CN114010566B

CN114010566B - Hair conditioner with hair growth function and preparation method thereof

Info

Publication number: CN114010566B
Application number: CN202111572652.9A
Authority: CN
Inventors: 刘子抄; 黄孟军; 刘意
Original assignee: Guangzhou Huaxia Biopharmaceutical Co ltd
Current assignee: Guangzhou Huaxia Biopharmaceutical Co ltd
Priority date: 2021-12-21
Filing date: 2021-12-21
Publication date: 2023-04-28
Anticipated expiration: 2041-12-21
Also published as: CN114010566A

Abstract

The invention discloses a hair conditioner with a hair growing function and a preparation method thereof. The hair conditioner is composed of the following raw materials in parts by weight: 5 to 10 parts of cationic surfactant, 0.05 to 0.1 part of thickener, 0.1 to 0.5 part of cationic conditioner, 3 to 5 parts of moisturizing grease, 1 to 2 parts of nutrient substances, 0.05 to 0.1 part of chelating agent, 0.05 to 0.1 part of preservative, 0.2 to 0.3 part of pH regulator, 30 to 80 parts of water and 10 to 30 parts of traditional Chinese medicine extract. The hair conditioner disclosed by the invention is simple in formula, good in compatibility of all components and high in activity of active ingredients, and the oxidation resistance and 5 alpha-reductase inhibition rate of the traditional Chinese medicine extract are increased through the ionic liquid, so that the hair conditioner has the effects of preventing alopecia and promoting hair growth.

Description

Hair conditioner with hair growth function and preparation method thereof

Technical Field

The invention belongs to the technical field of daily cosmetics, and particularly relates to a hair conditioner with a hair growth function and a preparation method thereof.

Background

The hair is an accessory fibrous tissue of a human body and is also an important component of the human body, so that the hair has the physiological functions of keeping warm and preventing sun, and can beautify the image of a person. Typically, a normal person has about 10 to 15 tens of thousands of hairs on his/her head, and 50 to 150 hairs are shed on average per day. With the development of society, hair perming and dyeing are increasingly popular, and more people face the risk of alopecia in combination with internal and external factors such as environment and pressure. Although hair loss does not pose a threat to the physical health of a person, hair loss affects the mental and physical image of a person.

Hair conditioners are the most commonly used daily chemical products for conditioning hair. It is generally believed that hair has a certain amount of negative charge, and after the shampoo is used, the anionic surfactant, which is the detersive ingredient in the shampoo, causes the hair to have more negative charge, is prone to static electricity, increases friction, and causes inconvenient combing. The cationic surfactant in the conditioner can neutralize the residual anionic substances, and a uniform monomolecular film is left, so that mechanical damage and chemical damage of hair can be repaired to a certain extent. Functional components, such as traditional Chinese medicine extracts, for promoting hair growth in the hair conditioner can enable the hair conditioner to have the hair growth function while improving the hair quality.

CN 109199938A discloses a hair blacking conditioner, which comprises the following raw materials in parts by weight: 3 parts of lanonol ether, 1 part of animal hydrolyzed protein, 2 parts of dioctadecyl dimethyl ammonium chloride, 1 part of vitamin A, 8 parts of glyceryl stearate and polyethylene glycol magnesium stearate, 2 parts of glycerol, 2 parts of silk peptide, 1 part of glutamic acid, 1 part of antioxidant, 20 parts of liquid paraffin, 55 parts of water and 4 parts of mulberry extract. The hair blackening conditioner prepared by the invention can play multiple roles of hair care, hair blackening and hair growth.

