JP3382135B2 - External preparation for hair - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION This invention relates to a hair for external agents, more particularly, Agaricus (Agaricus
blazei Murill) An external preparation for hair, which contains an extract of fruiting body as an active ingredient and is excellent in hair growth-promoting effects such as hair loss prevention and hair growth effects.

[0002]

Alopecia is a problem of poor nutritional intake, decreased physiological function of the scalp due to excessive occurrence of dandruff, subcutaneous scalp tissue, decreased metabolic function of hair follicles and hair bulbs due to decreased blood flow in peripheral blood vessels, and It is caused by various causes such as mental stress.

In order to treat and ameliorate such alopecia, estrogen, estradiol, ethinyl estradiol, and other follicular hormones, vitamin E and its derivatives, cucumber extract, garlic extract, carrot extract, aloe extract, cepharanthin, chloride Peripheral blood flow promoters such as carpronium, minoxidil, capsicum tincture, cantaris tincture, ginger tincture, peppermint oil, l-
Local stimulants such as menthol and camphor, keratolytic agents such as resorcin, salicylic acid and lactic acid, antiseborrheic agents such as pyridoxine and its derivatives, fungicides such as zinc pyrithione, benzalkonium chloride, benzethonium chloride, chlorhexidine and hinokitiol, pantothene Acid and its derivatives, placenta extract, hair root activators such as biotin and pentadecanoic acid glyceride, glycyrrhizic acid and its derivatives, β
-Anti-inflammatory agents such as glycyrrhetinic acid, allantoin, azulene, ε-aminocaproic acid and hydrocortisone, vitamin agents such as vitamins A, B ₂ , B ₁₂ and D, amino acids such as cysteine, serine, methionine, leucine and tryptophan, Many hair growth and nourishing ingredients have been used.

Furthermore, testosterone-5, which catalyzes the conversion of testosterone to active dihydrotestosterone, mainly for the purpose of treating and improving male pattern baldness.
Many have been screened for those that inhibit the activity of α-reductase. As the enzyme inhibitor, steroid substances such as progesterone and deoxycorticosterone, as well as various plant extracts are known. Further, oxendron, spironolactone, cyproterone acetate and the like are said to inhibit the binding of dihydrotestosterone to its receptor.

However, among the above-mentioned hair growth and hair-growth ingredients and testosterone-5α-reductor inhibitors which have been conventionally used, there are side effects, so that the compoundable amount is limited or the effect is insufficient, Many of them have problems such as poor stability in the external preparation for hair and difficulty in obtaining a hair of a certain quality.

[0006]

According to the present invention, when applied to the scalp, the hair root can be activated to prevent hair loss, promote hair growth and hair growth, and is highly safe for the scalp. It is to provide an external preparation.

[0007]

[Means for Solving the Problems] Various investigations were conducted to solve the above problems. As a result, Agaricus blazers
We have found that the extract of fruiting body ( ei Murill) activates hair roots, and exerts the effects of preventing hair loss and promoting hair growth and hair growth.
The extract of Agaricus blazei Murill fruiting body had stable quality with no adverse effects on the skin such as skin irritation and contact sensitization.

Agaricus blazei Murill
Is a kind of agaricaceae, agaricaceae, agaricaceae, and is also called Kawariharatake or Himematsutake. Agaricus edulis are distributed in the southeastern part of South America and South America, and in the southeastern part of Brazil, it is a kind of edible mushrooms that residents have traditionally eaten. Regarding the effectiveness of this Agaricus edulis, many reports have been made especially on its antitumor activity. For example,
Extraction of fruiting bodies or mycelium of Agaricus edulis with an aqueous solvent to obtain a polysaccharide having an antitumor effect (Japanese Patent Laid-Open Nos. 55-74797 and 55-108).
292, JP-A-64-67194, JP-A-1-67195, JP-A-6-128164, etc.), and a nucleic acid component having an antitumor effect can be obtained from the fruiting body of Agaricus edulis. (Kaihei 1-66127), that a protein polysaccharide having antitumor activity is isolated from the hot water extraction residue of Agaricus edulis (JP-A-2-7863).
No. 0), a glycoprotein having antitumor activity can be obtained from 85% ethanol residue of Agaricus mushroom (JP-A-6-9423), and ergosterol having antitumor activity present in Agaricus mushroom body. Derivatives (JP-A-1-246299) and the like are disclosed. As a physiological activity of Agaricus edulis, a liver function improving agent containing Agaricus edulis extract as an active ingredient (JP-A-2-124).
No. 829), an antiallergic action of agaricus mushroom mycelium culture extract (JP-A-1-228480), and an action of agaricus mushroom body extract for improving immunopotency (JP-A-7).
-258107) and the like have been reported. This time, the present inventors newly discovered a remarkable hair root activating action in the solvent extract of Agaricus edulis body, and completed the present invention.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described.

