CN1100886A - Artificial cultivation method for gastrodia elata blume - Google Patents
Artificial cultivation method for gastrodia elata blume Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The present invention relates to an artificial culture method of gastrodia elata by using symbiosis of armillaria mellea and gastrodia elata. Said method consists of the following steps: two-four seeds of gastrodia elata are planted between two bacterial spawns cut into semicircular shape and cultured in a cylinder container, and then those are placed in a pit whose depth is 25-30 cm, and an strain-inoculated wood stick whose diameter is 9-10 cm and length is 15-25 cm is set upright on above-said material, and an strain-inoculated wood stick whose diameter is 12-15 cm and length is 60-65 cm is set upright along the pit, and then the said material is covered by soil, and those steps are repeated. Said invention can save the required quantity of seed of gastrodia elata, and can raise its yield and shorten its harvest period.
Description
The present invention relates to rhizoma Gastrodiae (Gastrodia elata Blume) and basidiomycetous armillaria mellea (Armillaria mella(Vahl.ex Fr.) Krast that a kind of utilization belongs to the orchid family) the method for symbiotic relation cultivation rhizoma Gastrodiae.
Rhizoma Gastrodiae is special medicinal plant, its stem tuber is rhizoma Gastrodiae (Gastrodiae Rhizoma), terrestrial stem is rhizoma gastrodiae (Gastrodiae Herba), the traditional Chinese medical science is just treated dizzy, headache, quadriplegia, hemiplegia, disfluency, diseases such as children's infantile convulsion with above-mentioned scarce stem before 2000.
Above-mentioned stem tuber is crooked slightly extremely square taper of the circle of pressing to one side, long 5-15cm, wide 2-5cm, thick 1-2cm.The surface is ivory buff to yellowish-brown, and the irregular perpendicular crape Zhe and the root of rotation arrangement are arranged.Quality is hard, and it is yellowish-brown to pitchy, glossy that the one side of shape bending is, similar cutin.
Rhizoma Gastrodiae does not have root and green leaf, and in sexual reproduction and agamic growth history, except that sexual reproduction, its whole physiological function is in order to form stem tuber fully.Grow terrestrial stem (bar) during sexual reproduction.The high 60cm-90cm of terrestrial stem is single upright circle, and two kinds of orange yellow or light greens are arranged.Leaf does not contain chlorophyll, so do not carry out photosynthesis.Seeds of Gastrodia elata and stem tuber must be grown up by means of the mycelia of armillaria mellea or the bacterium Shu Caineng of root shape.This is parasitic type a kind of of mycotrophy.
Below sketch the growth history of rhizoma Gastrodiae.The taper of the seeds of Gastrodia elata side of being becomes crescent, about (length) 0.8 μ m, wide 0.2 μ m.Do not have embryo of silkworms, the general degree that germinates under the nature is 1/1000000th.After the seed skin breaks, the intrusion that the embryo of sprouting is restrainted owing to the armillaria mellea bacterium that infects from the outside forms primary ovum and does, the end that primary ovum is done is because through winter dormancy, its active growth, at this moment the bacterium of armillaria mellea bundle is invaded its cortical tissue, lean on the decomposition of the enzyme of cell to supply with nutriment, form the crescent ovum and do.Primary ovum is done the variable color degeneration when the crescent ovum finishes full growth.Crescent ovum xeromenia is crossed further division again in winter, by the bacterium bundle of decompose invading obtain nutrition form trunk and since pruning repeatedly to form the ovum of secondary dried.Putresce when at the end the crescent ovum is dried the initial season of growth, the dried back of passing the winter of 2 deutovums breaks away from from parent, forms independent plant corpus to spring.2 deutovums are dried to be exactly that the ovum of common rhizoma Gastrodiae is done.Help by armillaria mellea forms the arrow fiber crops with mixed bud (inflorescence bud) under nutritious situation.After the arrow fiber crops pass the winter, germinate, elevate above the soil that the flowers are in blossom puts to spring, solid.The arrow fiber crops were in nutrition and become exhausted state when seed flew upward, corrupt then the decomposition.The time difference that is obtained seed by seed is seldom taken 6 years, if the armillaria mellea undergrowth, then this time also will prolong.