CN 113476354A discloses an anti-hair loss hair-growing conditioner, which comprises the following components in percentage by mass: 1 to 6 percent of thickening agent, 1 to 2 percent of cationic surfactant, 1 to 10 percent of proline, 0.1 to 0.5 percent of cacumen biotae extract, 0.1 to 0.5 percent of ginseng extract, 0.1 to 1 percent of ginger extract, 0.1 to 1 percent of cordyceps militaris extract, 0.1 to 1 percent of astragalus membranaceus root extract, 0.1 to 2 percent of saccharide isomer and the balance of water. The hair conditioner disclosed by the invention is mild and low in stimulation, high in transdermal absorption rate, capable of controlling oil convergence, moisturizing, nourishing scalp, strengthening hair roots, inhibiting bacteria, diminishing inflammation, regulating skin water-oil balance, maintaining scalp environment steady state, activating hair growing cell proliferation and differentiation, repairing hair follicle structure, reconstructing skin barrier, effectively fixing and nourishing hair, promoting hair growth and reducing hair loss and hair breakage. After the hair conditioner is used, the hair is smooth and glossy, fresh and fluffy, is not easy to be frizzy and knotted, and is easy to comb.

CN 108272707A discloses a Chinese herbal medicine hair conditioner and a preparation method thereof, wherein the hair conditioner is prepared from the following raw materials in parts by mass: 1 to 60 parts of ginseng, 1 to 60 parts of rhizoma polygonati, 1 to 30 parts of polygonum multiflorum and 1 to 30 parts of ginger. The invention uses the theory of traditional Chinese medicine and the research of modern traditional Chinese medicine as guidance, carries out the formulation of Chinese herbal medicines, and plays the advantages of the traditional Chinese medicine. The prepared Chinese herbal medicine hair conditioner plays roles of blackening hair, growing hair, preventing alopecia, relieving itching, nourishing hair roots and scalp by virtue of pure plants, has no anaphylactic reaction after use, has no toxic or side effect, can enable the hair to be smooth and flexible, meets the requirements of people on health maintenance, and is suitable for long-term use.

In the prior art, the hair conditioner with the hair growing function usually realizes the hair growing effect by compounding two or more traditional Chinese medicine extracts, and the prior art has less research on promoting the hair growing effect of traditional Chinese medicine components.

Disclosure of Invention

In view of the defects of the prior art, the technical problem to be solved by the invention is to provide a hair conditioner with a hair growing function and a preparation method thereof.

In order to achieve the purpose, the invention provides a hair conditioner with a hair growth function, which comprises the following raw materials in parts by weight: 5 to 10 parts of cationic surfactant, 0.05 to 0.1 part of thickener, 0.1 to 0.5 part of cationic conditioner, 3 to 5 parts of moisturizing grease, 1 to 2 parts of nutrient substances, 0.05 to 0.1 part of chelating agent, 0.05 to 0.1 part of preservative, 0.2 to 0.3 part of pH regulator, 30 to 80 parts of water and 10 to 30 parts of traditional Chinese medicine extract.

Preferably, the cationic surfactant is at least one of cetyltrimethylammonium chloride, octadecyltrimethylammonium chloride, dioctadecyl dimethyl ammonium chloride and behenyl trimethyl ammonium chloride.

The thickener is at least one of cetostearyl alcohol, carboxymethyl cellulose, propylene glycol alginate and sodium starch phosphate.

The cation regulator is at least one of guar gum hydroxypropyl trimethyl ammonium chloride, polyquaternium 7, polyquaternium 10 and polyquaternium 28.

The moisturizing grease is at least one of jojoba oil, olive oil, sweet almond oil and grape seed oil.

The nutrient substance is at least one of D-panthenol, stearyl dimethyl ammonium hydroxypropyl hydrolyzed wheat protein and cocoyl dimethyl ammonium hydroxypropyl hydrolyzed wheat protein.

The chelating agent is at least one of EDTA-disodium, EDTA-tetrasodium and sodium citrate.

The preservative is at least one of phenoxyethanol, chlorpheniramine, pinus koraiensis and methylisothiazolinone.

The pH regulator is at least one of citric acid, gallic acid and malic acid.

The traditional Chinese medicine extract is a composite extract of polygonum multiflorum, biota orientalis leaves and glossy privet fruits, and the preparation method comprises the following steps:

(1) Pulverizing cleaned and dried Polygoni Multiflori radix, folium Platycladi and fructus Ligustri Lucidi respectively, and mixing to obtain Chinese medicinal powder;

(2) Adding the traditional Chinese medicine powder obtained in the step (1) into an ethanol aqueous solution, extracting, and then filtering, concentrating and diluting to obtain a traditional Chinese medicine extract.

