CN110066738B

CN110066738B - AM fungal inoculant and application method thereof

Info

Publication number: CN110066738B
Application number: CN201910263414.6A
Authority: CN
Inventors: 魏文君; 王勇; 张瑾; 董志新; 苏贞峰
Original assignee: Elion Ecological Restoration Co ltd; Elion Resources Group Co Ltd
Current assignee: Elion Ecological Restoration Co ltd; Elion Resources Group Co Ltd
Priority date: 2019-04-02
Filing date: 2019-04-02
Publication date: 2020-10-23
Anticipated expiration: 2039-04-02
Also published as: CN110066738A

Abstract

The application provides an AM fungal inoculant and an application method thereof, and relates to the technical field of mine restoration. An AM fungal inoculant comprises an AM fungal culture medium and a host plant root system, wherein the AM fungal culture medium comprises a strain culture medium and AM fungi, and the strain culture medium comprises the following components in parts by weight: 20-50 parts of zeolite, 5-30 parts of vermiculite, 5-30 parts of river sand and 2-5 parts of water-retaining agent. The application method of the AM fungal inoculant comprises the following steps: s1, sequentially adding a mine matrix modifier, mine soil and an AM fungal inoculant into the planting pits; s2 transplanting the seedlings into the planting pits, adding the seedlings into the mine soil again, and carrying out AM fungal dip-dyeing planting. The method can ensure the adaptability of the propagated strains in the semiarid mining area, and effectively improve the activity and spore yield of the strains after the microbial inoculum is used; meanwhile, the nutrient and moisture conditions of the mining area land can be obviously improved, the biomass of the vegetation is improved, and the recovery rate and the quality of the mining area vegetation are accelerated.

Description

AM fungal inoculant and application method thereof

Technical Field

The application relates to the technical field of mine restoration, in particular to an AM fungal inoculant and an application method thereof.

Background

The development and utilization of mineral resources make great contribution to the high-speed development of economy and urbanization construction of China, but extensive over-development of the mineral resources also causes a series of environmental problems and seriously damages the local ecological environment. The sand-dust weather and water and soil loss are caused, and natural geological disasters such as landslide and collapse are caused, so that the ecological system is degraded and even collapsed, and the sustainable development and the regional ecological safety of surrounding areas are seriously threatened. Restoring and rebuilding a degraded mine ecosystem is at an urgent need.

The vegetation recovery is an important link of mine ecological restoration, not only plays a role in constructing initial vegetation for waste land, but also can improve soil structure, nutrient condition and microbial environment and promote the recovery of the structure and function of the whole ecological system. The soil in mining areas is mostly disturbed, damaged by mechanical excavation or transportation, has poor physical, chemical and biological activity, damages to the soil structure, poor soil fertility, insufficient water, loss of productivity, rapid deterioration of the growth environment of organisms and microorganisms, and difficult survival of vegetation or difficult closing of vegetation after survival to form forests. The AM fungus is used as a strain which can establish a symbiotic relationship with a plant root system, can help plants to absorb water and nutrients, particularly phosphorus and trace elements, enhance the disease resistance, stress resistance and drought resistance of the plants, reduce the loss of nitrogen and phosphorus and improve the resistance of the plants to heavy metal pollution; the AM fungus has great application potential in the field of mine restoration.

Most of the presently disclosed methods for the propagation and application of the AM fungus are not directed to the mine. Patent application No. CN00132468.3 entitled "culture of arbuscular mycorrhizal fungi with glass beads as culture medium" provides a specific culture device and culture medium; the patent application with the application number of CN02159722.7 and the name of 'method for producing arbuscular mycorrhizal fungi agent' is that a plurality of host plants are sowed and mixed on a mixed culture substrate to produce AM fungi and the like; the patent application document with the application number of CN201610990849.7 and the name of 'a propagation method of arbuscular mycorrhizal fungi microbial inoculum in coal mine' mainly adds weathered coal into a culture medium, and the medium is easy to obtain. However, these methods for culturing AM fungi cannot guarantee the fungi to exert their effects to the maximum extent under the severe survival conditions in the mining area, and are more suitable for the application in a single type of mining area, and the application range is limited.