Observe armillaria mellea penetration and do dried cell of ovum afterwards and the variation of mycelia to ovum.Armillaria mellea is invaded the dried epidermal area of ovum, and the forms such as cortical cell of the bottom of invading near mycelia have played variation with structure, and the phenomenon that remarkably hold-up and polyoses grain begin to disappear takes place in cytoplasmic density increase.The vacuole disappears, and mycelia is owing to the hyphal knot that is formed the expansion of tangling by the protoplasm encirclement, and rand disperses gradually, and the limit becomes incomplete mycelia.This incomplete mycelia is delivered to the digestion confluent monolayer cells of the inside that is connected by the hole on the cell wall, forms the lamina of woods beginning.At this moment, with cortical tissue in connection with the rope soft tissue cells that contracts the same form and the variation of structure also take place, volume has become two times, cytoplasmic density and vacuole increase to form and upgrade nuclear.Can think that the transportation of this tissue and material has relation.Mycelia has only one deck digestion confluent monolayer cells after a little while, and the amount of the mycelia of intrusion increases, and several layers digestion confluent monolayer cells then occurred.This digests confluent monolayer cells and forms renewal nuclear, and its vacuole is not broken and absorbed the mycelia of the digestion of doing the elementary step to inhale the mode of drinking, and digests completely then and positive synthetic activity.On the other hand, ovum is done when asexual reproduction, and the year propagation number that ovum is done also can reach tens of times to hundred times.
Armillaria mellea (Armillaria mellea(Vahl.ex Fr.) rast) belongs to Basidiomycetes, new Zoopagales, hemp section (Tricholomataces), parasitic and free (floating life) life of battalion.Armillaria mellea is harmful bacterium to overlapping trees (material), but can not destroy the woods in the growth.The optimum growth temperature of armillaria mellea is 25-26 ℃, and appropriate pH is 5.5-6.0.The initial stage form of armillaria mellea is mycelia, and mycelia assembles big mycelia and is the bacterium bundle.Rhizoma Gastrodiae is distributed in Korea S, China, Japan, all have in the Korea S whole area, the main natural distribution of China in Sichuan, province such as Yunnan, Guizhou.
The domestic Wild gastrodia of Korea S has been in exhausted state now, and its demand sharply increases, yet it cultivates difficulty, and price raises.
After Kusano S. in 1911 makes the symbiotic relation of rhizoma Gastrodiae and armillaria mellea clear,, but also have a lot of problems not come to understand as yet both at home and abroad all in the research of carrying out rhizoma Gastrodiae.
Existing artificial cultivation method is to gather the bacterium bundle near the woods that disconnected wood being arranged under nature the soil, cut suitable branch or juggle little, scrape out dark 18cm, wide 60cm at damp ground, the hole that length is random, scatter the bacterium bundle in the hole, sprinkle branch and fallen leaves again, juggle and bacterium bundle heap are gone into enlarged culture four months.The bacterium of this enlarged culture bundle by heap training method, Gao Peifa and fixedly bacterium training method etc. cultivate, harvest time is long, harvest yield is few, and is ineffective.In addition, Ung-Ho LEE, the Korean Patent Application No. of Sung-Kil YU 81-740 number (notification number 83-1780 number) discloses a kind of cultivation method, this method is with diameter 27-36cm at spring and fall, the log of long 60.9-90.9cm is cut little, with the puncher hole of punching thereon, armillaria mellea in the inoculation, on half shaded place of the slope on the sunny side of height above sea level 300-1500m and acid soil property, dig pit, with the needle leaf with after the broad-leaved leaf mixes with 1: 2 ratio, spread on ground and sprinkle soil again, above-mentioned bone wood (having inoculated the log of bacterium) is done vertical arrangement at interval with 36-45cm, after putting kind of fiber crops (hemp) at interval with 9-18cm in the middle of their then, topped fallen leaves and humus soil.Also have, bone wood done vertically to arrange with certain interval, inoculate the cultivation methods of kind of fiber crops simultaneously at the kind bacterium inoculation position of bone wood, and similarly but to have regulated the method etc. at the interval of planting fiber crops well known.