The Agaricu mushrooms used in the present invention
s blazei Murill) has already been widely known, and has been deposited at the Institute of Biotechnology, Institute of Industrial Science and Technology with a deposit number of No. 4731, Life Research Institute.

When the extract is obtained from the fruiting body of Agaricus edulis, either the fresh fruiting body or the dried fruiting body may be used. The extraction solvent for obtaining the extract of Agaricus mushroom body is water, ethanol, methanol,
Isopropanol, isobutanol, n-hexanol,
Alcohols such as methylamyl alcohol, 2-ethylbutanol, n-octyl alcohol, glycerin, ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, triethylene Polyhydric alcohols such as glycol, 1,3-butylene glycol, hexylene glycol or their derivatives, acetone, methyl ethyl ketone, methyl isobutyl ketone,
Mixture of one or more selected from polar solvents such as ketones such as methyl-n-propyl ketone, esters such as ethyl acetate and isopropyl acetate, ethers such as ethyl ether, isopropyl ether, n-butyl ether and the like. A solvent can be preferably used. In addition, a polar solvent added with an inorganic salt such as phosphate buffered saline or a polar solvent added with a surfactant can be used without any particular limitation. Among the above extraction solvents, one solvent or two or more mixed solvents selected from ethanol, methanol, 1,3-butylene glycol and water, and a solvent obtained by adding an inorganic salt or a surfactant to these solvents. Is preferably used.

Further, as an extraction method, a method of impregnation at room temperature, a state of being cooled or heated, an extraction method using a distillation method such as steam distillation, a raw agaricus mushroom is directly squeezed to obtain an extract. The squeezing method and the like for obtaining the above are exemplified, and these methods are used alone or in combination of two or more kinds for extraction.

The ratio of the agaricus mushroom body to the solvent at the time of extraction is not particularly limited, but the solvent is 0.5 to 1000 times the weight of the agaricus mushroom body, especially in terms of extraction operation and efficiency. It is preferably 0.5 to 100 times by weight. Further, the extraction temperature is conveniently in the range of room temperature to the boiling point of the solvent or less under normal pressure, and the extraction time is preferably in the range of 2 hours to 2 weeks, although it varies depending on the extraction temperature and the like.

The Agaricus mushroom body extract thus obtained can be used as it is, but purification operations such as deodorization, decolorization, concentration, etc. are carried out within the range where the activity of activating hair mother cells is not lost. Or may be used as a fractionated product using column chromatography or the like.
These extracts, purified products, and fractions can also be made into a dry solid by removing the solvent from them, and are further solubilized in a solvent such as alcohol, or in the form of an emulsion, an external preparation for hair. Can be added to.

The amount of these Agaricus edulis extract extracted into the external preparation for hair depends on its effect and odor when added,
Considering the color tone, it is desirable that the concentration range is 0.001 to 20% by weight. If the blending amount is less than 0.001% by weight, a sufficient hair root activating effect cannot be obtained, but it is not necessary to blend too much, and if it exceeds 20% by weight, the stability of the external preparation for hair is affected. It may have an effect.

