CN110122235A - A method of greatly improving content of the total saponins in radix notoginseng - Google Patents
A method of greatly improving content of the total saponins in radix notoginseng Download PDFInfo
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- CN110122235A CN110122235A CN201910442278.7A CN201910442278A CN110122235A CN 110122235 A CN110122235 A CN 110122235A CN 201910442278 A CN201910442278 A CN 201910442278A CN 110122235 A CN110122235 A CN 110122235A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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Abstract
The present invention discloses a kind of method for greatly improving content of the total saponins in radix notoginseng, select well developed root system, disease-free, undamaged annual Radix Notoginseng as seedling, it is handled before transplanting using the infiltration of one or more of ethanol solution, choline chloride aqueous solution or the naphthalene acid solution of methyl jasmonate, 10-15 days after transplanting, plants probiotics are poured into the soil around transplanted seedling, after transplanting 1 year, 20-30 times of liquid of crown gall spirit or anti-2-4 times of liquid of crown gall microbial inoculum were sprayed around plant root every 2 months；After transplanting 2 years, salicylic acid solution and paclobutrazol aqueous solution are uniformly sprayed to blade face every other month, the amount of spraying is subject to that blade face is wet, dripless is dripped；5 months before harvesting, potassium fulvate is applied.Method of the invention can promote the growth of Radix Notoginseng main root, and main root is made to increase weight, and can significantly improve content of the total saponins in radix notoginseng, reduces root rot disease incidence, improves the utilization rate of fertilizer, reduce cost.Method of the invention is easy to grasp, benefit is evident, is suitable for promoting.
Description
Technical field
The present invention discloses a kind of method for greatly improving content of the total saponins in radix notoginseng, belongs to technical field of medicinal plant cultivation.
Background technique
Radix Notoginseng is Araliaceae Panax herbaceos perennial, is used as medicine with its dried root, has the two-way tune of hemostasis promoting blood circulation
Function is saved, is usually used in treating traumatic injury, sore and toxic, cardiovascular and cerebrovascular and enterogastric diseases etc..Saponin(e is the main medicine of Radix Notoginseng
It is the triterpenes secondary metabolites based on carbon skeleton structure with ingredient and quality evaluation index.2015 editions " the Chinese people are total
With state's pharmacopeia " regulation, pseudo-ginseng is " panax araliaceae plantPanaxnotoginseng(Burk.)F.H.ChenDrying
Root and rhizome ", alsies specify that " this product is calculated by dry product containing ginsenoside Rg1 (C42H72O14), ginsenoside Rb1
(C54H92O23) and the total amount of notoginsenoside R (C47H80O18) must not be less than 5.0% ".
The field production that notoginsenoside mainly passes through Radix Notoginseng obtains.But due to the particularity of Panax notoginseng Growth environment and kind
Unicity causes notoginseng obstacle extremely serious, planted Radix Notoginseng plot usually need crop rotation 8-10 after can plant again so that
The land area that Radix Notoginseng can be cultivated increasingly is reduced.In addition the continuous development of Radix Notoginseng industry in recent years, demand of the market to Radix Notoginseng
Constantly increase, cultivation of pseudo-ginseng has been showed to be shifted from Genuine producing area to non-Genuine producing area, causes the Radix Notoginseng sold on current market
Medicinal ingredient is low compared with Genuine producing area, and even lower than the Pharmacopoeia of the People's Republic of China provides.
Therefore, it needs to research and develop a kind of cultural method for greatly improving content of the total saponins in radix notoginseng, improves Radix Notoginseng medicinal ingredient and contain
Amount, and then improve the quality of Radix Notoginseng.
Summary of the invention
To solve the above problems, the present invention provides a kind of method for greatly improving content of the total saponins in radix notoginseng, this method operation
Simply, it is easy to grasp, is suitable for promoting.
