CN110024913A - One primary yeast tunning and the compound of polyglutamic acid and the preparation method and application thereof - Google Patents
One primary yeast tunning and the compound of polyglutamic acid and the preparation method and application thereof Download PDFInfo
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- CN110024913A CN110024913A CN201910278496.1A CN201910278496A CN110024913A CN 110024913 A CN110024913 A CN 110024913A CN 201910278496 A CN201910278496 A CN 201910278496A CN 110024913 A CN110024913 A CN 110024913A
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- 108010020346 Polyglutamic Acid Proteins 0.000 title claims abstract description 56
- 229920002643 polyglutamic acid Polymers 0.000 title claims abstract description 56
- 150000001875 compounds Chemical class 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 244000285963 Kluyveromyces fragilis Species 0.000 title claims abstract description 11
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 title claims abstract description 11
- 235000013336 milk Nutrition 0.000 claims abstract description 56
- 239000008267 milk Substances 0.000 claims abstract description 56
- 210000004080 milk Anatomy 0.000 claims abstract description 56
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 49
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 22
- 239000008103 glucose Substances 0.000 claims abstract description 20
- 239000004094 surface-active agent Substances 0.000 claims abstract description 15
- 239000004459 forage Substances 0.000 claims abstract description 13
- 239000012064 sodium phosphate buffer Substances 0.000 claims abstract description 13
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims abstract description 12
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 12
- 239000011734 sodium Substances 0.000 claims abstract description 12
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 11
- 239000012531 culture fluid Substances 0.000 claims abstract description 11
- 238000011218 seed culture Methods 0.000 claims abstract description 11
- 239000001888 Peptone Substances 0.000 claims abstract description 6
- 108010080698 Peptones Proteins 0.000 claims abstract description 6
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims abstract description 6
- 235000013923 monosodium glutamate Nutrition 0.000 claims abstract description 6
- 235000019319 peptone Nutrition 0.000 claims abstract description 6
- 239000000047 product Substances 0.000 claims description 24
- 239000000203 mixture Substances 0.000 claims description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 19
- 230000001376 precipitating effect Effects 0.000 claims description 11
- 238000005119 centrifugation Methods 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 10
- 238000001556 precipitation Methods 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 7
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 5
- 229920000053 polysorbate 80 Polymers 0.000 claims description 5
- 239000002244 precipitate Substances 0.000 claims description 2
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims 1
- 229940066779 peptones Drugs 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 6
- 210000004767 rumen Anatomy 0.000 abstract description 6
- 229940073490 sodium glutamate Drugs 0.000 abstract description 5
- 230000000392 somatic effect Effects 0.000 abstract description 5
- 238000000354 decomposition reaction Methods 0.000 abstract description 3
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract description 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 3
- 238000000926 separation method Methods 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 235000001727 glucose Nutrition 0.000 abstract 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 241000283690 Bos taurus Species 0.000 description 38
- 238000002474 experimental method Methods 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 241000235342 Saccharomycetes Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000003115 biocidal effect Effects 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 241000282849 Ruminantia Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000012228 culture supernatant Substances 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 210000001082 somatic cell Anatomy 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 235000019728 animal nutrition Nutrition 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 210000005075 mammary gland Anatomy 0.000 description 1
- 208000004396 mastitis Diseases 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 230000036642 wellbeing Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Sustainable Development (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Birds (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
One primary yeast tunning and the compound of polyglutamic acid and the preparation method and application thereof, belong to field of biotechnology.The compound is made of yeast tunning and polyglutamic acid.The method is as follows: dry ferment being added in sodium phosphate buffer and is mixed, then glucose and surfactant is added, yeast tunning extract is obtained after enzymatic hydrolysis separation, concentration, hay bacillus seed culture fluid will be added after the sterilizing of sodium glutamate, glucose and peptone, polyglutamic acid sodium is obtained after enzymatic hydrolysis separation, concentration, then mixes yeast extractive from fermentative and polyglutamic acid sodium.Product of the present invention can be improved the quantity of microorganism in cow rumen after mixing with ration, milk cow is promoted to absorb the decomposition of forage nutrient with this, enhance milk cow physical fitness, to make the core index in quality examination, somatic number in milk has obtained effective control, 24 percent is had dropped, risk of falling ill is reduced, the output of milk of milk cow is made to be significantly improved.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to the compound of a primary yeast tunning and polyglutamic acid and its
Preparation method and application.
