CN110079517A - A kind of method preparing cell block and the kit for making cell block - Google Patents
A kind of method preparing cell block and the kit for making cell block Download PDFInfo
- Publication number
- CN110079517A CN110079517A CN201910439223.0A CN201910439223A CN110079517A CN 110079517 A CN110079517 A CN 110079517A CN 201910439223 A CN201910439223 A CN 201910439223A CN 110079517 A CN110079517 A CN 110079517A
- Authority
- CN
- China
- Prior art keywords
- cell block
- container
- cell
- sodium alginate
- calcium chloride
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Kit the present invention relates to a kind of method for preparing cell block and for making cell block, wherein the method for preparing cell block enlists the services of the coagulator to gather together as by the loose cell sample for lacking adhesiveness using sodium alginate soln and calcium chloride solution；This method prepares cell block, and process is simple, easy to operate, whole preparation process, not only without heterologous protein ingredient, non-specific coloring when follow-up immunization dyeing is reduced, and can be carried out at normal temperature, without additionally being heated, temperature control etc. operation, avoid influence of the high temperature to cell；In addition, be used to prepare cell block sodium alginate soln and calcium chloride solution it is at low cost, and can form in a short time solid-state after two kinds of solution mixing with long-term preservation, loose cell can be enlisted the services of conglomerate, greatly facilitate the production of cell block.
Description
Technical field
The present invention relates to cell block technical fields, and in particular to a kind of method for preparing cell block and for making cell block
Kit.
Background technique
Cell pathology is mainly used for searching the target cells such as tumour cell in the cell sample of acquirement, to reach pathology
Diagnostic purpose.Cell block technology is an important technology of cell pathology, refers to the pine that will lack adherency using various methods
It dissipates cell and enlists the services of aggregation, formed cell block (allied organization's block), be then dehydrated, embedded, be sliced, and then can go immune thin
A series of subsequent experimentals such as born of the same parents' chemical staining, Molecular Detection, to reach permanent preservation to cell sample, precisely detect and refer to
Lead the purpose of clinical targeted therapy.
Compared with histopathology, cytology samples more convenient, the advantages such as, economic, practical minimally invasive with its, in disease
Reason diagnosis aspect plays irreplaceable role.But cytological samples before are only capable of being made into smear, cannot rich integrating cell block,
Embedded section is carried out, cytology can not be played and sample convenient and fast advantage, greatly limit cytological development.In recent years, with
The exploitation and development of cell block technology become cytology hot spot technology using cytological samples production cell block, agglomerate formation
Cell block can equally be handled with tissue samples, while playing cytology sampling advantage, after can carrying out more
Continuous experiment improves diagnosis accuracy, and cell block sample can be carried out Molecular Detection, for clinical targeted therapy provide according to
According to.
The key of cell block production is how to wrap up loose cell condensation, and in the prior art, the production of cell block is normal
Cell condensation substance has agar, egg white, blood plasma and fibrin ferment, casing (the intestines tunica fibrosa for being derived from chitling) etc., using these
Substance makes cell block, usually comes with some shortcomings: 1, the preparation process of cell block is cumbersome (i.e. so that the blocking mistake of cell condensation
Journey is cumbersome), for example, a kind of cell block preparation method disclosed in Chinese patent CN108303295A, using agar as solidifying
Then Gu agent when in use, needs first by agar since agar is normally solid, the agar drop of molten conditions such as
It after suitable temperature, could be added in cell sample, finally make its natural cooling, the cell block after being solidified；This mistake
Journey, it is not only troublesome in poeration, but also need the temperature of reasonable control agar；
2, time-consuming for the preparation process of cell block；
3, higher cost, usage amount is big, makees cell block for example, by using Thrombin plasma legal system, cost is usually higher；
4, cell block is obtained using biogenic substance embedding cell sample, effect is unstable, and is sliced rear backdrop
Color weight, for example, using the cell block of egg white preparation, easy dyeing in later period use process increases invalid background influence testing result.
