CN107318433A - The method for improving ginsenoside Re's content in cultivation field seven - Google Patents

The method for improving ginsenoside Re's content in cultivation field seven Download PDF

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CN107318433A
CN107318433A CN201710628571.3A CN201710628571A CN107318433A CN 107318433 A CN107318433 A CN 107318433A CN 201710628571 A CN201710628571 A CN 201710628571A CN 107318433 A CN107318433 A CN 107318433A
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pseudo
ginseng
ginsenoside
root
cultivation
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周运德
韦荣昌
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周运德
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/20Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom three- or four-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16Heavy metals; Compounds thereof
    • A01N59/20Copper
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]

Abstract

The invention discloses a kind of method for improving ginsenoside Re's content in cultivation field seven, harvested first 2 10 days in pseudo-ginseng, alternately pseudo-ginseng plant is sprayed with methyl jasmonate solution and salicylic acid solution.The present invention in pseudo-ginseng by harvesting the preceding blade that pseudo-ginseng is alternately sprayed with methyl jasmonate solution and salicylic acid solution, to induce the high of ginsenoside Re's key enzyme gene in cultivation field seven to express, so that the content of quick raising ginsenoside Re in a short time.

Description

The method for improving ginsenoside Re's content in cultivation field seven
Technical field
The present invention relates to the planting of medicinal materials field.It is more particularly related to which a kind of improve ginseng soap in cultivation field seven The method of glycosides Re contents.
Background technology
Pseudo-ginseng [Panax notoginseng (Burk.) F.H.Chen], is Araliaceae Panax herbaceos perennial, It is the famous traditional Chinese medicine of China, is mainly used as medicine with root and rhizome, warm-natured, sweet, slight bitter, with dissipate stasis of blood hemostasis, detumescence ding-tong The effect of.Pseudo-ginseng is famous Yunnan Baiyao, Fufang Danshen Pian (dripping pill), ZhangZhou Pien Tze Huang, logical, the red medicine in northeast of thrombus (plug) etc. The primary raw material of Chinese patent drug.Ginsenoside Re is one of main active in pseudo-ginseng, and ginsenoside Re has good medicine Reason activity, is clinically mainly used in many diseases such as neural, immune, cardiovascular and cerebrovascular, and it has to estrogen active, resisted There is positive effect, current someone's report ginsenoside Re can in terms of arrhythmia cordis, Parkinsonian symptoms, resisting kinetic fatigue Can be inhibited to the disease of some Tumor Differentiations or some tumours etc..Therefore, ginsenoside Re in pseudo-ginseng is improved Content is significant.Though the modes such as traditional at present cultivation, breeding can improve the yield of pseudo-ginseng to a certain extent, plant Train ginsenoside Re's content in pseudo-ginseng low, and traditional breeding method cycle length, difficulty are big, it is difficult to rapidly improve people in cultivation field seven Join saponin(e Re content.Prior art does not improve the report of ginsenoside Re's content in cultivation field seven.
The content of the invention
It is an object of the invention to solve the problem of ginsenoside Re's content is low in cultivation field seven, and provide behind at least By the advantage of explanation.
Ginseng soap in cultivation field seven is improved there is provided one kind according to object of the present invention and further advantage in order to realize The method of glycosides Re contents, it is characterised in that 2-10 days before pseudo-ginseng harvesting, is replaced with methyl jasmonate solution and salicylic acid solution Spray pseudo-ginseng plant.
Preferably, the concentration of the methyl jasmonate solution and salicylic acid solution be respectively 250-500 μm of ol/L and 200-400μmol/L。
Preferably, spraying method is:It is early, middle and late to be handed over the methyl jasmonate solution and the salicylic acid solution The blade surface 1 time of pseudo-ginseng plant is applied in tubing flow displacement, and the methyl jasmonate solution and the salicylic acid solution are sprayed every time to leaf Untill face is dripped.
Preferably, it is additionally included in before the transplanting of pseudo-ginseng seed bar, seed bar is handled with uv b radiation, the uv b radiation dosage is 3-5kj.m-2.d-1, processing time is 3-5d.
Preferably, the pseudo-ginseng plant is 3 years raw pseudo-ginseng plant.
Preferably, in pseudo-ginseng seed bar after uv b radiation, then it is 100-200 μm of ol/L that pseudo-ginseng seed bar is put in into concentration Soaked in nickel sulfate solution, after immersion 15-20min, the nickel sulfate solution immersed with pseudo-ginseng seed bar is put in into electric-field intensity is 7-10min is handled in 200-400kv/m electric field, is then put in after shady place 3-5h and is transplanted in pseudo-ginseng planting site.