Yet during with above-mentioned existing method, according to what now known, under kilter, per 100 level grounds (every level ground equals 3.305 square metres) needs 150kg kind fiber crops, and its harvest time was above 2 years.In addition, also has such tendency: after the enlarged culture armillaria mellea, plant the occasion of seed again, must use 2 times labour.Therefore, owing to reasons such as benefits, in fact the enforcement of said method be restricted.
But as according to the present invention, 100 level grounds only need 15kg kind fiber crops, can bring up to harvest yield the rhizoma Gastrodiae of the substandard 320kg of drying regime, use high-quality kind fiber crops a little, can improve its harvest yield and be previous methods several times.
In addition, with the configuration mode of this method, the log amount also can greatly be saved.Also have,, also can save labour's effect owing to inoculate simultaneously.Therefore, the present invention compares with method always, is a kind of cultivation method of further reforming at aspects such as yielding ability and economy.
Rhizoma Gastrodiae artificial culture method of the present invention was made of the following stage: putting into wood sawdust and glucose in the cylindrical plastic container earlier is the medium of main component, the inoculation armillaria mellea; Under 20-27 ℃, make constant temperature culture, make columniform kind of bacterium piece after, be cut into the kind bacterium piece of circular plate type; Long at 60-65cm, leave suitable hole with puncher on the periphery of the log of 12-15cm diameter, by former method inoculation bacterium piece, make beam (transversely arranged, a kind of bone wood); It is long to make 15-25cm again, the breath wood of 9-10cm diameter (vertically arranging a kind of bone wood), and identical with the inoculation method of beam, bacterium is also inoculated in the perforate cave; Underground scrape out the wide 30cm of dark 25-30cm, length and cheat (ridge, ウ ネ) arbitrarily after, discoidal kind of bacterium cut in half along diameter between semicircular kind of bacterium, put into kind of numb 2-4, be placed on the end, hole, it is wooden to swing breath on it; Arrange the above-mentioned beam of adjacency with it along the hole, 2 of planting fiber crops are semicircle to plant bacterium and does endways arrangement in abutting connection with beam with having put into, and puts a beam again and will plant bacterium and be clipped in the middle on the above-mentioned semicircle bacterium next door of planting.By the stage as described above, to the last face covers and goes up about 5-10cm soil continuously.About other many methods to set up, give explanation below.
About people's soil cultivation condition of rhizoma Gastrodiae, only could grow the condition that is fit to that therefore must cause rhizoma Gastrodiae kind fiber crops can grow owing to plant fiber crops with armillaria mellea with the symbiosis of rhizoma Gastrodiae kind fiber crops and armillaria mellea.But armillaria mellea comes compared with rhizoma Gastrodiae, and its growth wants much vigorous.When pathologic intrusion of armillaria mellea took place, it was not decomposed by the cortex of rhizoma Gastrodiae, soaked into (going into) rope axle soft tissue in abutting connection with cortex immediately and produce, and did not form hyphal knot.Therefore, when armillaria mellea stretches along cell wall, under the destructive big situation of pair cell wall, corrupt easily by the tissue of penetration, rhizoma Gastrodiae affects adversely.Therefore the growth of wishing armillaria mellea certainly can be in the state that can fully not supply with nutrient component to rhizoma Gastrodiae generation bad influence, while.
The vegetative period of rhizoma Gastrodiae stem tuber is in the 4-10 month, from March November to next year be resting stage.Growth temperature is 16-26 ℃, and growth stops when surpassing 30 ℃.The moisture of soil is suitable with 50-55%, in order to manage the supply of moisture, repaiies drainage and regulate near the cultivation place.