Summary of the invention
It is an object of the invention to improve in the prior art, preparation process present in cell block preparation process is cumbersome, consumes
The deficiencies of duration, higher cost, unstable effect, a kind of method for preparing cell block is provided, this method uses sodium alginate
Solution and calcium chloride solution are as the coagulator that the loose cell sample net for catching fish or birds for lacking adhesiveness gathers together.Sodium alginate is molten
Liquid and calcium chloride solution are chemical articles common in the art, nontoxic and cheap, using sodium alginate soln
With calcium chloride solution as cohesion cell sample coagulator, can not only by cell condensation loose in cell sample, package, and
Cell block is formed, and is also had the advantage that
1, the process for preparing cell block is simple and time-consuming short, can not only greatly simplify operation, but also can greatly shorten preparation
The operating time of cell block；
2, whole preparation process, no heterologous protein ingredient, so that the effect stability of cell block, reduces follow-up immunization dyeing
When non-specific coloring；
3, whole preparation process carries out at normal temperature, without additionally being heated, temperature control etc. operation, both can letter
Change operation, and it is possible to prevente effectively from influence of the high temperature to cell；
4, the sodium alginate soln and calcium chloride solution for being used to prepare cell block can be with long-term preservations, and two kinds of solution are mixed
Solid-state can be formed after conjunction in a short time, loose cell can be enlisted the services of conglomerate, be conducive to greatly shorten preparation
Time needed for cell block.
The carry out that the cell block prepared can be convenient it is subsequent dehydration, embedding, slice etc. operation, so as to by cell sample into
The subsequent various detections of row.
To improve in the prior art, prepare that existing process during cell block is cumbersome, the drawbacks such as troublesome in poeration, the application
In, the process of cell block is prepared using sodium alginate soln and calcium chloride solution, is included the following steps:
The sodium alginate soln is added in cell sample step (2), is centrifuged after mixing；Cell sample can be slurry
Membrane cavity hydrops, cervical exfoliated cell, Fine-needle puncture cell etc. usually first will be suitable before sodium alginate soln is added
Cell sample is added in centrifuge tube, and appropriate sodium alginate soln is then added, so that cell sample is filled with sodium alginate soln
It after dividing mixing, is centrifuged, at this point, cell is usually gathered in the bottom of centrifuge tube or close to bottom in mixed solution
At position；
The calcium chloride solution is added into the liquid after centrifugation for step (3), obtains cell block after standing.Directly will
Appropriate calcium chloride solution is added in the mixed solution in step (2) after centrifugation, calcium chloride solution and sodium alginate soln
It can voluntarily be solidified after mixing and standing a period of time, while in the inner by the cell encapsulation in cell sample, to obtain cell
Block.
From aforesaid operations step as it can be seen that the process for preparing cell block using sodium alginate soln and calcium chloride solution is very simple
Singly, conveniently, and time-consuming short.
Since in cell sample collected, contained liquid is more, and also to further decrease sodium alginate soln
With the consumption of calcium chloride solution, reduction prepares the cost of cell block, further includes step (1) in further embodiment, to adopting
The cell sample solution of collection is centrifuged, and gives up supernatant, obtains the cell sample.In the present solution, seaweed is added
Before acid sodium solution, by being centrifuged to cell sample solution, realizes the pretreatment to cell sample solution collected, lead to
The pretreatment is crossed, cell included in cell sample solution can be focused in centrifuge tube, lower part, and can give up
Clear liquid so that the concentration of cell is bigger in cell sample solution, so as to sodium alginate soln required for being effectively reduced and
The amount of calcium chloride solution, not only improving reduces cost, and is conducive to increase the quantity of cell included in cell block, especially suitable
The occasion less for cell sample Solution Cell number (or content).
In preferred scheme, the concentration of the sodium alginate soln is 0.75%~1%.
