CN107158447A - A kind of antibacterial zeins dressing with controllable orientation and preparation method thereof - Google Patents
A kind of antibacterial zeins dressing with controllable orientation and preparation method thereof Download PDFInfo
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- CN107158447A CN107158447A CN201710546579.5A CN201710546579A CN107158447A CN 107158447 A CN107158447 A CN 107158447A CN 201710546579 A CN201710546579 A CN 201710546579A CN 107158447 A CN107158447 A CN 107158447A
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- zeins
- dressing
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- antibacterial
- fiber
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- 229920002494 Zein Polymers 0.000 title claims abstract description 118
- 108010055615 Zein Proteins 0.000 title claims abstract description 118
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 54
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 239000010410 layer Substances 0.000 claims abstract description 137
- 239000000835 fiber Substances 0.000 claims abstract description 102
- 239000005019 zein Substances 0.000 claims abstract description 38
- 229940093612 zein Drugs 0.000 claims abstract description 38
- 239000002346 layers by function Substances 0.000 claims abstract description 29
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- 238000000034 method Methods 0.000 claims abstract description 20
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- 239000002904 solvent Substances 0.000 claims description 12
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 10
- UILPJVPSNHJFIK-UHFFFAOYSA-N Paeonol Chemical compound COC1=CC=C(C(C)=O)C(O)=C1 UILPJVPSNHJFIK-UHFFFAOYSA-N 0.000 claims description 8
- 229960000583 acetic acid Drugs 0.000 claims description 5
- 239000012362 glacial acetic acid Substances 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
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- MLIBGOFSXXWRIY-UHFFFAOYSA-N paeonol Natural products COC1=CC=C(O)C(C(C)=O)=C1 MLIBGOFSXXWRIY-UHFFFAOYSA-N 0.000 claims description 4
- 229920000656 polylysine Polymers 0.000 claims description 4
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 4
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- IKIIZLYTISPENI-ZFORQUDYSA-N baicalin Chemical compound O1[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1OC(C(=C1O)O)=CC2=C1C(=O)C=C(C=1C=CC=CC=1)O2 IKIIZLYTISPENI-ZFORQUDYSA-N 0.000 claims description 2
- 229960003321 baicalin Drugs 0.000 claims description 2
- AQHDANHUMGXSJZ-UHFFFAOYSA-N baicalin Natural products OC1C(O)C(C(O)CO)OC1OC(C(=C1O)O)=CC2=C1C(=O)C=C(C=1C=CC=CC=1)O2 AQHDANHUMGXSJZ-UHFFFAOYSA-N 0.000 claims description 2
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- 241000894006 Bacteria Species 0.000 description 4
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- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical group [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
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- 150000001299 aldehydes Chemical class 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/21—Acids
- A61L2300/214—Amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/216—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with other specific functional groups, e.g. aldehydes, ketones, phenols, quaternary phosphonium groups
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/22—Lipids, fatty acids, e.g. prostaglandins, oils, fats, waxes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Epidemiology (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dispersion Chemistry (AREA)
- Artificial Filaments (AREA)
- Nonwoven Fabrics (AREA)
- Spinning Methods And Devices For Manufacturing Artificial Fibers (AREA)
Abstract
The invention belongs to medical material tech field, a kind of antibacterial zeins dressing with controllable orientation and preparation method thereof is disclosed.The preparation method is：Zein solution containing antimicrobial component is passed through into electrospinning process, fiber dressing layer is made using the net template with regularly arranged geometric figure as receiver respectively, with surface there is the roller receiver of regularly arranged striped bulge-structure to receive and obtain fiber guide layer；By fiber dressing layer and fiber guide layer superimposion, zeins tunica fibrosa functional layer is obtained；Then zeins tunica fibrosa functional layer is combined with spun laced fabric supporting layer and polypropylene film barrier layer, obtains the antibacterial zeins dressing with controllable orientation.The tunica fibrosa functional layer of the dressing of the present invention has microcellular structure and flow-guiding structure, with good permeability or gas permeability, can realize directional water guiding and avoid bleeding back, so as to keep the dry and comfortable of wound.
Description
Technical field
The invention belongs to medical material tech field, and in particular to a kind of antibacterial zeins with controllable orientation
Dressing and preparation method thereof.
Background technology
Zeins (zein) is a kind of natural polymer polymer, is the topmost storage protein of corn, easily obtains
, it is economic and environment-friendly.Zeins has good biocompatibility, biodegradability etc., is a kind of environment-friendly, green
The biomaterial of color, safety.
