CN210065732U - Experimental device for establish blast injury cell model - Google Patents
Experimental device for establish blast injury cell model Download PDFInfo
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- CN210065732U CN210065732U CN201920708836.5U CN201920708836U CN210065732U CN 210065732 U CN210065732 U CN 210065732U CN 201920708836 U CN201920708836 U CN 201920708836U CN 210065732 U CN210065732 U CN 210065732U
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Abstract
The utility model relates to an experimental device for establish explosion injury cell model, including rings, lifting rope, lifting hook, gallows, explode source rope, cell culture board support and cell culture board. The cell culture plate support comprises a support rod, a fixing frame, a butterfly screw and a matched nut, the upper end of the cell culture plate support is welded on the hanging frame, and the lower end of the cell culture plate support is connected and fixed with a cell culture plate. Compared with the prior art, the experimental device is simple to manufacture, three different explosion impact pressure groups can be carried out in one experiment, and the research of eight samples in each group is convenient to operate; the established blast injury cell model has high accuracy, strong controllability and good repeatability.
Description
Technical Field
The utility model relates to a cell model founds technical field, especially relates to an experimental apparatus of establishing blast injury cell model.
Background
In recent years, blast injury has become a hotspot for military medical research. The high-temperature and high-pressure gas generated after explosion compresses surrounding media to form shock waves which are transmitted from an explosion source to the outside in a radial mode, and the shock waves act on various organs and systems of a human body to cause injuries of different degrees and categories. The air blast shock wave pressure change can be expressed by the Friedlander equation, i.e., a momentarily elevated overpressure that subsequently decays exponentially below atmospheric pressure, ending when equilibrated with ambient pressure, where the low pressure phase duration is approximately twice the duration of the overpressure phase. The explosive shock wave transfers energy to the human body on its propagation path, and the degree of injury of the human body depends on five factors: first, an initial overpressure peak; second, the duration of the overpressure; thirdly, a medium for propagating the explosive shock wave; fourthly, the distance between the object and the explosion source; fifthly, the amplification effect of the relatively closed space is realized. When the blast wave acts on the human body, not only primary physical injury can be caused, but also a series of pathophysiological changes of tissues and cells in the body can be caused, such as oxidative stress, inflammatory reaction, blood brain barrier damage, nerve functional disorder and the like, so that the injury of the body is further aggravated.
The conventional experimental study on blast injury mainly utilizes an animal model, reconstructs an explosion scene at a physical level by determining physical parameters of explosion shock waves and simulates the damage of the explosion to an organism. However, the reaction of the body after the blast injury is a very complicated pathophysiological process, and various factors can influence the occurrence and development of the injury, and at this time, the animal model cannot accurately further study and analyze the cascade reaction between cells and reveal the pathophysiological change of tissue cells after the blast injury, so the blast injury needs to be studied and discussed from the cellular molecular level. However, at present, no experimental device specially used for establishing an explosive cell model is available, which brings inconvenience to research on the level of explosive cells. In order to better study the occurrence mechanism and the pathophysiological change of the blast injury, provide a basis for the treatment of the blast injury, and establish a standard and accurate blast injury cell model, which has important significance.
Disclosure of Invention
An object of the utility model is to provide an experimental apparatus for establish blast injury cell model to the not enough of existence on the prior art.
The purpose of the utility model can be realized by the following technical scheme:
an experimental device for establishing an explosion injury cell model comprises a lifting ring, a lifting rope, a lifting hook, a lifting frame, an explosion source rope, a cell culture plate bracket and a cell culture plate. The cell culture plate support is characterized in that the lifting ring is connected with the lifting hook through a lifting rope, the lifting hook is welded on the lifting frame, the explosion source rope is fixed on the lifting ring, the upper end of the cell culture plate support is welded on the lifting frame, and the lower end of the cell culture plate support is connected with a fixed cell culture plate.
Further, the hanging bracket is made of 304 stainless steel materials and comprises three circular supports including an outer ring support, a middle support and an inner ring support, and four connecting fixing rods which are arranged on the upper side, the lower side, the left side and the right side, wherein the outer ring support, the middle support, the inner ring support and the connecting fixing rods are arranged on the same plane, one end, the middle end and the other end of each connecting fixing rod are respectively welded on the outer ring support, the middle support and the inner ring support, the upper connecting fixing rods and the lower connecting fixing rods are arranged on the same straight line, the left connecting fixing rods and the right connecting fixing rods are arranged on the same straight line, and.
