CN110124094A - A kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing be prepared in situ and application method - Google Patents
A kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing be prepared in situ and application method Download PDFInfo
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- CN110124094A CN110124094A CN201910319815.9A CN201910319815A CN110124094A CN 110124094 A CN110124094 A CN 110124094A CN 201910319815 A CN201910319815 A CN 201910319815A CN 110124094 A CN110124094 A CN 110124094A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0014—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
- A61L26/0047—Specific proteins or polypeptides not covered by groups A61L26/0033 - A61L26/0042
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0057—Ingredients of undetermined constitution or reaction products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/413—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties containing granules other than absorbent substances
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4382—Stretched reticular film fibres; Composite fibres; Mixed fibres; Ultrafine fibres; Fibres for artificial leather
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- Animal Behavior & Ethology (AREA)
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Abstract
The invention discloses a kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing be prepared in situ and application method, including with ethyl alcohol, polymeric is completely dissolved by deionized water equal solvent, Polymer Solution is made, Chinese herbal medicine extract is dissolved or is distributed in Polymer Solution and obtains spinning precursor liquid after stirring to substantially uniformity, prepared spinning precursor liquid is added in syringe, and syringe is put into portable electrostatic device for spinning, open equipment, spinning parameter is adjusted to there is fiber uniformly to spray, it controls nozzle mouth and receives the spacing distance of wound, it may be implemented in wound and nanofiber antiseptic dressing be prepared in situ.Anti-bacterial fibre film dressing perfection can be bonded wound using the effect of electrostatic force by the preparation method, and the nano fibrous membrane has good gas permeability.Meanwhile using Chinese herbal medicine extract as antimicrobial component, low in cost, good anti-bacterial effect has no toxic side effect, nonirritant.
Description
Technical field
The invention belongs to medical dressing fields, and in particular to a kind of system in situ of Chinese herbal medicine based micro-nano fibre antibacterial dressing
Standby and application method.
Background technique
Skin is the maximum organ of human body, is the first barrier of protecting human body, it makes in vivo, and various tissues and organ are exempted from
By physical, mechanicalness, chemically with the invasion of pathogenic microorganism, but its moment is exposed in external environment and will lead to difference
The damage of type, mainly includes acute (such as operation and traumatic wound, scratch and burn) and chronic (such as diabetes are routed
Ulcer and pressure sore).According to the estimation of the World Health Organization (WHO), there are about 41,000,000 wound cases, annual economy is negative in the annual whole world
Load is more than 670,000,000,000 dollars.As it can be seen that human skin wound to global medical health system bring it is sizable society and
Financial burden.
The treatment of skin wound depends primarily on the size of wound, depth and extent of the destruction, and dependent on wound dressing
Auxiliary.It includes: that can be suitble to shape of wound that ideal wound dressing, which needs to have various features, absorbs wound fluid without increasing
Bacterial multiplication causes excessive dehydration, controls the liquids and gases of outflow, protects wound and surrounding tissue, eliminates pain, promotes
Reparation phase re-epithelialization is easy to apply and remove and patient is made to feel comfortable.Domestic inventor has been developed at present
A variety of wound dressings include as Chinese invention patent (CN201310613174.0) discloses a kind of high penetration rate film dressing
Polyurethane film and coating, Chinese invention patent (CN201310655235.X) disclose a kind of silver ionized water gel dressing
Preparation method, Chinese invention patent (CN201510130794.8) disclose a kind of foam dressing containing compound silver-colored zinc anti-biotic material
And preparation method thereof equal foam dressings and hydrophilic fibers dressing etc., it is such as ventilative more or less to there are some problems in these dressing
Property, wellability, antibiotic property and the inconvenience of replacement etc., and lack the ability for reappearing Skin Cell epimatrix (ECM) structure.
