CN110029046A - A kind of experimental provision that establishing explosion injury cell model and experimental method - Google Patents
A kind of experimental provision that establishing explosion injury cell model and experimental method Download PDFInfo
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- 208000013883 Blast injury Diseases 0.000 title claims abstract description 36
- 238000002474 experimental method Methods 0.000 title claims abstract description 23
- 239000000725 suspension Substances 0.000 claims abstract description 25
- 238000004113 cell culture Methods 0.000 claims abstract description 22
- 238000011160 research Methods 0.000 claims abstract description 11
- 238000004880 explosion Methods 0.000 claims description 16
- 239000010963 304 stainless steel Substances 0.000 claims description 9
- 229910000589 SAE 304 stainless steel Inorganic materials 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 9
- 229920006267 polyester film Polymers 0.000 claims description 9
- 230000035699 permeability Effects 0.000 claims description 8
- 238000005474 detonation Methods 0.000 claims description 6
- 239000002390 adhesive tape Substances 0.000 claims description 3
- 239000006143 cell culture medium Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 238000003466 welding Methods 0.000 claims 1
- 230000002349 favourable effect Effects 0.000 abstract description 3
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- 230000006378 damage Effects 0.000 description 5
- 230000004796 pathophysiological change Effects 0.000 description 3
- 230000035939 shock Effects 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000008499 blood brain barrier function Effects 0.000 description 1
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- 239000002360 explosive Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The present invention relates to a kind of experimental provision for establishing explosion injury cell model and experimental methods, including hanging ring, lifting rope, suspension hook, hanger, quick-fried source rope, cell culture board mount and tissue culture plate.The hanging ring is connected by lifting rope with suspension hook, the suspension hook is welded on hanger, the hanger includes three outer ring stand, middle frame and interior ring stand circular supports, and up and down four be connected and fixed bar, the quick-fried source rope is fixed on hanging ring, the cell culture board mount includes support rod, fixed frame, butterfly screws and matching nut, and the tissue culture plate pedestal upper end is welded on hanger, and lower end is connected and fixed tissue culture plate.The invention also discloses the experimental methods of the experimental provision.Compared with prior art, experimental provision production is simple, and primary experiment can carry out three different blast impulse pressure groups, and the research of every group of eight samples is easy to operate；The explosion injury cell model of foundation, accuracy is high, and controllability is strong, favorable repeatability.
Description
Technical field
The present invention relates to cell model constructing technology field more particularly to a kind of experiment dresses for establishing explosion injury cell model
It sets and experimental method.
Background technique
In recent years, explosion injury becomes the hot spot of military medical research.It refers to the high temperature and high pressure gas pressure generated after explosion
Contracting surrounding medium forms the shock wave propagated in a radial manner outward from explosive source, acts on each organ and system of human body,
Cause different degrees of and classification damage.Air burst shock wave pressure, which changes, to be indicated with Friedlander equation, i.e. wink
Between raised superpressure, subatmospheric power is then decayed to exponential form, is terminated when being balanced with environmental pressure, mesolow
Phase duration is about twice of superpressure phase duration.Explosion wave transfers energy on its propagation path
The degree of injury of human body, human body depends on following five factors: first is that initial peak overpressure；Second is that the duration of superpressure；
Third is that the medium that explosion wave is propagated；Fourth is that object is at a distance from quick-fried source；Fifth is that the enlarge-effect of opposite confined space.When quick-fried
Fried shock wave can not only cause the physical damnification of primary when human body, but also can lead to intracorporal tissue and thin
A series of pathophysiological change can occur for born of the same parents, such as oxidative stress, inflammatory reaction, the destruction of blood-brain barrier and nerve dysfunction
Deng the damage of body is further aggravated.
All the time for the experimental study of explosion injury, animal model is mainly utilized, clear explosion wave is passed through
Physical parameter, with the scene that physics level reconstruction explodes, simulated explosion is damaged caused by body.However body after explosion injury
Reaction is a sufficiently complex pathophysiological process, and many factors can influence the generation and development of damage, at this time using dynamic
Object model can not accurately the cascade reaction between further research and analysis cell and disclose histiocytic after explosion injury
Pathophysiological change, it is therefore desirable to explosion injury is studied and inquired into from cellular elements level.But it is there is no at present dedicated for building
The experimental provision of vertical explosion injury cell model, this brings inconvenience to the research of explosion injury cellular level.In order to more preferable
Ground study explosion injury mechanism and pathophysiological change, provide foundation for the treatment of explosion injury, establish a standard, accurately
Explosion injury cell model have great importance.