Preferably, the root of fresh soyabean is taken, is smashed to pieces, filtrate is filtered to take, the pseudo-ginseng before field planting is smeared with filtrate The root of seedling, then wraps up 8-15min by its root with preservative film, removes preservative film, pseudo-ginseng seedling is put in into shady place 15- 20min, then in the root smearing first-time filtrate of pseudo-ginseng seedling, continuation wraps up pseudo-ginseng seedling root 5-10min with preservative film, again Remove preservative film, pseudo-ginseng seedling is put in shady place 10-15min, smear the root of a pseudo-ginseng seedling with filtrate again afterwards, most The root of pseudo-ginseng seedling is wrapped with black cotton afterwards and is placed on shady place 15-20min, black cotton of finally dismantling is colonized.
Preferably, prepare pseudo-ginseng foliage spraying agent, the foliage spraying agent by photosynthetic induction element 0.1-0.5 parts by weight, Copper sulphate 5-10 parts by weight, salicylic acid 4-8 parts by weight, dicyclohexylcarbodiimide 2-6 parts by weight, spinach juice 8-12 parts by weight, Taxol 1-5 parts by weight are made, and by 300-500 times of the foliage-spray dilution agent, in the every month, the 3rd grown in pseudo-ginseng is used The blade face of foliage spraying agent sprinkling pseudo-ginseng after dilution is untill blade face is dripped.
The present invention at least includes following beneficial effect:
(1) present invention in pseudo-ginseng by harvesting the preceding leaf that pseudo-ginseng is alternately sprayed with methyl jasmonate solution and salicylic acid solution Piece, to induce ginsenoside Re's key enzyme in cultivation field seven (such as squalene synthase, squalene epoxidase, glucosyltransferase With cytochrome P 450 enzymes etc.) the high expression of gene, so that the quick content for improving ginsenoside Re in a short time;
(2) present invention carries out uv b radiation processing to pseudo-ginseng seed bar, can further induce ginsenoside Re's generation in root tuber Thank to the high expression of key gene, and then improve the content of ginsenoside Re in the plant of cultivation field seven;
(3) present invention soaks pseudo-ginseng seed bar with nickel sulfate, then carries out electric field treatment, so as to improve nickel element in pseudo-ginseng seed bar Content, nickel element plays an important role during the nitrogen metabolism of pseudo-ginseng plant, and increase nickel element can be to a certain extent Increase the accumulation of ginsenoside Re in pseudo-ginseng;Soybean root filtrate is smeared in pseudo-ginseng seedling root, rihizobium japonicum can be applied The root of pseudo-ginseng seedling is smeared, Rhizobium Inoculation is realized, so as to realize increasing production and improying productivity for pseudo-ginseng, and then improves ginsenoside Re's Content;In the foliage spray spray of pseudo-ginseng plant, the growth of pseudo-ginseng plant can be accelerated and then accelerate activity in pseudo-ginseng plant The accumulation of composition, such as accelerates the accumulation of ginsenoside Re;
(4) present invention is easy to operate, and cost is low, environmentally friendly, suitable for large-scale production, with stronger practicality and Promotional value.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to specific embodiment, the present invention is described in further detail, to make those skilled in the art's reference say Bright book word can be implemented according to this.
It should be noted that experimental method described in following embodiments, is conventional method unless otherwise specified, institute Reagent and material are stated, unless otherwise specified, is commercially obtained.
Embodiment 1:
Before the transplanting of pseudo-ginseng seed bar, seed bar is handled with uv b radiation, uv b radiation dosage is 5kj.m-2.d-1, processing time For 3d, pseudo-ginseng seed bar is transplanted in planting site after having handled.Harvested first 2 days in pseudo-ginseng, it is early, middle and late to use methyl jasmonate Solution and salicylic acid solution alternately spray the blade surface 1 time of pseudo-ginseng plant, and methyl jasmonate solution and salicylic acid are sprayed every time Solution is untill blade face is dripped, and the concentration of wherein methyl jasmonate solution and salicylic acid solution is respectively 500 μm of ol/L and 400 μm ol/L, pseudo-ginseng plant is 3 years raw pseudo-ginseng plant.
Embodiment 2:
