CN110055179B

CN110055179B - Carbon dioxide incubator for bioengineering

Info

Publication number: CN110055179B
Application number: CN201910490187.0A
Authority: CN
Inventors: 王利军
Original assignee: Hangzhou Jianan Biotechnology Co ltd
Current assignee: Hangzhou Jianan Biotechnology Co ltd
Priority date: 2019-06-06
Filing date: 2019-06-06
Publication date: 2022-07-05
Anticipated expiration: 2039-06-06
Also published as: CN110055179A

Abstract

The invention discloses a carbon dioxide incubator for bioengineering, which comprises a first box body and a first sealing door arranged on the side wall of the first box body, wherein an equipment box is arranged on one side of the first box body, a display screen and an operation panel are arranged on the equipment box, a second box body is arranged in the first box body, a plurality of first sliding grooves are formed in the inner wall of the second box body, a bearing plate is arranged in each first sliding groove, a second sliding groove is formed in the inner wall of the first box body, a first sliding block matched with the second sliding groove is arranged on the side wall of the second box body, a driving piece used for pushing the first sliding block to move upwards is arranged at the bottom of each second sliding groove, and a sealing piece is arranged on the side wall of the first sliding block; through the up-and-down motion of second box for first box, carry out appropriate adjustment to the volume of incubator, satisfy the requirement of cultivateing different quantity cells, promote the application scope of incubator.

Description

Carbon dioxide incubator for bioengineering

Technical Field

The invention belongs to the technical field of bioengineering, and particularly relates to a carbon dioxide incubator for bioengineering.

Background

The carbon dioxide incubator is used for culturing cells in vitro by simulating and forming a growth environment similar to cells/tissues in organisms in the incubator body. The incubator requires stable temperature (37 ℃), stable CO2 level (5%), constant pH value (pH value: 7.2-7.4) and higher relative saturation humidity (95%), and the quality of the culture result in the incubator directly influences the success or failure of the subsequent tests. The volume of the existing incubator is constant, but due to different requirements of cultivation, the number of cultivation at each time can be changed according to actual conditions, so that the problems that most of space in the incubator is not utilized and the space in the incubator is not sufficiently utilized exist, and the practicability of the incubator is influenced.

Disclosure of Invention

The invention provides a carbon dioxide incubator for bioengineering, aiming at overcoming the defects of the prior art.

In order to achieve the purpose, the invention adopts the following technical scheme: a carbon dioxide incubator for bioengineering comprises a first box body and a first sealing door arranged on the side wall of the first box body, wherein an equipment box is arranged on one side of the first box body, a display screen and an operation panel are arranged on the equipment box, a second box body is arranged in the first box body, a plurality of first sliding grooves are formed in the inner wall of the second box body, a bearing plate is arranged in each first sliding groove, a second sliding groove is formed in the inner wall of the first box body, a first sliding block matched with the second sliding groove is arranged on the side wall of the second box body, a driving piece used for pushing the first sliding block to move upwards is arranged at the bottom of each second sliding groove, and a sealing piece is arranged on the side wall of each first sliding block; cells or tissues are placed on the bearing plate for culturing, when fewer cells are cultured, the first sliding block is pushed to the bottom of the second sliding groove, the second box body moves to the bottom of the first box body, the side walls of the first box body and the second box body are mutually overlapped, the height of the incubator is reduced, and therefore the volume of the incubator is reduced, the interior of the incubator is easier to regulate and control after the volume of the incubator is reduced, and the balance of the concentration, the temperature and the humidity of carbon dioxide in the incubator is convenient to maintain; when more cells are cultured, the driving piece drives the second box body to move upwards, so that the first sliding block moves to the top of the second sliding groove, the side walls of the first box body and the second box body are not overlapped any more, the height of the incubator is increased, the space inside the incubator is increased, more spaces are formed in the incubator for placing the bearing plates, and more cells can be cultured in the incubator conveniently; the volume of the incubator is properly adjusted through the up-and-down movement of the second box body relative to the first box body, the requirement of culturing cells with different numbers is met, and the application range of the incubator is widened; the sealing member is done sealed the processing to the contact point of first slider with the second spout, mutually supports with first sealing door and forms good sealed environment in the incubator, avoids external air to enter into the incubator and causes the influence to the steady state in the incubator, makes each item index in the incubator accord with the cultivation standard, increases the cell culture survival rate, promotes the cultivation effect in the incubator.