In the external preparation for hair according to the present invention, in addition to the active ingredient Agaricus edulis extract, the estrogen such as estrone, estradiol, ethinyl estradiol, etc., in order to further enhance the hair-growth and hair-promoting effect. , Vitamin E and its derivatives, cucumber extract, garlic extract, carrot extract, aloe extract, cepharanthin, carpronium chloride, minoxidil and other peripheral vascular blood flow promoters, capsicum tincture, cantharisin tincture,
Topical stimulants such as ginger tincture, peppermint oil, l-menthol and camphor, keratolytic agents such as resorcin, salicylic acid and lactic acid, antiseborrheic agents such as pyridoxine and its derivatives, zinc pyrithione, benzalkonium chloride, benzethonium chloride, Chlorhexidine, hinokitiol and other fungicides, pantothenic acid and its derivatives, placenta extract, biotin, pentadecanoic acid glyceride and other hair root activators, glycyrrhizinic acid and its derivatives, β-glycyrrhetinic acid, allantoin, azulene, ε-aminocaproic acid,
Anti-inflammatory agents such as hydrocortisone, vitamins A, B ₂ , B ₁₂ ,
Vitamins such as D, cysteine, serine, methionine,
Amino acids such as leucine and tryptophan can be mixed at the same time. Further, steroid substances such as progesterone and deoxycorticosterone, bicycloheptenone derivatives, phenoxybutanoic acid derivatives, tocopheryl quinone, tropolone derivatives, potassium bromate, cinnamic acid amide derivatives containing pentyl or neopentyl groups, and testosterone such as ubiquinone. 5α-reductase inhibitors, unsaturated fatty acids, cyclooxygenase inhibitors such as randic acid, hemlock, mussel, peppermint, sage, thyme, perilla, peppermint, mannen wax,
A plant extract having a testosterone 5.alpha.-reductase inhibitory action such as Arnica, kawara mugwort, cuckold thistle, hops, etc. can also be blended at the same time.

Further, within a range that does not impair the effects of the present invention, various components generally used as an external preparation for hair,
For example, avocado oil, palm oil, peanut oil, rice bran oil, jojoba oil, orange luffy oil, macadamia nut oil, squalane, evening primrose oil, sesame oil, sunflower oil, safflower oil, carrolla oil, carnauba wax, paraffin wax, Oils such as lanolin, diisostearyl malate, isostearyl alcohol, liquid paraffin, glycerin, diglycerin, polyglycerin, sorbit, polyethylene glycol, 1,3-butylene glycol, collagen, hyaluronic acid and other moisturizers,
2-Hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, benzophenone derivatives such as 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium salt, paraaminobenzoic acid, ethyl paraaminobenzoate , Paradimethylbenzoic acid octyl and other paraaminobenzoic acid derivatives, paramethoxycinnamate-2-ethylhexyl, diparamethoxycinnamate mono-
Methoxycinnamic acid derivatives such as glyceryl 2-ethylhexanoate, salicylic acid derivatives such as octyl salicylate and myristyl salicylate, urocanic acid, 4-tert-butyl-4'-methoxydibenzoylmethane, 2- (2'-hydroxy-5 UV absorbers such as'-methylphenyl) benzotriazole, guar gum, locust bean gum, carrageenan, quince seed, pectin, mannan, xanthan gum, dextran, curdlan, natural polysaccharides such as hyaluronic acid,
Animal-based polymers such as gelatin, casein, albumin and collagen, cellulosic semi-synthetic polymers such as methyl cellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose and carboxymethyl cellulose, starch-based semi-polymers such as soluble starch, carboxymethyl starch and methyl starch. Synthetic polymer, alginic acid propylene glycol ester, alginic acid-based semi-synthetic polymer such as alginate, polyvinyl alcohol,
Synthetic polymers such as polyvinylpyrrolidone, carboxyvinyl polymer, sodium polyacrylate, polyethylene oxide, water-soluble polymers such as bentonite, laponite, inorganic polymers such as colloidal alumina, dibutylhydroxytoluene, butylhydroxyanisole, gallic acid ester Antioxidants such as, higher fatty acid soaps, alkyl sulfate ester salts, polyoxyethylene alkyl ether sulfate salts, acylmethyl taurine salts, alkyl ether phosphate ester salts, anionic surfactants such as acyl amino acid salts, alkyl trimethyl ammonium chloride, Cationic surfactants such as dialkyldimethylammonium chloride and benzalkonium chloride, betaine alkyldimethylaminoacetate, betaine alkylamidodimethylaminoacetate, 2-alkyl-N- Amphoteric surfactants such as ruboxymethyl-N-hydroxyethyl imidazolinium betaine, polyoxyethylene alkyl ether, polyoxyethylene alkylphenyl ether, polyoxyethylene polyoxypropylene glycol, polyoxyethylene polyoxypropylene alkyl ether, polyoxyalkylene Fatty acid glycerin ester, polyoxyalkylene sorbitan ester, sorbit oligomeric tetraester, sorbit oligomeric hexaester, polyethylene glycol ester, polyoxyalkylene glycerin fatty acid ester, polyoxyalkylene sorbitan fatty acid ester, sucrose fatty acid ester, alkylolamide Nonionic surfactants such as fatty acid amide, ethylenediaminetetraacetic acid sodium salt, sodium polyphosphate Sequestering agents for um, citric acid, sodium metaphosphate, succinic acid, gluconic acid, etc., zinc oxide, allantoin hydroxyaluminum, aluminum chloride, tannic acid, astringents such as citric acid, lactic acid, etc., cooling of menthol, camphor, etc. Agents, antihistamines such as diphenhydramine hydrochloride and chlorpheniramine maleate, keratin exfoliating / dissolving agents such as salicylic acid and resorcin, and α-hydroxy acids and the like can be added.

