CN110090170A

CN110090170A - Biological polyoses and its application with hair growing anticreep

Info

Publication number: CN110090170A
Application number: CN201910497983.7A
Authority: CN
Inventors: 郭宏亮; 叶榛; 吴佳迪; 庄秀园; 林檬; 王轩; 朱勤健
Original assignee: Zhejiang Lien Biotechnology Co Ltd
Current assignee: Zhejiang Lien Biotechnology Co Ltd
Priority date: 2018-06-26
Filing date: 2019-06-10
Publication date: 2019-08-06
Anticipated expiration: 2039-06-10
Also published as: CN110090170B; WO2020001314A1

Abstract

The present invention relates to the biological polyoses with hair growing anticreep and its applications.Specifically, the beta glucan in the present invention especially has natural soluble, high molecule mass, highly viscous beta glucan (such as schizophyllum commune beta glucan), can effectively promote hair tonic and/or prevent hair loss.Experiment shows that beta glucan can be obviously promoted the hair follicle of stand-down and enter growth period, its long-time is made to be in growth period, promotes hair regeneration and hair growth, prevents hair loss, consolidates root of hair, and has and remove and pre- anti-dandruff and antipruritic effect.

Description

Biological polyoses and its application with hair growing anticreep

Technical field

The present invention relates to biotechnologys and field of pharmaceutical preparations, more particularly to the biological polyoses with hair growing anticreep And its application.

Background technique

The growth of hair is the process of a cyclic periodicity, mainly passes through growth period (anagen stage), catagen (catagen stage), 3 processes of stand-down (telogen stage).The growth of hair is not only by age, gender, position Influence, also influenced by body hormonal readiness, disease and extraneous factor etc..

Hair has the function of defence, protection and increases information interchange and beauty.Hair is generated by hair follicle, and hair follicle is a kind of Complicated skin accessory organ persistently experiencings the recurring cyclically growth of growth period, catagen and stand-down.Hair follicle week Phase once lacks of proper care, it will influences hair follicle normal growth so as to cause the generation of hair loss disorders.With social development, people are to having concurrently It protects and increasingly payes attention to the hair of beauty function, but rush hair growth drug clinically curative for effect is still very deficient.Mesh Before, only the oral drugs Finasteride and external drug minoxidil of U.S. FDA approval have curative effect relatively certainly.To promotion hair Capsule regeneration, the research of hair growth drug have been increasingly becoming the research hotspot and difficult point of dermatology.

Therefore, this field needs to develop a kind of product that can be effectively facilitated hair tonic, prevent hair loss.

Summary of the invention

The object of the present invention is to provide a kind of drugs that can be effectively facilitated hair tonic, prevent hair loss.

It is a further object of the present invention to provide a kind of biological polyoses with hair tonic and hair losing preventing and its applications in hair-care articles.

The first aspect of the present invention provides a kind of purposes of beta glucan, promotes hair tonic and/or anti-anti-avulsion for (a) Hair, or preparation or composition that (b) preparation promotes hair tonic and/or prevents hair loss.

In another preferred example, the beta glucan is callose.

In another preferred example, the beta glucan is β -1,3- glucan, it is preferable that there is β -1, the β -1 of 6- branch, 3- glucan.

In another preferred example, the structure of the beta glucan is shown in formula I,

Wherein, l is >=0 integer, preferably 0-50, preferably 0-10, more preferably 0-3, more preferably 1-2, more preferably 1；m It is more preferably 0-1 for >=0 integer, preferably 0-19, preferably 0-4, is more preferably 0；The integer that n is >=3, preferably Ground is 30-60000, more preferably 100-10000.

In another preferred example, l is the integer of 0-50, preferably 0-10, more preferably 0-3, more preferably 1-2, more preferably 1；m It is more preferably 0-1 for >=0 integer, preferably 0-19, preferably 0-4, is more preferably 0；The integer that n is >=3, preferably Ground is 30-60000, more preferably 100-10000.

In another preferred example, the l is selected from 0,1,2,3,4,5,6,7,8,9 or 10.

In another preferred example, the m is selected from 0,1,2,3,4,5,6,7,8,9 or 10.

In another preferred example, the degree of branching (DB) of the beta glucan is 0.02-0.8, preferably 0.1-0.5, preferably Ground 0.25-0.4, more preferably, 0.2-0.4.

In another preferred example, the beta glucan includes the beta glucan with three screw stereo structures.

In another preferred example, the content of the beta glucan of the three screw stereos structure is 80%, 90%, 95%, is pressed The total moles meter of beta glucan.

In another preferred example, β -1 of the beta glucan, 3- main chain are the main body of three screw stereo structures.

In another preferred example, β -1 of the beta glucan, 6- branch are located at the outside of three screw stereo structures.

In another preferred example, molecular weight >=2kD of the beta glucan, preferably 2kD-40000kD, more preferably 20kD-20000kD。

In another preferred example, the molecular weight of the beta glucan can be 5kD-35000kD；10kD-30000kD； 50kD-25000kD；100kD-20000kD；200kD-18000kD；400kD-16000kD；500kD-14000kD；1000kD- 12000kD；2000kD-4000kD；3000kD-5000kD；4000kD-6000kD；5000kD-7000kD；6000kD- 8000kD；7000kD-9000kD；Or 8000kD-10000kD.

In another preferred example, the beta glucan is selected from the group: schizophyllum commune beta glucan, β-lentinan, small nut Bacterium beta glucan, grifola frondosa beta-glucose, polysaccharide of picking up the ears, mushroom beta glucan, yeast beta-dextran, avenabeta glucosan or A combination thereof.

In another preferred example, the beta glucan is schizophyllum commune beta glucan.

In another preferred example, the β-lentinan is every 5 β -1, and the main chain of 3- has 2 β -1, point of 6- Branch, and the beta glucan of 1 glucose residue of each branch.

In another preferred example, purity >=70% of the beta glucan, preferably >=90%, more preferably >=95%, more Goodly >=99%.

In another preferred example, the beta glucan is with good stability.

In another preferred example, the beta glucan is in solid-state form or liquid form, such as beta glucan solid particle or Powder or beta glucan aqueous solution.

In another preferred example, partial size≤20mm of the beta glucan particle or powder, preferably 0.001-10mm, more Good ground 0.01-5mm, more preferably 0.1-2mm.

In another preferred example, the beta glucan is completely water-soluble beta glucan.

In another preferred example, the beta glucan (particle or powder) has good water-soluble and/or natural soluble Property.

In another preferred example, solubility of the beta glucan (particle or powder) in 25 DEG C of water (100g) >= 0.0001g, preferably 0.01-50g, more preferably 0.1-10g.

In another preferred example, solubility of the beta glucan (particle or powder) in 25 DEG C of water (100g) can be 0.1-100g；0.2-90g；0.5-80g；1-50g；Alternatively, the solubility can be 0.1-0.3g；0.2-0.4g；0.3- 0.5g；0.4-0.6g；0.5-0.7g；0.6-0.8g；0.7-0.9g；0.8-1g；Or 1-3g；2-4g；3-5g；4-6g；5-7g； 6-8g；7-9g；Or 8-10g.

In another preferred example, the beta glucan solution is the solution of beta glucan in water, i.e. beta glucan is water-soluble Liquid.

In another preferred example, beta glucan (water) solution viscosity is big；Preferably, the β-that mass concentration is 0.5% Glucan aqueous solution (at 25 DEG C) viscosity >=40mPas, more preferably 100-10000mPas, more preferably 500-2000mPa s。

In another preferred example, beta glucan aqueous solution (25 DEG C) viscosity that the mass concentration is 0.5% can be 50- 10000mPa·s；100-9000mPa·s；200-8000mPa·s；300-7000mPa·s；400-6000mPa·s；450- 5000mPa·s；500-5000mPa·s；550-4000mPa·s；600-3000mPa·s；650-2000mPa·s；Or 700-1500mPa·s。

In another preferred example, the aqueous solution for the beta glucan that the mass concentration is 1% has high clarity or high saturating Light rate, light transmittance >=50% for the beta glucan aqueous solution that the mass concentration is 1%, preferably >=80%, preferably >= 85%, more preferably >=95%；

In another preferred example, the beta glucan solution is with good stability.

In another preferred example, described to promote hair tonic and/or prevent hair loss including promoting the hair follicle of stand-down to enter growth Phase.

In another preferred example, it is described promote hair tonic and/or prevent hair loss include: promote hair papilla cell proliferation and/ Or activation, delay hair follicle cell regression, or combinations thereof.

In another preferred example, the promotion hair tonic includes promoting hair regeneration and/or hair growth.

In another preferred example, the alopecia includes genetic determination and/or acquisition form alopecia.