The cultivation of planting bacterium just can with usual way.Particularly in the present invention, in the cylindrical plastic container of diameter 9-10cm, high 20-25cm, put into sawdust, oil cake powder, worm pupa powder, sucrose, glucose etc. as main component, 121 ℃ of following sterilization treatment are after 25 minutes, and the inoculation armillaria mellea was cultivated about 45 days in 20-26 ℃ constant temperature machine.When using the kind bacterium of in the cylindrical plastic container, cultivating, break above-mentioned plastic containers, obtain columniform kind of bacterium piece, be cut into a plurality of disc kind bacterium pieces of high 4-6cm then, again each discoidal kind of bacterium piece is cut in half, make semicircular kind of bacterium piece.(see figure 3)
The water content of log is suitable for the good growth of bacterium bundle at 50-70%.The kind of operable log has roost, chestnut, thatch Mu, ash, Koryo willow etc.The decision use because roost is ideal in the present invention.Usually log in autumn or make nature winter and receive water.
Be used as (transversely arranged bone wood) log (Fig. 1) of beam, its diameter is 12-14cm, and length is 60-70cm.This length is equivalent to be placed on the kind fiber crops on both sides of beam through cultivate the diameter of the circular rhizoma Gastrodiae piece that forms when results.Therefore, when this length during less than described diameter, the rhizoma Gastrodiae piece is overlapping, hinders the growth of rhizoma Gastrodiae, because supply with nutrition by armillaria mellea problem has taken place also.Otherwise, when this length is oversize, between the rhizoma Gastrodiae piece, having produced the space, this all is very uneconomic at the aspects such as use of milpa area and log.For the bacterium bundle that makes armillaria mellea physically well develops, along the log circumference, from length direction, with the spaced apart 3 row holes of 3 cun (1 cun equals 3.3cm), 4 cun spaced apart 4 row holes, 5 cun spaced apart 5 row holes, inoculate cultured kind of bacterium, it is broken that must be noted that kind of bacterium is not staved this moment.
Breath wood (the bone wood of endways arrangement) (see figure 2) with former carpentery workshop is diameter 9-12cm, high 15-25cm, and the black part of central authorities that ceases wooden cross section is preferably narrow and small, and its outside white portion is preferably broad.When using breath wood, can see that the fast ski-running of the bacterium bundle that is arranged under the breath wood.Can think that this is because be subjected to influence by ceasing wood air fed moisture etc. in ground.In the occasion of wild armillaria mellea, can infer that this situation often can be found in underground under trees only stay its underpart after cutting off the trees.Can after the polyethylene stopper is plugged in the inoculation cave of having inoculated bacterium on the long log, they not transferred to yet, above-mentioned log is cut into certain-length makes breath wood.
Fig. 4 is a method in the past, and with reference to Fig. 4, this method with certain interval inoculation kind of bacterium, goes up rhizoma Gastrodiae kind fiber crops on the next door of bone wood every certain intervals kind (placement) for not using breath wood on bone wood.But this existent method is with respect to harvest yield, and the kind fiber crops that need are many, and this not only increases peasant's financial burden, also has a lot of problems such as growth of sliding and planting fiber crops of kind of bacterium.In order to solve this class problem, the invention provides method to set up a kind of novelty, that improved bone wood and plant the good gastrodin cultivation method of growth that fiber crops were being slided well, planted to bacterium.
According to the present invention, the method to set up of 5 all bacterium, breath wood, beam is arranged, Fig. 5 represents that these are provided with state to Fig. 8.
Method 1:(Fig. 5)
1) in the underground hole that about 30cm is dark, 30cm is wide (ridge) of digging, upper berth, the end is the paper (preferably at underground very fast rotten paper, newspaper is good) of size suitably.
