CN110122424B - Device for inducing ladybug to lay eggs - Google Patents
Device for inducing ladybug to lay eggs Download PDFInfo
- Publication number
- CN110122424B CN110122424B CN201910403928.7A CN201910403928A CN110122424B CN 110122424 B CN110122424 B CN 110122424B CN 201910403928 A CN201910403928 A CN 201910403928A CN 110122424 B CN110122424 B CN 110122424B
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- spawning
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- coccinella
- pea
- coccinella septempunctata
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- 235000013601 eggs Nutrition 0.000 title claims abstract description 24
- 230000001939 inductive effect Effects 0.000 title claims description 15
- 241000255749 Coccinellidae Species 0.000 title claims description 13
- 241000131068 Coccinella septempunctata Species 0.000 claims abstract description 45
- 230000017448 oviposition Effects 0.000 claims abstract description 33
- 241000219843 Pisum Species 0.000 claims abstract description 28
- 241001124076 Aphididae Species 0.000 claims abstract description 18
- 241000238631 Hexapoda Species 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims abstract description 15
- 229920000742 Cotton Polymers 0.000 claims description 11
- 238000005192 partition Methods 0.000 claims description 9
- 239000003755 preservative agent Substances 0.000 claims description 5
- 230000002335 preservative effect Effects 0.000 claims description 5
- 241000131066 Coccinella Species 0.000 abstract description 17
- 230000000694 effects Effects 0.000 abstract description 15
- 238000000034 method Methods 0.000 abstract description 12
- 238000011084 recovery Methods 0.000 abstract description 5
- 230000010415 tropism Effects 0.000 abstract description 2
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000000843 powder Substances 0.000 description 13
- 229920002472 Starch Polymers 0.000 description 9
- 244000269722 Thea sinensis Species 0.000 description 9
- 235000013339 cereals Nutrition 0.000 description 8
- 239000011159 matrix material Substances 0.000 description 8
- 235000019698 starch Nutrition 0.000 description 7
- 239000008107 starch Substances 0.000 description 7
- 235000013616 tea Nutrition 0.000 description 7
- 238000011160 research Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000009395 breeding Methods 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 241000253994 Acyrthosiphon pisum Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
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- 235000006468 Thea sinensis Nutrition 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 235000009569 green tea Nutrition 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000020333 oolong tea Nutrition 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 229940100445 wheat starch Drugs 0.000 description 2
- 235000020334 white tea Nutrition 0.000 description 2
- 241000254173 Coleoptera Species 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010021033 Hypomenorrhoea Diseases 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 240000006677 Vicia faba Species 0.000 description 1
- 235000010749 Vicia faba Nutrition 0.000 description 1
- 235000002098 Vicia faba var. major Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
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- 230000007547 defect Effects 0.000 description 1
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- 210000005239 tubule Anatomy 0.000 description 1
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Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of insect cultivation, and particularly relates to a device for trapping coccinella septempunctata to lay eggs. According to the device for trapping coccinella septempunctata to lay eggs, pea seedlings planted with sufficient pea tailbiting aphids are used as trapping materials, the coccinella septempata and the pea seedlings carrying sufficient pea tailbiting aphids are placed in a spawning container to be trapped and spawned by utilizing the tropism of the coccinella septempata to aphids, so that the device has obvious trapping effect, can trap the coccinella septentata together, and is favorable for gathering and spawning; and the smooth black PE bag with the thickness of 0.1-0.2mm and high egg laying quantity is used as an egg laying carrier, so that the egg laying rate and recovery rate of the coccinella septempunctata are effectively improved, the problems of low egg laying amount and low egg recovery rate of the coccinella septempunctata are effectively solved, and the method has an important effect on ensuring population reproduction of the coccinella septempunctata.
Description
Technical Field
The invention belongs to the technical field of insect cultivation, and particularly relates to a device for trapping coccinella septempunctata to lay eggs.
Background
Aphids are extremely harmful pests in agriculture, bring irreversible damage to plants and the environment, and cause disasters in greenhouses and fields. At present, the prevention and treatment of aphids mainly comprises pesticide prevention and treatment, and although the effect is obvious, the method inevitably causes great chemical pollution caused by over-high dosage of chemical pesticides and causes secondary harm.
