CN107158447A - A kind of antibacterial zeins dressing with controllable orientation and preparation method thereof - Google Patents

A kind of antibacterial zeins dressing with controllable orientation and preparation method thereof Download PDF

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Publication number
CN107158447A
CN107158447A CN201710546579.5A CN201710546579A CN107158447A CN 107158447 A CN107158447 A CN 107158447A CN 201710546579 A CN201710546579 A CN 201710546579A CN 107158447 A CN107158447 A CN 107158447A
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zeins
dressing
layer
antibacterial
fiber
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CN107158447B (en
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严玉蓉
杨志鹏
缪艳娟
刘志鹏
陈洁娜
杨军龙
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South China University of Technology SCUT
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South China University of Technology SCUT
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/32Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42Use of materials characterised by their function or physical properties
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42Use of materials characterised by their function or physical properties
    • A61L15/425Porous materials, e.g. foams or sponges
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42Use of materials characterised by their function or physical properties
    • A61L15/44Medicaments
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/21Acids
    • A61L2300/214Amino acids
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/216Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with other specific functional groups, e.g. aldehydes, ketones, phenols, quaternary phosphonium groups
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/22Lipids, fatty acids, e.g. prostaglandins, oils, fats, waxes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/30Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404Biocides, antimicrobial agents, antiseptic agents

Abstract

The invention belongs to medical material tech field, a kind of antibacterial zeins dressing with controllable orientation and preparation method thereof is disclosed.The preparation method is:Zein solution containing antimicrobial component is passed through into electrospinning process, fiber dressing layer is made using the net template with regularly arranged geometric figure as receiver respectively, with surface there is the roller receiver of regularly arranged striped bulge-structure to receive and obtain fiber guide layer;By fiber dressing layer and fiber guide layer superimposion, zeins tunica fibrosa functional layer is obtained;Then zeins tunica fibrosa functional layer is combined with spun laced fabric supporting layer and polypropylene film barrier layer, obtains the antibacterial zeins dressing with controllable orientation.The tunica fibrosa functional layer of the dressing of the present invention has microcellular structure and flow-guiding structure, with good permeability or gas permeability, can realize directional water guiding and avoid bleeding back, so as to keep the dry and comfortable of wound.

Description

A kind of antibacterial zeins dressing with controllable orientation and preparation method thereof
Technical field
The invention belongs to medical material tech field, and in particular to a kind of antibacterial zeins with controllable orientation Dressing and preparation method thereof.
Background technology
Zeins (zein) is a kind of natural polymer polymer, is the topmost storage protein of corn, easily obtains , it is economic and environment-friendly.Zeins has good biocompatibility, biodegradability etc., is a kind of environment-friendly, green The biomaterial of color, safety.
Substantial amounts of hydrophobic amino acid is not only there is in zeins molecule, also containing more sulfur-bearing amino Acid, with strong hydrophobicity.In polar environment, hydrophilic radical exposes in zeins molecule, and hydrophobic grouping is embedded, Stable micellar structure can be formed.Alcohol soluble protein can dissolve in certain solvent, therefore can prepare solution, prepare film forming or Fibrous material, insoluble cross-linked corn alcohol-soluble protein film or fibre just can be obtained after being further crosslinked using aldehydes Dimension.Zeins easily combines to form polyblend with other compounds, and can be built according to different demands has difference The vector delivery system of characteristic.Therefore zeins can be as the good of the liquid conductive nanofiber for preparing controllable orientation Good raw material.
Plant extraction liquid antiseptic is plant then evolves generation to adapt to environment, and certain plants extract solution is directed to certain There is certain control or suppression, elimination to act on for a kind of illness or some bacteriums, virus etc..For example:Compound plant is carried Take liquid good for the inhibition of Aeromonas hydrophila, and the method extracted and separated is, it is known that operate simple and easy to do.It is existing Nowadays, the research for extracting antiseptic from plant is more and more, therefore, will possess bigger when selecting and using Operating space.
Plant extraction liquid antiseptic is not likely to produce drug resistance, will not Induction of bacterial virus etc. be evolved into and be more difficult to resist and disappear Remove, solve current puzzlement people's synthetic antibacterial agents resistance problems.In addition, relative to artificial synthesized antiseptic, plant extract Liquid antiseptic is more naturally environmentally friendly, meets current era people for natural health, the sustainable pursuit of environmental protection.