Substantial amounts of hydrophobic amino acid is not only there is in zeins molecule, also containing more sulfur-bearing amino
Acid, with strong hydrophobicity.In polar environment, hydrophilic radical exposes in zeins molecule, and hydrophobic grouping is embedded,
Stable micellar structure can be formed.Alcohol soluble protein can dissolve in certain solvent, therefore can prepare solution, prepare film forming or
Fibrous material, insoluble cross-linked corn alcohol-soluble protein film or fibre just can be obtained after being further crosslinked using aldehydes
Dimension.Zeins easily combines to form polyblend with other compounds, and can be built according to different demands has difference
The vector delivery system of characteristic.Therefore zeins can be as the good of the liquid conductive nanofiber for preparing controllable orientation
Good raw material.
Plant extraction liquid antiseptic is plant then evolves generation to adapt to environment, and certain plants extract solution is directed to certain
There is certain control or suppression, elimination to act on for a kind of illness or some bacteriums, virus etc..For example：Compound plant is carried
Take liquid good for the inhibition of Aeromonas hydrophila, and the method extracted and separated is, it is known that operate simple and easy to do.It is existing
Nowadays, the research for extracting antiseptic from plant is more and more, therefore, will possess bigger when selecting and using
Operating space.
Plant extraction liquid antiseptic is not likely to produce drug resistance, will not Induction of bacterial virus etc. be evolved into and be more difficult to resist and disappear
Remove, solve current puzzlement people's synthetic antibacterial agents resistance problems.In addition, relative to artificial synthesized antiseptic, plant extract
Liquid antiseptic is more naturally environmentally friendly, meets current era people for natural health, the sustainable pursuit of environmental protection.
Electrostatic spinning technique (electrospinning technology) is a kind of to prepare the new of monodimension nanometer material
Technology.Early in 1934, Formhals just applied for the experimental provision on preparing polymer fiber using high-voltage electrostatic field
Patent.Afterwards, due to low production efficiency the problems such as, causes the development of the technology more slow always.Until 90 years 20th century
Generation, the rise because of nano science and the Darrell in Akron university of the U.S. (Universityof Akron)
H.Reneker teaches seminar, the Gregory of Massachusetts science and engineering (Massachusetts Institute ofTechnology)
Under the promotion of the seminars such as C.Rutledge, electrostatic spinning technique is just rapidly developed.The letter of electrostatic spinning manufacturing equipment structure
Single, cost of spinning is low, can prepare compound, hollow or solid nanofiber of continuous organic and inorganic, organic/inorganic etc., receive and grind
The extensive concern for the person of studying carefully.In recent ten years, researchers develop coaxial electrostatic spinning technology by improving needle head structure and are used for
Doughnut/nanotube is prepared, or by improving reception device, obtains a series of different patterning Nanowire of accumulation modes
Structure is tieed up, such as：Along the ANFs of the axial proper alignment of fiber, the nanofiber being axially arranged vertically with radial direction etc..It is height-oriented and
Regularly arranged nanofiber is due to special mechanics, electrical and optical properties, receiving the very big concern of researcher.
Electrostatic spinning can realize that template Shape design general principle is the point discharge using charge concentration and supporting body
Effect, wherein formwork structure are designed, and material obtains effect to specific morphology direct influence.The shape of template is received by changing
Shape, material character and the motion state for receiving template, can obtain the nanofiber felted material of various accumulation shapes.
The content of the invention
Based on above prior art, primary and foremost purpose of the invention is to provide a kind of antibacterial corn alcohol with controllable orientation
The preparation method of molten albumen dressing.
Another object of the present invention is to provide a kind of antibacterial with controllable orientation prepared by the above method
Zeins dressing.
The object of the invention is achieved through the following technical solutions：
A kind of preparation method of the antibacterial zeins dressing with controllable orientation, including following preparation process：
(1) zeins is dissolved in solvent, obtains uniform zein solution, then added antibacterial and live
Property composition, is uniformly mixed rear standing and defoaming, obtains the zein solution containing antimicrobial component；
(2) by the zein solution containing antimicrobial component by electrospinning process, respectively with rule row
Fiber dressing layer is made as receiver in the net template of the geometric figure of row, has regularly arranged striped bulge-structure with surface
Roller receiver receive obtain fiber guide layer；By fiber dressing layer and fiber guide layer superimposion, corn alcohol is obtained molten
Azelon film functional layer；
(3) zeins tunica fibrosa functional layer is combined with spun laced fabric supporting layer and polypropylene film barrier layer, by
Fiber dressing layer, fiber guide layer, spun laced fabric supporting layer and polyethylene film barrier layer are up to followed successively by down, are obtained with controllable
The antibacterial zeins dressing of orientation.