Coccinella septempunctata L is one of dominant natural enemies of aphids, and Coccinella septemfasciata belongs to Coleoptera, Coccinellidae and is an important predatory natural enemy of aphids on main crops such as wheat, cotton, oil and vegetables. Domestic and foreign researches prove that the method for treating aphids by using the coccinella septempunctata is an excellent biological control mode. The biological control measures for controlling the pests by the pests can effectively reduce the pollution to the environment and the harm to the human health caused by applying chemical pesticides, and have important ecological significance. With the improvement of the commercial application degree of biological control, researches on the aspects of growth and development, population large-scale propagation, application safety and the like of predatory ladybug have become new research hotspots.
The existing indoor artificial breeding method of the coccinella septempunctata has no mature system method, and the coccinella septempunctata can be stably propagated by subculture with good characters. The problems that the coccinella septempunctata can complete life history, but the fecundity is not strong, the egg laying method is lack of research, and mass subculture can not be realized always exist in the current method. The test ladybug of the coccinella septempunctata depends on field collection, and a stable insect source for artificial continuous multi-generation feeding is not available, so that research work on the coccinella septempunctata is limited, and the coccinella septempunctata as a natural enemy insect is also limited to be released and applied in fields.
Earlier stage researches show that the method for breeding the coccinella septempunctata by adopting indoor artificial breeding of the aphids of the broad beans is a better method for breeding the coccinella septempunctata. Wherein, factors such as environmental light period, different environmental colors and the like have obvious influence on the egg laying habit and the egg laying amount of the coccinella septempunctata. The device capable of trapping the coccinella septempunctata to effectively and intensively lay eggs plays an important role in large-scale artificial propagation of the coccinella septentata and provides a premise for the application of the coccinella septentata in fields.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to provide a device for inducing the coccinella septempunctata to lay eggs.
In order to solve the technical problem, the device for inducing the coccinella septempunctata to lay eggs comprises an open egg laying container, wherein a partition plate with an opening is arranged in the egg laying container, so that the egg laying container is divided into an upper layer inducing space and a lower layer egg laying space;
a funnel-shaped insect trap is arranged in the upper trapping space, an attracting core containing trapping materials is arranged at the open end of the insect trap, and the thin tube end of the insect trap penetrates through the opening to extend into the spawning space;
and a spawning carrier is laid in the lower spawning space.
The trapping material is pea seedlings carrying pea aphids for repairing tail.
And the root of the pea seedling is provided with soaked absorbent cotton.
The outer layer of the soaked absorbent cotton is coated with a preservative film.
The number of the pea tailbiting aphids is 180-.
The spawning carrier is a dark PE bag.
The spawning carrier is a black PE bag.
The spawning carrier is a black smooth PE bag.
The thickness of the spawning carrier is 0.1-0.2 mm.
The ratio of the input amount of the pea aphids for repairing tail to the number of the ladybug to be induced and collected for laying is 500-600: 1.
in order to further improve the trapping effect on the egg laying of the coccinella septempunctata, the trapping core of the invention also comprises a trapping matrix besides the trapping material;
specifically, the trapping matrix comprises tea powder, grain starch, water and agar, wherein the mass ratio of the tea powder to the grain starch to the water to the agar is 10-15: 8-12: 100: 2.
preferably, the mass ratio of the tea powder to the grain starch to the water is 12: 10: 100: 2.
specifically, the tea powder comprises green tea powder, white tea powder and/or oolong tea powder.
Preferably, the tea powder comprises the following components in percentage by mass: 1: 1 green tea powder, white tea powder and oolong tea powder.
In particular, the cereal starch comprises corn starch and/or wheat starch.
Preferably, the cereal starch comprises the following components in a mass ratio of 1: 1 and wheat starch.
The invention also discloses a method for preparing the trapping matrix, which comprises the step of uniformly mixing selected amounts of the tea powder, the grain starch, the water and the agar.
