)
Culture medium: chlosma fluid nutrient medium: glucose 40g/L, distilled water dissolution, adjusting ph is 6.8~7.0,
Sterilize 20min under 115 DEG C of condition of high voltage.
Experimental method: by tested bacterium on chlosma solid medium after secondary culture, with ensure bacterium colony purifying and
Vigor, picking monoclonal to 50mL chlosma fluid nutrient medium, 30 DEG C are cultivated for 24 hours, with than turbid instrument (Biomerieux SA)
It is adjusted to the opacity tube concentration of Maxwell 4.
Directly Chinese rose obtained by Examples 1 to 3 and comparative example 1~3 is extracted respectively with chlosma fluid nutrient medium
Object is diluted to experimental concentration, respectively 400,200,100,50,25,12.5 μ g/mL.100 μ of bacterium solution is added in 96 orifice-plate microporosities
100 μ L of L and medical fluid, while the negative control that bacterium is not added and the normal growth that medical fluid is not added are set to contrasting, every kind of medicine does 3 and puts down
Row, is averaged.It sets 30 DEG C of wet box to be incubated for, observes result after 48h.Since chlosma is thermophilic lipid, bacterium colony floats on liquid
Surface, yellowish, thin layer swims in transparent liquid-based surface, and it is very clear to visually observe, and reads data using direct method.
As a result the premise judged is that growth control is good, and blank control asepsis growth is clear, other holes with drug concentration gradient increase and
The growth of bacterium is suppressed.
Experimental result: bacterium growing state is shown in Table 5 under different Chinese rose extract concentrations.
Table 5
"-" represents asepsis growth；"+" representative has bacterium growth.
As shown in Table 5, the Chinese rose extract of Examples 1 to 3 is no more than 50 μ g/mL to the MIC value of chlosma, and compares
The Chinese rose extract of example 1~3 is >=400 μ g/mL to the MIC value of chlosma.As it can be seen that Chinese rose extract of the invention is to horse traction
Color bacterium has good fungistatic effect.
Effect example 3
Hair conditioner, shampoo, spray and the shower cream of the preparation of above-mentioned Application Example 1~4 are herein chatted having
It is carried out clinical use 15~20 days in the crowd for the skin symptom as caused by chlosma stated, all has good inhibit merely
Pityriasis type and seborrheica pityriasis type dandruff and psoriasis effect；Spray also has treatment atopic dermatitis, seborrhea
Effect.
Clinical research case:
Hair conditioner, shampoo and the spray of the preparation of above-mentioned Application Example 1~3 are applied to clinical research.
Tested crowd: scalp has more 30 people of dandruff person, 15 males, 15 women.
Experimental method: the hair conditioner of Examples 1 and 2 and shampoo being tested applied to anti-dandruff, wash primary head within every two days,
It is gently rubbed with shampoo hair 3 minutes, is massaged 5 minutes after cleaning with hair conditioner, then use the spray scalp 10 of embodiment 4
It is secondary.Subject's application form is given after 20 days carries out data statistics.
Experimental result: data statistics is carried out according to subject's application form, the results are shown in Table 6 after statistics.
Table 6
| Evaluation criterion | Number | Percentage |
| It cures and (is generated using after 20 days without dandruff) | 6 | 20% |
| Effectively (significantly reduced using dandruff after 20 days) | 15 | 50% |
| Generally (there is a small amount of reduction using dandruff after 20 days) | 6 | 20% |
| Nothing is substantially change and (is substantially change using nothing after 20 days) | 3 | 10% |
| It is very poor (more and more using dandruff after 20 days) | 0 | 0% |
It is carried out clinical use 20 days in being applied to the excessive crowd of dandruff, effective percentage is more than 70%, it is seen that system of the present invention
Standby shampoo, hair conditioner and spray has effects that good inhibition dandruff generates.
Claims (10)
1. a kind of preparation method of Chinese rose extract comprising following steps:
(1) it extracts China rose and obtains extracting solution, wherein solvent is 50~90% ethanol waters, the mass ratio of China rose and solvent
For 1:(6~15), Extracting temperature is 50~70 DEG C；
(2) extracting solution is filtered, filtrate is obtained；
(3) it is eluted after macroporous resin adsorption filtrate, obtains eluent；
(4) after concentrate eluant dry to get.
2. the preparation method of Chinese rose extract as described in claim 1, which is characterized in that in step (1), the solvent is 60
~80% ethanol water, preferably 70% ethanol water；
And/or the mass ratio of the China rose and solvent is 1:(8~12), preferably 1:10.
