CN112205406A - Method for rapidly increasing saponin content of panax notoginseng leaf - Google Patents
Method for rapidly increasing saponin content of panax notoginseng leaf Download PDFInfo
- Publication number
- CN112205406A CN112205406A CN202011150134.3A CN202011150134A CN112205406A CN 112205406 A CN112205406 A CN 112205406A CN 202011150134 A CN202011150134 A CN 202011150134A CN 112205406 A CN112205406 A CN 112205406A
- Authority
- CN
- China
- Prior art keywords
- spraying
- panax notoginseng
- content
- acid
- working solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/24—Cyanogen or compounds thereof, e.g. hydrogen cyanide, cyanic acid, cyanamide, thiocyanic acid
Abstract
The invention relates to a method for rapidly improving the content of notoginsenoside in leaves, belonging to the technical field of medicine. Firstly, preparing exogenous substance working solution; the exogenous substances are methyl jasmonate, naphthylacetic acid, 6-benzyladenine and H2O2Salicylic acid, spermidine or abscisic acid; the solvent of the exogenous substance working solution is water; then, the prepared working solution is adopted to spray the leaves of the pseudo-ginseng plants. The method utilizes the induction effect of exogenous substances on the accumulation of saponin monomers in the panax notoginseng leaves to screen out the optimal exogenous substances, and provides certain scientific basis and reference for improving the content of saponin in the panax notoginseng leaves and carrying out quality evaluation and quality analysis.
Description
Technical Field
The invention belongs to the technical field of medicine, and particularly relates to a method for rapidly increasing the content of arasaponin by using exogenous substances.
Background
The process of saponin accumulation in roots is long, and the exogenous substances acting on leaves have the characteristics of quick absorption, quick induction, quick accumulation and the like. Research shows that the exogenous substance can induce the secondary metabolism of medicinal plant widelyAccumulation of substances, but with different regulatory mechanisms. For example, exogenous NO donors Sodium Nitroprusside (SNP), hydrogen peroxide (H)2O2) Is beneficial to the synthesis of flavonoid and alkaloid substances in medicinal plants; the Jasmonic Acid (JAs) can induce the synthesis of flavonoid, terpenoid, alkaloid and other active ingredients in medicinal plants; abscisic acid (ABA), naphthylacetic acid (NAA), methyl jasmonate (MeJA) and Salicylic Acid (SA) have obvious effects on the growth of hairy roots of American ginseng (Panax quinquefolius L.) and the improvement of the content of triterpenoid saponin; methyl jasmonate (MeJA) is also used as a powerful and effective inducer to significantly improve the biosynthesis of triterpenoid saponins in ginseng (Panax ginseng C.A.Mey). For example, Yu (2002) and the like have found that protopanaxadiol-type ginsenoside Rb of ginseng adventitious roots after JA treatment1、Rb2The total amount of Rc is increased by more than 5 times, and the original panatriol ginsenoside Re and Rg1And Rf without a significant increase; oh (2014) and the like significantly increase protopanoxadiol-type ginsenosides at each part of the ginseng after soaking the root of the ginseng in MeJA. Therefore, JAs is effective in increasing ginsenoside content, especially protopanaxadiol type ginsenoside. Niu (2014) and the like spray 200 mu M methyl jasmonate on four-year-old panax notoginseng leaves, and the relative expression quantity of genes related to saponin is found to be increased, but the change of the saponin content is detected. The research explores the influence of MeJA on the content of notoginsenoside in leaves by using the successful experience of predecessor in other plants of Panax, and successfully obtains a method capable of rapidly improving the content of notoginsenoside in leaves.
Disclosure of Invention
The invention aims to solve the defects of the prior art and provide a method for rapidly improving the content of the arasaponin.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a method for rapidly improving the content of notoginsenosides in leaves comprises the following steps:
step (1), preparing exogenous substance working solution; the exogenous substances are methyl jasmonate, naphthylacetic acid, 6-benzyladenine and H2O2Salicylic acid, spermidineOr abscisic acid; the solvent of the exogenous substance working solution is water;
step (2), spraying the pseudo-ginseng plant leaves by adopting the working solution prepared in the step (1); continuously spraying for one week; the spraying season is 6-8 months per year, the spraying period is one week, the spraying amount of each plant is 1.35-2 ml/time in the morning and at night each day.