Before the transplanting of pseudo-ginseng seed bar, seed bar is handled with uv b radiation, uv b radiation dosage is 3kj.m-2.d-1, processing time For 5d, pseudo-ginseng seed bar is transplanted in planting site after having handled.Harvested first 8 days in pseudo-ginseng, it is early, middle and late to use methyl jasmonate Solution and salicylic acid solution alternately spray the blade surface 1 time of pseudo-ginseng plant, and methyl jasmonate solution and salicylic acid are sprayed every time Solution is untill blade face is dripped, and the concentration of wherein methyl jasmonate solution and salicylic acid solution is respectively 420 μm of ol/L and 310 μm ol/L, pseudo-ginseng plant is 3 years raw pseudo-ginseng plant.
Embodiment 3:
Before the transplanting of pseudo-ginseng seed bar, seed bar is handled with uv b radiation, uv b radiation dosage is 4kj.m-2.d-1, processing time For 4d, pseudo-ginseng seed bar is transplanted in planting site after having handled.Harvested first 10 days in pseudo-ginseng, it is early, middle and late to use methyl jasmonate Solution and salicylic acid solution alternately spray the blade surface 1 time of pseudo-ginseng plant, and methyl jasmonate solution and salicylic acid are sprayed every time Solution is untill blade face is dripped, and the concentration of wherein methyl jasmonate solution and salicylic acid solution is respectively 250 μm of ol/L and 200 μm ol/L, pseudo-ginseng plant is 3 years raw pseudo-ginseng plant.
Embodiment 4:
Before the transplanting of pseudo-ginseng seed bar, seed bar is handled with uv b radiation, uv b radiation dosage is 3kj.m-2.d-1, during processing Between be 5d, in pseudo-ginseng seed bar after uv b radiation, then pseudo-ginseng seed bar is put in concentration to soak in 100 μm of ol/L nickel sulfate solutions After bubble, immersion 20min, the nickel sulfate solution immersed with pseudo-ginseng seed bar is put in the electric field that electric-field intensity is 400kv/m and handled Pseudo-ginseng seed bar, is transplanted in planting site by 7min after having handled.The germination of pseudo-ginseng seed bar produces seedling, and growth of seedling is to a certain extent When, before pseudo-ginseng Seeding planting, the root of fresh soyabean is taken, is smashed to pieces, filtrate is filtered to take, the field before field planting is smeared with filtrate The root of seven seedling, then wraps up 8min by its root with preservative film, removes preservative film, pseudo-ginseng seedling is put in into shady place 15min, then in the root smearing first-time filtrate of pseudo-ginseng seedling, continuation is wrapped up pseudo-ginseng seedling root 10min with preservative film, gone again Fall preservative film, pseudo-ginseng seedling is put in shady place 15min, smear the root of a pseudo-ginseng seedling with filtrate again afterwards, finally use Black cotton, which wraps the root of pseudo-ginseng seedling, is placed on shady place 15min, then black cotton of dismantling is colonized.By following parts by weight Number takes each raw material:Photosynthetic induction element 0.1 part, 8 parts of copper sulphate, 4 parts of salicylic acid, 3 parts of dicyclohexylcarbodiimide, 8 parts of spinach juice, 1 part of taxol, above-mentioned each raw material is carried out to be mixed to prepare foliage spraying agent, by 300 times of foliage-spray dilution agent, in pseudo-ginseng growth In every month, the 3rd dilution after foliage spraying agent sprinkling pseudo-ginseng blade face untill blade face is dripped.Before pseudo-ginseng harvests 8 days, the early, middle and late blade surface 1 time that pseudo-ginseng plant is alternately sprayed with methyl jasmonate solution and salicylic acid solution, every time Methyl jasmonate solution and salicylic acid solution are sprayed untill blade face is dripped, wherein methyl jasmonate solution and salicylic acid solution Concentration be respectively 300 μm of ol/L and 280 μm of ol/L, pseudo-ginseng plant is 3 years raw pseudo-ginseng plant.
Embodiment 5:
Before the transplanting of pseudo-ginseng seed bar, seed bar is handled with uv b radiation, uv b radiation dosage is 3.5kj.m-2.d-1, during processing Between be 5d, in pseudo-ginseng seed bar after uv b radiation, then pseudo-ginseng seed bar is put in concentration to soak in 200 μm of ol/L nickel sulfate solutions After bubble, immersion 15min, the nickel sulfate solution immersed with pseudo-ginseng seed bar is put in the electric field that electric-field intensity is 200kv/m and handled Pseudo-ginseng seed bar, is transplanted in planting site by 10min after having handled.The germination of pseudo-ginseng seed bar produces seedling, growth of seedling to certain journey When spending, before pseudo-ginseng Seeding planting, the root of fresh soyabean is taken, is smashed to pieces, filtrate is filtered to take, smeared with filtrate before field planting The root of pseudo-ginseng seedling, then wraps up 15min by its root with preservative film, removes preservative film, pseudo-ginseng seedling is put in into shady place 20min, then in the root smearing first-time filtrate of pseudo-ginseng seedling, continuation is wrapped up pseudo-ginseng seedling root 5min with preservative film, gone again Fall preservative film, pseudo-ginseng