CN110121563A - 在边合成边测序中作为成像试剂的光保护混合物 - Google Patents

在边合成边测序中作为成像试剂的光保护混合物 Download PDF

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Publication number
CN110121563A
CN110121563A CN201780078941.9A CN201780078941A CN110121563A CN 110121563 A CN110121563 A CN 110121563A CN 201780078941 A CN201780078941 A CN 201780078941A CN 110121563 A CN110121563 A CN 110121563A
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China
Prior art keywords
buffer
imaging
reagent
carnitine
photoprotective
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Pending
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CN201780078941.9A
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English (en)
Chinese (zh)
Inventor
L·安德鲁齐
M·佩尔博
D·赫夫罗尼
M·古普塔
A·里克
T·佩尔蒂埃
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Qi Gang Science Co Ltd
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Qi Gang Science Co Ltd
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Publication of CN110121563A publication Critical patent/CN110121563A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C215/00—Compounds containing amino and hydroxy groups bound to the same carbon skeleton
    • C07C215/02—Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C215/22—Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being unsaturated
    • C07C215/28—Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being unsaturated and containing six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
    • C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
    • C07D311/66—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached in position 2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/07—Optical isomers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN201780078941.9A 2016-11-09 2017-10-31 在边合成边测序中作为成像试剂的光保护混合物 Pending CN110121563A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662419702P 2016-11-09 2016-11-09
US62/419,702 2016-11-09
PCT/US2017/059318 WO2018089231A1 (en) 2016-11-09 2017-10-31 Photoprotective mixtures as imaging reagents in sequencing-by-synthesis

Publications (1)

Publication Number Publication Date
CN110121563A true CN110121563A (zh) 2019-08-13

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CN201780078941.9A Pending CN110121563A (zh) 2016-11-09 2017-10-31 在边合成边测序中作为成像试剂的光保护混合物

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Country Link
US (2) US11162129B2 (https=)
EP (1) EP3538093B1 (https=)
JP (1) JP7075937B2 (https=)
CN (1) CN110121563A (https=)
WO (1) WO2018089231A1 (https=)

Cited By (6)

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CN113795595A (zh) * 2020-04-20 2021-12-14 深圳华大智造科技股份有限公司 Dna成像缓冲液中的dna保护试剂
CN115078315A (zh) * 2021-03-12 2022-09-20 郑州思昆生物工程有限公司 抑制荧光强度降低的成像试剂、试剂盒
CN115074425A (zh) * 2021-03-12 2022-09-20 郑州思昆生物工程有限公司 基于荧光的多循环核酸测序反应用成像试剂、试剂盒
WO2023060527A1 (zh) * 2021-10-11 2023-04-20 深圳华大智造科技股份有限公司 皂苷类化合物在核酸测序中的用途
CN116926178A (zh) * 2022-04-02 2023-10-24 深圳市真迈生物科技有限公司 荧光检测试剂及其制备方法及应用
WO2024050820A1 (zh) * 2022-09-09 2024-03-14 深圳华大智造科技股份有限公司 抗氧化剂组合物及其在核酸检测中的用途

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WO2017193044A1 (en) * 2016-05-06 2017-11-09 Counsyl, Inc. Noninvasive prenatal diagnostic
KR102709865B1 (ko) 2017-05-05 2024-09-26 퀀텀-에스아이 인코포레이티드 생물학적 반응에 있어서 개질된 표면 반응성 및 오손방지 특성을 갖는 기재
SG11202012746XA (en) * 2018-12-17 2021-01-28 Illumina Cambridge Ltd Compositions for use in polyunucleotide sequencing
US20220098658A1 (en) * 2020-09-21 2022-03-31 Quantum-Si Incorporated Methods to minimize photodamage during nucleic acid and peptide sequencing
CA3196800A1 (en) * 2020-10-30 2022-05-05 Element Biosciences, Inc. Reagents for massively parallel nucleic acid sequencing
CN115917006A (zh) * 2021-09-07 2023-04-04 深圳华大智造科技股份有限公司 一种用于分析靶多核苷酸的序列的方法
CA3230995A1 (en) * 2021-09-07 2023-03-16 Meihua Gong Method for analyzing sequence of target polynucleotide
CN116334196A (zh) * 2022-08-12 2023-06-27 深圳赛陆医疗科技有限公司 一种优化的二代基因测序合成反应试剂

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113795595A (zh) * 2020-04-20 2021-12-14 深圳华大智造科技股份有限公司 Dna成像缓冲液中的dna保护试剂
CN113795595B (zh) * 2020-04-20 2023-02-28 深圳华大智造科技股份有限公司 Dna成像缓冲液中的dna保护试剂
CN115078315A (zh) * 2021-03-12 2022-09-20 郑州思昆生物工程有限公司 抑制荧光强度降低的成像试剂、试剂盒
CN115074425A (zh) * 2021-03-12 2022-09-20 郑州思昆生物工程有限公司 基于荧光的多循环核酸测序反应用成像试剂、试剂盒
CN115078315B (zh) * 2021-03-12 2025-12-26 郑州思昆生物工程有限公司 抑制荧光强度降低的成像试剂、试剂盒
CN115074425B (zh) * 2021-03-12 2026-04-21 郑州思昆生物工程有限公司 基于荧光的多循环核酸测序反应用成像试剂、试剂盒
WO2023060527A1 (zh) * 2021-10-11 2023-04-20 深圳华大智造科技股份有限公司 皂苷类化合物在核酸测序中的用途
CN116926178A (zh) * 2022-04-02 2023-10-24 深圳市真迈生物科技有限公司 荧光检测试剂及其制备方法及应用
CN116926178B (zh) * 2022-04-02 2025-10-24 深圳市真迈生物科技有限公司 荧光检测试剂及其制备方法及应用
WO2024050820A1 (zh) * 2022-09-09 2024-03-14 深圳华大智造科技股份有限公司 抗氧化剂组合物及其在核酸检测中的用途

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JP2020511955A (ja) 2020-04-23
EP3538093A1 (en) 2019-09-18
WO2018089231A1 (en) 2018-05-17
US11162129B2 (en) 2021-11-02
US20180127809A1 (en) 2018-05-10
EP3538093B1 (en) 2023-10-25
US20210403985A1 (en) 2021-12-30
JP7075937B2 (ja) 2022-05-26
EP3538093A4 (en) 2020-05-27

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