CN111903664A

CN111903664A - Isolated organ perfusion system and liver storage device

Info

Publication number: CN111903664A
Application number: CN202010823527.XA
Authority: CN
Inventors: 吴懿苗; 梁铭炬; 许晓珊; 周伟津; 陈素平; 郭家钘
Original assignee: Guangdong Shunde Industrial Design Institute
Current assignee: Guangdong Shunde Industrial Design Institute
Priority date: 2020-08-17
Filing date: 2020-08-17
Publication date: 2020-11-10

Abstract

The invention relates to an isolated organ perfusion system and a liver storage device. The container body is used for containing perfusate. The breathing air bag is arranged in the container body and used for supporting the isolated organ. Foretell liver storage device, through the inside of storing up the liver ware add establish the breathing airbag, place the isolated organ on the breathing airbag, one of simulation isolated organ breathes one during the perfusion, bleed and breathe in for the breathing airbag, make the breathing airbag continuously relax at the perfusion in-process, the isolated organ is corresponding changing with the point of breathing airbag contact surface, can make isolated organ more smooth and easy at the perfusion in-process blood circulation like this, avoid isolated organ surface because of receiving fixed pressure for a long time and produce the thrombus, can effectively eliminate invalid perfusion area, reduce continuous local compression necrosis.

Description

Isolated organ perfusion system and liver storage device

Technical Field

The invention relates to the technical field of perfusion, in particular to an isolated organ perfusion system and a liver storage device.

Background

Liver transplantation is the only effective means for treating end-stage liver diseases, and because organs are in short supply, a plurality of end-stage liver disease patients and the like die due to not being transplanted with proper liver sources, marginal liver supply transplantation has great significance for the field of liver transplantation. The peripheral liver supply has poor quality, the damage of the peripheral liver supply can be further aggravated by the traditional static cold storage, and the quality of the peripheral liver supply can be effectively improved by the Mechanical Perfusion (MP) technology of an isolated organ, so that the number of transplantable livers is increased.

The isolated organ mechanical perfusion is a mode for preserving and transferring organs, a liver is obtained and then a blood vessel of the liver is connected to an isolated organ mechanical perfusion system, and the isolated organ mechanical perfusion system continuously perfuses perfusion liquid to the liver in the liver preservation and transfer stage and simultaneously supplies oxygen, nutrients and the like to the liver. The liver storage device is the core part of the isolated organ mechanical perfusion system, and the liver is placed in the liver storage device during perfusion and is connected to a circulation pipeline through a cannula and a connector on the liver storage device. The perfusion mode of the liver in the mechanical perfusion system can be divided into two modes according to the pipeline connection mode: firstly, closed-loop perfusion is carried out, namely a consumable pipeline is connected with hepatic artery and portal vein of the liver through an intubation tube and is used as a perfusion liquid to enter a liquid inlet of the liver; the lower vena cava of the liver is connected to the consumable pipeline through the cannula, and the lower vena cava is used as a liquid outlet of the liver as perfusate to form a closed circulating pipeline. Secondly, open-loop perfusion, namely a consumable pipeline is connected with hepatic artery and portal vein of the liver through an intubation tube and used as a perfusion liquid to enter a liquid inlet of the liver; the inferior vena cava of the liver is not connected with any cannula, perfusate directly flows out of the liver storage device from the inferior vena cava after flowing through the liver, and is discharged into a consumable pipeline from a liquid outlet of the liver storage device to form an open-loop circulation pipeline.

During perfusion, the liver storage device is used as an intermediate carrier to connect the liver to the circulating pipeline for circulating perfusion, so that the design of the liver storage device meets the requirements that the liver is comfortably perfused and can be perfectly connected to the circulating pipeline for perfusion. However, in the perfusion process, the isolated organ is supported by the bottom of the liver storage device, the supporting surface is in contact with the liver surface, and the surface of the liver in contact with the supporting surface is subjected to fixed pressure for a long time, so that the unsmooth blood circulation in the surface of the isolated organ in contact with the supporting surface is easily caused in the perfusion process, and thrombus is caused.

Disclosure of Invention

Therefore, it is necessary to overcome the defects of the prior art and provide an isolated organ perfusion system and a liver storage device, which can minimize the generation of thrombus in the isolated organ during perfusion.

The technical scheme is as follows: a liver reservoir, the liver reservoir comprising: the container body is used for containing perfusate; and the breathing air bag is arranged in the container main body and is used for supporting the isolated organ.

Foretell liver storage device, add through the inside of storing up the liver ware and establish the breathing airbag, place the isolated organ on the breathing airbag, imitate the oppression of diaphragm to isolated organ during the perfusion, bleed and breathe in for the breathing airbag, make the breathing airbag continuously relax at the perfusion in-process, isolated organ is corresponding in the change with the point of breathing airbag contact surface, can make isolated organ more smooth and easy at perfusion in-process blood circulation like this, avoid isolated organ surface because of receiving fixed pressure for a long time and produce the thrombus, effectively eliminate invalid perfusion area, reduce continuous local compression necrosis.

