CN110151581B - Whitening composition containing glabridin and nanoemulsion preparation thereof - Google Patents
Whitening composition containing glabridin and nanoemulsion preparation thereof Download PDFInfo
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- A61K8/04—Dispersions; Emulsions
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
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- A61K8/67—Vitamins
- A61K8/676—Ascorbic acid, i.e. vitamin C
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- A61K8/678—Tocopherol, i.e. vitamin E
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Abstract
The invention discloses a whitening composition containing glabridin, a nanoemulsion preparation thereof and a preparation method thereof, wherein the whitening composition containing glabridin is prepared from glabridin, ferulic acid, vitamin E, panthenol, sodium hyaluronate, isopropyl myristate, disodium ethylene diamine tetraacetate, 2, 6-di-tert-butyl-4-methylphenol, ascorbic acid, lecithin, tween 80, ethylene glycol, phenoxyethanol, avocado oil, calendula oil and water. The whitening composition successfully solves the technical problem that the glabridin is safely and effectively applied to a cosmetic preparation as a whitening active ingredient.
Description
Technical Field
The invention relates to the technical field of cosmetics, and particularly relates to a whitening composition containing glabridin and a preparation method thereof.
Background
Skin pigmentation is a phenomenon which is caused by the combined influence of a series of external negative factors such as wind, rain, air pollution, sunlight exposure, weather dryness and the like and internal negative factors such as continuous aging, water loss and the like of the skin along with the aging, and the skin color looks dull and dull. With the continuous development of modern economy and the continuous improvement of the living standard of people, the majority of beauty lovers have higher and higher pursuits for having beautiful and white skin. Therefore, the art has long sought materials for cosmetic preparations that are safe and effective in improving skin pigmentation, restoring and maintaining the skin healthy, fair and shiny.
Glabridin (English name: Glabridin, CAS number: 59870-58-7), also known as licoflavone and Glabridin, is a natural component extracted from Glycyrrhiza glabra (Glycyrrhiza glabra L) and has the following structural formula:
glabridin, derived from Indian herbal medicine, was first used as a natural anti-inflammatory, anti-allergic ingredient. Later, the glabridin is scientifically found to be effective in inhibiting the formation of melanin in the skin and is the strongest and safest inhibitor of tyrosinase recognized so far (IC50 value, glabridin is 0.0045, Ju acid is 9.14, and vitamin C is 12.2). Meanwhile, the glabridin not only has the characteristics of safety and no irritation, but also has the effects of diminishing inflammation, resisting allergy, reducing pores and improving skin quality, so the glabridin is known as the whitening golden color and is one of the main components of high-end whitening products in the world at present.
However, glabridin itself has significant drawbacks, which greatly limits its use as a whitening active ingredient in cosmetics. First, glabridin has a relatively high molecular weight and is difficult to naturally permeate the skin to function. Although it is widely used in medicine, it is not effective as a skin care product. Secondly, glabridin is mainly derived from plant extracts. The production cost of glabridin is high due to the difficulty in planting the glycyrrhiza glabra. How to overcome the defects of glabridin and to enable the glabridin to be used as a whitening active ingredient to be safely and effectively applied to a cosmetic preparation becomes a problem to be solved by the technical personnel in the field.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects of the prior art and provide a whitening composition containing glabridin and a preparation method thereof, wherein the whitening composition containing glabridin is prepared from glabridin, ferulic acid, vitamin E, panthenol, sodium hyaluronate, isopropyl myristate, disodium ethylenediamine tetraacetic acid, 2, 6-di-tert-butyl-4-methylphenol, ascorbic acid, lecithin, tween 80, ethylene glycol, phenoxyethanol, avocado oil, calendula oil and water. The whitening composition successfully solves the technical problem that the glabridin is safely and effectively applied to a cosmetic preparation as a whitening active ingredient.