Polygonum multiflorum is a dry root tuber of Polygonum multiflorum (Polygonum multiflorum Thunb.) of Polygonaceae, and has been an important drug for treating white hair and alopecia since the ancient efficacy of blackening hair. A plurality of documents at home and abroad prove that the polygonum multiflorum can obviously promote the growth of scalp hair follicle cells cultured in vitro, and the effective rate of treating patients with alopecia areata and seborrheic alopecia is superior to that of similar clinical Chinese patent medicines.

The cacumen Platycladi is dried tender branch and leaf of Platycladus orientalis (Platycladus orientalis (L.) Franco) belonging to Cupressaceae, and can be used as a common Chinese medicine for treating alopecia. In the traditional Chinese medicine prescription or prescription, external smearing and other means are generally adopted, and the effects of treating alopecia are achieved by means of promoting blood microcirculation of scalp parts, increasing cell nutrients, stimulating hair follicle growth and the like.

Fructus Ligustri Lucidi is dry fruit of Ligustrum lucidum (Ligustrum lucidum) of Oleaceae, has flat nature, sweet and bitter taste, and has effects of nourishing yin, prolonging life, invigorating liver and kidney, clearing heat, improving eyesight, blackening hair, etc. Fructus Ligustri Lucidi is commonly used for treating dizziness, tinnitus, dim eyesight, soreness of waist and knees, internal heat, premature graying of beard and hair, etc.

The inventors conducted a plurality of experiments on the formulation of a conditioner and evaluated the effect of using the conditioner. The main components of the hair conditioner disclosed by the invention are cationic surfactant, moisturizing grease and traditional Chinese medicine extract, and the rest components mainly play a role in regulating the compatibility stability of the hair conditioner components. The cationic surfactant has the main effects of neutralizing the charge of anionic surfactant in shampoo, reducing friction force between hair fibers, and improving comb property. The moisturizing grease component is natural vegetable oil, is rich in multiple vitamins and unsaturated fatty acids, and has antioxidant effect and moisturizing effect; has good absorbability, and can promote absorption of effective substances in hair conditioner. The traditional Chinese medicine extract is three traditional Chinese medicine compound extracts of polygonum multiflorum, biota orientalis and glossy privet fruit, and is also a root source for achieving the hair growth function of the hair conditioner. Therefore, increasing the content of active substances in the traditional Chinese medicine extract is the most effective way to improve the performance of the hair conditioner.

At present, in the aspect of extraction, supercritical anti-solvent precipitation, ionic liquid and eutectic solvent extraction are used as safer, more environment-friendly and targeted plant product extraction schemes so as to take an organic solvent extraction mode with high residual toxicity and harmful to the environment. However, the supercritical antisolvent precipitation extraction requires a large instrument, and the diffusion rate of critical substances is difficult to adjust; because the traditional Chinese medicine components are very complex, the active substances are difficult to separate and difficult to determine, the difficulty of solvent selection in the eutectic solvent extraction is greatly increased, and the extracted components are close in nature and difficult to purify and separate. In the extraction of multicomponent Chinese medicine, in order to make the quantification of effective components accurate, the extraction solvent should be selected to consider compounds of different polarities. Ionic liquids are complex systems consisting of the organic cations imidazolium, pyridinium, ammonium) and organic or inorganic anions (tetrafluoroborate, acetate, bromide anions and chloride anions), which have a range of unique properties compared to traditional organic solvents due to the independent nature of the ions and the diversity of existing interactions within the molecule. In some prior art, ionic liquid is added in the extraction of traditional Chinese medicines, so that the extraction efficiency of carotenoid, furocoumarin and protein is enhanced.

Based on the above, the invention adds ionic liquid in the process of composite extraction of three traditional Chinese medicines of polygonum multiflorum, biota orientalis and glossy privet fruit for a large number of experiments, and discovers that the imidazole acetic acid and imidazolium ionic liquid has an extraction enhancement effect on composite traditional Chinese medicine extraction, and can improve the polarity of effective substances of the composite traditional Chinese medicine extraction species, thereby enhancing the hair growth effect of the traditional Chinese medicine extract.