In one embodiment, the central portion of the respiration air bag is provided with at least one aperture.

In one embodiment, the breathing air bag comprises an annular bag body, a hollow main prism body and a plurality of hollow branch bodies; the annular bag body is arranged around the peripheries of the main prism body and the plurality of branch bodies, two ends of the main prism body are respectively communicated with the annular bag body, the main prism body is communicated with the branch bodies, the branch bodies are arranged at intervals along the main prism body in sequence, and the adjacent branch bodies, the main prism body and the annular bag body are surrounded to form the hole.

In one embodiment, the breathing air bag is provided with an air pipe, and the air pipe is used for being connected with an external control air source.

In one embodiment, the container main body comprises a bottom plate and a side plate connected with the bottom plate, the side plate is arranged around the bottom plate, a concave surface plate is arranged in the middle of the bottom plate, a liquid outlet is formed in the concave surface plate, and the breathing air bag is arranged in the concave surface plate.

In one embodiment, a liquid guide groove is formed in the bottom portion of the concave panel, the liquid outlet is arranged on the bottom wall of the liquid guide groove, the bottom wall of the liquid guide groove is an inclined wall which is obliquely arranged, the liquid outlet is arranged at the lower portion of the inclined wall, and a liquid outlet joint is arranged at the liquid outlet.

In one embodiment, the concave panel is a semi-ellipsoidal panel or a semi-hemispherical panel.

In one embodiment, the breathing air bag is in an elliptical ring shape, and the size of the outermost elliptic curve of the breathing air bag is matched with the size of the section elliptic curve of the elliptic curved surface of the concave panel.

In one embodiment, the liver storage device further comprises a moisture retention membrane and a cover body, wherein the moisture retention membrane is arranged inside the container body and is used for being arranged above the isolated organ; the lid is provided to the mouth of the container body so as to be openable.

The utility model provides an isolated organ perfusion system, includes the liver storage ware, still includes the consumptive material pipeline, the liquid outlet of liver storage ware with the feed liquor end of consumptive material pipeline is linked together, the play liquid end of consumptive material pipeline is used for with be located the inside isolated organ of liver storage ware is linked together.

Foretell isolated organ perfusion system, establish the breathing airbag through the inside of storing up the liver ware with adding, place the isolated organ on the breathing airbag, imitate the oppression of diaphragm to the isolated organ during the perfusion, bleed and breathe in for the breathing airbag, make the breathing airbag continuously relax at the perfusion in-process, the isolated organ is corresponding in the change with the point of breathing airbag contact surface, it is more smooth and easy to make isolated organ blood flow at the perfusion in-process like this, avoid isolated organ surface because of receiving fixed pressure for a long time and produce the thrombus, effectively eliminate invalid perfusion area, reduce continuous local compression necrosis.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention.

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

FIG. 1 is a schematic structural view of a liver storage device with a breathing air bag inside according to an embodiment of the present invention;

FIG. 2 is a perspective view of a respiratory bladder according to an embodiment of the present invention;

FIG. 3 is a view of another perspective of a resuscitation bag according to an embodiment of the present invention;

FIG. 4 is a schematic view of a perspective view of an isolated organ positioned over the balloon of FIG. 1;

FIG. 5 is a schematic view of another perspective of the placement of an isolated organ over the balloon of FIG. 1;

FIG. 6 is a schematic view of a container body according to an embodiment of the present invention from one perspective;

FIG. 7 is a schematic structural view of another perspective of a container body according to an embodiment of the present invention;

FIG. 8 is a schematic structural view of a container body from yet another perspective in accordance with an embodiment of the present invention;

FIG. 9 is a view showing a structure of one of the viewing angles of a moisture-retaining film provided in a container main body according to an embodiment of the present invention;

FIG. 10 is a schematic view of a container body with a cover according to an embodiment of the present invention;

FIG. 11 is a block diagram of a junction block assembly in combination with an organ ex vivo in accordance with an embodiment of the present invention;

FIG. 12 is a schematic structural view of a junction block assembly according to an embodiment of the present invention;

FIG. 13 is a schematic structural view of a junction block assembly according to an embodiment of the present invention;

fig. 14 is a schematic structural diagram of an ex vivo organ perfusion system according to an embodiment of the present invention.