Electrostatic spinning technique (electrospinning technology) is a kind of to prepare the new of monodimension nanometer material Technology.Early in 1934, Formhals just applied for the experimental provision on preparing polymer fiber using high-voltage electrostatic field Patent.Afterwards, due to low production efficiency the problems such as, causes the development of the technology more slow always.Until 90 years 20th century Generation, the rise because of nano science and the Darrell in Akron university of the U.S. (Universityof Akron) H.Reneker teaches seminar, the Gregory of Massachusetts science and engineering (Massachusetts Institute ofTechnology) Under the promotion of the seminars such as C.Rutledge, electrostatic spinning technique is just rapidly developed.The letter of electrostatic spinning manufacturing equipment structure Single, cost of spinning is low, can prepare compound, hollow or solid nanofiber of continuous organic and inorganic, organic/inorganic etc., receive and grind The extensive concern for the person of studying carefully.In recent ten years, researchers develop coaxial electrostatic spinning technology by improving needle head structure and are used for Doughnut/nanotube is prepared, or by improving reception device, obtains a series of different patterning Nanowire of accumulation modes Structure is tieed up, such as:Along the ANFs of the axial proper alignment of fiber, the nanofiber being axially arranged vertically with radial direction etc..It is height-oriented and Regularly arranged nanofiber is due to special mechanics, electrical and optical properties, receiving the very big concern of researcher.
Electrostatic spinning can realize that template Shape design general principle is the point discharge using charge concentration and supporting body Effect, wherein formwork structure are designed, and material obtains effect to specific morphology direct influence.The shape of template is received by changing Shape, material character and the motion state for receiving template, can obtain the nanofiber felted material of various accumulation shapes.
The content of the invention
Based on above prior art, primary and foremost purpose of the invention is to provide a kind of antibacterial corn alcohol with controllable orientation The preparation method of molten albumen dressing.
Another object of the present invention is to provide a kind of antibacterial with controllable orientation prepared by the above method Zeins dressing.
The object of the invention is achieved through the following technical solutions:
A kind of preparation method of the antibacterial zeins dressing with controllable orientation, including following preparation process:
(1) zeins is dissolved in solvent, obtains uniform zein solution, then added antibacterial and live Property composition, is uniformly mixed rear standing and defoaming, obtains the zein solution containing antimicrobial component;
(2) by the zein solution containing antimicrobial component by electrospinning process, respectively with rule row Fiber dressing layer is made as receiver in the net template of the geometric figure of row, has regularly arranged striped bulge-structure with surface Roller receiver receive obtain fiber guide layer;By fiber dressing layer and fiber guide layer superimposion, corn alcohol is obtained molten Azelon film functional layer;
(3) zeins tunica fibrosa functional layer is combined with spun laced fabric supporting layer and polypropylene film barrier layer, by Fiber dressing layer, fiber guide layer, spun laced fabric supporting layer and polyethylene film barrier layer are up to followed successively by down, are obtained with controllable The antibacterial zeins dressing of orientation.
Preferably, the solvent described in step (1) refers to that ethanol or glacial acetic acid that mass concentration is 40%~98% are water-soluble Liquid.
Preferably, described in step (1) in zein solution the mass concentration of zeins for 5%~ 40%.
Preferably, the Antibacterial Constituents include but is not limited to the antibacterial activity based on microorganism or plant extract into Point;Polylysine, Paeonol, Tea Polyphenols, eucalyptus oil, Rosa Damascana, aloe extract, honeysuckle is included but are not limited to extract The natural antibacterial extracts such as thing, baicalin, ginkgo antibacterial protein, lemon grass (Cymbopogon citratus) essential oil, water chestnut bark extract.
Preferably, the addition of the Antibacterial Constituents is the 0.01%~5% of zeins quality.
Preferably, the net template with regularly arranged geometric figure is hexagon, rectangle, rhombus, square Or circular net template, sizing grid is 0.1~3mm, and density is 2~40/cm2, thickness is 0.1~2.0mm.
Preferably, there is the roller receiver of regularly arranged striped bulge-structure to be a diameter of 8~50cm on the surface, long Spend the cylindrical roller for 10~90cm;The width of fringe d of cylinder surface striped bulge-structure is 0.3~1cm, and density is 1~5 Individual/cm (i.e. adjacent stripes bulge-structure at intervals of 0.2~1cm), rising height h are that 0.2~1cm, protrusion angle α are 100 ~150 °.Its structural representation is as shown in Figure 1.