2) the disc kind bacterium of high 4-6cm is cut into 2 half, makes 2 semicircles (3), put into 2-4 in cut-away portions and plant fiber crops (4), be placed on the surface in above-mentioned hole.
3) wood (having inoculated the kind bacterium) (2) of resting is placed on kind of the bacterium.
4) by the horizontal discharging beam (inoculated plant bacterium) (1) of above-mentioned breath wood.
5) repeat 1 once more), 2), 3) and, 4) stage.
1) carry out method 1 1), 20,3), 4) step.
2) facing to beam (1), stand on end and plant 2-4 2 semicircle kind bacterium (3) planting fiber crops as described above, and then by upper cord (1).(planting bacterium is sandwiched between the beam)
3) repeat 1 again), 2), 3) and, 4), 5) and each stage.
Method 3:(Fig. 7)
Carry out method 1 1), 2), 3), 4), suffer (leaning on) and beam (1) and put beam again, the kind bacterium (needn't be cut into two semicircles in this case) (3) of gutless fiber crops is sandwiched in therebetween, repeat this stage then.
Method 4: with reference to Fig. 8
This method with accompanying the endways storing of two semicircle kind bacterium of kind of fiber crops, sets beam for not using the method for breath wood, repeats this two stages again.
Method 5: identical with being provided with of method 1,2,3,4, do not put into kind of fiber crops, set kind of bacterium and bone wood, put into kind of fiber crops after 1 year.
As mentioned above, in the hole, place kind of bacterium and plant fiber crops, bone wooden after, earthing 5-10cm is thick.Plant spring and plant bacterium, plant fiber crops, set bone wood after, gather in the crops once 1 year autumn, later every next year is gathered in the crops autumn.At this moment after the results, can handle especially, directly earthing just can be gathered in the crops as before.Because the immature kind fiber crops of rhizoma Gastrodiae have been dispersed in the soil, they just can be gathered in the crops by 1 year after growing.
According to the present invention, when kind of bacterium piece and bone wood contacts, its end face is faced one another, guess can make like this that kind of bacterium piece is easier to be slided on bone wood.In addition, can think, between the two halves of above-mentioned kind of bacterium piece, put into kind of fiber crops, can have thus when fully supply with nutrition from armillaria mellea the early days of growth of kind of fiber crops, obtain the effect that the bacterial classification piece is being slided.So, needn't apply fertilizer in order to supply with nutrient component (fertilizer, compost), and also without weeding.
＜embodiment＞
Prepare log winter, prepare kind bacterium piece and kind fiber crops, breath wood and beam that 100 level ground dry lands are used 1 year spring.With the degree of depth of 25-30cm, make the wide hole of 30cm, 20cm respectively at interval and the wide hole of 30cm and in the dry land, make the drainage of dark 30-40cm, wide 30cm, carry out 10 dikes again with the interval of 20cm.
In above-mentioned hole, put into 2 semicircle bacterium pieces of planting of planting numb 2-4, put breath wood, then put beam, be covered with the thick soil of about 5-10cm.Draining when having managed rainy season, damaging to plants caused by sudden drop in temperature and shortens resting stage when preventing annual November covers straw 400kg, covers with polyethylene, and gather in the crops 1 year autumn.
* cultivate the place: No. 42,2 li on alms bowl in the south mountain, Chun Chuan prefecture
* area under cultivation: 100 level ground * cultivate the time: April 20 nineteen ninety
* plant numb requirement: 15kg* log requirement: 350
* the 1st results period: on October 20th, 1991
Do numb harvest yield when * gathering in the crops for the 1st time: (dry standard) 70kg, the harvest yield of planting fiber crops: 700kg.
* the 2nd results period: on October 30th, 1992
Do numb harvest yield when * gathering in the crops for the 2nd time: (dry standard) 250kg, do not have kind of Taro Aso and produce.
Past kind of bacterium and to plant the rates of sliding of fiber crops very low, in contrast,,, can see the rate of sliding more than 90% owing to breath wood is set and bacterial classification is set with method of clamping according to the present invention.