seedling is put in shady place 10min, smear the root of a pseudo-ginseng seedling with filtrate again afterwards, finally use Black cotton, which wraps the root of pseudo-ginseng seedling, is placed on shady place 20min, then black cotton of dismantling is colonized.By following parts by weight Number takes each raw material:0.5 part of photosynthetic induction element, 5 parts of copper sulphate, 6 parts of salicylic acid, 6 parts of dicyclohexylcarbodiimide, spinach juice 10 Part, 3 parts of taxol, above-mentioned each raw material is carried out to be mixed to prepare foliage spraying agent, by 400 times of foliage-spray dilution agent, in pseudo-ginseng The blade face of foliage spraying agent sprinkling pseudo-ginseng after in every month, the 3rd dilution of growth is untill blade face is dripped.Adopted in pseudo-ginseng Receive preceding 10 days, it is early, middle and late to replace the blade surface 1 time for spraying pseudo-ginseng plant with methyl jasmonate solution and salicylic acid solution, Methyl jasmonate solution and salicylic acid solution are sprayed untill blade face is dripped every time, wherein methyl jasmonate solution and salicylic acid The concentration of solution is respectively 250 μm of ol/L and 400 μm of ol/L, and pseudo-ginseng plant is 3 years raw pseudo-ginseng plant.
Embodiment 6:
Before the transplanting of pseudo-ginseng seed bar, seed bar is handled with uv b radiation, uv b radiation dosage is 4.5kj.m-2.d-1, during processing Between be 4d, in pseudo-ginseng seed bar after uv b radiation, then pseudo-ginseng seed bar is put in concentration to soak in 180 μm of ol/L nickel sulfate solutions After bubble, immersion 18min, the nickel sulfate solution immersed with pseudo-ginseng seed bar is put in the electric field that electric-field intensity is 320kv/m and handled Pseudo-ginseng seed bar, is transplanted in planting site by 8min after having handled.The germination of pseudo-ginseng seed bar produces seedling, and growth of seedling is to a certain extent When, before pseudo-ginseng Seeding planting, the root of fresh soyabean is taken, is smashed to pieces, filtrate is filtered to take, the field before field planting is smeared with filtrate The root of seven seedling, then wraps up 12min by its root with preservative film, removes preservative film, pseudo-ginseng seedling is put in into shady place 18min, then in the root smearing first-time filtrate of pseudo-ginseng seedling, continuation is wrapped up pseudo-ginseng seedling root 7min with preservative film, gone again Fall preservative film, pseudo-ginseng seedling is put in shady place 13min, smear the root of a pseudo-ginseng seedling with filtrate again afterwards, finally use Black cotton, which wraps the root of pseudo-ginseng seedling, is placed on shady place 14min, then black cotton of dismantling is colonized.By following parts by weight Number takes each raw material:0.4 part of photosynthetic induction element, 10 parts of copper sulphate, 8 parts of salicylic acid, 2 parts of dicyclohexylcarbodiimide, spinach juice 12 Part, 5 parts of taxol, above-mentioned each raw material is carried out to be mixed to prepare foliage spraying agent, by 500 times of foliage-spray dilution agent, in pseudo-ginseng The blade face of foliage spraying agent sprinkling pseudo-ginseng after in every month, the 3rd dilution of growth is untill blade face is dripped.Adopted in pseudo-ginseng Receive preceding 5 days, it is early, middle and late to replace the blade surface 1 time for spraying pseudo-ginseng plant with methyl jasmonate solution and salicylic acid solution, Methyl jasmonate solution and salicylic acid solution are sprayed untill blade face is dripped every time, wherein methyl jasmonate solution and salicylic acid The concentration of solution is respectively 480 μm of ol/L and 400 μm of ol/L, and pseudo-ginseng plant is 3 years raw pseudo-ginseng plant.
The pseudo-ginseng root that embodiment 3, embodiment 6 are harvested and the pseudo-ginseng root of the method cultivation of routine take 6 groups respectively, Every group of 500g, according to《Chinese Pharmacopoeia》First " pseudo-ginseng " medicinal material【Assay】Method under, each group people is extracted with methanol Join saponin(e Re, then high effective liquid chromatography for measuring each group ginsenoside Re's content averages, obtained experimental result such as table Shown in 1:
Table 1
Experimental group Embodiment 3 Embodiment 6 Conventional method
Ginsenoside Re's content 0.40% 0.42% 0.33%
As can be seen from Table 1, the ginsenoside Re for the pseudo-ginseng root that embodiment 3 and embodiment 6 are cultivated is more than conventional method The pseudo-ginseng of cultivation, has been respectively increased 21.2% and 27.3%, therefore improves the economic value of pseudo-ginseng, and then improves peasant's Income and the enthusiasm of plantation.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited In specific details and shown here as the embodiment with description.