According to the device for trapping coccinella septempunctata to lay eggs, pea seedlings loaded with sufficient pea tailorage aphids are used as trapping materials, the coccinella septempunctata is placed in a spawning container to be trapped and spawned by utilizing the tropism of the coccinella septentata to aphids, and the device has obvious trapping effect, can trap the coccinella septentata together and is beneficial to the gathering and spawning of the coccinella septentata; and the smooth black PE bag with the thickness of 0.1-0.2mm and high egg laying quantity is used as an egg laying carrier, so that the egg laying rate and recovery rate of the coccinella septempunctata are effectively improved, the problems of low egg laying amount and low egg recovery rate of the coccinella septempunctata are effectively solved, and the method has an important effect on ensuring population reproduction of the coccinella septempunctata.
According to the core trapping material of the trapping device, the growth state of the pea seedlings has important influence on the number and the survival rate of the coccinella pisifolia, the number of the coccinella pisifolia affects the gathering effect and the egg laying amount of ladybirds, and in view of the fact that humidity has a decisive effect on keeping the freshness of the pea seedlings, the method for wrapping the roots of the pea seedlings of the trapping material after being soaked in absorbent cotton is adopted, and then a layer of preservative film is wrapped to prevent moisture on the absorbent cotton from evaporating so as to guarantee the healthy growth of the pea seedlings, so that the population stability of the aphids on the pea seedlings is guaranteed.
In order to improve the trapping effect of the device, the trapping matrix which is helpful for improving the trapping effect is further added into the trapping core, the trapping matrix takes selected tea powder and grain starch as raw materials, water and agar are added to prepare a solid matrix, and the olfactory effect of the adult coccinella septempunctata is utilized to effectively improve the trapping effect of the coccinella septempunctata.
The device for trapping the coccinella septempunctata to lay eggs integrates the color-trending effect and the olfactory effect of the adult coccinella septentata, provides a new method for trapping and laying eggs, can ensure that all the adult coccineata can lay eggs in a centralized way, reduces the eggs laid by the coccinella septentatively at positions which are not easy to recover by using the egg laying mediator and the adult coccineata trapping device in a matching way, improves the recovery rate of laying eggs of the adult coccineata, effectively solves the defects that the conventional method for laying eggs of the coccinella septentatively is labor-consuming and can not be recycled, and provides a new way for realizing the industrialized production of the coccinella septempunctata.
Drawings
In order that the present disclosure may be more readily and clearly understood, the following detailed description of the present disclosure is provided in connection with specific embodiments thereof and the accompanying drawings, in which,
FIG. 1 is a schematic perspective view of the apparatus for inducing spawning of coccinella septempunctata according to the present invention;
FIG. 2 is a top view of the device for inducing spawning of coccinella septempunctata according to the invention;
FIG. 3 is a sectional view taken along line A-A of FIG. 2;
FIG. 4 is a perspective view of the three-dimensional structure of the device for inducing the spawning of the coccinella septempunctata of the invention;
FIG. 5 is a front view of the device for inducing spawning of coccinella septempunctata according to the invention;
the reference numbers in the figures denote: 1-spawning container, 2-partition board, 3-opening hole, 4-insect trap, 5-lure core, 6-lure collecting space, 7-spawning space and 8-spawning carrier.
Detailed Description
With the structure shown in fig. 1, the device for trapping coccinella septempunctata to lay eggs comprises an open transparent spawning container 1, wherein a partition plate 2 with an opening 3 is arranged in the spawning container 1, so that the spawning container 1 is divided into an upper trapping space 6 and a lower spawning space 7; the partition plate 2 is preferably a transparent partition plate, and the partition plate 2 is preferably provided near the upper portion of the spawning container 1 to leave a sufficient space for the lower spawning space 7. The opening 3 of the partition 2 is preferably provided at a central position of the partition 2.
A funnel-shaped insect trap 4 is arranged in the upper trapping space 6, an attracting core 5 containing trapping materials is arranged at the open end of the insect trap 4, and the thin tube end of the insect trap 4 penetrates through the opening 3 and extends into the spawning space; the aperture of the opening 3 is suitable for the diameter of the tubule end of the insect trap 4. Lure core 5 to fix through modes such as support or couple, iron wire in lure worm ware 4 leaks hopper-shaped hollow, in the structure shown in figure 1, lure core 5 to be through the mode that the iron wire ties up promptly, with its be fixed in lure worm ware 4's hopper-shaped hollow, effectively ensured the ladybug of seven gets into the space of lower floor's oviposition space 7.