10. A liver storage device; 11. a container body; 111. a base plate; 1111. a concave panel; 1112. a liquid outlet; 1113. a liquid guide groove; 1114. a liquid outlet joint; 1115. reinforcing rib plates; 112. a side plate; 1121. a front side plate; 1122. a rear side plate; 1123. a left side plate; 1124. a right side plate; 12. a breathing air bag; 121. a pore; 122. a toroidal bladder; 123. a main prism; 124. a branching body; 125. an air tube; 13. a moisture retention film; 14. a cover body; 15. a fastener; 161. 162, 163, 164, 165, bulkhead fitting; 17. a back-off structure; 20. an isolated organ; 21. an artery;

30. a junction block assembly; 31. a cannula holder; 311. a through hole; 312. a first compression end face; 313. a screw hole; 32. a docking station; 321. a second compression end face; 322. a connecting pipe; 323. mounting holes; 324. a mounting member; 325. a side hole luer fitting;

40. a power pump; 50. a membrane lung; 61. an air-oxygen mixer; 62. an oxygen tank; 63. a carbon dioxide tank; 64. a heat exchange device; 71. a microembolus filter; 72. a pressure sensor; 73. a flow sensor; 74. a host; 75. a display screen; 76. a power source; 77. a bile accommodation apparatus.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Referring to fig. 1 to 3 and 1 to 3, fig. 1 is a schematic structural view illustrating a breathing bag 12 placed inside a liver storage device 10 according to an embodiment of the present invention, fig. 2 is a structural view illustrating the breathing bag 12 according to an embodiment of the present invention, and fig. 3 is a structural view illustrating another perspective view illustrating the breathing bag 12 according to an embodiment of the present invention. According to an embodiment of the present invention, a liver storage device 10 includes a container body 11 and a breathing airbag 12. The container body 11 is used for containing a perfusate. A respiration bag 12 is provided inside the container body 11, and the respiration bag 12 supports an isolated organ 20.

Referring to fig. 1, 4 and 5, fig. 4 is a schematic view of an isolated organ 20 placed above the balloon of fig. 1, and fig. 5 is a schematic view of an isolated organ 20 placed above the balloon of fig. 1. The liver storage device 10 is characterized in that the breathing air bag 12 is additionally arranged in the liver storage device 10, the isolated organ 20 is placed on the breathing air bag 12, the compression of the isolated organ 20 by diaphragm muscles is simulated during perfusion, air suction and air suction are performed on the breathing air bag 12, the breathing air bag 12 continuously relaxes in the perfusion process, the point of the contact surface of the isolated organ 20 and the breathing air bag 12 correspondingly changes, so that the blood circulation of the isolated organ 20 in the perfusion process is smoother, thrombus generated on the surface of the isolated organ 20 due to long-time fixed pressure is avoided, ineffective perfusion areas can be effectively eliminated, and continuous local compression necrosis is reduced.

Referring to fig. 1 to 3, further, at least one aperture 121 is formed in the middle portion of the breathing bag 12. In this way, the perfusion fluid discharged from the isolated organ 20 placed on the breathing bag 12 during perfusion flows to the bottom of the container body 11 through the aperture 121, and is delivered to the consumable line through the liquid outlet 1112 at the bottom of the container body 11.

Referring to fig. 1 to 3, further, the airbag 12 includes a ring-shaped bag body 122, a hollow main rib 123 and a plurality of hollow branches 124. The annular bag body 122 is arranged around the periphery of the main prism body 123 and the plurality of branch bodies 124, two ends of the main prism body 123 are respectively communicated with the annular bag body 122, the main prism body 123 is communicated with the branch bodies 124, the branch bodies 124 are sequentially arranged along the main prism body 123 at intervals, and the adjacent branch bodies 124, the main prism body 123 and the annular bag body 122 are surrounded to form a pore 121. Therefore, on one hand, one side surface of the respiration air bag 12 formed by combining the annular bag body 122, the main prism body 123 and the branch body 124 is not a plane but a concave-convex surface, so that the isolated organ 20 has a certain fixing effect and the isolated organ 20 is prevented from greatly moving on the supporting surface of the respiration air bag 12; on the other hand, it can be realized that a plurality of pores 121 are formed on the respiration air bag 12, and the perfusate enters the bottom of the liver storage device 10 through the pores 121.

Referring to fig. 1 to 3, further, the breathing air bag 12 is provided with an air tube 125, and the air tube 125 is used for connecting with an external control air source. Thus, the external control air source can perform air suction and air suction to the breathing air bag 12 through the air tube 125, so that the breathing air bag 12 can continuously relax during the perfusion process.

Referring to fig. 1, 6 to 8, fig. 6 illustrates a structural schematic diagram of a container main body 11 according to an embodiment of the present invention from one viewing angle, fig. 7 illustrates a structural schematic diagram of a container main body 11 according to an embodiment of the present invention from another viewing angle, and fig. 8 illustrates a structural schematic diagram of a container main body 11 according to an embodiment of the present invention from another viewing angle. Further, the container body 11 includes a bottom plate 111 and a side plate 112 connected to the bottom plate 111. The side plate 112 is arranged around the bottom plate 111, a concave surface plate 1111 is arranged at the middle part of the bottom plate 111, a liquid outlet 1112 is arranged on the concave surface plate 1111, and the breathing air bag 12 is arranged in the concave surface plate 1111.