Claims (8)

1. a kind of method for improving ginsenoside Re's content in cultivation field seven, it is characterised in that 2-10 days before pseudo-ginseng harvesting, use Methyl jasmonate solution and salicylic acid solution alternately spray pseudo-ginseng plant.
2. the method for ginsenoside Re's content in cultivation field seven is improved as claimed in claim 1, it is characterised in that the jasmine Sour methyl ester solution and the concentration of salicylic acid solution are respectively 250-500 μm of ol/L and 200-400 μm of ol/L.
3. the method for ginsenoside Re's content in cultivation field seven is improved as claimed in claim 1, it is characterised in that spraying method For:The early, middle and late blade surface 1 that pseudo-ginseng plant is alternately sprayed with the methyl jasmonate solution and the salicylic acid solution It is secondary, the methyl jasmonate solution and the salicylic acid solution are sprayed every time untill blade face is dripped.
4. the method for ginsenoside Re's content in cultivation field seven is improved as claimed in claim 1, it is characterised in that be additionally included in Before pseudo-ginseng seed bar is transplanted, seed bar is handled with uv b radiation, the uv b radiation dosage is 3-5kj.m-2.d-1, processing time is 3- 5d。
5. the method for ginsenoside Re's content in cultivation field seven is improved as claimed in claim 1, it is characterised in that the pseudo-ginseng Plant is 3 years raw pseudo-ginseng plant.
6. the method for ginsenoside Re's content in cultivation field seven is improved as claimed in claim 4, it is characterised in that in pseudo-ginseng seed Bar is put in concentration to be soaked in 100-200 μm of ol/L nickel sulfate solution after uv b radiation, then by pseudo-ginseng seed bar, soaks 15- After 20min, the nickel sulfate solution immersed with pseudo-ginseng seed bar is put in the electric field that electric-field intensity is 200-400kv/m and handles 7- 10min, is then put in after shady place 3-5h and is transplanted in pseudo-ginseng planting site.
7. the method for ginsenoside Re's content in cultivation field seven is improved as claimed in claim 1, it is characterised in that taken fresh big The root of beans, is smashed to pieces, filters to take filtrate, and the root of the pseudo-ginseng seedling before field planting is smeared with filtrate, then will with preservative film 8-15min is wrapped up in its root, removes preservative film, pseudo-ginseng seedling is put in into shady place 15-20min, then in the root of pseudo-ginseng seedling First-time filtrate is smeared, continuation wraps up pseudo-ginseng seedling root 5-10min with preservative film, removes preservative film again, pseudo-ginseng seedling is put In shady place 10-15min, the root of a pseudo-ginseng seedling is smeared with filtrate again afterwards, finally with black cotton by pseudo-ginseng seedling Root, which is wrapped, is placed on shady place 15-20min, and black cotton of finally dismantling is colonized.
8. the method for ginsenoside Re's content in cultivation field seven is improved as claimed in claim 1, it is characterised in that prepare pseudo-ginseng Foliage spraying agent, the foliage spraying agent is by photosynthetic induction element 0.1-0.5 parts by weight, copper sulphate 5-10 parts by weight, salicylic acid 4-8 Parts by weight, dicyclohexylcarbodiimide 2-6 parts by weight, spinach juice 8-12 parts by weight, taxol 1-5 parts by weight are made, will be described 300-500 times of foliage-spray dilution agent, the foliage spraying agent after in every month, the 3rd dilution that pseudo-ginseng grows sprays pseudo-ginseng Blade face untill blade face is dripped.
CN201710628571.3A 2017-07-28 2017-07-28 The method for improving ginsenoside Re's content in cultivation field seven Pending CN107318433A (en)

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Application publication date: 20171107