CN106148174A - Platelet rich plasma and mononuclearcell collection tube and suit thereof - Google Patents
Platelet rich plasma and mononuclearcell collection tube and suit thereof Download PDFInfo
- Publication number
- CN106148174A CN106148174A CN201510111137.9A CN201510111137A CN106148174A CN 106148174 A CN106148174 A CN 106148174A CN 201510111137 A CN201510111137 A CN 201510111137A CN 106148174 A CN106148174 A CN 106148174A
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- Prior art keywords
- mononuclearcell
- filled
- collection tube
- rich plasma
- separation gel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/02—Blood transfusion apparatus
- A61M1/029—Separating blood components present in distinct layers in a container, not otherwise provided for
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Abstract
The present invention is a kind of platelet rich plasma and mononuclearcell collection tube, including former pipe, it is filled with cell separation liquid in former pipe, it is filled with separation gel I above cell separation liquid, the above buffer solution that is filled with of separation gel I, the above separation gel II that is filled with of buffer solution, the above of separation gel II is filled with anti-coagulants, being sealed in above-mentioned each composition in former pipe by plug, the outside of former pipe is provided with label；Described platelet rich plasma and mononuclearcell collection tube suit, including kit, being provided with resettlement groove in kit, PRP and mononuclearcell collection tube, syringe, blood taking needle, lyophilized thrombin, calcium chloride, entry needle and alcohol swab are sequentially arranged on resettlement groove in corresponding groove.The present invention can once centrifuge i.e. separable results PRP and mononuclearcell；After centrifugal, PRP and mononuclearcell position are determined, it is easy to extraction；One suit can complete whole PRP and mononuclearcell extraction, simplifies operating procedure, beneficially clinical practice, reduces operational pollution.
Description
Technical field
The present invention is a kind of blood collection tube and suit thereof, particularly a kind of platelet rich plasma and
Mononuclearcell collection tube and suit thereof.
Background technology
Platelet rich plasma and mononuclearcell are the important component in blood.
(English name is Platelet Rich Plasma to platelet rich plasma, and it is abbreviated as
PRP) there is hemostasis, the function of pain relieving rapidly, so PRP is used primarily for wound in war and repaiies
Multiple.The nineties in last century, Switzerland physician finds, PRP not only has very strong hemostasis energy
Power, under fixing concentration and certain pH value, additionally it is possible to produce needed for a large amount of healthy skin
Growth factor, growth factor has very strong reparation expanding capacity to human body.Due to PRP tool
There is hemostasis and pain-relieving and repair expanding capacity, so being widely used in beauty treatment, orthopedics and traumatology, shaping
The numerous areas such as surgery, the department of stomatology.But due to PRP gather need to meet fixing concentration and
Certain pH value, so requiring very high to acquisition technique, causes PRP expensive, it is difficult to
Push clinic widely to.
Mononuclearcell mainly includes lymphocyte and monocyte, and its medium size lymphocyte includes T
Lymphocyte and bone-marrow-derived lymphocyte；Granulocyte includes neutrophil cell, eosinophil and basophilic
Property granulocyte, they mainly with human immunity response and regulate and control closely related, be body defenses
Important component part.The collection of traditional mononuclearcell, first gathers peripheral blood,
It is then passed through the dilution of corresponding solution, then carry out gradient centrifugation with cell separation liquid, finally
Obtaining mononuclearcell, whole gatherer process, step is various, open-sky technique, efficiency
Lowly, it is inconvenient for.
Collection to PRP and mononuclearcell, is all to gather respectively, and uses opening at present
Formula operates, and there is PRP and mononuclearcell easily, preparation method low by outside contamination, the rate of recovery
Unstable and easy by defects such as experimental implementation are affected.Again because PRP and mononuclearcell proportion are
Having scope, being difficult to determine the position of PRP and mononuclearcell after centrifuging, extracted amount is difficult to really
Fixed, cause concentration inaccurate and affect experimental data and result for the treatment of.It is experimentally confirmed that in PRP
Blood platelet can be separated by centrifugal force with the mononuclearcell in blood plasma.After PRP extracts,
Need to mix with fibrin ferment and/or calcium chloride, stimulate PRP to produce bioactie agent, more can
Play repair function.