Disclosure of Invention

In view of this, the embodiment of the present application provides an AM fungal inoculant and an application method thereof, so as to solve technical defects in the prior art.

An AM fungal inoculant comprises an AM fungal culture medium and a host plant root system, wherein the AM fungal culture medium comprises a strain culture medium and AM fungi, and the strain culture medium comprises the following components in parts by weight: 20-50 parts of zeolite, 5-30 parts of vermiculite, 5-30 parts of river sand and 2-5 parts of water-retaining agent.

Further, the host plant root system comprises any two or three of corn, sorghum, alfalfa, white clover, clover and plantain.

Further, the AM fungus is rhizospora rhizogenes.

Further, the water-retaining agent comprises any one of an inorganic water-retaining agent sodium bentonite, an organic water-retaining agent sodium polyacrylate, potassium polyacrylate, starch grafted acrylate and a composite water-retaining agent.

An application method of the AM fungal inoculant comprises the following steps:

s1, sequentially adding a mine matrix modifier, mine soil and an AM fungal inoculant into a planting pit;

and S2, transplanting the nursery stocks into the planting pits, adding the nursery stocks into the mine soil again, and carrying out AM fungal dip-dyeing planting.

Further, the mine matrix modifier in the step S1 comprises the following components in parts by weight: 5-10 parts of a water-retaining agent, 15-40 parts of bentonite, 20-40 parts of coal cinder and 15-30 parts of animal waste.

Further, the animal manure comprises one or more of pig manure, cow manure, sheep manure, chicken manure, duck manure and goose manure.

Further, the step S1 includes:

adding a mine matrix modifier into the planting pits to 1/10-1/6 of the depth of the planting pits;

adding mine soil into the planting pits, wherein the addition amount of the mine soil is the same as that of the mine matrix modifier;

and (3) spreading an AM fungal inoculant in the planting pit, wherein the total height of the mine matrix improver, the mine soil and the AM fungal inoculant is 1/3 of the depth of the planting pit.

Compared with the prior art, the method has the following advantages:

1. the adaptability of the propagated strains in the semi-arid mining area is ensured, and the activity and spore yield of the strains after the microbial inoculum is used can be effectively improved;

2. after the mine matrix modifier is added and the AM fungi are inoculated, the nutrient and moisture conditions of the mining area land can be obviously improved, the biomass of the vegetation is improved, and the recovery rate and the quality of the mining area vegetation are accelerated;

3. the method is suitable for various mining areas and has wide application range.

Drawings

FIG. 1 is a schematic diagram of the steps of the AM fungal inoculant application method of the present application.

Detailed Description

The following is a description of specific embodiments of the present application.

The AM fungus in the application is a soil fungus, can establish a symbiotic relationship with more than 80% of land plant roots to form arbuscular mycorrhiza, and can promote the roots to absorb mineral nutrition of soil, improve the water metabolism condition of plants, improve the resistance/tolerance of the plants to heavy metal stress, enhance the disease resistance of the plants and the like after the AM fungus and the plants form the mycorrhiza.

The zeolite in the present application is a generic term for minerals of the zeolite group, and is an alkali or alkaline earth metal aluminosilicate mineral containing water, and the zeolite has ion exchange properties, adsorptive separation properties, catalytic properties, stability, chemical reactivity, reversible dehydration properties, electrical conductivity, and the like.

The vermiculite in the application is a natural, inorganic and nontoxic mineral substance, can expand under the action of high temperature, and belongs to silicate.

The river sand in the application is produced by long-time repeated collision and friction of natural stones under the natural state through the acting force of water, and the river sand is a non-metal ore with more complex components, certain smoothness on the surface and more impurity content.