A guide plate is arranged at the bottom of the first box body, a first movable cavity matched with the guide plate is arranged on the side wall of the second box body, and a plurality of third sliding grooves matched with the bearing plate are formed in the inner wall of the guide plate; the guide plate is matched with the first movable cavity to provide a guide effect for the second box body, so that the second box body can perform lifting motion more stably, and the moving stability of the second box body is improved; after the second box body moves to the top of the first box body, the bearing plates are arranged in the third sliding grooves, so that the number of the bearing plates in the incubator is increased, more cells can be cultured in the incubator conveniently, and the application range of the incubator is widened; under the cooperation of baffle and first activity chamber, increase the sealed effect between first box and the second box, avoid the air to enter into the incubator and cause the influence to the steady state in the incubator, guarantee the cultivation effect in the incubator.

A second movable cavity is arranged in the first sliding block, a limiting block is arranged in the second movable cavity, a fourth sliding groove is formed in the inner wall of the second movable cavity, a second sliding block matched with the fourth sliding groove is arranged on the side wall of the limiting block, a limiting spring is arranged on the side wall of the second sliding block, and a rubber pad is arranged at one end of the limiting block; when the position of the second box body is determined and does not move any more, the limiting block extends out of the second movable cavity, a rubber pad at one end of the limiting block is in contact with the inner wall of the second sliding groove, the contact area of the limiting block and the second sliding groove is increased under the action of the rubber pad, the sealing effect of the first sliding block and the second sliding groove is improved, meanwhile, under the action of the rubber pad, the friction force between the first sliding block and the inner wall of the second sliding groove is increased, the burden of a driving piece is reduced, the second box body is placed on the top of the first box body more stably, and the use reliability of the incubator is guaranteed; under spacing spring's effect, make the stopper produce the trend toward the second activity chamber outside motion to make the stopper produce the extrusion effect to second spout inner wall, increase the contact effect of rubber pad and second spout inner wall, the sealed effect of the first slider of further promotion and second spout junction.

A third movable cavity is arranged on the side wall of the second box body and is communicated with the second movable cavity, a through hole is formed in the side wall of the third movable cavity, a rotating shaft is arranged in the through hole in a penetrating mode, the rotating shaft is rotatably connected to the inner wall of the third movable cavity, an annular groove is formed in the rotating shaft, a connecting rope is wound on the annular groove, and one end of the connecting rope is arranged on the limiting block; when the volume of the incubator is adjusted, the rotating shaft is rotated, the connecting rope is wound on the rotating shaft, the limiting block is pulled to move towards the second movable cavity after the length of the connecting rope is reduced, the limiting block is separated from the inner wall of the second chute, the driving piece is enabled to easily push the first sliding block to move in the second chute, the volume of the incubator is adjusted, and the incubator is enabled to adapt to the culture requirements of different material conveying; the pivot is released to the second box after moving to suitable position, and spacing spring promotes the stopper toward the outward movement, makes the stopper pulling connect the rope and removes, and the pivot is connecting automatic the rotating under the rope drives, makes stopper and the automatic formation cooperation of second spout inner wall, plays the fixed action with the driving piece cooperation to the second box, promotes the sealed cooperation effect of second box and first box.

A rotating disc is arranged at one end of the rotating shaft, a notch is formed in the rotating disc, a fifth sliding groove is formed in the side wall of the second box body, a third sliding block is arranged in the fifth sliding groove, and a limiting plate matched with the notch is arranged on the third sliding block; the rotating shaft is easier to rotate under the arrangement of the rotating disc, so that the limiting block is convenient to control; when the position of the second box body is adjusted, the rotary disc is rotated, the connecting rope is wound on the rotating shaft to drive the limiting block to move towards the second movable cavity, then the limiting plate is inserted into the notch, and the rotary disc is fixed, so that the position of the second box body is adjusted under the action of the driving piece; after the position of second box is confirmed, take out the limiting plate from the breach, drive pivot autogiration under limiting spring's effect, make the limiting block contact with second spout inner wall, insert the limiting plate in the breach afterwards, play the fixed action to the carousel under the limiting plate effect, prevent that the carousel from rotating to drive the stopper and remove, guarantee the cooperation effect of limiting block and second spout.

A through cavity is formed in the first sliding block, an air inlet nozzle is arranged at the top of the through cavity, first grooves are formed in two ends of the through cavity respectively, the sealing element is an air bag arranged in the first grooves, and the air bag is communicated with the through cavity; after the position of second box is confirmed, fill into the air from the suction nozzle department toward leading to the intracavity, make the air enter into the gasbag in, make the gasbag swell under the effect of atmospheric pressure and produce deformation, contact with second spout inner wall behind the gasbag volume grow, stop up the gap department of first slider and second spout, avoid external air to enter into the incubator in, mutually support with the stopper and play the fixed action to first slider, guarantee the cooperation effect of first box and second box.