Referring to fig. 6 and 7, a liquid guiding groove 1113 is disposed at a bottom portion of the concave plate 1111, a liquid outlet 1112 is disposed on a bottom wall of the liquid guiding groove 1113, the bottom wall of the liquid guiding groove 1113 is an inclined wall disposed obliquely, the liquid outlet 1112 is disposed at a lower portion of the inclined wall, and a liquid outlet joint 1114 is disposed at the liquid outlet 1112. Specifically, the number of the liquid outlets 1112 is two, the number of the liquid outlet joints 1114 is two, the two liquid outlet joints 1114 are disposed in the two liquid outlets 1112 in a one-to-one correspondence manner, one of the liquid outlet joints 1114 guides the perfusion fluid into the consumable line corresponding to the artery 21, and the other liquid outlet joint 1114 guides the perfusion fluid into the consumable line corresponding to the portal vein.

Thus, all the perfusion liquid can participate in the blood circulation in the perfusion process, and no effusion exists in the container body 11 in each circulation. The liquid guiding groove 1113 is designed to more effectively guide the discharge of the perfusion liquid in the perfusion process, so as to avoid the formation of the accumulated liquid into thrombus, and the liquid outlet joint 1114 is arranged at the lowest point of the low liquid guiding groove 1113, so that the perfusion liquid can rapidly flow out of the container body 11 and carry out the next blood circulation. Since the perfusion mode is an open-loop perfusion mode, i.e. the perfusate passing through the isolated organ 20 is directly discharged into the liver storage device 10, the liquid is led out from the liquid outlet 1112 of the liver storage device 10 for the next perfusion cycle. In order to avoid the occurrence of evacuation (evacuation condition is that the flow of the liquid flowing out of the isolated organ 20 at the same time is smaller than the flow flowing out of the liver storage device 10), the perfusate needs to store a certain amount of stable perfusate at the bottom of the liver storage device 10 to cover the liquid outlet 1112 for a long time, so that the perfusate can be timely replenished when the outflow perfusate of the liver storage container and the liver is poor.

Referring to fig. 6 and 7, further, the concave plate 1111 is a semi-elliptic plate or a semi-spherical plate. The concave plate 1111 is not limited to the semi-elliptic spherical plate or the semi-spherical plate in the present embodiment, and may have other shapes, which are not limited herein. Thus, the design scheme of the bottom of the semi-elliptic spherical plate or the semi-spherical plate has the advantages that the horizontal cross section area on the vertical height is gradually reduced from top to bottom, so that the volume of the lowest part is minimum, and compared with the design of equal cross section area on the vertical height, the perfusion liquid is saved. That is, can avoid the corner position of storing up liver ware 10 to have more hydrops, avoid the great storage liver ware 10 of bottom area to consume more perfusate at the filling in-process, can realize that the perfusate in the storage liver ware 10 assembles and concentrates in leading the cistern 1113 to outwards discharge in the consumptive material pipeline through going out liquid joint 1114. In addition, when the concave plate 1111 is a semi-elliptic spherical plate or a semi-spherical plate, the perfusion fluid can be better converged and concentrated into the bottom of the liquid guide groove 1113.

Referring to fig. 6 and 7, further, reinforcing rib plates 1115 are disposed on the bottom surface of the bottom plate 111, the reinforcing rib plates 1115 are divided into reinforcing rib plates 1115 disposed horizontally and reinforcing rib plates 1115 disposed longitudinally, and the reinforcing rib plates 1115 correspond to the base of the liver storage device 10.

Referring to fig. 1 to 3, in one embodiment, the respiration air bag 12 is in an elliptical ring shape, and the outermost elliptical curve of the respiration air bag 12 matches the cross-sectional elliptical curve of the concave plate 1111. Therefore, the breathing air bag 12 can be just placed in the concave panel 1111, and the fixing effect of the breathing air bag 12 in the concave panel 1111 is better.

Referring to fig. 9 and 10, fig. 9 is a schematic view of a structure of a moisture retention film 13 disposed in a container body 11 according to an embodiment of the present invention, and fig. 10 is a schematic view of a structure of a cover 14 disposed on the container body 11 according to an embodiment of the present invention. In one embodiment, the liver storage device 10 further comprises a moisture retention membrane 13 and a cover 14. The moisture retention film 13 is provided inside the container body 11 and is provided above the isolated organ 20. In this way, the isolated organ 20 is covered above the isolated organ 20 during the mechanical perfusion process, and the water loss on the surface of the portal vein, the artery 21 and the liver during the perfusion process is prevented, so that the epidermal cells are damaged. In addition, the moisturizing membrane 13 is for example fixed with the back-off structure 17 that sets up on the liver storage device 10 and is fixed on the liver storage device 10 to can make the isolated organ 20 have certain spacing on the vertical face, avoid isolated organ 20 to upwards remove.