In another preferred example, the genetic determination or obtain form alopecia include hair growth deficiency, alopecia areata, portion Divide alopecia, alopecia capitis totalis, trichotillomania or drug-induced alopecia.

In another preferred example, the alopecia includes alopecia seborrheica, alopecia of chemical origin.

In another preferred example, the alopecia includes alopecia cicatrisata and/or non-alopecia cicatrisata.

In another preferred example, the alopecia cicatrisata includes that lichen planopilaris, post menopausal alopecia, lupus are de- Hair, Brocq pseudopelade, scalp segmentation property cellulitis, or combinations thereof.

In another preferred example, the non-alopecia cicatrisata includes that androgenetic alopecia, alopecia areata, temples triangle are de- The loose syndrome of hair, trichotillomania, alopecia senilis, telogen effluvim, anagen hair, alopecia syphilitica, the edemous alopecia of rouge, Salt alopecia, sugar alopecia, cancer-related alopecia, or combinations thereof, preferably androgenetic alopecia and/or alopecia areata.

In another preferred example, the non-alopecia cicatrisata includes that androgenetic alopecia, alopecia areata, temples triangle are de- The loose syndrome of hair, trichotillomania, alopecia senilis, telogen effluvim, anagen hair, alopecia syphilitica, the edemous alopecia of rouge, Salt alopecia, sugar alopecia, or combinations thereof, preferably androgenetic alopecia and/or alopecia areata.

In another preferred example, described to prevent hair loss including preventing hair loss and/or consolidating root of hair.

In another preferred example, the beta glucan, preparation or composition are also used to maintenance hair scale piece, improve hair quality.

In another preferred example, the beta glucan, preparation or composition are also used to removing and/or pre- anti-dandruff, stop Itch, repair damaged scalp or hair follicle, and/or confrontation head infection.

In another preferred example, the beta glucan, preparation or composition are also used to prevent and/or treat scalp inflammation, Preferably epifolliculitis, seborrhea.

In another preferred example, the preparation or composition contain (a) beta glucan；And optional (b) pharmaceutically, change Cosmetic is upper or the acceptable carrier of weapon exercises or excipient.

In another preferred example, the preparation or composition contain 0.001-99wt%, preferably 0.002-30wt%, more The beta glucan of good ground 0.005-10wt%, more preferably 0.01-3wt%, more preferably 0.02-1wt%, by preparation or composition Total weight.

In another preferred example, mass concentration of the beta glucan in the preparation or composition is >=1 μ g/mL, It is specifically as follows 1 μ g/mL-200mg/mL perhaps 1 μ g/mL-10mg/mL perhaps 1 μ g/mL-5mg/mL or 1 μ g/mL- 3mg/mL。

In another preferred example, the dosage form of the composition or preparation be pharmaceutically, food or health care or daily use chemicals it is acceptable Dosage form, including solid dosage forms, semisolid dosage form or liquid dosage form, such as solution, gel, cream, lotion.

In another preferred example, the composition is pharmaceutical composition, cosmetic composition, food or health food combination Object, and combinations thereof.

In another preferred example, the composition is pharmaceutical composition or cosmetic composition.

In another preferred example, the composition is oral, external application and/or the combination of the two, preferably external application, more It goodly is external preparation for skin.

The second aspect of the present invention provides a kind of preparation for promoting hair tonic and/or preventing hair loss, the preparation packet Containing the beta glucan as active constituent.

In another preferred example, the preparation contains 0.001-99wt%, preferably 0.002-30wt%, more preferably The beta glucan of 0.005-10wt%, more preferably 0.01-3wt%, more preferably 0.02-1wt%, by the total weight of preparation.

In another preferred example, the preparation further includes the second active constituent, and second active constituent is different from β- The promotion hair tonic of glucan and/or the active constituent for preventing hair loss active constituent or treatment scalp inflammation.

In another preferred example, second active constituent from chemically synthesized active constituent, natural drug source and/ Or the active constituent of food source.

In another preferred example, second active constituent is hair tonic medicament and/or anti-inflammation drugs.

In another preferred example, the hair growing medical anti-alopecia includes chemically synthesized hair tonic medicament, natural hair restorer Object, the food with hair tonic, the active peptides with strengthen the hair hair tonic, or combinations thereof.

In another preferred example, the hair tonic medicament is chemically synthesized hair tonic medicament, natural hair tonic medicament and/or has The active peptides of strengthen the hair hair tonic effect.

In another preferred example, the chemically synthesized hair tonic medicament is selected from the group: Finasteride, Cimetidine, An Ti Shu Tong, Flutamide, minoxidil, or combinations thereof.

In another preferred example, the natural hair tonic medicament is selected from the group: the fruit of glossy privet, the fleece-flower root, cacumen biotae or its group It closes.

In another preferred example, the natural hair tonic medicament is selected from the group: the fruit of glossy privet, the fleece-flower root, or combinations thereof.

In another preferred example, the food with hair tonic is selected from the group: ginger, Semen sesami nigrum, or combinations thereof.

In another preferred example, the active peptides are selected from the group: vascular endothelial growth factor, aspartic acid-silk Propylhomoserin-serine repetition polypeptide, or combinations thereof.

In another preferred example, the anti-inflammation drugs are selected from the group: antibiotic, bacteriostatic agent, V-A acidic, peroxidating Benzoyl, plant sterilization antipathogenic composition, glucocorticoid, azelaic acid, niacinamide, sulphur, resorcinol, salicylic acid, or combinations thereof. Another kind of is Chinese medicine and its extract: such as Rhizoma Atractylodis Macrocephalae, the tuber of pinellia, radix bupleuri, dried orange peel, radix paeoniae rubra, rheum officinale, glutinous rehmannia, Poria cocos, Radix Glycyrrhizae, ramulus cinnamomi, Huang Company, radix scutellariae, centella, turmeric, honeysuckle, Fructus Forsythiae, tree peony root/skin, great burdock achene, loguat leaf, dandelion, ginseng, the root bark of white mulberry, mountain Medicine, raw hawthorn, dark plum, wild chrysanthemum, Semen Coicis Semen Coicis, stir-baked FRUCTUS GARDENIAE, the dried immature fruit of citron orange, caulis bambusae in taenian, or combinations thereof.

In another preferred example, the preparation further includes pharmaceutically, can connect in cosmetics, food and/or health food Carrier, excipient or the additive received.

It is in another preferred example, described that pharmaceutically, on cosmetics, food acceptable carrier, excipient or additive select From the following group: surfactant, emulsifier, thickener, preservative, antioxidant, or combinations thereof.

In another preferred example, the dosage form of the preparation is pharmaceutically or health care or the acceptable dosage form of daily use chemicals, preferably For oral preparation (such as oral solutions, capsule), external preparation or preparation capable of permeating skin (such as externally used solution agent, ointment, patch).

In another preferred example, the preparation is liquid, foam-like, spray form, powdery, gel or paste.

In another preferred example, the preparation is used for the warm-blooded animal body with hair follicle, preferably people, domestic animal, pet.

In another preferred example, the preparation is selected from the group: hair tonic and/or Anti-hair loss liquid medicine, hair tonic and/or anticreep dispensing Cream, hair tonic and/or Anti-hair loss drug or patch, shampoo, profit hair-cream or hair conditioner, hair jelly, mousse, paint, hair cream, hair film, Hair rinse, eyebrow regenerative agent, eyelashes regenerative agent or eyelashes nutritional agents, hair-care essence liquid, or combinations thereof.

In another preferred example, the preparation is used to prepare product selected from the group below: hair tonic and/or Anti-hair loss liquid medicine, life Hair and/or Anti-hair loss ointment, hair tonic and/or Anti-hair loss drug or patch, shampoo, profit hair-cream or hair conditioner, hair jelly, mousse, Paint, hair cream, hair film, hair rinse, eyebrow regenerative agent, eyelashes regenerative agent or eyelashes nutritional agents, hair-care essence liquid, or combinations thereof.

In another preferred example, the beta glucan is selected from the group: schizophyllum commune beta glucan, β-lentinan, small nut Bacterium beta glucan, grifola frondosa beta-glucose, polysaccharide of picking up the ears, mushroom beta glucan, yeast beta-dextran, avenabeta glucosan or A combination thereof, it is preferable that the beta glucan is schizophyllum commune beta glucan.