Preferably, the material of the fiber dressing layer receiver includes plastics, ceramics, metal or alloy;The roller connects Receiving the material of device includes metal or alloy.
Preferably, the method for the electrostatic spinning includes syringe needle electrostatic spinning, slit electrostatic spinning, needle-less Free Surface Line electrode electrostatic spinning, needle-less Free Surface roller electrode electrostatic spinning, needle-less Free Surface shuttle electrode electrostatic spinning, needleless Head Free Surface spiral line electrode electrostatic spinning or centrifugation electrostatic spinning etc..
Preferably, fibre diameter is 50~2000nm in the zeins tunica fibrosa functional layer.
A kind of antibacterial zeins dressing with controllable orientation, is prepared by the above method.
The principle of the invention is:There is the net template of geometric figure of regular arrangement using surface as receiver, figure Arrangement have impact on the spatial arrangement of electrostatic field, grid hollow sectors electric field can weaken, and fiber deposition is less, so that tunica fibrosa Structure it is similar to the figure of template, be covered with substantial amounts of microcellular structure, increase the gas permeability of dressing;Using surface there is rule to arrange The roller receiver of row striped bulge-structure prepares fiber guide layer, and the roller of rotation pulls fiber so that fiber in circumference or The raised structures that person is upwardly formed on orientation texture, roller along polygon corner angle side cause zeins tunica fibrosa to obtain The fiber groove of complementary structure, zeins Hydrophilic Fiber is poor, and liquid is diffused into after guide layer, along fiber-wall-element model Direction is flowed, and collects in fiber groove, and this causes sample to have certain interim liquid storage capacity, while the liquid collected is recessed Flowed in groove to external diffusion, prevent liquid to be back to again in wound, keep wound dry and comfortable.
The preparation method and resulting dressing of the present invention has the following advantages that and beneficial effect:
(1) the antibacterial zeins dressing with controllable orientation of the invention adds natural antibacterial active component, its Good anti-bacterial effect and have no toxic side effect, more than 99% is reached to the bacteriostasis rate of gram-positive bacteria and Gram-negative bacteria.
(2) the antibacterial zeins dressing with controllable orientation of the invention using have good biocompatibility, The zeins of biodegradability is material of main part, environmentally friendly and economic and environment-friendly.
(3) present invention obtains composite membrane using electrostatic spinning or centrifugal spinning technology, and gained composite membrane has high porosity And bigger serface, and its fibre structure has good similitude with extracellular matrix, available for skin injury or operation Skin afterwards recovers the dressing with regeneration.
(4) microcellular structure has well to air and vapor in the fiber dressing layer that preparation method of the invention is obtained Permeability or gas permeability, but there is higher resistance to hydrostatic pressure to the infiltration of aqueous water, i.e., this kind ventilating structure includes many energy From a surface to the opening or passage on another surface, its hole is sized so that air and water vapour molecule by film, and There is good resistance to liquid water molecules;And because the addition of one or more antiseptics causes with good antibacterial work Property, antiseptic can be released under certain condition, may be used as dressing materials.
(5) dressing of the invention is from fiber dressing layer to spun laced fabric supporting layer, and the moisture pick-up properties of dressing gradually strengthens, and is formed Good leads wet gradient.In the presence of differential capillary effect, directional water guiding is realized.With the guide layer dressing energy being well orientated It is enough that a stable balance is reached between infiltration and diffusion.When wound fluid or wound purulence thing are arrived through tunica fibrosa functional layer During up to guide layer, because guide layer has certain thickness fluff structure and the distribution of larger longitudinal fiber, thus diffusate or Purulence thing can quickly be captured and along longitudinal diffusion, so that liquid is slow and effectively enters guide layer, and temporarily will Fluid preservation, it is to avoid liquid keeps wound dry and comfortable not by bleeding back for absorbing and cause in time.