Benzethonium chloride is a novel preservative and is widely used as a preservative and a bactericide in the fields of daily chemicals, medicines and the like. The inventor finds that adding a certain amount of benzethonium chloride into the mixed extraction system can enhance the extraction efficiency of the traditional Chinese medicine compound, and enhance the elimination rate of the traditional Chinese medicine compound on free radicals.

Further preferably, the preparation method of the traditional Chinese medicine extract comprises the following steps:

s1, respectively cleaning polygonum multiflorum, biota orientalis leaves and glossy privet fruit, drying until the water content is 3-5%, crushing, sieving with a 300-500 mesh sieve to obtain powder, and then mixing to obtain traditional Chinese medicine powder;

s2, mixing the materials according to a feed liquid ratio of 1g: (10-15) mL, mixing the traditional Chinese medicine powder obtained in the step S1 with 50-70 wt.% of ethanol water solution under stirring, adding ionic liquid, heating and reflux-extracting for 2-5 h, and filtering while the mixture is hot; concentrating the filtrate, freeze drying, and dispersing in water to obtain the Chinese medicinal extract.

Most preferably, the preparation method of the traditional Chinese medicine extract comprises the following steps:

s2, mixing the materials according to a feed liquid ratio of 1g: (10-15) mL, mixing the traditional Chinese medicine powder obtained in the step S1 with 50-70 wt.% of ethanol water solution under stirring, adding ionic liquid and benzethonium chloride, heating to 60-70 ℃, carrying out reflux extraction for 2-5 h, and filtering while the mixture is hot; concentrating the filtrate to 1/4-1/6 of the original volume, freeze-drying at-45 to-55 ℃ for 24-36 h to obtain powder, and dispersing the powder in water to obtain a solution with the mass concentration of 200-500 mg/mL, namely the traditional Chinese medicine extract.

Preferably, the mass ratio of the polygonum multiflorum, the biota orientalis leaves and the glossy privet fruit in the traditional Chinese medicine powder in the step S1 is (3-5): (2-4): 1.

preferably, the stirring rate in the step S2 is 300-500 r/min.

Preferably, the mass of the ionic liquid in the step S2 is 0.02-0.05 of the traditional Chinese medicine powder.

Preferably, the ionic liquid in the step S2 is one of 1-ethyl-3-methylimidazolium tetrafluoroborate or 1-ethyl-3-methylimidazolium acetate.

Further preferably, the ionic liquid in step S2 is 1-ethyl-3-methylimidazolium tetrafluoroborate and 1-ethyl-3-methylimidazolium acetate in a mass ratio of 1: (2-4) and mixing.

Preferably, the mass of the benzethonium chloride is 0.01-0.02 of that of the traditional Chinese medicine powder.

The invention also provides a preparation method of the hair conditioner, which comprises the following steps:

t1, weighing all raw materials according to a formula;

t2, stirring the cationic surfactant, the cationic conditioning agent, the nutrient substances, the chelating agent and the water for 5-10 min at the rotating speed of 300-500 r/min, and heating to 60-80 ℃ to obtain a system I;

t3, mixing the moisturizing grease, the thickener and the traditional Chinese medicine extract, heating the system to 60-80 ℃, and stirring for 5-10 min at the rotating speed of 100-300 r/min; then adding the mixture into the system I obtained in the step T2, homogenizing the mixture for 2 to 5 minutes at 8000 to 12000r/min at the temperature of between 60 and 80 ℃ to obtain a system II;

and T4, naturally cooling the temperature of the system II obtained in the step T3 to 40-50 ℃, adding a preservative and a pH regulator, and stirring for 10-20 min at the rotating speed of 100-300 r/min to obtain the hair conditioner with the hair growing function.

The invention has the beneficial effects that:

the hair conditioner disclosed by the invention is simple in formula, good in compatibility of all components and high in activity of active ingredients, and the oxidation resistance and 5 alpha-reductase inhibition rate of the traditional Chinese medicine extract are increased through the ionic liquid, so that the hair conditioner has the effects of preventing alopecia and promoting hair growth.

Detailed Description

The invention uses material introduction:

polygonum multiflorum, anhui, a country of origin.