The bottom of the first box body is provided with a cavity, a bearing cavity assembly is arranged in the cavity and comprises a top plate, a bottom plate and a sealing film, the top plate and the bottom plate are connected through a plurality of first connecting rods, each two first connecting rods are arranged in a crossed mode to form a group of supporting assemblies, one end of each first connecting rod is hinged to the top plate, and the other end of each first connecting rod is hinged to the bottom plate; when the bearing plate needs to be taken out of the incubator, the bearing cavity assembly is pulled out of the cavity, the bearing cavity is arranged on one side of the first sealing door, the top plate is pulled upwards, the bottom plate is separated from the top plate, the first connecting rod provides supporting force for the top plate, the cavity is formed between the bottom plate and the top plate, carbon dioxide is filled between the bottom plate and the top plate, environmental parameters in the bearing cavity are adjusted, various indexes in the bearing cavity are the same as those in the incubator, then the bearing plate is pushed into the bearing cavity, excessive action is taken on the bearing plate through the bearing cavity, and the influence of the incubator on the culture environment in the incubator due to the fact that the incubator is directly opened to enable air to enter the incubator is avoided; because the space of the adapting cavity is small, the regulation and control of the environment in the adapting cavity are easy to regulate and control the environment in the culture cavity again, so that the culture cavity is in a relatively stable environment, and the survival rate of cell culture is improved; the bearing cavity assembly is stored through the cavity, so that the bearing cavity assembly is placed in the cavity when not in use, the space occupied by the culture cavity is reduced, and the use of the culture box is more convenient.

The sealing film is made of an elastic material, a plurality of supporting springs are arranged on the bottom plate, a connecting film is arranged on the side wall of the bottom plate, the connecting film is made of an elastic material, and the other end of the connecting film is fixedly connected to the top plate; the supporting spring has a supporting function on the top plate, after the bearing cavity assembly is taken out of the cavity, the top plate is pushed to move upwards under the action of the supporting spring, so that the top plate and the bottom plate are separated, the connecting film has a sealing function on the side wall of the bearing cavity, the bearing cavity forms a sealed space, and the environment in the bearing cavity is conveniently controlled; through elastic material's setting, make seal membrane and connection membrane receive tensile when the roof removes, make connecting plate and seal membrane be connected with roof and bottom plate all the time, play good sealed effect to accepting the chamber.

A fourth movable cavity is arranged in the first sealing door, a second sealing door is arranged in the fourth movable cavity, a plurality of first through grooves matched with the bearing plate are formed in the first sealing door, a second through groove matched with the first through grooves is formed in the second sealing door, a first baffle is arranged in the first through groove, and a second baffle is arranged in the second through groove; when the second sealing door is arranged in the fourth movable cavity, the first through groove and the second through groove are aligned, when one bearing plate in the culture cavity needs to be taken, the bearing cavity assembly is taken out of the cavity, the bearing cavity assembly moves to one side of the corresponding bearing plate position, the environment in the bearing cavity is adjusted, the environment in the bearing cavity is the same as the environment in the culture cavity, the first baffle and the second baffle corresponding to one side of the bearing plate are opened, the bearing plate is pushed out from the first through groove and the second through groove, the bearing plate is arranged in the bearing cavity, when the bearing plate completely enters the bearing cavity, the first baffle and the second baffle are closed, the sealing membrane is opened, the bearing plate is taken out of the bearing cavity, cells on the bearing plate are picked up, and the influence of air directly entering the culture box to the environment in the culture box when one bearing plate is taken is avoided, ensure the steady state environment in the incubator and improve the survival rate of cells.

A fourth sliding block is arranged on the side wall of the top plate, a fifth sliding block is arranged on the side wall of the bottom plate, the fourth sliding block and the fifth sliding block have the same structure, a sixth sliding groove matched with the fourth sliding block is arranged on the first sealing door, and a seventh sliding groove matched with the fourth sliding block is arranged on the second sealing door; under the mutually supporting of slider and spout, the messenger accepts the chamber subassembly and can remove along the sealing door surface to make the sealing chamber subassembly move corresponding position department on the sealing door as required, be convenient for take out everywhere in the incubator accept the board, promote the convenience that the incubator used.