The water-retaining agent in the application uses super absorbent resin, is a functional polymer material with extremely strong water absorption capacity, is non-toxic and harmless, and releases and absorbs water repeatedly, so people compare the water-retaining agent with a 'micro reservoir' in agriculture. Meanwhile, the fertilizer can absorb fertilizer and pesticide and slowly release the fertilizer and the pesticide, so that the fertilizer efficiency and the pesticide effect are improved.

The corn in the application is an annual herbaceous plant of Zea of Gramineae, is an important food crop and feed crop, is also a crop with the highest total yield all over the world, and contains abundant protein, fat, vitamins, trace elements, cellulose and the like.

The sorghum in the application is a gramineous annual herbaceous plant which is pleased with warm and light and has certain high-temperature resistance.

The alfalfa in the application is a generic name of alfalfa plants, is commonly called as burclover and is a perennial herbaceous plant. The alfalfa is various in types, is mostly a wild herbaceous plant, is similar to clover, is drought-resistant, cold-resistant and heat-resistant, has high yield and high quality, and can improve soil.

The white clover, the axanthus, the Holland seesaw and the like are named in the application, and the perennial herb plants have wide adaptability, strong heat and cold resistance, can grow vigorously in acid soil and sandy soil and also have certain ornamental value. A large number of root nodules are grown on the main root and the lateral roots of the white clover, can fix nitrogen in the air, and only a small amount of phosphorus-potassium fertilizer is needed to be applied in the planting process without applying nitrogen fertilizer, so that the branches and leaves are luxuriant.

The clover in the application is a general name of various herbaceous plants with three finger-shaped compound leaves, and mainly comprises three types: some species of Trifolium Pratense L of Leguminosae, Medicago, Oxalidaceae, etc. The clover can increase soil organic matters, is favorable for fertilizing soil, can reduce the temperature of soil in summer, stabilizes the air humidity of an orchard, is favorable for the growth of fruit trees in summer, and can also keep a good soil structure.

Plantago asiatica in this application is a biennial or perennial herb. The plantain herb has strong adaptability, cold resistance, drought resistance, low requirement on soil and good growth on warm, moist, sunny and sandy fertile soil.

The bentonite in the application is a non-metal mineral product with montmorillonite as a main mineral component. The bentonite can be sodium bentonite (alkaline soil), calcium bentonite (alkaline soil), and natural bleaching earth (acid soil or acid clay), wherein the calcium bentonite comprises calcium sodium base and calcium magnesium base. The bentonite has strong hygroscopicity and expansibility, can adsorb water with volume being 8-15 times of that of the bentonite, and has volume expansion being several times to 30 times; can be dispersed into a gelatinous state and a suspended state in an aqueous medium, and the medium solution has certain viscosity, thixotropy and lubricity; has stronger cation exchange capacity; the adsorption capacity for various gases, liquids and organic substances is certain, and the maximum adsorption capacity can reach 5 times of the self weight; the admixture of the high-performance water-based adhesive and water, mud or fine sand has plasticity and cohesiveness; surface active acidic bleaching earths are capable of adsorbing colored ions.

The coal cinder in the application is one of industrial solid wastes, and the main components are silicon dioxide, aluminum oxide, ferric oxide, calcium oxide, magnesium oxide and the like.

Animal waste, also commonly referred to as feces, is the large intestine waste of animals, one quarter of the feces is water, and the rest is mostly protein, inorganic matter, fat, undigested dietary fiber, dehydrated digestive juice residue, cells and dead bacteria shed from intestinal tracts, as well as vitamin K and vitamin B.

Example 1

Further, host plant roots include roots of plants that are capable of establishing a symbiotic relationship with the AM fungus. The host plant root system comprises any two or three of corn, sorghum, alfalfa, white clover, clover and plantain.

Further, the AM fungus is Rhizopus aphagus intraradics rhizospora.