In preferred scheme, the concentration of the calcium chloride solution is 1%~1.5%.
In preferred scheme, the ratio of the sodium alginate soln and the calcium chloride solution is 1:1.
Preferably, in the step (2), the revolving speed when centrifugation is 5000 revs/min.
Preferably, in the step (2), the time of the centrifugation is 5 minutes.
Preferably, in the step (3), the time of the standing is 10 minutes.
For convenient for easily and fast prepare cell block, and also to solve the packaging of the reagent for making cell block
For topic, this application provides a kind of for making the kit of cell block, including box body, for packing sodium alginate soln
One container and second container for packing calcium chloride solution, the first container and second container are respectively arranged at the box body
It is interior.It, not only can be in order to sodium alginate soln and calcium chloride solution by the way that the first container and second container are arranged in box body
Packaging, storage and transport, and convenient for integrating two kinds of reagents, user is more convenient, quick benefit
The preparation of cell block is completed with this kit.
Be it is easy to produce and reduce cost, in the preferred scheme, the first container and second container can use one
Body structure.
In another preferred scheme, the first container is identical as the structure of second container.
In a kind of preferred embodiment provided by the present application, the first container and second container are respectively included for accommodating sea
The valve protection cap of the cryptomere bottle body and the cryptomere bottle body for closing described accommodation section of solution of sodium alginate and calcium chloride solution, the capsule
Shape bottle body is provided with constriction, and is connected by the constriction with the valve protection cap, and the valve protection cap is laminated structure.The first container
It can preferentially adopt with second container made of plastic, due to the presence of constriction, so that the direction from cryptomere bottle body to valve protection cap, hold
The size of device constantly reduces, and in actual use, user need to only rotate valve protection cap, can easily twist into two parts constriction, thus
Convenient for pouring out the intracorporal reagent of cryptomere bottle.
In the preferred scheme, the upper separate valve protection cap of one end in the first container far from valve protection cap and the second container
One end be connected.The structure for not only contributing to whole container is more compact, and is conducive to the first container and second container one
Molding.
In further embodiment, the first container is connected with second container by a flat interconnecting piece.By setting
Flat interconnecting piece is set, the rigidity of cryptomere bottle body can be substantially reduced, so that cryptomere bottle body more easily becomes under the effect of external force
Shape, so that the reagent in the first container and two containers is discharged in the user that is more convenient for by way of extruding.
Due to during preparing cell block, needing to use centrifuge tube, therefore in further scheme, this kit
It further include centrifuge tube, the centrifuge tube is set in the box body.Device needed for preparing cell block is integrated in this kit
Tool and reagent, the user that is more convenient for complete the preparation of cell block.
Compared with prior art, using a kind of method for preparing cell block provided by the invention and for making cell block
Kit has the advantages that
1, this method, the process for preparing cell block is simple and easy to operate, time-consuming short, not only contributes to simplify operation, mention
High efficiency, and the drawbacks of can effectively solve in the prior art；
2, the whole preparation process of this method, no heterologous protein ingredient, so that the effect stability of cell block, will not influence
Subsequent detection；
3, the whole preparation process of this method can carry out at normal temperature, without additionally being heated, temperature control etc. behaviour
Make；
4, in this method, be used to prepare cell block sodium alginate soln and calcium chloride solution can with long-term preservation, and
Solid-state can be formed in a short time after two kinds of solution mixing, loose cell can be enlisted the services of conglomerate, be conducive to big
Time needed for big shortening prepares cell block.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is a kind of flow chart of the method for preparing cell block provided in the embodiment of the present invention.
Fig. 2 is the first container or second in a kind of kit for making cell block provided in the embodiment of the present invention
The structural schematic diagram of container.
Fig. 3 is the first container and second in a kind of kit for making cell block provided in the embodiment of the present invention
Container be connected as one after structural schematic diagram.
Fig. 4 is the cross-sectional view of Fig. 3.