The present invention comprises lotions, emulsions, gels,
It can be provided in the form of cream, ointment or the like. In addition, it is also provided as a cosmetic for hair in the form of hair lotion, hairnic, hair milk, hair gel, hair cream, hair pack, hair treatment, hair shampoo, hair rinse.

[0019]

EXAMPLES First, an example of producing the Agaricus edulis fruit body extract used in the examples of the present invention will be shown.

[Production Examples 1 to 4] Using the extraction solvents shown in Table 1, agaricus mushroom body extracts were prepared. The dried Agaricus edulis body was crushed, 10 times its weight of the solvent was added, the mixture was immersed at room temperature for 3 days, and the filtrate obtained by filtration was used as Agaricus edulis extract.

[0021]

[Table 1]

The hair follicle cell growth promoting action of Agaricus edulis extract was examined using cultured mouse hair follicle cells. The hair follicle cells isolated from the newborn mouse were cultured in Dulbecco's medium containing 10% by volume of fetal bovine serum to give a cell concentration of 1 × 10 ^4.
96-well microplates were seeded per piece / well. After culturing at 37 ° C for 24 hours, the medium was replaced with MCDB medium supplemented with Agaricus edulis extract. After culturing for 4 days, cell activity was measured by the MTT reduction method. A system in which the Agaricus edulis extract was not added was used as a control, and the value was set to 100 to express the cell activity.

In the MTT reduction method, NADH produced by the action of dehydrogenase existing in the mitochondria of cells on the substrate
Is a measurement method that utilizes the ring opening of the tetrazolium ring of MTT (2- (4,5-dimethyl-2-thiazolyl) -3,5-diphenyltetrazolium bromide) added to the system. Cellular activity is determined by measuring the blue-colored pharmazan produced by the ring by the absorbance at 560 nm.

[0024]

[Table 2]

As is clear from Table 2, the Agaricus edulis extract shown in Production Examples 1 to 4 was shown to significantly enhance the cell activity of mouse-derived cultured hair follicle cells.

Further, the hair growth promoting action of the Agaricus edulis extract was examined using C57 / Black mice. C57 / Black mouse back with hair clipper about 2 ×
The hair was shaved to a size of 4 cm, treated with a depilatory foam to completely remove hair, and after 24 hours, 1% of a 5% ethanol solution of Agaricus edulis extract shown in Production Example 1 to Production Example 4 was used. 0.1 ml of once a day was continuously applied for 20 days. On the last day of the test, all the hair that had grown was extracted and the total weight was measured. The test was conducted with 10 animals per group, and the average values are shown in Table 3.
At that time, a group to which only the solvent ethanol was applied was used as a control.

[0027]

[Table 3]

As is clear from Table 3, the Agaricus edulis extract shown in Production Examples 1 to 4 was shown to have a superior hair growth promoting effect as compared with the control.

[Examples 1 to 4] Lotion for hair A lotion for hair was prepared according to the following formulation, using the extract of Agaricus edulis mushroom shown in Table 4. (Prescription) (1) Ethanol 30.0 (wt%) (2) Hydroxyethylcellulose 1.0 (3) Agaricus mushroom body extract 0.5 (4) Methyl paraoxybenzoate 0.1 (5) Purified water 68 .4 Production method: (1) to (5) are mixed to make uniform.