Preferably, the solvent described in step (1) refers to that ethanol or glacial acetic acid that mass concentration is 40%~98% are water-soluble
Liquid.
Preferably, described in step (1) in zein solution the mass concentration of zeins for 5%~
40%.
Preferably, the Antibacterial Constituents include but is not limited to the antibacterial activity based on microorganism or plant extract into
Point；Polylysine, Paeonol, Tea Polyphenols, eucalyptus oil, Rosa Damascana, aloe extract, honeysuckle is included but are not limited to extract
The natural antibacterial extracts such as thing, baicalin, ginkgo antibacterial protein, lemon grass (Cymbopogon citratus) essential oil, water chestnut bark extract.
Preferably, the addition of the Antibacterial Constituents is the 0.01%~5% of zeins quality.
Preferably, the net template with regularly arranged geometric figure is hexagon, rectangle, rhombus, square
Or circular net template, sizing grid is 0.1~3mm, and density is 2~40/cm2, thickness is 0.1~2.0mm.
Preferably, there is the roller receiver of regularly arranged striped bulge-structure to be a diameter of 8~50cm on the surface, long
Spend the cylindrical roller for 10~90cm；The width of fringe d of cylinder surface striped bulge-structure is 0.3~1cm, and density is 1~5
Individual/cm (i.e. adjacent stripes bulge-structure at intervals of 0.2~1cm), rising height h are that 0.2~1cm, protrusion angle α are 100
~150 °.Its structural representation is as shown in Figure 1.
Preferably, the material of the fiber dressing layer receiver includes plastics, ceramics, metal or alloy；The roller connects
Receiving the material of device includes metal or alloy.
Preferably, the method for the electrostatic spinning includes syringe needle electrostatic spinning, slit electrostatic spinning, needle-less Free Surface
Line electrode electrostatic spinning, needle-less Free Surface roller electrode electrostatic spinning, needle-less Free Surface shuttle electrode electrostatic spinning, needleless
Head Free Surface spiral line electrode electrostatic spinning or centrifugation electrostatic spinning etc..
Preferably, fibre diameter is 50~2000nm in the zeins tunica fibrosa functional layer.
A kind of antibacterial zeins dressing with controllable orientation, is prepared by the above method.
The principle of the invention is：There is the net template of geometric figure of regular arrangement using surface as receiver, figure
Arrangement have impact on the spatial arrangement of electrostatic field, grid hollow sectors electric field can weaken, and fiber deposition is less, so that tunica fibrosa
Structure it is similar to the figure of template, be covered with substantial amounts of microcellular structure, increase the gas permeability of dressing；Using surface there is rule to arrange
The roller receiver of row striped bulge-structure prepares fiber guide layer, and the roller of rotation pulls fiber so that fiber in circumference or
The raised structures that person is upwardly formed on orientation texture, roller along polygon corner angle side cause zeins tunica fibrosa to obtain
The fiber groove of complementary structure, zeins Hydrophilic Fiber is poor, and liquid is diffused into after guide layer, along fiber-wall-element model
Direction is flowed, and collects in fiber groove, and this causes sample to have certain interim liquid storage capacity, while the liquid collected is recessed
Flowed in groove to external diffusion, prevent liquid to be back to again in wound, keep wound dry and comfortable.
The preparation method and resulting dressing of the present invention has the following advantages that and beneficial effect：
(1) the antibacterial zeins dressing with controllable orientation of the invention adds natural antibacterial active component, its
Good anti-bacterial effect and have no toxic side effect, more than 99% is reached to the bacteriostasis rate of gram-positive bacteria and Gram-negative bacteria.
(2) the antibacterial zeins dressing with controllable orientation of the invention using have good biocompatibility,
The zeins of biodegradability is material of main part, environmentally friendly and economic and environment-friendly.
(3) present invention obtains composite membrane using electrostatic spinning or centrifugal spinning technology, and gained composite membrane has high porosity
And bigger serface, and its fibre structure has good similitude with extracellular matrix, available for skin injury or operation
Skin afterwards recovers the dressing with regeneration.