Therefore, the invention provides a whitening composition which is characterized by being a nanoemulsion prepared from the following raw materials in parts by weight:
glabridin 0.5-5
Ferulic acid 1-3
Vitamin E1-3
Panthenol 0.5-5
0.1-1 parts of sodium hyaluronate
Isopropyl myristate 0.1-1
Disodium ethylene diamine tetraacetate 0.5-1.5
2, 6-di-tert-butyl-4-methylphenol 0.1-1
Ascorbic acid 0.1-1
Lecithin 15-20
Tween 8010-15
Ethylene glycol 5-10
0.1-0.8 parts of phenoxyethanol
Avocado oil 25-30
Calendula oil 10-15
Water 120-
The preparation method comprises the following steps: (1) mixing panthenol, sodium hyaluronate, disodium edetate, ascorbic acid, tween 80, ethylene glycol, phenoxyethanol and water at 25-35 deg.C, and stirring to obtain water phase; (2) mixing glabridin, ferulic acid, vitamin E, isopropyl myristate, 2, 6-di-tert-butyl-4-methylphenol, lecithin, avocado oil and calendula oil at 25-35 deg.C, and stirring to obtain oil phase; (3) adding the oil phase prepared in the step (2) into the water phase prepared in the step (1) under the condition of vigorous stirring at 25-35 ℃, putting the mixture into an ultrasonic generator, and mixing the mixture for 15-25min at room temperature under the high-frequency oscillation of 40-50Hz, thereby obtaining the whitening composition in the form of the nanoemulsion.
In one embodiment, the whitening composition is a nanoemulsion prepared from the following raw materials in parts by weight:
glabridin 0.5
Ferulic acid 1
Vitamin E1
Panthenol 0.5
Hyaluronic acid sodium salt 0.1
Isopropyl myristate 0.1
Disodium ethylenediaminetetraacetate 0.5
2, 6-di-tert-butyl-4-methylphenol 0.1
Ascorbic acid 0.1
Lecithin 15
Tween 8010
Ethylene glycol 5
0.1 part of phenoxyethanol
Avocado oil 25
Calendula oil 10
And (3) water 120.
In one embodiment, the whitening composition is a nanoemulsion prepared from the following raw materials in parts by weight:
glabridin 2.5
Ferulic acid 2
Vitamin E2
Panthenol 2.5
0.5 parts of sodium hyaluronate
Isopropyl myristate 0.5
Ethylenediaminetetraacetic acid disodium 1
2, 6-di-tert-butyl-4-methylphenol 0.5
Ascorbic acid 0.5
Lecithin 17.5
Tween 8012.5
Ethylene glycol 7.5
0.45 parts of phenoxyethanol
Avocado oil 27.5
Calendula oil 12.5
And (4) water 125.
In one embodiment, the whitening composition is a nanoemulsion prepared from the following raw materials in parts by weight:
glabridin 5
Ferulic acid 3
Vitamin E3
Panthenol 5
Sodium hyaluronate 1
Isopropyl myristate 1
Disodium ethylenediaminetetraacetate 1.5
2, 6-di-tert-butyl-4-methylphenol 1
Ascorbic acid 1
Lecithin 20
Tween 8015
Ethylene glycol 10
0.8 parts of phenoxyethanol
Avocado oil 30
Calendula oil 15
And (c) water 130.
In one embodiment, the present invention also provides a method for preparing the whitening composition of the present invention, the method comprising: (1) mixing panthenol, sodium hyaluronate, disodium edetate, ascorbic acid, tween 80, ethylene glycol, phenoxyethanol and water at 25-35 deg.C, and stirring to obtain water phase; (2) mixing glabridin, ferulic acid, vitamin E, isopropyl myristate, 2, 6-di-tert-butyl-4-methylphenol, lecithin, avocado oil and calendula oil at 25-35 deg.C, and stirring to obtain oil phase; (3) adding the oil phase prepared in the step (2) into the water phase prepared in the step (1) under the condition of vigorous stirring at 25-35 ℃, putting the mixture into an ultrasonic generator, and mixing the mixture for 15-25min at room temperature under the high-frequency oscillation of 40-50Hz, thereby obtaining the whitening composition in the form of the nanoemulsion.
The present invention also relates to a method for whitening skin, characterized in that the method comprises applying the whitening composition according to the present invention to a subject in need thereof.