Further, preferably, the concentrations of methyl jasmonate, naphthylacetic acid, 6-benzyladenine, abscisic acid, salicylic acid, spermidine and abscisic acid working solution are all 400 mu mol/L; h2O2The concentration of the working solution was 20 mmol/L.
Further, preferably, the notoginseng plant is annual notoginseng.
Further, it is preferable that the two spraying times are 6 to 7 as early as and 7 to 8 as late as possible, respectively.
Further, it is preferable that the spraying range is 0.5m × 0.5m when spraying, the range contains 150 annual panax notoginseng 100-.
The invention also provides the application of the methyl jasmonate in preparing the medicine for improving the ginsenoside Rb in the panax notoginseng1Ginsenoside Rc and ginsenoside Rb2Ginsenoside Rb3And one or more saponin monomers in the contents of notoginsenoside Fa, notoginsenoside Fe and esculin XVII.
The invention also provides the naphthylacetic acid, 6-benzyladenine, abscisic acid and H2O2And salicylic acid or spermidine in the preparation of the medicament for improving the content of notoginsenoside Fe in the panax notoginseng.
The invention also provides application of the abscisic acid in preparation of a medicament for improving the content of esculin XVII in pseudo-ginseng.
The invention takes annual pseudo-ginseng as a research object, and gibberellin (GA3), 6-benzyladenine (6-BA), abscisic acid (ABA), methyl jasmonate (MeJA), Salicylic Acid (SA), spermidine (Spd), Sodium Nitroprusside (SNP), naphthylacetic acid (NAA), Ethylene (ETH), Melatonin (MT) and H are sprayed on leaf surfaces under the condition of artificial cultivation2O211 common exogenous substances are treated to establish high-efficiency liquidOn the basis of a method for measuring the monomer of the notoginsenoside by using phase chromatography (HPLC), the induction effect of the 11 exogenous substances on the accumulation of the monomer of the notoginsenoside in the pseudo-ginseng leaf is explored, the optimal exogenous substance is screened out, and certain scientific basis and reference are provided for improving the content of the saponin in the pseudo-ginseng leaf and carrying out quality evaluation and quality analysis.
Compared with the prior art, the invention has the beneficial effects that:
on the basis of establishing a method for determining the monomer of the notoginsenoside by High Performance Liquid Chromatography (HPLC), the invention explores the induction effect of 11 exogenous substances on the accumulation of the saponin monomer in the notoginsenoside, screens out the optimal exogenous substance, and provides certain scientific basis and reference for improving the content of the saponin in the notoginsenoside and carrying out quality evaluation and quality analysis.
The invention uses exogenous substances to spray annual pseudo-ginseng leaves, the period of the method is only one week, but a plurality of elicitors, namely methyl jasmonate, salicylic acid, naphthylacetic acid, 6-benzyladenine, gibberellin, abscisic acid, ethylene, melatonin, sodium nitroprusside, spermidine and H2O2The content of a plurality of saponin monomers in the annual leaf blade of the panax notoginseng is obviously improved (see table 2).
Detailed Description
The present invention will be described in further detail with reference to examples.
It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention. The examples do not specify particular techniques or conditions, and are performed according to the techniques or conditions described in the literature in the art or according to the product specifications. The materials or equipment used are not indicated by manufacturers, and all are conventional products available by purchase.
Example 1
A method for rapidly improving the content of notoginsenosides in leaves comprises the following steps:
step (1), preparing exogenous substance working solution; the exogenous substances are methyl jasmonate, naphthylacetic acid, 6-benzyladenine and H2O2Salicylic acid, spermidine or abscisic acid; the solvent of the exogenous substance working solution is water；
Step (2), spraying the pseudo-ginseng plant leaves by adopting the working solution prepared in the step (1); continuously spraying for one week; the spraying season is 6 months per year, the spraying period is one week, the spraying amount of each plant is 1.35 ml/time, and the spraying time is respectively in the morning and the evening of each day.
