CA3018187A1 - Methods for quantitative amplification - Google Patents
Methods for quantitative amplification Download PDFInfo
- Publication number
- CA3018187A1 CA3018187A1 CA3018187A CA3018187A CA3018187A1 CA 3018187 A1 CA3018187 A1 CA 3018187A1 CA 3018187 A CA3018187 A CA 3018187A CA 3018187 A CA3018187 A CA 3018187A CA 3018187 A1 CA3018187 A1 CA 3018187A1
- Authority
- CA
- Canada
- Prior art keywords
- sample
- amplification
- quantification
- standard
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/26—Inoculator or sampler
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Bioinformatics & Computational Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Evolutionary Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Medical Informatics (AREA)
- Theoretical Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Steroid Compounds (AREA)
- Algebra (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- General Physics & Mathematics (AREA)
- Mathematical Analysis (AREA)
- Mathematical Optimization (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662313032P | 2016-03-24 | 2016-03-24 | |
| US62/313,032 | 2016-03-24 | ||
| PCT/US2017/023151 WO2017165269A1 (en) | 2016-03-24 | 2017-03-20 | Methods for quantitative amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3018187A1 true CA3018187A1 (en) | 2017-09-28 |
Family
ID=59899700
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3018187A Pending CA3018187A1 (en) | 2016-03-24 | 2017-03-20 | Methods for quantitative amplification |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US11268141B2 (https=) |
| EP (2) | EP3433383B1 (https=) |
| JP (3) | JP6979029B2 (https=) |
| CN (2) | CN109963948B (https=) |
| AU (2) | AU2017237983B2 (https=) |
| CA (1) | CA3018187A1 (https=) |
| ES (1) | ES2936823T3 (https=) |
| WO (1) | WO2017165269A1 (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11090652B2 (en) | 2016-07-28 | 2021-08-17 | Biofire Defense, Llc | Self-contained nucleic acid processing |
| EP3662480A4 (en) | 2017-08-04 | 2021-05-19 | BillionToOne, Inc. | MOLECULES ASSOCIATED WITH A TARGET FOR A CHARACTERIZATION ASSOCIATED WITH BIOLOGICAL TARGETS |
| US11519024B2 (en) * | 2017-08-04 | 2022-12-06 | Billiontoone, Inc. | Homologous genomic regions for characterization associated with biological targets |
| CN111655833A (zh) * | 2017-11-13 | 2020-09-11 | 株式会社理光 | 检测判断装置 |
| AU2018353924A1 (en) | 2017-12-29 | 2019-07-18 | Clear Labs, Inc. | Automated priming and library loading device |
| US10597714B2 (en) | 2017-12-29 | 2020-03-24 | Clear Labs, Inc. | Automated priming and library loading device |
| US10101328B1 (en) | 2017-12-29 | 2018-10-16 | Clear Labs, Inc. | Detection of microorganisms in food samples and food processing facilities |
| WO2020072459A1 (en) * | 2018-10-02 | 2020-04-09 | Biofire Diagnostics, Llc | Bacterial response |
| JP7499795B2 (ja) * | 2019-07-30 | 2024-06-14 | アリファックス ソチエタ レスポンサビリタ リミタータ | 微生物を同定する方法およびシステム |
| EP4622974A1 (en) | 2022-11-22 | 2025-10-01 | bioMérieux | New nucleic acid binding compounds and uses |
| WO2025141105A1 (en) | 2023-12-27 | 2025-07-03 | bioMérieux | Specific probes for the detection of nucleic acids, methods and uses |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6645758B1 (en) | 1989-02-03 | 2003-11-11 | Johnson & Johnson Clinical Diagnostics, Inc. | Containment cuvette for PCR and method of use |
| US6780617B2 (en) | 2000-12-29 | 2004-08-24 | Chen & Chen, Llc | Sample processing device and method |
| US6303305B1 (en) | 1999-03-30 | 2001-10-16 | Roche Diagnostics, Gmbh | Method for quantification of an analyte |
| US6691041B2 (en) | 2000-03-31 | 2004-02-10 | Roche Molecular Systems, Inc. | Method for the efficiency-corrected real-time quantification of nucleic acids |
| US7790368B1 (en) * | 2000-09-22 | 2010-09-07 | Hitachi, Ltd. | Method for analyzing nucleic acid |
| CA2450343C (en) | 2001-07-16 | 2013-12-17 | Idaho Technology, Inc. | Thermal cycling system and method of use |
| US20050014176A1 (en) | 2002-10-18 | 2005-01-20 | Swiger Roy R. | Method for quantitative end-point PCR |