The trapping material is preferably fresh pea seedlings carrying pea tailbiting aphids, the fresh pea seedlings growing to about 10cm and having good growth vigor are selected, aphids are picked to the pea seedlings according to a proportion (about 200 heads of each pea seedling) by a writing brush and are pulled up along with roots, absorbent cotton is used for soaking to surround the roots of the pea seedlings, and a preservative film is covered on the absorbent cotton for moisture preservation, so that the trapping material is prepared. The ratio of the input amount of the pea aphids for repairing tail to the number of the ladybug to be induced and collected for laying is 500-600: 1.
in order to further improve the trapping effect on the egg laying of the coccinella septempunctata, the trapping core of the invention also comprises a trapping matrix besides the trapping material; specifically, the trapping matrix is prepared from the following components in a mass ratio of 10-15: 8-12: 100: 2, perforating the trapping substrate, enabling the roots of the pea seedlings to penetrate through the perforations of the solid substrate, soaking the roots of the pea seedlings with absorbent cotton to surround the roots of the pea seedlings, and covering the absorbent cotton with a preservative film for moisture preservation to obtain the trapping material.
In the induced spawning device, spawning carriers 8 are laid in the lower spawning space 7. The spawning carrier 8 is preferably a dark-colored PE bag, more preferably a black smooth PE bag, and is preferably laid to a thickness of 0.1-0.2 mm.
Examples 1 to 6
In the following examples of the invention, the egg laying trapping of coccinella septempunctata is carried out by using the trapping device shown in fig. 1 by respectively selecting the trapping cores of different trapping materials and trapping matrixes (see table 1 below). The trapping device is arranged in an insect breeding cage filled with 50 pairs of adult coccinella septempunctata 7 days after eclosion, is flush with the ground, and cleans the food of the coccinella septentata.
After 24 hours, the egg laying carrier arranged at the bottom layer of the insect cage is taken out, the PE bag attached with the egg mass is cut off by scissors, the cut PE bag is arranged in a culture dish with filter paper, water is added for moisture preservation, the culture and incubation are carried out under the conditions of 25 ℃ and 60-80% of relative humidity, the number of ladybug eggs on the egg laying carrier is counted, and the counting result is recorded in the following table 1.
TABLE 1 example ladybug egg laying number
Comparative example 1
The structure of the induced spawning device in this comparative example was the same as that of example 2 except that the spawning carrier was black printing paper. The egg laying amount is 378 grains.
Comparative example 2
The structure of the induced spawning device in this comparative example is the same as that of example 2, except that the spawning carrier is old newspaper. The egg laying amount was determined to be 332 pellets.
The data in the table show that the device for inducing the coccinella septempunctata to lay eggs can effectively induce the coccinella septempata to lay eggs, and particularly the setting of the inducing medium can effectively improve the egg laying amount of the coccinella septentata.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (7)
1. The ladybug egg-laying device is characterized by comprising an open egg-laying container (1), wherein a partition plate (2) with an opening (3) is arranged in the egg-laying container (1), so that the egg-laying container (1) is divided into an upper layer trapping space (6) and a lower layer egg-laying space (7);
a funnel-shaped insect trap (4) is arranged in the upper trapping space (6), an attracting core (5) containing trapping materials is arranged at the open end of the insect trap (4), and the thin tube end of the insect trap (4) penetrates through the opening (3) and extends into the spawning space;
the trapping material is pea seedlings carrying pea tailbiting aphids, water-soaked absorbent cotton is arranged at the roots of the pea seedlings, and the outer layer of the water-soaked absorbent cotton is coated with a preservative film;
and a spawning carrier (8) is laid in the lower spawning space (7).
2. The device for trapping egg laying of seven-star ladybug as claimed in claim 1, wherein the number of said pea tailgating aphids is 180-220 heads per pea seedling.
3. The device for inducing spawning of coccinella septempunctata according to claim 2, wherein the spawning carrier (8) is a dark PE bag.