(4) microcellular structure has well to air and vapor in the fiber dressing layer that preparation method of the invention is obtained
Permeability or gas permeability, but there is higher resistance to hydrostatic pressure to the infiltration of aqueous water, i.e., this kind ventilating structure includes many energy
From a surface to the opening or passage on another surface, its hole is sized so that air and water vapour molecule by film, and
There is good resistance to liquid water molecules；And because the addition of one or more antiseptics causes with good antibacterial work
Property, antiseptic can be released under certain condition, may be used as dressing materials.
(5) dressing of the invention is from fiber dressing layer to spun laced fabric supporting layer, and the moisture pick-up properties of dressing gradually strengthens, and is formed
Good leads wet gradient.In the presence of differential capillary effect, directional water guiding is realized.With the guide layer dressing energy being well orientated
It is enough that a stable balance is reached between infiltration and diffusion.When wound fluid or wound purulence thing are arrived through tunica fibrosa functional layer
During up to guide layer, because guide layer has certain thickness fluff structure and the distribution of larger longitudinal fiber, thus diffusate or
Purulence thing can quickly be captured and along longitudinal diffusion, so that liquid is slow and effectively enters guide layer, and temporarily will
Fluid preservation, it is to avoid liquid keeps wound dry and comfortable not by bleeding back for absorbing and cause in time.
Brief description of the drawings
Fig. 1 is the structural representation of cylinder surface striped bulge-structure of the present invention；
Fig. 2 is the stepped construction schematic diagram of dressing obtained by the embodiment of the present invention；
Fig. 3 is the structural representation of fiber dressing layer and fiber guide layer in the gained dressing of the embodiment of the present invention 1；
Fig. 4 is the structural representation of fiber dressing layer and fiber guide layer in the gained dressing of the embodiment of the present invention 2；
Fig. 5 is the structural representation of fiber dressing layer and fiber guide layer in the gained dressing of the embodiment of the present invention 3；
Numbering is described as follows in figure：1- polypropylene film barrier layers, 2- spun laced fabric supporting layers, 3- fiber guide layers, 4- is fine
Tie up dressing layer.
Embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited
In this.
Embodiment 1
Take the last particle of the pure zein powder of analysis, from mass concentration for 40% ethanol solution as solvent, often
Temperature stirring 30min, obtains mass concentration for 5% uniform zein solution；Treat that zein solution is cooled to
After room temperature, then weigh in a certain amount of aloe antibiotic extract addition zein solution, stirring at normal temperature 1h obtains aloe
Extract quality percentage composition is 0.1% zeins-plants antimicrobial extract solution, standing and defoaming 1h.It will prepare
Mixed solution zeins-plant extract fibres guide layer is prepared using Free Surface line electrode electrospinning process
And fiber dressing layer.Under 65kV voltage, zeins-plants antimicrobial extract solution is in the rotating speed of line electrode
Carry out electrospinning under the conditions of 10r/min, and away from being received at syringe needle about 10cm, be that 90cm, rotating speed are from a diameter of 50cm, length
1200r/min's, surface have width be 1cm, density be 5/cm, be highly 1cm, the straight line metal that protrusion angle is 120 °
Raised roller receiver, which is received, can obtain fiber guide layer；From surface there is size to be that 0.5mm, density are 40/cm2, thickness
Fiber dressing layer can be obtained by being received for 0.1mm roundness mess templates receiver, the structure of gained fiber dressing layer and fiber guide layer
Schematic diagram is as shown in Figure 3.By fiber dressing layer and fiber guide layer superimposion, zeins tunica fibrosa function is obtained
Layer.Fibre diameter is 900~1200nm in gained zeins tunica fibrosa functional layer.By zeins tunica fibrosa work(
Ergosphere and spun laced fabric supporting layer and polypropylene film barrier layer are compound, be from top to bottom followed successively by fiber dressing layer, fiber guide layer,
Spun laced fabric supporting layer and polypropylene film barrier layer, obtain the antibacterial zeins dressing with controllable orientation.Gained is applied
The stepped construction schematic diagram of material is as shown in Figure 2.