Preferably, the diameters of the outer ring frame, the middle frame and the inner ring frame can be adjusted according to experimental needs, and the lengths of the four connecting and fixing rods can also be adjusted according to the diameters of the outer ring frame, the middle frame and the inner ring frame.
Furthermore, the lifting hook is made of 304 stainless steel materials and comprises four lifting hooks which are welded at the joints of the four connecting fixing rods and the middle frame of the support respectively.
Furthermore, the lifting rope comprises four lifting hooks, the upper end of the lifting rope is fixed on the lifting ring, the lower end of the lifting rope is connected to the four lifting hooks, and the distances from the four lifting hooks to the lifting ring are equal.
Furthermore, the upper end of the explosion source rope is fixed on the hanging ring, the lower end of the explosion source rope is connected with an explosion source, the straight line of the explosion source rope is perpendicular to the plane of the hanging frame, and the position point of the explosion source is on the same plane with the center of the cell culture plate.
Furthermore, the cell culture plate support is made of 304 stainless steel materials and comprises support rods, a fixing frame, butterfly screws and matched nuts, wherein the fixing frame is welded at the lower ends of the support rods, the upper ends of the support rods are welded on an outer ring support, a middle support and an inner ring support of the support, the number of the support rods is 8, the included angle between the welding position of the adjacent support rods on the outer ring support and the circle center of the outer ring support is 45 degrees, the included angle between the welding position of the adjacent support rods on the inner ring support and the circle center of the middle support is 45 degrees, the included angle between the welding position of the support rods on the outer ring support and the circle center of the outer ring support and the included angle between the welding position of the support rods on the most adjacent middle support and the circle center of the middle support are 15 degrees, the welding position of the support rods on the middle support and the straight line on the circle center of the most adjacent middle support and the circle center of the inner ring support are 15 degrees, and the welding position of the circle center of And an included angle of the straight line is 15 degrees, and the butterfly screw and the matched nut form screw and nut connection on the fixing frame.
Furthermore, the cell culture plates are 96-well plates, 24 in total, are respectively installed on the cell culture plate support, are connected and fixed through screws and nuts formed by the butterfly screws and the matched nuts, and the front surface of the cell culture plate faces to the central explosion source.
Preferably, the cell culture plate support may be provided with a pressure sensor for measuring pressure and time variation of the position of the cell culture plate during explosion.
The utility model discloses the beneficial technological effect who has:
the experimental device for establishing the blast cell model of the utility model realizes the establishment of the blast cell model by using simple experimental design; the cell culture plate is fixed at three different distances from an explosion source through three circular supports with different diameters, cell research under different explosion impact pressures is realized, cell research of eight samples under the same explosion impact pressure is realized by using eight cell culture plate supports on each circular support, and by using the experimental device, three different explosion impact pressure groups can be carried out in one experiment, and the research of eight samples in each group is convenient to operate and good in repeatability; the cell culture plates under the adjacent three circular supports with different diameters are not on the same straight line, so that the influence of the cell culture plates close to the explosion source on the cell culture plates at a distance is avoided, and the experimental accuracy is high. Compared with the prior art, the experimental device is simple to manufacture and convenient to operate; the established blast injury cell model has high accuracy, strong controllability and good repeatability.
Drawings
Fig. 1 is a schematic structural diagram of an experimental device for establishing an explosion injury cell model of the present invention.
Fig. 2 is a top view of the experimental apparatus for establishing an explosion injury cell model of the present invention.
Fig. 3 is a schematic diagram of the cell culture plate support structure of the experimental device for establishing an explosion injury cell model.
Description of reference numerals: 1. a hoisting ring; 2. a lifting rope; 3. a hook; 4. a hanger; 4-1, outer ring frames; 4-2, a middle frame; 4-3, inner ring frame; 4-4, connecting a fixed rod; 5. a detonation source rope; 6. a cell culture plate support; 6-1, supporting rods; 6-2, fixing frames; 6-3, butterfly screws; 6-4, matching nuts; 7. cell culture plates.