Fig. 5 is the first container and second in a kind of kit for making cell block provided in the embodiment of the present invention
Container be connected as one after structural schematic diagram.
Fig. 6 is the partial sectional view of the front view of Fig. 5.
Description of symbols in figure
The first container 101, second container 102, cryptomere bottle body 103, constriction 104, valve protection cap 105, interconnecting piece 106.
Specific embodiment
Below in conjunction with attached drawing in the embodiment of the present invention, technical solution in the embodiment of the present invention carries out clear, complete
Ground description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Usually exist
The component of the embodiment of the present invention described and illustrated in attached drawing can be arranged and be designed with a variety of different configurations herein.Cause
This, is not intended to limit claimed invention to the detailed description of the embodiment of the present invention provided in the accompanying drawings below
Range, but it is merely representative of selected embodiment of the invention.Based on the embodiment of the present invention, those skilled in the art are not doing
Every other embodiment obtained under the premise of creative work out, shall fall within the protection scope of the present invention.
Embodiment 1
A kind of method for preparing cell block is present embodiments provided, this method uses sodium alginate soln and calcium chloride solution
The coagulator of (i.e. agglomerated together) of gathering together is enlisted the services of as by the loose cell sample for lacking adhesiveness；Utilize alginic acid
Cell in two kinds of reagent cohesion cell samples of sodium solution and calcium chloride solution, to form cell block；Sodium alginate soln and
Calcium chloride solution is chemical article common in the art, nontoxic and cheap, using sodium alginate soln and chlorine
Change calcium solution as cohesion cell sample coagulator, can not only by cell condensation loose in cell sample, package, and finally
Cell block is formed, and is also had the advantage that
1, the process for preparing cell block is simple and time-consuming short, can not only greatly simplify operation, but also can greatly shorten preparation
The operating time of cell block；
2, whole preparation process, no heterologous protein ingredient, so that the effect stability of cell block, reduces follow-up immunization dyeing
When non-specific coloring；
3, whole preparation process carries out at normal temperature, without additionally being heated, temperature control etc. operation, both can letter
Change operation, and it is possible to prevente effectively from influence of the high temperature to cell；
4, the sodium alginate soln and calcium chloride solution for being used to prepare cell block can be with long-term preservations, and two kinds of solution are mixed
Solid-state can be formed after conjunction in a short time, loose cell can be enlisted the services of conglomerate, be conducive to greatly shorten preparation
Time needed for cell block.
Based on above-mentioned advantage, so that the method provided by the present invention for preparing cell block, can effectively solve existing cell
The problems such as preparation process present in block preparation process is cumbersome, time-consuming, higher cost, unstable effect can especially have
Effect avoids the operations such as heating, strict temperature control required during using agar to prepare cell block, so that prepared by this method
Cell block effect stablize, and the operation such as the subsequent dehydration of the carry out that can be convenient of the cell block prepared, embedding, slice, so as to
Cell sample is subjected to subsequent various detections.
As shown in Figure 1, to improve in the prior art, it is cumbersome, troublesome in poeration etc. to prepare existing process during cell block
Drawback in the application, the process of cell block is prepared using sodium alginate soln and calcium chloride solution, is included the following steps:
The sodium alginate soln is added in cell sample step (2), is centrifuged after mixing；Cell sample can be slurry
Membrane cavity hydrops, cervical exfoliated cell, Fine-needle puncture cell etc. usually first will be suitable before sodium alginate soln is added
Cell sample is added in centrifuge tube, and appropriate sodium alginate soln is then added, so that cell sample is filled with sodium alginate soln
It after dividing mixing, is centrifuged, at this point, cell is usually gathered in the bottom of centrifuge tube or close to bottom in mixed solution
At position, it is more convenient for forming the higher high-quality cell block of cell density in the bottom of centrifuge tube；
The calcium chloride solution is added into the liquid after centrifugation for step (3), obtains cell block after standing.Directly will
Appropriate calcium chloride solution is added in the mixed solution in step (2) after centrifugation, calcium chloride solution and sodium alginate soln
It can voluntarily be solidified after mixing and standing a period of time, while in the inner by the cell encapsulation in cell sample, to obtain cell
Block.
