CN115531595A - Antibacterial healing-promoting electrostatic spinning wound dressing and preparation method thereof - Google Patents
Antibacterial healing-promoting electrostatic spinning wound dressing and preparation method thereof Download PDFInfo
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- CN115531595A CN115531595A CN202211188074.3A CN202211188074A CN115531595A CN 115531595 A CN115531595 A CN 115531595A CN 202211188074 A CN202211188074 A CN 202211188074A CN 115531595 A CN115531595 A CN 115531595A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 39
- 238000010041 electrostatic spinning Methods 0.000 title claims abstract description 22
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 21
- 229920001661 Chitosan Polymers 0.000 claims abstract description 11
- 229920002494 Zein Polymers 0.000 claims abstract description 9
- 239000005019 zein Substances 0.000 claims abstract description 9
- 229940093612 zein Drugs 0.000 claims abstract description 9
- 229920002037 poly(vinyl butyral) polymer Polymers 0.000 claims abstract description 7
- 239000002243 precursor Substances 0.000 claims description 48
- 239000007788 liquid Substances 0.000 claims description 38
- 239000000243 solution Substances 0.000 claims description 31
- 238000009987 spinning Methods 0.000 claims description 17
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 claims description 12
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 9
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 229920000128 polypyrrole Polymers 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 238000001132 ultrasonic dispersion Methods 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 4
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 claims 2
- 229910001870 ammonium persulfate Inorganic materials 0.000 claims 1
- 230000000845 anti-microbial effect Effects 0.000 claims 1
- 239000007800 oxidant agent Substances 0.000 claims 1
- 230000001590 oxidative effect Effects 0.000 claims 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 claims 1
- 206010052428 Wound Diseases 0.000 abstract description 32
- 208000027418 Wounds and injury Diseases 0.000 abstract description 32
- 239000000835 fiber Substances 0.000 abstract description 5
- 239000012528 membrane Substances 0.000 abstract description 5
- 230000029663 wound healing Effects 0.000 abstract description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- PQLVXDKIJBQVDF-UHFFFAOYSA-N acetic acid;hydrate Chemical compound O.CC(O)=O PQLVXDKIJBQVDF-UHFFFAOYSA-N 0.000 description 4
- 230000004888 barrier function Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000011258 core-shell material Substances 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002121 nanofiber Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/09—Addition of substances to the spinning solution or to the melt for making electroconductive or anti-static filaments
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
- D01F1/103—Agents inhibiting growth of microorganisms
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F4/00—Monocomponent artificial filaments or the like of proteins; Manufacture thereof
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4382—Stretched reticular film fibres; Composite fibres; Mixed fibres; Ultrafine fibres; Fibres for artificial leather
- D04H1/43825—Composite fibres
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06B—TREATING TEXTILE MATERIALS USING LIQUIDS, GASES OR VAPOURS
- D06B15/00—Removing liquids, gases or vapours from textile materials in association with treatment of the materials by liquids, gases or vapours
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Textile Engineering (AREA)
- Public Health (AREA)
- Hematology (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Manufacturing & Machinery (AREA)
- Dispersion Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses an antibacterial healing-promoting electrostatic spinning wound dressing and a preparation method thereof, and belongs to the field of dressing application materials. The invention adopts the parallel double-nozzle electrostatic spinning technology, the preparation method is superior to the wound dressing prepared by single-nozzle electrostatic spinning, and the invention simultaneously performs electrostatic spinning on two immiscible solutions of zein, polyvinyl butyral solution, chitosan and ethylene oxide solution to obtain two fiber membranes with uniformly distributed fibers, so that the components in the fiber membranes can provide services for wound healing more effectively.
Description
Technical Field
The invention belongs to the technical field of biomedical dressings, and particularly relates to an antibacterial healing-promoting electrostatic spinning wound dressing and a preparation method thereof.
Background
The skin is the largest organ separating the internal environment from the external environment of the human body, and can be inevitably injured by the external world to generate wounds, so the wound dressing is produced by transportation. In general, wounds are exposed to the outside, and therefore, the wound dressing needs to satisfy requirements such as antibacterial property, barrier, liquid absorption, easy gas exchange, and wound healing promotion, and portability is also being considered.
Chinese patent CN103550816A discloses a preparation method of a chitosan-based electrostatic spinning composite wound dressing, which is characterized in that chitosan is coated on a receiver to perform electrostatic spinning, and compared with complex operation, the parallel spinning technology can prepare the wound dressing which is uniformly mixed in one step.