Secondly, when being benchmark with 100 level grounds, the past needs expensive kind fiber crops to reach 150kg, only needs to be about its 15kg of 10% by the present invention, and results also shorten to 1 year 6 months to 2 years period.Moreover, planting into kind of fiber crops after the past, the bacterium bundle was slided 1 year, the present invention has also saved the labour owing to can inoculate simultaneously.Again, because the management of carrying out in the cultivation process, nutrient component is not supplied with in cultivation midway, can well-grown yet.So cultivation effect of tool excellence of the present invention.Per 100 level grounds of past need 1000 on log, and the present invention only needs 350, and the usage amount of log is saved to original 1/3, and are very economical, the benefit of tool excellence, thereby have competitiveness.Expert expert can make certain change correction to the present invention as can be seen not exceeding of the present invention will putting under the situation of category.The present invention includes the change of all these classes.
Brief description of drawings.
Fig. 1 is the used beam schematic diagram of the present invention.
Fig. 2 is the schematic diagram of the used breath wood of the present invention.
Fig. 3 is the used kind bacterium piece schematic diagram of the present invention.
Fig. 4 is the cultivation schematic diagram for the past.
Fig. 5 is for pressing the 1st method schematic diagram of the present invention.
Fig. 6 is for pressing the 2nd method schematic diagram of the present invention.
Fig. 7 is the 3rd method schematic diagram by invention.
Fig. 8 is for pressing the 4th method schematic diagram of the present invention.
Among the figure,
1 figure beam, 2 are breath wood, 3 are kind of a bacterium piece,
4 are kind of fiber crops, and 5 are perforation, and 6 are the hole.
Claims (5)
1, a kind of artificial cultivation method of rhizoma Gastrodiae is a symbiotic relation of utilizing armillaria mellea (Armillaria mel-lea kast) and rhizoma Gastrodiae, it is characterized in that,
1) the cylindrical armillaria mellea piece that will in the cylindrical plastic container of diameter 9-10cm, cultivate (plant bacterium piece), be cut into the high disc of 4-6cm after, be divided into 2 half again and make semicircular kind of bacterium piece;
2) with above-mentioned two semicircular kind of bacterium pieces, the mode that faces one another with its section is arranged at 30cm dark, at the bottom of the wide hole of 30cm;
3) between the incision face of above-mentioned semicircle kind bacterium piece, put 2-4 and plant fiber crops (rhizoma Gastrodiae);
4) above-mentioned placed to stand on end on the semicircle kind bacterium piece of planting fiber crops to have inoculated plant breath wood bacterium, diameter 9-10cm, high 15-25cm;
5) in a side of above-mentioned breath wood, place the diameter 12-14cm that has inoculated the kind bacterium, the beam of long 60-70cm, soil in the covering along the hole;
6) according to the varying in size of area under cultivation, repeat above-mentioned 1), 2), 3), 4), 5) step.
2, a kind of artificial cultivation method of rhizoma Gastrodiae is a method of utilizing the symbiotic relation of armillaria mellea and rhizoma Gastrodiae, it is characterized in that,
1) will be after the kind bacterium piece cultivated in the cylindrical plastic container of 9-10cm is cut into the high disc kind bacterium piece of 4-6cm, to be divided into two again at diameter, make the semicircle bacterium piece of planting;
2) between above-mentioned 2 semicircle incision faces of planting the bacterium pieces, put into 2-4 and plant fiber crops, with above-mentioned put plant numb kind bacterium piece (circle) place vertically at 30cm dark, at the bottom of the hole in the hole that 30cm is wide;
3) in a side of above-mentioned kind of bacterium piece, place diameter along the hole and be 12-14cm, long 60-70cm inoculation plant the beam of bacterium, native in the covering;
4), repeat above-mentioned 1 according to the area under cultivation difference), 2), 3) step.