The antibiotic rate test of antibacterial zeins dressing obtained by the present embodiment：
Take (10 ± 0.1) ml agar in each sterile petri dish, allow agar solidified.Take requirement agar, heating water bath to (45
±1)℃.It is (1-5 × 10 by bacterial concentration8Cfu/ml microbionation) makes bacterium on 150ml agar with forced oscillation container
It is dispersed.(5 ± 0.1) ml is on culture dish, allows agar solidification.Using the nutrition fine jade in inoculation time one hour during experiment
Fat culture dish.According to EN ISO 20645:2004 methods are operated.Pressed with sterilizing tweezers in the middle of culture and pave certain area
Antibacterial zeins dressing, fiber dressing layer and bacterium is had good contact, at (37 ± 1) DEG C after culture 18h to 24h
Count plate.According to EN ISO 20645:2004 methods determine viable count, obtain the present embodiment antibacterial zeins dressing
Antibiotic rate be 99.67%.
Embodiment 2
Take the last particle of the pure zein powder of analysis, from mass concentration for 65% ethanol solution as solvent, often
Temperature stirring 30min, obtains mass concentration for 18% uniform zein solution；Treat that zein solution is cooled to
After room temperature, then weigh in a certain amount of Paeonol addition zein solution, stirring at normal temperature 1h obtains Paeonol quality hundred
Divide zeins-plants antimicrobial extract solution that content is 0.4%, standing and defoaming 1h.The mixed solution prepared is adopted
Zeins-plant extract fibres guide layer and fiber dressing layer are prepared with syringe needle electrospinning process.9kV's
Under voltage, zeins-plants antimicrobial extract solution carries out electrospinning with the flow velocity in 0.4ml/h/ holes, and away from syringe needle about
Received at 10cm, be that 60cm, rotating speed are 1300r/min from a diameter of 9cm, length, surface has width for 0.5cm, close
Spend for 5/cm, be highly 0.2cm, the roller receiver for the straight line metal bump that protrusion angle is 120 ° receives and can obtain fiber and lead
Fluid layer；From surface there is size to be that 1mm, density are 30/cm2, thickness be 0.5mm square net templates receiver receive
Fiber dressing layer can be obtained, the structural representation of gained fiber dressing layer and fiber guide layer is as shown in Figure 4.By fiber dressing layer with
Fiber guide layer superimposion, obtains zeins tunica fibrosa functional layer.Gained zeins tunica fibrosa functional layer
Middle fibre diameter is about 800~1500nm.Zeins tunica fibrosa functional layer and spun laced fabric supporting layer and polypropylene is thin
Film barrier layer is combined, and is from top to bottom followed successively by fiber dressing layer, fiber guide layer, spun laced fabric supporting layer and polypropylene film barrier
Layer, obtains the antibacterial zeins dressing with controllable orientation.The stepped construction schematic diagram of gained dressing is as shown in Figure 2.
Antibiotic rate is 99.95% after tested for antibacterial zeins dressing obtained by the present embodiment.
Embodiment 3
The last particle of the pure zein powder of analysis is taken, from the glacial acetic acid solution that mass concentration is 90% as solvent,
Stirring at normal temperature 30min, obtains mass concentration for 19% uniform zein solution；Treat that zein solution is cooled down
To after room temperature, then weigh in a certain amount of eucalyptus oil addition zein solution, stirring at normal temperature 1h obtains eucalyptus oil quality
Percentage composition is 0.01% zeins-plants antimicrobial extract solution, standing and defoaming 1h.By the mixed solution prepared
Using needle-less Free Surface roller electrode electrospinning process prepare zeins-plant extract fibres guide layer and
Fiber dressing layer.Under 70kV voltage, zeins-plants antimicrobial extract solution is carried out with 0.4ml/h flow velocity
Electrospinning, and be that 10cm, rotating speed are 1500r/min, surface from a diameter of 40cm, length away from being received at syringe needle about 10cm
With width be 0.3cm, density be 1/cm, be highly 0.5cm, the roller for the straight line metal bump that protrusion angle is 150 ° connects
Fiber guide layer can be obtained by receiving device reception；From surface there is size to be that 0.5mm, density are 30/cm2, thickness be 10mm positive six
Side shape net template receiver, which is received, can obtain fiber dressing layer, and the structural representation of gained fiber dressing layer and fiber guide layer is such as
Shown in Fig. 5.By fiber dressing layer and fiber guide layer superimposion, zeins tunica fibrosa functional layer is obtained.Gained is beautiful
Fibre diameter is 1200~2000nm in rice alcohol soluble protein tunica fibrosa functional layer.By zeins tunica fibrosa functional layer and water
Thorn cloth supporting layer and polypropylene film barrier layer are compound, are from top to bottom followed successively by fiber dressing layer, fiber guide layer, spun laced fabric branch
Layer and polypropylene film barrier layer are supportted, the antibacterial zeins dressing with controllable orientation is obtained.The stacking of gained dressing
Structural representation is as shown in Figure 2.