From aforesaid operations step as it can be seen that the process for preparing cell block using sodium alginate soln and calcium chloride solution is very simple
Singly, conveniently, and time-consuming short.
Since in cell sample collected, contained liquid is more, and also to further decrease sodium alginate soln
With the consumption of calcium chloride solution, reduction prepares the cost of cell block, further includes step (1) in further embodiment, to adopting
The cell sample solution of collection is centrifuged, and gives up supernatant, obtains the cell sample.In the present solution, seaweed is added
Before acid sodium solution, by being centrifuged to cell sample solution, realizes the pretreatment to cell sample solution collected, lead to
The pretreatment is crossed, cell included in cell sample solution can be focused in centrifuge tube, lower part, and can give up
Clear liquid so that the concentration of cell is bigger in cell sample solution, so as to sodium alginate soln required for being effectively reduced and
The amount of calcium chloride solution, not only improving reduces cost, and is conducive to increase the quantity of cell included in cell block, especially suitable
The occasion less for cell sample Solution Cell number (or content).In the preferred scheme, the sodium alginate soln
Concentration can be 0.75%~1%.
In the preferred scheme, the concentration of the calcium chloride solution can be 1%~1.5%.
In preferred scheme, the ratio of the sodium alginate soln and the calcium chloride solution can be 1:1 or so；It can be with
Understand, the proportionate relationship between two kinds of reagents includes but is not limited to 1:1.
As an example, in the preferred scheme, in the step (2), the revolving speed when centrifugation is that 5000 rpms are left
It is right；It is appreciated that revolving speed when centrifugation includes but is not limited to this revolving speed.
As an example, in the preferred scheme, in the step (2), the time of the centrifugation is 5 minutes or so；It can be with
Understand, time when centrifugation includes but is not limited to 5 minutes.
In the preferred scheme, in the step (3), the time of the standing is 10 minutes or so, because according to experiment
Show that the solidification of cell sample can be realized in the time for usually standing 10 minutes or so, to obtain cell block；It is actually using
In the process, it can according to need the time for being appropriately extended and standing, such as stand 15 minutes, which is not described herein again.
Embodiment 2
For convenient for easily and fast prepare cell block, and also to solve the packaging of the reagent for making cell block
For topic, this application provides a kind of for making the kit of cell block, including box body, for packing sodium alginate soln
One container and second container for packing calcium chloride solution, the first container and second container are respectively arranged at the box body
It is interior.It, not only can be in order to sodium alginate soln and calcium chloride solution by the way that the first container and second container are arranged in box body
Packaging, storage and transport, and convenient for integrating two kinds of reagents, user is more convenient, quick benefit
The preparation of cell block is completed with this kit.
In the present embodiment, box body is made of flexible plastic material, can also be made of hard paper material matter.
In another preferred scheme, the first container is identical as the structure of second container.