The present invention is realized by following technical proposal: a method of content of the total saponins in radix notoginseng being greatly improved, including following
Each operation:
(1) select well developed root system, disease-free, undamaged annual Radix Notoginseng as seedling, it is 1-6mg/L that concentration is used before transplanting
The ethanol solution of methyl jasmonate, concentration are 1000-5000mg/L choline chloride aqueous solution, concentration is 10 ~ 20mg/kg methyl α-naphthyl acetate
One of solution or arbitrary proportion are several, infiltrate seedling 2-5 minutes；It can promote Radix Notoginseng using choline chloride solution infiltration seedling
Seedling root of hair and early stage root tuber are expanded；Methyl α-naphthyl acetate can effectively facilitate plant root growth, promote that branches and leaves are luxuriant, robust plant, also
Crop cold resistance, drought resisting, waterlogging-resistant, disease-resistant, salt resistance alkali, anti-adversity ability can be effectively improved；Methyl jasmonate can safely and effectively swash
Send out the generation and accumulation of Plant Secondary Metabolites；
(2) 10-15 days after transplant step (1) seedling are diluted with water to by plants probiotics by the amount of 200-300g per acre
It is poured into the soil around transplanted seedling after 300 times of liquid, holding illumination light transmittance is 15-30%, and is routinely poured；The plant
Probiotics is photosynthetic bacteria group, lactobacillus, yeast flora, bacillus, acetic acid bacteria, Bifidobacterium, nitrobacteria, gram sun
Property actinomycete group, fermentation system Filamentous flora in arbitrary proportion two or more mixture；
(3) after transplanting 1 year, 20-30 times of liquid of crown gall spirit or 2-4 times of crown gall microbial inoculum anti-was sprayed around plant root every 2 months
Liquid；
(4) transplant 2 years after, and in excavation preceding 8 months, every other month to the uniform spraying concentration in blade face for 1000mg/L~
The salicylic acid solution and concentration of 1500mg/L is the mixed liquor of the paclobutrazol aqueous solution of 600mg/L~800mg/L, and salicylic acid is molten
The dosage volume ratio of liquid and paclobutrazol aqueous solution is 1:1~1:2, and the amount of spraying is subject to that blade face is wet, dripless is dripped；And/or
The paclobutrazol aqueous solution that person is every other month 400mg/L~800mg/L with concentration, dosage is 20000~30000L/ mus fills
Root；
(5) 5 months before harvesting, the potassium fulvate of 20~70kg of dosage per acre is taken, is carried out after being diluted to 300-600 times of liquid with water
It pours.
300 times of liquid of the step (2) are the dilutions that effective microbe content is hundred million cfu/ml of 200-500.
Photosynthetic bacteria group, lactobacillus, yeast flora, bacillus, the acetic acid bacteria, Bifidobacterium, nitrification of the step (2)
The Filamentous flora that bacterium, Gram positive actinomycetes group, fermentation are is regular market purchase product.
The concentration of the step (4) is to be additionally added first piperazine in the paclobutrazol aqueous solution of 400mg/L~800mg/L, make first
The concentration of piperazine is 600mg/L~800mg/L.
The advantages of present invention has and effect: method of the invention can promote the growth of Radix Notoginseng main root, so that main root is increased weight, root system
Cell wall thickening, excitation secondary metabolite largely generate, and content of the total saponins in radix notoginseng can be significantly improved, to improve Radix Notoginseng etc.
Grade.Radix Notoginseng is planted with the inventive method, can greatly reduce plant pest and disease damage, reduces notoginseng root rot disease incidence；This method
It can effectively improve soil nutrient supply situation, promote the release of nutrient, be provided with function source for edaphon, improve root
Border microenvironment promotes edaphon and enzymatic activity, and then improves Radix Notoginseng root activity, promotes to grow and improves yield；Simultaneously
The utilization rate of Radix Notoginseng fertilizer is also improved, fertilizer is avoided to waste, is reduced environmental pollution, cost is substantially reduced.Method of the invention
Be easy to grasp, benefit is evident, be suitable for promote.
Specific embodiment
Below with reference to embodiment, the present invention will be further described.
Embodiment 1
(1) select well developed root system, disease-free, undamaged annual Radix Notoginseng as seedling, it is 6mg/L jasmine that concentration is used before transplanting
The ethanol solution of jasmine acid methyl esters, the mixed liquor that concentration is 1000mg/L choline chloride aqueous solution, infiltrate seedling 3 minutes；
(2) 12 days after transplant step (1) seedling are diluted with water to 300 times of liquid and pour by plants probiotics by the amount of 280g per acre
Enter in the soil around transplanted seedling, keeping illumination light transmittance is 15%, and is routinely poured；The plants probiotics are photosynthetic bacterias
The Filamentous flora that group, bacillus, Gram positive actinomycetes group and fermentation are presses the mixture of 1: 1: 1: 1 volume ratio mixing；
300 times of liquid is the dilution that effective microbe content is hundred million cfu/ml of 200-500；
(3) after transplanting 1 year, the clever 30 times of liquid of crown gall were sprayed around plant root every 2 months；
It (4) is every other month 1000mg/L's to the uniform spraying concentration in blade face after transplanting 2 years, and in first 8 months of excavation
Salicylic acid solution, the amount of spraying are subject to that blade face is wet, dripless is dripped；It is simultaneously 800mg/L with concentration, dosage 20000L/
The water-soluble liquid irrigating root of paclobutrazol of mu；
(5) 5 months before harvesting, the potassium fulvate of dosage 50kg per acre is taken, is poured after being diluted to 400 times of liquid with water.