Summary of the invention
It is an object of the invention in view of the shortcomings of the prior art, providing a kind of explosion injury cell model established
Experimental provision and experimental method.
The purpose of the present invention can be realized by following technical solutions:
A kind of experimental provision for establishing explosion injury cell model, including hanging ring, lifting rope, suspension hook, hanger, quick-fried source rope, cell culture
Board mount and tissue culture plate.The hanging ring is connected by lifting rope with suspension hook, and the suspension hook is welded on hanger, the quick-fried source
Rope is fixed on hanging ring, and the tissue culture plate pedestal upper end is welded on hanger, and lower end is connected and fixed tissue culture plate.
Further, the hanger is 304 stainless steel materials, including outer ring stand, middle frame and interior ring stand three round branch
Frame, and up and down four be connected and fixed bar, the outer ring stand, middle frame, interior ring stand and be connected and fixed bar in same plane
On, described one end for being connected and fixed bar, centre and the other end are respectively welded in outer ring stand, middle frame and interior ring stand, it is described on
Under be connected and fixed bar on the same line, the left and right is connected and fixed bar on the same line, described to be vertically connected with fixed link institute
Straight line where being connected and fixed bar with the left and right in straight line is mutually perpendicular to.
Preferably, the diameter of the outer ring stand, middle frame and interior ring stand can need to be adjusted according to experiment, and described four
The length that root is connected and fixed bar can also be adjusted according to the diameter of the outer ring stand, middle frame and interior ring stand.
Further, the suspension hook is 304 stainless steel materials, including four up and down, be respectively welded at it is described up and down
Left and right four is connected and fixed the junction of the middle frame of bar and the bracket.
Further, the lifting rope includes four up and down, and upper end is fixed on hanging ring, and lower end is connected to above and below described
On four suspension hooks in left and right, four suspension hook being equidistant to hanging ring up and down.
Further, the quick-fried source rope upper end is fixed on hanging ring, and lower end connects quick-fried source, the straight line where the quick-fried source rope
Perpendicular to the plane where the hanger, the center of location point and the tissue culture plate where the quick-fried source is in same plane
On.
Further, the cell culture board mount is 304 stainless steel materials, including support rod, fixed frame, butterfly screws
And frame is welded and fixed in matching nut, the support rod lower end, the post upper is welded on the outer ring stand of the bracket, centre
On frame and interior ring stand, each 8, adjacent supports bar weld and the angle in the outer ring stand center of circle are in 45 degree on the outer ring stand,
Adjacent supports bar weld and the angle in the middle frame center of circle are in 45 degree on the middle frame, adjacent branch on the interior ring stand frame
Strut weld and the angle in the interior ring stand center of circle are in 45 degree, the weld and the outer ring stand of the outer ring stand upper support bar
The folder of straight line where the weld and the middle frame center of circle of straight line where the center of circle and the closest middle frame upper support bar
Angle is in 15 degree, straight line and the closest inner ring where the weld of the middle frame upper support bar and the middle frame center of circle
The angle of straight line is in 15 degree where the weld of frame upper support bar and the interior ring stand center of circle, the butterfly screws and matching nut
Screw and nut connection is formed on fixed frame.
Further, the tissue culture plate is 96 orifice plates, totally 24, is separately mounted to the cell culture board mount
On, screw and nut is formed by the butterfly screws and matching nut and is connected and fixed, the front of the tissue culture plate is towards in
The quick-fried source of the heart.
Preferably, pressure sensor can be set on the cell culture board mount, measure explosion time tissue culture plate institute
Pressure and time change in position.
A kind of experimental method for establishing explosion injury cell model experimental provision of the present invention, comprising the following steps:
Hanger is suspended in experimental site using lifting rope connection hanging ring and suspension hook, fixed quick-fried source rope by hanging ring by step 1.,
The installation for completing experiment previous experiments device prepares；
Step 2. selects suitable cell line to be studied to cultivate cell in the incubator using corresponding culture medium.It explodes
It first 24 hours, goes in 96 porocyte culture plates and cultivates cell, 15000, every hole cell, the preferably culture hole in middle position.?