The culture method of the AM fungal inoculant comprises the following steps:

(1) mixing zeolite, vermiculite, river sand and water-retaining agent uniformly according to a certain proportion, and preparing into strain culture medium, wherein the strain culture medium is used before use⁶⁰Performing Co gamma ray irradiation sterilization;

(2) selecting suitable host plant seeds, and using 10% H₂O₂Soaking for 15min, washing with deionized water until completely washed, then germinating on sterilized filter paper, and accelerating germination in dark at 25 deg.C;

(3) adding the sterilized strain culture medium into a pot culture, putting a plurality of host plant seeds after germination and pregermination, scattering a pure strain Rhizopagus intraradices root cystospora purchased from the market, adding deionized water according to 15% of the total mass of the put components, covering the upper layer of the seeds with about 1cm of the strain culture medium, watering according to the condition, and watering the seeds once every two weeks;

(4) culturing for 4-6 months, and collecting the cultured AM fungal culture medium and the host plant root system as the AM fungal inoculant obtained by propagation.

The water-retaining agent that adds of this application innovation in the bacterial culture medium, although the water-retaining agent is insoluble in water, can absorb the water that is equivalent self weight one hundred times to can effectively restrain the evaporation of water, after the infiltration water-retaining agent in soil, can restrain the evaporation of moisture at to a great extent, thereby improve the saturated water content of soil, reduce the saturated hydraulic conductivity of soil, thereby slow down the speed of soil release water, reduce the infiltration and the loss of soil moisture, reach the purpose of moisturizing.

Meanwhile, the water-retaining agent can also stimulate the growth and development of crop roots, so that the length of the roots is increased, the number of the roots is increased, and the roots can keep good growth under a drought condition. The water-retaining agent has the functions of absorbing and preserving water, so that nutrient substances required by the growth of crops such as soluble fertilizers, pesticides and the like can be fixed in the water-retaining agent, the loss of the soluble nutrients is reduced to a certain extent, the utilization rate of the fertilizers, the pesticides and the like is improved, and the growth condition of the crops is improved.

The water-retaining agent has good heat preservation performance, and after the water-retaining agent is used, the heat energy generated by partial daytime illumination can be kept by utilizing absorbed water to adjust the nighttime temperature, so that the day and night temperature difference of soil is reduced.

The water-retaining agent can also improve the soil structure, and the water-retaining agent is applied to the soil, and can change the soil around from compact to loose and increase the pores along with the regular change of water absorption expansion and water loss shrinkage, thereby improving the permeability of the soil to a certain extent.

Example 2

An application method of the AM fungal inoculant is shown in figure 1, and comprises the following steps:

Further, adding a mine matrix modifier into the planting pits to 1/10-1/6 of the depth of the planting pits;

The water-retaining agent is added into the mine matrix modifier in the application in the same way, the mine matrix modifier and the AM fungal inoculant are sequentially added into the planting pit, the double water-retaining agent can play a double water-retaining role, and double guarantee can be provided for the AM fungi to play a role in promoting the plant root system to absorb nutrient components and the like.

Furthermore, the water-retaining agent can also play a synergistic effect with the AM fungal inoculant, and the water-retaining agent can effectively improve the soil environment, ensure the water content in the soil and reduce the permeation and loss of soil moisture, so that the loss of nutrient components in the soil is reduced, and the effect of the AM fungal inoculant on the soil and the root system of the nursery stock is further enhanced.

Furthermore, the existence of the water-retaining agent can effectively enlarge the application range of the application, so that the AM fungal inoculant is no longer only suitable for a single type of mining area, and can ensure that the action is exerted to the maximum extent under the severe living conditions of the mining area, the biomass of the vegetation is improved, and the recovery rate and the quality of the vegetation in the mining area are accelerated.

Test example 1

In the test example, a treatment group and a control group were simultaneously set, wherein the treatment group was treated by the following method:

(1) preparing a strain culture medium, which comprises the following components in parts by weight: 45 parts of zeolite, 25 parts of vermiculite, 25 parts of river sand and 5 parts of water-retaining agent which are uniformly mixed, wherein the water-retaining agent selected in the embodiment is potassium polyacrylate, and the strain culture medium is used before use⁶⁰Performing Co gamma ray irradiation sterilization;

selecting corn seed and alfalfa seed as host plant seed, and using 10% H₂O₂Soaking for 15min, washing with deionized water for 8 times to completely clean, and filtering in sterile conditionGerminating on paper, and accelerating germination at 25 deg.C in dark.