| US7788039B2 (en) * | 2003-09-25 | 2010-08-31 | Roche Molecular Systems, Inc. | Quantitation of nucleic acids using growth curves |
| US7387887B2 (en) | 2004-04-20 | 2008-06-17 | University Of Utah Research Foundation | Nucleic acid melting analysis with saturation dyes |
| EP1920070B1 (en) | 2005-05-09 | 2019-08-07 | BioFire Diagnostics, LLC | Self-contained biological analysis |
| JP4936419B2 (ja) | 2005-06-03 | 2012-05-23 | 学校法人日本大学 | Ｄｎａの定量的検出方法 |
| JP2005348742A (ja) * | 2005-07-25 | 2005-12-22 | Hitachi Ltd | 核酸解析方法 |
| US7368246B2 (en) | 2005-09-15 | 2008-05-06 | Primera Biosystems, Inc. | Quantitative gene expression profiling |
| WO2007061284A1 (en) * | 2005-11-22 | 2007-05-31 | Plant Research International B.V. | Multiplex nucleic acid detection |
| US8895295B2 (en) | 2006-11-15 | 2014-11-25 | Biofire Diagnostics, Llc | High density self-contained biological analysis |
| WO2009061897A1 (en) * | 2007-11-07 | 2009-05-14 | Primera Biosystems, Inc. | Quantification of nucleic acid molecules using multiplex pcr |
| CN101619346B (zh) * | 2008-07-04 | 2014-02-19 | 上海交通大学医学院附属瑞金医院 | 人冠心病易感基因-脂蛋白a基因拷贝数变异检测方法和试剂盒 |
| WO2010104893A2 (en) | 2009-03-09 | 2010-09-16 | Life Technologies Corporation | Methods for the determination of a copy number of a genomic sequence in a biological sample |
| ES2605303T3 (es) | 2010-07-29 | 2017-03-13 | F. Hoffmann-La Roche Ag | Detección cualitativa y cuantitativa de ácidos nucleicos microbianos |
| JP6231989B2 (ja) | 2011-11-10 | 2017-11-15 | バイオファイア・ダイアグノスティクス，リミテッド・ライアビリティ・カンパニー | 充填バイアル |
| EP2855692B1 (en) | 2012-05-24 | 2019-07-24 | University of Utah Research Foundation | Extreme pcr |
| SG10201804101XA (en) * | 2012-09-10 | 2018-07-30 | Biofire Diagnostics Llc | Multiple amplification cycle detection |
| JP6479336B2 (ja) * | 2014-04-23 | 2019-03-06 | 国立研究開発法人産業技術総合研究所 | 微生物の１６ＳｒＲＮＡ遺伝子定量用内部標準遺伝子 |
| EP2990491B1 (en) * | 2014-08-28 | 2017-07-12 | F. Hoffmann-La Roche AG | Oligonucleotides for controlling amplification of nucleic acids |
| WO2017044694A2 (en) | 2015-09-11 | 2017-03-16 | The Board Of Trustrees Of The Leland Stanford Junior University | Method of determining the prognosis of hepatocellular carcinomas using a multigene signature associated with metastasis |
| US10267802B2 (en) | 2015-09-23 | 2019-04-23 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of prognosis and diagnosis of ovarian cancer |
-
2017
- 2017-03-20 CN CN201780032103.8A patent/CN109963948B/zh active Active
- 2017-03-20 EP EP17770890.6A patent/EP3433383B1/en active Active
- 2017-03-20 ES ES17770890T patent/ES2936823T3/es active Active
- 2017-03-20 AU AU2017237983A patent/AU2017237983B2/en active Active
- 2017-03-20 US US16/087,724 patent/US11268141B2/en active Active
- 2017-03-20 JP JP2018549796A patent/JP6979029B2/ja active Active
- 2017-03-20 CN CN202310030180.7A patent/CN115786472A/zh active Pending
- 2017-03-20 CA CA3018187A patent/CA3018187A1/en active Pending
- 2017-03-20 EP EP22199836.2A patent/EP4137583A1/en active Pending
- 2017-03-20 WO PCT/US2017/023151 patent/WO2017165269A1/en not_active Ceased
-
2021
- 2021-05-03 US US17/246,972 patent/US20210254133A1/en active Pending
- 2021-11-12 JP JP2021184574A patent/JP7453953B2/ja active Active
-
2023
- 2023-04-27 AU AU2023202560A patent/AU2023202560B2/en active Active
- 2023-10-27 JP JP2023184871A patent/JP2024012410A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| JP2024012410A (ja) | 2024-01-30 |
| JP7453953B2 (ja) | 2024-03-21 |
| CN109963948A (zh) | 2019-07-02 |
| EP4137583A1 (en) | 2023-02-22 |
| EP3433383B1 (en) | 2022-11-16 |
| US20190078134A1 (en) | 2019-03-14 |
| AU2023202560B2 (en) | 2025-02-13 |
| AU2017237983B2 (en) | 2023-02-23 |
| AU2023202560A1 (en) | 2023-05-18 |
| ES2936823T3 (es) | 2023-03-22 |
| WO2017165269A1 (en) | 2017-09-28 |
| EP3433383A4 (en) | 2019-10-02 |
| CN109963948B (zh) | 2023-01-24 |
| JP6979029B2 (ja) | 2021-12-08 |
| US11268141B2 (en) | 2022-03-08 |
| JP2019509051A (ja) | 2019-04-04 |
| CN115786472A (zh) | 2023-03-14 |
| JP2022028789A (ja) | 2022-02-16 |
| US20210254133A1 (en) | 2021-08-19 |
| AU2017237983A1 (en) | 2018-10-18 |
| EP3433383A1 (en) | 2019-01-30 |
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