Antibiotic rate is 99.69% after tested for antibacterial zeins dressing obtained by the present embodiment.
Embodiment 4
The last particle of the pure zein powder of analysis is taken, from the glacial acetic acid solution that mass concentration is 98% as solvent,
Stirring at normal temperature 30min, obtains mass concentration for 20% uniform zein solution；Treat that zein solution is cooled down
To after room temperature, then weigh in a certain amount of Tea Polyphenols addition zein solution, stirring at normal temperature 1h obtains Tea Polyphenols quality
Percentage composition is 5% zeins-plants antimicrobial extract solution, standing and defoaming 1h.The mixed solution prepared is adopted
Prepared with needle-less Free Surface screw electrode electrospinning process zeins-plant extract fibres guide layer and
Fiber dressing layer.Under 60kV voltage, zeins-plants antimicrobial extract solution is carried out with 1.2ml/h flow velocity
Electrospinning, and be that 60cm, rotating speed are 1600r/min, surface from a diameter of 13cm, length away from being received at syringe needle about 10cm
With width be 1cm, density be 2/cm, be highly 1cm, the roller receiver for the straight line metal bump that protrusion angle is 100 °
Reception can obtain fiber guide layer；From surface there is size to be that 1mm, density are 40/cm2, thickness be 5mm roundness mess templates
Receiver, which is received, can obtain fiber dressing layer, by fiber dressing layer and fiber guide layer superimposion, obtain zeins fine
Tie up film functional layer.Fibre diameter is 700~1300nm in gained zeins tunica fibrosa functional layer.By zeins
Tunica fibrosa functional layer and spun laced fabric supporting layer and polypropylene film barrier layer are compound, are from top to bottom followed successively by fiber dressing layer, fibre
Guide layer, spun laced fabric supporting layer and polypropylene film barrier layer are tieed up, the antibacterial zeins with controllable orientation is obtained and applies
Material.The stepped construction schematic diagram of gained dressing is as shown in Figure 2.
Antibiotic rate is 99.99% after tested for antibacterial zeins dressing obtained by the present embodiment.
Embodiment 5
Take the last particle of the pure zein powder of analysis, from mass concentration for 98% ethanol solution as solvent, often
Temperature stirring 30min, obtains mass concentration for 40% uniform zein solution；Treat that zein solution is cooled to
After room temperature, then weigh in a certain amount of polylysine addition zein solution, stirring at normal temperature 1h obtains polylysine matter
Measure zeins-plants antimicrobial extract solution that percentage composition is 3%, standing and defoaming 1h.By the mixed solution prepared
Zeins-plant extract fibres guide layer and fiber dressing layer are prepared using centrifugation electrospinning process.
Under 35kV voltage, zeins-plants antimicrobial extract solution carries out electrospinning with 2.5ml/h flow velocity, centrifuges electrostatic
Spinning centrifugation disc spin speed be 300r/min, and away from syringe needle about 10cm at receive, from a diameter of 8cm, length be 50cm,
Rotating speed for 1500r/min, surface have width be 0.8cm, density be 1/cm, be highly 1cm, protrusion angle is 110 °
The roller receiver of straight line metal bump, which is received, can obtain fiber guide layer；From surface have size be 3mm, density be 2/
cm2, thickness be that 20mm roundness mess templates receiver receives and can obtain fiber dressing layer, fiber dressing layer and fiber water conservancy diversion are laminated
Plus it is compound, obtain zeins tunica fibrosa functional layer.Fibre diameter is in gained zeins tunica fibrosa functional layer
1000~1500nm.Zeins tunica fibrosa functional layer is combined with spun laced fabric supporting layer and polypropylene film barrier layer,
From top to bottom be followed successively by fiber dressing layer, fiber guide layer, spun laced fabric supporting layer and polypropylene film barrier layer, obtain have can
Control the antibacterial zeins dressing of orientation.The stepped construction schematic diagram of gained dressing is as shown in Figure 2.
Antibiotic rate is 99.98% after tested for antibacterial zeins dressing obtained by the present embodiment.