Adding the sterilized AM fungus culture medium to 2/3 parts of a potted plant, putting 4 germinated corn seeds and 15 alfalfa seeds, scattering a pure strain Rhizopus guinsitraradics arbuscular mycorrhizal fungus purchased from the market, adding deionized water according to 15 percent of the total mass of the put components, covering the upper layer of the seeds with about 1cm of the fungus culture medium, pouring water once every 3 to 5 days according to the drought condition during the period, and pouring Hoagland nutrient solution with the pH of 5.5 to 6.0 once every two weeks (as shown in Table 1);

TABLE 1 ingredient table for Hoagland nutrient solution

Note: the rightmost column shows the mother liquor (ml) required for the preparation of 1L of nutrient solution.

(2) Culturing for 5 months, and collecting the cultured AM fungal culture medium and the host plant root system as the AM fungal inoculum obtained by propagation.

(3) And (3) carrying out AM fungal infection planting on the transplanted seedling of the sabina vulgaris in the semiarid mining area.

Digging a planting pit according to the size of the root system of the sapphia aristata seedling, wherein the size is 50cm (diameter) by 40cm (height), firstly applying a mine matrix modifier to a position 4cm above the bottom surface of the planting pit, then applying mine soil with the same amount as the mine matrix modifier, then scattering an AM fungal agent to a position 1/3 of the depth of the planting pit, transplanting the sapphia aristata seedling with the root system soaked by a water-retaining agent of 20-30 cm into the planting pit, backfilling the mine soil, treading the soil with feet, watering for one time to permeate water, and supplementing and applying the AM fungal agent again after 5 months.

The mine matrix modifier adopted in the embodiment comprises the following components in parts by weight: 5 parts of potassium polyacrylate, 30 parts of bentonite, 40 parts of coal cinder and 25 parts of goose manure.

The control group adopts the traditional vegetation recovery method, only the common mine matrix modifier is added in the treatment process, the AM fungal inoculant is not added, and other steps and conditions are the same as those of the treatment group.

The plant row spacing between the juniperus chinensis seedlings is 20cm x 30 cm.

After the sabina vulgaris is transplanted for one year, the impregnation condition and the growth condition of the root system of the sabina vulgaris are detected, and the data shown in table 2 are obtained.

TABLE 2. infection frequency and infection intensity of Selaginella moellendorfii hieron root systems under different treatments

It can be seen from table 2 that the impregnation frequency and the impregnation intensity of the root system of sabina vulgaris in the treated group are significantly higher than those of the control group, that is, by adopting the novel mine vegetation restoration method of the present invention, the impregnation frequency and the intensity of the root system of sabina vulgaris are significantly higher than those of the traditional method, which indicates that the AM fungal inoculant applied in the treatment process impregnates the root system of sabina vulgaris and forms a reciprocal symbiont, while a small amount of infection exists in the traditional method without the AM fungal inoculant, because the native AM fungi exist in the mine land, but the number of fungi is small.

The method is characterized in that sabina vulgaris grows for about one year, the survival rate and the growth situation of the sabina vulgaris are counted, and the survival rate of the sabina vulgaris planted by the traditional method is relatively low and is only about 50% -65%, and leaves are withered and yellow; the survival rate of the sabina vulgaris planted by the method is remarkably improved to more than 95%, and the sabina vulgaris grows well and has luxuriant branches and leaves. Therefore, the AM fungus strain propagated by the invention has strong multiplication capacity, the hypha outside the root can quickly establish a symbiotic relationship with the root system of sabina vulgaris, can better adapt to the environment of a mine, improve the survival rate of nursery stocks, and simultaneously, the AM fungus strain can improve the nutrient and moisture conditions of the soil in a mining area together with the applied water retention agent and mine matrix modifier, improve the biomass of vegetation and be beneficial to the recovery of the vegetation in the mining area.