Background technique
In recent years, by microorganism formulation adjust microbial population of animal intestinal tract, maintain microecological balance, improve immunity of organisms with
And various physiological functions are paid close attention to by numerous researchers always, are one of the hot spots of Animal nutrition research.Saccharomycete is one
Kind single celled eukaryotic microorganism, its breeding cycle is short, and culture is easy and yield is high.Not only contain in yeast somatic cells and depends on
Propylhomoserin, while also vitamin and microelement rich in etc..
There are many it is demonstrated experimentally that addition saccharomycete can be with the function of cud in Effective Regulation ruminant in daily ration
Can, promote nutrient absorption, improves immunity.Saccharomycete can be effectively improved stable state mistake with other enteric microorganism collective effects
The rumen microorganism activity of weighing apparatus, takes in amount of dry matter so as to cause milk cow and milk cow increases to the digestibility of feed, milk
The output of milk and milk production quality of ox are also improved.The type of influence of the saccharomycete to ruminant tumor gastric and saccharomycete itself,
Quality, ruminant physiological status and type of food grain etc. have relationship.
All the time, what traditional feed additive used is all antibiotic, but nowadays, antibiotic is exposed various
Drawback is more and more.In contrast, then more obvious the advantages of ferment compound: nontoxic, pollution-free, do not generate resistance.Lotus this
After the feed for having eaten addition ferment compound, yeast ferments smooth milk cow in the cud of ox, promotes the decomposition of cellulose, has
Nutrition is absorbed conducive to animal, the utilization rate of lactic acid is improved, helps to prevent rumen ecology, improves micro- life in enteron aisle
The living environment of object.
But the damage and inflammation of mammary gland are be easy to cause in domestic raising dairy cattle and milking operation at present, cause milk cow by
The output of milk is seriously affected in inflammatory problems, therefore, manufacturer has to using antibiotic, and antibiotic usage will be good for the mankind
Health causes damages, and in addition also will affect the cud and intestinal flora of milk cow, absorption of the milk cow to nutriment is degrading, into evil
Property circulation.
Summary of the invention
The technical issues of solution: in view of the above technical problems, the present invention provides a primary yeast tunning and polyglutamic acid
Compound and the preparation method and application thereof, the product and milk cow forage of preparation blend, milk cattle rumen fermentation mode can be regulated and controled,
It reduces ferment compound and adds quantity, improve milk yield and milk quality, obtain higher economic benefit.
Technical solution: the compound of a primary yeast tunning and polyglutamic acid, the compound is by yeast tunning
It is formed with polyglutamic acid, wherein the mass ratio of yeast tunning and polyglutamic acid is (1~5): 1.
The preparation method of the compound of above-mentioned yeast tunning and polyglutamic acid, the preparation method include following step
It is rapid:
Dry ferment is added in sodium phosphate buffer and mixes by step 1, and glucose and surfactant is then added,
In, the ratio of dry ferment and sodium phosphate buffer is 50g:0.8L, and the ratio of glucose and surfactant is 200g:5mL, Portugal
The mass ratio of grape sugar and dry ferment be 4:1, then by mixture after 28-37 DEG C, 180-250r/min shaking table culture 8h 100
20min is heated at a temperature of DEG C, centrifugation removal precipitating precipitates supernatant with 2 times of volume 99vt.% alcohol, then by wine
In 60-80 DEG C of baking oven overnight, the product of drying is crushed to obtain yeast extractive from fermentative essence precipitating products therefrom；
Step 2 sterilizes the culture medium containing 50g/L sodium glutamate, 40g/L glucose and 10g/L peptone
Afterwards, hay bacillus seed culture fluid is added at a temperature of 32 DEG C, wherein the volume ratio of hay bacillus seed culture fluid and culture medium
For 3mL:1L, then mixture is cultivated on 28-37 DEG C, 180-250r/min shaking table for 24 hours, with centrifugation removal after the completion of culture
Precipitating, supernatant is precipitated with 2 times of volume 99vt.% alcohol, then by alcohol precipitation products therefrom in 60-80 DEG C of baking oven
In overnight, the product of drying is crushed to obtain polyglutamic acid sodium；
Step 3 mixes the polyglutamic acid sodium of yeast extractive from fermentative and step 2 preparation prepared by step 1.