Referring to fig. 10, a cover 14 is detachably disposed at the mouth of the container body 11. When it is desired to access the isolated organ 20, the cover 14 is opened. In the process of placing the isolated organ 20 into the liver storage device 10 for perfusion, the cover body 14 is closed, and the liver storage device 10 is sealed, so that the protection and moisture preservation effects are achieved. The cover 14 is detachably connected to the mouth of the liver storage device 10 by a plurality of fasteners 15, for example.

Referring to fig. 14, fig. 14 is a schematic structural diagram of an isolated organ 20 perfusion system according to an embodiment of the present invention. In one embodiment, an isolated organ 20 perfusion system comprises the liver storage device 10 according to any of the above embodiments, and further comprises a consumable pipeline, wherein the liquid outlet 1112 of the liver storage device 10 is communicated with the liquid inlet end of the consumable pipeline, and the liquid outlet end of the consumable pipeline is used for being communicated with the isolated organ 20 inside the liver storage device 10.

The isolated organ 20 perfusion system is characterized in that the breathing air bag 12 is additionally arranged in the liver storage device 10, the isolated organ 20 is placed on the breathing air bag 12, the compression of diaphragm on the isolated organ 20 is simulated during perfusion, air suction and air suction are performed on the breathing air bag 12, the breathing air bag 12 continuously relaxes in the perfusion process, the point of the contact surface of the isolated organ 20 and the breathing air bag 12 correspondingly changes, so that the blood circulation of the isolated organ 20 in the perfusion process is smoother, thrombus caused by long-time fixed pressure on the surface of the isolated organ 20 is avoided, an invalid perfusion area can be effectively eliminated, and continuous local compression necrosis is reduced.

Generally, the insertion tube corresponding to the conventional hepatic artery 21 and the insertion tube corresponding to the portal vein are generally in a tube shape, after the insertion tubes are inserted into the hepatic artery 21 or the hepatic portal vein, medical staff still need to tie and fix the insertion tubes by using an operation line, the operation is complex, the surgical line is not tied firmly, the risk of dropping the insertion tubes in the perfusion process exists, and the endothelial tissue of the portal vein or the inherent hepatic artery 21 can be damaged in the process of inserting the insertion tubes into the hepatic artery 21 or the portal vein.

Referring to fig. 11 to 13, fig. 11 is a schematic diagram illustrating a joint tube assembly 30 according to an embodiment of the present invention combined with an isolated organ 20, fig. 12 is a schematic diagram illustrating a joint tube assembly 30 according to an embodiment of the present invention, and fig. 13 is a schematic diagram illustrating a joint tube assembly 30 according to an embodiment of the present invention. Based on this, the isolated organ 20 perfusion system further includes a connector tube assembly 30 disposed between the isolated organ 20 and the consumable pipeline, and the artery 21 or portal vein of the isolated organ 20 is connected to the outlet end of the consumable pipeline through the connector tube assembly 30. The joint pipe assembly 30 includes: a socket 31 and a docking socket 32. The cannula holder 31 is provided with a through hole 311 for inserting the artery 21 or the portal vein of the isolated organ 20, the cannula holder 31 is provided with a first pressing end surface 312, and the through hole 311 is located in the middle of the first pressing end surface 312. The docking seat 32 is provided with a docking hole, and the docking hole is arranged corresponding to the through hole 311. The docking cradle 32 is also provided with a second compression end face 321. The abutting hole is located in the middle of the second pressing end face 321, and the second pressing end face 321 is in press fit with the first pressing end face 312. The docking seat 32 is further provided with a connecting tube 322 communicated with the docking hole, and the connecting tube 322 is used for being communicated with the consumable pipeline. In this way, the artery 21 or portal vein of the isolated organ 20 can be connected to the consumable line, so that the artery 21 or portal vein of the isolated organ 20 is accessed into the perfusion circuit. Taking the artery 21 of the isolated organ 20 and the consumable pipeline to be connected as an example, the connection process of the artery 21 and the connector tube assembly 30 is described, the trunk at the end of the artery 21 is reserved before perfusion, and the trunk at the end of the artery 21 is cut into sheets circumferentially arranged around the end of the artery 21, the artery 21 is inserted into the through hole 311, so that the sheets at the end of the artery 21 are flatly laid on the first pressing end surface 312, the first pressing end surface 312 of the butt joint seat 32 is in press fit with the second pressing end surface 321 of the cannula seat 31, at this time, the butt joint hole is communicated with the artery 21, in addition, the connecting pipe 322 is communicated with the consumable pipeline, and the artery 21 of the isolated organ 20 is communicated with the consumable pipeline. The isolated organ 20 can start perfusion after being connected into the perfusion circulation pipeline, and after the perfusion is finished, the butt joint seat 32 and the cannula seat 31 are separated, and the sheet material at the tail end of the artery 21 is trimmed off, so that the perfusion is finished. It can be seen that, instead of inserting the cannula directly into the artery 21 as in the conventional method, the cannula does not contact the inner wall of the artery 21 during the perfusion process, so that the injury to the endothelial tissue of the artery 21 is avoided, and meanwhile, the suture operation is not required, so that the working efficiency of assembly is greatly improved, and in addition, the firmness of the combination of the connector tube assembly 30 and the artery 21 or the portal vein of the isolated organ 20 is higher, so that the risk of falling off during the perfusion process is avoided.