The invention has the following advantages: through the up-and-down motion of second box for first box, carry out appropriate adjustment to the volume of incubator, satisfy the requirement of cultivateing different quantity cells, promote the application scope of incubator.

Drawings

FIG. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a first cross-sectional view of the present invention.

Fig. 3 is an enlarged view of a point a in fig. 2.

Fig. 4 is an enlarged view of fig. 2 at B.

FIG. 5 is a second cross-sectional view of the present invention.

Fig. 6 is an enlarged view of fig. 5 at C.

Fig. 7 is a third schematic cross-sectional view of the present invention.

Fig. 8 is an enlarged view of fig. 7 at D.

Fig. 9 is a fourth schematic cross-sectional view of the present invention.

Fig. 10 is an enlarged view of fig. 9 at E.

Fig. 11 is a cross-sectional view of the fifth embodiment of the present invention.

Fig. 12 is an enlarged view of fig. 11 at F.

Detailed Description

As shown in fig. 1-12, a carbon dioxide incubator for biological engineering comprises a first box body 1 and a first sealing door 3 arranged on the side wall of the first box body 1, wherein an equipment box 4 is arranged on one side of the first box body 1, a display screen and an operation panel are arranged on the equipment box 4, various parameters in the incubator are displayed on the display panel, a plurality of buttons are arranged on the operation panel and used for controlling various motions in the incubator, the basic structure of the carbon dioxide incubator is the same as that of the carbon dioxide incubator in the prior art, and the carbon dioxide incubator comprises various sensors and an air supply system, which are not described again; a second box body 2 is arranged in the first box body 1, a plurality of first sliding grooves are formed in the inner wall of the second box body 2, a bearing plate 21 is arranged in each first sliding groove, second sliding grooves 11 are respectively formed in the inner walls of two sides of the first box body 1, a first sliding block 22 matched with the second sliding grooves 11 is arranged on the side wall of the second box body 2, a driving piece used for pushing the first sliding block 22 to move upwards is arranged at the bottom of each second sliding groove 11, and a sealing piece is arranged on the side wall of each first sliding block 22; cells or tissues are placed on the bearing plate for culturing, when fewer cells are cultured, the first sliding block is pushed to the bottom of the second sliding groove, the second box body moves to the bottom of the first box body, the side walls of the first box body and the second box body are mutually overlapped, the height of the incubator is reduced, and therefore the volume of the incubator is reduced, the interior of the incubator is easier to regulate and control after the volume of the incubator is reduced, and the balance of the concentration, the temperature and the humidity of carbon dioxide in the incubator is convenient to maintain; when more cells are cultured, the driving part drives the second box body to move upwards, so that the first sliding block moves to the top of the second sliding groove, the side walls of the first box body and the second box body are not overlapped any more, the height of the incubator is increased, the space in the incubator is increased, more spaces are formed in the incubator for placing the bearing plates, and more cells can be cultured in the incubator conveniently; the volume of the incubator is properly adjusted through the up-and-down movement of the second box body relative to the first box body, the requirement of culturing cells with different numbers is met, and the application range of the incubator is widened; the sealing member is done sealed the processing to the contact point of first slider with the second spout, mutually supports with first sealing door and forms good sealed environment in the incubator, avoids external air to enter into the incubator and causes the influence to the steady state in the incubator, makes each item index in the incubator accord with the cultivation standard, increases the cell culture survival rate, promotes the cultivation effect in the incubator.

The driving piece is an air pressure rod 12 arranged at the bottom of the second sliding chute, a third through groove is formed in the side wall of the second sliding chute, a second connecting rod 13 penetrates through the third through groove, a push rod is arranged on the second connecting rod, and the second connecting rod is connected with a switch of the air pressure rod; under the action of the second connecting rod, switches of the air pressure rods in the second sliding grooves on the left side and the right side are connected to the push rod, so that the push rod operates the two air pressure rods simultaneously when being pressed down; when the second box body needs to be lifted, the push rod is pressed downwards, and the switches of the two air pressure rods push the first sliding block to move upwards after being opened, so that the second box body is lifted upwards, and the space of the incubator is increased; when the second box is pressed into the first box body, the push rod is pressed down, the second box body is pressed down, the first sliding block moves downwards along the second sliding groove, the second box body is pressed into the first box body, the space of the incubator is reduced, the waste of resources caused by the fact that the space of the incubator is too large is avoided, and the environment of the incubator is regulated and controlled more conveniently.