Chinese patent CN107158447A discloses an antibacterial zein dressing with controllable orientation and a preparation method thereof, which is characterized in that a template method is used for preparing an electrostatic spinning wound dressing with a multilayer structure, and due to the characteristic of gas permeation of the multilayer structure, gas through holes are influenced, so that the gas exchange efficiency is influenced.
Chinese patent CN114225090A discloses a chitosan-based nanofiber wound dressing with adhesion and antibacterial performance and a preparation method thereof. The wound dressing is characterized in that a core-shell structure is adopted to prepare the wound dressing, and the fiber membrane is easy to tear or cause secondary damage to the wound during movement due to weak strength of the fiber membrane.
Compared with the membrane prepared in the patent, the preparation is complicated; the structure is complex; the double-needle parallel spinning technology can simultaneously carry out electrostatic spinning on two spinning solutions at the same or different propelling speeds, so that an electrostatic spinning film with uniform component dispersion, multiple functions, simple overall structure, looseness, porosity and certain strength is obtained.
Disclosure of Invention
The invention provides an antibacterial healing-promoting electrostatic spinning wound dressing and a preparation method thereof, aiming at solving the problems of complicated process, thick dressing and insufficient strength in the wound dressing.
The invention is characterized in that the active substances of the wound dressing are uniformly mixed by adopting the parallel double-needle electrostatic spinning technology, and the wound dressing is thin in film and strong in strength.
The invention provides an antibacterial healing-promoting electrostatic spinning wound dressing and a preparation method thereof, and the steps and conditions are as follows:
(1) Preparation of polyazoles
Dissolving pyrrole in an ethanol water solution at a ratio of 1;
(2) Preparation of precursor liquid A
Dissolving zein with the mass concentration of 5-35% and polyvinyl butyral with the mass concentration of 1~8% in an ethanol water solution, adding the purified polypyrrole prepared in the step into the solution with the mass concentration of 1-10%, and performing ultrasonic dispersion for 1 hour to uniformly mix to obtain a precursor solution A;
(3) Preparation of precursor liquid B
Dissolving chitosan with the mass concentration of 1-4% and polyethylene oxide with the mass concentration of 1~3% in 1-3% (v/v) acetic acid aqueous solution, and fully stirring and uniformly dissolving to obtain precursor solution B;
(4) Preparation of electrospun wound dressings
Respectively extracting the precursor liquid A and the precursor liquid B by using an injector, connecting a parallel needle, and under the voltage of 10 to 25kV and under the condition of taking 9 to 20cm as a collecting distance, obtaining a precursor liquid A:0.2 to 1ml/h; precursor liquid B: simultaneously pushing an injector for spinning at 0.1-0.3 ml/h, and collecting spinning products on a collector;
(5) Post-treatment
And (4) washing the spinning product collected in the step (4) by using deionized water, and drying the washed product in a vacuum oven for 12 hours to obtain the antibacterial healing-promoting electrostatic spinning wound dressing.
The invention has the beneficial effects that: the loose and porous structure can be obtained by adopting the preparation method; has broad-spectrum antibacterial ability; and the zein can be used as a temporary barrier to isolate the surrounding environment of the wound and resist the invasion of microorganisms in the air to the wound together with chitosan.
Detailed Description
The present invention will be described in further detail with reference to examples. However, the method of carrying out the present invention is not limited thereto.
Example 1
(1) Preparation of polyazoles
Dissolving pyrrole in an ethanol water solution at a ratio of 1;
(2) Preparation of precursor liquid A
Dissolving zein with the mass concentration of 5% and polyvinyl butyral with the mass concentration of 1% in an ethanol water solution, adding the purified polypyrrole prepared in the step into the solution with the mass concentration of 1%, and performing ultrasonic dispersion for 1 hour to uniformly mix to obtain a precursor solution A;
(3) Preparation of precursor liquid B
Dissolving chitosan with the mass concentration of 1% and polyethylene oxide with the mass concentration of 1% in 1% (v/v) acetic acid water solution, fully stirring and uniformly dissolving to obtain precursor liquid B;
(4) Preparation of electrospun wound dressings
Respectively extracting the precursor liquid A and the precursor liquid B by using an injector, connecting parallel needles, and under the voltage of 10kV and on the premise of taking 9cm as a collecting distance, obtaining the precursor liquid A:0.2ml/h; precursor liquid B:0.1ml/h simultaneously pushes an injector to spin, and a spinning product is collected on a collector;
(5) Post-treatment
And (4) washing the spinning product collected in the step (4) by using deionized water, and drying the washed product in a vacuum oven for 12 hours to obtain the antibacterial healing-promoting electrostatic spinning wound dressing.