In recent years, due to high specific surface area, high porosity and morphology controllable etc. are excellent for electrospinning fibre and its tunica fibrosa
Point causes many concerns in terms of wound healing, electrospun fiber membrane on the one hand can physical isolation virus and bacterium, and can
Air permeable humidity retaining builds a good healing environment to wound；On the other hand, the aperture of the diameter of electrospinning fibre and tunica fibrosa
With the comparable dimensions of extracellular matrix, cell can be promoted to grow, accelerating wound healing speed reduces scar and generates, therefore
There is unique advantage in terms of wound dressing.In terms of electrospinning fibre is used as antiseptic dressing, Chinese invention patent
(CN201310574818.X) a kind of preparation method of chitosan-based electrostatic spinning combined wound dressing is disclosed, Chinese invention is special
Sharp (CN201610479265.3) discloses a kind of method for preparing imitative extracellular matrix nanofiber dressing using electrostatic spinning,
Chinese invention patent (CN201710076435.8) discloses a kind of preparation method etc. of antibacterial nano fiber wound dressing.However
The preparation and application of these electrospinning fibre film dressings are all divided into two steps, i.e. the first step prepares electrospun fiber membrane, and second step will be fine
Dimension film dressing is applied at skin trauma.This traditional application method proposes very high requirement to nursing staff, i.e., cannot
Secondary injury is caused to wound, and fiber film dressing is difficult to be bonded well with the surface of a wound.And the antibacterial of these anti-bacterial fibre dressing
Property is mostly from the inorganic antibacterials ingredient such as Nano silver grain, and for the toxicity of these nanoparticles, there are still disputes at present.
Summary of the invention
The technical problem to be solved by the present invention is overcome prior art defect, providing one kind, that antibacterial is prepared in situ is micro-nano
The method of fiber dressing, this dressing has good antibacterial, permeability, can promote wound healing, convenient to use,
It can be bonded the various types surface of a wound, complete preservation is provided to the surface of a wound, be particularly suitable for large area dispersivity wound care.
To solve the above-mentioned problems, being prepared in situ the present invention provides a kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing
With application method, comprising the following steps:
Step 1: polymeric being completely dissolved with dehydrated alcohol, Polymer Solution is made；The polymer is high
Molecule is polyethylene Topiramate Los oxazolidinone PVP, polyvinyl butyral PVB and zein.Polymer Solution concentration is 8-15wt%；
Step 2: height prepared by step 1 is added to after Chinese herbal medicine extract is dissolved into deionized water or dehydrated alcohol
Be the 1-5wt% of polymer content to the quality of Chinese herbal medicine in molecular solution, stirring to obtaining spinning precursor liquid after substantially uniformity,
The Chinese herbal medicine extract includes isatis root extract, Folium Isatidis extract, Honegsukle flower P.E, coptis extract, and radix scutellariae mentions
Take object, forsythia suspense extraction etc.；
Step 3: spinning precursor liquid prepared in step 2 being added in syringe, and syringe is put into portable
In electrostatic spinning apparatus, open equipment, adjust spinning parameter, control nozzle mouth and receive wound spacing distance, it can be achieved that
Nanofiber antiseptic dressing is prepared in situ in wound.
Further, the syringe is 5-10ml injector for medical purpose, and it is the flat of 0.3-1.5mm that syringe needle, which selects internal diameter,
Head metal needle；The portable electric spinning equipment is hand-held electric spinning equipment or the electrospinning in situ dress with hand-held spray head
It sets.
It further, include 8-20 kilovolts of spinning voltage in spinning parameter, 5-15 centimetres of spinning distance.
Further, electro spinning nano fiber antiseptic dressing in situ with a thickness of 80-1000 microns.
The beneficial effect of the preparation method is that it is micro- directly can to deposit antibacterial in wound using electrostatic spinning technique in situ
Anti-bacterial fibre film dressing perfection can be bonded wound using the effect of electrostatic force by nano fibrous membrane, and the nano fibrous membrane has
There is good gas permeability.Meanwhile using Chinese herbal medicine as antimicrobial component, low in cost, good anti-bacterial effect has no toxic side effect, nothing
Irritation.