Content of the invention
The collection that the invention aims to overcome existing PRP and mononuclearcell is easily got dirty
Dye, the rate of recovery is low, acquisition method is unstable and centrifugal after be difficult to determine PRP and mononuclearcell
The defect of position, invents a kind of collection by PRP and mononuclearcell and integrates and will adopt
Collection utensil concentrates on the collection tube of one platelet rich plasma convenient to operate and mononuclearcell
And suit.
It is an object of the invention to realize as follows: described platelet rich plasma and list
Individual nucleus collection tube, including former pipe, is filled with cell separation liquid, cell separation liquid in former pipe
Being filled with separation gel I above, the above buffer solution that is filled with of separation gel I, above buffer solution
Being filled with separation gel II, the above of separation gel II is filled with anti-coagulants, by plug by above-mentioned
Each composition is sealed in former pipe, and the outside of former pipe is provided with label.
Described platelet rich plasma and mononuclearcell collection tube can may not be used with predetermined vacuum yet
Predetermined vacuum.
Described cell separation liquid is lymphocyte separation medium, leucocyte separation liquid or mononuclearcell
Separating liquid, its proportion is 1.077 ± 0.005；Separation gel I is serum separation gel, its proportion
For 1.079-1.091；Buffer solution is HANK ' S buffer solution, PBS or citrate
Buffer solution；Separation gel II is serum separation gel, and its proportion is 1.025-1.050；Anti-coagulants
For edta salt, citrate, heparinate, oxalates, hirudin or blood maintenance liquid one
Plant or combination.
Described former pipe is to make of glass or plastic material, and plastics include PET, PEN or PNT；
Plug is to make of halogenated butyl rubber；Plug can be substituted by aluminum plastic film.
Described platelet rich plasma and mononuclearcell collection tube suit, including kit, suit
It is provided with resettlement groove in box, PRP and mononuclearcell collection tube, syringe, blood taking needle, lyophilized
Fibrin ferment, calcium chloride, entry needle and alcohol swab are sequentially arranged on resettlement groove in corresponding groove.
Described syringe is female Luer syringe；Blood taking needle is butterfly blood taking needle or straight blood sampling
Pin.
The positive effect of the present invention is as follows: 1) can once centrifuge i.e. separable results PRP and list
Individual nucleus.2) after centrifuging, PRP and mononuclearcell position are determined, it is easy to extraction；3)
One suit can complete whole PRP and mononuclearcell extraction, simplifies operating procedure, favorably
In clinical practice, reduce operational pollution.
Brief description
Fig. 1 is the collection tube structure chart of platelet rich plasma and mononuclearcell
Fig. 2 is collection tube sleeve structure figure
In figure: 1 former pipe 2 plug 3 anti-coagulants
4 separation gel II 5 buffer solution 6 separation gel I
7 cell separation liquid 8 label 9 kits
10 resettlement groove 11 heparin tube 12 syringes
13 blood taking needle 14 lyophilized thrombin 15 calcium chloride
16 entry needle 17 alcohol swabs
Detailed description of the invention
As it is shown in figure 1, described platelet rich plasma and mononuclearcell collection tube, including former pipe
1, it is filled with cell separation liquid 7 in former pipe 1, above cell separation liquid 7, be filled with separation gel I
The above buffer solution 5 that is filled with of 6, separation gel I 6, the above of buffer solution 5 is filled with separation
The above of glue II 4, separation gel II 4 is filled with anti-coagulants 3, by plug 2 by above-mentioned respectively
Composition is sealed in former pipe 1, and the outside of former pipe 1 is provided with label 8.
Described cell separation liquid 7 is that lymphocyte separation medium, leucocyte separation liquid or single core are thin
Born of the same parents separate liquid, and its proportion is 1.077 ± 0.005；Separation gel I 6 is serum separation gel, its
Proportion is 1.079-1.091；Buffer solution 5 is HANK ' S buffer solution, PBS or lemon
Lemon phthalate buffer；Separation gel II 4 is serum separation gel, and its proportion is 1.025-1.055；
Anti-coagulants 3 is that edta salt, citrate, heparinate, oxalates, hirudin or blood are protected
The one of nutrient solution or combination.