Embodiment 6
The last particle of the pure zein powder of analysis is taken, from the glacial acetic acid solution that mass concentration is 90% as solvent,
Stirring at normal temperature 30min, obtains mass concentration for 25% uniform zein solution；Treat that zein solution is cooled down
To after room temperature, then weigh in a certain amount of aloe antibiotic extract addition zein solution, stirring at normal temperature 1h obtains reed
Luxuriant growth antimicrobial extract weight/mass percentage composition is 2% zeins-plants antimicrobial extract solution, standing and defoaming 1h.Will
The mixed solution prepared prepares zeins-plant extracts using needle-less Free Surface shuttle electrode electrospinning process
Fiber guide layer and fiber dressing layer.Under 10kV voltage, zeins-plants antimicrobial extract solution with
3.0ml/h flow velocity carries out electrospinning, and away from being received at syringe needle about 10cm, is from a diameter of 12cm, a diameter of 80cm, rotating speed
1400r/min's, surface have width be 0.3cm, density be 2/cm, be highly 0.4cm, the straight line that protrusion angle is 125 °
The roller receiver of metal bump, which is received, can obtain fiber guide layer；From surface there is size to be that 2mm, density are 20/cm2、
Thickness is that the reception of 8mm network templates receiver can obtain fiber dressing layer, fiber dressing layer is superimposed with fiber guide layer multiple
Close, obtain zeins tunica fibrosa functional layer.Fibre diameter is 1000 in gained zeins tunica fibrosa functional layer
~1200nm.Zeins tunica fibrosa functional layer is combined with spun laced fabric supporting layer and polypropylene film barrier layer, by upper
Fiber dressing layer, fiber guide layer, spun laced fabric supporting layer and polypropylene film barrier layer are followed successively by under, obtains taking with controllable
To antibacterial zeins dressing.The stepped construction schematic diagram of gained dressing is as shown in Figure 2.
Antibiotic rate is 99.93% after tested for antibacterial zeins dressing obtained by the present embodiment.
Embodiment 7
Take the last particle of the pure zein powder of analysis, from mass concentration for 95% ethanol solution as solvent, often
Temperature stirring 30min, obtains mass concentration for 25% uniform zein solution；Treat that zein solution is cooled to
After room temperature, then weigh a certain amount of aloe and by the oily (mass ratio 1 of tree:2) composite antibacterial extract addition zeins is molten
In liquid, stirring at normal temperature 1h obtains zeins-plants antimicrobial that composite antibacterial extract quality percentage composition is 1.0%
Extract solution, standing and defoaming 1h.The mixed solution prepared is prepared into zeins-plant using syringe needle electrospinning process
Thing extracts fibres guide layer and fiber dressing layer.Under 13kV voltage, zeins-plants antimicrobial extract is molten
Liquid carries out electrospinning with the flow velocity in 0.4ml/h/ holes, and away from being received at syringe needle about 10cm, is from a diameter of 20cm, length
60cm, rotating speed for 1800r/min, surface have width be 1cm, density be 1/cm, be highly 0.5cm, protrusion angle is
The roller receiver of 135 ° of straight line metal bump, which is received, can obtain fiber guide layer；From surface there is size to be that 1mm, density are
30/cm2, thickness be 0.5mm square net templates receiver receive can obtain fiber dressing layer, by fiber dressing layer and fiber
Guide layer superimposion, obtains zeins tunica fibrosa functional layer.It is fine in gained zeins tunica fibrosa functional layer
Tie up a diameter of 50~1000nm.By zeins tunica fibrosa functional layer and spun laced fabric supporting layer and polypropylene film barrier layer
It is compound, fiber dressing layer, fiber guide layer, spun laced fabric supporting layer and polypropylene film barrier layer are from top to bottom followed successively by, is obtained
Antibacterial zeins dressing with controllable orientation.The stepped construction schematic diagram of gained dressing is as shown in Figure 2.