A guide plate 14 is arranged at the bottom of the first box body 1, a first movable cavity matched with the guide plate 14 is arranged on the side wall of the second box body 2, and a plurality of third sliding grooves matched with the bearing plate 21 are formed in the inner wall of the guide plate 14; the guide plate is matched with the first movable cavity to provide a guide effect for the second box body, so that the second box body can perform lifting motion more stably, and the moving stability of the second box body is improved; after the second box body moves to the top of the first box body, the bearing plates are arranged in the third sliding grooves, so that the number of the bearing plates in the incubator is increased, more cells can be cultured in the incubator conveniently, and the application range of the incubator is widened; under the cooperation of baffle and first activity chamber, increase the sealed effect between first box and the second box, avoid the air to enter into the incubator and cause the influence to the steady state in the incubator, guarantee the cultivation effect in the incubator.

The second chute and the third chute are internally provided with driving wheels, the first box body and the second box body are internally provided with driving motors which are matched with the driving wheels in a driving way, when the bearing plate is arranged in the second chute or the third chute, the driving wheels are contacted with the side wall of the bearing plate, and when the driving motors drive the driving wheels to rotate, the bearing plate can be pushed out from the second chute or the third chute.

A second movable cavity is arranged in the first sliding block 22, a limiting block 26 is arranged in the second movable cavity, the second movable cavity penetrates through the first sliding block, two groups of limiting blocks are arranged at two ends of the second movable cavity respectively, a fourth sliding groove 221 is arranged on the inner wall of the second movable cavity, a second sliding block 261 matched with the fourth sliding groove 221 is arranged on the side wall of the limiting block 26, a limiting spring 262 is arranged on the side wall of the second sliding block 261, and a rubber pad is arranged at one end of the limiting block 26; when the position of the second box body is determined and does not move any more, the limiting block extends out of the second movable cavity, a rubber pad at one end of the limiting block is in contact with the inner wall of the second sliding groove, the contact area of the limiting block and the second sliding groove is increased under the action of the rubber pad, the sealing effect of the first sliding block and the second sliding groove is improved, meanwhile, under the action of the rubber pad, the friction force between the first sliding block and the inner wall of the second sliding groove is increased, the burden of a driving piece is reduced, the second box body is placed on the top of the first box body more stably, and the use reliability of the incubator is guaranteed; under limiting spring's effect, make the stopper produce the trend toward the second activity chamber outside motion to make the stopper produce the extrusion effect to second spout inner wall, increase the contact effect of rubber pad and second spout inner wall, the sealed effect of the first slider of further promotion and second spout junction.

A third movable cavity 23 is arranged on the side wall of the second box body 2, the third movable cavity 23 is communicated with the second movable cavity, a through hole is arranged on the side wall of the third movable cavity 23, a rotating shaft 24 is arranged in the through hole in a penetrating manner, the rotating shaft 24 is rotatably connected to the inner wall of the third movable cavity 23, an annular groove 241 is arranged on the rotating shaft 24, a connecting rope is wound on the annular groove 241, and one end of the connecting rope is arranged on the limiting block 26; when the volume of the incubator is adjusted, the rotating shaft is rotated, the connecting rope is wound on the rotating shaft, the limiting block is pulled to move towards the second movable cavity after the length of the connecting rope is reduced, the limiting block is separated from the inner wall of the second chute, the driving piece is enabled to easily push the first sliding block to move in the second chute, the volume of the incubator is adjusted, and the incubator is enabled to adapt to the culture requirements of different material conveying; the second box is after moving to suitable position, releases the pivot, and spacing spring promotes the stopper toward the outward movement, makes the stopper pulling connect the rope and removes, and the pivot is automatic to be rotated under connecting the rope drive, makes stopper and the automatic cooperation that forms of second spout inner wall, plays fixed action with the driving piece cooperation to the second box, promotes the sealed cooperation effect of second box and first box.

A rotating disc 242 is arranged at one end of the rotating shaft 24, a notch is formed in the rotating disc 242, a fifth sliding groove is formed in the side wall of the second box body 2, a third sliding block is arranged in the fifth sliding groove, and a limiting plate 25 matched with the notch is arranged on the third sliding block; the rotating shaft is easier to rotate under the arrangement of the rotating disc, so that the limiting block is convenient to control; when the position of the second box body is adjusted, the rotary disc is rotated, the connecting rope is wound on the rotating shaft to drive the limiting block to move towards the second movable cavity, then the limiting plate is inserted into the notch, and the rotary disc is fixed, so that the position of the second box body is adjusted under the action of the driving piece; after the position of second box is confirmed, take out the limiting plate from the breach, drive pivot autogiration under limiting spring's effect, make the limiting block contact with second spout inner wall, insert the limiting plate in the breach afterwards, play the fixed action to the carousel under the limiting plate effect, prevent that the carousel from rotating to drive the stopper and remove, guarantee the cooperation effect of limiting block and second spout.