3, the artificial cultivation method of rhizoma Gastrodiae as claimed in claim 1, it is characterized in that, at the end, hole, one side of above-mentioned beam, erect between the incision face of two semicircle kind bacterium pieces and placed 2-4 kind bacterium piece (circle) of planting fiber crops, place new beam on its next door, the kind bacterium piece of having placed above-mentioned kind fiber crops is sandwiched between the beam.
4, the artificial cultivation method of rhizoma Gastrodiae as claimed in claim 3 is characterized in that, in a side of described beam, is provided with the kind bacterium piece of a circle of not putting kind of fiber crops.
5, as the artificial cultivation method of each described rhizoma Gastrodiae of claim 1 to 4, it is characterized in that, between described semicircular kind of bacterium piece, do not put into kind of fiber crops, put into kind of fiber crops after 1 year again.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR17628/93 | 1993-09-03 | ||
| KR1019930017628A KR960003572B1 (en) | 1993-09-03 | 1993-09-03 | Method for cultivating gastrodia elata blume |
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| CN1100886A true CN1100886A (en) | 1995-04-05 |
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| CN94108576A Pending CN1100886A (en) | 1993-09-03 | 1994-08-18 | Artificial cultivation method for gastrodia elata blume |
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| CN (1) | CN1100886A (en) |
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| CN105660143B (en) * | 2016-02-18 | 2018-08-24 | 云南省农业科学院药用植物研究所 | A kind of Rhizoma Gastrodiae implantation methods using the artificial bacteria stick of Rhizoma Gastrodiae symbiosis halimasch |
| CN105794495B (en) * | 2016-03-18 | 2020-01-14 | 中国科学院昆明植物研究所 | Armillaria mellea strain symbiotic with gastrodia elata and application thereof |
| CN113099948A (en) * | 2021-03-29 | 2021-07-13 | 中国中医科学院中药研究所 | Soybean straw culture medium for culturing armillaria mellea and armillaria mellea production method |
-
1993
- 1993-09-03 KR KR1019930017628A patent/KR960003572B1/en not_active IP Right Cessation
-
1994
- 1994-08-18 CN CN94108576A patent/CN1100886A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1064810C (en) * | 1996-02-14 | 2001-04-25 | 北京西城区人民政府西长安街街道办事处 | Box cultivating method for continuously collecting tuber of elevated gastrodia |
| CN101461306B (en) * | 2008-08-09 | 2010-12-15 | 李有荣 | Novel methods of sexual reproduction and breeding of wild rhizoma gastrodiae in Changbaishan area of Jilin province |
| CN105075592A (en) * | 2015-08-05 | 2015-11-25 | 青川县农村产业技术服务中心 | Wild-simulating gastrodia elata viridis and gastrodia elata glauca cultivating technology |
| CN105594434A (en) * | 2016-01-14 | 2016-05-25 | 南漳致远农业科技有限公司 | Method for repeated utilization of armillaria mellea material used in cooperation with gastrodia elata planting |
| CN105594434B (en) * | 2016-01-14 | 2018-05-15 | 南漳致远农业科技有限公司 | A kind of method with the close ring bacterium bacterium material recycling of rhizoma Gastrodiae plantation adapted |
| CN107114093A (en) * | 2017-05-11 | 2017-09-01 | 江口县厚谊科技开发有限责任公司 | A kind of technique for cultivation of Gastrodia elata |
| CN112930478A (en) * | 2019-09-20 | 2021-06-08 | 株式会社理学 | Quantitative analysis method, quantitative analysis program, and fluorescent X-ray analyzer |
| US11585769B2 (en) | 2019-09-20 | 2023-02-21 | Rigaku Corporation | Quantitative analysis method, quantitative analysis program, and X-ray fluorescence spectrometer |
Also Published As
| Publication number | Publication date |
|---|---|
| KR950007636A (en) | 1995-04-15 |
| KR960003572B1 (en) | 1996-03-20 |
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