CN209243075U - Isolated cells pressure-loaded experimental provision - Google Patents
Isolated cells pressure-loaded experimental provision Download PDFInfo
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- CN209243075U CN209243075U CN201821632597.1U CN201821632597U CN209243075U CN 209243075 U CN209243075 U CN 209243075U CN 201821632597 U CN201821632597 U CN 201821632597U CN 209243075 U CN209243075 U CN 209243075U
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- pressure
- loaded
- isolated cells
- chip microcontroller
- experimental provision
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Abstract
The utility model patent is related to the structure-design technique field of molecular biology experiment device, and in particular to a kind of isolated cells pressure-loaded experimental provision.The isolated cells pressure loading device can carry out pressure-loaded to isolated cells under the specified conditions, carry out quantitative control to the size of on-load pressure, acting frequency and acting duration etc..The device mainly includes three pressure-loaded modules, barometric surveying module and data collection and display module parts.The device controls pulse frequency and servo motor by single-chip microcontroller and the linear distance of piston motion is driven to realize to being loaded into the frequency of pressure on cell, size, the regulation of duration to meet different requirement of experiment, baroceptor can acquire the barometric information in cell incubator and pass to single-chip microcontroller, and barometric information can be acquired after single-chip microcontroller is handled and be come out by LCD display circuit real-time display.
Description
Technical field
The utility model relates to the structure-design technique fields of molecular biology experiment device, and in particular to a kind of in vitro thin
Born of the same parents' pressure-loaded experimental provision.
Background technique
Cyto-mechanics is the basis of cellular engineering and organizational engineering.The morphosis and its function of cell, cell
Growth, development, maturation, proliferation, aging, death and canceration, the differentiation and its Regulation Mechanism of cell are all special with the mechanics of cell
Property is related.It since cell individual is small, is visually difficult to observe, the thickness of cell membrane is even more there was only Nano grade, can not directly be made
With conventional macroscopic Mechanical loading method and experimental technique.To the effect of the cell applied force of in vitro culture to simulate cell in vivo
Force environment is the important method of the matter of utmost importance that cyto-mechanics faces and cyto-mechanics research.
The present Research of the domestic and international molecular biology experiment device of comprehensive analysis, the real physiological status of simulation cell and reality
The narrow research direction of experiment device is main Research Challenges.The utility model devises a kind of easy to operate, precise control, property
Isolated cells can be carried out pressure-loaded by the stable isolated cells pressure-loaded experimental provision of energy under the specified conditions, right
Size, acting frequency and acting duration of on-load pressure etc. carry out quantitative control.
Utility model content
To solve the above problems, the utility model devises a kind of isolated cells pressure-loaded experimental provision, the skill of use
Art scheme is: the experimental provision includes 3 pressure-loaded module, barometric surveying module and data collection and display module parts,
Wherein pressure-loaded module is made of the closed environment that cell incubator and piston are constituted;Barometric surveying module includes air pressure sensing
Device, amplifying circuit, A/D converter circuit and single-chip microcontroller;Data collection and display module includes single-chip microcontroller, data acquisition circuit and LCD aobvious
Show circuit.
The technical solution of the utility model is also: the device passes through that single-chip microcontroller controls pulse frequency and servo motor driving is lived
The linear distance of movement is filled in, is realized to being loaded into the frequency of pressure on cell, size, the regulation of duration.
The technical solution of the utility model is also: the device is by changing the closed loop being made of cell incubator and piston
The air pressure in domestic portion applies uniform positive/negative pressure to isolated cells.
The technical solution of the utility model is also: the device controls the operation of each module by single-chip microcontroller, acquires air pressure transmission
The barometric information of sensor transmitting is simultaneously come out it by LCD display circuit real-time display.
Compared with prior art, the utility model has the following beneficial effects: the device passes through control pulse frequency and servo electricity
Machine drives the linear distance of piston motion, realizes to being loaded into the frequency of pressure on cell, size, the regulation of duration, with
Meet different requirement of experiment.The pressure-loaded mode that the device uses makes cell uniform force, carries for transmitted load Loading Method
Lotus transmitting is easy and the bonding state independent of culture medium and culture dish.The device controls the fortune of each module by single-chip microcontroller
It goes, is not interfere with each other between each section, experimentation is set according to specific needs.
Detailed description of the invention
Fig. 1 is the overall structure figure of the utility model isolated cells pressure-loaded experimental provision.
Fig. 2 is the Facad structure figure of the utility model isolated cells pressure-loaded experimental provision.
In figure, 1. servo motors;2. piston;3. cell incubator;301. exhaust valve;302. cell culture chamber doors;303.
Glass partition;4. Tissue Culture Dish;5. baroceptor.
Specific embodiment
With reference to the accompanying drawings and examples, specific embodiment of the present utility model is described in further detail, it is real below
It applies example to be used to illustrate the utility model, but is not intended to limit the scope of the present invention.
