CN115786472A - 用于定量扩增的方法 - Google Patents

用于定量扩增的方法 Download PDF

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Publication number
CN115786472A
CN115786472A CN202310030180.7A CN202310030180A CN115786472A CN 115786472 A CN115786472 A CN 115786472A CN 202310030180 A CN202310030180 A CN 202310030180A CN 115786472 A CN115786472 A CN 115786472A
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sample
quantitative
amplification
standard
target
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Pending
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CN202310030180.7A
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English (en)
Chinese (zh)
Inventor
U.K.斯保尔丁
M.罗加切瓦
M.A.波里茨
R.J.克里斯普
S.C.J.马罗
C.H.A.巴朗格
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Biomerieux SA
Biofire Defense LLC
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Biomerieux SA
Biofire Defense LLC
Biofire Diagnostics Inc
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Publication of CN115786472A publication Critical patent/CN115786472A/zh
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
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    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Steroid Compounds (AREA)
  • Amplifiers (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Algebra (AREA)
  • General Physics & Mathematics (AREA)
  • Mathematical Analysis (AREA)
  • Mathematical Optimization (AREA)
CN202310030180.7A 2016-03-24 2017-03-20 用于定量扩增的方法 Pending CN115786472A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662313032P 2016-03-24 2016-03-24
US62/313032 2016-03-24
CN201780032103.8A CN109963948B (zh) 2016-03-24 2017-03-20 用于定量扩增的方法
PCT/US2017/023151 WO2017165269A1 (en) 2016-03-24 2017-03-20 Methods for quantitative amplification

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US (2) US11268141B2 (https=)
EP (2) EP4137583A1 (https=)
JP (3) JP6979029B2 (https=)
CN (2) CN115786472A (https=)
AU (2) AU2017237983B2 (https=)
CA (1) CA3018187A1 (https=)
ES (1) ES2936823T3 (https=)
WO (1) WO2017165269A1 (https=)

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JP7010926B2 (ja) * 2016-07-28 2022-01-26 バイオファイア・ダイアグノスティクス，リミテッド・ライアビリティ・カンパニー 自己完結型の核酸処理
US11519024B2 (en) * 2017-08-04 2022-12-06 Billiontoone, Inc. Homologous genomic regions for characterization associated with biological targets
WO2019028462A1 (en) 2017-08-04 2019-02-07 Billiontoone, Inc. TARGET-ASSOCIATED MOLECULES FOR CHARACTERIZATION ASSOCIATED WITH BIOLOGICAL TARGETS
WO2019093530A1 (ja) * 2017-11-13 2019-05-16 株式会社リコー 検出判定用デバイス
US10597714B2 (en) 2017-12-29 2020-03-24 Clear Labs, Inc. Automated priming and library loading device
EP3731959B1 (en) 2017-12-29 2025-10-08 Clear Labs, Inc. Automated priming and library loading device
US10246704B1 (en) 2017-12-29 2019-04-02 Clear Labs, Inc. Detection of microorganisms in food samples and food processing facilities
WO2020072459A1 (en) * 2018-10-02 2020-04-09 Biofire Diagnostics, Llc Bacterial response
KR102763678B1 (ko) * 2019-07-30 2025-02-05 알리팍스 에스알엘 미생물을 동정하는 방법 및 시스템
CA3270929A1 (en) 2022-11-22 2024-05-30 bioMérieux NEW NUCLEIC ACID BINDING COMPOUNDS AND THEIR USES
WO2025141105A1 (en) 2023-12-27 2025-07-03 bioMérieux Specific probes for the detection of nucleic acids, methods and uses

Citations (5)

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Publication number Publication date
CN109963948A (zh) 2019-07-02
EP4137583A1 (en) 2023-02-22
JP7453953B2 (ja) 2024-03-21
JP6979029B2 (ja) 2021-12-08
EP3433383B1 (en) 2022-11-16
JP2022028789A (ja) 2022-02-16
AU2017237983B2 (en) 2023-02-23
CN109963948B (zh) 2023-01-24
US20210254133A1 (en) 2021-08-19
EP3433383A1 (en) 2019-01-30
ES2936823T3 (es) 2023-03-22
AU2023202560B2 (en) 2025-02-13
EP3433383A4 (en) 2019-10-02
JP2024012410A (ja) 2024-01-30
US11268141B2 (en) 2022-03-08
US20190078134A1 (en) 2019-03-14
CA3018187A1 (en) 2017-09-28
AU2023202560A1 (en) 2023-05-18
WO2017165269A1 (en) 2017-09-28
JP2019509051A (ja) 2019-04-04
AU2017237983A1 (en) 2018-10-18

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