Brief description of the drawings
Fig. 1 is the structural representation of cylinder surface striped bulge-structure of the present invention;
Fig. 2 is the stepped construction schematic diagram of dressing obtained by the embodiment of the present invention;
Fig. 3 is the structural representation of fiber dressing layer and fiber guide layer in the gained dressing of the embodiment of the present invention 1;
Fig. 4 is the structural representation of fiber dressing layer and fiber guide layer in the gained dressing of the embodiment of the present invention 2;
Fig. 5 is the structural representation of fiber dressing layer and fiber guide layer in the gained dressing of the embodiment of the present invention 3;
Numbering is described as follows in figure:1- polypropylene film barrier layers, 2- spun laced fabric supporting layers, 3- fiber guide layers, 4- is fine Tie up dressing layer.
Embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited In this.
Embodiment 1
Take the last particle of the pure zein powder of analysis, from mass concentration for 40% ethanol solution as solvent, often Temperature stirring 30min, obtains mass concentration for 5% uniform zein solution;Treat that zein solution is cooled to After room temperature, then weigh in a certain amount of aloe antibiotic extract addition zein solution, stirring at normal temperature 1h obtains aloe Extract quality percentage composition is 0.1% zeins-plants antimicrobial extract solution, standing and defoaming 1h.It will prepare Mixed solution zeins-plant extract fibres guide layer is prepared using Free Surface line electrode electrospinning process And fiber dressing layer.Under 65kV voltage, zeins-plants antimicrobial extract solution is in the rotating speed of line electrode Carry out electrospinning under the conditions of 10r/min, and away from being received at syringe needle about 10cm, be that 90cm, rotating speed are from a diameter of 50cm, length 1200r/min's, surface have width be 1cm, density be 5/cm, be highly 1cm, the straight line metal that protrusion angle is 120 ° Raised roller receiver, which is received, can obtain fiber guide layer;From surface there is size to be that 0.5mm, density are 40/cm2, thickness Fiber dressing layer can be obtained by being received for 0.1mm roundness mess templates receiver, the structure of gained fiber dressing layer and fiber guide layer Schematic diagram is as shown in Figure 3.By fiber dressing layer and fiber guide layer superimposion, zeins tunica fibrosa function is obtained Layer.Fibre diameter is 900~1200nm in gained zeins tunica fibrosa functional layer.By zeins tunica fibrosa work( Ergosphere and spun laced fabric supporting layer and polypropylene film barrier layer are compound, be from top to bottom followed successively by fiber dressing layer, fiber guide layer, Spun laced fabric supporting layer and polypropylene film barrier layer, obtain the antibacterial zeins dressing with controllable orientation.Gained is applied The stepped construction schematic diagram of material is as shown in Figure 2.
The antibiotic rate test of antibacterial zeins dressing obtained by the present embodiment:
Take (10 ± 0.1) ml agar in each sterile petri dish, allow agar solidified.Take requirement agar, heating water bath to (45 ±1)℃.It is (1-5 × 10 by bacterial concentration8Cfu/ml microbionation) makes bacterium on 150ml agar with forced oscillation container It is dispersed.(5 ± 0.1) ml is on culture dish, allows agar solidification.Using the nutrition fine jade in inoculation time one hour during experiment Fat culture dish.According to EN ISO 20645:2004 methods are operated.Pressed with sterilizing tweezers in the middle of culture and pave certain area Antibacterial zeins dressing, fiber dressing layer and bacterium is had good contact, at (37 ± 1) DEG C after culture 18h to 24h Count plate.According to EN ISO 20645:2004 methods determine viable count, obtain the present embodiment antibacterial zeins dressing Antibiotic rate be 99.67%.