Embodiment 2
(1) select well developed root system, disease-free, undamaged annual Radix Notoginseng as seedling, it is 1mg/L jasmine that concentration is used before transplanting
The mixing that the ethanol solution of jasmine acid methyl esters, concentration are 5000mg/L choline chloride aqueous solution, concentration is 20mg/kg naphthalene acid solution
Object infiltrates seedling 2 minutes；
(2) 10 days after transplant step (1) seedling, by plants probiotics by the amount of 200g per acre, after being diluted with water to 300 times of liquid
It pours into the soil around transplanted seedling, keeping illumination light transmittance is 30%, and is routinely poured；The plants probiotics are lactic acid
The mixture that flora, yeast flora, acetic acid bacteria, Bifidobacterium, nitrobacteria are mixed by 1: 2: 1: 2: 1 volume ratio；Described 300
Times liquid is the dilution that effective microbe content is hundred million cfu/ml of 200-500；
(3) after transplanting 1 year, 4 times of liquid of anti-crown gall microbial inoculum were sprayed around plant root every 2 months；
It (4) is every other month 400mg/L with concentration, dosage 30000L/ after transplanting 2 years, and in first 8 months of excavation
The water-soluble liquid irrigating root of paclobutrazol of mu, the first piperazine for being wherein 800mg/L containing concentration in paclobutrazol aqueous solution；
(5) 5 months before harvesting, the potassium fulvate of dosage 70kg per acre is taken, is poured after being diluted to 600 times of liquid with water.
Embodiment 3
(1) select well developed root system, disease-free, undamaged annual Radix Notoginseng as seedling, it is 4000mg/ that concentration is used before transplanting
L choline chloride solution immersion seedling 5 minutes；
(2) 10 days after transplant step (1) seedling, by plants probiotics by the amount of 220g per acre, after being diluted with water to 300 times of liquid
It pours into the soil around transplanted seedling, keeping illumination light transmittance is 25%, and is routinely poured；The plants probiotics are gemma
The mixture for the Filamentous flora 2: 1: 2 that bacillus, Bifidobacterium, fermentation are；300 times of liquid is that effective microbe content is 200-
The dilution of 50000000000 cfu/ml；
(3) after transplanting 1 year, 3 times of liquid of anti-crown gall microbial inoculum were sprayed around plant root every 2 months；
It (4) is every other month 800mg/L with concentration, dosage 25000L/ after transplanting 2 years, and in first 8 months of excavation
The water-soluble liquid irrigating root of paclobutrazol of mu, the first piperazine for being wherein 600mg/L containing concentration in paclobutrazol aqueous solution；
(5) 5 months before harvesting, the potassium fulvate of dosage 60kg per acre is taken, is poured after being diluted to 300 times of liquid with water.
Embodiment 4
(1) select well developed root system, disease-free, undamaged annual Radix Notoginseng as seedling, it is 3mg/L jasmine that concentration is used before transplanting
The ethanol solution of jasmine acid methyl esters, concentration are 3000mg/L choline chloride aqueous solution, concentration is the mixing of 10mg/kg naphthalene acid solution,
Infiltration seedling 5 minutes；
(2) 15 days after transplant step (1) seedling, by plants probiotics by the amount of 300g per acre, after being diluted with water to 300 times of liquid
It pours into the soil around transplanted seedling, keeping illumination light transmittance is 20%, and is routinely poured；The plants probiotics are bifids
1: 2 mixture of the Filamentous flora that bacillus, fermentation are；300 times of liquid is that effective microbe content is hundred million cfu/ml of 200-500
Dilution；
(3) after transplanting 1 year, 2 times of liquid of anti-crown gall microbial inoculum were sprayed around plant root every 2 months；
It (4) is every other month 1500mg/L's to the uniform spraying concentration in blade face after transplanting 2 years, and in first 8 months of excavation
The paclobutrazol aqueous solution that salicylic acid solution and concentration are 600mg/L, and the dosage volume of salicylic acid solution and paclobutrazol aqueous solution
Than for 1:1, the amount of spraying is subject to that blade face is wet, dripless is dripped；
(5) 5 months before harvesting, the potassium fulvate of dosage 20kg per acre is taken, is poured after being diluted to 400 times of liquid with water.
Comparative example 1: with embodiment 1, only " use the ethyl alcohol that concentration is 6mg/L methyl jasmonate molten before transplanting step (1)
Liquid and concentration are the mixing of 1000mg/L choline chloride aqueous solution, infiltration seedling 3 minutes " it omits.
