CN109963948B - 用于定量扩增的方法 - Google Patents

用于定量扩增的方法 Download PDF

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Publication number
CN109963948B
CN109963948B CN201780032103.8A CN201780032103A CN109963948B CN 109963948 B CN109963948 B CN 109963948B CN 201780032103 A CN201780032103 A CN 201780032103A CN 109963948 B CN109963948 B CN 109963948B
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amplification
quantitative
sample
target
standard
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Chinese (zh)
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CN109963948A (zh
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U.K.斯保尔丁
M.罗加切瓦
M.A.波里茨
R.J.克里斯普
S.C.J.马罗
C.H.A.巴朗格
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Biomerieux SA
Biofire Defense LLC
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Biomerieux SA
Biofire Defense LLC
Biofire Diagnostics Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Evolutionary Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Steroid Compounds (AREA)
  • Algebra (AREA)
  • Saccharide Compounds (AREA)
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CN201780032103.8A 2016-03-24 2017-03-20 用于定量扩增的方法 Active CN109963948B (zh)

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US201662313032P 2016-03-24 2016-03-24
US62/313032 2016-03-24
PCT/US2017/023151 WO2017165269A1 (en) 2016-03-24 2017-03-20 Methods for quantitative amplification

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US (2) US11268141B2 (https=)
EP (2) EP3433383B1 (https=)
JP (3) JP6979029B2 (https=)
CN (2) CN109963948B (https=)
AU (2) AU2017237983B2 (https=)
CA (1) CA3018187A1 (https=)
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WO (1) WO2017165269A1 (https=)

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EP3662480A4 (en) 2017-08-04 2021-05-19 BillionToOne, Inc. MOLECULES ASSOCIATED WITH A TARGET FOR A CHARACTERIZATION ASSOCIATED WITH BIOLOGICAL TARGETS
US11519024B2 (en) * 2017-08-04 2022-12-06 Billiontoone, Inc. Homologous genomic regions for characterization associated with biological targets
CN111655833A (zh) * 2017-11-13 2020-09-11 株式会社理光 检测判断装置
AU2018353924A1 (en) 2017-12-29 2019-07-18 Clear Labs, Inc. Automated priming and library loading device
US10597714B2 (en) 2017-12-29 2020-03-24 Clear Labs, Inc. Automated priming and library loading device
US10101328B1 (en) 2017-12-29 2018-10-16 Clear Labs, Inc. Detection of microorganisms in food samples and food processing facilities
WO2020072459A1 (en) * 2018-10-02 2020-04-09 Biofire Diagnostics, Llc Bacterial response
JP7499795B2 (ja) * 2019-07-30 2024-06-14 アリファックス ソチエタ レスポンサビリタ リミタータ 微生物を同定する方法およびシステム
EP4622974A1 (en) 2022-11-22 2025-10-01 bioMérieux New nucleic acid binding compounds and uses
WO2025141105A1 (en) 2023-12-27 2025-07-03 bioMérieux Specific probes for the detection of nucleic acids, methods and uses

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JP2024012410A (ja) 2024-01-30
JP7453953B2 (ja) 2024-03-21
CN109963948A (zh) 2019-07-02
EP4137583A1 (en) 2023-02-22
EP3433383B1 (en) 2022-11-16
US20190078134A1 (en) 2019-03-14
AU2023202560B2 (en) 2025-02-13
AU2017237983B2 (en) 2023-02-23
AU2023202560A1 (en) 2023-05-18
ES2936823T3 (es) 2023-03-22
WO2017165269A1 (en) 2017-09-28
EP3433383A4 (en) 2019-10-02
JP6979029B2 (ja) 2021-12-08
US11268141B2 (en) 2022-03-08
JP2019509051A (ja) 2019-04-04
CA3018187A1 (en) 2017-09-28
CN115786472A (zh) 2023-03-14
JP2022028789A (ja) 2022-02-16
US20210254133A1 (en) 2021-08-19
AU2017237983A1 (en) 2018-10-18
EP3433383A1 (en) 2019-01-30

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