Embodiment 2
Take the last particle of the pure zein powder of analysis, from mass concentration for 65% ethanol solution as solvent, often Temperature stirring 30min, obtains mass concentration for 18% uniform zein solution;Treat that zein solution is cooled to After room temperature, then weigh in a certain amount of Paeonol addition zein solution, stirring at normal temperature 1h obtains Paeonol quality hundred Divide zeins-plants antimicrobial extract solution that content is 0.4%, standing and defoaming 1h.The mixed solution prepared is adopted Zeins-plant extract fibres guide layer and fiber dressing layer are prepared with syringe needle electrospinning process.9kV's Under voltage, zeins-plants antimicrobial extract solution carries out electrospinning with the flow velocity in 0.4ml/h/ holes, and away from syringe needle about Received at 10cm, be that 60cm, rotating speed are 1300r/min from a diameter of 9cm, length, surface has width for 0.5cm, close Spend for 5/cm, be highly 0.2cm, the roller receiver for the straight line metal bump that protrusion angle is 120 ° receives and can obtain fiber and lead Fluid layer;From surface there is size to be that 1mm, density are 30/cm2, thickness be 0.5mm square net templates receiver receive Fiber dressing layer can be obtained, the structural representation of gained fiber dressing layer and fiber guide layer is as shown in Figure 4.By fiber dressing layer with Fiber guide layer superimposion, obtains zeins tunica fibrosa functional layer.Gained zeins tunica fibrosa functional layer Middle fibre diameter is about 800~1500nm.Zeins tunica fibrosa functional layer and spun laced fabric supporting layer and polypropylene is thin Film barrier layer is combined, and is from top to bottom followed successively by fiber dressing layer, fiber guide layer, spun laced fabric supporting layer and polypropylene film barrier Layer, obtains the antibacterial zeins dressing with controllable orientation.The stepped construction schematic diagram of gained dressing is as shown in Figure 2.
Antibiotic rate is 99.95% after tested for antibacterial zeins dressing obtained by the present embodiment.
Embodiment 3
The last particle of the pure zein powder of analysis is taken, from the glacial acetic acid solution that mass concentration is 90% as solvent, Stirring at normal temperature 30min, obtains mass concentration for 19% uniform zein solution;Treat that zein solution is cooled down To after room temperature, then weigh in a certain amount of eucalyptus oil addition zein solution, stirring at normal temperature 1h obtains eucalyptus oil quality Percentage composition is 0.01% zeins-plants antimicrobial extract solution, standing and defoaming 1h.By the mixed solution prepared Using needle-less Free Surface roller electrode electrospinning process prepare zeins-plant extract fibres guide layer and Fiber dressing layer.Under 70kV voltage, zeins-plants antimicrobial extract solution is carried out with 0.4ml/h flow velocity Electrospinning, and be that 10cm, rotating speed are 1500r/min, surface from a diameter of 40cm, length away from being received at syringe needle about 10cm With width be 0.3cm, density be 1/cm, be highly 0.5cm, the roller for the straight line metal bump that protrusion angle is 150 ° connects Fiber guide layer can be obtained by receiving device reception;From surface there is size to be that 0.5mm, density are 30/cm2, thickness be 10mm positive six Side shape net template receiver, which is received, can obtain fiber dressing layer, and the structural representation of gained fiber dressing layer and fiber guide layer is such as Shown in Fig. 5.By fiber dressing layer and fiber guide layer superimposion, zeins tunica fibrosa functional layer is obtained.Gained is beautiful Fibre diameter is 1200~2000nm in rice alcohol soluble protein tunica fibrosa functional layer.By zeins tunica fibrosa functional layer and water Thorn cloth supporting layer and polypropylene film barrier layer are compound, are from top to bottom followed successively by fiber dressing layer, fiber guide layer, spun laced fabric branch Layer and polypropylene film barrier layer are supportted, the antibacterial zeins dressing with controllable orientation is obtained.The stacking of gained dressing Structural representation is as shown in Figure 2.
Antibiotic rate is 99.69% after tested for antibacterial zeins dressing obtained by the present embodiment.
Embodiment 4
The last particle of the pure zein powder of analysis is taken, from the glacial acetic acid solution that mass concentration is 98% as solvent, Stirring at normal temperature 30min, obtains mass concentration for 20% uniform zein solution;Treat that zein solution is cooled down To after room temperature, then weigh in a certain amount of Tea Polyphenols addition zein solution, stirring at normal temperature 1h obtains Tea Polyphenols quality Percentage composition is 5% zeins-plants antimicrobial extract solution, standing and defoaming 1h.The mixed solution prepared is adopted Prepared with needle-less Free Surface screw electrode electrospinning process zeins-plant extract fibres guide layer and Fiber dressing layer.Under 60kV voltage, zeins-plants antimicrobial extract solution is carried out with 1.2ml/h flow velocity Electrospinning, and be that 60cm, rotating speed are 1600r/min, surface from a diameter of 13cm, length away from being received at syringe needle about 10cm With width be 1cm, density be 2/cm, be highly 1cm, the roller receiver for the straight line metal bump that protrusion angle is 100 ° Reception can obtain fiber guide layer;From surface there is size to be that 1mm, density are 40/cm2, thickness be 5mm roundness mess templates Receiver, which is received, can obtain fiber dressing layer, by fiber dressing layer and fiber guide layer superimposion, obtain zeins fine Tie up film functional layer.Fibre diameter is 700~1300nm in gained zeins tunica fibrosa functional layer.By zeins Tunica fibrosa functional layer and spun laced fabric supporting layer and polypropylene film barrier layer are compound, are from top to bottom followed successively by fiber dressing layer, fibre Guide layer, spun laced fabric supporting layer and polypropylene film barrier layer are tieed up, the antibacterial zeins with controllable orientation is obtained and applies Material.The stepped construction schematic diagram of gained dressing is as shown in Figure 2.