3. the preparation method of Chinese rose extract as described in claim 1, which is characterized in that in step (1), the Extracting temperature
It is 55~65 DEG C, preferably 60 DEG C；
And/or the time of the extraction is 1~3h, preferably 2h；
And/or it is described be extracted as decoct or recycle diafiltration, preferably circulation diafiltration.
4. the preparation method of Chinese rose extract as described in claim 1, which is characterized in that in step (2), the filtering is used
80~200 mesh screens, preferably 100 mesh；
It and/or further include that will be filtered again after the filtrate flocculation sedimentation in step (2)；
The preferable operating method of the flocculation sedimentation are as follows: flocculant is added in Xiang Suoshu filtrate, is stood after stirring；Wherein,
The flocculant is preferably chitosan, polyacrylamide or alum；
The additive amount of the flocculant is preferably 0.01~10%, more preferably for 0.01~0.05% or 0.05~
0.1% perhaps 0.1~0.5% perhaps 0.5~1% perhaps 1~5% or 5~10%, the percentage is the flocculation
Agent accounts for the mass percent of the filtrate；
The temperature of the stirring is preferably 30~50 DEG C, is more preferably 45 DEG C；
The time of the stirring is preferably 5~30min, is more preferably 10min；
The time of the standing is preferably 10~60min, is more preferably 30min.
5. the preparation method of Chinese rose extract as described in claim 1, which is characterized in that in step (3), the macroreticular resin
For non-polar macroporous resin or low pole macroreticular resin, preferably HP20 macroreticular resin, D101 macroreticular resin or AB-8 macropore
Resin；
And/or the eluant, eluent of the elution be 50~90% ethanol waters, preferably 60~80% ethanol waters, more
It goodly is 70% ethanol water.
6. the preparation method of Chinese rose extract as described in claim 1, which is characterized in that in step (4), the concentration is to subtract
Pressure concentration or normal pressure concentration；
And/or the drying is spray drying, vacuum drying or freeze-drying, is preferably dried in vacuo.
7. Chinese rose extract made from a kind of preparation method such as Chinese rose extract according to any one of claims 1 to 6.
8. a kind of product, it includes Chinese rose extracts as claimed in claim 7.
9. product as claimed in claim 8, which is characterized in that the mass fraction that the Chinese rose extract accounts for the product is
0.0001-50%, preferably 0.01-5% or 10~25% or 0.2~0.5%；
And/or the product is external preparation or chemical product；The preferable dosage form of external preparation is spray, ointment or cream
Cream；The chemical product is preferably shampoo, hair conditioner, cream, hand cleanser, shower cream or lotion.
10. a kind of drug of skin disease caused by Chinese rose extract as claimed in claim 7 treats chlosma in preparation
In application；
The chlosma causes skin disease to be preferably dandruff caused by chlosma excessive, atopic dermatitis, seborrheica
Dermatitis and psoriasis, the dandruff excessively include pityriasis simplex type and seborrheica pityriasis type；
The chlosma be preferably Malassezia furfur, M.pachy dermats, sympodium chlosma, Malassezia cilobosa,
One of obtuse chlosma, restricted chlosma and Si Luofei chlosma are a variety of.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1511583A (en) * | 2002-12-28 | 2004-07-14 | 石家庄制药集团制药技术开发有限公司 | Chinese rose extract and its preparing method and use |
| CN102949306A (en) * | 2012-06-29 | 2013-03-06 | 上海莱博生物科技有限公司 | Extractive of flowers as well as preparation method and application thereof |
| CN107375093A (en) * | 2017-08-16 | 2017-11-24 | 佛山市三水区嘉华化学研究院（普通合伙） | One kind resists the coarse anti-disposable spraying of bifurcated |
-
2019
- 2019-07-16 CN CN201910640014.2A patent/CN110201044A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1511583A (en) * | 2002-12-28 | 2004-07-14 | 石家庄制药集团制药技术开发有限公司 | Chinese rose extract and its preparing method and use |
| CN102949306A (en) * | 2012-06-29 | 2013-03-06 | 上海莱博生物科技有限公司 | Extractive of flowers as well as preparation method and application thereof |
| CN107375093A (en) * | 2017-08-16 | 2017-11-24 | 佛山市三水区嘉华化学研究院（普通合伙） | One kind resists the coarse anti-disposable spraying of bifurcated |
Non-Patent Citations (2)
| Title |
|---|
| ANILA GHANI ETAL: "Aqueous Extracts Of Common Plants Against Different Skin Problems Malassezia Furfur, Fusarium Proliferatum, Dryness, Acne, Rashes, Skin Burning", 《INTERNATIONAL JOURNAL OF SCIENTIFIC AND RESEARCH PUBLICATIONS》 * |
| 彼得.哈克尼斯: "《蔷薇秘事》", 30 September 2018, 商务书馆出版 * |
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