Test example 2

(1) preparing a strain culture medium, which comprises the following components in parts by weight: 40 parts of zeolite, 30 parts of vermiculite, 27 parts of river sand and 3 parts of water-retaining agent, wherein the water-retaining agent used in the embodiment is sodium bentonite, and the strain culture medium is used before use⁶⁰Performing Co gamma ray irradiation sterilization;

selecting corn seed and plantain seed as host plant seed, and using 10% H₂O₂Soaking for 15min, washing with deionized water for 8 times until completely washed, then germinating on sterilized filter paper, and accelerating germination in dark condition at 25 deg.C.

Adding a sterilized AM fungus culture medium to 2/3 positions of a potted plant, adding 4 germinated corn seeds and 15 plantain seeds, scattering a pure strain Rhizopus guinsitraradics arbuscular mycorrhizal fungus purchased from the market, adding deionized water according to 15% of the total mass of the added components, covering the upper layer of the seeds with about 1cm of the strain culture medium, pouring water once every 3-5 days according to drought conditions, pouring Hoagland nutrient solution with the pH of 5.5-6.0 once every two weeks, and preparing the Hoagland nutrient solution as shown in Table 1 in example 1.

The mine matrix modifier adopted in the embodiment comprises the following components in parts by weight: 7 parts of sodium bentonite, 33 parts of bentonite, 30 parts of coal cinder and 30 parts of duck manure.

After the sabina vulgaris is transplanted for one year, the impregnation condition and the growth condition of the root system of the sabina vulgaris are detected, and the data shown in table 3 are obtained.

TABLE 3 root infection frequency and infection intensity of sabina vulgaris under different treatments

As can be seen from Table 3, after the sabina vulgaris is transplanted for one year, the dip-dyeing frequency and the dip-dyeing strength of the treatment group are obviously higher than those of the control group, the dip-dyeing frequency and the dip-dyeing strength of the root system of the sabina vulgaris can reach more than 85% by adopting the method of the invention, while the dip-dyeing frequency and the dip-dyeing strength of the root system of the sabina vulgaris are only about 10% by adopting the traditional method, which indicates that the applied AM fungal inoculant can form a mutual and beneficial symbiont with the root system of the sabina vulgaris.

Meanwhile, through statistics of the survival number and the growth condition of sabina vulgaris, the survival rate of the sabina vulgaris planted by the method is over 95%, the growth condition is good, and the survival rate of the sabina vulgaris planted by the traditional method is relatively low and is only about 60%. Therefore, the method is more beneficial to the survival of the mine vegetation and the recovery of the mine soil.

Test example 3

(1) preparing a strain culture medium, which comprises the following components in parts by weight: 50 parts of zeolite, 25 parts of vermiculite and 20 parts of river sand5 parts of water-retaining agent, and the water-retaining agent selected in the embodiment is sodium polyacrylate, and the strain culture medium is used before use⁶⁰Performing Co gamma ray irradiation sterilization;

selecting sorghum seeds and alfalfa seeds as host plant seeds, and using 10% H₂O₂Soaking for 15min, washing with deionized water for 8 times until completely washed, then germinating on sterilized filter paper, and accelerating germination in dark condition at 25 deg.C.

Adding a sterilized AM fungus culture medium to 2/3 parts of a potted plant, adding 4 germinated sorghum seeds and 15 alfalfa seeds, scattering a pure strain Rhizopus guinsitraradics arbuscular mycorrhizal fungus purchased from the market, adding deionized water according to 15% of the total mass of the added components, covering the upper layer of the seeds with about 1cm of the strain culture medium, pouring water once every 3-5 days according to the drought condition during the period, pouring Hoagland nutrient solution with the pH of 5.5-6.0 once every two weeks, and preparing the Hoagland nutrient solution as shown in Table 1 in example 1.

(2) Culturing for 6 months, and collecting the cultured AM fungal culture medium and the host plant root system as the AM fungal inoculum obtained by propagation.