Antibiotic rate is 99.99% after tested for antibacterial zeins dressing obtained by the present embodiment.
Embodiment 5
Take the last particle of the pure zein powder of analysis, from mass concentration for 98% ethanol solution as solvent, often Temperature stirring 30min, obtains mass concentration for 40% uniform zein solution;Treat that zein solution is cooled to After room temperature, then weigh in a certain amount of polylysine addition zein solution, stirring at normal temperature 1h obtains polylysine matter Measure zeins-plants antimicrobial extract solution that percentage composition is 3%, standing and defoaming 1h.By the mixed solution prepared Zeins-plant extract fibres guide layer and fiber dressing layer are prepared using centrifugation electrospinning process. Under 35kV voltage, zeins-plants antimicrobial extract solution carries out electrospinning with 2.5ml/h flow velocity, centrifuges electrostatic Spinning centrifugation disc spin speed be 300r/min, and away from syringe needle about 10cm at receive, from a diameter of 8cm, length be 50cm, Rotating speed for 1500r/min, surface have width be 0.8cm, density be 1/cm, be highly 1cm, protrusion angle is 110 ° The roller receiver of straight line metal bump, which is received, can obtain fiber guide layer;From surface have size be 3mm, density be 2/ cm2, thickness be that 20mm roundness mess templates receiver receives and can obtain fiber dressing layer, fiber dressing layer and fiber water conservancy diversion are laminated Plus it is compound, obtain zeins tunica fibrosa functional layer.Fibre diameter is in gained zeins tunica fibrosa functional layer 1000~1500nm.Zeins tunica fibrosa functional layer is combined with spun laced fabric supporting layer and polypropylene film barrier layer, From top to bottom be followed successively by fiber dressing layer, fiber guide layer, spun laced fabric supporting layer and polypropylene film barrier layer, obtain have can Control the antibacterial zeins dressing of orientation.The stepped construction schematic diagram of gained dressing is as shown in Figure 2.
Antibiotic rate is 99.98% after tested for antibacterial zeins dressing obtained by the present embodiment.
Embodiment 6
The last particle of the pure zein powder of analysis is taken, from the glacial acetic acid solution that mass concentration is 90% as solvent, Stirring at normal temperature 30min, obtains mass concentration for 25% uniform zein solution;Treat that zein solution is cooled down To after room temperature, then weigh in a certain amount of aloe antibiotic extract addition zein solution, stirring at normal temperature 1h obtains reed Luxuriant growth antimicrobial extract weight/mass percentage composition is 2% zeins-plants antimicrobial extract solution, standing and defoaming 1h.Will The mixed solution prepared prepares zeins-plant extracts using needle-less Free Surface shuttle electrode electrospinning process Fiber guide layer and fiber dressing layer.Under 10kV voltage, zeins-plants antimicrobial extract solution with 3.0ml/h flow velocity carries out electrospinning, and away from being received at syringe needle about 10cm, is from a diameter of 12cm, a diameter of 80cm, rotating speed 1400r/min's, surface have width be 0.3cm, density be 2/cm, be highly 0.4cm, the straight line that protrusion angle is 125 ° The roller receiver of metal bump, which is received, can obtain fiber guide layer;From surface there is size to be that 2mm, density are 20/cm2、 Thickness is that the reception of 8mm network templates receiver can obtain fiber dressing layer, fiber dressing layer is superimposed with fiber guide layer multiple Close, obtain zeins tunica fibrosa functional layer.Fibre diameter is 1000 in gained zeins tunica fibrosa functional layer ~1200nm.Zeins tunica fibrosa functional layer is combined with spun laced fabric supporting layer and polypropylene film barrier layer, by upper Fiber dressing layer, fiber guide layer, spun laced fabric supporting layer and polypropylene film barrier layer are followed successively by under, obtains taking with controllable To antibacterial zeins dressing.The stepped construction schematic diagram of gained dressing is as shown in Figure 2.