The invention has the following beneficial effects:
1. the novel nanoemulsion formulation of the present invention changes the conventional permeation pattern and has improved biocompatibility, so that the active ingredient can stay in the skin tissue more and for a longer time, rather than being lost with the blood circulation.
2. The novel nanoemulsion preparation improves the effectiveness and the utilization rate of the glabridin, and can reduce the use amount of the glabridin, thereby reducing the manufacturing cost.
3. The novel nanoemulsion preparation adopts a ternary antioxidant stabilization system formed by compounding disodium ethylene diamine tetraacetate, 2, 6-di-tert-butyl-4-methylphenol (BHT) and ascorbic acid, successfully solves the problems that glabridin is not resistant to light and is easily oxidized and yellowed by air, and thus the storage life of the product is prolonged to three years.
4. The whitening composition prepared by the method has an excellent whitening effect, can obviously inhibit skin pigmentation, and can be used for daily care of sunlight-sensitive skin.
In order to further understand the spirit and gist of the present invention, those skilled in the art will be described below with reference to specific examples and experimental examples to describe preferred embodiments of the present invention and effects thereof. It is to be understood, however, that the description is intended only to further illustrate features and advantages of the present invention, and not to limit in any way the claims of the present invention.
Detailed Description
Example 1
Whitening composition 1
Weighing 0.5g of glabridin, 1g of ferulic acid, 1g of vitamin E, 0.5g of panthenol, 0.1g of sodium hyaluronate, 0.1g of isopropyl myristate, 0.5g of disodium ethylenediamine tetraacetic acid, 0.1g of 2, 6-di-tert-butyl-4-methylphenol, 0.1g of ascorbic acid, 15g of lecithin, 8010 g of tween, 5g of ethylene glycol, 0.1g of phenoxyethanol, 25g of avocado oil, 10g of calendula oil and 120g of water, and preparing the whitening composition of the embodiment according to the following method: (1) mixing panthenol, sodium hyaluronate, disodium edetate, ascorbic acid, tween 80, ethylene glycol, phenoxyethanol and water at 30 deg.C, and stirring to obtain water phase; (2) fully mixing glabridin, ferulic acid, vitamin E, isopropyl myristate, 2, 6-di-tert-butyl-4-methylphenol, lecithin, avocado oil and calendula oil at 30 ℃, and stirring uniformly to form an oil phase; (3) adding the oil phase prepared in the step (2) into the water phase prepared in the step (1) under the condition of vigorous stirring at 30 ℃, putting the mixture into an ultrasonic generator, and mixing the mixture at room temperature under the high-frequency oscillation of 45Hz for 20min to obtain the whitening composition in the form of the nanoemulsion.
Example 2
Whitening composition 2
Weighing 2.5g of glabridin, 2g of ferulic acid, 2g of vitamin E, 2.5g of panthenol, 0.5g of sodium hyaluronate, 0.5g of isopropyl myristate, 1g of disodium ethylenediamine tetraacetic acid, 0.5g of 2, 6-di-tert-butyl-4-methylphenol, 0.5g of ascorbic acid, 17.5g of lecithin, 8012.5 g of Tween, 7.5g of ethylene glycol, 0.45g of phenoxyethanol, 27.5g of avocado oil, 12.5g of calendula oil and 125g of water, preparing the whitening composition of the embodiment according to the following method: (1) mixing panthenol, sodium hyaluronate, disodium edetate, ascorbic acid, tween 80, ethylene glycol, phenoxyethanol and water at 30 deg.C, and stirring to obtain water phase; (2) fully mixing glabridin, ferulic acid, vitamin E, isopropyl myristate, 2, 6-di-tert-butyl-4-methylphenol, lecithin, avocado oil and calendula oil at 30 ℃, and stirring uniformly to form an oil phase; (3) adding the oil phase prepared in the step (2) into the water phase prepared in the step (1) under the condition of vigorous stirring at 30 ℃, putting the mixture into an ultrasonic generator, and mixing the mixture at room temperature under the high-frequency oscillation of 45Hz for 20min to obtain the whitening composition in the form of the nanoemulsion.