Preferably, it is 5.5-6.5, molal weight 0.5mol-0.6mol that pH, which is added, in dry ferment in the step 1
It is mixed in sodium phosphate buffer.
Preferably, surfactant is Tween 80 surfactant in the step 1.
The compound of above-mentioned yeast extractive from fermentative and polyglutamic acid is preparing the application in milk cow forage.
Preferably, basic ration is matched in the compound and milk cow forage of the yeast extractive from fermentative and polyglutamic acid
Than for 1:1000-1:5000.
The utility model has the advantages that compound of the present invention by addition yeast extractive from fermentative and polyglutamic acid in milk cow forage,
It can be improved the quantity of microorganism in cow rumen, promote milk cow to absorb the decomposition of forage nutrient with this, enhance milk cow body
Quality has dropped 24 percent, reduces risk of falling ill so that the somatic number in milk be made to have obtained effective control,
The output of milk of milk cow is set to be increased to 29 kg/days from 27.67 kg/days of averagely every ox, somatic number is dropped from 37/mL ten thousand
As low as 280,000/mL.If yeast extractive from fermentative, which is used alone, the output of milk of somatic number and milk cow in control milk
Certain to improve, the output of milk has been increased to 28.2 kg/days from 27.4 kg/days and has been increased to, and somatic number is reduced from 37/mL ten thousand
To 320,000/mL, but it is unobvious that polyglutamic acid effect is used alone.
Daily every cow head need to only use the compound of 15g the primary yeast extractive from fermentative and polyglutamic acid, can make every
Cow head monthly increases about 1.2-1.5 kilograms of the output of milk.This measure not only reduces milk cow forage addition quantity, reduces up-front investment
Cost, while milk production efficiency is substantially increased, and since this kind of method is easily operated, it is suitable for extensive milk cow livestock-raising,
Considerable economic well-being of workers and staff will be brought for milk cattle cultivating.
Detailed description of the invention
Fig. 1 is each group milk cow average milk production statistical chart weekly described in the embodiment of the present invention 1.
Specific embodiment
The invention will be further described in the following with reference to the drawings and specific embodiments.
Embodiment 1
The compound of one primary yeast tunning and polyglutamic acid, the compound is by yeast tunning and polyglutamic acid
Composition, wherein the mass ratio of yeast tunning and polyglutamic acid is 3:1.