Described former pipe 1 is to make of glass or plastic material, plastics include PET or PEN or
PNT；Plug 2 is to make of halogenated butyl rubber；Plug 2 can be substituted by aluminum plastic film.
As in figure 2 it is shown, platelet rich plasma and mononuclearcell collection tube suit, including suit
Box 9, is provided with resettlement groove 10 in kit 9, the 11st, PRP and mononuclearcell collection tube are injected
Device the 12nd, blood taking needle the 13rd, lyophilized thrombin the 14th, calcium chloride the 15th, entry needle 16 and wine
Essence cotton 17 is sequentially arranged on resettlement groove 10 in corresponding groove.
Described syringe 12 is female Luer syringe；Blood taking needle 13 is butterfly blood taking needle or straight
Formula blood taking needle.
During use, first extraction blood, can centrifuge after blood to be extracted, after centrifuging, in pipe
Component positions change because red blood cell proportion is maximum, which is 1.09-1.092, institute
Be positioned at bottom collection tube after centrifugal, separation gel I proportion between 1.079-1.090, so
It is positioned on red blood cell.Cell separation liquor ratio is heavily 1.077 ± 0.005, is positioned at separation gel I
On, and mononuclearcell proportion is 1.075-1.09, is positioned among cell separation liquid, blood
The proportion of platelet is 1.03-1.035, is positioned on mononuclearcell, and separation gel II proportion is
1.021-1.030, is positioned on blood platelet, and blood plasma mean specific gravity is about 1.02, is positioned at and adopts
Collector is topmost.Due to centrifugal action, PRP and mononuclearcell is made to be fixed on separation gel I
It and between separation gel II, so is easy to extract.
Claims (5)
1. platelet rich plasma and a mononuclearcell collection tube, including former pipe, its feature exists
In: it is filled with cell separation liquid in former pipe, above cell separation liquid, be filled with separation gel I, point
From the above buffer solution that is filled with of glue I, the above separation gel II that is filled with of buffer solution, separate
The above of glue II is filled with anti-coagulants, is sealed in above-mentioned each composition in former pipe by plug,
The outside of former pipe is provided with label.
2. platelet rich plasma according to claim 1 and mononuclearcell collection tube,
It is characterized in that: cell separation liquid is that lymphocyte separation medium, leucocyte separation liquid or monokaryon are thin
Born of the same parents separate liquid, and its proportion is 1.077 ± 0.005；Separation gel I is serum separation gel, its ratio
It is heavily 1.079-1.090；Buffer solution is HANK ' S buffer solution, PBS or citric acid
Salt buffer；Separation gel II is serum separation gel, and its proportion is 1.025-1.050；Anti-freezing
Agent is edta salt, citrate, heparinate, oxalates, hirudin or blood maintenance liquid
One or combination.
3. platelet rich plasma according to claim 1 and mononuclearcell collection tube, its
It is characterised by: described former pipe is to make of glass or plastic material, and plastics include PET, PEN
Or PNT；Plug is to make of halogenated butyl rubber；Plug can be substituted by aluminum plastic film.
4. a platelet rich plasma and mononuclearcell collection tube suit, it is characterised in that: bag
Include kit, in kit, be provided with resettlement groove, PRP and mononuclearcell collection tube, injection
Device, blood taking needle, lyophilized thrombin, calcium chloride, entry needle and alcohol swab are sequentially arranged in resettlement groove
In upper corresponding groove.