Antibiotic rate is 99.93% after tested for antibacterial zeins dressing obtained by the present embodiment.
Embodiment 7
Take the last particle of the pure zein powder of analysis, from mass concentration for 95% ethanol solution as solvent, often Temperature stirring 30min, obtains mass concentration for 25% uniform zein solution;Treat that zein solution is cooled to After room temperature, then weigh a certain amount of aloe and by the oily (mass ratio 1 of tree:2) composite antibacterial extract addition zeins is molten In liquid, stirring at normal temperature 1h obtains zeins-plants antimicrobial that composite antibacterial extract quality percentage composition is 1.0% Extract solution, standing and defoaming 1h.The mixed solution prepared is prepared into zeins-plant using syringe needle electrospinning process Thing extracts fibres guide layer and fiber dressing layer.Under 13kV voltage, zeins-plants antimicrobial extract is molten Liquid carries out electrospinning with the flow velocity in 0.4ml/h/ holes, and away from being received at syringe needle about 10cm, is from a diameter of 20cm, length 60cm, rotating speed for 1800r/min, surface have width be 1cm, density be 1/cm, be highly 0.5cm, protrusion angle is The roller receiver of 135 ° of straight line metal bump, which is received, can obtain fiber guide layer;From surface there is size to be that 1mm, density are 30/cm2, thickness be 0.5mm square net templates receiver receive can obtain fiber dressing layer, by fiber dressing layer and fiber Guide layer superimposion, obtains zeins tunica fibrosa functional layer.It is fine in gained zeins tunica fibrosa functional layer Tie up a diameter of 50~1000nm.By zeins tunica fibrosa functional layer and spun laced fabric supporting layer and polypropylene film barrier layer It is compound, fiber dressing layer, fiber guide layer, spun laced fabric supporting layer and polypropylene film barrier layer are from top to bottom followed successively by, is obtained Antibacterial zeins dressing with controllable orientation.The stepped construction schematic diagram of gained dressing is as shown in Figure 2.
Antibiotic rate is 99.99% after tested for antibacterial zeins dressing obtained by the present embodiment.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification, Equivalent substitute mode is should be, is included within protection scope of the present invention.

Claims (10)

1. a kind of preparation method of the antibacterial zeins dressing with controllable orientation, it is characterised in that including following preparation Step:
(1) zeins is dissolved in solvent, obtains uniform zein solution, then add antibacterial activity into Point, rear standing and defoaming is uniformly mixed, the zein solution containing antimicrobial component is obtained;
(2) by the zein solution containing antimicrobial component by electrospinning process, respectively with regularly arranged Fiber dressing layer is made as receiver in the net template of geometric figure, has the rolling of regularly arranged striped bulge-structure with surface Cylinder receiver, which is received, obtains fiber guide layer;By fiber dressing layer and fiber guide layer superimposion, zeins is obtained Tunica fibrosa functional layer;
(3) zeins tunica fibrosa functional layer is combined with spun laced fabric supporting layer and polypropylene film barrier layer, by up to Under be followed successively by fiber dressing layer, fiber guide layer, spun laced fabric supporting layer and polyethylene film barrier layer, obtain with controllable orientation Antibacterial zeins dressing.
2. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its It is characterised by:Solvent described in step (1) refers to the ethanol or glacial acetic acid aqueous solution that mass concentration is 40%~98%.
3. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its It is characterised by:The mass concentration of zeins is 5%~40% in zein solution described in step (1).
4. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its It is characterised by:The Antibacterial Constituents include polylysine, Paeonol, Tea Polyphenols, eucalyptus oil, Rosa Damascana, aloe and extracted Thing, Honegsukle flower P.E, baicalin, ginkgo antibacterial protein, lemon grass (Cymbopogon citratus) essential oil or water chestnut bark extract.
5. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its It is characterised by:The addition of the Antibacterial Constituents is the 0.1%~5% of zein solution quality.
6. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its It is characterised by:The net template with regularly arranged geometric figure is the net template of hexagon, square or circle, Sizing grid is 0.1~3mm, and density is 2~40/cm2, thickness is 0.1~2.0mm.
7. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its It is characterised by:There is the roller receiver of regularly arranged striped bulge-structure to be a diameter of 8~15cm on the surface, and length is 15 ~30cm cylindrical roller;The width of fringe of the striped bulge-structure of cylinder surface be 0.3~1cm, density be 1~5/cm, Rising height is that 0.2~1cm, protrusion angle are 100~150 °.
8. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its It is characterised by:The method of the electrostatic spinning includes single needle electrostatic spinning, slit electrostatic spinning, needle-less Free Surface upper thread electricity Pole electrostatic spinning, needle-less Free Surface roller electrode electrostatic spinning, needle-less Free Surface shuttle electrode electrostatic spinning, needle-less are certainly By perficial helical line electrode electrostatic spinning or centrifugation electrostatic spinning.
9. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its It is characterised by:Fibre diameter is 50~2000nm in the zeins tunica fibrosa functional layer.
10. a kind of antibacterial zeins dressing with controllable orientation, it is characterised in that:It is any by claim 1~9 Method described in is prepared.
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CN110101897A (en) * 2019-04-19 2019-08-09 青岛大学 A kind of method that burn and scald skin is nursed in situ
CN110124094A (en) * 2019-04-19 2019-08-16 青岛大学 A kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing be prepared in situ and application method
CN110306290A (en) * 2019-07-29 2019-10-08 广东工业大学 A kind of preparation method of the anti-oxidant loaded nano tunica fibrosa of edible antibacterial
WO2020077923A1 (en) * 2018-10-19 2020-04-23 中山大学 Polypropylene/tea polyphenol patch for intraperitoneal repair, preparation method therefor and use thereof
CN111850837A (en) * 2020-07-24 2020-10-30 吉林农业大学 Zein-based uniaxial electrostatic spinning oriented fiber film and preparation method thereof
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CN112647193A (en) * 2020-12-31 2021-04-13 上海市第六人民医院 Preparation method of electron beam irradiation crosslinked calcium peroxide-carbon quantum dot @ zein antibacterial film
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WO2020077923A1 (en) * 2018-10-19 2020-04-23 中山大学 Polypropylene/tea polyphenol patch for intraperitoneal repair, preparation method therefor and use thereof
CN110025817A (en) * 2019-04-19 2019-07-19 青岛大学 A kind of preparation and application of zein/plants essential oil composite antibacterial fibre dressing
CN110101897A (en) * 2019-04-19 2019-08-09 青岛大学 A kind of method that burn and scald skin is nursed in situ
CN110124094A (en) * 2019-04-19 2019-08-16 青岛大学 A kind of Chinese herbal medicine based micro-nano fibre antibacterial dressing be prepared in situ and application method
CN110306290A (en) * 2019-07-29 2019-10-08 广东工业大学 A kind of preparation method of the anti-oxidant loaded nano tunica fibrosa of edible antibacterial
CN111850837A (en) * 2020-07-24 2020-10-30 吉林农业大学 Zein-based uniaxial electrostatic spinning oriented fiber film and preparation method thereof
CN112376124A (en) * 2020-11-20 2021-02-19 上海市第六人民医院 Antibacterial dressing
CN112647193A (en) * 2020-12-31 2021-04-13 上海市第六人民医院 Preparation method of electron beam irradiation crosslinked calcium peroxide-carbon quantum dot @ zein antibacterial film
CN112587710A (en) * 2021-02-05 2021-04-02 甘肃省分析测试中心 Preparation process and application of peach kernel extract core-shell nano-structure film
CN113417074A (en) * 2021-05-24 2021-09-21 江苏省农业科学院 Preparation method of antibacterial nanofiber membrane
CN114752091A (en) * 2022-03-25 2022-07-15 中国科学院兰州化学物理研究所 Preparation method of zein/honeysuckle extract composite antibacterial preservative film
CN115531595A (en) * 2022-09-28 2022-12-30 长春工业大学 Antibacterial healing-promoting electrostatic spinning wound dressing and preparation method thereof
CN115531595B (en) * 2022-09-28 2023-08-15 长春工业大学 Antibacterial healing-promoting electrostatic spinning wound dressing and preparation method thereof

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