Example 2
A method for rapidly improving the content of notoginsenosides in leaves comprises the following steps:
step (1), preparing exogenous substance working solution; the exogenous substances are methyl jasmonate, naphthylacetic acid, 6-benzyladenine and H2O2Salicylic acid, spermidine or abscisic acid; the solvent of the exogenous substance working solution is water;
step (2), spraying the pseudo-ginseng plant leaves by adopting the working solution prepared in the step (1); continuously spraying for one week; the spraying season is 8 months per year, the spraying period is one week, the spraying amount of each plant is 2 ml/time, and the spraying time is respectively in the morning and the evening of each day.
The concentrations of methyl jasmonate, naphthylacetic acid, 6-benzyladenine, abscisic acid, salicylic acid, spermidine and abscisic acid working solution are all 400 mu mol/L; h2O2The concentration of the working solution was 20 mmol/L.
The notoginseng plant is annual notoginseng.
The spraying time of the two times is respectively 6-7 hours earlier and 7-8 hours later.
Example 3
A method for rapidly improving the content of notoginsenosides in leaves comprises the following steps:
step (1), preparing exogenous substance working solution; the exogenous substances are methyl jasmonate, naphthylacetic acid, 6-benzyladenine and H2O2Salicylic acid, spermidine or abscisic acid; the solvent of the exogenous substance working solution is water;
step (2), spraying the pseudo-ginseng plant leaves by adopting the working solution prepared in the step (1); continuously spraying for one week; the spraying season is 7 months per year, the spraying period is one week, the spraying amount of each plant is 1.5 ml/time, and the spraying time is respectively in the morning and the evening of each day.
Jasmonic acid methyl ester and naphthaleneThe concentrations of acetic acid, 6-benzyladenine, abscisic acid, salicylic acid, spermidine and abscisic acid working solution are all 400 mu mol/L; h2O2The concentration of the working solution was 20 mmol/L.
The notoginseng plant is annual notoginseng.
The spraying time of the two times is respectively 6-7 hours earlier and 7-8 hours later.
Example 4
A method for rapidly improving the content of notoginsenosides in leaves comprises the following steps:
step (1), preparing exogenous substance working solution; the exogenous substances are methyl jasmonate, naphthylacetic acid, 6-benzyladenine and H2O2Salicylic acid, spermidine or abscisic acid; the solvent of the exogenous substance working solution is water;
step (2), spraying the pseudo-ginseng plant leaves by adopting the working solution prepared in the step (1); continuously spraying for one week; the spraying season is 6-8 months per year, the spraying period is one week, the spraying amount of each plant is 1.35-2 ml/time, and the spraying time is respectively in the morning and at the evening.
The concentrations of methyl jasmonate, naphthylacetic acid, 6-benzyladenine, abscisic acid, salicylic acid, spermidine and abscisic acid working solution are all 400 mu mol/L; h2O2The concentration of the working solution was 20 mmol/L.
The notoginseng plant is annual notoginseng.
The spraying time of the two times is respectively 6-7 hours earlier and 7-8 hours later.
Examples of the applications
(1) Spraying Notoginseng radix with eleven exogenous substances, and setting a control group, spraying with distilled water;
the method comprises the following specific steps:
setting 12 treatment groups, wherein the area of each treatment sample is 0.5m multiplied by 0.5m, 100-150 annual panax notoginseng plants are arranged in the treatment groups, and the spraying ranges of the exogenous substance working solutions are mutually separated by 1-1.5 m. One group was selected as a blank Control (CK), and the other 11 groups were GA3, 6-BA, ABA, MeJA, SA, Spd, SNP, NAA, ETH, MT and H, respectively2O2(all purchased from Shanghai biological engineering with limited shares of resourcesCompany) treatment groups, each treatment group being set to 3 replicates. During the test, the exogenous substances are prepared into 1000 times of mother solution and stored in a refrigerator, and H is removed during the test2O2The solution was prepared in a proportion of 400. mu. mol/L in addition to 20 mmol/L. The experimental treatment is carried out on 6-14 th month in 2017, the spraying time is 6-7 hours in the morning and 7-8 hours in the evening, 200mL of different exogenous substance working solutions prepared from mother liquor are uniformly sprayed on annual pseudo-ginseng leaves of different treatment groups, and equal amount of distilled water is sprayed on a control group.