The arborvitae twig and leaf have the advantage of being easy to use in the mountain east.

Fructus Ligustri Lucidi, yunnan Kunming.

Test mice, C57BL mice, male, 20 days old, purchased from Gibbs laboratory animal technology, inc.

The remaining non-mentioned starting materials are all common in the art.

Example 1

A hair conditioner with hair growth function is prepared by:

t1, weighing 25g of cetyltrimethylammonium chloride, 75g of behenyl trimethyl ammonium chloride, 0.5g of cetostearyl alcohol, 2g of guar gum hydroxypropyl trimethyl ammonium chloride, 50g of sweet almond oil, 5g of D-panthenol, 10g of cocoyl dimethyl ammonium hydroxypropyl hydrolyzed wheat protein, 1g of EDTA-disodium, 0.5g of kathon, 2g of citric acid, 700g of water and 150g of traditional Chinese medicine extract;

t2, mixing cetyl trimethyl ammonium chloride, behenyl trimethyl ammonium chloride, guar gum hydroxypropyl trimethyl ammonium chloride, coco dimethyl ammonium hydroxypropyl hydrolyzed wheat protein, EDTA-disodium and water for 5min at the rotating speed of 450r/min, and heating to 70 ℃ to obtain a system I;

t3 mixing oleum Armeniacae amarum, cetostearyl alcohol, D-panthenol and Chinese medicinal extract, heating to 70deg.C, and stirring at 150r/min for 10min; then adding the mixture into the system I obtained in the step T2, homogenizing the mixture at the speed of 8000r/min for 5min at the temperature of 70 ℃ to obtain a system II;

and T4, naturally cooling the temperature of the system II obtained in the step T3 to 50 ℃, adding the pinus koraiensis and the citric acid, and stirring for 15min at the rotating speed of 200r/min to obtain the hair conditioner with the hair growing function.

The preparation method of the traditional Chinese medicine extract comprises the following steps:

s1, respectively cleaning polygonum multiflorum, biota orientalis leaves and glossy privet fruit, drying until the moisture content is 5%, crushing and sieving by a 325-mesh sieve to obtain powder, and mixing the polygonum multiflorum powder, the biota orientalis leaves powder and the glossy privet fruit powder according to the mass ratio of 5:2:1, mixing to obtain traditional Chinese medicine powder;

s2, mixing 100g of the traditional Chinese medicine powder obtained in the step S1 with 1500mL of 60wt.% ethanol water solution at a rotating speed of 400r/min, adding 5g of 1-ethyl-3-methylimidazolium tetrafluoroborate, heating to 65 ℃, carrying out reflux extraction for 5h, and filtering while the mixture is hot; concentrating the filtrate to 1/6 of the original volume, freeze-drying at-45deg.C for 36h, and dispersing the obtained solid powder in water to obtain 200mg/mL solution, which is the Chinese medicinal extract.

Example 2

A hair conditioner with hair growth function is prepared by:

s2, mixing 100g of the traditional Chinese medicine powder obtained in the step S1 with 1500mL of 60wt.% ethanol water solution at a rotating speed of 400r/min, adding 5g of 1-ethyl-3-methylimidazole acetate, heating to 65 ℃, extracting under reflux for 5h, and filtering while the mixture is hot; concentrating the filtrate to 1/6 of the original volume, freeze-drying at-45deg.C for 36h, and dispersing the obtained solid powder in water to obtain 200mg/mL solution, which is the Chinese medicinal extract.

Example 3

A hair conditioner with hair growth function is prepared by:

s2, mixing 100g of the traditional Chinese medicine powder obtained in the step S1 with 1500mL of 60wt.% ethanol water solution at a rotating speed of 400r/min, adding 1g of 1-ethyl-3-methylimidazolium tetrafluoroborate and 4g of 1-ethyl-3-methylimidazolium acetate, heating to 65 ℃, carrying out reflux extraction for 5h, and filtering while the mixture is hot; concentrating the filtrate to 1/6 of the original volume, freeze-drying at-45deg.C for 36h, and dispersing the obtained solid powder in water to obtain 200mg/mL solution, which is the Chinese medicinal extract.