Antibiotic rate is 99.99% after tested for antibacterial zeins dressing obtained by the present embodiment.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. a kind of preparation method of the antibacterial zeins dressing with controllable orientation, it is characterised in that including following preparation
Step：
(1) zeins is dissolved in solvent, obtains uniform zein solution, then add antibacterial activity into
Point, rear standing and defoaming is uniformly mixed, the zein solution containing antimicrobial component is obtained；
(2) by the zein solution containing antimicrobial component by electrospinning process, respectively with regularly arranged
Fiber dressing layer is made as receiver in the net template of geometric figure, has the rolling of regularly arranged striped bulge-structure with surface
Cylinder receiver, which is received, obtains fiber guide layer；By fiber dressing layer and fiber guide layer superimposion, zeins is obtained
Tunica fibrosa functional layer；
(3) zeins tunica fibrosa functional layer is combined with spun laced fabric supporting layer and polypropylene film barrier layer, by up to
Under be followed successively by fiber dressing layer, fiber guide layer, spun laced fabric supporting layer and polyethylene film barrier layer, obtain with controllable orientation
Antibacterial zeins dressing.
2. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its
It is characterised by：Solvent described in step (1) refers to the ethanol or glacial acetic acid aqueous solution that mass concentration is 40%~98%.
3. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its
It is characterised by：The mass concentration of zeins is 5%~40% in zein solution described in step (1).
4. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its
It is characterised by：The Antibacterial Constituents include polylysine, Paeonol, Tea Polyphenols, eucalyptus oil, Rosa Damascana, aloe and extracted
Thing, Honegsukle flower P.E, baicalin, ginkgo antibacterial protein, lemon grass (Cymbopogon citratus) essential oil or water chestnut bark extract.
5. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its
It is characterised by：The addition of the Antibacterial Constituents is the 0.1%~5% of zein solution quality.
6. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its
It is characterised by：The net template with regularly arranged geometric figure is the net template of hexagon, square or circle,
Sizing grid is 0.1~3mm, and density is 2~40/cm2, thickness is 0.1~2.0mm.
7. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its
It is characterised by：There is the roller receiver of regularly arranged striped bulge-structure to be a diameter of 8~15cm on the surface, and length is 15
~30cm cylindrical roller；The width of fringe of the striped bulge-structure of cylinder surface be 0.3~1cm, density be 1~5/cm,
Rising height is that 0.2~1cm, protrusion angle are 100~150 °.
8. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its
It is characterised by：The method of the electrostatic spinning includes single needle electrostatic spinning, slit electrostatic spinning, needle-less Free Surface upper thread electricity
Pole electrostatic spinning, needle-less Free Surface roller electrode electrostatic spinning, needle-less Free Surface shuttle electrode electrostatic spinning, needle-less are certainly
By perficial helical line electrode electrostatic spinning or centrifugation electrostatic spinning.
9. a kind of preparation method of antibacterial zeins dressing with controllable orientation according to claim 1, its
It is characterised by：Fibre diameter is 50~2000nm in the zeins tunica fibrosa functional layer.
10. a kind of antibacterial zeins dressing with controllable orientation, it is characterised in that：It is any by claim 1~9
Method described in is prepared.
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| CN110306290A (en) * | 2019-07-29 | 2019-10-08 | 广东工业大学 | A kind of preparation method of the anti-oxidant loaded nano tunica fibrosa of edible antibacterial |
| CN111850837A (en) * | 2020-07-24 | 2020-10-30 | 吉林农业大学 | Zein-based uniaxial electrostatic spinning oriented fiber film and preparation method thereof |
| CN112376124A (en) * | 2020-11-20 | 2021-02-19 | 上海市第六人民医院 | Antibacterial dressing |
| CN112647193A (en) * | 2020-12-31 | 2021-04-13 | 上海市第六人民医院 | Preparation method of electron beam irradiation crosslinked calcium peroxide-carbon quantum dot @ zein antibacterial film |
| CN112587710A (en) * | 2021-02-05 | 2021-04-02 | 甘肃省分析测试中心 | Preparation process and application of peach kernel extract core-shell nano-structure film |
| CN113417074A (en) * | 2021-05-24 | 2021-09-21 | 江苏省农业科学院 | Preparation method of antibacterial nanofiber membrane |
| CN114752091A (en) * | 2022-03-25 | 2022-07-15 | 中国科学院兰州化学物理研究所 | Preparation method of zein/honeysuckle extract composite antibacterial preservative film |
| CN115531595A (en) * | 2022-09-28 | 2022-12-30 | 长春工业大学 | Antibacterial healing-promoting electrostatic spinning wound dressing and preparation method thereof |
| CN115531595B (en) * | 2022-09-28 | 2023-08-15 | 长春工业大学 | Antibacterial healing-promoting electrostatic spinning wound dressing and preparation method thereof |
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