In another preferred example, the beta glucan in the preparation, which has, is selected from the group one or more features:

(1) purity >=70% of the beta glucan, preferably >=90%, more preferably >=95%, more preferably >=99%；

(2) beta glucan has good water-soluble, solubility and/or natural soluble；

(3) solubility >=0.0001g/100g water of the beta glucan (solid particle or powder) in 25 DEG C of water, compared with Good ground 0.01-50g/100g water, more preferably 0.1g-10g/100g water；

(4) aqueous solution of the beta glucan (solid particle or powder) has high clarity or high transparency；Preferably, Light transmittance >=50% for the beta glucan aqueous solution that the mass concentration is 1%, preferably >=80%, preferably >=85%, more Goodly >=95%；

(5) solution viscosity of the beta glucan (solid particle or powder) is big；Preferably, mass concentration 0.5% Beta glucan aqueous solution (at 25 DEG C) viscosity >=40mPas, more preferably 100-10000, more preferably 600-2000mPas；

(6) aqueous solution of the beta glucan is with good stability；And/or

(7) molecular weight >=2kD of the beta glucan, preferably 2kD-40000kD, more preferably 20kD-20000kD.

In another preferred example, pharmaceutically or on cosmeceutical acceptable carrier is water.

The third aspect of the present invention provides the purposes of preparation as described in respect of the second aspect of the invention, is used to prepare and is selected from The product of the following group: hair tonic and/or Anti-hair loss liquid medicine, hair tonic and/or Anti-hair loss ointment, hair tonic and/or Anti-hair loss drug or patch, Shampoo, profit hair-cream or hair conditioner, hair jelly, mousse, paint, hair cream, hair film, hair rinse, eyebrow regenerative agent, eyelashes regenerative agent or Eyelashes nutritional agents, hair-care essence liquid, or combinations thereof.

The fourth aspect of the present invention, provides a kind of composition product, and the composition product includes:

(1) first chamber, the first chamber contain (a) first active constituent, and first active constituent is β- Glucan；(b) pharmaceutically, on cosmeceutical, food and/or health food be upper or the acceptable carrier of weapon exercises or tax Shape agent；And

(2) second chamber, the second chamber are hair growing medical anti-alopecia or treatment head dermatitis disease drug.

(2) beta glucan has good water-soluble, solubility and/or natural soluble；

(6) aqueous solution of the beta glucan is with good stability；And/or

In another preferred example, pharmaceutically, on cosmeceutical or the upper acceptable carrier of food and/or health food For water.

In another preferred example, the hair growing medical anti-alopecia contains (a) second active constituent, second activity at It is divided into the hair growing anticreep active constituent different from beta glucan or treats the active constituent of scalp inflammation；(b) pharmacy On upper, cosmeceutical, food and/or health food are gone up or the acceptable carrier of weapon exercises or excipient.

In another preferred example, the treatment head dermatitis disease drug is selected from the group: antibiotic, bacteriostatic agent, vitamin A acid Class, benzoyl peroxide, plant sterilization antipathogenic composition, glucocorticoid, azelaic acid, niacinamide, sulphur, resorcinol, salicylic acid, Or combinations thereof.Another kind of is Chinese medicine and its extract: such as the Rhizoma Atractylodis Macrocephalae, tuber of pinellia, radix bupleuri, dried orange peel, radix paeoniae rubra, rheum officinale, glutinous rehmannia, Poria cocos, sweet Grass, ramulus cinnamomi, the coptis, radix scutellariae, centella, turmeric, honeysuckle, Fructus Forsythiae, tree peony root/skin, great burdock achene, loguat leaf, dandelion, people Ginseng, the root bark of white mulberry, Chinese yam, raw hawthorn, dark plum, wild chrysanthemum, Semen Coicis Semen Coicis, stir-baked FRUCTUS GARDENIAE, the dried immature fruit of citron orange, caulis bambusae in taenian, or combinations thereof.

In another preferred example, the first chamber and second chamber are independent or be combined into one.

In another preferred example, the composition product is for promoting hair tonic and/or preventing hair loss.

In another preferred example, the composition product is for treating, preventing and/or improving alopecia.

In another preferred example, the composition product be cosmetics, drug, food, or combinations thereof.

The fifth aspect of the present invention, provides a kind of hair care products, and the product includes: as active constituent Beta glucan.

In another preferred example, the hair care products are selected from the group: hair tonic and/or Anti-hair loss liquid medicine, hair tonic and/or Anti-hair loss ointment, hair tonic and/or Anti-hair loss drug or patch, shampoo, profit hair-cream or hair conditioner, hair jelly, mousse, paint, hair Cream, hair film, hair rinse, eyebrow regenerative agent, eyelashes regenerative agent or eyelashes nutritional agents, hair-care essence liquid, or combinations thereof.

The sixth aspect of the present invention provides a kind of method for promoting hair tonic and/or preventing hair loss, comprising steps of (a) gives Object application beta glucan, the preparation as described in respect of the second aspect of the invention, composition described in fourth aspect present invention needed Hair care products described in product or fifth aspect present invention.

In another preferred example, the object includes the warm-blooded animal body with hair follicle, preferably people, domestic animal, pet.

In another preferred example, the object suffers from genetic determination and/or acquisition form alopecia.

In another preferred example, the genetic determination or obtain the alopecia of form be alopecia areata, it is part alopecia, all de- Hair, trichotillomania or drug-induced alopecia.

It should be understood that above-mentioned each technical characteristic of the invention and having in below (eg embodiment) within the scope of the present invention It can be combined with each other between each technical characteristic of body description, to form a new or preferred technical solution.As space is limited, exist This no longer tires out one by one states.

Detailed description of the invention

Fig. 1 shows the Fourier transform infrared spectroscopy figure of the schizophyllum commune beta glucan prepared in embodiment 1.

Fig. 2 shows that schizophyllum commune beta glucan has the function of remarkably promoting hair growth.

Fig. 3 shows that schizophyllum commune beta glucan has the stack height for increasing AES foam, slows down antifoaming speed and increases bubble The effect of foam sophistication, wherein A is to increase foam stack height, and B is to slow down antifoaming speed, and C is to increase foam sophistication.

Fig. 4, which is shown, has better chip-removing capacity without silicone oil shampoo containing schizophyllum commune beta glucan, and extends Hair returns the oily time and improves hair quality.

Fig. 5 shows that schizophyllum commune beta glucan has the function of significantly repairing hair scale piece.

Fig. 6 shows that schizophyllum commune beta glucan has the function of remarkably promoting human hair growth.

Fig. 7 shows that schizophyllum commune beta glucan has the function of remarkably promoting human hair growth.

Fig. 8 shows that schizophyllum commune beta glucan has the function of remarkably promoting human hair growth.

Specific embodiment

The present inventor after extensive and in-depth study, for the first time it was unexpectedly observed that beta glucan, especially has and naturally may be used Dissolubility, high molecular weight, highly viscous beta glucan (such as schizophyllum commune beta glucan), can effectively promote hair tonic and/or It prevents hair loss.Experiment shows that beta glucan has the hair follicle for being obviously promoted stand-down to enter growth period, so that for a long time in growth Phase promotes hair regeneration and hair growth, removing and pre- anti-dandruff, antipruritic, reparation damaged scalp or hair follicle, and pre- anticreep It sends out, consolidate root of hair.Additionally it is possible to the fundamental characteristics of optimizing surface activating agent, improve bubble production volume, antifoaming speed be slow, foam more It is fine and smooth.On this basis, the present invention is completed.

Term explanation

Unless otherwise defined, otherwise whole technologies used herein and scientific term all have such as fields of the present invention The normally understood identical meanings of those of ordinary skill.

As used herein, in use, term " about " means that the value can be from enumerating in mentioning the numerical value specifically enumerated Value changes not more than 1%.For example, as used herein, statement " about 100 " include 99 and 101 and between whole values (for example, 99.1,99.2,99.3,99.4 etc.).

As used herein, term " containing " or " including (including) " can be open, semi-enclosed and enclosed.It changes Yan Zhi, the term also include " substantially by ... constitute " or " by ... constitute ".

As used herein, term " hair tonic ", " educating hair " are used interchangeably, and specifically include that hair regeneration and/or hair are raw It is long.

As used herein, term " preventing hair loss ", which refers to, prevents, inhibits, prevents and reduces partially or completely alopecia.

As used herein, term " promoting hair regeneration " is defined to include maintenance, induction, stimulation, promotion and restores hair Hair generates, lacks the extension in growth period in the growth or growth cycle of hair of hair.

As used herein, term " hair growth " is defined to include anticreep, the development for promoting hair and growth and milli Conversion of the hair to terminal hair.

As used herein, the beta glucan that term " completely water-soluble " refers to the solid-state form, can be completely dissolved in water As beta glucan aqueous solution, i.e., solubility >=0.0001g of beta glucan in 25 DEG C of 100g water, preferably 0.01-50g, More preferably 0.1g-10g.