5. platelet rich plasma according to claim 4 and mononuclearcell gather pipe box
Dress, it is characterised in that: described syringe is female Luer syringe；Blood taking needle is butterfly blood sampling
Pin or straight blood taking needle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510111137.9A CN106148174A (en) | 2015-03-13 | 2015-03-13 | Platelet rich plasma and mononuclearcell collection tube and suit thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510111137.9A CN106148174A (en) | 2015-03-13 | 2015-03-13 | Platelet rich plasma and mononuclearcell collection tube and suit thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106148174A true CN106148174A (en) | 2016-11-23 |
Family
ID=58063457
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510111137.9A Pending CN106148174A (en) | 2015-03-13 | 2015-03-13 | Platelet rich plasma and mononuclearcell collection tube and suit thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106148174A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107022475A (en) * | 2016-02-02 | 2017-08-08 | 付士明 | Chromosome karyotype analysis sample evacuated collection container |
| CN107617236A (en) * | 2017-09-28 | 2018-01-23 | 安徽信灵检验医学科技有限公司 | A kind of platelet rich liquid |
| CN111468204A (en) * | 2020-06-08 | 2020-07-31 | 珠海朗泰生物科技有限公司 | Platelet-rich plasma preparation tube with controllable components and preparation method thereof |
Citations (7)
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|---|---|---|---|---|
| US20110263408A1 (en) * | 2008-11-07 | 2011-10-27 | Kunihiro Suto | Blood serum or blood plasma separation method |
| CN102321582A (en) * | 2011-08-29 | 2012-01-18 | 深圳市中美康士生物科技有限公司 | Granulocyte separating medium, and method for granulocyte separating and activity detecting |
| US20120053041A1 (en) * | 2009-10-28 | 2012-03-01 | Glotech Co., Ltd. | Centrifugal separation kit and methods for centrifugal separation using the same |
| CN202430219U (en) * | 2011-11-23 | 2012-09-12 | 付士明 | Vacuum blood collection tube for mononuclear cell |
| CN202430220U (en) * | 2011-12-05 | 2012-09-12 | 付士明 | Vacuum blood collecting tube for preplaced Percoll separating solution |
| CN202426528U (en) * | 2011-11-23 | 2012-09-12 | 付士明 | Platelet rich plasma (PRP) rapid extraction vacuum blood collection tube |
| CN202860558U (en) * | 2012-07-23 | 2013-04-10 | 郑成云 | Kit for rapidly separating platelet rich plasma |
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2015
- 2015-03-13 CN CN201510111137.9A patent/CN106148174A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110263408A1 (en) * | 2008-11-07 | 2011-10-27 | Kunihiro Suto | Blood serum or blood plasma separation method |
| US20120053041A1 (en) * | 2009-10-28 | 2012-03-01 | Glotech Co., Ltd. | Centrifugal separation kit and methods for centrifugal separation using the same |
| CN102321582A (en) * | 2011-08-29 | 2012-01-18 | 深圳市中美康士生物科技有限公司 | Granulocyte separating medium, and method for granulocyte separating and activity detecting |
| CN202430219U (en) * | 2011-11-23 | 2012-09-12 | 付士明 | Vacuum blood collection tube for mononuclear cell |
| CN202426528U (en) * | 2011-11-23 | 2012-09-12 | 付士明 | Platelet rich plasma (PRP) rapid extraction vacuum blood collection tube |
| CN202430220U (en) * | 2011-12-05 | 2012-09-12 | 付士明 | Vacuum blood collecting tube for preplaced Percoll separating solution |
| CN202860558U (en) * | 2012-07-23 | 2013-04-10 | 郑成云 | Kit for rapidly separating platelet rich plasma |
Non-Patent Citations (2)
| Title |
|---|
| 张利萍等: "《化工新型材料》", 28 February 2013 * |
| 董忠生等: "《临床基础检验》", 31 August 2012, 华中科技大学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107022475A (en) * | 2016-02-02 | 2017-08-08 | 付士明 | Chromosome karyotype analysis sample evacuated collection container |
| CN107617236A (en) * | 2017-09-28 | 2018-01-23 | 安徽信灵检验医学科技有限公司 | A kind of platelet rich liquid |
| CN111468204A (en) * | 2020-06-08 | 2020-07-31 | 珠海朗泰生物科技有限公司 | Platelet-rich plasma preparation tube with controllable components and preparation method thereof |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20161123 |
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| WD01 | Invention patent application deemed withdrawn after publication |