CN109907037B - Constant temperature perfusion type isolated organ perfusion container - Google Patents
Constant temperature perfusion type isolated organ perfusion container Download PDFInfo
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- CN109907037B CN109907037B CN201910284008.8A CN201910284008A CN109907037B CN 109907037 B CN109907037 B CN 109907037B CN 201910284008 A CN201910284008 A CN 201910284008A CN 109907037 B CN109907037 B CN 109907037B
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Abstract
The invention relates to a constant-temperature perfusion type isolated organ perfusion container, which comprises: the liquid storage pot, the inside of liquid storage pot is separated and is formed first cavity and second cavity, first cavity is used for the holding perfusate, the second cavity is used for holding heat exchange liquid, the heat of heat exchange liquid is followed the second cavity transmits extremely in the first cavity among the perfusate, above-mentioned constant temperature perfusion formula isolated organ perfusion container can effectively keep the perfusate temperature to make isolated organ obtain effective the preservation.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a constant-temperature perfusion type isolated organ perfusion container.
Background
When organ transplantation is carried out, whether the isolated organ can be effectively preserved can directly determine the success or failure of the transplantation operation.
At present, the isolated organ usually can place on perfusion container and toward isolated organ in the perfusate to reach isolated organ's save and restoration purpose, along with the save time increases, the perfusate temperature of holding in perfusion container can descend gradually, when the perfusate temperature drops to being less than clinical experiment temperature, can cause isolated organ damage, leads to the isolated organ can't reach the transplantation standard.
Disclosure of Invention
In view of the above, there is a need to provide a constant-temperature perfusion type isolated organ perfusion container, so that the constant-temperature perfusion type isolated organ perfusion container can effectively maintain the temperature of perfusate, thereby effectively preserving the isolated organ.
In order to achieve the above object, the present invention provides a constant-temperature perfusion type isolated organ perfusion container, comprising: the liquid storage pot, the inside of liquid storage pot is separated and is formed first cavity and second cavity, first cavity is used for the holding perfusate, the second cavity is used for the holding heat exchange liquid, the heat of heat exchange liquid can be followed the second cavity transmits to in the first cavity in the perfusate.
Compared with the background technology, the constant-temperature perfusion type isolated organ perfusion container at least has the following beneficial effects: set up first cavity and second cavity in the liquid storage pot, the heat transfer of the heat exchange liquid in the second cavity is to first cavity to this perfusate in to first cavity heats, effectively avoids the temperature of perfusate to drop to clinical experiment temperature and causes the condition emergence of separation organ damage, thereby makes the separation organ obtain effective the saving.
In one embodiment, the second cavity is arranged around the outer peripheral wall of the first cavity, or the first cavity is arranged around the outer peripheral wall of the second cavity.
In one embodiment, the device further comprises a support film, the liquid storage tank is provided with a mounting hole, the support film is mounted on the mounting hole in a covering mode, the liquid storage tank is further provided with a liquid outlet used for communicating the first cavity with an isolated organ pipeline, and the support film is provided with a leakage hole used for communicating with the first cavity.
In one embodiment, a liquid adding port communicated with the first cavity is formed in the liquid storage tank, and a liquid discharging port communicated with the first cavity is formed in the liquid storage tank.
In one embodiment, the liquid storage tank is a transparent tank body, and a silk-screen printing portion for marking the volume of the perfusion liquid is arranged on the side wall of the liquid storage tank.
In one embodiment, the liquid storage tank is provided with a heat exchange inlet and a heat exchange outlet which are respectively communicated with the second cavity, and the heat exchange inlet and the heat exchange outlet are respectively connected with a heating device pipeline to form a heat exchange circulation passage.
In one embodiment, a temperature measuring port is arranged on the liquid storage tank, and a temperature detecting unit for detecting the temperature of the perfusate or the heat exchange liquid is arranged in the temperature measuring port.
In one embodiment, the heat exchange outlet is disposed at a lower portion of the second cavity, and the heat exchange inlet is disposed at an upper portion of the second cavity.
In one embodiment, an opening for communicating the second cavity with the outside is formed in the bottom of the liquid storage tank, and a bottom cover for covering the opening is detachably arranged on the liquid storage tank.
In one embodiment, the bottom cover is provided with a sealing member, and the sealing member is tightly pressed between the bottom cover and the outer edge of the opening.