[0030]

[Table 4]

The hair nourishing effect of each of Examples 1 to 4 was tested. At that time, a lotion for hair was prepared by substituting purified water for the Agaricus edulis extract in the above Example, and Comparative Example 1
Was similarly tested. The usage test was carried out by using 20 men and women panelists who are worried about thinning hair as a group and using them twice a day continuously for 6 months, and performing sensory evaluation on the improvement status of thinning hair and the presence or absence of irritation during use. It was The sensory evaluation result is
Regarding the improvement status of thinning hair, “Improved; 5 points”,
"Slightly improved; 4 points", "No change; 3 points", "Slightly worsened; 2 points", "Deteriorated; 1 point", No irritation when using "5 points", " Not so much; 4 points ",
"I can't say either; 3 points", "Somewhat; 2 points",
It was scored as "yes; 1 point", and the average value of 20 people was calculated. The results are shown in Table 5.

[0032]

[Table 5]

As is clear from Table 5, in the group used in Examples of the present invention, a clear tendency to improve thinning hair was recognized. On the other hand, in Comparative Example 1 in which the Agaricus edulis extract was not blended, no user had improved thinning hair. Also, regarding the feeling of stimulation during use,
Examples 1 to 4 of the present invention and Comparative Example 1 are
No particular irritation or discomfort was found.

During the above usage period, Example 1
No deterioration or deterioration of the formulation was observed in any of Examples 4 to 6, and even in the panelists using each Example,
Neither skin irritation reactions such as erythema or edema nor skin sensitization reactions were observed.

[Example 5] Cream for hair (1) Liquid paraffin 15.0 (% by weight) (2) Vaseline 15.0 (3) Beeswax 2.0 (4) Polyoxyethylene (50EO) hydrogenated castor oil 3 .0 (5) glycerin 5.0 (6) carboxyvinyl polymer 0.1 (7) xanthan gum 0.1 (8) disodium ethylenediaminetetraacetate 0.1 (9) purified water 57.7 (10) sodium hydroxide 0.5 (11) Agaricus edulis extract (Production Example 1) 0.5 (12) Ethanol 1.0 Production method: The oil phases of (1) to (3) are dissolved by heating to 80 ° C.
On the other hand, the water phase components of (4) to (9) are mixed, dissolved by heating, and heated to 80 ° C.
And The oil phase is added to the water phase while stirring, and the resulting mixture is uniformly emulsified with a homogenizer, and (10) is added. 30 ℃ after cooling
(11) is dissolved in (12) and added.

[Example 6] Gel for hair (1) Carboxyvinyl polymer 0.7 (wt%) (2) Polyvinylpyrrolidone 2.0 (3) Glycerin 5.0 (4) Sodium hydroxide 0.2 ( 5) Ethanol 10.0 (6) Ethylenediaminetetraacetic acid disodium 0.1 (7) Purified water 78.7 (8) Agaricus mushroom body extract (Production Example 2) 0.3 (9) Ethanol 3.0 Production method : Disperse (1) into parts of (3) and (7). to this
(2), (4) to (6) are dissolved in the rest of (7) and added.
Add (8) and (9).

[Example 7] Set lotion (1) Polyvinylpyrrolidone-vinyl acetate copolymer 5.0 (% by weight) (2) Methyl paraoxybenzoate 0.1 (3) Perfume 0.2 (4) Agaricus mushroom Fruit Body Extract (Production Example 3) 0.4 (5) Ethanol 30.0 (6) Polyoxyethylene-modified Silicone 0.5 (7) Glycerin 2.0 (8) Purified Water 61.8 Production Method: (1) ~ (4) are added to (5) in sequence and dissolved. on the other hand
The aqueous phases (6) to (8) are mixed and dissolved, and then added to the above solution and mixed to homogenize.

[Example 8] Hair liquid (1) Ethanol 50.0 (wt%) (2) Polyoxypropylene (40PO) butyl ether 20.0 (3) Polyoxyethylene (50EO) hydrogenated castor oil 1.0 ( 4) Methyl paraoxybenzoate 0.15 (5) Agaricus mushroom body extract (Production Example 4) 0.5 (6) Perfume 0.1 (7) Blue No. 1 (1% aqueous solution) 0.1 (8) Purified water 28.15 Manufacturing method: Components (2) to (8) are sequentially added to (1) and dissolved,
Homogenize.