Example 3
Whitening composition 3
Weighing 5g of glabridin, 3g of ferulic acid, 3g of vitamin E, 5g of panthenol, 1g of sodium hyaluronate, 1g of isopropyl myristate, 1.5g of disodium ethylenediamine tetraacetic acid, 1g of 2, 6-di-tert-butyl-4-methylphenol, 1g of ascorbic acid, 20g of lecithin, 8015 g of tween, 10g of ethylene glycol, 0.8g of phenoxyethanol, 30g of avocado oil, 15g of calendula oil and 130g of water, and preparing the whitening composition of the embodiment according to the following method: (1) mixing panthenol, sodium hyaluronate, disodium edetate, ascorbic acid, tween 80, ethylene glycol, phenoxyethanol and water at 30 deg.C, and stirring to obtain water phase; (2) fully mixing glabridin, ferulic acid, vitamin E, isopropyl myristate, 2, 6-di-tert-butyl-4-methylphenol, lecithin, avocado oil and calendula oil at 30 ℃, and stirring uniformly to form an oil phase; (3) adding the oil phase prepared in the step (2) into the water phase prepared in the step (1) under the condition of vigorous stirring at 30 ℃, putting the mixture into an ultrasonic generator, and mixing the mixture at room temperature under the high-frequency oscillation of 45Hz for 20min to obtain the whitening composition in the form of the nanoemulsion.
Comparative example 1
Weighing 2.5g of glabridin, 2.5g of panthenol, 0.5g of sodium hyaluronate, 0.5g of isopropyl myristate, 1g of ethylene diamine tetraacetic acid disodium, 0.5g of 2, 6-di-tert-butyl-4-methylphenol, 0.5g of ascorbic acid, 17.5g of lecithin, 8012.5 g of tween, 7.5g of glycol, 0.45g of phenoxyethanol, 27.5g of avocado oil, 12.5g of calendula oil and 125g of water, preparing the whitening composition of the comparative example according to the following method: (1) mixing panthenol, sodium hyaluronate, disodium edetate, ascorbic acid, tween 80, ethylene glycol, phenoxyethanol and water at 30 deg.C, and stirring to obtain water phase; (2) fully mixing glabridin, isopropyl myristate, 2, 6-di-tert-butyl-4-methylphenol, lecithin, avocado oil and calendula oil at 30 ℃, and stirring uniformly to form an oil phase; (3) adding the oil phase prepared in the step (2) into the water phase prepared in the step (1) under the condition of vigorous stirring at 30 ℃, putting the mixture into an ultrasonic generator, and mixing the mixture for 20min at room temperature under the high-frequency oscillation of 45Hz, thereby obtaining the whitening composition in the form of the nanoemulsion according to the comparative example.
Comparative example 2
Weighing 2.5g of glabridin, 2g of ferulic acid, 2.5g of panthenol, 0.5g of sodium hyaluronate, 0.5g of isopropyl myristate, 1g of disodium ethylenediamine tetraacetic acid, 0.5g of 2, 6-di-tert-butyl-4-methylphenol, 0.5g of ascorbic acid, 17.5g of lecithin, 8012.5 g of tween, 7.5g of ethylene glycol, 0.45g of phenoxyethanol, 27.5g of avocado oil, 12.5g of calendula oil and 125g of water, and preparing the whitening composition of the comparative example according to the following method: (1) mixing panthenol, sodium hyaluronate, disodium edetate, ascorbic acid, tween 80, ethylene glycol, phenoxyethanol and water at 30 deg.C, and stirring to obtain water phase; (2) fully mixing glabridin, ferulic acid, isopropyl myristate, 2, 6-di-tert-butyl-4-methylphenol, lecithin, avocado oil and calendula oil at 30 ℃, and stirring uniformly to form an oil phase; (3) adding the oil phase prepared in the step (2) into the water phase prepared in the step (1) under the condition of vigorous stirring at 30 ℃, placing the mixture into an ultrasonic generator, and mixing the mixture for 20min at room temperature by high-frequency oscillation at 45Hz, thereby obtaining the whitening composition in the form of the nano emulsion in the comparative example.