(2) Collecting and cleaning a panax notoginseng sample, and measuring the growth index of the panax notoginseng sample;
the measurement method is as follows:
precisely weighing 1g of milled folium Notoginseng powder in a 50ml conical flask, adding 50ml of chloroform, standing overnight (removing chlorophyll), filtering the solution with filter paper the next day, removing the filtrate, taking the filter paper for precipitation, naturally drying the precipitate, dissolving in 30ml of chloroform again (removing chlorophyll), standing for 2h, filtering with filter paper again, and naturally drying the precipitate. Then, a sample solution to be tested is prepared by referring to a preparation method of a pseudo-ginseng rhizome sample solution in the first part of the Chinese pharmacopoeia 2010 edition. The sample detection adopts an Agilent 1260 series high performance liquid chromatograph, and the instrument comprises: a G1311C quaternary pump, a G1329B autosampler, a G1315D photodiode array detector. Chromatographic determination conditions: the column was Agilent ZORBAX SB-C18(250 mm. times.4.6 mm, 5 μm); the mobile phase is 0.2 percent (volume ratio) of phosphoric acid water (A) -acetonitrile (B); flow rate 1.0 mL/min-1(ii) a The detection wavelength is 203 nm; the column temperature is 25 ℃; the injection volume is 10. mu.L.
Gradient elution is as in table 1.
TABLE 1
| Time (min) | Mobile phase B% |
| 0-28min | 27％B→34％B |
| 28-44min | 34％B→39％B |
| 44-47min | 39％B→60％B |
| 47-50min | 60％B→80％B |
| 50-52min | 80％B→85％B |
Note: the concentration change in each time period is a linear change.
(3) Determining an optimal exogenous substance;
the content of 7 kinds of arasaponin monomers after 11 kinds of exogenous substances are treated is measured. As shown in Table 2, the total content of 7 saponin monomers in the leaves of Panax notoginseng after MeJA treatment was significantly increased, while the total content of 7 saponin monomers was significantly reduced by the treatment of the remaining 10 exogenous substances, as compared to CK. MeJA treatment significantly increased ginsenoside Rb for each monomer content1、Rc、Rb2、Rb3The contents of notoginsenoside Fa, notoginsenoside Fe and esculin X VII; NAA, 6-BA, ABA, H2O2SNP and Spd treatment obviously increase the content of notoginsenoside Fe; ABA treatment obviously increases the content of esculin X VII; the rest treatment has no obvious or obviously reduced effect on the content of each saponin monomer. The result shows that MeJA has the most obvious effect of inducing the increase of the monomer content of the main saponin in the panax notoginseng leaves in the 11 applied exogenous substances.
TABLE 2
Note: correlation was significant at 0.01 (two-tailed) compared to CK only for each treatment; *. correlation at 0.05
Note: correlation was significant at 0.01 (two-tailed) compared to CK only for each treatment; *. correlation was significant at 0.05 (two-tailed).
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A method for rapidly improving the content of saponin in leaves of panax notoginseng is characterized by comprising the following steps:
step (1), preparing exogenous substance working solution; the exogenous substances are methyl jasmonate, naphthylacetic acid, 6-benzyladenine and H2O2Salicylic acid, spermidine or abscisic acid; the solvent of the exogenous substance working solution is water;
step (2), spraying the pseudo-ginseng plant leaves by adopting the working solution prepared in the step (1); continuously spraying for one week; the spraying season is 6-8 months per year, the spraying period is one week, the spraying amount of each plant is 1.35-2 ml/time in the morning and at night each day.