The explosion same day replaces the cell culture medium of corresponding culture hole on tissue culture plate, avoids generating bubble.With sterile before explosion
The edge of sterile gas permeability polyester film is fixed on cell culture with sterile adhesive tape by gas sealable polyester film tissue culture plate
On plate；
Tissue culture plate is placed on corresponding cell culture board mount by step 3., fast using butterfly screws and matching nut
Speed is fixed, installs quick-fried source and detonation cable, then detonation carries out blast impulse；
Tissue culture plate is removed at once after step 4. explosion, removes sterile gas permeability polyester film, place in the incubator into
Row is incubated for, and then carries out next step research.
The advantageous effects that the present invention has:
A kind of experimental provision for establishing explosion injury cell model of the invention utilizes simple experimental design, and it is thin to realize explosion injury
The foundation of born of the same parents' model；By the circular support of three different-diameters, by tissue culture plate be fixed on three away from quick-fried source it is different away from
From upper, the cell research under different blast impulse pressure is realized, eight cell culture cultures on each circular support are utilized
Board mount realizes the cell research of identical lower eight samples of blast impulse pressure, and using the experimental provision, primary experiment can be with
Three different blast impulse pressure groups are carried out, the research of every group of eight samples is easy to operate, reproducible；Neighbouring three are not
It is not arranged on the same straight line with the tissue culture plate under diameter circular bracket, avoids the tissue culture plate close apart from quick-fried source to remote
The influence of the tissue culture plate at place, experiment accuracy are high.Compared with prior art, experimental provision production is simple, easy to operate；
The explosion injury cell model of foundation, accuracy is high, and controllability is strong, favorable repeatability.
Detailed description of the invention
Fig. 1 is of the invention a kind of to establish explosion injury cell model experimental provision structural schematic diagram.
Fig. 2 is of the invention a kind of to establish explosion injury cell model experimental provision top view.
Fig. 3 is a kind of tissue culture plate supporting structure signal for establishing explosion injury cell model experimental provision of the invention
Figure.
Description of symbols: 1, hanging ring；2, lifting rope；3, suspension hook；4, hanger；4-1, outer ring stand；4-2, middle frame；It is 4-3, interior
Ring stand；4-4, it is connected and fixed bar；5, quick-fried source rope；6, cell culture board mount；6-1, support rod；6-2, fixed frame；6-3, butterfly
Screw；6-4, matching nut；7, tissue culture plate.
Specific embodiment
The present invention will be further described with example with reference to the accompanying drawing.
In the description of the present invention, it is to be understood that, term " center ", " longitudinal direction ", " transverse direction ", "front", "rear",
The orientation or positional relationship of the instructions such as "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is based on attached drawing institute
The orientation or positional relationship shown, is merely for convenience of description of the present invention and simplification of the description, rather than the dress of indication or suggestion meaning
It sets or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as protecting the present invention
The limitation of range.
As shown in Figure 1-3, a kind of experimental provision for establishing explosion injury cell model, including hanging ring 1, lifting rope 2, suspension hook 3, hang
Frame 4, quick-fried source rope 5, cell culture board mount 6 and tissue culture plate 7.The hanging ring 1 is connected by lifting rope 2 with suspension hook 3, described
Suspension hook 3 is welded on hanger 4, and the quick-fried source rope 5 is fixed on hanging ring 1, and 6 upper end of cell culture board mount is welded on hanger
On 4, lower end is connected and fixed tissue culture plate 7.
The hanger 4 is 304 stainless steel materials, including outer ring stand 4-1, middle frame 4-2 and interior ring stand 4-3 tri- round branch
Frame, and up and down four be connected and fixed bar 4-4, the outer ring stand 4-1, middle frame 4-2, interior ring stand 4-3 and be connected and fixed
Bar 4-4 in the same plane, described one end for being connected and fixed bar 4-4, centre and the other end be respectively welded at outer ring stand 4-1, in
Described to be vertically connected with fixed link 4-4 on the same line on structure of an essay 4-2 and interior ring stand 4-3, the left and right is connected and fixed bar 4-4
On the same line, it is described be vertically connected with fixed link 4-4 where to be connected and fixed the place bar 4-4 straight line mutual straight line and the left and right
Vertically.The diameter of the outer ring stand 4-1, middle frame 4-2 and interior ring stand 4-3 can need to be adjusted according to experiment, and described four
The length that root is connected and fixed bar 4-4 can also be adjusted according to the diameter of the outer ring stand 4-1, middle frame 4-2 and interior ring stand 4-3
Section.