Comparative example 3
Weighing 2.5g of glabridin, 2g of vitamin E, 2.5g of panthenol, 0.5g of sodium hyaluronate, 0.5g of isopropyl myristate, 1g of disodium ethylenediamine tetraacetic acid, 0.5g of 2, 6-di-tert-butyl-4-methylphenol, 0.5g of ascorbic acid, 17.5g of lecithin, 8012.5 g of Tween, 7.5g of ethylene glycol, 0.45g of phenoxyethanol, 27.5g of avocado oil, 12.5g of calendula oil and 125g of water, and preparing the whitening composition of the comparative example according to the following method: (1) mixing panthenol, sodium hyaluronate, disodium edetate, ascorbic acid, tween 80, ethylene glycol, phenoxyethanol and water at 30 deg.C, and stirring to obtain water phase; (2) fully mixing glabridin, vitamin E, isopropyl myristate, 2, 6-di-tert-butyl-4-methylphenol, lecithin, avocado oil and calendula oil at 30 ℃, and stirring uniformly to form an oil phase; (3) adding the oil phase prepared in the step (2) into the water phase prepared in the step (1) under the condition of vigorous stirring at 30 ℃, putting the mixture into an ultrasonic generator, and mixing the mixture for 20min at room temperature under the high-frequency oscillation of 45Hz, thereby obtaining the whitening composition in the form of the nanoemulsion according to the comparative example.
Comparative example 4
Weighing 2.5g of glabridin, 2g of ferulic acid, 2g of vitamin E, 2.5g of panthenol, 0.5g of sodium hyaluronate, 0.5g of isopropyl myristate, 17.5g of lecithin, 8012.5 g of Tween, 7.5g of ethylene glycol, 0.45g of phenoxyethanol, 27.5g of avocado oil, 12.5g of calendula oil and 125g of water, and preparing the whitening composition of the comparative example according to the following method: (1) mixing panthenol, sodium hyaluronate, tween 80, ethylene glycol, phenoxyethanol and water at 30 deg.C, and stirring to obtain water phase; (2) fully mixing glabridin, ferulic acid, vitamin E, isopropyl myristate, lecithin, avocado oil and calendula oil at 30 ℃, and stirring uniformly to form an oil phase; (3) adding the oil phase prepared in the step (2) into the water phase prepared in the step (1) under the condition of vigorous stirring at 30 ℃, putting the mixture into an ultrasonic generator, and mixing the mixture for 20min at room temperature under the high-frequency oscillation of 45Hz, thereby obtaining the whitening composition in the form of the nanoemulsion according to the comparative example.
Comparative example 5
Weighing 2.5g of glabridin, 2g of ferulic acid, 2g of vitamin E, 2.5g of panthenol, 0.5g of sodium hyaluronate, 0.5g of isopropyl myristate, 1g of disodium ethylenediamine tetraacetic acid, 17.5g of lecithin, 8012.5 g of Tween, 7.5g of ethylene glycol, 0.45g of phenoxyethanol, 27.5g of avocado oil, 12.5g of calendula oil and 125g of water, and preparing the whitening composition of the comparative example according to the following method: (1) mixing panthenol, sodium hyaluronate, disodium edetate, tween 80, ethylene glycol, phenoxyethanol and water at 30 deg.C, and stirring to obtain water phase; (2) fully mixing glabridin, ferulic acid, vitamin E, isopropyl myristate, lecithin, avocado oil and calendula oil at 30 ℃, and stirring uniformly to form an oil phase; (3) adding the oil phase prepared in the step (2) into the water phase prepared in the step (1) under the condition of vigorous stirring at 30 ℃, putting the mixture into an ultrasonic generator, and mixing the mixture for 20min at room temperature under the high-frequency oscillation of 45Hz, thereby obtaining the whitening composition in the form of the nanoemulsion according to the comparative example.