As an example, in a kind of preferred embodiment provided by the present application, the first container and second container are respectively included
For accommodating the cryptomere bottle body of sodium alginate soln and calcium chloride solution and the bottle of the cryptomere bottle body for closing described accommodation section
Cap, the cryptomere bottle body is provided with constriction, and is connected by the constriction with the valve protection cap, and the valve protection cap is sheet knot
Structure is held, as shown in Figure 2 convenient for user；The first container and second container can preferentially adopt it is made of plastic, due to constriction
Presence so that the direction from cryptomere bottle body to valve protection cap, the size of container constantly reduces, and in actual use, user only needs
Valve protection cap is rotated, at this point, since there are the mutation in structure between constriction and valve protection cap, so that the junction of constriction and valve protection cap is deposited
It is concentrated in biggish stress, is conducive to user and slightly firmly turns, can easily twist into two parts the connection of constriction and valve protection cap,
Consequently facilitating pouring out the intracorporal reagent of cryptomere bottle.It is appreciated that in the present solution, in the first container and second container respectively
Accommodate the dosage for being used to prepare one or many cell blocks.Be it is easy to produce and reduce cost, it is in the preferred scheme, described
The first container and second container can use integral structure；As an example, as shown in Figures 3 and 4, in another kind in preferred side
In case, one end in the first container far from valve protection cap is connected with one end of the upper separate valve protection cap of the second container；I.e. at this
In scheme, the first container and the structure for not only contributing to whole container are more compact, and are conducive to the first container and the
Two containers are integrally formed.It is appreciated that being used to prepare once in the present solution, being accommodated respectively in the first container and second container
Multiple integrally formed the first containers and the can be arranged in kit for convenient for repeatedly preparing cell block in the dosage of cell block
Two containers, which is not described herein again.
It in the preferred scheme, can be on the cryptomere bottle body of the first container and second container in order to distinguish two kinds of reagents
Separator is respectively set, separator can be text, figure, color etc., so that user can start tries two kinds
Agent distinguishes.
The first container 101 or second container 102 is discharged in the reagent in container when to be easy to use, in further side
In case, the first container 101 is connected with second container 102 by a flat interconnecting piece 106, as shown in Figures 5 and 6, is led to
It crosses and flat interconnecting piece 106 is set, the rigidity of cryptomere bottle body 103 can be substantially reduced, so that cryptomere bottle body 103 is easier outside
It is deformed under the action of power, to be conducive to user by squeezing the reagent in discharge the first container and two containers.
It is appreciated that the material of the interconnecting piece 106 can be identical as the material of the first container 101 and second container 102.
As an example, it is contained in the first container 101 and second container 102, for carrying out the seaweed of a preparation process
The amount of acid sodium solution and calcium chloride solution can be respectively 3~10ml.
Due to needing to use centrifuge tube during preparing cell block, therefore provided by the present embodiment further
Scheme in, this kit further includes centrifuge tube, and the centrifuge tube is set in the box body.System is integrated in this kit
Instrument needed for detailed mass and reagent, the user that is more convenient for complete the preparation of cell block.
The centrifuge tube can be centrifuge tube commonly used in the prior art, be also possible to dedicated for production cell block from
Heart pipe, centrifuge tube as provided by Chinese patent CN108303295A etc., which is not described herein again.
Embodiment 3
As an example, in a kind of kit for making cell block that the present embodiment 3 provides, box body is by flexible plastic
Polybag is made in material, and box body is provided with a first container, a second container and a centrifuge tube, wherein first holds
Device is connected as one with second container, as shown in Figures 5 and 6, the sodium alginate soln of 5ml, alginic acid is housed in the first container
The concentration of sodium solution is 0.75%, and the calcium chloride solution of 5ml is housed in second container, and the concentration of calcium chloride solution is 1%；For
The cell sample solution of cell block is prepared using dropsy of serous cavity, is prepared into the dropsy of serous cavity using this kit
Detailed process is as follows for cell block:
The first, box body is opened, centrifuge tube is taken out, appropriate dropsy of serous cavity is added in centrifuge tube, in 5000 rpms
Revolving speed under be centrifuged 5 minutes, after the completion of being centrifuged, give up supernatant；
The second, the first container is taken out from box body, unscrews the valve protection cap of the first container, the sodium alginate in the first container is molten
Liquid is added in centrifuge tube, and shakes centrifuge tube, so that sodium alginate soln is sufficiently mixed with the cell sample in centrifuge tube, so
It is centrifuged 5 minutes under the revolving speed of 5000 rpms afterwards；
Third, the valve protection cap for unscrewing second container, the calcium chloride solution in the first container is added in centrifuge tube, is stood big
About after ten minutes, it is seen that the mixing liquid in centrifuge tube solidifies completely, it is hereby achieved that required cell block.