As used herein, term " natural soluble " refers to that beta glucan of native state itself is possessed, complete in water The property of fully dissolved formation aqueous solution." beta glucan of native state " refers to and produces to obtain (such as life using natural method Object fermentation) beta glucan, the beta glucan by any chemical modification and without by any physics and/ Or its long-chain molecule is interrupted and reduces its molecular mass by chemical and/or biological method.In another preferred example, of the invention Beta glucan is the beta glucan of native state.

Alopecia

As used herein, term " alopecia " or " baldness " refer to hair growth deficiency and hair part or completely fall off, Including but not limited to androgenetic alopecia disease or male pattern baldness, alopecia toxica disease, alopecia areata, telogen effluvim, because in Baldness, drug-induced baldness, mechanicalness alopecia, skin disease caused by secretion abnormality, metabolic disorder or malnutrition are simultaneously Baldness, alopecia cicatrisata disease, congenital alopecia disease and the trichotillomania of hair.

Alopecia is the clinically most common skin diseases, different for its classification method, now most common It is that alopecia is divided into alopecia cicatrisata and non-alopecia cicatrisata two major classes.Wherein non-alopecia cicatrisata is reversible, and cicatricial is de- Hair is permanent.Alopecia cicatrisata is divided to primary and two kinds secondary again, and primary refers to the disease of hair follicle, and secondary is Refer to that hair follicle is not the main portions of inflammation.Non- alopecia cicatrisata include androgenetic alopecia, alopecia areata, the alopecia of temples triangle, The loose syndrome of trichotillomania, alopecia senilis, telogen effluvim, anagen hair, alopecia syphilitica, the edemous alopecia of rouge, food Salt alopecia, sugar alopecia, most commonly androgenetic alopecia and alopecia areata.Alopecia cicatrisata includes the flat tongue fur of hair Moss, post menopausal alopecia, lupus alopecia, Brocq pseudopelade, scalp segmentation property cellulitis.

Beta glucan

Beta glucan is a kind of natural polysaccharide, can find the beta glucan of suitable multiple types in the natural environment, usually It is present in the cell wall of the bacterium of Special Category, saccharomycete, fungi (ganoderma lucidum), also is present in the coating of higher plant seed In.There are mainly two types of the production methods of beta glucan, one is directly mentioning from the fructifications fungi such as the cereal such as oat or mushroom It takes；The second is processing is extracted via to fermentation liquid, by the liquid fermentation of fungi or bacterium to obtain beta glucan.

As used herein, " beta glucan of the present invention ", " biological polyoses of the present invention " are used interchangeably, and are primarily referred to as the present invention Beta glucan described in first aspect, the beta glucan are selected from the group: schizophyllum commune beta glucan, β-lentinan, small nut Bacterium beta glucan, grifola frondosa beta-glucose, polysaccharide of picking up the ears, mushroom beta glucan, yeast beta-dextran, avenabeta glucosan or A combination thereof；Preferably schizophyllum commune beta glucan.

As used herein, " schizophyllum commune beta glucan " refers to the beta glucan from schizophyllum commune.

In another preferred example, the structure of the beta glucan is shown in formula I.

In another preferred example, the beta glucan is with good stability.

In another preferred example, the beta glucan solution is with good stability.

In another preferred example, the beta glucan derives from higher plant or various bacteriums, fungi.

The embodiment of the present invention specifically by taking the tunning of schizophyllum commune as an example, but not limited to this.The hair follicle of health is scalp With the basis of hair healthy.Beta glucan of the present invention can not only promote hair tonic and prevent hair loss, but also scalp can also be helped to support Imperial extrinsic damage and infringement (such as fungi), effectively prevent the skin of head problem such as epifolliculitis, papule and seborrhea, promote Into scalp health.

In addition, beta glucan of the present invention have it is good wrapped, hair can be wrapped up, while effective water lock, protect Hair care silk simultaneously improves hair quality.It is used for shampoo, hair conditioner and hair film etc. and washes in shield product, has good conditioning function to hair Energy plays antistatic, increase hair soft degree, improves dry, wet cardability, and the effects of reduction hair entanglement.In addition, relaxing Delay antipruritic and antibacterial, antiviral etc., beta glucan of the present invention equally has remarkable efficacy, especially for no silicone oil shampoo Wash it is rear it is dry and astringent, the drawbacks such as be difficult to comb, there is splendid improvement.

Preparation or composition

The present invention provides a kind of preparation for promoting hair tonic and/or preventing hair loss, the preparation includes as activity The beta glucan of ingredient.

In another preferred example, the preparation contains (a) beta glucan；And optional (b) pharmaceutically, cosmeceutical Upper, food and/or health food are gone up or the acceptable carrier of weapon exercises or excipient.

In another preferred example, second active constituent, from chemically synthesized active constituent, natural drug and/or The active constituent of food source.

The present invention also provides a kind of composition product, the composition product includes:

In another preferred example, a effective amount of containing safe and effective amount or cosmeceutical in the preparation or composition product Active constituent (including the first active constituent and/or second active constituent).

In this expository writing, " safe and effective amount " is referred to: the amount of active constituent is enough to be obviously improved the state of an illness or symptom, and It is unlikely to generate serious side effect.

In this expository writing, term " cosmeceutical effective quantity " means preparation or the combination of sufficiently reaching aforementioned present invention The amount of the effect of object.

The carrier pharmaceutically or on cosmeceutical received is usually utilized in preparation or composition, and it includes lactose (lactose), glucose (dextrose), sucrose (sucrose), D-sorbite (sorbitol), mannitol (mannitol), Starch, Arabic gum (acaciagum), calcium phosphate (calciumphosphate), alginates (alginate), gelatin (gelatin), calcium silicates (calciumsilicate), microcrystalline cellulose (microcrystallinecellulose), poly- second Alkene pyrrolidone (polyvinylpyrrolidone), cellulose (cellulose), water, syrup (syrup), methylcellulose (methylcellulose), methyl hydroxybenzoate (methylhydroxybenzoate), nipasol (propylhydroxybenzoate), talcum, magnesium stearate (magnesiumstearate) and mineral oil (mineraloil) Deng however, it is not limited to this.

As the carrier for preparation or composition of the invention, any conventional carrier, example packet can be used Include pure water, mineral water, ethyl alcohol, glycerol, squalene, 1,3- propylene glycol, 1,3 butylene glycol, castor oil, camellia oil and liquid petrolatum.

Preparation or composition of the invention can with transdermal administration, for this purpose, can be formulated as washing lotion, ointment, emulsifiable paste, Patch, spray, tablet, pill, powder, sachet, elixir, suspension, lotion, solution.

Preparation or composition of the invention person of an ordinary skill in the technical field can be easy implementation according to the present invention Method, it is formulation using carrier and/or the excipient progress pharmaceutically or on cosmeceutical received, it thus prepares as unit appearance It measures in form, or loading to large-rolume container and prepares.Specifically, dosage form can be solution in oiliness or aqueous medium, Suspension or emulsion form, or it is also possible to extract, pulvis, granule, tablet or capsule or gel (for example, water Gel) form also may include dispersing agent or stabilizer.Preparation or composition of the invention also can be with usual institute in this field Preparation any dosage form be made, for example, can with solution, suspension, emulsion, paste, gel, creams, lotion, powder, soap, contain There are the dosage forms such as surfactant cleaning agent, oil and spray, however, it is not limited to this.

It is available as carrier components in the case that the dosage form of invention formulation or composition is paste, creams or gel Animal oil, vegetable oil, wax, paraffin, starch, traganth, cellulose derivative, polyethylene glycol, silicone, bentonite, titanium dioxide Silicon, talcum or zinc oxide etc..

In the case that dosage form of the invention is powder or spray, as carrier components, using lactose, talcum, titanium dioxide Silicon, aluminium hydroxide, calcium silicates or Silon also may include chlorofluorocarbons, propane/butane or two especially in the case where spray The propellants such as methyl ether.

In the case that dosage form of the invention is solution or emulsion, as carrier components, solvent, solubilizer or milkiness are utilized Agent, such as have water, ethyl alcohol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, Ergol, propylene glycol, 1,3- butyl second The aliphatic ester of glycol oil, glycerin fatty ester, polyethylene glycol or sorbitan.

In the case that dosage form of the invention is suspension, as carrier components, using liquids such as water, ethyl alcohol or propylene glycol The suspending agents such as diluent, ethoxylated isostearyl alcohols, polyoxyethylene sorbitan ester and polyoxyethylene sorbitol acid anhydride ester, crystallite are fine Tie up element, methyl aluminium hydroxide, bentonite, agar or traganth etc..

Preparation of the invention other than mentioned component, can also comprising commonly used in drug or cosmetic composition at Point, such as lubricant, wetting agent, sweetener, flavouring agent, emulsifier, surfactant, suspending agent, thickener, preservative, antioxygen The auxiliary agent of the routine such as agent, antistaling agent, stabilizer, solubilizer, vitamin, pigment and fragrance.