Comparative example 6
Weighing 2.5g of glabridin, 2g of ferulic acid, 2g of vitamin E, 2.5g of panthenol, 0.5g of sodium hyaluronate, 0.5g of isopropyl myristate, 1g of disodium ethylenediamine tetraacetic acid, 0.5g of 2, 6-di-tert-butyl-4-methylphenol, 17.5g of lecithin, 8012.5 g of tween, 7.5g of ethylene glycol, 0.45g of phenoxyethanol, 27.5g of avocado oil, 12.5g of calendula oil and 125g of water, and preparing the whitening composition of the comparative example according to the following method: (1) mixing panthenol, sodium hyaluronate, disodium edetate, tween 80, ethylene glycol, phenoxyethanol and water at 30 deg.C, and stirring to obtain water phase; (2) fully mixing glabridin, ferulic acid, vitamin E, isopropyl myristate, 2, 6-di-tert-butyl-4-methylphenol, lecithin, avocado oil and calendula oil at 30 ℃, and stirring uniformly to form an oil phase; (3) adding the oil phase prepared in the step (2) into the water phase prepared in the step (1) under the condition of vigorous stirring at 30 ℃, putting the mixture into an ultrasonic generator, and mixing the mixture for 20min at room temperature under the high-frequency oscillation of 45Hz, thereby obtaining the whitening composition in the form of the nanoemulsion according to the comparative example.
Experimental example 1 whitening efficacy of the whitening composition of the present invention
The purpose of this experimental example was to investigate whether the whitening composition prepared according to the example of the present invention has whitening efficacy.
1. Investigation mode
The experiment was recruited to 72 subjects between the ages of 25-39 years, 38 males and 34 females. The subject was identified by a dermatologist as having a good skin condition.
2. Experimental methods
(1) Establishing a model: an opaque tape having 3 through holes of 2.0 cm in diameter was adhered to the back side of the forearm of each subject, and UV irradiation (irradiation dose 1.5 times the minimum erythema dose) was applied to the portion of skin to which the tape was adhered to induce skin melanin pigmentation.
(2) And (3) random grouping: the subjects were randomized into 6 groups of 12 people each. Groups 1, 2 and 3 are experimental groups to which whitening compositions 1, 2 and 3 prepared according to examples 1, 2 and 3 of the present invention were applied, respectively. Groups 4, 5 and 6 are experimental controls to which the products prepared according to comparative examples 1, 2 and 3 of the present invention were applied, respectively.
(3) The application method comprises the following steps: after cleaning the skin every morning and evening, the test article was dipped in cotton wool and applied to the UV-irradiated area for 3 months.
(4) And (4) evaluating the result: after 3 months, the skin color of the subjects was measured with a commercially available colorimeter, and the effect of each composition was judged by measuring the "L" value reflecting the lightness and darkness of the skin, which was measured by a professional, and correcting the measurement results by a standard color plate. The difference in skin color (Δ L) from before the start of the experiment to when the application of the test article was completed was calculated according to the following formula: Δ L is the luminance at the completion of the application of the test article L — the luminance before the start of the experiment. See table 1 for results.
TABLE 1 color difference of skin (Δ L) from the beginning of experiment to the completion of test article application for each experimental group
| Test article | Skin color difference (Delta L) |
| Example 1 | 2.56 |
| Example 2 | 2.87 |
| Example 3 | 3.12 |
| Comparative example 1 | 1.59 |
| Comparative example 2 | 1.85 |
| Comparative example 3 | 1.73 |
In general, when the whitening effect is evaluated by a value of a color difference of skin (Δ L), if the Δ L value exceeds 1.5, it indicates a certain whitening effect, and when the Δ L value is greater than 2, it indicates a significant alleviation of skin pigmentation. As is apparent from table 1 above, the whitening composition prepared in the form of a nanoemulsion according to the present invention has an obvious skin whitening effect in terms of whitening effect, and the effect is significantly superior to that of glabridin prepared alone (corresponding to comparative example 1) or a combination of glabridin and any one of ferulic acid and vitamin E (corresponding to comparative example 2 and comparative example 3, respectively), which indicates that when glabridin is compounded with ferulic acid and vitamin E in a specific ratio, there is an obvious synergistic effect to further increase the whitening effect of glabridin, thereby suggesting that the same whitening effect can be achieved with a smaller amount of glabridin, and thus the amount of glabridin used can be reduced, thereby reducing the manufacturing cost.