Detailed description of the invention
Fig. 1: PVP/ Radix Isatidis nanofiber antiseptic dressing scanning electron microscope sem picture prepared by the present invention；
Fig. 2: PVB/ coptis nanofiber antiseptic dressing scanning electron microscope sem picture prepared by the present invention；
Fig. 3: zein prepared by the present invention/honeysuckle nanofiber antiseptic dressing scanning electron microscope sem picture.
Specific embodiment
In order to clarify the technical characteristics of the invention, being illustrated below by embodiment to this programme.Implementing
Be respectively adopted in example 1,2 and embodiment 3 a kind of portable hand-held electrostatic spinning apparatus (China Patent No.:
CN201210229010.3, CN201510214221.3) and a kind of electrostatic spinning apparatus in situ (China Patent No.:
The portable electrostatics device for spinning such as CN201410560189.X).
Embodiment 1:
Step 1: weighing 1.5 grams of polyvinylpyrrolidonepowder powders, (ten thousand) PVP, molecular weight 130, are all dissolved in 8.5 grams of nothings
In water-ethanol, magnetic agitation 8 hours to being completely dissolved at room temperature, obtains the uniform PVP spinning that Solute mass fraction is 15wt%
Precursor liquid；
Step 2: weighing 2.0 grams of isatis root extracts and be distributed in 8.0 grams of dehydrated alcohols, at room temperature magnetic agitation 8 hours
It is extremely evenly dispersed, obtain the uniform suspension that Solute mass fraction is 20wt%；
Step 3: suspension obtained in step 2 being added in spinning precursor liquid prepared by step 1, the amount of addition makes molten
The mass percentage of Radix Isatidis is 5% in liquid, is stirred 2 hours, it is uniformly mixed, then is made after standing 12 hours uniformly steady
Fixed spinning precursor liquid；
Step 4: spinning precursor liquid prepared in step 3 is added in 5ml syringe (1.0 millimeters of needle diameter),
And be installed on hand-held electrostatic spinning apparatus, device is grasped, is turned on the power switch, thumb gently presses injector push-rod, adjustment
Distance carries out spinning under conditions of being 6 centimetres from wound, directly deposits PVP/ Radix Isatidis micro nanometer fiber antibacterial in wound
Dressing, obtained PVP/ Radix Isatidis nano fibrous membrane is with a thickness of 100 microns, and fibre diameter is about 500 nanometers, and fiber morphology is such as
Shown in Fig. 1.
It is 7.5 degree with the contact angle of the PVP/ Radix Isatidis tunica fibrosa prepared in simulated body fluid SBF testing procedure 4, shows original
The PVP/ Radix Isatidis of position electrospinning preparation has good wellability, can quickly absorb the diffusate of wound, keep wound moist.
The gas permeability of PVP/ Radix Isatidis tunica fibrosa is 124.7 ± 11.43 squares of metre per second (m/s)s in testing procedure 4, than traditional adhesive bandage
Gas permeability is 8 times high.The PVP/ Radix Isatidis nanofiber prepared in step 4 is placed on the aseptic filter paper that diameter is 5 millimeters, and
It is placed on the solid culture base table for being coated with Gram-negative bacteria Escherichia coli and gram-positive bacteria staphylococcus aureus bacterium solution
On face, observe the antiseptic dressing of step 4 preparation to Escherichia coli after cultivating 24 hours in 37 DEG C of constant incubators
Inhibition zone is 8.6 millimeters, and the inhibition zone to staphylococcus aureus is 10.2 millimeters, shows the antiseptic dressing tool of this method preparation
There is good antibiotic property.