Detailed Description
The invention will be further explained with reference to the drawings and examples.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be taken as limiting the scope of the invention.
As shown in FIGS. 1-3, an experimental device for establishing an explosion injury cell model comprises a lifting ring 1, a lifting rope 2, a lifting hook 3, a lifting frame 4, an explosion source rope 5, a cell culture plate bracket 6 and a cell culture plate 7. The cell culture plate lifting device is characterized in that the lifting ring 1 is connected with a lifting hook 3 through a lifting rope 2, the lifting hook 3 is welded on a lifting frame 4, an explosion source rope 5 is fixed on the lifting ring 1, the upper end of a cell culture plate support 6 is welded on the lifting frame 4, and the lower end of the cell culture plate support is connected with a fixed cell culture plate 7.
The hanging bracket 4 is made of 304 stainless steel and comprises three circular brackets, namely an outer ring bracket 4-1, a middle bracket 4-2 and an inner ring bracket 4-3, and four connecting fixing rods 4-4, namely an upper connecting fixing rod, a lower connecting fixing rod, a left connecting fixing rod and a right connecting fixing rod, wherein the outer ring bracket 4-1, the middle bracket 4-2, the inner ring bracket 4-3 and the connecting fixing rods 4-4 are arranged on the same plane, one end, the middle and the other end of each connecting fixing rod 4-4 are respectively welded on the outer ring bracket 4-1, the middle bracket 4-2 and the inner ring bracket 4-3, the upper connecting fixing rod 4-4 and the lower connecting fixing rod 4-4 are arranged on the same straight line, and the straight line where the upper connecting fixing rod 4-4 and the lower connecting fixing rod 4-. The diameters of the outer ring frame 4-1, the middle frame 4-2 and the inner ring frame 4-3 can be adjusted according to experimental needs, and the lengths of the four connecting and fixing rods 4-4 can also be adjusted according to the diameters of the outer ring frame 4-1, the middle frame 4-2 and the inner ring frame 4-3.
The lifting hook 3 is made of 304 stainless steel materials and comprises an upper part, a lower part, a left part and a right part which are respectively welded at the joints of the upper part, the lower part, the left part and the right part, which are connected with the fixing rods 4-4, and the middle frame 4-2 of the bracket.
The lifting rope 2 comprises four lifting hooks 3, the upper end of the lifting rope is fixed on the lifting ring 1, the lower end of the lifting rope is connected to the four lifting hooks 3, and the distances from the four lifting hooks 3 to the lifting ring 1 are equal.
The upper end of the explosion source rope 5 is fixed on the hanging ring 1, the lower end of the explosion source rope is connected with an explosion source, the straight line of the explosion source rope 5 is perpendicular to the plane of the hanging bracket 4, and the position point of the explosion source is on the same plane with the center of the cell culture plate 7.
The cell culture plate bracket 6 is made of 304 stainless steel materials and comprises a support rod 6-1, a fixing frame 6-2, a butterfly screw 6-3 and a matched nut 6-4, the fixing frame 6-2 is welded at the lower end of the support rod 6-1, the upper end of the support rod 6-1 is welded on an outer ring bracket 4-1, an intermediate bracket 4-2 and an inner ring bracket 4-3 of the bracket 4, 8 of the support rods are arranged, the included angle between the welding position of the adjacent support rod 6-1 on the outer ring bracket 4-1 and the circle center of the outer ring bracket 4-1 is 45 degrees, the included angle between the welding position of the adjacent support rod 6-1 on the intermediate bracket 4-2 and the circle center of the intermediate bracket 4-2 is 45 degrees, and the included angle between the welding position of the adjacent support rod 6-1 on the inner ring bracket 4-3 and the circle center of, the included angle between the welding position of the support rod 6-1 on the outer ring frame 4-1 and the straight line of the circle center of the outer ring frame 4-1 and the welding position of the support rod 6-1 on the middle frame 4-2 which is most adjacent and the straight line of the circle center of the middle frame 4-2 is 15 degrees, the included angle between the welding position of the support rod 6-1 on the middle frame 4-2 and the straight line of the circle center of the middle frame 4-2 and the welding position of the support rod 6-1 on the inner ring frame 4-3 which is most adjacent and the straight line of the circle center of the inner ring frame 4-3 is 15 degrees, and the butterfly screw 6-3 and the matched nut 6-4 form screw nut connection on the fixed frame 6-2.