Referring to fig. 11 to 13, further, one side of the cannula holder 31 is rotatably connected to one side of the docking holder 32, and the other side of the cannula holder 31 is detachably connected to the other side of the docking holder 32 through the mounting member 324. Thus, the opening and closing operation between the socket 31 and the docking cradle 32 can be facilitated.

Referring to fig. 11 to 13, further, a screw hole 313 is disposed on a side portion of the cannula holder 31, a mounting hole 323 corresponding to the screw hole 313 is disposed on a side portion of the docking holder 32, and the mounting member 324 is a screw rod corresponding to the screw hole 313.

In an alternative embodiment, instead of a combination of rotatably connecting one side of the cannula holder 31 and the docking holder 32 and detachably connecting the other side of the cannula holder 31 and the docking holder 32, the cannula holder 31 is integrally detachably connected to the docking holder 32, and specifically, the connection between the cannula holder 31 and the docking holder 32 may be, for example, a snap connection, a rivet connection, a screw connection, a bolt connection, a pin connection, and the like, which is not limited herein.

Referring to fig. 11 to 13, in one embodiment, the first pressing end surface 312 is a plane, and the second pressing end surface 321 is a plane. Thus, when the first compressing end surface 312 and the second compressing end surface 321 are in compression fit, the sheet at the end of the artery 21 can be firmly compressed, and the sealing performance is ensured. Alternatively, the first pressing end surface 312 may also be an arc-shaped surface, and the second pressing end surface 321 is an arc-shaped surface corresponding to the first pressing end surface 312. The first pressing end surface 312 may be a surface having another shape, which is not limited herein, as long as the second pressing end surface 321 is adapted to the shape of the first pressing end surface 312, so that the first pressing end surface 312 and the second pressing end surface 321 can be tightly fitted to each other to firmly press the sheet material at the end of the artery 21.

Referring to fig. 11-13, in one embodiment, the connector tube assembly 30 further includes a cannula connector (specifically, two plate connectors 161 shown in fig. 10) disposed between the connection tube 322 and the consumable tubing. The intubation joint 161 is used to be installed on the wall of the liver storage device 10, and the connection tube 322 is connected with the consumable pipeline through the intubation joint 161. Thus, it is convenient to connect the connection tube 322 inside the liver storage 30 to the consumable line outside the liver storage 30.

Referring to fig. 11-13, in one embodiment, a lateral port luer 325 is provided on docking cradle 32. A lateral bore luer fitting 325 is located on the sidewall of the connecting tube 322 and communicates with the connecting tube 322. Therefore, the pressure pipe can be directly connected to the lateral hole luer connector 325, the position of the pressure connector is arranged on the butt-joint seat 32, the distance between the pressure connector and the portal vein or artery 21 is very close, the pressure of the portal vein or artery 21 can be measured more truly, and the situation that the pressure pipe is connected through installing the connector in the middle of the circulating pipeline and is far away from the portal vein or artery 21 to generate pressure difference to cause large measurement errors is avoided.

Referring to fig. 4 and 14, further, the connector tube assemblies 30 are two, one connector tube assembly 30 is used for communicating with the artery 21 of the isolated organ 20, the other connector tube assembly 30 is used for communicating with the portal vein of the isolated organ 20, and the connection tube 322 is communicated with the consumable pipeline.

Furthermore, it is understood that there are two consumable lines, which are arranged in one-to-one correspondence with the artery 21 and the portal vein of the isolated organ 20. One of the consumable pipelines, the artery 21 of the isolated organ 20 and the liver storage device 10 form a perfusion circulation loop, perfusate flows out of the consumable pipeline through the liver storage device 10, circularly flows into the artery 21 of the isolated organ 20 through the consumable pipeline, is discharged into the liver storage device 10 through the isolated organ 20, and circulates in the way. Another consumptive material pipeline, the portal vein of isolated organ 20 and liver storage device 10 form the circulation return circuit of filling, and the perfusate flows out to the consumptive material pipeline through liver storage device 10 to in the portal vein of isolated organ 20 is flowed in to the circulation of consumptive material pipeline, is discharged to liver storage device 10 by isolated organ 20, so circulation.

Referring to fig. 14, further, the consumable pipeline is provided with a power pump 40 and a membrane 50. Under the action of the power pump 40, the perfusate in the liver storage device 10 enters the membrane lung 50, passes through the membrane lung 50 and then enters the artery 21 or the portal vein of the isolated organ 20.