Example 4

A hair conditioner with hair growth function is prepared by:

s1, respectively cleaning polygonum multiflorum, biota orientalis and glossy privet fruit, drying until the moisture content is 5%, crushing and sieving with a 325-mesh sieve to obtain powder, and mixing the polygonum multiflorum powder, the biota orientalis powder and the glossy privet fruit powder according to the mass ratio of 5:2:1, mixing to obtain traditional Chinese medicine powder;

s2, mixing 100g of the traditional Chinese medicine powder obtained in the step S1 with 1500mL of 60wt.% ethanol water solution at a rotating speed of 400r/min, adding 1g of 1-ethyl-3-methylimidazolium tetrafluoroborate, 4g of 1-ethyl-3-methylimidazolium acetate and 2g of benzethonium chloride, heating to 65 ℃, carrying out reflux extraction for 5h, and filtering while the mixture is hot; concentrating the filtrate to 1/6 of the original volume, freeze-drying at-45deg.C for 36h, and dispersing the obtained solid powder in water to obtain 200mg/mL solution, which is the Chinese medicinal extract.

Comparative example 1

A hair conditioner with hair growing function is prepared by the same method as in example 4, and the only difference is that: the preparation of the traditional Chinese medicine extract is not added with 1-ethyl-3-methylimidazolium tetrafluoroborate and 1-ethyl-3-methylimidazole acetate.

Comparative example 2

A hair conditioner with hair growing function is prepared by the same method as in example 1, and the only difference is that: the preparation of the traditional Chinese medicine extract is not added with 1-ethyl-3-methylimidazolium tetrafluoroborate.

Test example 1

The cause of hair loss is very complex and has not been well defined. Oxidative stress is considered to be one of the causes of psychological alopecia. Measuring DPPH clearance is a widely accepted way to evaluate oxidation resistance of substances. The DPPH clearance rate test steps of the traditional Chinese medicine extract used in the invention are as follows:

(1) Dissolving DPPH in absolute ethyl alcohol to prepare a DPPH solution with the concentration of 0.2 mol/L;

(2) Test group: adding 2mL of the traditional Chinese medicine extract and 2mL of DPPH solution into a centrifuge tube at 25 ℃, placing the centrifuge tube in the dark for reaction, sucking the supernatant after 30min, and testing the absorbance at 517 nm; comparison group: adding 2mL of absolute ethyl alcohol and 2mL of DPPH solution into a centrifuge tube at 25 ℃, sucking the supernatant for 30min, and testing absorbance at 517 nm; blank group: adding 2mL of the traditional Chinese medicine extract and 2mL of absolute ethyl alcohol into a centrifuge tube at 25 ℃, sucking the supernatant for 30min, and testing the absorbance at 517 nm;

(3) Calculating the clearance rate

Wherein A is ₀ To test the absorbance of group A ₁ Absorbance, A, of the control group ₂ Absorbance for the blank group;

three parallel samples were arranged in parallel for each group of tests in step (3) above, and the absorbance was averaged.

The results of DPPH and the clearance are shown in Table 1.

Table 1 clearance of DPPH from Chinese medicinal extract

From the test results of comparative examples 1 and 2, it can be seen that benzethonium chloride has a limited effect of improving the DPPH clearance of the compound traditional Chinese medicine extract, and is almost negligible. Examples 1-2 the DPPH clearance of the herbal extracts was significantly improved by adding 1-ethyl-3-methylimidazolium tetrafluoroborate and 1-ethyl-3-methylimidazolium tetrafluoroborate during the extraction of the herbal mixtures, probably because 1-ethyl-3-methylimidazolium tetrafluoroborate and 1-ethyl-3-methylimidazolium tetrafluoroborate enhance the polarity of the active substances in the herbal extracts and increase the extraction efficiency. Example 3 the two ionic liquids on the surface have a certain promoting effect, and the content of effective substances in the traditional Chinese medicine extract is further improved. The DPPH clearance of example 4, to which benzethonium chloride was also added, was significantly enhanced after the addition of both ionic liquids relative to example 3. This is probably because, under the effect of the ionic liquid promoting the extraction of the traditional Chinese medicine, benzethonium chloride is mixed in the system to reduce the dissolution resistance, so that the effective components are easier to dissolve from the traditional Chinese medicine, the content of the effective substances is obviously increased, and the DPPH clearance is obviously enhanced.