The preparation method of the compound of above-mentioned yeast tunning and polyglutamic acid, the preparation method include following step
It is rapid:
Step 1 by dry ferment be added pH be 6.0, molal weight be 0.5mol sodium phosphate buffer in mix, then plus
Enter glucose and Tween 80 surfactant, wherein the ratio of dry ferment and sodium phosphate buffer be 50g:0.8L, glucose and
The ratio of surfactant is 200g:5mL, and the mass ratio of glucose and dry ferment is 4:1, then by mixture 30 DEG C,
20min, centrifugation removal precipitating, by 2 times of volumes of supernatant are heated after 220r/min shaking table culture 8h at a temperature of 100 DEG C
99vt.% alcohol is precipitated, and is then stayed overnight alcohol precipitation products therefrom in 80 DEG C of baking ovens, the product of drying is crushed
Obtain yeast extractive from fermentative；
Step 2 sterilizes the culture medium containing 50g/L sodium glutamate, 40g/L glucose and 10g/L peptone
Afterwards, hay bacillus seed culture fluid is added at a temperature of 32 DEG C, wherein the volume ratio of hay bacillus seed culture fluid and culture medium
For 3mL:1L, then mixture is cultivated on 32 DEG C, 220r/min shaking table for 24 hours, it, will with centrifugation removal precipitating after the completion of culture
Supernatant is precipitated with 2 times of volume 99vt.% alcohol, then stays overnight alcohol precipitation products therefrom in 80 DEG C of baking ovens, is dried
Dry product is crushed to obtain polyglutamic acid sodium；
Step 3 mixes the polyglutamic acid sodium of yeast extractive from fermentative and step 2 preparation prepared by step 1.
The compound of yeast tunning and polyglutamic acid, including yeast tunning and polyglutamic acid.It can not only improve
Milk yield, and cow recessive mastitis can also be reduced, milk cow production performance is had an important influence.Then pass through certain skill
Art means are merged under the premise of guaranteeing its physiological activity, compound state preparation are made.During the experiment, behaviour can be simplified
Make, directly mixes it with basic ration, and feeding effect is more preferable than the effect for feeding basic ration, can make cow producing milk
Amount is obviously improved, while somatic cell count is declined, and improves the diversity and stability of milk cow intestinal flora.
By the way that the technological means such as fresh yeast lotion (enzymatic hydrolysis) separation, concentration are obtained pure natural yeast tunning, and
Other composite parts for being conducive to cow rumen microbial fermentation are added on this basis, include various essential amino acids, dimension life
Element, nucleotide, polypeptide and microelement.Ferment compound is mixed with polyglutamic acid, it is every that its appropriate addition is blended in milk cow
In its ration, the raising of milk production of cow and quality is only realized by way of daily nursing.
The products application of preparation is tested in milk cow, experimental subjects is 20 Chinese Holstein milk in the present embodiment
Ox, in experiment periods, selected experiment ox is in lactation mid-term, and the indexs such as their age, parity and milk composition are all close,
It is randomly divided into two groups.
Wherein, first group is control group, is fed with conventional feed；Second group is experimental group, and experimental group is in control group tradition
The yeast tunning of 15g/ (top margin of a page) and the compound of polyglutamic acid are added on the basis of feed.A length of 30 days when experiment,
7 days preliminary experiments have been carried out before formal test.During experiment, timing measures the daily yielding and body cell of 20 cow heads daily
Several concrete conditions.Concrete outcome is concluded that according to interpretation of result and is compared with control group referring to following table and Fig. 1
It was found that feed 30 days of experimental group milk cattle fooder addition mixture, the output of milk significantly improves 4.02%.It is possible thereby to prove,
The compound of yeast tunning and polyglutamic acid increases significantly the effect of Milk Production to china holstein cows.
Embodiment 2
The compound of one primary yeast tunning and polyglutamic acid, the compound is by yeast tunning and polyglutamic acid
Composition, wherein the mass ratio of yeast tunning and polyglutamic acid is 1:1.