The mine matrix modifier adopted in the embodiment comprises the following components in parts by weight: 10 parts of sodium polyacrylate, 30 parts of bentonite, 20 parts of coal cinder and 40 parts of chicken manure.

After the sabina vulgaris is transplanted for one year, the impregnation condition and the growth condition of the root system of the sabina vulgaris are detected, and the data shown in table 4 are obtained.

TABLE 4 root infection frequency and infection intensity of sabina vulgaris under different treatments

The result trend is the same as the test example 1 and the test example 2, and after the sabina vulgaris is transplanted for one year, the effect achieved by the method is obviously better than that achieved by the traditional method. As shown in Table 4, the treated sabina vulgaris by the method of the present invention has an AM fungal infection frequency of 90% or more and an infection intensity of 85% or more, and the death rate of sabina vulgaris is even less than 5%. The control group planted by the traditional method depends on the indigenous AM fungi, the infection frequency of the root system of the sabina vulgaris is about 14 percent, the infection intensity is maintained at about 10 percent, the plants of the sabina vulgaris grow badly, and the survival rate is about 60 percent. The comparison of the two mine vegetation recovery modes fully shows that the applied AM fungal inoculant can establish a good reciprocal and profitable symbiotic relationship with the root system of sabina vulgaris, obviously improves the nutrient and moisture conditions of the land in a mining area together with a mine matrix modifier, improves the survival rate and biomass of vegetation, and has great significance for the recovery of the vegetation in the mining area.

In this document, "first", "second", and the like are used only for distinguishing one from another, and do not indicate the degree and order of importance, the premise that each other exists, and the like.

In this context, "equal", "same", etc. are not strictly mathematical and/or geometric limitations, but also include tolerances as would be understood by a person skilled in the art and allowed for manufacturing or use, etc.

Unless otherwise indicated, numerical ranges herein include not only the entire range within its two endpoints, but also several sub-ranges subsumed therein.

While the preferred embodiments and examples of the present application have been described in detail, the present application is not limited to the embodiments and examples described above, and various changes can be made without departing from the spirit of the present application within the knowledge of those skilled in the art.

Claims

1. An application method of an AM fungal inoculant is characterized by comprising the following steps:

s1, adding a mine matrix modifier, mine soil and an AM fungal inoculant into a planting pit in sequence, wherein the mine matrix modifier comprises: adding a mine matrix modifier into the planting pits to 1/10-1/6 of the depth of the planting pits; adding mine soil into the planting pits, wherein the addition amount of the mine soil is the same as that of the mine matrix modifier; spreading an AM fungal inoculant into the planting pits, wherein the total height of the mine matrix improver, the mine soil and the AM fungal inoculant is 1/3 of the depth of the planting pits;

s2, transplanting the nursery stocks into the planting pits, adding the nursery stocks into the mine soil again, and carrying out AM fungal dip-dyeing planting;

wherein the AM fungal inoculant comprises an AM fungal culture medium and a host plant root system, and the AM fungi are rhizospora intraradicans (Rhizophagus intraradics);

the AM fungus culture medium comprises a strain culture medium and AM fungus, wherein the strain culture medium comprises the following components in parts by weight: 20-50 parts of zeolite, 5-30 parts of vermiculite, 5-30 parts of river sand and 2-5 parts of water-retaining agent;

the mine matrix modifier comprises the following components in parts by weight: 5-10 parts of a water-retaining agent, 15-40 parts of bentonite, 20-40 parts of coal cinder and 15-30 parts of animal manure;

the water-retaining agent in the AM fungus culture medium or the mine matrix modifier is any one of an inorganic water-retaining agent sodium bentonite, an organic water-retaining agent sodium polyacrylate, a polyacrylic acid potassium, a starch grafted acrylate and a composite water-retaining agent.

2. The method of claim 1, wherein the host plant root system comprises any two or three of corn, sorghum, alfalfa, white clover, and plantain.

3. The application method of the AM fungal agent according to claim 1, wherein the animal manure comprises one or more of pig manure, cow manure, sheep manure, chicken manure, duck manure and goose manure.