An important role in androgenetic alopecia is dihydrotestosterone, which is a metabolite of testosterone. The levels of dihydrotestosterone and the quantity and activity of 5α -reductase in the scalp and serum of androgenic alopecia patients are higher than those of normal people. The inhibition effect on 5 alpha-reductase can be used as an evaluation index of the hair conditioner with alopecia function. In vitro 5 alpha-reductase inhibition test reference journal article "preparation of formulated plant anti-hair loss and hair growth Material and efficacy evaluation study". The results of the inhibition rate of 5 alpha-reductase of the prepared traditional Chinese medicine extract (concentration of 200 mg/L) are shown in Table 2.

TABLE 2 inhibition of 5 alpha-reductase by herbal extracts

Finasteride is a well-known competitive inhibitor of 5α -reductase. From the test results in table 2, it can be seen that the traditional Chinese medicine extract in example 4 of the present invention has a good 5 α -reductase inhibition rate, and the addition of the extract in the hair conditioner can provide the hair conditioner with the hair loss prevention function.

Test example 2

The mouse genome is highly similar to human, the hair growth variation cycle is similar to human, and the hair growth effect of the traditional Chinese medicine extract is simulated by using a mouse model.

Male C57BL mice were randomly grouped: ten mice in the normal group, the model group, the minoxidil group and the traditional Chinese medicine extraction group are subjected to adaptive culture for one week, the back hair of the mice is removed 1 day before the test, testosterone (the daily application amount is 5 mg/kg) is applied to the backs of the mice in the other groups except the normal group, and the mice are continuously applied for 28 days. At the time of molding, the back area of each group of mice is coated with corresponding test liquid: the normal group and the model group are smeared with 0.3mL of physiological saline every day; the minoxidil group was given 0.3mL of minoxidil liniment daily; the Chinese medicine extract group is smeared with 0.3mL of Chinese medicine extract (the concentration is 200 mg/L). The hair and tissue differences of the mice of the different groups were tested after the 28-day test and the results are shown in table 3.

TABLE 3 results of hair and tissue differentiation in mice

As can be seen from the test results of table 3, the traditional Chinese medicine composition of example 4 of the present invention can promote hair growth of mice, significantly increase hair length, hair weight and total number of hair follicles, and the overall effect is similar to that of minoxidil liniment for preventing alopecia. This is probably because the Chinese medicinal composition has good oxidation resistance and 5 alpha-reductase inhibition.

In addition, signs of mice and common mice after the traditional Chinese medicine extract of the invention is applied for 6 months are detected, and the results show that the mice applied with the traditional Chinese medicine extract of the invention are all healthy and have no difference from the common mice and have no abnormal reaction. These results show that the hair conditioner developed based on the traditional Chinese medicine composition has better hair growth function and is suitable for long-term use.

Test example 3

In daily life, people are inevitably exposed to sunlight, and hair is damaged to some extent. The head luminescence aging refers to the change of chemical structure and photodegradation of hair caused by ultraviolet radiation in sunlight, and comprises a series of changes such as protein degradation, amino acid degradation, lipid peroxidation, pigment particle degradation and the like. Referring to the Shuoshi paper, evaluation study of anti-photoaging efficacy of hair care products, the anti-photoaging performance of a hair sample using the conditioner of the present invention was tested by simulating ultraviolet rays with a xenon lamp, and the steps were as follows:

(1) Cutting off natural black hair strands, cutting off hair roots and hair tips, taking hair strands with the middle position of 18cm long, soaking in anhydrous diethyl ether for 24 hours, removing greasy dirt and impurities, flushing with running water for 3 times, smearing 3wt.% of sodium dodecyl sulfate aqueous solution on the hair strands, kneading and foaming for 2 minutes, standing lmin, soaking in water for 30 seconds, flushing the hair strands with water for three times, placing on paper towels, standing at the room temperature of 25 ℃ for 24 hours, and naturally airing;