Embodiment 2:
Step 1: weigh 1.0 grams of polyvinyl butyrals (PVB, molecular weight 90000), be all dissolved in 9.0 grams it is anhydrous
In ethyl alcohol, magnetic agitation 8 hours to being completely dissolved at room temperature, obtains the uniform PVB solution that Solute mass fraction is 10wt%；
Step 2: weighing 2.0 grams of coptis extracts and be distributed in 8.0 grams of dehydrated alcohols, magnetic agitation 8 hours is extremely at room temperature
It is evenly dispersed, obtain the uniform suspension that Solute mass fraction is 20wt%；
Step 3: coptis suspension obtained in step 2 being added in PVB solution prepared by step 1, the amount of addition makes
The mass percentage of the coptis is 5% in solution, is stirred 6 hours, it is uniformly mixed, then is made after standing 12 hours uniformly steady
Fixed spinning precursor liquid；
Step 4: spinning precursor liquid prepared in step 3 is added in 5ml syringe (1.0 millimeters of needle diameter),
And be installed on portable electrostatic spinning apparatus, it turns on the power switch, adjustment spinning voltage is 12 kilovolts, and fltting speed is 2 milliliters
Per hour, control spinning distance carries out spinning under conditions of being 10 centimetres from wound, directly deposits the PVB/ coptis in wound
Micro nanometer fiber antiseptic dressing, for obtained PVB/ coptis nano fibrous membrane with a thickness of 150 microns, fibre diameter is about 900 nanometers,
Its pattern is as shown in Figure 2 under scanning electron microscope.
It is 55 degree with the contact angle of the PVB/ coptis tunica fibrosa prepared in simulated body fluid SBF testing procedure 4, shows electricity in situ
The standby PVB/ coptis is spinned with good wellability.In testing procedure 4 gas permeability of PVB/ coptis tunica fibrosa be 94.3 ±
8.46 squares of metre per second (m/s)s are 7 times higher than the gas permeability of traditional adhesive bandage.The PVB/ coptis nanofiber prepared in step 4 is put
It sets on the aseptic filter paper that diameter is 5 millimeters, and is placed on and is coated with Gram-negative bacteria Escherichia coli and gram-positive bacteria gold
It is big that inhibition zone is observed on the solid culture primary surface of staphylococcus aureus bacterium solution, after cultivating 24 hours in 37 DEG C of constant incubators
Antiseptic dressing prepared by small reflection antibacterial situation discovery step 4 is 9.4 millimeters to the inhibition zone of Escherichia coli, to golden yellow grape
The inhibition zone of coccus is 10.2 millimeters, shows that the antiseptic dressing of this method preparation has good antibiotic property.
Embodiment 3:
Step 1: weigh 1.5 grams of zeins, be all dissolved in 8.5 grams of concentration be 80% alcohol (dehydrated alcohol: go from
Sub- water=4:1) in, magnetic agitation 10 hours to being completely dissolved at room temperature, obtains the corn egg that Solute mass fraction is 15wt%
White solution；
Step 2: weighing 2.0 grams of Honegsukle flower P.Es and be dissolved into 8.0 grams of deionized waters, at room temperature magnetic agitation 8 hours
It is extremely evenly dispersed, obtain the homogeneous solution that Solute mass fraction is 20wt%；
Step 3: honeysuckle solution obtained in step 2 is added in corn protein solution prepared by step 1, addition
Amount makes the mass percentage 3% of GOLD FROM PLATING SOLUTION honeysuckle flower, stirs 6 hours, is uniformly mixed it, then is made after standing 12 hours
Uniform and stable spinning precursor liquid；
Step 4: spinning precursor liquid prepared in step 3 is added in 5ml syringe (0.8 millimeter of needle diameter),
And be installed on electrostatic spinning apparatus in situ, it turns on the power switch, adjustment spinning voltage is 15 kilovolts, and fltting speed is 3 milliliters
Per hour, air velocity is 12 ls/h, and control spinning distance carries out spinning under conditions of being 12 centimetres from wound, directly
Zein/honeysuckle micro nanometer fiber antiseptic dressing is deposited in wound, obtained zein/honeysuckle fibre diameter is about
It is 1200 nanometers, pattern is as shown in Figure 3 under scanning electron microscope.