2. The method for rapidly increasing the content of notoginsenoside according to claim 1, wherein the concentrations of methyl jasmonate, naphthylacetic acid, 6-benzyladenine, abscisic acid, salicylic acid, spermidine and abscisic acid working solution are all 400 μmol/L; h2O2The concentration of the working solution was 20 mmol/L.
3. The method for rapidly increasing the saponin content of panax notoginseng leaf according to claim 1, wherein the panax notoginseng plant is annual panax notoginseng.
4. The method for rapidly increasing the saponin content of panax notoginseng leaf according to claim 1, wherein the two spraying times are 6 to 7 hours earlier and 7 to 8 hours later respectively.
5. The method for rapidly increasing the saponin content in leaves of Panax notoginseng as claimed in claim 1, wherein the spraying is performed in a range of 0.5m x 0.5m, which contains 100-150 annual Panax notoginseng plants, and the spraying amount is 200mL each time.
6. The use of methyl jasmonate as claimed in claim 1 for increasing ginsenoside Rb in Panax notoginseng1Ginsenoside Rc and ginsenoside Rb2Ginsenoside Rb3And one or more saponin monomers in the contents of notoginsenoside Fa, notoginsenoside Fe and esculin XVII.
7. The naphthylacetic acid, 6-benzyladenine, abscisic acid, H, as claimed in claim 12O2And salicylic acid or spermidine in the preparation of the medicament for improving the content of notoginsenoside Fe in the panax notoginseng.
8. Use of abscisic acid as defined in claim 1 in the manufacture of a medicament for increasing the content of esculin xvii in notoginseng radix.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011150134.3A CN112205406A (en) | 2020-10-24 | 2020-10-24 | Method for rapidly increasing saponin content of panax notoginseng leaf |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011150134.3A CN112205406A (en) | 2020-10-24 | 2020-10-24 | Method for rapidly increasing saponin content of panax notoginseng leaf |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112205406A true CN112205406A (en) | 2021-01-12 |
Family
ID=74055062
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011150134.3A Withdrawn CN112205406A (en) | 2020-10-24 | 2020-10-24 | Method for rapidly increasing saponin content of panax notoginseng leaf |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112205406A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931079A (en) * | 2021-02-24 | 2021-06-11 | 成都医学院 | Method for improving quality of selfheal medicinal materials by spraying exogenous hormones |
| CN113598003A (en) * | 2021-09-15 | 2021-11-05 | 昆明理工大学 | Method for improving synthesis of notoginsenoside and application |
| CN115500168A (en) * | 2022-10-20 | 2022-12-23 | 中南林业科技大学 | Method for regulating camellia oleifera gene by using methyl jasmonate |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104920214A (en) * | 2015-06-03 | 2015-09-23 | 中国科学院昆明植物研究所 | Method for improving yield of saponin in panax notoginseng tissue culture seedlings |
| CN105052581A (en) * | 2015-08-05 | 2015-11-18 | 广西壮族自治区药用植物园 | Method for improving ginsenoside Rg1 content in pseudo-ginseng |
| CN110122235A (en) * | 2019-05-24 | 2019-08-16 | 云南蓝钻生物科技股份有限公司 | A method of greatly improving content of the total saponins in radix notoginseng |
-
2020
- 2020-10-24 CN CN202011150134.3A patent/CN112205406A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104920214A (en) * | 2015-06-03 | 2015-09-23 | 中国科学院昆明植物研究所 | Method for improving yield of saponin in panax notoginseng tissue culture seedlings |
| CN105052581A (en) * | 2015-08-05 | 2015-11-18 | 广西壮族自治区药用植物园 | Method for improving ginsenoside Rg1 content in pseudo-ginseng |
| CN110122235A (en) * | 2019-05-24 | 2019-08-16 | 云南蓝钻生物科技股份有限公司 | A method of greatly improving content of the total saponins in radix notoginseng |
Non-Patent Citations (1)
| Title |
|---|