Drawings
FIG. 1 is a front view of a constant temperature perfused isolated organ perfusion vessel according to an embodiment of the present invention;
FIG. 2 is a sectional view taken along line A-A of the perfusion vessel of FIG. 1;
FIG. 3 is a top view of the perfusion vessel of FIG. 1;
fig. 4 is a partial cross-sectional view of the thermostatically-perfused isolated organ perfusion container of fig. 1.
10. A liquid storage tank 11, a first cavity 12, a second cavity 13, a liquid outlet 14, a liquid filling opening 15, a liquid discharge opening 16, a heat exchange inlet 17, a heat exchange outlet 18, a temperature measuring opening 19, a bottom cover 191, a sealing element 20, a support film 21 and a leakage hole.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. In contrast, when an element is referred to as being "directly on" another element, there are no intervening elements present. The terms "first" and "second" used herein do not denote any particular order or quantity, but rather are used to distinguish one element from another.
As shown in fig. 1 and 2, a constant-temperature perfusion-type isolated organ perfusion container includes: liquid storage pot 10, the inside of liquid storage pot 10 is separated and is formed first cavity 11 and second cavity 12, and first cavity 11 is used for the holding perfusate, and second cavity 12 is used for the holding heat exchange liquid, and the heat of heat exchange liquid can be followed second cavity 12 and is transmitted to the perfusate in the first cavity 11.
Above-mentioned constant temperature perfusion type isolated organ perfusion container is through setting up first cavity 11 and second cavity 12 in liquid storage pot 10, and the heat transfer of the heat exchange fluid in the second cavity 12 is to first cavity 11 to this perfusate in first cavity 11 heats, compare with traditional constant temperature perfusion type isolated organ perfusion container, can effectively avoid the temperature of perfusate to drop to clinical experiment temperature and cause the condition emergence of isolated organ damage, thereby make the isolated organ obtain effective the preservation.
Preferably, the constant-temperature perfusion type isolated organ perfusion container is applicable to perfusion preservation of isolated lungs, particularly to perfusion preservation of isolated lungs of animals, and certainly, the constant-temperature perfusion type isolated organ perfusion container is also applicable to perfusion preservation of other isolated organs of human bodies or animals, and is not limited specifically herein.
Specifically, the heat exchange fluid may be selected from water or an antifreeze.
Further, referring to fig. 2, the second cavity 12 is disposed around the outer circumferential wall of the first cavity 11, or the first cavity 11 is disposed around the outer circumferential wall of the second cavity 12. Particularly, the periphery wall of first cavity 11 is located to the ring of second cavity 12 to increase heat transfer area, simultaneously, second cavity 12 surrounds the periphery of first cavity 11 and can play the heat retaining effect of perfusate in the first cavity 11, avoids the temperature decline of perfusate too fast, thereby it is long when the preservation of extension isolated organ. It should be noted that the relative position of the first cavity 11 and the second cavity 12 can be in various forms, as long as the side wall of the first cavity 11 contacts with the side wall of the second cavity 12 to realize heat transfer, and is not limited in detail herein.
In an embodiment, please refer to fig. 2 to 3, the constant-temperature perfusion type isolated organ perfusion container further includes a support film 20, a mounting hole (not shown in the drawings) is formed in the liquid storage tank 10, the support film 20 is covered and mounted on the mounting hole, a liquid outlet 13 for communicating the first cavity 11 with the isolated organ pipeline is further formed in the liquid storage tank 10, and a leak 21 for communicating with the first cavity 11 is formed in the support film 20. The isolated organ is placed on a support membrane 20. The contact surface of the container for holding the isolated organ is the supporting membrane 20, and the oppression feeling to the isolated organ such as the isolated lung is smaller than that of the hard material. The perfusate in the first cavity 11 flows into the isolated organ from the liquid outlet 13 along the pipeline, then flows out of the isolated organ, and then flows into the first cavity 11 again through the leak 21 on the support membrane 20, so as to realize the circular flow of the perfusate.
Specifically, with reference to fig. 2 and fig. 3, the mounting hole is disposed above the first cavity 11, that is, after the supporting membrane 20 covers the mounting hole, the supporting membrane 20 is located above the first cavity 11, and correspondingly, the perfusate flowing out from the isolated organ directly flows back into the first cavity 11 through the leaking hole.
Specifically, the support membrane 20 is a medical soft membrane, such as a silica gel membrane, so as to avoid damage to the isolated organ.