It is 15 degree with the zein prepared in simulated body fluid SBF testing procedure 4/honeysuckle tunica fibrosa contact angle, table
Zein/honeysuckle of bright original position electrospinning preparation has good wellability.Zein/honeysuckle is fine in testing procedure 4
The gas permeability for tieing up film is 77.63 ± 11.61 squares of meter per seconds, 5 times higher than the gas permeability of traditional adhesive bandage.It will be prepared in step 4
Zein/honeysuckle tunica fibrosa be placed on diameter and be on 5 millimeters of aseptic filter paper, and be placed on and be coated with Gram-negative bacteria
On the solid culture primary surface of Escherichia coli and gram-positive bacteria staphylococcus aureus bacterium solution, in 37 DEG C of constant incubators
The antiseptic dressing that after culture 24 hours prepared by observation inhibition zone size reflection antibacterial situation discovery step 4 is to the antibacterial of Escherichia coli
Circle is 10.4 millimeters, and the inhibition zone to staphylococcus aureus is 11.2 millimeters, shows that the antiseptic dressing of this method preparation has
Good antibiotic property.
Embodiment enumerated above is not to technical solution described in the invention only for understanding that the present invention is used
Restriction, the those of ordinary skill in relation to field on the basis of the technical solution described in claim, can also make a variety of changes
Change or deformation, all equivalent variations or deformation should all be covered within the scope of the claims of the present invention.The present invention is not
Place is described in detail, is the well-known technique of those skilled in the art of the present technique.
Claims (8)
1. a kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing be prepared in situ and application method, comprising the following steps:
Step 1: polymeric being completely dissolved with dehydrated alcohol, Polymer Solution is made；
Step 2: Chinese herbal medicine extract being dissolved into deionized water or is added to height prepared by step 1 after being distributed in dehydrated alcohol
It is the 1-5wt% of polymer content to the quality of Chinese herbal medicine extract in molecular solution, stirring is to obtaining spinning after substantially uniformity
Precursor liquid；
Step 3: spinning precursor liquid prepared in step 2 being added in syringe, and syringe is put into portable electrostatic
In device for spinning, equipment is opened, adjusts spinning parameter to there is fiber uniformly to spray, nozzle mouth is controlled and receives the interval distance of wound
From, it can be achieved that nanofiber antiseptic dressing is prepared in situ in wound.
2. a kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing as described in claim 1 be prepared in situ and application method,
It is characterized in that, the polymeric is polyethylene Topiramate Los oxazolidinone PVP, polyvinyl butyral PVB and zein
Powder, Polymer Solution concentration are 8-15wt%.
3. a kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing as described in claim 1 be prepared in situ and application method,
Be characterized in that, the Chinese herbal medicine extract be isatis root extract, Folium Isatidis extract, Honegsukle flower P.E, coptis extract,
Baical Skullcap root P.E, forsythia suspense extraction etc..
4. a kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing as described in claim 1 be prepared in situ and application method,
It is characterized in that, the syringe is 5-10ml injector for medical purpose, and it is the flattened metal needle of 0.3-1.5mm that syringe needle, which selects internal diameter,
Head.
5. a kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing as described in claim 1 be prepared in situ and application method, institute
The portable electric spinning equipment stated is hand-held electric spinning equipment or the electric spinning equipment in situ with hand-held spray head.
6. a kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing as described in claim 1 be prepared in situ and application method, institute
The spinning parameter stated is spinning voltage 8-15 kilovolts, 5-12 centimetres of spinning distance.
7. a kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing as described in claim 1 be prepared in situ and application method, institute
The fiber dressing stated is that direct electrospinning deposits to wound, directly contacts wound without hand.
8. a kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing as claimed in claim 7 be prepared in situ and application method, institute
The fiber dressing stated with a thickness of 80-1000 microns.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111035792A (en) * | 2019-12-13 | 2020-04-21 | 青岛农业大学 | Bergenin electrospun membrane callus medical dressing and preparation method and application thereof |
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| CN113786512A (en) * | 2021-09-24 | 2021-12-14 | 华南理工大学 | Low-pressure in-situ anti-bacteria repair-promoting electrospinning dressing and preparation method thereof |
| CN114150442A (en) * | 2021-12-09 | 2022-03-08 | 吉林大学 | Garlicin-loaded antibacterial packaging film and preparation method thereof |
| CN115418796A (en) * | 2022-09-14 | 2022-12-02 | 百达联康生物科技(深圳)有限公司 | Antibacterial fiber membrane and preparation method thereof |
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