The suspension hook 3 is 304 stainless steel materials, including four up and down, is respectively welded at four up and down described
It is connected and fixed the junction of the middle frame 4-3 of bar 4-4 and the bracket.
The lifting rope 2 includes up and down four, and upper end is fixed on hanging ring 1, and lower end is connected to described up and down four
On a suspension hook 3, four being equidistant to hanging ring 1 of suspension hook 3 up and down.
The quick-fried source 5 upper ends of restricting are fixed on hanging ring 1, and lower end connects quick-fried source, the straight line where the quick-fried source rope 5 perpendicular to
Plane where the hanger 4, the center of location point and the tissue culture plate 7 where the quick-fried source is in the same plane.
The cell culture board mount 6 is 304 stainless steel materials, including support rod 6-1, fixed frame 6-2, butterfly screws 6-
Frame 6-2 is welded and fixed in the lower end 3 and matching nut 6-4, the support rod 6-1, and the upper end the support rod 6-1 is welded on the bracket
On 4 outer ring stand 4-1, middle frame 4-2 and interior ring stand 4-3, each 8, adjacent supports bar 6-1 weld on the outer ring stand 4-1
Angle with the center of circle outer ring stand 4-1 is in 45 degree, adjacent supports bar 6-1 weld and the centre on the middle frame 4-2
The angle in the center of circle frame 4-2 is in 45 degree, adjacent supports bar 6-1 weld and the center of circle the interior ring stand 4-3 on the interior ring stand 4-3
Angle is in 45 degree, the weld of the outer ring stand 4-1 upper support bar 6-1 and the center of circle outer ring stand 4-1 place straight line and most adjacent
The angle of straight line where the weld of the close middle frame 4-2 upper support bar 6-1 and the center of circle the middle frame 4-2 is in 15 degree,
Straight line and the closest inner ring where the weld of the middle frame 4-2 upper support bar 6-1 and the center of circle the middle frame 4-2
The angle of straight line where the weld of frame 4-3 upper support bar 6-1 and the center of circle the interior ring stand 4-3 is in 15 degree, the butterfly screws
6-3 forms screw and nut with matching nut 6-4 on fixed frame 6-2 and connects.
The tissue culture plate 7 is 96 orifice plates, totally 24, is separately mounted on the cell culture board mount 6, passes through institute
It states butterfly screws 6-3 and matching nut 6-4 formation screw and nut to be connected and fixed, the front of the tissue culture plate 7 is towards center
Quick-fried source.Pressure sensor can be set on the cell culture board mount 6, measurement 7 position of explosion time tissue culture plate
Pressure size and time change.
A kind of experimental method of experimental provision for establishing explosion injury cell model of the invention, comprising the following steps:
Step 1. connects hanging ring 1 and suspension hook 3 using lifting rope 2, and hanger 4 is suspended to field experiment by hanging ring 1 by fixed quick-fried source rope 5
In ground, the installation for completing experiment previous experiments device prepares；
Step 2. selects suitable cell line to be studied to cultivate cell in the incubator using corresponding culture medium.It explodes
It first 24 hours, goes in 96 porocyte culture plates 7 and cultivates cell, 15000, every hole cell, the preferably culture hole in middle position.
On the day of explosion, the cell culture medium of corresponding culture hole on tissue culture plate 7 is replaced, avoids generating bubble.Nothing is used before explosion
The edge of sterile gas permeability polyester film is fixed on carefully by bacterium gas permeability sealable polyester film tissue culture plate 7 with sterile adhesive tape
On born of the same parents' culture plate 7；
Tissue culture plate 7 is placed on corresponding cell culture board mount 6 by step 3., utilizes butterfly screws 6-3 and mating spiral shell
Female 6-4 is quickly fixed, and installs quick-fried source and detonation cable, then detonation carries out blast impulse；
Tissue culture plate 7 is removed at once after step 4. explosion, removes sterile gas permeability polyester film, is placed in the incubator
It is incubated for, then carries out next step research.
Experimental provision production of the invention is simple, and primary test can carry out three different blast impulse pressure groups, and every group
The research of eight samples, it is easy to operate；The explosion injury cell model of foundation, accuracy is high, and controllability is strong, favorable repeatability.
It is being discussed in detail for a kind of experimental provision for establishing explosion injury cell model of the present invention and experimental method above, needs
, it is noted that the present invention is not limited to the above specific embodiments.Those skilled in the art are not departing from the principle of the invention
Under the premise of various changes and modifications that technical solution is made, fall within the protection scope that claims of the present invention determines
It is interior.
Claims (8)
1. a kind of experimental provision for establishing explosion injury cell model, it is characterised in that: including hanging ring (1), lifting rope (2), suspension hook
(3), hanger (4), quick-fried source rope (5), cell culture board mount (6) and tissue culture plate (7), the hanging ring (1) pass through lifting rope (2)
It is connected with suspension hook (3), the suspension hook (3) is welded on hanger (4), and the quick-fried source rope (5) is fixed on hanging ring (1), described
Cell culture board mount (6) upper end is welded on hanger (4), and lower end is connected and fixed tissue culture plate (7).
2. the experimental provision according to claim 1 for establishing explosion injury cell model, it is characterised in that: the hanger (4)
For 304 stainless steel materials, including three outer ring stand (4-1), middle frame (4-2) and interior ring stand (4-3) circular supports, and up and down
Left and right four is connected and fixed bar (4-4), the outer ring stand (4-1), middle frame (4-2), interior ring stand (4-3) and is connected and fixed bar
(4-4) in the same plane, described one end for being connected and fixed bar (4-4), centre and the other end are respectively welded at outer ring stand (4-
1) described to be vertically connected with fixed link (4-4) on the same line, on middle frame (4-2) and interior ring stand (4-3), the left and right connects
Connect fixed link (4-4) on the same line, it is described to be vertically connected with straight line where fixed link (4-4) and the left and right is connected and fixed bar
Straight line where (4-4) is mutually perpendicular to.
3. the experimental provision according to claim 1 for establishing explosion injury cell model, it is characterised in that: the suspension hook (3)
For 304 stainless steel materials, including four up and down, be respectively welded at it is described up and down four be connected and fixed bar (4-4) with
The junction of the middle frame (4-3) of the bracket.
4. the experimental provision according to claim 1 for establishing explosion injury cell model, it is characterised in that: the lifting rope (2)
Including four up and down, upper end is fixed on hanging ring (1), and lower end is connected on four suspension hooks (3) up and down, described
Four suspension hook (3) being equidistant to hanging ring (1) up and down.
5. the experimental provision according to claim 1 for establishing explosion injury cell model, it is characterised in that: the quick-fried source rope
(5) upper end is fixed on hanging ring (1), and lower end connects quick-fried source, and the straight line where the quick-fried source rope (5) is perpendicular to the hanger (4)
The plane at place, the center of location point and the tissue culture plate (7) where the quick-fried source is in the same plane.
6. the experimental provision according to claim 1 for establishing explosion injury cell model, it is characterised in that: the cell culture
Board mount (6) is 304 stainless steel materials, including support rod (6-1), fixed frame (6-2), butterfly screws (6-3) and matching nut
(6-4), support rod (6-1) lower end are welded and fixed frame (6-2), and support rod (6-1) upper end is welded on the bracket 4
In outer ring stand (4-1), middle frame (4-2) and interior ring stand (4-3), each 8, adjacent supports bar (6-1) on the outer ring stand (4-1)
Weld and the angle in outer ring stand (4-1) center of circle are in 45 degree, adjacent supports bar (6-1) welding on the middle frame (4-2)
Place is in 45 degree with the angle in the center of circle the middle frame (4-2), on the interior ring stand (4-3) adjacent supports bar (6-1) weld and
The angle in interior ring stand (4-3) center of circle be in 45 degree, the weld of outer ring stand (4-1) upper support bar (6-1) and it is described outside
Weld and the centre of straight line and the closest middle frame (4-2) upper support bar (6-1) where the center of circle ring stand (4-1)
The angle of straight line where the center of circle frame (4-2) is in 15 degree, the weld of middle frame (4-2) upper support bar (6-1) with it is described in
The weld and the inner ring of straight line and the closest interior ring stand (4-3) upper support bar (6-1) where the center of circle a structure of an essay (4-2)
The angle of straight line where the center of circle frame (4-3) is in 15 degree, and the butterfly screws (6-3) and matching nut (6-4) are at fixed frame (6-2)
Upper formation screw and nut connection.