Comparative example 2: with embodiment 1, only step (2) are replaced are as follows: after seedling transplanting, the nitrogenous fertilizer of 1:2 and potassium in mass ratio
Fertilizer applies fertilizer to seedling.
Comparative example 3: with embodiment 1, step (4) only are omitted.
Comparative example 4: with embodiment 1, step (5) only are omitted.
Comparative example 5: with embodiment 1, step (6) only are omitted.
The same time limit Radix Notoginseng of general planting and embodiment Radix Notoginseng and comparative example notoginseng planting, in Wenshan Prefecture Yanshan County new people
It is completed with plantation in a piece of soil in village.
Conclusion: the Radix Notoginseng that the implantation methods invented above obtain, through detecting, spring content of the total saponins in radix notoginseng reaches 13.6-
15.3%, compared with the identical general planting method of implantation time, content of the total saponins in radix notoginseng improves 2 times or more；Winter arasaponin
Content reaches 10.7-11.5%, general planting method ratio identical with implantation time, and content of the total saponins in radix notoginseng improves 2 times or more,
Effect is quite apparent.
Claims (4)
1. a kind of method for greatly improving content of the total saponins in radix notoginseng, it is characterised in that including following each operation:
(1) select well developed root system, disease-free, undamaged annual Radix Notoginseng as seedling, it is 1-6mg/L that concentration is used before transplanting
The ethanol solution of methyl jasmonate, concentration are 1000-5000mg/L choline chloride aqueous solution, concentration is 10 ~ 20mg/kg methyl α-naphthyl acetate
One of solution or arbitrary proportion are several, infiltrate seedling 2-5 minutes；
(2) 10-15 days after transplant step (1) seedling are diluted with water to by plants probiotics by the amount of 200-300g per acre
It is poured into the soil around transplanted seedling after 300 times of liquid, holding illumination light transmittance is 15-30%, and is routinely poured；The plant
Probiotics is photosynthetic bacteria group, lactobacillus, yeast flora, bacillus, acetic acid bacteria, Bifidobacterium, nitrobacteria, gram sun
Property actinomycete group, fermentation system Filamentous flora in arbitrary proportion two or more mixture；
(3) after transplanting 1 year, 20-30 times of liquid of crown gall spirit or 2-4 times of crown gall microbial inoculum anti-was sprayed around plant root every 2 months
Liquid；
(4) transplant 2 years after, and in excavation preceding 8 months, every other month to the uniform spraying concentration in blade face for 1000mg/L~
The salicylic acid solution and concentration of 1500mg/L is the mixed liquor of the paclobutrazol aqueous solution of 600mg/L~800mg/L, and salicylic acid is molten
The dosage volume ratio of liquid and paclobutrazol aqueous solution is 1:1~1:2, and the amount of spraying is subject to that blade face is wet, dripless is dripped；And/or
The paclobutrazol aqueous solution that person is every other month 400mg/L~800mg/L with concentration, dosage is 20000~30000L/ mus fills
Root；
(5) 5 months before harvesting, the potassium fulvate of 20~70kg of dosage per acre is taken, is carried out after being diluted to 300-600 times of liquid with water
It pours.
2. the method according to claim 1 for greatly improving content of the total saponins in radix notoginseng, it is characterised in that: the step (2)
300 times of liquid be effective microbe content be hundred million cfu/ml of 200-500 dilution.
3. the method according to claim 1 for greatly improving content of the total saponins in radix notoginseng, it is characterised in that: the step (2)
Photosynthetic bacteria group, lactobacillus, yeast flora, bacillus, acetic acid bacteria, Bifidobacterium, nitrobacteria, Gram-positive unwrapping wire
The Filamentous flora that flora, fermentation are is regular market purchase product.
4. the method according to claim 1 for greatly improving content of the total saponins in radix notoginseng, it is characterised in that: the step (4)
Concentration be 400mg/L~800mg/L paclobutrazol aqueous solution in be additionally added first piperazine, make the concentration 600mg/L of first piperazine
~800mg/L.
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Cited By (2)
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|---|---|---|---|---|
| CN112205406A (en) * | 2020-10-24 | 2021-01-12 | 云南农业大学 | Method for rapidly increasing saponin content of panax notoginseng leaf |
| CN113598003A (en) * | 2021-09-15 | 2021-11-05 | 昆明理工大学 | Method for improving synthesis of notoginsenoside and application |
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| CN113598003A (en) * | 2021-09-15 | 2021-11-05 | 昆明理工大学 | Method for improving synthesis of notoginsenoside and application |
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| CN110122235B (en) | 2021-07-06 |
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