CN110199029B - 一种酶法制备瑞鲍迪甙c的方法 - Google Patents

一种酶法制备瑞鲍迪甙c的方法 Download PDF

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Publication number
CN110199029B
CN110199029B CN201680089981.9A CN201680089981A CN110199029B CN 110199029 B CN110199029 B CN 110199029B CN 201680089981 A CN201680089981 A CN 201680089981A CN 110199029 B CN110199029 B CN 110199029B
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Prior art keywords
udp
ugt
glycosyltransferase
rebaudioside
reaction
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Chinese (zh)
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CN110199029A (zh
Inventor
陶军华
李国庆
王文霞
郑雷雷
朱春磊
梁晓亮
陈车翘
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Pepsico Inc
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Pepsico Inc
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201680089981.9A 2016-10-21 2016-10-21 一种酶法制备瑞鲍迪甙c的方法 Active CN110199029B (zh)

Priority Applications (1)

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CN202311021375.1A CN117051063A (zh) 2016-10-21 2016-10-21 一种酶法制备瑞鲍迪甙c的方法

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PCT/CN2016/102910 WO2018072203A1 (zh) 2016-10-21 2016-10-21 一种酶法制备瑞鲍迪甙c的方法

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CN110199029A CN110199029A (zh) 2019-09-03
CN110199029B true CN110199029B (zh) 2023-09-05

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CN202311021375.1A Pending CN117051063A (zh) 2016-10-21 2016-10-21 一种酶法制备瑞鲍迪甙c的方法

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US (2) US11312985B2 (https=)
EP (1) EP3530746A4 (https=)
JP (1) JP2019532650A (https=)
CN (2) CN110199029B (https=)
AU (1) AU2016427120B2 (https=)
MX (1) MX2019004629A (https=)
RU (1) RU2736155C1 (https=)
WO (1) WO2018072203A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112019008048A2 (pt) 2016-10-21 2019-07-02 Pepsico Inc método para preparar rebaudiosídeo n com o uso de método enzimático
CN116574775A (zh) 2016-10-21 2023-08-11 百事可乐公司 一种酶法制备瑞鲍迪甙j的方法

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CN103179850A (zh) * 2010-06-02 2013-06-26 伊沃瓦营养学股份有限公司 甜菊糖苷的重组生产
CN103397064A (zh) * 2013-08-14 2013-11-20 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
CN103732753A (zh) * 2011-08-08 2014-04-16 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
CN103757074A (zh) * 2014-01-16 2014-04-30 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
AU2015261617A1 (en) * 2010-06-02 2015-12-17 Lallemand Bio-Ingredients Usa Llc Recombinant production of steviol glycosides
CN105200098A (zh) * 2015-06-30 2015-12-30 苏州汉酶生物技术有限公司 一种利用酿酒酵母酶法制备瑞鲍迪甙m的方法

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ES2381892T3 (es) 2007-01-22 2012-06-01 Cargill, Incorporated Procedimiento para producir composiciones de rebaudiósido A purificado que utiliza la cristalización disolvente/antidisolvente
CN101225424B (zh) 2007-09-13 2013-05-29 天津药物研究院 环黄芪醇的单葡萄糖苷、其制备方法、药物组合物和应用
ES2592377T3 (es) 2008-10-03 2016-11-29 Morita Kagaku Kogyo Co., Ltd. Nuevos glucósidos de esteviol
CA3015571C (en) 2009-10-15 2021-07-06 Purecircle Sdn Bhd High-purity rebaudioside d and applications
US8501261B2 (en) 2010-05-21 2013-08-06 Purecircle Sdn Bhd High-purity Rebaudioside C and process for purification of the same
US8962698B2 (en) 2011-01-28 2015-02-24 Tate & Lyle Ingredients Americas Llc Rebaudioside-mogroside V blends
CN103031283B (zh) 2011-10-08 2015-07-08 成都华高瑞甜科技有限公司 甜叶菊酶vi及莱鲍迪苷a转化为莱鲍迪苷d的方法
EP4124245A1 (en) 2011-12-19 2023-02-01 The Coca-Cola Company Beverage comprising rebaudioside x
MX355028B (es) 2012-01-23 2018-03-28 Dsm Ip Assets Bv Produccion de diterpeno.
MX395285B (es) 2012-05-22 2025-03-25 Purecircle Sdn Bhd Glucosidos de esteviol de alta pureza.
US9752174B2 (en) 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
EP2928321A1 (en) 2012-12-05 2015-10-14 Evolva SA Steviol glycoside compositions sensory properties
CN103088041B (zh) 2013-01-29 2015-01-07 江南大学 一种可用于高效生产角质酶的角质酶基因及其应用
MY199452A (en) 2013-02-06 2023-10-30 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
SG2013092820A (en) 2013-12-16 2015-07-30 Ngee Ann Polytechnic Xylose isomerase genes for xylose-fermenting yeast construction
CN106471128A (zh) 2014-01-28 2017-03-01 百事可乐公司 一种酶法制备瑞鲍迪甙m的方法
EP3683315A1 (en) 2014-08-19 2020-07-22 Purecircle SDN BHD Method for preparing rebaudioside m
US10612065B2 (en) 2015-05-29 2020-04-07 Cargill, Incorporated Heat treatment to produce glycosides
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BR112019008048A2 (pt) 2016-10-21 2019-07-02 Pepsico Inc método para preparar rebaudiosídeo n com o uso de método enzimático

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CN103179850A (zh) * 2010-06-02 2013-06-26 伊沃瓦营养学股份有限公司 甜菊糖苷的重组生产
AU2015261617A1 (en) * 2010-06-02 2015-12-17 Lallemand Bio-Ingredients Usa Llc Recombinant production of steviol glycosides
CN103732753A (zh) * 2011-08-08 2014-04-16 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
CN103397064A (zh) * 2013-08-14 2013-11-20 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
CN103757074A (zh) * 2014-01-16 2014-04-30 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
CN105200098A (zh) * 2015-06-30 2015-12-30 苏州汉酶生物技术有限公司 一种利用酿酒酵母酶法制备瑞鲍迪甙m的方法

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Also Published As

Publication number Publication date
CN117051063A (zh) 2023-11-14
CN110199029A (zh) 2019-09-03
US11976312B2 (en) 2024-05-07
WO2018072203A1 (zh) 2018-04-26
AU2016427120B2 (en) 2022-08-18
MX2019004629A (es) 2019-06-06
EP3530746A1 (en) 2019-08-28
EP3530746A4 (en) 2020-06-24
AU2016427120A8 (en) 2019-06-06
RU2736155C1 (ru) 2020-11-12
AU2016427120A1 (en) 2019-05-30
CA3041150A1 (en) 2018-04-26
US20190367960A1 (en) 2019-12-05
US20220220525A1 (en) 2022-07-14
US11312985B2 (en) 2022-04-26
JP2019532650A (ja) 2019-11-14

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