As shown in Figure 1, the utility model isolated cells pressure-loaded experimental provision, each component part is respectively servo motor
1, piston 2, cell incubator 3, exhaust valve 301, cell culture chamber door 302, glass partition 303, Tissue Culture Dish 4, air pressure transmission
Sensor 5.
Illustrate the specific implementation process of isolated cells pressure-loaded experimental provision below with reference to Fig. 1 Fig. 2.
Before experiment starts, all devices need to be sterilized through high temperature and UV double.By baroceptor 5, amplifying circuit, AD
The hardware circuits such as conversion circuit, single-chip microcontroller, data acquisition circuit, LCD display circuit connect.
Exhaust valve 301 is opened, servo motor 1 is controlled by single-chip microcontroller, piston 2 is advanced in cell incubator 3, at this time
Exhaust valve 301 is turned off manually, cell culture chamber door 301 is opened, by vitro culture, well-grown cell and its Tissue Culture Dish
4 are put on glass partition 303, close cell culture chamber door 301, form stable closed environment in cell incubator 3.Monolithic
Machine controls servo motor 1 and piston 2 is driven to move back and forth, and makes to form the variable periodical air pressure of frequency, size in cell incubator 3
Variation, so that the cell in cell incubator 3 be made to be acted on by corresponding pressure.
When the air pressure in cell incubator 3 changes, baroceptor 5 can be by the barometric information of cell incubator 3
Single-chip microcontroller is passed to, single-chip microcontroller will be come out air pressure real-time display in incubator by LCD display circuit, while barometric information will
It can be collected by data acquisition circuit, so as to subsequent analysis result and probe into pressure to the influence of cell.
Claims (4)
1. isolated cells pressure-loaded experimental provision, it is characterised in that: the experimental provision includes pressure-loaded module, barometric surveying
3 parts of module and data collection and display module, wherein pressure-loaded module is made of closed cell incubator and piston
Environment composition;Barometric surveying module includes baroceptor, amplifying circuit, A/D converter circuit and single-chip microcontroller;Data acquisition display
Module includes single-chip microcontroller, data acquisition circuit and LCD display circuit.
2. isolated cells pressure-loaded experimental provision according to claim 1, it is characterised in that: the device passes through single-chip microcontroller
The linear distance for controlling pulse frequency and servo motor driving piston motion is realized to being loaded into the frequency of pressure on cell, big
Small, the duration regulation.
3. isolated cells pressure-loaded experimental provision according to claim 1, it is characterised in that: the device by change by
The air pressure inside closed environment that cell incubator and piston are constituted applies uniform positive/negative pressure to isolated cells.
4. isolated cells pressure-loaded experimental provision according to claim 1, it is characterised in that: the device passes through single-chip microcontroller
The operation of each module is controlled, the barometric information of acquisition baroceptor transmitting is simultaneously gone out it by LCD display circuit real-time display
Come.
Priority Applications (1)
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CN201821632597.1U CN209243075U (en) | 2018-10-09 | 2018-10-09 | Isolated cells pressure-loaded experimental provision |
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CN201821632597.1U CN209243075U (en) | 2018-10-09 | 2018-10-09 | Isolated cells pressure-loaded experimental provision |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113136326A (en) * | 2021-06-01 | 2021-07-20 | 赵昳 | Cell force application device |
CN113801791A (en) * | 2021-03-26 | 2021-12-17 | 中国海洋大学 | Multichannel uniform tensile stress in-vitro cell culture device and working method |
WO2022012607A1 (en) * | 2020-07-17 | 2022-01-20 | 上海我武干细胞科技有限公司 | Incubator |
-
2018
- 2018-10-09 CN CN201821632597.1U patent/CN209243075U/en not_active Expired - Fee Related
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022012607A1 (en) * | 2020-07-17 | 2022-01-20 | 上海我武干细胞科技有限公司 | Incubator |
CN115720592A (en) * | 2020-07-17 | 2023-02-28 | 上海我武干细胞科技有限公司 | Culture box |
JP2023510026A (en) * | 2020-07-17 | 2023-03-10 | 上海我武幹細胞科技有限公司 | incubator |
JP7368633B2 (en) | 2020-07-17 | 2023-10-24 | 上海我武幹細胞科技有限公司 | incubator |
CN113801791A (en) * | 2021-03-26 | 2021-12-17 | 中国海洋大学 | Multichannel uniform tensile stress in-vitro cell culture device and working method |
CN113801791B (en) * | 2021-03-26 | 2022-08-02 | 中国海洋大学 | Multichannel uniform tensile stress in-vitro cell culture device and working method |
CN113136326A (en) * | 2021-06-01 | 2021-07-20 | 赵昳 | Cell force application device |
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CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190813 Termination date: 20191009 |
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CF01 | Termination of patent right due to non-payment of annual fee |