Referring to fig. 14, in one embodiment, the membrane 50 is provided with a mixed gas port for communicating with a mixed gas source line. The perfusate combines with the gas mixture in the membrane lung 50 to form an oxygenated perfusate to ensure that the perfusate flowing into the artery 21 has sufficient oxygen content. Specifically, the air-oxygen mixer 61 supplies a mixed gas source, the air-oxygen mixer 61 is respectively communicated with an oxygen tank 62 and a carbon dioxide tank 63 through pipelines, and the air-oxygen mixer 61 is communicated with a mixed gas interface. The oxygen from the oxygen tank 62 and the carbon dioxide from the carbon dioxide tube are mixed in the air-oxygen mixer 61 to obtain a mixed gas source, and the mixed gas source is sent into the membrane lung 50 through the mixed gas interface.

Referring to fig. 14, in addition, the membrane lung 50 is communicated with the heat exchange device 64 through a heat exchange tube to form a circulating heat exchange loop, and the perfusate exchanges heat with a heat exchange medium of the circulating heat exchange loop in the membrane lung 50 to keep the temperature of the perfusate suitable for the isolated organ 20. The heat exchanging device 64 is, for example, a constant temperature water tank, so that the temperature of the perfusion fluid is maintained at a predetermined temperature, for example, 37 ℃.

Referring to fig. 14, a microembolus filter 71 is further disposed on the consumable pipeline, and the microembolus filter 71 may be disposed on a pipeline connected to the liquid inlet end of the membrane lung 50, or a pipeline connected to the liquid outlet end of the membrane lung 50. Thus, the micro-suppository filter 71 is used for filtering various micro-suppositories in the perfusate, preventing the micro-blood vessels of the isolated organ 20 from being embolized due to various micro-suppositories such as thrombus or air embolism, effectively improving the blood perfusion of the micro-blood vessels of the human body, and further improving the utilization rate of the donor organ and the success rate of the transplantation operation.

Referring to fig. 14, a pressure sensor 72 is further disposed on the consumable pipeline. The pressure sensor 72 is disposed at the fluid outlet end of the membranous lung 50 and is located close to the portal or artery 21 to measure the pressure in the portal or artery 21. Specifically, referring to fig. 11-13, a side hole luer 325 is provided on the docking station 32. A lateral bore luer fitting 325 is located on the sidewall of the connecting tube 322 and communicates with the connecting tube 322. Therefore, the pressure sensor 72 is directly connected to the lateral hole luer connector 325 through a pressure pipe, the position of the pressure connector is arranged on the docking seat 32, and the distance between the pressure connector and the portal vein or artery 21 is very close, so that the pressure of the portal vein or artery 21 can be measured more truly, and the situation that the pressure pipe is connected through a connector arranged in the middle of a circulating pipeline and is far away from the portal vein or artery 21, pressure difference is generated, and large measurement errors are caused is avoided.

Referring to fig. 14, a flow sensor 73 is further disposed on the consumable pipeline. The flow sensor 73 is disposed on the pipeline between the power pump 40 and the micro-suppository filter 71 or at the outlet end of the membrane lung 50, and the specific location is not limited herein. The flow sensor 73 can acquire the flow of the perfusion fluid on the consumable pipeline, and can judge whether the power pump 40, the micro-suppository filter 71 and the membrane lung 50 work normally or not according to the flow.

Referring to fig. 14, further, the isolated organ 20 perfusion system further includes a host 74, a display 75 and a power supply 76. The power supply 76 is electrically connected with the host 74, the display screen 75, the power pump 40 and the heat exchange device 64, the host 74 is electrically connected with the display screen 75, the heat exchange device 64, the pressure sensor 72 and the flow sensor 73, the host 74 can correspondingly control the display screen 75, the heat exchange device 64, the pressure sensor 72 and the flow sensor 73 to work, and the display screen 75 is used for displaying the detection data of the pressure sensor 72 and the flow sensor 73.

Referring to fig. 4 and 8, in one embodiment, the side plate 112 is a stepped plate, and the size of the opening of the side plate 112 decreases from the top to the bottom. Thus, the top opening of the side plate 112 is larger than the bottom opening of the side plate 112, and the side plate 112 is stepped, so that the top of the liver storage device 10 has enough space for the connection joint, the joint pipe assembly 30 and the related pipeline.