(2) Dividing the hair bundles obtained in the step (1) into a control group and a test group, wherein the mass of each hair bundle is 10g; the control group did not use any conditioner; the test group uses the hair conditioner prepared by the invention, uses 0.5g of the hair conditioner for every 1 gram of hair bundles, washes the hair bundles with water at 40 ℃ after 5 minutes of use, naturally dries the hair bundles, and uses the hair bundles for ten days continuously;

(3) Placing the hair bundles of the comparison group and the test group obtained in the step (2) at 25 ℃ and a relative humidity of 50 percentAt an irradiation intensity of 550W/m ² Is irradiated under a xenon lamp for 36 hours; the hair tress was tested for lipid peroxidation, work to break, and color difference.

The results are shown in Table 4, wherein the higher the lipid peroxidation degree based on the content of malondialdehyde, the higher the peroxidation degree; lower work to break indicates weaker hair; the smaller the color difference, the smaller the change in appearance of the hair after irradiation.

Table 4 results of photo aging resistance test of conditioner

From the test results shown in table 4, it can be seen that the hair conditioner of example 4 of the present invention has good photo-aging resistance after use, probably because the hair conditioner of the present invention has reasonable formulation of each component, has better oxidation resistance, and can form a stable protective layer on the hair surface, thereby inhibiting damage of ultraviolet rays to hair.

Claims

1. A hair conditioner with hair growing function is characterized in that: the material consists of the following raw materials in parts by weight: 5-10 parts of cationic surfactant, 0.05-0.1 part of thickener, 0.1-0.5 part of cationic conditioner, 3-5 parts of moisturizing grease, 1-2 parts of nutrient substances, 0.05-0.1 part of chelating agent, 0.05-0.1 part of preservative, 0.2-0.3 part of pH regulator, 30-80 parts of water and 10-30 parts of traditional Chinese medicine extract;

s1, respectively cleaning polygonum multiflorum, biota orientalis leaves and glossy privet fruits, drying until the water content is 3-5%, crushing to obtain 300-500 mesh powder, and then mixing to obtain traditional Chinese medicine powder;

s2, mixing the materials according to a feed liquid ratio of 1g: (10-15) mL, stirring and mixing the traditional Chinese medicine powder obtained in the step S1 with 50-70wt% ethanol water solution, adding ionic liquid and benzethonium chloride, heating to 60-70 ℃, carrying out reflux extraction for 2-5 h, and filtering while the mixture is hot; concentrating the filtrate to 1/4-1/6 of the original volume, freeze-drying at-45 to-55 ℃ for 24-36 h to obtain powder, and dispersing the powder in water to obtain a solution with the mass concentration of 200-500 mg/mL, namely the traditional Chinese medicine extract; the ionic liquid is 1-ethyl-3-methylimidazolium tetrafluoroborate and 1-ethyl-3-methylimidazolium tetrafluoroborate in a mass ratio of 1: (2-4) mixing.

2. The hair conditioner with hair growth function according to claim 1, wherein: the mass ratio of the polygonum multiflorum, the biota orientalis and the glossy privet fruit in the traditional Chinese medicine powder in the step S1 is (3-5): (2-4): 1.

3. the hair conditioner with hair growth function according to claim 2, wherein: the mass of the ionic liquid in the step S2 is 0.02-0.05 of that of the traditional Chinese medicine powder.

4. A hair conditioner with hair growth function according to claim 3, wherein: the mass of benzethonium chloride in the step S2 is 1-2wt% of that of the traditional Chinese medicine powder.

5. The method for preparing the hair conditioner with hair growth function according to any one of claims 1 to 4, which is characterized by comprising the following steps:

t1, weighing all raw materials according to a formula;

t3, mixing the moisturizing grease, the thickener and the traditional Chinese medicine extract, heating the system to 60-80 ℃, and stirring for 5-10 min at the rotating speed of 100-300 r/min; then adding the mixture into the system I obtained in the step T2, homogenizing the mixture for 2 to 5 minutes at a temperature of between 60 and 80 ℃ at a speed of between 8000 and 12000r/min to obtain a system II;