Furthermore, the supporting film 20 is recessed toward the direction close to the first cavity 11, so as to provide more accommodation space for the isolated organ, and avoid the isolated organ from being damaged due to compression when the isolated organ is stored.
Specifically, referring to fig. 3, a plurality of weep holes 21 are disposed on the support film 20 to accelerate the backflow speed of the perfusion fluid. Specifically, three leak holes 21 are provided, but of course, the number of leak holes 21 may be different according to actual needs, and is not particularly limited herein.
In an embodiment, referring to fig. 1, a liquid filling port 14 for communicating with the first cavity 11 is disposed on the liquid storage tank 10, and a liquid discharging port 15 for communicating with the first cavity 11 is disposed on the liquid storage tank 10. When the perfusate in first cavity 11 is not enough, accessible filling opening 14 adds the perfusate in first cavity 11, wherein, the mode of adding includes artifical mode and the automatic mode of adding, and in the automatic mode of adding, filling opening 14 passes through the pipeline and is connected with the fluid infusion device, and when the perfusate was not enough, the operable fluid infusion device was toward filling opening 14 input perfusate to the addition control accuracy when improving the interpolation perfusate. Port 14 may also be used to add medications. Meanwhile, in the process of perfusing the isolated organ, part of perfusate can be discharged from the liquid outlet 15, and new perfusate is added into the liquid adding port 14 to realize perfusate replacement. After the isolated organ perfusion is completed, the perfusate can be completely emptied from the liquid discharge port 15, so that the bacteria breeding caused by long-time storage of the perfusate in the first cavity 11 is avoided.
Further, the liquid storage tank 10 is a transparent tank body, and a silk-screen portion for marking the volume of the perfusion liquid is arranged on the side wall of the liquid storage tank 10. By observing the scale position of the silk-screen printing part where the liquid level of the perfusate is positioned, the real-time capacity of the perfusate can be intuitively obtained, so that the perfusate can be timely added.
Specifically, the fluid reservoir 10 may be selected from a transparent PMMA tank or a transparent PC tank.
In an embodiment, please refer to fig. 1 to 3, a heat exchange inlet 16 and a heat exchange outlet 17 are disposed on the liquid storage tank 10 and are respectively used for communicating with the second cavity 12, and the heat exchange inlet 16 and the heat exchange outlet 17 are respectively connected with a heating device pipeline to form a heat exchange circulation path. The heat exchange liquid flows back to the second cavity 12 through the heat exchange outlet 17, the heating device and the heat exchange inlet 16 in sequence, and the heat exchange liquid flows back to the second cavity 12 after being heated in the heating device so as to keep the temperature of the heat exchange liquid in the second cavity 12 constant, thereby more effectively ensuring that the perfusate reaches a temperature range required by a clinical experiment and further ensuring that the isolated organ is effectively stored.
Further, referring to fig. 1, a temperature measuring port 18 is disposed on the liquid storage tank 10, and a temperature detecting unit (not shown in the drawings) for detecting the temperature of the perfusate or the heat exchange fluid is disposed in the temperature measuring port 18. Particularly, the temperature detecting unit is used for detecting the temperature of the perfusate, and feeds back the temperature data to the controller, and the controller controls the power of the heating device according to the temperature data, so that the temperature of the heat exchange liquid is changed, the temperature of the perfusate is better controlled, and the temperature of the perfusate is guaranteed to be maintained in a temperature interval required by a clinical experiment.
Further, referring to fig. 2, the heat exchange outlet 17 is disposed at the lower portion of the second cavity 12, and the heat exchange inlet 16 is disposed at the upper portion of the second cavity 12, so as to better realize the heating circulation of the heat exchange liquid, and thus better realize the heat exchange.
In one embodiment, referring to fig. 1, 2 and 4, the bottom of the liquid storage tank 10 is provided with an opening (not shown) for communicating the second cavity 12 with the outside, and the liquid storage tank 10 is detachably provided with a bottom cover 19 for covering the opening. After the isolated organ perfusion is completed, the bottom cover 19 can be detached from the liquid storage tank 10, and the inside of the second cavity 12 is cleaned, so that the inside of the second cavity 12 is kept clean and sanitary, and the bacteria breeding is avoided. The detachable connection manner includes a plurality of manners, such as a fastener connection manner, a snap connection manner, a thread connection manner, and the like, and is not limited herein.