Referring to fig. 4 and 8, a plurality of plate penetrating connectors (161, 162, 163, 164, 165) are further disposed on the side plate 112. The plate penetrating joints (161, 162, 163, 164 and 165) can realize the corresponding connection of the connecting pipe 322, the side hole luer joint 325 and the breathing air bag 12 in the liver storage device 10 and the external structure. Specifically, two of the plate penetrating connectors 161 are cannula connectors, the connection tube 322 is correspondingly connected to the cannula connector 161 through a hose, and the cannula connector 161 is connected to the consumable pipeline through a pipeline (e.g., a hose). Two of the two through-plate connectors 342 are pressure tube connectors, the lateral port luer 325 is connected to a pressure tube connector via tubing (e.g., a hose), the pressure tube connector is connected to the pressure sensor 72 via tubing (e.g., a hose), and the pressure sensor 72 senses the pressure level at the lateral port luer 325 accordingly. The two plate connectors 163 are air tubes 125 connectors, the air inlets and the air outlets of the breathing air bags 12 are respectively connected with the two air tubes 125 connectors, and the two air tubes 125 connectors are connected with external air transmission equipment. In addition, one of the penetrating plate joints 164 is a bile pipe joint, the bile duct of the isolated organ 20 is connected to the bile pipe joint through a pipeline (e.g., a flexible pipe), and the bile duct of the isolated organ 20 discharges the bile to the outside through the flexible pipe and the bile pipe joint to a bile accommodating device 77 (shown in fig. 14). The excess bulkhead connectors 165 are spare connectors.

Referring to fig. 4 and 8, the side panel 112 further includes a front side panel 1121, a rear side panel 1122, a left side panel 1123, and a right side panel 1124. The front side plate 1121 and the rear side plate 1122 are disposed opposite to each other, the left side plate 1123 and the right side plate 1124 are disposed opposite to each other, and the front side plate 1121, the rear side plate 1122, the left side plate 1123 and the right side plate 1124 are respectively surrounded around the bottom plate 111. The front side plate 1121 is stepped, joint hole positions are respectively arranged on the vertical surfaces of the upper step and the lower step of the front side plate 1121, and the plate penetrating joints are arranged in the joint hole positions. Two joint hole locations are also provided on the left side plate 1123.

Referring to fig. 4, 8 and 9, further, for example, four of the inverted structures 17 are provided, two of which are disposed on the front side plate 1121, and the other two of which are disposed on the bottom plate 111 and are close to the rear side plate 1122. Thus, one side of the moisture retention membrane 13 is connected with the two inverted structures 17 on the front side plate 1121, the other side of the moisture retention membrane 13 is connected with the two inverted structures 17 on the bottom plate 111, and the fixing position of one side of the moisture retention membrane 13 is higher than the fixing position of the other side of the moisture retention membrane 13, so that a better fixing effect on the isolated organ 20 can be realized.

The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above examples only show some embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Claims

1. A liver storage device, comprising:

the container body is used for containing perfusate; and

a breathing bladder disposed within the container body, the breathing bladder for supporting an isolated organ.

2. The liver storage device of claim 1, wherein the breathing bag is provided with at least one aperture at a middle portion thereof.

3. The liver storage device of claim 2, wherein the breathing bag comprises a ring-shaped bag body, a hollow main prism body and a plurality of hollow branch bodies; the annular bag body is arranged around the peripheries of the main prism body and the plurality of branch bodies, two ends of the main prism body are respectively communicated with the annular bag body, the main prism body is communicated with the branch bodies, the branch bodies are arranged at intervals along the main prism body in sequence, and the adjacent branch bodies, the main prism body and the annular bag body are surrounded to form the hole.

4. The liver storage device of claim 1, wherein the breathing air bag is provided with an air tube, and the air tube is used for connecting with an external control air source.

5. The liver storage device of claim 1, wherein the container body comprises a bottom plate and a side plate connected with the bottom plate, the side plate is arranged around the bottom plate, a concave plate is arranged in the middle of the bottom plate, a liquid outlet is arranged on the concave plate, and the breathing air bag is arranged in the concave plate.

6. The liver storage device of claim 5, wherein the concave plate is provided with a liquid guiding groove at a bottom portion thereof, the liquid outlet is disposed on a bottom wall of the liquid guiding groove, the bottom wall of the liquid guiding groove is an inclined wall which is obliquely disposed, the liquid outlet is disposed at a lower portion of the inclined wall, and a liquid outlet joint is disposed at the liquid outlet.

7. A liver storage device according to claim 5, wherein the concave panels are semi-elliptical or semi-spherical panels.

8. The liver storage device of claim 7, wherein the respiration air bag is elliptical, and the size of the outermost elliptical curve of the respiration air bag is matched with the size of the cross-section elliptical curve of the elliptical curved surface of the concave panel.

9. The liver storage device of any one of claims 1 to 8, further comprising a moisture retention membrane and a cover, wherein the moisture retention membrane is disposed inside the container body and is configured to be disposed above the isolated organ; the lid is provided to the mouth of the container body so as to be openable.

10. An isolated organ perfusion system, comprising the liver storage device as claimed in any one of claims 1 to 9, and further comprising a consumable pipeline, wherein the liquid outlet of the liver storage device is communicated with the liquid inlet end of the consumable pipeline, and the liquid outlet end of the consumable pipeline is used for being communicated with an isolated organ inside the liver storage device.