Example 2
(1) Preparation of polyazoles
Dissolving pyrrole in an ethanol aqueous solution at a ratio of 1;
(2) Preparation of precursor solution A
Dissolving 35% by mass of zein and 8% by mass of polyvinyl butyral into an ethanol aqueous solution, adding the purified polypyrrole prepared in the step into the solution at the mass concentration of 10%, and performing ultrasonic dispersion for 1 hour to uniformly mix to obtain a precursor solution A;
(3) Preparation of precursor liquid B
Dissolving chitosan with the mass concentration of 4% and polyethylene oxide with the mass concentration of 3% in 3% (v/v) acetic acid water solution, fully stirring and uniformly dissolving to obtain precursor liquid B;
(4) Preparation of electrospun wound dressings
Respectively extracting the precursor liquid A and the precursor liquid B by using an injector, connecting parallel needles, and under the voltage of 25kV and on the premise of taking 20cm as a collecting distance, obtaining a precursor liquid A:1ml/h; precursor liquid B:0.3ml/h simultaneously pushes an injector to carry out spinning, and a spinning product is collected on a collector;
(5) Post-treatment
And (4) washing the spinning product collected in the step (4) by using deionized water, and drying the washed product in a vacuum oven for 12 hours to obtain the antibacterial healing-promoting electrostatic spinning wound dressing.
Example 3
(1) Preparation of polyazoles
Dissolving pyrrole in an ethanol water solution at a ratio of 1;
(2) Preparation of precursor liquid A
Dissolving 35% by mass of zein and 8% by mass of polyvinyl butyral into an ethanol aqueous solution, adding the purified polypyrrole prepared in the step into the solution at the mass concentration of 10%, and performing ultrasonic dispersion for 1 hour to uniformly mix to obtain a precursor solution A;
(3) Preparation of precursor liquid B
Dissolving chitosan with the mass concentration of 4% and polyethylene oxide with the mass concentration of 3% in 3% (v/v) acetic acid water solution, fully stirring and uniformly dissolving to obtain precursor liquid B;
(4) Preparation of electrospun wound dressings
Respectively extracting the precursor liquid A and the precursor liquid B by using an injector, connecting parallel needles, and under the voltage of 25kV and on the premise of taking 20cm as a collecting distance, obtaining a precursor liquid A:1ml/h; precursor liquid B:0.3ml/h simultaneously pushes an injector to spin, and a spinning product is collected on a collector;
(5) Post-treatment
And (4) washing the spinning product collected in the step (4) by using deionized water, and drying the washed product in a vacuum oven for 12 hours to obtain the antibacterial healing-promoting electrostatic spinning wound dressing.
Example 4
(1) Preparation of polyazoles
Dissolving pyrrole in an ethanol water solution at a ratio of 1;
(2) Preparation of precursor liquid A
Dissolving zein with the mass concentration of 5% and polyvinyl butyral with the mass concentration of 1% in an ethanol water solution, adding the purified polypyrrole prepared in the step into the solution with the mass concentration of 1%, and performing ultrasonic dispersion for 1 hour to uniformly mix to obtain a precursor solution A;
(3) Preparation of precursor liquid B
Dissolving chitosan with the mass concentration of 1% and polyethylene oxide with the mass concentration of 1% in 1% (v/v) acetic acid water solution, fully stirring and uniformly dissolving to obtain a precursor solution B;
(4) Preparation of electrospun wound dressings
Respectively extracting the precursor liquid A and the precursor liquid B by using an injector, connecting parallel needles, and under the voltage of 10kV and on the premise of taking 9cm as a collecting distance, obtaining the precursor liquid A:0.2ml/h; precursor liquid B:0.1ml/h simultaneously pushes an injector to spin, and a spinning product is collected on a collector;
(5) Post-treatment
And (4) washing the spinning product collected in the step (4) by using deionized water, and drying the washed product in a vacuum oven for 12 hours to obtain the antibacterial healing-promoting electrostatic spinning wound dressing.