A through cavity 222 is formed in the first slider 22, an air inlet nozzle is arranged at the top of the through cavity 222, the air inlet nozzle has the same structure as that of an air inlet nozzle of a bicycle tire in the prior art, and details are not repeated herein, first grooves are respectively formed in two ends of the through cavity 222, the sealing element is an air bag 223 arranged in the first grooves, and the air bag 223 is communicated with the through cavity 222; the air bag is made of latex; after the position of second box is confirmed, fill into the air from the suction nozzle department toward leading to the intracavity, make the air enter into the gasbag in, make the gasbag swell under the effect of atmospheric pressure and produce deformation, contact with second spout inner wall behind the gasbag volume grow, stop up the gap department of first slider and second spout, avoid external air to enter into the incubator in, mutually support with the stopper and play the fixed action to first slider, guarantee the cooperation effect of first box and second box.

The bottom of the first box body 1 is provided with a cavity, a bearing cavity assembly is arranged in the cavity, the bearing cavity assembly comprises a top plate 51, a bottom plate 52 and a sealing film 56, the top plate 51 and the bottom plate 52 are connected through a first connecting rod 55, the number of the first connecting rods 55 is multiple, every two first connecting rods 55 are arranged in a crossed mode to form a group of supporting assemblies, one end of each first connecting rod 55 is hinged to the top plate 51, and the other end of each first connecting rod 55 is hinged to the bottom plate 52; the side wall of the bottom plate is provided with a push block, so that the bottom plate can be conveniently pulled out of the cavity; when the bearing plate needs to be taken out of the incubator, the bearing cavity assembly is pulled out of the cavity, the bearing cavity is arranged on one side of the first sealing door, the top plate is pulled upwards, the bottom plate is separated from the top plate, the first connecting rod provides supporting force for the top plate, the cavity is formed between the bottom plate and the top plate, carbon dioxide is filled between the bottom plate and the top plate, environmental parameters in the bearing cavity are adjusted, various indexes in the bearing cavity are the same as those in the incubator, then the bearing plate is pushed into the bearing cavity, and the bearing cavity has excessive effect on taking of the bearing plate, so that the condition that the incubator is directly opened to enable air to enter the incubator to affect the culture environment in the incubator is avoided; because the space of the adapting cavity is small, the regulation and control of the environment in the adapting cavity are easy to regulate and control the environment in the culture cavity again, so that the culture cavity is in a relatively stable environment, and the survival rate of cell culture is improved; the bearing cavity assembly is stored through the cavity, so that the bearing cavity assembly is placed in the cavity when not in use, the space occupied by the culture cavity is reduced, and the use of the culture box is more convenient; the seal membrane top links firmly on the roof, and the seal membrane bottom is equipped with the hoop, is equipped with the montant on the bottom plate, and the hoop cover on the seal membrane is located on the montant, makes the seal membrane be connected with bottom plate and roof respectively.

The sealing film 56 is made of rubber, a plurality of supporting springs 54 are arranged on the bottom plate 52, a connecting film 53 is arranged on the side wall of the bottom plate 52, the connecting film 53 is made of rubber, and the other end of the connecting film 53 is fixedly connected to the top plate 51; the connecting film and the sealing film seal three surfaces of the bearing cavity, and one surface of the bearing cavity, which is in contact with the sealing door, is in a conduction state, so that the bearing plate can conveniently enter the bearing cavity; the supporting spring has a supporting function on the top plate, after the bearing cavity assembly is taken out of the cavity, the top plate is pushed to move upwards under the action of the supporting spring, so that the top plate and the bottom plate are separated, the connecting film has a sealing function on the side wall of the bearing cavity, the bearing cavity forms a sealed space, and the environment in the bearing cavity is conveniently controlled; through elastic material's setting, make seal membrane and connection membrane receive tensile when the roof removes, make connecting plate and seal membrane be connected with roof and bottom plate all the time, play good sealed effect to accepting the chamber.