4. The apparatus for inducing spawning of coccinella septempunctata according to claim 3, wherein the spawning carrier (8) is a black PE bag.
5. The device for inducing spawning of coccinella septempunctata according to claim 4, wherein the spawning carrier (8) is a black smooth PE bag.
6. The apparatus for inducing spawning of coccinella septempunctata according to claim 5, wherein the thickness of the spawning carrier (8) is 0.1-0.2 mm.
7. The device for trapping coccinella septempunctata to lay eggs according to any one of claims 1-6, wherein the ratio of the input amount of the aphid tailorae peasants to the number of coccinella septempunctata to be trapped to lay eggs is 500-600: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910403928.7A CN110122424B (en) | 2019-05-15 | 2019-05-15 | Device for inducing ladybug to lay eggs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910403928.7A CN110122424B (en) | 2019-05-15 | 2019-05-15 | Device for inducing ladybug to lay eggs |
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| Publication Number | Publication Date |
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| CN110122424A CN110122424A (en) | 2019-08-16 |
| CN110122424B true CN110122424B (en) | 2021-10-26 |
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| CN201910403928.7A Expired - Fee Related CN110122424B (en) | 2019-05-15 | 2019-05-15 | Device for inducing ladybug to lay eggs |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201869676U (en) * | 2010-11-05 | 2011-06-22 | 湖南省水稻研究所 | Efficient separation device for beneficial and injurious insects |
| CN202340662U (en) * | 2011-11-25 | 2012-07-25 | 刘春晓 | Mosquito and egg inducing device |
| CN104957116A (en) * | 2015-07-08 | 2015-10-07 | 山东省农业科学院植物保护研究所 | Method for luring and collecting Chinese chive maggot imagoes for laying eggs |
| CN105028345A (en) * | 2015-09-08 | 2015-11-11 | 山东省农业科学院植物保护研究所 | Breeding method for propylea japonica |
| CN205213898U (en) * | 2015-11-05 | 2016-05-11 | 山东省农业科学院植物保护研究所 | Device of laying eggs of moire ladybug |
| CN109221026A (en) * | 2018-11-20 | 2019-01-18 | 山东农业大学 | A kind of efficient device and method for collecting Bradysia odoriphaga ovum |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20140147943A (en) * | 2013-06-19 | 2014-12-31 | 공석태 | cc |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201869676U (en) * | 2010-11-05 | 2011-06-22 | 湖南省水稻研究所 | Efficient separation device for beneficial and injurious insects |
| CN202340662U (en) * | 2011-11-25 | 2012-07-25 | 刘春晓 | Mosquito and egg inducing device |
| CN104957116A (en) * | 2015-07-08 | 2015-10-07 | 山东省农业科学院植物保护研究所 | Method for luring and collecting Chinese chive maggot imagoes for laying eggs |
| CN105028345A (en) * | 2015-09-08 | 2015-11-11 | 山东省农业科学院植物保护研究所 | Breeding method for propylea japonica |
| CN205213898U (en) * | 2015-11-05 | 2016-05-11 | 山东省农业科学院植物保护研究所 | Device of laying eggs of moire ladybug |
| CN109221026A (en) * | 2018-11-20 | 2019-01-18 | 山东农业大学 | A kind of efficient device and method for collecting Bradysia odoriphaga ovum |
Non-Patent Citations (1)
| Title |
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| 环境颜色对七星瓢虫产卵的影响;肖达等;《中国生物防治学报》;20170123;第33卷(第1期);第44-48页 * |
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Inventor after: Zhou Xianhong Inventor after: Xia Xiaoju Inventor after: Zhuang Qianying Inventor after: Zhang Ansheng Inventor after: Xing Yuan men Inventor after: Li Lili Inventor after: Zhang Sicong Inventor after: Yu Yi Inventor before: Zhang Sicong Inventor before: Zhou Xianhong Inventor before: Xia Xiaoju Inventor before: Zhuang Qianying Inventor before: Zhang Ansheng Inventor before: Yu Yi Inventor before: Xing Yuan men Inventor before: Li Lili |
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Granted publication date: 20211026 |
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