[Example 9] Water grease (1) Carboxyvinyl polymer 0.5 (wt%) (2) Glycerin 50.0 (3) Purified water 38.6 (4) Polyoxyethylene (20EO) 0.2 Octyldodecyl ether (5) Sodium hydroxide 0.1 (6) Ethanol 10.0 (7) Perfume 0.2 (8) Agaricus mushroom body extract (Production Example 1) 0.2 (9) Agaricus mushroom body Extract (Production Example 3) 0.2 Production method: (1) is dispersed in (2), (4) is added to dissolved (3), and the mixture is heated and dissolved to homogenize. After cooling, it is neutralized with the component (5), and (7) to (9) are dissolved in (6) and added.

[Example 10] Hair foam (stock solution formulation) (1) Silicone oil 5.0 (wt%) (2) Polyoxyethylene (50EO) hydrogenated castor oil 1.0 (3) Dipropylene glycol 7.0 (4) Cationized cellulose 3.0 (5) Purified water 68.35 (6) Ethanol 15.0 (7) Methyl paraoxybenzoate 0.15 (8) Agaricus mushroom body extract (Production Example 2) 5 (Filling prescription) Undiluted solution 90.0 Liquefied petroleum gas 10.0 Manufacturing method: (1) is added to the melts of (2) and (3), and the mixture is homogenized with a homogenizer. This is added to the solution of (4) in (5), and then (7) and (8) are dissolved in (6) and added to and mixed with each other to prepare a stock solution. For filling, the stock solution is filled in a can, and after installing a valve, it is filled with liquefied petroleum gas.

[Example 11] Hair shampoo (1) Polyoxyethylene (3EO) lauryl sulfate 30.0 (wt%) ester sodium salt (30 wt% aqueous solution) (2) Lauryl sulfate ester sodium salt 10.0 (30 Wt% aqueous solution) (3) Coconut oil fatty acid diethanolamide 4.0 (4) Glycerin 1.0 (5) Methyl paraoxybenzoate 0.2 (6) Ethylenediaminetetraacetic acid disodium 0.25 (7) Purified water 52. 85 (8) Ethanol 1.0 (9) Perfume 0.2 (10) Agaricus mushroom body extract (Production Example 4) 0.5 Production method: (7) is heated to 70 ° C., and (1) to (6) ) Is added and dissolved uniformly, and then cooled, and (9) and (10) are dissolved in (8) and added.

[Example 12] Hair rinse (1) Silicone oil 3.0 (wt%) (2) Liquid paraffin 1.0 (3) Cetanol 1.5 (4) Stearyl alcohol 1.0 (5) Stearyl trimethyl chloride Ammonium 0.7 (6) Glycerin 3.0 (7) Methyl paraoxybenzoate 0.2 (8) Purified water 87.2 (9) Ethanol 2.0 (10) Agaricus mushroom body extract (Production Example 2) 0.3 (11) Perfume 0.1 Manufacturing method: The oil phase components (1) to (4) and the aqueous phase components (5) to (8) are mixed and dissolved, and heated to 70 ° C. The oil phase is added to the aqueous phase to emulsify, and after cooling, (10) and (11) are dissolved in (9) and added.

[0043]

As described in detail above, the agaricus mushroom ( Aga
The external preparation for hair containing the extract of ricus blazei Murill) fruiting body has excellent hair growth effects such as hair loss prevention effect and hair growth effect, as well as stability and safety.

─────────────────────────────────────────────────── ─── Continuation of the front page (56) Reference JP-A-5-345726 (JP, A) JP-A-7-316026 (JP, A) (58) Fields investigated (Int.Cl. ⁷ , DB name) A61K 7/00-7/50 JISST file (JOIS) CA (STN)

Claims (2)

(57) [Claims] 1. Agaricus blazei Murill
An external preparation for hair comprising an extract of fruiting body. 2. Agaricus blazei Murill
An external preparation for hair comprising a polar solvent extract of fruiting bodies.