A fourth movable cavity is arranged in the first sealing door 3, a second sealing door 32 is arranged in the fourth movable cavity, a plurality of first through grooves matched with the bearing plate 21 are formed in the first sealing door 3, a second through groove matched with the first through grooves is formed in the second sealing door 32, a first baffle 31 is arranged in each first through groove, and a second baffle 33 is arranged in each second through groove; cylinder groups used for controlling the opening and closing of the first baffle and the second baffle are arranged in the first sealing door and the second sealing door respectively, the number of the first through grooves corresponds to that of the first sliding grooves, and the first through grooves are arranged on one side of the first sliding grooves; when the second sealing door is arranged in the fourth movable cavity, the first through groove and the second through groove are aligned, when one bearing plate in the culture cavity needs to be taken, the bearing cavity assembly is taken out of the cavity, the bearing cavity assembly moves to one side of the corresponding bearing plate position, the environment in the bearing cavity is adjusted, the environment in the bearing cavity is the same as the environment in the culture cavity, the first baffle and the second baffle corresponding to one side of the bearing plate are opened, the bearing plate is pushed out from the first through groove and the second through groove, the bearing plate is arranged in the bearing cavity, when the bearing plate completely enters the bearing cavity, the first baffle and the second baffle are closed, the sealing membrane is opened, the bearing plate is taken out of the bearing cavity, cells on the bearing plate are picked up, and the influence of air directly entering the culture box to the environment in the culture box when one bearing plate is taken is avoided, ensure the steady state environment in the incubator and improve the survival rate of cells.

A fourth sliding block is arranged on the side wall of the top plate 51, a fifth sliding block is arranged on the side wall of the bottom plate 52, the fourth sliding block and the fifth sliding block have the same structure, a sixth sliding groove 34 matched with the fourth sliding block is arranged on the first sealing door 3, and a seventh sliding groove 35 matched with the fourth sliding block is arranged on the second sealing door 32; under the mutually supporting of slider and spout, the messenger accepts the chamber subassembly and can remove along the sealing door surface to make the sealing chamber subassembly move corresponding position department on the sealing door as required, be convenient for take out everywhere in the incubator accept the board, promote the convenience that the incubator used.

The top plate is provided with a sterilizing lamp, and the bottom plate is provided with a second groove matched with the sterilizing lamp.

Adjusting the position of the second box body according to the number of cells to be cultured, taking the second box body lifted to the top of the first box body as an example, taking the limiting plate out of the gap, rotating the turntable to enable the limiting block to enter the second movable cavity, and inserting the limiting plate into the gap; the push rod is pressed downwards, the air pressure rod pushes the first sliding block to move upwards, after the first sliding block moves to the top of the second sliding groove, the push rod is loosened, the limiting plate is taken out of the notch, the limiting spring pushes the limiting block to be in contact with the inner wall of the second sliding groove, after the limiting block is in contact with the inner wall of the second sliding groove, the limiting plate is inserted into the notch, air is introduced into the air inlet nozzle, the air is filled into the air bag and then is expanded to be in contact with the inner wall of the second sliding groove, and the joint of the first sliding block and the second sliding groove is sealed; putting cells to be cultured on the matched bearing plate, then pushing the bearing plate into the first chute and the third chute, extending the second sealing door out of the fourth movable cavity, covering the first sealing door and the second sealing door, sealing the incubator, controlling the environment in the incubator by the control panel to enable all indexes in the incubator to be in a standard state, and culturing the cells; when one bearing plate needs to be taken out, the bottom plate is pulled out of the cavity, the top plate is pushed to move upwards under the action of a limiting spring, the top plate and the bottom are matched with each other to form a bearing cavity, the bearing cavity is moved along the sliding groove to move to one side of the bearing plate needing to be taken out, the environment in the bearing cavity is adjusted to enable each index in the bearing cavity to be the same as the index in the incubator, the bearing plate is pushed out of the through groove under the action of the driving wheel after the baffle is opened, the bearing plate is pushed into the bearing cavity, after the bearing plate completely enters the bearing cavity, the baffle is closed, the sealing film is opened, the bearing plate is taken out of the bearing cavity, and the influence of air entering the incubator on cells on other bearing plates is avoided; when the bearing plate needs to be placed into the incubator again, the bearing plate is placed into the bearing cavity, the sealing film is closed, the environment in the bearing cavity is adjusted to enable the environment in the bearing cavity to be the same as that in the incubator, the sterilizing lamp is turned on to sterilize the bearing plate, the baffle is turned on to push the bearing plate into the through groove, the bearing plate enters the incubator under the action of the conveying wheel, and then the baffle is turned off; when accepting the chamber and not using the back, down promote and accept the chamber, will accept chamber propelling movement to cavity one side, push down the roof, make supporting spring be in compression state, with roof and bottom plate push into the cavity in, accept the chamber, reduce the shared space of incubator, the use of the incubator of being convenient for.