Specifically, preparation of the invention or composition can be used and directly smearing or spraying.This hair can be applied The hair of bright composition includes root of hair and hair follicle, and including hair, eyebrow and eyelashes, the chin hair, beard, whiskers, armpit on head With any region of root of hair and hair follicle on hair and body.Therefore, the present invention may be used as hair lotion, hair-cream, hair cream, hair jelly, Mousse, hair gel, hair conditioner, shampoo, hair rinse, treatment wax, hair nursing agent, eyebrow regenerative agent, eyelashes regenerative agent Or eyelashes nutritional agents or shampoo for pets and hair dye.

The method for promoting hair tonic and/or preventing hair loss

The present invention also provides a kind of promotion hair tonic and/or the method that prevents hair loss, the method includes the steps: (a) give Object the application beta glucan, preparation or composition product of the invention needed.

The object for applying preparation or composition of the invention is not particularly limited, and composition of the invention can be applied to people With all animals including domestic animal and pet.For example, the object of the needs is with alopecia, hypotrichosis and/or bald The men and women of hair.Animal with this kind of patient's condition can also be treated with composition of the invention.

Preferably, preparation or composition of the invention are administered in non-oral mode, for example, skin office can be passed through Portion is administered to be administered.

Composition of the invention can be coated on scalp by being directly coated with method, for example, a small amount of the composition is poured on head On skin or on finger, the substance then is wiped on scalp or on the region worked.Alternatively, conventional spraying device can be used (such as empty gas-powered spraying device or smoke devices) spraying the composition, these devices are widely used in applying perfume and beauty Hold product.

Give the methods of mode, such as the preferable coating of dusk to night certainly as invention formulation or composition pass through Skin gives invention formulation or composition, and rinses in night or retain overnight method, but be not limited to the administration mode.

Invention formulation or composition can be administered alone, or with other treatment administered in combination (as prepared same In one pharmaceutical composition).

Invention formulation or composition can also join to the known food treated or improve similar symptom or health food With.When combination, the originally usage mode of food or health food and dosage can be remained unchanged, and subsequently or simultaneously using the present invention Preparation or composition.With the combination of food or health food, the period for being also included within overlapping uses the present composition and its Its one or more of known drug.Or when the present composition and other one or more of foods or health food are combined When, their dosage when being used alone of the dose ratio of product of the present invention composition or known food or health food are lower.

Invention formulation or composition can also be combined to the known other medicines for treating or improving similar symptom.Joint When administration, originally the administration mode of drug and dosage are remained unchanged, and subsequently or simultaneously use pharmaceutical composition of the present invention.Drug The period that combination is also included within overlapping uses pharmaceutical composition of the present invention and other one or more of known drugs.Work as the present invention When pharmaceutical composition and other one or more of drugs carry out drug combination, the dosage of pharmaceutical composition of the present invention or known drug Dosage when may be than their independent medications is lower.For example, by the first pharmaceutical composition of the invention and the second pharmaceutical composition Internet of Things Close application.

The dosage of beta glucan of the invention should be appropriate really according to gender, age, baldness situation, hair situation etc. It is fixed.In general, the concentration used is 0.1mg/mL-5mg/mL or 0.5mg/mL-3mg/mL.

In an embodiment of the invention, above-mentioned preparation or composition are passed through into the methods of spraying, coating, soak warp Skin gives affected part or wishes the scalp or skin area of hair tonic, can also carry out percutaneous dosing appropriate to entire scalp.It is preferred that every It is applied on scalp/skin.It can the composition is spraying or be poured on region to be treated and can be soft with finger It wipes or is easily rinsed or combed by hair and scalp in ground.

In an embodiment of the invention, composition of the invention can be applied daily or at least be applied every three days Once.In general, this use can prevent hair loss in surrounding.Hair is generally observed after continuous use two months to 12 months Hair restores the visible difference in terms of (i.e. kainogenesis is long).Using daily or the product is used continuously once every three days can be to prevent Alopecia-stopping is simultaneously effectively facilitated hair tonic.

Main advantages of the present invention include:

(a) beta glucan of the present invention can effectively promote hair tonic and/or prevent hair loss.

(b) beta glucan of the present invention promotes the side effect of hair growing very low, and it is flat not destroy skin surface flora ecology Weighing apparatus, will not patient harm skin or make its generate drug resistance.

(c) beta glucan of the present invention can be not only used for promoting hair tonic and/or to prevent hair loss, it may also be used for remove and/or Pre- anti-dandruff, antipruritic, reparation damaged scalp or hair follicle, confrontation head infection, prevention and/or treatment scalp inflammation (such as hair follicle Scorching, seborrhea).

(d) beta glucan of the present invention is applied in combination with other hair growing medical anti-alopecias or treatment head dermatitis disease drug, can To further enhance prevention and/or therapeutic effect, there is synergistic effect.

(e) beta glucan of the present invention is the biological polyoses in pure natural source, has completely soluble or natural soluble.By In not needing and without the modification or modification of any chemistry and/or physics, beta glucan of the present invention completely remains three spiral shells The three-dimensional conformation of rotation, having effects that better hair tonic and hair losing preventing activity, this is most important for its performance.Beta glucan of the present invention With splendid stability, it can be coexisted with most of substances and keep its activity, thus application field is extensive.

(f) fundamental characteristics of beta glucan optimizing surface activating agent (such as AES) of the present invention, can be improved foam yield, And antifoaming speed is slow, foam is finer and smoother, can go deep into hair follicle and be cleaned, and increases connecing for beta glucan and hair follicle inner cell The effect of touching, playing immune enhancing, strengthen the hair hair tonic.

(g) invention formulation or composition can not only effectively promote hair tonic and/or prevent hair loss, moreover it is possible to clean Hair is nursed, removing and/or pre- anti-dandruff, antipruritic, reparation damaged scalp or hair follicle, are prevented and/or controlled confrontation head infection It treats scalp inflammation (such as epifolliculitis, seborrhea)；With good wrapped, hair can be wrapped up, in the same of effective water lock When, it protects hair and improves hair quality, there are good conditioning functions to hair, play antistatic, increase hair soft degree, improvement Dry, wet cardability, and reduce the effects of hair tangles.Especially for no silicone oil shampoo wash it is rear it is dry and astringent, be difficult to comb Drawback has splendid improvement.

Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention Rather than it limits the scope of the invention.In the following examples, the experimental methods for specific conditions are not specified, usually according to conventional strip Part, such as Sambrook et al., molecular cloning: laboratory manual (New York:Cold Spring Harbor Laboratory Press, 1989) condition described in, or according to the normal condition proposed by manufacturer.Unless otherwise stated, no Then percentage and number are weight percent and parts by weight.

Experimental method used in following embodiments is conventional method unless otherwise specified.

Material as used in the following examples, reagent, equipment etc., are commercially available unless otherwise specified.

The acquisition and measurement of embodiment 1, beta glucan

The present embodiment only by schizophyllum commune fermentation obtain beta glucan for, but not limited to this.

One, the acquisition of schizophyllum commune strain fermentating liquid

Fermentation liquid in following embodiments passes through fermentation schizophyllum commune strain (schizophyllum commune Schizophyllum commune Fr -1003, catalogue:38548^TM, it is purchased from American Type Culture Collection Center [ATCC]), specific as follows:

1, schizophyllum commune actication of culture: potato 200g/L, glucose 30g/L, sodium chloride 10g/L, agar 20g/L are made Plating medium is inoculated into schizophyllum commune strain on the plating medium, obtains plate in cultivating 7 days in 25 DEG C of constant incubators Mycelium；

2, seed activation: by potato starch 100g/L, glucose 40g/L, yeast extract 2g/L, yeast extract 2g/L, Fluid nutrient medium made of water is packed into shaking flask, and liquid amount 1/3 takes the resulting plate mycelium of step 1 to be inoculated with the shaking flask, in 25 The culture of 160rpm shake culture 7 days in DEG C constant-temperature table, as seed liquor；

3, fermented and cultured: by glucose 50g/L, sucrose 50g/L, soybean powder (Wen Ji Food Co., Ltd, Zhaoyuan, shandong city) 5g/L, yeast extract 2g/L, potassium dihydrogen phosphate 0.5g/L, bitter salt 0.5g/L, ammonium sulfate 0.5g/L, potassium nitrate 6g/L It is added in fermentor and sterilizes 15 minutes with fermentation medium made of water for 121 DEG C, then the resulting seed liquor inoculation of step 2 is above-mentioned In fermentor, 25 DEG C of constant temperature 300rpm are stirred the culture of 4Lpm aerobic fementation 8 days, obtain schizophyllary fermentating liquid.