The preparation method of the compound of above-mentioned yeast tunning and polyglutamic acid, the preparation method include following step
It is rapid:
Step 1 by dry ferment be added pH be 5.5, molal weight be 0.5mol sodium phosphate buffer in mix, then plus
Enter glucose and Tween 80 surfactant, wherein the ratio of dry ferment and sodium phosphate buffer be 50g:0.8L, glucose and
The ratio of surfactant is 200g:5mL, and the mass ratio of glucose and dry ferment is 4:1, then by mixture 28 DEG C,
20min, centrifugation removal precipitating, by 2 times of volumes of supernatant are heated after 180r/min shaking table culture 8h at a temperature of 100 DEG C
99vt.% alcohol is precipitated, and is then stayed overnight alcohol precipitation products therefrom in 60 DEG C of baking ovens, the product of drying is crushed
Obtain yeast extractive from fermentative；
Step 2 sterilizes the culture medium containing 50g/L sodium glutamate, 40g/L glucose and 10g/L peptone
Afterwards, hay bacillus seed culture fluid is added at a temperature of 32 DEG C, wherein the volume ratio of hay bacillus seed culture fluid and culture medium
For 3mL:1L, then mixture is cultivated on 28 DEG C, 180r/min shaking table for 24 hours, it, will with centrifugation removal precipitating after the completion of culture
Supernatant is precipitated with 2 times of volume 99vt.% alcohol, then stays overnight alcohol precipitation products therefrom in 60 DEG C of baking ovens, is dried
Dry product is crushed to obtain polyglutamic acid sodium；
Step 3 mixes the polyglutamic acid sodium of yeast extractive from fermentative and step 2 preparation prepared by step 1.
The compound of above-mentioned yeast tunning and polyglutamic acid is preparing the application in milk cow forage.The yeast fermentation
The proportion of basic ration is 1:1000 in the compound and milk cow forage of product and polyglutamic acid.
Embodiment 3
The compound of one primary yeast tunning and polyglutamic acid, the compound is by yeast tunning and polyglutamic acid
Composition, wherein the mass ratio of yeast tunning and polyglutamic acid is 5:1.
The preparation method of the compound of above-mentioned yeast tunning and polyglutamic acid, the preparation method include following step
It is rapid:
Step 1 by dry ferment be added pH be 6.5, molal weight be 0.6mol sodium phosphate buffer in mix, then plus
Enter glucose and Tween 80 surfactant, wherein the ratio of dry ferment and sodium phosphate buffer be 50g:0.8L, glucose and
The ratio of surfactant is 200g:5mL, and the mass ratio of glucose and dry ferment is 4:1, then by mixture 37 DEG C,
20min, centrifugation removal precipitating, by 2 times of volumes of supernatant are heated after 250r/min shaking table culture 8h at a temperature of 100 DEG C
99vt.% alcohol is precipitated, and is then stayed overnight alcohol precipitation products therefrom in 80 DEG C of baking ovens, the product of drying is crushed
Obtain yeast extractive from fermentative；
Step 2 sterilizes the culture medium containing 50g/L sodium glutamate, 40g/L glucose and 10g/L peptone
Afterwards, hay bacillus seed culture fluid is added at a temperature of 32 DEG C, wherein the volume ratio of hay bacillus seed culture fluid and culture medium
For 3mL:1L, then mixture is cultivated on 37 DEG C, 250r/min shaking table for 24 hours, it, will with centrifugation removal precipitating after the completion of culture
Supernatant is precipitated with 2 times of volume 99vt.% alcohol, then stays overnight alcohol precipitation products therefrom in 80 DEG C of baking ovens, is dried
Dry product is crushed to obtain polyglutamic acid sodium；
Step 3 mixes the polyglutamic acid sodium of yeast extractive from fermentative and step 2 preparation prepared by step 1.
The compound of above-mentioned yeast tunning and polyglutamic acid is preparing the application in milk cow forage.The yeast fermentation
The proportion of basic ration is 1:5000 in the compound and milk cow forage of product and polyglutamic acid.
Claims (6)
1. the compound of a primary yeast tunning and polyglutamic acid, which is characterized in that the compound is by yeast tunning
It is formed with polyglutamic acid, wherein the mass ratio of yeast tunning and polyglutamic acid is (1 ~ 5): 1.