Known to by the above process, on the one hand, prepare cell block using method provided by the present embodiment and kit, lead to
It often only needs 10 minutes or so, required time far smaller than prepares cell using agar, egg white, blood plasma or casing etc. in the prior art
It is time needed for block, especially opposite using for agar, it usually needs to be heated up and the operation that cools down, wherein only to heat up
10 minutes or so time just usually is needed, therefore method provided by the present embodiment and kit is used to prepare cell block, it can be with
Easily and efficiently complete the preparation of cell block；On the other hand, by above-mentioned practical preparation flow it is found that using this method and examination
When agent box prepares cell block, without carrying out any heating operation, not only it is possible to prevente effectively from influence of the high temperature to cell, Er Qieke
Effectively to avoid during preparing cell block using agar, stringent and harsh heating and controlled temperature conditions；In addition, passing through we
Method and the cell block of kit preparation, effect stability are conducive to the subsequent detection to cell.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.
Claims (10)
1. a kind of method for preparing cell block, which is characterized in that will be lacked using sodium alginate soln and calcium chloride solution conduct
The loose cell sample of adhesiveness enlists the services of the coagulator to gather together.
2. the method according to claim 1 for preparing cell block, which comprises the steps of:
The sodium alginate soln is added in cell sample step (2), is centrifuged after mixing；
The calcium chloride solution is added into the liquid after centrifugation for step (3), obtains cell block after standing.
3. the method according to claim 2 for preparing cell block, which is characterized in that further include step (1), to the thin of acquisition
Born of the same parents' sample solution is centrifuged, and gives up supernatant, obtains the cell sample.
4. the method according to claim 2 for preparing cell block, which is characterized in that the concentration of the sodium alginate soln is
0.75%~1%.
5. the method according to claim 2 for preparing cell block, which is characterized in that the concentration of the calcium chloride solution is
1%~1.5%.
6. the method according to claim 2 for preparing cell block, which is characterized in that the sodium alginate soln and the chlorine
The ratio for changing calcium solution is 1:1.
7. a kind of for making the kit of cell block, which is characterized in that including box body, for packing sodium alginate soln
One container and second container for packing calcium chloride solution, the first container and second container are respectively arranged at the box body
It is interior.
8. according to claim 7 for making the kit of cell block, which is characterized in that the first container and second
Container respectively includes cryptomere bottle body for accommodating sodium alginate soln and calcium chloride solution and for closing described accommodation section
The valve protection cap of cryptomere bottle body, the cryptomere bottle body is provided with constriction, and is connected by the constriction with the valve protection cap, the bottle
Cap is laminated structure.
9. according to claim 8 for making the kit of cell block, which is characterized in that separate in the first container
One end of valve protection cap is connected with one end of the upper separate valve protection cap of the second container.