Two, beta glucan isolate and purify and the preparation of beta glucan solution

1, the schizophyllary fermentating liquid that above-mentioned first part obtains is mixed with the distilled water of 4 times of volumes, digestion 8h at 60 DEG C, Obtain digestion liquid；

2, the digestion liquid for obtaining step 1 is centrifuged 5min in 4,000rpm, takes supernatant；By the supernatant filter of 300 mesh Cloth negative pressure filtration, takes that filter out clear liquid stand-by, as digestion clear liquid；

3, the filtered digestion clear liquid of step 2 is heated to 50 DEG C, thereto simultaneously be added 200 mesh wood activated charcoal and The cocoanut active charcoal of 8~16 mesh, the addition volume of every kind of active carbon are the 1% of digestion supernatant volume.In 50 DEG C, 350rpm condition 4h is persistently stirred down, it is stand-by after cooling, obtain the digestion clear liquid for being mixed with active carbon；It is blended with the digestion clear liquid of active carbon again Negative pressure leaching successively is carried out using 300 mesh filter clothes and SCP-321# filter plate (about 1.5 μm of pore size), filtered clear liquid is taken to wait for With；

4, Solarbio lipase (L8070,100~400U/mg of enzyme activity) is dissolved in physiological phosphate buffer, is pressed The enzyme dosage of 10U/mL lipase solution is added in filtrate prepared by step 3, after mixing evenly 40 DEG C of enzymatic hydrolysis 2h；Again will Solarbio neutral proteinase (Z8030, enzyme activity > 60U/mg) is dissolved in physiological phosphate buffer, is used by the enzyme of 60U/mL Neutral protein enzyme solutions are added in above-mentioned enzymolysis liquid amount, after mixing evenly, 40 DEG C of enzymatic hydrolysis 2h.90 DEG C of water-baths after enzymatic hydrolysis 30min is heated, it is living with enzyme deactivation, then negative pressure leaching is carried out with SCP-321# filter plate (about 1.5 μm of pore size), it takes filtered clear Liquid is stand-by；

5, the filtered clear liquid of step 4 is quickly mixed (volume ratio 1:3) with the edible ethanol of 95% concentration, is stirred, Until obtaining precipitate；Precipitate is re-dissolved to original volume again, quickly mixes (volume with the edible ethanol of 95% concentration afterwards Than for 1:3), stirring, until obtaining precipitate；

6, the precipitate for obtaining step 5 is placed in pallet with holes, is dried using 40 DEG C of Electric heat oven, until constant weight, obtains Desciccate；

7, the beta glucan dried object for obtaining above-mentioned steps 6 crushes, and weighs crushed material 5g, it is ultrapure to be dissolved in 1,000mL In water, 600rpm is maintained to stir 2h, until beta glucan sufficiently dissolves, obtains beta glucan solution；Above-mentioned beta glucan is molten Liquid carries out negative pressure leaching using 5 μm of filter membranes, and the beta glucan solution of high viscosity, high transparency that concentration is 0.5% can be obtained.

Light transmission of the beta glucan solution that the mass concentration is 0.5% under 600nm wavelength (being detected with spectrophotometer) Rate can achieve 90%, and viscosity is up to 600mPas or more at 40 DEG C.In use, β-the Portugal that can be 0.5% by the mass concentration Glycan solution is diluted with solvent appropriate.

Three, the identification and detection of beta glucan

1, the infrared spectroscopy identification of beta glucan

Above-mentioned 0.5% beta glucan solution is identified, specifically according to the Pharmacopoeia of the People's Republic of China (2010 Version) method C uses infra-red sepectrometry in two annex IV, by the sample after 105 DEG C of drying, use Fourier transform infrared spectroscopy Instrument carries out full wavelength scanner.As a result 0.5% solution that 1 second part step (7) of above-described embodiment obtains is beta glucan solution.

It is compared with the infrared spectrogram of yeast beta-dextran in yeast beta-dextran professional standard QBT 4572-2013, official Energy cumularsharolith is set to coincide substantially.As shown in Figure 1, main position of functional group are as follows:

1)3301cm^-1Nearby there is relatively strong, wider absorption peak (carbohydrate O-H key stretching vibration absworption peak)

2)2921cm^-1Nearby there is weaker absorption peak (carbohydrate c h bond stretching vibration absworption peak)

3)886cm^-1Nearby there is weaker absorption peak (carbohydrate beta comfiguration eigen vibration absorption peak)

4)1076cm^-1Nearby there is stronger absorption peak (carbohydrate C-OH, C-O-C stretching vibration absworption peak)

Fourier transform infrared spectroscopy test result shows that the product that the present embodiment obtains is beta glucan.

2, the content detection of beta glucan

Beta glucan quantitative detection is carried out to the desciccate that 1 second part step (6) of above-described embodiment obtains, is specifically pressed According to yeast beta-dextran content assaying method in yeast beta-dextran professional standard QBT 4572-2013, obtained β-Portugal is gathered Sugared dried object sample is ground to the rear progress of diameter 1.0mm or so.Beta glucan content is 99.23% in result product.

3, the hormone test of beta glucan solution

48 hormone determinations are carried out to above-mentioned 0.5% beta glucan solution, specifically according to " cosmetics safety technology is advised Model " (version in 2015) chapter 4 2.4 7 kinds of the first method of component high pressure liquid chromatography-diode array detector methods such as estriol and 40 a kind of the measurement liquid chromatogram/tandem mass spectrometry and thin-layer chromatography of glucocorticoid in GB/T24800.2-2009 cosmetics Method carries out.As a result in the 0.5% beta glucan solution that 1 second part step (7) of above-described embodiment obtains, above-mentioned hormone is not detected (the results are shown in Table 1).

1 48 hormone determinations of table

4, the viscosimetric analysis of beta glucan solution

According to the method for 1 second part step (7) of embodiment, 0.3%, 0.5%, 0.8%, 1.0% (quality volume is prepared Than) beta glucan aqueous solution, detect dynamic viscosity respectively when 25 DEG C.As a result see Table 2 for details, with mentioning for beta glucan content The viscosity of height, each sample is also incremented by, respectively 472,740,2150 and 3100mPas.

Commercial dispersants (not soluble in water) yeast beta-dextran particle (purchased from Wellmune company) is taken, 2g is accurately weighed, Deionized water mixing is added, and is settled to 200mL, the yeast beta-dextran suspension that mass volume ratio is 1.0% is obtained, in 25 DEG C when detect dynamic viscosity.As a result the dynamic viscosity of the 1.0% yeast beta-dextran suspension is 0mPas (being shown in Table 2).

Commercially available soluble yeast ss-glucan powder (purchased from Wellmune company) is taken, 2g is accurately weighed, deionization is added Water dissolution, and it is settled to 200mL, the yeast beta-dextran aqueous solution that mass volume ratio is 1.0% is obtained, is detected when 25 DEG C dynamic Power viscosity.As a result the dynamic viscosity of the 1.0% yeast beta-dextran aqueous solution is 0mPas (being shown in Table 2).

The measuring method of above-mentioned dynamic viscosity is as follows:

(1) the above-mentioned solution example of 200mL is taken, is placed in 250mL beaker；

(2) above-mentioned solution/mixed liquor sample beaker will be filled to be placed in water-bath, 25 DEG C of heat preservation 1h；

(3) rotational viscometer is used, the dynamic viscosity of each sample at 25 DEG C of detection.

5, the determination of light transmittance of beta glucan solution

According to the method for 1 second part step (7) of embodiment, 0.3%, 0.5%, 0.8%, 1.0% (quality volume is prepared Than) beta glucan aqueous solution, at 600nm wavelength measure solution light transmittance, be as a result such as shown in Table 2, the light transmission of each sample Rate is respectively 96.5%, 93.1%, 87.5% and 81.1%.

The avenabeta glucosan solution (purchased from Symrise company) for taking commercially available 1.0%, measures solution at 600nm wavelength Light transmittance, as a result light transmittance is 59.7% (being shown in Table 2).

Commercial dispersants yeast beta-dextran particle is taken, 2g is accurately weighed, deionized water mixing is added, and be settled to 200mL obtains the yeast beta-dextran suspension that mass volume ratio is 1.0%, suspension light transmission is measured at 600nm wavelength Rate, as a result light transmittance is only 1.3% (being shown in Table 2).

Commercially available soluble yeast ss-glucan powder is taken, 2g is accurately weighed, deionized water dissolving is added, and be settled to 200mL obtains the yeast beta-dextran aqueous solution that mass volume ratio is 1.0%, solution light transmittance is measured at 600nm wavelength, As a result light transmittance is 68.4% (being shown in Table 2).