2. the preparation method of the compound of yeast tunning described in claim 1 and polyglutamic acid, which is characterized in that described
Preparation method the following steps are included:
Dry ferment is added in sodium phosphate buffer and mixes by step 1, and glucose and surfactant is then added, wherein dry
The ratio of yeast and sodium phosphate buffer is 50 g:0.8 L, and the ratio of glucose and surfactant is 200 g:5 mL, Portugal
The mass ratio of grape sugar and dry ferment be 4:1, then by mixture after 28-37 DEG C, 8 h of 180-250 r/min shaking table culture
20 min are heated at a temperature of 100 DEG C, centrifugation removal precipitating precipitates supernatant with 2 times of 99 vt.% alcohol of volume, then
In 60-80 DEG C of baking oven overnight by alcohol precipitation products therefrom, the product of drying is crushed to obtain yeast extractive from fermentative；
After step 2 is sterilized the culture medium containing 50 g/L sodium glutamates, 40 g/L glucose and 10 g/L peptones,
Hay bacillus seed culture fluid is added at a temperature of 32 DEG C, wherein the volume ratio of hay bacillus seed culture fluid and culture medium is 3
Then mixture is cultivated 24 h by mL:1 L on 28-37 DEG C, 180-250 r/min shaking table, with centrifugation removal after the completion of culture
Precipitating, supernatant is precipitated with 2 times of 99 vt.% alcohol of volume, then by alcohol precipitation products therefrom in 60-80 DEG C of baking oven
In overnight, the product of drying is crushed to obtain polyglutamic acid sodium；
Step 3 mixes the polyglutamic acid sodium of yeast extractive from fermentative and step 2 preparation prepared by step 1.
3. the preparation method of the compound of yeast tunning according to claim 2 and polyglutamic acid, which is characterized in that
It is 5.5-6.5 that pH, which is added, in dry ferment in the step 1, and molal weight is to mix in 0.5 mol-0.6 mol sodium phosphate buffer
It is even.
4. the preparation method of the compound of yeast tunning according to claim 2 and polyglutamic acid, which is characterized in that
Surfactant is Tween 80 surfactant in the step 1.
5. the compound of yeast tunning described in claim 1 and polyglutamic acid is preparing the application in milk cow forage.
6. application according to claim 5, which is characterized in that the compound of the yeast tunning and polyglutamic acid with
The proportion of basic ration is 1:1000-1:5000 in milk cow forage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910278496.1A CN110024913A (en) | 2019-04-09 | 2019-04-09 | One primary yeast tunning and the compound of polyglutamic acid and the preparation method and application thereof |
Applications Claiming Priority (1)
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| US20110003035A1 (en) * | 2008-03-18 | 2011-01-06 | Xuefeng Yu | Yeast composition and its use as cow feed additive |
| CN101999525A (en) * | 2010-11-02 | 2011-04-06 | 河北科技大学 | Composite probiotics feed additive |
| CN102499331A (en) * | 2011-12-28 | 2012-06-20 | 内蒙古伊利实业集团股份有限公司 | Dairy cow rumen regulating agent and preparation method thereof |
| CN106666129A (en) * | 2017-01-06 | 2017-05-17 | 北京中农弘科生物技术有限公司 | Application of yeast culture in preparing feed for improving production performance of lactating dairy cow |
| CN108841882A (en) * | 2018-07-26 | 2018-11-20 | 武琳慧 | A method of thallus fermenting and producing polyglutamic acid is discarded using glutamic acid fermentation |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110003035A1 (en) * | 2008-03-18 | 2011-01-06 | Xuefeng Yu | Yeast composition and its use as cow feed additive |
| CN101999525A (en) * | 2010-11-02 | 2011-04-06 | 河北科技大学 | Composite probiotics feed additive |
| CN102499331A (en) * | 2011-12-28 | 2012-06-20 | 内蒙古伊利实业集团股份有限公司 | Dairy cow rumen regulating agent and preparation method thereof |
| CN106666129A (en) * | 2017-01-06 | 2017-05-17 | 北京中农弘科生物技术有限公司 | Application of yeast culture in preparing feed for improving production performance of lactating dairy cow |
| CN108841882A (en) * | 2018-07-26 | 2018-11-20 | 武琳慧 | A method of thallus fermenting and producing polyglutamic acid is discarded using glutamic acid fermentation |
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