Claims

1. The utility model provides a carbon dioxide incubator for bioengineering, includes first box (1) and locates first sealing door (3) on the first box (1) lateral wall, first box (1) one side is equipped with equipment box (4), be equipped with display screen and operation panel on equipment box (4), its characterized in that: a second box body (2) is arranged in the first box body (1), a plurality of first sliding grooves are formed in the inner wall of the second box body (2), a bearing plate (21) is arranged in each first sliding groove, a second sliding groove (11) is formed in the inner wall of the first box body (1), a first sliding block (22) matched with the second sliding groove (11) is arranged on the side wall of the second box body (2), a driving piece used for pushing the first sliding block (22) to move upwards is arranged at the bottom of the second sliding groove (11), and a sealing piece is arranged on the side wall of the first sliding block (22);

a guide plate (14) is arranged at the bottom of the first box body (1), a first movable cavity matched with the guide plate (14) is arranged on the side wall of the second box body (2), and a plurality of third sliding grooves matched with the bearing plate (21) are formed in the inner wall of the guide plate (14);

a second movable cavity is arranged in the first sliding block (22), a limiting block (26) is arranged in the second movable cavity, a fourth sliding groove (221) is formed in the inner wall of the second movable cavity, a second sliding block (261) matched with the fourth sliding groove (221) is arranged on the side wall of the limiting block (26), a limiting spring (262) is arranged on the side wall of the second sliding block (261), and a rubber pad is arranged at one end of the limiting block (26);

a third movable cavity (23) is formed in the side wall of the second box body (2), the third movable cavity (23) is communicated with the second movable cavity, a through hole is formed in the side wall of the third movable cavity (23), a rotating shaft (24) penetrates through the through hole, the rotating shaft (24) is rotatably connected to the inner wall of the third movable cavity (23), an annular groove (241) is formed in the rotating shaft (24), a connecting rope is wound on the annular groove (241), and one end of the connecting rope is arranged on the limiting block (26);

a rotating disc (242) is arranged at one end of the rotating shaft (24), a notch is formed in the rotating disc (242), a fifth sliding groove is formed in the side wall of the second box body (2), a third sliding block is arranged in the fifth sliding groove, and a limiting plate (25) matched with the notch is arranged on the third sliding block;

a through cavity (222) is formed in the first sliding block (22), an air inlet nozzle is arranged at the top of the through cavity (222), first grooves are formed in two ends of the through cavity (222), the sealing element is an air bag (223) arranged in the first grooves, and the air bag (223) is communicated with the through cavity (222);

the bottom of the first box body (1) is provided with a cavity, a bearing cavity assembly is arranged in the cavity and comprises a top plate (51), a bottom plate (52) and a sealing film (56), the top plate (51) and the bottom plate (52) are connected through a first connecting rod (55), the number of the first connecting rods (55) is multiple, every two first connecting rods (55) are arranged in a crossed mode to form a group of supporting assemblies, one end of each first connecting rod (55) is hinged to the top plate (51), and the other end of each first connecting rod (55) is hinged to the bottom plate (52);

the sealing film (56) is made of elastic materials, a plurality of supporting springs (54) are arranged on the bottom plate (52), a connecting film (53) is arranged on the side wall of the bottom plate (52), the connecting film (53) is made of elastic materials, and the other end of the connecting film (53) is fixedly connected to the top plate (51);

a fourth movable cavity is arranged in the first sealing door (3), a second sealing door (32) is arranged in the fourth movable cavity, a plurality of first through grooves matched with the bearing plate (21) are formed in the first sealing door (3), a second through groove matched with the first through grooves is formed in the second sealing door (32), a first baffle (31) is arranged in the first through groove, and a second baffle (33) is arranged in the second through groove;

the novel sealing door is characterized in that a fourth sliding block is arranged on the side wall of the top plate (51), a fifth sliding block is arranged on the side wall of the bottom plate (52), the fourth sliding block is identical to the fifth sliding block in structure, a sixth sliding groove (34) matched with the fourth sliding block is formed in the first sealing door (3), and a seventh sliding groove (35) matched with the fourth sliding block is formed in the second sealing door (32).