The measuring method of above-mentioned light transmittance is as follows:

(1) the above-mentioned solution example of 10mL is taken to be placed in centrifuge tube；

(2) 1000rpm low-speed centrifugal 1min, with go bubble removing (two primary yeast beta glucan solution/suspension bubble-free, not Through centrifugal treating)；

(3) it carefully takes in 3mL to 1cm glass cuvette, avoids bubble；

(4) spectrophotometer is used, reference (light transmittance of deionized water is in terms of 100%) using deionized water as blank, Sample transmittance is measured at 600nm wavelength.

6, the Stability Determination of beta glucan solution

According to the method for 1 second part step (7) of embodiment, 0.5%, 0.8%, 1.0% (mass volume ratio) is prepared Beta glucan aqueous solution, after preservative is added, room temperature (not being protected from light) is placed 24 months, observes stability of solution, and detect solution Dynamic viscosity and light transmittance.As a result, above-mentioned three kinds of solution states are sufficiently stable, viscosity and light transmittance variation are little, wherein Light transmittance even increases and (is shown in Table 2).

Commercially available 1.0% avenabeta glucosan solution is taken, room temperature (not being protected from light) is placed 24 months, stability of solution is observed, And detect solution dynamic viscosity and light transmittance.As a result, the 1.0% avenabeta glucosan solution state is very unstable, room temperature Just there is solid content precipitation after placing 3 months, causes its viscosity and light transmittance that can not detect and (be shown in Table 2).

The various beta glucan solution dynamic viscosity data of table 2

Note: a is commercial dispersants yeast beta-dextran particle (not soluble in water), and b is commercially available soluble yeast ss-glucan Powder.

2 beta glucan of embodiment promotes hair growth experiment (rat)

One, experimental method

1, Wistar rat 8 are taken, it is spare；

2, respectively take 100g rosin and paraffin in small beaker, it is melt-blended in boiling water bath heating, and 70 DEG C of heat insulations are solid It is spare；

3, the weight for weighing every rat carries out intraperitoneal injection fiber crops using trichloroacetaldehyde according to the dosage of weight 0.3% It is liquor-saturated；

4, at left and right sides of rat back after being anesthetized, one piece of region is respectively chosen, uniformly smears appropriate rosin paraffin mixing Object is taken off after solidification slightly to be cooled against hair growth direction, and hairless rat dorsum skin is exposed；

5, it is divided into two groups after rat back defeathering, smears 1mg/mL glycerol and 1mg/mL respectively every rat back two sides Schizophyllum commune beta glucan (is prepared) according to 1 method of embodiment, wherein the side for smearing 1mg/mL glycerol is control group, smears The side of 1mg/mL schizophyllum commune beta glucan is experimental group；

6, it respectively carries out in every morning and afternoon once smearing operation, and observes the skin and hair growth of each group rat Situation photographs to record.

Two, experimental result

As a result as shown in Figure 2.Figure it is seen that experimental group (schizophyllum commune beta glucan group) is big after continuously smearing 9 days Hair rate of living again in mouse back is more than 90%, and control group hair lives again rate less than 10%.The result shows that schizophyllum commune beta glucan has There is significant trichogenous effect.

The fundamental characteristics of 3 beta glucan of embodiment optimization AES

One, experimental method

1, the aqueous solution of two pipe 25mL 10%AES is taken, experimental group adds 1mg/mL schizophyllum commune beta glucan (according to implementation 1 method of example is prepared), control group adds 1mg/mL deionized water.Two pipes mix respectively, after acutely shaking 30s, observe immediately Bubble production volume.

2, the aqueous solution of two pipe 25mL 10%AES is taken, experimental group adds 1mg/mL schizophyllum commune beta glucan (according to implementation 1 method of example is prepared), control group adds 1mg/mL deionized water.Two pipes mix respectively, after acutely shaking 30s, observe immediately Antifoaming speed.

3, the aqueous solution of two pipe 25mL 10%AES is taken, experimental group adds 1mg/mL schizophyllum commune beta glucan (according to implementation 1 method of example is prepared), control group adds 1mg/mL deionized water.Two pipes mix respectively, after acutely shaking 30s, observe immediately Foam sophistication.

Two, experimental result

As a result as shown in Figure 3.Fig. 3 A adds 1mg/mL schizophyllum commune beta glucan group, and foam stack height is good.Add 1mg/mL Bubble production volume is 60% after beta glucan, and control group only 20%.Fig. 3 B adds 1mg/mL schizophyllum commune beta glucan group, antifoaming speed Slowly.Addition 1mg/mL beta glucan can significantly slow down the antifoaming speed for washing shield product.It is poly- that Fig. 3 C adds 1mg/mL schizophyllum commune β-Portugal Sugared group, foam is clearly finer greasy.Fine and smooth foam abundant can go deep into hair follicle and be cleaned, and increase thin in beta glucan and hair follicle The contact of born of the same parents, the effect of playing enhancing immune, strengthen the hair hair tonic.

4 shampoo of embodiment composition and effect

One, the composition of various shampoos

The preparation of according to the form below 3 is without silicone oil shampoo, and wherein shampoo I, III, V, VII and VIII is added with splitting for different content Gill fungus beta glucan (is prepared) according to 1 method of embodiment.Shampoo II, IV and VI except without in addition to schizophyllum commune beta glucan, Other components are identical as shampoo I, III and V respectively.

Table 3 is main composition without silicon shampoo formula using beta glucan

Two, without silicon shampoo preparation method

1, first sodium sulfate of polyethenoxy ether of fatty alcohol is all added in 40% cold water, is heated to 75 DEG C, stirred to transparent；

2, EDETATE SODIUM, coconut oil list isopropanol amide, 638 thickeners, Cocoamidopropyl betaine, schizophyllum commune is added Beta glucan (being added in the shampoo of part) and -7 coco glycerides of polyethylene glycol, 75 DEG C of constant temperature stirring to it is transparent it is uniform (about Start to cool down after 10min)；

3, after stopping heating, the Polyquaternium-67 dispersed with 10% cold water, 65-70 DEG C of heat preservation 5min of constant temperature, until saturating is added It is bright uniform；

4,45 DEG C when be added remaining essence and preservative etc., adjust viscosity and pH value, stir evenly discharging.

Three, shampoo sampling is evaluated

After 28 volunteers use I, III, V, VII or VIII shampoo 10 times respectively, its synthesis of sensory evaluation improves hair Effect of matter, including cardability, pliability, glossiness etc., concrete outcome is as shown in table 4 below.

Table 4 is main composition without silicon shampoo result on trial using beta glucan

Hair problem	Cardability	Pliability	Glossiness	It is anti-dandruff	It is antipruritic	Oil-control	Anticreep
								Positive feedback number	25	28	28	23	24	28	27
Negative-feedback number	-	-	-	1	-	-	-

Fed back according to volunteer, before using commonly without silicon shampoo or II, IV and VI shampoo after, second day hair is just Start to return oil, itching of the scalp.But using addition schizophyllum commune beta glucan without silicone oil shampoo, (I, III, V, VII or VIII are washed Shampoo) after, the astringent problem of hair washing process is improved first, and foam is more abundant, cleans cleaner.Importantly, solution It has determined scared hot-tempered without silicon washing back of having one's hair wash, it is difficult to which the problem (as shown in Figure 4) combed etc., in addition hair returns the oily time from original Postpone till within 1 day 3 days, and alopecia hair loss significantly reduces.It is significantly gone the result shows that schizophyllum commune beta glucan of the present invention has Consider effect to be worth doing, hair quality becomes soft and bright, and being capable of phalacrosis prevention and hair generation.

In addition, separately there is 9 volunteers using after II, IV and VI shampoo 10 times, foam is less during discovery hair washing, holds Easily astringent, hair is easy to return oil, short-tempered difficult combing, itching of the scalp, and alopecia hair loss is not improved.

Embodiment 5 repairs the experiment of hair scale piece

One, experimental method

Experiment reagent and equipment: sterile water, 0.12mg/mL schizophyllum commune source beta glucan (according to 1 method system of embodiment It is standby to obtain), scanning electron microscope, 10cm culture dish, hair.

Experimental procedure:

(1) it takes the above-mentioned each sample of 10mL to be placed in 10cm culture dish, hair is immersed, about 5min；

(2) hair after immersion is taken out, is placed in the ware equipped with clear water, taking-up, which is dried, after elution 5min (is placed on shaking table Gently concussion elution)；

(3) by the hair Jing Guo above-mentioned processing, the situation of hair scale piece is observed under scanning electron microscope, and is photographed to record.

Two, experimental result

Electromicroscopic photograph shows that the hair of sterile water process group, rough surface, hair scale piece is in irregular tilting shape.And The hair of 5min is handled in the beta glucan solution in 12mg/mL schizophyllum commune source, surface is smooth, the closure degree of hair scale piece Significantly larger than sterile water process group (as shown in Figure 5).