10. according to claim 9 for making the kit of cell block, which is characterized in that the first container and the
Two containers are connected by a flat interconnecting piece.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910439223.0A CN110079517A (en) | 2019-05-24 | 2019-05-24 | A kind of method preparing cell block and the kit for making cell block |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910439223.0A CN110079517A (en) | 2019-05-24 | 2019-05-24 | A kind of method preparing cell block and the kit for making cell block |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110079517A true CN110079517A (en) | 2019-08-02 |
Family
ID=67421739
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910439223.0A Pending CN110079517A (en) | 2019-05-24 | 2019-05-24 | A kind of method preparing cell block and the kit for making cell block |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110079517A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT202000014863A1 (en) * | 2020-06-22 | 2021-12-22 | Hospitex Int S R L | KIT FOR THE PREPARATION OF CYTOINCLUSIONS |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5213845A (en) * | 1991-10-07 | 1993-05-25 | Armstrong World Industries, Inc. | Coloring process |
| CN102198022A (en) * | 2011-05-23 | 2011-09-28 | 西安交通大学 | Solid forming method of active cell-hydrogel organ structure |
| CN102344905A (en) * | 2010-08-04 | 2012-02-08 | 北京大学 | Bionic culture model for chondrocyte, and preparation method thereof |
| KR20130084799A (en) * | 2012-01-18 | 2013-07-26 | 주승연 | Composition and kit for preparing a cell block, and method for preparing cell blocks using the same |
| CN108956221A (en) * | 2018-04-10 | 2018-12-07 | 上海交通大学附属第人民医院松江分院 | A method of pathology film-making is carried out using sodium alginate |
-
2019
- 2019-05-24 CN CN201910439223.0A patent/CN110079517A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5213845A (en) * | 1991-10-07 | 1993-05-25 | Armstrong World Industries, Inc. | Coloring process |
| CN102344905A (en) * | 2010-08-04 | 2012-02-08 | 北京大学 | Bionic culture model for chondrocyte, and preparation method thereof |
| CN102198022A (en) * | 2011-05-23 | 2011-09-28 | 西安交通大学 | Solid forming method of active cell-hydrogel organ structure |
| KR20130084799A (en) * | 2012-01-18 | 2013-07-26 | 주승연 | Composition and kit for preparing a cell block, and method for preparing cell blocks using the same |
| CN108956221A (en) * | 2018-04-10 | 2018-12-07 | 上海交通大学附属第人民医院松江分院 | A method of pathology film-making is carried out using sodium alginate |
Non-Patent Citations (1)
| Title |
|---|
| 刘斌: "细胞培养", 北京/西安：世界图书出版公司, pages: 86 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT202000014863A1 (en) * | 2020-06-22 | 2021-12-22 | Hospitex Int S R L | KIT FOR THE PREPARATION OF CYTOINCLUSIONS |
| EP3929559A1 (en) * | 2020-06-22 | 2021-12-29 | Hospitex International S.r.l. | Method and kit for the preparation of cellblocks |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN206523325U (en) | A kind of saliva collection save set | |
| US20150338321A1 (en) | Cell pathology tubes and associated cell processing methods | |
| CN110079517A (en) | A kind of method preparing cell block and the kit for making cell block | |
| CN107782575A (en) | A kind of system and method collected and detect analyte in sample | |
| CN201859075U (en) | Novel disposable excrement sampler | |
| CN206204332U (en) | Cell is centrifuged blake bottle | |
| CN2790415Y (en) | Filter type sperm separator | |
| CN208171631U (en) | Sample device and its package body | |
| CN201139556Y (en) | Sampler | |
| CN205042500U (en) | Cell centrifuging tube | |
| CN203609925U (en) | Closed platelet concentrator | |
| CN202497449U (en) | Three-combination bag used for collection, separation and preservation of umbilical cord blood | |
| CN206804314U (en) | excrement sampling bottle and excrement sampling system | |
| CN102242054A (en) | Totally-enclosed cell and cell factor preparation device and preparation method thereof | |
| CN202173399U (en) | Blood collector capable of directly separating plasma | |
| CN104383713A (en) | Extraction method for medical tea active components | |
| CN206986177U (en) | A kind of integrated purification of nucleic acid extractor | |
| CN108192813A (en) | A kind of trace dna elutes collection device | |
| CN206945339U (en) | A kind of sample collection device | |
| CN106148174A (en) | Platelet rich plasma and mononuclearcell collection tube and suit thereof | |
| CN204601195U (en) | Bag for collecting cord blood | |
| CN203798612U (en) | Excrement sample collection device | |
| CN203458633U (en) | Device for preparing platelet rich plasma | |
| CN106582909A (en) | Centrifuge tube and method for preparing platelet-rich plasma by using same | |
| CN207685292U (en) | The gene detection system independently detected can be achieved |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |