CN113637581A - Instantaneous stretching of cell and high temperature are loading equipment in coordination - Google Patents

Abstract

The invention provides a cell instantaneous stretching and high-temperature cooperative loading device which comprises a test box and a hot air generating device, wherein the test box comprises a box body and an upper cover capable of covering the box body, at least one stretching cavity which is used for culturing cells and is made of elastic materials is arranged in the box body, the stretching cavity is connected with a stretching driving mechanism which drives the stretching cavity to stretch the cells, and hot air provided by the hot air generating device can be conveyed into the test box. The invention realizes the instantaneous stretching of cells by controlling the change of the stretching cavity by the stretching driving mechanism, and the hot air generated by the hot air generating device heats the cells in the stretching cavity in the test box. The invention can realize the single or synergistic effect composite injury of instantaneous cell stretching and high temperature, simulates the damage of the explosion to the cell, better solves the problem that the explosion is difficult to research on the level of cell molecules, and can research the composite injury mechanism of the explosion on the level of the cell.

Description

Instantaneous stretching of cell and high temperature are loading equipment in coordination

Technical Field

The invention belongs to the technical field of bioengineering, and particularly relates to a device for instantaneous cell stretching and high-temperature collaborative loading.

Background

The explosion events are frequently generated in daily life, production practice and some special environments of people, and bring serious influence on the life health and life safety of people. The damage effect by simulating explosion is an important precondition for evaluating the damage effect of the explosion on tissues/cells and deeply knowing the molecular mechanism of the tissues/cells in experimental research.

Explosive damage is usually a compound damage caused by many factors such as temperature, fragmentation, shock wave, etc. In case of explosion, the human body may suffer different injuries, such as burns, lacerations, etc. Contusion and hemorrhage of each organ occur, and morphological changes such as lysosome hyperplasia, mitochondrial swelling and malformation, endoplasmic reticulum expansion and the like occur in cells. Due to the lack of destructive effect devices simulating explosion at the tissue/cell level, the current research on explosion injury is mostly focused on the animal and dummy level, the research on explosion injury at the cell/tissue level is less, and the specific molecular mechanism is less clear.

Disclosure of Invention

The invention aims to solve the technical problems in the prior art, and aims to provide an in-vitro cell instantaneous stretching and high-temperature cooperative loading device.

In order to achieve the purpose, the invention adopts the following technical scheme: the utility model provides a cell instantaneous stretching and high temperature load equipment in coordination, includes proof box and hot-blast generating device, and the proof box includes the box and can the lid closes the upper cover on the box, is equipped with at least one tensile chamber that is used for cultivateing the cell and is made by elastic material in the box, and tensile chamber is connected with the tensile actuating mechanism of its extension of drive with tensile cell, and the hot-blast that hot-blast generating device provided can be carried to proof box inside.

The invention realizes the instantaneous stretching of cells by controlling the change of the stretching cavity by the stretching driving mechanism, and the hot air generated by the hot air generating device heats the cells in the stretching cavity in the test box. The invention can realize the single or synergistic effect composite injury of instantaneous cell stretching and high temperature, simulates the damage of the explosion to the cell, better solves the problem that the explosion is difficult to research on the level of cell molecules, and can research the composite injury mechanism of the explosion on the level of the cell.

In a preferred embodiment of the invention, each drawing chamber is driven by a respective set of drawing drive mechanisms, or all drawing chambers are driven by the same set of drive mechanisms. According to the actual situation, one or more sets of stretching driving mechanisms are arranged to drive the stretching cavity to move.

In a preferred embodiment of the invention, a first mounting block and a second mounting block are arranged in the test box, one end of the stretching cavity is fixedly connected with the first mounting block, the other end of the stretching cavity is fixedly connected with the second mounting block, the first mounting block is fixedly connected with the box body, the second mounting block is connected with a stretching driving mechanism, and the stretching driving mechanism drives the second mounting block to move to be close to or far away from the first mounting block.

Among the above-mentioned technical scheme, the both ends in all tensile chambeies are fixed respectively on first installation piece and second installation piece, and tensile actuating mechanism drives the second installation piece rectilinear motion alright realize that the cell is tensile from this, easy operation.

In a preferred embodiment of the invention, the stretching driving mechanism comprises a linear motor, an output shaft of the linear motor is connected with the second mounting block, and the linear motor is arranged outside the test box. The linear motor is arranged outside the test box, and does not occupy the internal space of the test box; and linear electric motor self temperature also can rise when the during operation, establishes and to reduce the influence to the inside temperature of proof box outside the proof box, makes the inside temperature of proof box more stable.

In a preferred embodiment of the present invention, the hot air generator is disposed outside the test chamber, and the test chamber is provided with a hot air window through which hot air supplied from the hot air generator can be delivered into the test chamber.

In the technical scheme, the hot air generating device is arranged outside the test box, so that the internal space of the test box is not occupied; and hot-blast generating device self temperature also can rise when the work, establishes to reduce the influence to the inside temperature of proof box outside the proof box, makes the inside temperature of proof box more stable.

In another preferred embodiment of the present invention, the hot wind generating means includes a heating module and a fan.

In another preferred embodiment of the invention, a temperature sensor is arranged in the test box, and a signal output end of the temperature sensor is connected with an output control end of the hot air generating device.

Among the above-mentioned technical scheme, through setting up the inside temperature variation of temperature sensor detection test case, the hot-blast generating device of being convenient for adjusts, makes the inside temperature of test case keep at the settlement temperature, makes the test result more accurate.

In another preferred embodiment of the invention, the hot air generating device further comprises a controller and a human-computer interaction display connected with the controller, wherein the signal output end of the temperature sensor is connected with the temperature input end of the controller, the stretching control end of the controller is connected with the enabling end of the stretching driving mechanism, and the temperature control end of the controller is connected with the enabling end of the hot air generating device.

Among the above-mentioned technical scheme, make this equipment degree of automation higher through controller and human-computer interaction display, the operator only need in the operating mode of the key operation alright control this equipment of human-computer interaction display, more simple and convenient.

In another preferred embodiment of the present invention, the upper cover has a viewing window made of a transparent material. Through setting up the observation window, the inside condition of proof box is known in real time to the people of being convenient for.

In another preferred embodiment of the invention, the test chamber is provided with a carbon dioxide vent, and carbon dioxide gas provided by external equipment can enter the interior of the test chamber through the carbon dioxide vent.

Among the above-mentioned technical scheme, external equipment provides the carbon dioxide that the concentration is fit for cell growth for the proof box is inside, does benefit to the cell growth reproduction in the tensile chamber, makes the test result more accurate.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

FIG. 1 is a front perspective view of a device for simultaneous stretching and high temperature loading of cells according to an embodiment of the present disclosure, wherein the upper cover is in an open state.

FIG. 2 is a schematic rear perspective view of a device for simultaneous cell stretching and high temperature loading, according to an embodiment of the present disclosure, with the upper lid closed.

Fig. 3 is an enlarged schematic view of the tensile chamber of fig. 1 mounted in a test chamber.

Reference numerals in the drawings of the specification include: the device comprises a test box 10, a box body 11, a first mounting block 111, a second mounting block 112, a fixing column 113, an upper cover 12, an observation window 121, a handle 122, a hot air window 13, a carbon dioxide vent 14, a stretching cavity 20, a stretching driving mechanism/linear motor 30, a motor mounting seat 31, a hot air generating device 40, a fan 41, a heating module 42, a temperature sensor 50 and a support box 60.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "vertical", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the present invention.

In the description of the present invention, unless otherwise specified and limited, it is to be noted that the terms "mounted," "connected," and "connected" are to be interpreted broadly, and may be, for example, a mechanical connection or an electrical connection, a communication between two elements, a direct connection, or an indirect connection via an intermediate medium, and specific meanings of the terms may be understood by those skilled in the art according to specific situations.

The invention provides a device for the instantaneous stretching and high-temperature synergic loading of cells, which is shown in figures 1 and 2, and in a preferred embodiment of the invention, the device comprises a test box 10 and a hot air generating device 40, wherein the test box 10 comprises a box body 11 and an upper cover 12 capable of covering the box body 11. At least one stretching cavity 20 made of an elastic material for culturing cells is arranged in the box body 11, seven stretching cavities 20 are arranged in fig. 1, the seven stretching cavities 20 are arranged at intervals along the length direction of the test chamber 10, the stretching cavities 20 can be made of an elastic material such as silicon gel, rubber and the like, for example, the stretching cavities 20 are made of transparent silicon gel. The stretching chamber 20 is connected to a stretching driving mechanism 30 for driving the stretching chamber to stretch the cell, and hot air supplied from a hot air generating device 40 can be supplied into the test chamber 10.

When using the apparatus, first, seven stretching chambers 20 are installed in the housing 11, cells are cultured in the stretching chambers 20, and the upper cover 12 is closed. Next, the stretching frequency of the stretching driving mechanism 30 and the temperature of the hot air supplied from the hot air generating device 40 are set, and then the stretching driving mechanism 30 and the hot air generating device 40 are activated to achieve stretching of the stretching chamber 20 and temperature variation within the test chamber 10. Cells grow at the bottom of the stretching cavity 20 in an adherent manner, and when the stretching cavity 20 stretches, the cells stretch along with the movement of the stretching cavity 20 so as to simulate the laceration of the cells during explosion; the hot air generated by the hot air generator 40 heats the cells in the stretching cavity 20 in the test chamber 10, and the burning of the cells by high temperature during explosion is simulated.

The tensile strain range of the device is 0-50%, the time required for reaching the maximum strain is not more than 0.3s, the instantaneous stretching of cells is realized, and the mechanism research of cell injury in a very short time can be carried out. The device can realize single or compound injury of cells under instantaneous stretching and high temperature, and can research the compound injury mechanism of explosion at the cellular level. In addition, the number of the stretching cavities 20 is multiple, the damage condition of cells in the stretching cavities 20 can be observed simultaneously in one test, and the efficiency is high.

As shown in fig. 1-3, in this embodiment, all of the drawing chambers 20 are driven by the same set of drive mechanisms. Specifically, the test chamber 10 is provided with a first mounting block 111 and a second mounting block 112 located behind the first mounting block 111, the first mounting block 111 is fixedly connected to the box body 11, and the second mounting block 112 is connected to the tensile driving mechanism 30. A plurality of fixing columns 113 are fixed on the first mounting block 111 and the second mounting block 112, four corners of the stretching cavity 20 are respectively provided with a through hole matched with the fixing column 113, two corners of the front ends of the seven stretching cavities 20 are fixedly connected with the first mounting block 111 through the fixing columns 113, and two corners of the rear ends of the seven stretching cavities 20 are also fixedly connected with the second mounting block 112 through the fixing columns 113. The tensile driving mechanism 30 is provided at the rear side of the outside of the test chamber 10, and the second mounting block 112 is driven to move linearly to approach or separate from the first mounting block 111 by the tensile driving mechanism 30. When the tension driving mechanism 30 drives the second mounting block 112 to move backward away from the first mounting block 111, the tension chamber 20 is elongated to achieve cell tension.

As shown in fig. 2, in the present embodiment, the drawing drive mechanism 30 is preferably a linear motor, the linear motor 30 is preferably a servo motor, and an output shaft of the linear motor 30 is preferably fixed to the second mounting block 112. The rear side of the test box 10 is fixedly connected with a supporting box 60 with an opening at the rear end, the opening at the rear end of the supporting box 60 is fixedly connected with a motor mounting seat 31, and the linear motor 30 is mounted at the rear side of the motor mounting seat 31 through bolts.

It should be noted that each stretching chamber 20 may be driven by one set of stretching driving mechanism 30, that is, seven sets of stretching driving mechanisms 30 are provided, and the stretching operation of each stretching chamber 20 may be performed independently. The stretching driving mechanism 30 may be a mechanism capable of driving the second mounting block 112 to move linearly, such as a hydraulic cylinder or an air cylinder, in addition to the linear motor described above.

In another preferred embodiment of the present invention, as shown in fig. 1 and 2, the upper cover 12 has a viewing window 121 made of a transparent material, for example, the viewing window 121 is made of transparent glass or transparent plastic. Thereby facilitating real-time observation of the test conditions in test chamber 10.

In another preferred embodiment of the present invention, as shown in fig. 1 and 2, the test chamber 10 is provided with carbon dioxide vents 14, for example, one carbon dioxide vent 14 is provided on each of the left and right sides of the upper cover 12, carbon dioxide gas supplied from an external device can enter the interior of the test chamber 10 through the carbon dioxide vents 14, and the carbon dioxide gas is supplied at a concentration suitable for cell growth.

In another preferred embodiment of the present invention, as shown in fig. 1 and 2, the hot air generating device 40 is provided outside the test chamber 10, for example, the hot air generating device 40 is installed at the outside of the top of the support box 60. The box body 11 of the test chamber 10 is provided with a hot air window 13, and hot air provided by the hot air generating device 40 can be conveyed into the test chamber 10 through the hot air window 13.

In the present embodiment, the hot air generator 40 includes a heating module 42 and a fan 41, the heating module 42 is provided adjacent to the hot air window 13, and the fan 41 is provided on the rear side of the heating module 42.

In another preferred embodiment of the present invention, as shown in fig. 1, a temperature sensor 50 is provided in the test chamber 10, and a signal output terminal of the temperature sensor 50 is connected to an output control terminal of the heating module 42 of the hot air generating device 40. The temperature change inside the test chamber 10 is monitored by the temperature sensor 50, and the heating module 42 is controlled by this temperature feedback information so that the temperature inside the test chamber 10 is maintained at a set value.

In another preferred embodiment of the present invention, the device further comprises a controller and a human-machine-interaction display (not shown) connected to the controller. The signal output end of the temperature sensor 50 is connected with the temperature input end of the controller, the stretching control end of the controller is connected with the enabling end of the linear motor 30, and the temperature control end of the controller is connected with the enabling end of the heating module 42 of the hot air generating device 40.

The key operation of the man-machine interaction display sends an instruction to the controller to control the whole equipment, and the controller receives corresponding functional commands and data to control the linear motor 30 and the heating module 42. The running speed of the linear motor 30 is calculated according to the stretching distance and the pulling frequency, the stretching position distance can be detected by adopting a photoelectric detection switch, and the reliability and the accuracy of the position are ensured.

In the description herein, reference to the description of the terms "preferred embodiment," "one embodiment," "some embodiments," "an example," "a specific example" or "some examples" or the like means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

While embodiments of the invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims (10)

1. The device is characterized by comprising a test box and a hot air generating device, wherein the test box comprises a box body and an upper cover capable of covering the box body, at least one stretching cavity which is used for culturing cells and is made of elastic materials is arranged in the box body, the stretching cavity is connected with a stretching driving mechanism which drives the stretching cavity to stretch the cells, and hot air provided by the hot air generating device can be conveyed to the interior of the test box.

2. The device for instantaneous stretching and high-temperature cooperative loading of cells according to claim 1, wherein each stretching cavity is driven by a set of stretching driving mechanism, or all stretching cavities are driven by the same set of driving mechanism.

3. The instantaneous stretching and high-temperature collaborative loading equipment for the cells according to claim 1, wherein a first mounting block and a second mounting block are arranged in the test box, one end of the stretching cavity is fixedly connected with the first mounting block, the other end of the stretching cavity is fixedly connected with the second mounting block, the first mounting block is fixedly connected with the box body, the second mounting block is connected with the stretching driving mechanism, and the stretching driving mechanism drives the second mounting block to move to be close to or far away from the first mounting block.

4. The device for instantaneous stretching and high-temperature cooperative loading of cells according to claim 3, wherein the stretching driving mechanism comprises a linear motor, an output shaft of the linear motor is connected with the second mounting block, and the linear motor is arranged outside the test chamber.

5. The apparatus for instantaneous cell stretching and high-temperature cooperative cell loading according to claim 1, wherein the hot air generator is disposed outside the test chamber, and a hot air window is disposed on the test chamber, and hot air provided by the hot air generator can be delivered to the inside of the test chamber through the hot air window.

6. The device for instantaneous stretching and high-temperature collaborative loading of cells according to claim 1, wherein the hot air generating device comprises a heating module and a fan.

7. The device for the instantaneous stretching and high-temperature cooperative loading of cells according to any one of claims 1 to 6, wherein a temperature sensor is arranged in the test box, and a signal output end of the temperature sensor is connected with an output control end of the hot air generating device.

8. The device for instantaneous stretching and high-temperature cooperative loading of cells according to claim 7, further comprising a controller and a human-computer interaction display connected with the controller, wherein the signal output end of the temperature sensor is connected with the temperature input end of the controller, the stretching control end of the controller is connected with the enabling end of the stretching driving mechanism, and the temperature control end of the controller is connected with the enabling end of the hot air generating device.

9. The device for the instantaneous stretching and high-temperature cooperative loading of cells according to any one of claims 1 to 6, wherein the upper cover is provided with a viewing window made of a transparent material.

10. The device for the instantaneous stretching and high-temperature co-loading of cells according to any one of claims 1 to 6, wherein a carbon dioxide vent is arranged on the test chamber, and carbon dioxide gas provided by an external device can enter the interior of the test chamber through the carbon dioxide vent.

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