Claims (6)
1. An antibacterial healing-promoting electrostatic spinning wound dressing is characterized by comprising the following steps and conditions:
(1) Preparation of polyazoles
Dissolving pyrrole in an ethanol water solution at a ratio of 1;
(2) Preparation of precursor solution A
Dissolving zein with the mass concentration of 5-35% and polyvinyl butyral with the mass concentration of 1~8% in an ethanol water solution, adding the purified polypyrrole prepared in the step into the solution with the mass concentration of 1-10%, and performing ultrasonic dispersion for 1 hour to uniformly mix to obtain a precursor solution A;
(3) Preparation of precursor liquid B
Dissolving chitosan with the mass concentration of 1-4% and polyethylene oxide with the mass concentration of 1~3% in 1-3% (v/v) acetic acid aqueous solution, and fully stirring and uniformly dissolving to obtain precursor solution B;
(4) Preparation of electrospun wound dressings
Respectively extracting the precursor liquid A and the precursor liquid B by using an injector, connecting a parallel needle, and under the voltage of 10 to 25kV and under the condition of taking 9 to 20cm as a collecting distance, obtaining a precursor liquid A:0.2 to 1ml/h; precursor liquid B: simultaneously propelling an injector to spin at 0.1 to 0.3ml/h, and collecting spinning products on a collector;
(5) Post-treatment
And (4) washing the spinning product collected in the step (4) by using deionized water, and drying the washed product in a vacuum oven for 12 hours to obtain the antibacterial healing-promoting electrostatic spinning wound dressing.
2. The antibacterial healing-promoting electrospun wound dressing and the preparation method thereof as claimed in claim 1, wherein the oxidant in (1) is one of ammonium persulfate and potassium persulfate.
3. The antibacterial healing-promoting electrospun wound dressing and the preparation method thereof as claimed in claim 1, wherein the molecular weight of the polyethylene oxide in the step (3) is between 60 and 400W.
4. The antibacterial healing-promoting electrospun wound dressing and the preparation method thereof as claimed in claim 1, wherein the number of the parallel needles used in the step (4) is 22 to 26G/24 to 27G.
5. An antibacterial healing-promoting electrospun wound dressing prepared according to the method of any one of claims 1 to 4.
6. Use of the antimicrobial healing-promoting electrospun wound dressing according to claim 5 in a wound dressing.
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| CN202211188074.3A CN115531595B (en) | 2022-09-28 | 2022-09-28 | Antibacterial healing-promoting electrostatic spinning wound dressing and preparation method thereof |
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| CN202211188074.3A CN115531595B (en) | 2022-09-28 | 2022-09-28 | Antibacterial healing-promoting electrostatic spinning wound dressing and preparation method thereof |
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| CN115531595B CN115531595B (en) | 2023-08-15 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012091636A2 (en) * | 2010-12-30 | 2012-07-05 | Федеральное Государственное Бюджетное Образовательное Учреждение Высшего Профессионального Образования "Саратовский Государственный Университет Имени Н.Г. Чернышевского" | Biopolymer fibre, composition of a forming solution for producing same, method for preparing a forming solution, fabric for biomedical use, method for modifying same, biological dressing and method for treating wounds |
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| CN113089185A (en) * | 2021-04-02 | 2021-07-09 | 南通大学 | Conductive nanofiber membrane with sterilization function and preparation method and application thereof |
| CN113699696A (en) * | 2021-09-06 | 2021-11-26 | 长春工业大学 | Multilayer functional wound dressing prepared by adopting portable electrospinning technology |
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2022
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| WO2012091636A2 (en) * | 2010-12-30 | 2012-07-05 | Федеральное Государственное Бюджетное Образовательное Учреждение Высшего Профессионального Образования "Саратовский Государственный Университет Имени Н.Г. Чернышевского" | Biopolymer fibre, composition of a forming solution for producing same, method for preparing a forming solution, fabric for biomedical use, method for modifying same, biological dressing and method for treating wounds |
| CN107158447A (en) * | 2017-07-06 | 2017-09-15 | 华南理工大学 | A kind of antibacterial zeins dressing with controllable orientation and preparation method thereof |
| CN107261192A (en) * | 2017-07-27 | 2017-10-20 | 江南大学 | The bitter certain kind of berries glycosides F1 dressing and preparation method of a kind of promotion wound Scarless wound healing |
| CN108714239A (en) * | 2018-05-30 | 2018-10-30 | 广东泰宝医疗器械技术研究院有限公司 | A kind of high liquid-absorbing chitosan anti-bacteria glue dressing and preparation method thereof |
| CN113089185A (en) * | 2021-04-02 | 2021-07-09 | 南通大学 | Conductive nanofiber membrane with sterilization function and preparation method and application thereof |
| CN113699696A (en) * | 2021-09-06 | 2021-11-26 | 长春工业大学 | Multilayer functional wound dressing prepared by adopting portable electrospinning technology |
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