Experimental example 2 stability over time study of glabridin preparation
The stability of glabridin over time (1 month, 3 months, 1 year and 3 years) in example 2 and comparative examples 4, 5 and 6 was examined by visual inspection to determine the possible effect of the ternary antioxidant stabilization system consisting of disodium ethylenediaminetetraacetate, 2, 6-di-tert-butyl-4-methylphenol and ascorbic acid adopted in the present invention on the stability of glabridin.
50ml of each of the nanoemulsion formulations of example 2 and comparative examples 4, 5 and 6 were placed in a display room maintained at 25 ℃ under 1 atmosphere of pressure and subjected to daily light, and then the appearance change of the test article was observed at time points of 1 month, 3 months, 1 year and 3 years, respectively.
The stability test results are as follows:
TABLE 2 change in appearance over time of glabridin formulations
The above results indicate that glabridin (corresponding to comparative example 4) in the nanoemulsion formulation is unstable to light and is easily oxidized to yellow to be ineffective. When glabridin was formulated together with disodium ethylenediaminetetraacetate (corresponding to comparative example 5), the light stability of glabridin was enhanced. This light stability can be further enhanced by the addition of 2, 6-di-tert-butyl-4-methylphenol (corresponding to comparative example 6). The invention adopts a ternary antioxidant stabilization system which is compounded by disodium ethylene diamine tetraacetate, 2, 6-di-tert-butyl-4-methylphenol and ascorbic acid, successfully solves the problem that the glabridin is easy to oxidize and turn yellow, thereby obviously prolonging the shelf life of the product to three years. The above results are not described or suggested in the prior art, thereby providing substantial benefits not anticipated by the present invention.
The foregoing is only a preferred embodiment of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit and principle of the present invention, several improvements, modifications, equivalents and the like can be made, and these improvements, modifications, equivalents and the like also should be regarded as falling within the protection scope of the present invention.
Claims (1)
1. The whitening composition is characterized by being a nanoemulsion prepared from the following raw materials in parts by weight:
glabridin 0.5
Ferulic acid 1
Vitamin E1
Panthenol 0.5
Hyaluronic acid sodium salt 0.1
Isopropyl myristate 0.1
Disodium ethylenediaminetetraacetate 0.5
2, 6-di-tert-butyl-4-methylphenol 0.1
Ascorbic acid 0.1
Lecithin 15
Tween 8010
Ethylene glycol 5
0.1 part of phenoxyethanol
Avocado oil 25
Calendula oil 10
The amount of water (120) is,
the preparation method comprises the following steps: (1) mixing panthenol, sodium hyaluronate, disodium edetate, ascorbic acid, tween 80, ethylene glycol, phenoxyethanol and water at 25-35 deg.C, and stirring to obtain water phase; (2) mixing glabridin, ferulic acid, vitamin E, isopropyl myristate, 2, 6-di-tert-butyl-4-methylphenol, lecithin, avocado oil and calendula oil at 25-35 deg.C, and stirring to obtain oil phase; (3) adding the oil phase prepared in the step (2) into the water phase prepared in the step (1) under the condition of vigorous stirring at 25-35 ℃, putting the mixture into an ultrasonic generator, and mixing the mixture for 15-25min at room temperature under the high-frequency oscillation of 40-50Hz, thereby obtaining the whitening composition in the form of the nanoemulsion.
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| CN106691888A (en) * | 2015-11-12 | 2017-05-24 | 武汉百思凯瑞纳米科技有限公司 | High-skin-retention glabridin nano composition as well as preparation method and application thereof |
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| CN106691888A (en) * | 2015-11-12 | 2017-05-24 | 武汉百思凯瑞纳米科技有限公司 | High-skin-retention glabridin nano composition as well as preparation method and application thereof |
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