Schizophyllum commune source beta glucan belongs to macromolecular biological polyoses substance, have it is good wrapped, hair can be wrapped up Silk can also repair hair scale piece while effective water lock, protect hair and improve hair quality.It is used for shampoo, hair conditioner and Hair film etc. wash shield product in, to hair have good conditioning functions, play it is antistatic, increase hair soft degree, glossiness, change Kind dry and wet cardability, and reduce the effects of hair tangles.

6 scalp nursing solution anticreep of embodiment uses experiment

One, experimental method

During on probation, volunteer is daily or hair washing every other day is primary, by the beta glucan in 0.1% schizophyllum commune source after hair washing (being prepared according to 1 method of embodiment) is sprayed on scalp and is gently massaged into absorption, observes the variation of hair loss amount.

Two, experimental result

50 volunteers are counted in total, there is different degrees of alopecia problem, including alopecia seborrheica, are worked and rested irregular de- It sends out, hereditary alopecia, alopecia caused by postpartum alopecia and some unknown causes.

50 volunteers use the scalp care essence 30 times or so, and positive feedback rate is reached for 100%.

5 beta glucan anticreep strengthen the hair result on trial of table

Access times	5 times	10 times	20 times	30 times	50 times	80 times	100 times	200 times
									Positive feedback (number)	1	39	43	50	50	50	50	50
Negative-feedback (number)	0	0	0	0	0	0	0	0

The degree of hair loss can be effectively relieved using result data statistics display, the beta glucan in schizophyllum commune source in volunteer, 70% or more volunteer is exactly apparent improvement using 10 times or so, and the scalp care essence is used for a long time, can significantly improve The case where hair loss, and facilitate hair tonic.

The experiment of 7 phalacrosis prevention and hair generation of embodiment

Volunteer A: female 29 years old, belongs to pressure alopecia, average hair loss amount 150 or so of washing hair every time；

During on probation, after which has one's hair wash once daily, by the beta glucan in 0.1% schizophyllum commune source (according to reality 1 method of example is applied to be prepared) it is sprayed on scalp and is gently massaged into absorption, observe the variation of hair loss amount.

As shown in table 6, using after beta glucan, hair loss amount is gradually decreasing volunteer A, and daily hair loss amount has been after 3 weeks Less than 100.In addition, volunteer discovery, the originally serious place of hair loss (can have been seen exposed after 3 months on probation The hypotrichosis position of scalp) many new hairs are grown, hair line reduces, and restores normal hair amount (Fig. 6) after 24 weeks.

6 volunteer of table tries out the variation of alopecia amount after schizophyllum commune beta glucan

The experiment of 8 phalacrosis prevention and hair generation of embodiment

Volunteer B: female 27 years old, belongs to the alopecia of postpartum hormonal, and average hair loss 200 or so of washing hair every time, forehead position is bright Aobvious to have bare patch appearance, alopecia degree is serious.

Volunteer B result on trial is as shown in fig. 7, hair loss amount significantly reduces after 2 weeks, and discovery bare patch position is grown after 8 weeks New hair, bare patch is by new hair covering after 24 weeks, the hair amount returned to normal after 48 weeks.

The experiment of 9 phalacrosis prevention and hair generation of embodiment

Volunteer C: male, 39 years old, the property alopecia of male sex hormone source.The property alopecia of male sex hormone source is also known as alopecia seborrheica, male Property alopecia or hereditary alopecia, patient's scalp grease excessively overflows, and being often accompanied by dandruff increases, and scalp is greasy, and itch is obvious.Male Hormone source property baldness, mostly occurs in the vigorous person between twenty and fifty of smegma, starts gradually from overhead alopecia, sprawling later and Forehead is then diffused in the entire crown.The general hair of patient is soft, and some is also with dandruff dermatitis symptom.Scalp oil Greasy and azarin ties yellow oil scab.Alopecia seborrheica is a kind of permanent alopecia, some males start to occur after development Alopecia, and then hair is greasy shinny, dandruff slowly increases, and surprise often occurs and itches, sometimes the withered tarnish of hair, as long as using hand It grabs, hair will fall off, and especially two sides frontal eminence also occurs that chronic alopecia generalisata.

Volunteer C takes the β-Portugal in the 0.3% schizophyllum commune source of 3mL or so during on probation twice, every time daily morning and evening Glycan solution (is prepared) according to 1 method of embodiment, is applied on scalp and is gently massaged into absorption.After 20 weeks, start dilute Thin microvillus is gradually healthy and strong, and density is also gradually promoted, and hair is obviously preceding dense (more as shown in Figure 8) than using after 24 weeks.

The service condition of above-mentioned human volunteer, it was confirmed that beta glucan can effectively improve hair quality, reduction/anti-anti-avulsion Hair, and promote natural on-off cycles of hair growth, it can be used for the damage of hair quality caused by preventing and treating a variety of causes, alopecia, promote hair growth and treatment spot The problem of bald equal hairs amount is reduced.

It discusses

Present invention firstly discovers that beta glucan has effects that significantly to promote hair tonic and/or prevent hair loss, especially originally The schizophyllum commune beta glucan of the high viscosity, high molecule mass that are prepared in inventive embodiments 1.Present invention discover that the promotion of beta glucan Hair tonic and/or the activity prevented hair loss are related with its degree of branching height, the amount of glucose of side chain, stereochemical structure, molecular weight System.The degree of branching of yeast dextran is indefinite, extends the side chain being made of β -1,6- glycosidic bond, the grape of side chain on main chain at random Sugared quantity is larger and indefinite, and more up to up to a hundred.Yeast dextran side chain lengths are long, strict netted knot easy to form Structure to promotion hair tonic and/or prevents hair loss effect not as good as schizophyllum commune beta glucan.Schizophyllum commune beta glucan degree of branching is moderate, side Chain only one glucose residue.

All references mentioned in the present invention is incorporated herein by reference, independent just as each document It is incorporated as with reference to such.In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can To make various changes or modifications to the present invention, such equivalent forms equally fall within model defined by the application the appended claims It encloses.

Claims

1. a kind of purposes of beta glucan, which is characterized in that promote hair tonic for (a) and/or prevent hair loss, or (b) preparation promotees Into hair tonic and/or the preparation or composition that prevent hair loss.

2. purposes as described in claim 1, which is characterized in that the beta glucan is β -1,3- glucan, it is preferable that described Beta glucan is the beta-1,3-dextran with β -1,6- branch.

3. purposes as described in claim 1, which is characterized in that the structure of the beta glucan is shown in formula I,

Wherein, l is >=0 integer, preferably 0-50, preferably 0-10, more preferably 0-3, more preferably 1-2, more preferably 1；M be >= 0 integer, preferably 0-19, preferably 0-4 are more preferably 0-1, are more preferably 0；The integer that n is >=3, preferably 30-60000, more preferably 100-10000.

4. purposes as described in claim 1, which is characterized in that the degree of branching of the beta glucan is 0.02-0.8, preferably 0.1-0.5, preferably 0.25-0.4, more preferably 0.2-0.4.

5. purposes as described in claim 1, which is characterized in that molecular weight >=2kD of the beta glucan, preferably 2kD- 40000kD, more preferably 20kD-20000kD.

6. purposes as described in claim 1, which is characterized in that the beta glucan is selected from the group: schizophyllum commune beta glucan, perfume (or spice) Mushroom beta glucan, pyrenomycetes beta glucan, grifola frondosa beta-glucose, polysaccharide of picking up the ears, mushroom beta glucan, yeast beta-dextran, Avenabeta glucosan, or combinations thereof.

7. a kind of preparation for promoting hair tonic and/or preventing hair loss, which is characterized in that the preparation includes to be used as active constituent Beta glucan.

8. the purposes of preparation as claimed in claim 7, which is characterized in that be used to prepare product selected from the group below: hair tonic and/or Anti-hair loss liquid medicine, hair tonic and/or Anti-hair loss ointment, hair tonic and/or Anti-hair loss drug or patch, shampoo, profit hair-cream or hair care Element, hair jelly, mousse, paint, hair cream, hair film, hair rinse, eyebrow regenerative agent, eyelashes regenerative agent or eyelashes nutritional agents, hair-care essence Liquid, or combinations thereof.

9. a kind of composition product, which is characterized in that the composition product includes:

(1) first chamber, the first chamber contain (a) first active constituent, and first active constituent is poly- for β-Portugal Sugar；(b) pharmaceutically, on cosmeceutical, food and/or health food be upper or the acceptable carrier of weapon exercises or excipient； And

10. a kind of hair care products, which is characterized in that the product includes: the beta glucan as active constituent.