The cell culture plates 7 are 96 pore plates, the number of the cell culture plates is 24, the cell culture plates are respectively arranged on the cell culture plate bracket 6, the butterfly screws 6-3 and the matched nuts 6-4 form screw nuts for connection and fixation, and the front surfaces of the cell culture plates 7 face to the central explosion source. The cell culture plate support 6 may be provided with a pressure sensor for measuring the pressure and time variation at the location of the cell culture plate 7 during explosion.
The working principle of the utility model comprises the following steps:
step 1, connecting a lifting ring 1 and a lifting hook 3 by using a lifting rope 2, fixing an explosion source rope 5, and suspending a lifting frame 4 in an experimental site by using the lifting ring 1 to complete installation preparation of an experimental device in the early stage of an experiment;
and 2, selecting a proper cell line to be researched, and culturing the cells in an incubator by using a corresponding culture medium. Cells were cultured 24 hours before the explosion by transferring to a 96-well cell culture plate 7, 15000 cells per well, preferably the middle culture well. On the day of explosion, the cell culture medium in the corresponding culture well of the cell culture plate 7 was replaced to avoid the generation of air bubbles. Sealing the cell culture plate 7 with a sterile air-permeable polyester film before explosion, and fixing the edge of the sterile air-permeable polyester film on the cell culture plate 7 with a sterile adhesive tape;
step 3, placing the cell culture plate 7 on a corresponding cell culture plate bracket 6, quickly fixing by using a butterfly screw 6-3 and a matched nut 6-4, installing an explosion source and an explosion cable, and then exploding to perform explosion impact;
and 4, immediately taking down the cell culture plate 7 after explosion, removing the sterile breathable polyester film, placing the cell culture plate in an incubator for incubation, and then carrying out the next research.
The experimental device of the utility model is simple to manufacture, three different explosion impact pressure groups can be carried out in one experiment, and the research of eight samples in each group is convenient to operate; the established blast injury cell model has high accuracy, strong controllability and good repeatability.
The above is a detailed description of the experimental apparatus for establishing the blast injury cell model of the present invention, and it should be noted that the present invention is not limited to the above specific embodiments. Various modifications and changes may be made by those skilled in the art without departing from the principles of the invention, which is intended to be within the scope of the invention as defined by the appended claims.
Claims (9)
1. The utility model provides an experimental apparatus of blasting cell model is established which characterized in that: including rings (1), lifting rope (2), lifting hook (3), gallows (4), explosion source rope (5), cell culture board support (6) and cell culture board (7), rings (1) are connected with lifting hook (3) through lifting rope (2), lifting hook (3) welding is on gallows (4), explosion source rope (5) are fixed on rings (1), welding is on gallows (4) on cell culture board support (6), and fixed cell culture board (7) are connected to the lower extreme.
2. The experimental device for establishing the blast injury cell model according to claim 1, wherein: the hanging bracket (4) is made of 304 stainless steel materials and comprises three circular brackets of an outer ring bracket (4-1), a middle bracket (4-2) and an inner ring bracket (4-3), and four connecting fixing rods (4-4) which are arranged at the upper part, the lower part, the left part and the right part, wherein the outer ring frame (4-1), the middle frame (4-2), the inner ring frame (4-3) and the connecting fixing rods (4-4) are arranged on the same plane, one end, the middle and the other end of the connecting fixing rod (4-4) are respectively welded on the outer ring frame (4-1), the middle frame (4-2) and the inner ring frame (4-3), the upper and lower connecting fixing rods (4-4) are on the same straight line, the left and right connecting fixing rods (4-4) are on the same straight line, the straight line where the upper and lower connecting fixing rods (4-4) are located is perpendicular to the straight line where the left and right connecting fixing rods (4-4) are located.
3. The experimental device for establishing the blast injury cell model according to claim 2, wherein: the diameters of the outer ring frame (4-1), the middle frame (4-2) and the inner ring frame (4-3) can be adjusted according to experimental needs, and the lengths of the four connecting fixing rods (4-4) can also be adjusted according to the diameters of the outer ring frame (4-1), the middle frame (4-2) and the inner ring frame (4-3).