7. the experimental provision according to claim 1 for establishing explosion injury cell model, it is characterised in that: the cell culture
Plate (7) is 96 orifice plates, totally 24, is separately mounted on the cell culture board mount (6), passes through the butterfly screws (6-3)
It forms screw and nut with matching nut (6-4) to be connected and fixed, the front of the tissue culture plate (7) is towards the quick-fried source in center.
8. the experimental method of the experimental provision according to claim 1 for establishing explosion injury cell model, it is characterised in that: packet
Include following steps:
Step 1. is using lifting rope (2) connection hanging ring (1) and suspension hook (3), fixed quick-fried source rope (5), by hanging ring (1) by hanger (4)
It is suspended in experimental site, the installation for completing experiment previous experiments device prepares；
Step 2. selects suitable cell line to be studied to cultivate cell in the incubator using corresponding culture medium；It explodes
It first 24 hours, goes on 96 porocyte culture plates (7) and cultivates cell, 15000, every hole cell, the preferably culture in middle position
Hole；On the day of explosion, the cell culture medium of corresponding culture hole in replacement tissue culture plate (7) avoids generating bubble；Before explosion
With sterile gas permeability sealable polyester film tissue culture plate (7), the edge of sterile gas permeability polyester film is consolidated with sterile adhesive tape
It is scheduled on tissue culture plate (7)；
Tissue culture plate (7) is placed on corresponding cell culture board mount (6) by step 3., using butterfly screws (6-3) and
Matching nut (6-4) is quickly fixed, installs quick-fried source and detonation cable, then detonation carries out blast impulse；
Tissue culture plate (7) is removed at once after step 4. explosion, sterile gas permeability polyester film is removed, is placed on incubator
In be incubated for, then carry out next step research.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113637581A (en) * | 2021-07-21 | 2021-11-12 | 重庆大学 | Instantaneous stretching of cell and high temperature are loading equipment in coordination |
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| RU88022U1 (en) * | 2009-05-20 | 2009-10-27 | Российская Федерация, от имени которой выступает Министерство промышленности и торговли Российской Федерации (Минпромторг России) | CARRIER FOR CULTIVATION OF CELLS |
| CN102650509A (en) * | 2011-02-25 | 2012-08-29 | 柴家科 | Explosion experimental system for blast injury |
| US20130186173A1 (en) * | 2012-01-24 | 2013-07-25 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Serv | Device for simulating explosive blast and imaging biological specimen |
| US20160076967A1 (en) * | 2014-09-15 | 2016-03-17 | The Government Of The United States Of America, As Represented By The Secretary Of The Navy | Modular System for Evaluating Helmet and Suspension Materials for Protection of Neural Cells from Simulated Trauma |
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| RU88022U1 (en) * | 2009-05-20 | 2009-10-27 | Российская Федерация, от имени которой выступает Министерство промышленности и торговли Российской Федерации (Минпромторг России) | CARRIER FOR CULTIVATION OF CELLS |
| CN102650509A (en) * | 2011-02-25 | 2012-08-29 | 柴家科 | Explosion experimental system for blast injury |
| US20130186173A1 (en) * | 2012-01-24 | 2013-07-25 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Serv | Device for simulating explosive blast and imaging biological specimen |
| US20160076967A1 (en) * | 2014-09-15 | 2016-03-17 | The Government Of The United States Of America, As Represented By The Secretary Of The Navy | Modular System for Evaluating Helmet and Suspension Materials for Protection of Neural Cells from Simulated Trauma |
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| CN113637581A (en) * | 2021-07-21 | 2021-11-12 | 重庆大学 | Instantaneous stretching of cell and high temperature are loading equipment in coordination |
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