Further, referring to fig. 2 and 4, a sealing member 191 is disposed on the bottom cover, and the sealing member 191 is tightly pressed between the bottom cover 19 and the outer edge of the opening. The sealing performance of the liquid storage tank 10 can be enhanced by arranging the sealing member 191, and the heat exchange liquid is prevented from leaking.
Specifically, the sealing member 191 may be a sealing ring disposed along an outer edge of the opening. Of course, other forms of seals 191, such as gasket seals, may be used.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A constant-temperature perfusion type isolated organ perfusion container is characterized by comprising:
the liquid storage tank is internally divided into a first cavity and a second cavity, the first cavity is used for containing perfusate, the second cavity is used for containing heat exchange liquid, the heat of the heat exchange liquid can be transferred from the second cavity to the perfusate in the first cavity, the liquid storage tank is provided with a mounting hole, and the mounting hole is positioned above the first cavity; the liquid storage tank is provided with a liquid adding port communicated with the first cavity, and the liquid storage tank is also provided with a liquid discharging port communicated with the first cavity;
support the membrane, it installs to support the membrane cover on the mounting hole and be located the top of first cavity, support the membrane and be used for placing the isolated organ, still be equipped with on the liquid storage pot and be used for with the liquid outlet of first cavity and isolated organ pipeline intercommunication, support be equipped with on the membrane be used for with the small opening of first cavity intercommunication, perfusate in the first cavity can be followed the liquid outlet flows in the isolated organ along the pipeline, follows again after flowing in the isolated organ the small opening on the support membrane flows in the first cavity.
2. The perfusion vessel according to claim 1, wherein the second cavity is circumferentially disposed around the outer circumferential wall of the first cavity, or the first cavity is circumferentially disposed around the outer circumferential wall of the second cavity.
3. The perfusion vessel of claim 1, wherein the support membrane is concave towards the first cavity.
4. The perfusion vessel according to claim 1, wherein the support membrane is a soft medical membrane.
5. The perfusion vessel of claim 1, wherein the reservoir is a transparent tank, and a silk screen is disposed on a sidewall of the reservoir to indicate the volume of the perfusate.
6. The perfusion vessel of claim 1, wherein the reservoir is provided with a heat exchange inlet and a heat exchange outlet for communicating with the second chamber, respectively, and the heat exchange inlet and the heat exchange outlet are connected to a heating device pipeline to form a heat exchange circulation path.
7. The perfusion vessel of claim 6, wherein a temperature measuring port is disposed on the reservoir, and a temperature detecting unit for detecting the temperature of the perfusate or the heat exchange fluid is disposed in the temperature measuring port.
8. The perfusion vessel according to claim 6, wherein the heat exchange outlet is disposed at a lower portion of the second chamber, and the heat exchange inlet is disposed at an upper portion of the second chamber.
9. The perfusion vessel according to any one of claims 1-8, wherein the bottom of the fluid reservoir is provided with an opening for communicating the second chamber with the outside, and the fluid reservoir is detachably provided with a bottom cover for covering the opening.
10. The perfusion vessel of claim 9, wherein the bottom cover has a sealing member, the sealing member being compressed between the bottom cover and the outer edge of the opening.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910284008.8A CN109907037B (en) | 2019-04-10 | 2019-04-10 | Constant temperature perfusion type isolated organ perfusion container |
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| CN201910284008.8A CN109907037B (en) | 2019-04-10 | 2019-04-10 | Constant temperature perfusion type isolated organ perfusion container |
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| CN109907037B true CN109907037B (en) | 2022-02-01 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2021036742A1 (en) * | 2019-08-26 | 2021-03-04 | 无锡市人民医院 | Ex vivo organ perfusion apparatus |
| CN110506734A (en) * | 2019-08-26 | 2019-11-29 | 陈静瑜 | Isolated organ perfusion equipment |
| WO2021036738A1 (en) * | 2019-08-26 | 2021-03-04 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Ex vivo organ perfusion container |
| CN110506733A (en) * | 2019-08-26 | 2019-11-29 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Isolated organ perfusion container |
| CN111903664A (en) * | 2020-08-17 | 2020-11-10 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Isolated organ perfusion system and liver storage device |
| CN115777688B (en) * | 2022-11-03 | 2023-05-12 | 河南中医药大学第一附属医院 | Isolated myocardial tissue perfusion experiment system |
| CN117796389B (en) * | 2024-03-01 | 2024-06-11 | 青岛市中医医院(青岛市海慈医院、青岛市康复医学研究所) | Human organ tissue preservation equipment for urology department |
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