4. The experimental device for establishing the blast injury cell model according to claim 1, wherein: the lifting hook (3) is made of 304 stainless steel materials and comprises an upper part, a lower part, a left part and a right part which are respectively welded at the joints of the upper part, the lower part, the left part and the right part of the connecting fixing rod (4-4) and the middle frame (4-2) of the bracket.
5. The experimental device for establishing the blast injury cell model according to claim 1, wherein: the lifting rope (2) comprises four lifting hooks (3) which are vertically and horizontally arranged, the upper end of the lifting rope is fixed on the lifting ring (1), the lower end of the lifting rope is connected to the four lifting hooks (3) which are vertically and horizontally arranged, and the distances from the four lifting hooks (3) to the lifting ring (1) are equal.
6. The experimental device for establishing the blast injury cell model according to claim 1, wherein: the upper end of the explosion source rope (5) is fixed on the hanging ring (1), the lower end of the explosion source rope is connected with an explosion source, the straight line of the explosion source rope (5) is perpendicular to the plane of the hanging bracket (4), and the position point of the explosion source and the center of the cell culture plate (7) are on the same plane.
7. The experimental device for establishing the blast injury cell model according to claim 1, wherein: the cell culture plate support (6) is made of 304 stainless steel and comprises support rods (6-1), a fixing frame (6-2), butterfly screws (6-3) and matched nuts (6-4), the fixing frame (6-2) is welded at the lower end of each support rod (6-1), the upper ends of the support rods (6-1) are welded on an outer ring frame (4-1), a middle frame (4-2) and an inner ring frame (4-3) of the support (4) respectively in 8 numbers, the included angle between the welding position of the adjacent support rod (6-1) on the outer ring frame (4-1) and the circle center of the outer ring frame (4-1) is 45 degrees, the included angle between the welding position of the adjacent support rod (6-1) on the middle frame (4-2) and the circle center of the middle frame (4-2) is 45 degrees, and the welding position of the adjacent support rod (6-1) on the inner ring frame (4-3) and the circle center The included angle of the circle centers of the frames (4-3) is 45 degrees, the included angle of the welding position of the supporting rod (6-1) on the outer ring frame (4-1) and the straight line of the circle center of the outer ring frame (4-1) and the straight line of the welding position of the supporting rod (6-1) on the middle frame (4-2) which is most adjacent to the welding position of the circle center of the outer ring frame and the straight line of the circle center of the middle frame (4-2) is 15 degrees, the included angle between the welding position of the supporting rod (6-1) on the middle frame (4-2) and the straight line of the circle center of the middle frame (4-2) and the closest welding position of the supporting rod (6-1) on the inner ring frame (4-3) and the straight line of the circle center of the inner ring frame (4-3) is 15 degrees, the butterfly screw (6-3) and the matched nut (6-4) form screw and nut connection on the fixing frame (6-2).
8. The experimental apparatus for establishing the blast injury cell model according to claim 7, wherein: the cell culture plates (7) are 96-well plates, the number of the cell culture plates is 24, the cell culture plates are respectively arranged on the cell culture plate bracket (6), the butterfly screws (6-3) and the matched nuts (6-4) form screw and nut connection and fixation, and the front surfaces of the cell culture plates (7) face to the center explosion source.
9. The experimental device for establishing the blast injury cell model according to claim 1, wherein: the cell culture plate bracket (6) can be provided with a pressure sensor for measuring the pressure and time change of the position of the cell culture plate (7) during explosion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920708836.5U CN210065732U (en) | 2019-05-17 | 2019-05-17 | Experimental device for establish blast injury cell model |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920708836.5U CN210065732U (en) | 2019-05-17 | 2019-05-17 | Experimental device for establish blast injury cell model |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210065732U true CN210065732U (en) | 2020-02-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920708836.5U Expired - Fee Related CN210065732U (en) | 2019-05-17 | 2019-05-17 | Experimental device for establish blast injury cell model |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210065732U (en) |
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2019
- 2019-05-17 CN CN201920708836.5U patent/CN210065732U/en not_active Expired - Fee Related
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200214 Termination date: 20210517 |
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| CF01 | Termination of patent right due to non-payment of annual fee |