| 贾兵 等: ""建立UPLC-MS联合HPLC方法探究外源物质对三七叶皂苷含量和生长的影响"", 《云南农业大学学报(自然科学)》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931079A (en) * | 2021-02-24 | 2021-06-11 | 成都医学院 | Method for improving quality of selfheal medicinal materials by spraying exogenous hormones |
| CN113598003A (en) * | 2021-09-15 | 2021-11-05 | 昆明理工大学 | Method for improving synthesis of notoginsenoside and application |
| CN115500168A (en) * | 2022-10-20 | 2022-12-23 | 中南林业科技大学 | Method for regulating camellia oleifera gene by using methyl jasmonate |
| CN115500168B (en) * | 2022-10-20 | 2023-10-13 | 中南林业科技大学 | Method for regulating camellia oleifera genes by adopting methyl jasmonate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112205406A (en) | Method for rapidly increasing saponin content of panax notoginseng leaf | |
| Kim et al. | Lateral root development and saponin accumulation as affected by IBA or NAA in adventitious root cultures of Panax ginseng CA Meyer | |
| Dowom et al. | Enhanced phenolic acids production in regenerated shoot cultures of Salvia virgata Jacq. after elicitation with Ag+ ions, methyl jasmonate and yeast extract | |
| Xiao et al. | Accumulation characteristics and correlation analysis of five ginsenosides with different cultivation ages from different regions | |
| Han et al. | A high production of flavonoids and anthraquinones via adventitious root culture of Oplopanax elatus and evaluating antioxidant activity | |
| Kochan et al. | Effect of sugar concentration on ginsenoside biosynthesis in hairy root cultures of Panax quinquefolium cultivated in shake flasks and nutrient sprinkle bioreactor | |
| Krishnan et al. | Elicitation effect on the production of asiaticoside and asiatic acid in shoot, callus, and cell suspension culture of Centella asiatica | |
| CN102353735B (en) | Quality detection method for Tongmai Tangyanming capsule | |
| Niu et al. | Study on antibacterial effect of medlar and hawthorn compound extract in vitro | |
| Gupta et al. | Differential Expression of Thymoquinone and Its Localization in Different Parts of Nigella sativa L. | |
| CN110800745A (en) | Application of brassinolide in promoting growth and development of panax notoginseng | |
| CN113655130B (en) | HPLC detection method and application of ginsenoside Ro | |
| CN109696513A (en) | A method of identifying ginseng type and its growth year using ginsenoside component content and its ratio | |
| Wang et al. | Saponin accumulation in the seedling root of Panax notoginseng | |
| CN110827995B (en) | Characterization method of chromatography and mass spectrum fingerprint spectrum of secondary metabolites of nudiflower purple beautyberry medicinal material | |
| Mohan et al. | Gas chromatography-mass spectrometry (GC-MS) determination of phytoconstituents from ethanolic and aqua-ethanolic root extracts of Uraria picta Desv.(Fabaceae) | |
| CN101780130A (en) | Industrial extraction method of bo-chrysanthemum | |
| Zhang et al. | The content variation of four active components in Amygdalus persica L. during different harvesting periods | |
| CN111388542A (en) | Method for extracting monoterpene glycoside active ingredient from non-medicinal part of radix paeoniae alba | |
| Hong-Yan et al. | Autotoxins screening from aqueous extracts of salvia miltiorrhiza Bge. based on spectrum-effect relationship between HPLC fingerprints and autotoxicity | |
| Tatia et al. | Phytochemical and antiproliferative potential of Hedera Helix extract fraction | |
| Li et al. | Quantification and Discrimination of in Vitro Regeneration Swertia nervosa at Different Growth Periods using the UPLC/UV Coupled with Chemometric Method | |
| Liu et al. | Study on the process of ultrasonic extraction of total saponins from Panax notoginseng peduncles. | |
| Wang et al. | Gradually scale-up culture in a bioreactor promotes radical scavenging activity of Panax ginseng (CA Meyer) adventitious roots on 1, 1-diphenyl-2-picrylhydrazyl | |
| CN108283680A (en) | The direct oral traditional Chinese medicine medicine materical crude slice of dragon blood powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20210112 |