CN116195577B - Mechanical perfusion system for organ in-vitro culture and regeneration - Google Patents
Mechanical perfusion system for organ in-vitro culture and regeneration Download PDFInfo
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- CN116195577B CN116195577B CN202310170168.6A CN202310170168A CN116195577B CN 116195577 B CN116195577 B CN 116195577B CN 202310170168 A CN202310170168 A CN 202310170168A CN 116195577 B CN116195577 B CN 116195577B
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0247—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
Abstract
The invention discloses a mechanical perfusion system for in-vitro culture and regeneration of organs, which belongs to the field of medical equipment, wherein an organ pad is used for bearing the organs, an organ on the organ pad is lifted up by simulating the fluctuation of an air sac to simulate the influence of a lung and a diaphragm on the organs in vivo, a camera monitors the shape and the color change of the organs in a bin body and is in communication connection with a control system, and an oxygenation structure controls the temperature, the blood oxygen level and the acid-base degree of perfusate; the dialysis structure removes waste products generated by organ metabolism and maintains osmotic pressure in the system; the flow control structure controls perfusate in the blood reservoir to enter an arterial circuit, a venous circuit of an organ and a dialysis circuit of the dialysis structure respectively, and controls ascites to enter the blood reservoir; the blood gas analysis structure is arranged on the pipeline to monitor the metabolism and the functional state of the organ and is in communication connection with the control system, and the control structure is in communication connection with the organ bin, the oxygenation structure, the dialysis structure, the flow control structure and the blood gas analysis structure to display the work of the perfusion system and automatically adjust the work of the perfusion system according to preset data.
Description
Technical Field
The invention relates to the field of medical instruments, in particular to a mechanical perfusion system for organ in-vitro culture and regeneration.
Background
Organ transplantation refers to the surgical transfer of a viable donor organ into a patient to replace an organ that has lost function. At present, most organs in the human body can be transplanted, such as heart, lung, liver, kidney, pancreas, small intestine and the like. In addition, the transplantation of tissues such as cornea and blood vessel is also very mature.
The liver donor used for liver transplantation is very short, and meanwhile, a plurality of marginal livers and residual livers are discarded because the liver donor does not meet the transplantation standard. If the method can repair and regenerate the edge liver and the residual liver, so that the edge liver and the residual liver can be restored to the state and the size meeting the transplantation, the time for the patient to wait for the liver source can be greatly shortened, and the hope of healing is improved. The method of mechanical perfusion can provide oxygen, nutrition and the like for the liver in vitro, so that the liver maintains a certain activity in vitro, however, the liver survival environment of the existing perfusion system is greatly different from the liver survival environment in vivo, the problems of hemolysis, tissue necrosis, metabolic waste accumulation and the like can be possibly brought to the liver, long-time in vitro maintenance cannot be realized, and therefore, effective repair and regeneration are difficult to carry out.
Disclosure of Invention
In order to overcome the defects of the prior art, one of the purposes of the invention is to provide a mechanical perfusion system for in-vitro culture and regeneration of organs, provide a perfusion state close to the internal environment of a human body for a donor liver, detect and record various functions related to liver activity and functions, and intelligently regulate, repair and regenerate the liver.
One of the purposes of the invention is realized by adopting the following technical scheme:
the mechanical perfusion system for the in-vitro culture and regeneration of the organ comprises a pipeline and an organ bin, the mechanical perfusion system for the in-vitro culture and regeneration of the organ also comprises an oxygenation structure, a dialysis structure, a flow control structure, a blood gas analysis structure and a control system, the oxygenation structure, the dialysis structure, the flow control structure and the blood gas analysis structure are communicated with an organ in the organ bin through the pipeline, the organ bin comprises a bin body, a camera, an organ pad and a simulated air bag, the organ pad is mounted on the bin body and is used for bearing the organ, the simulated air bag is fluctuated to jack up the organ on the organ pad to simulate the influence of a lung and a diaphragm on the organ in vivo, the camera monitors the shape and the color change of the organ in the bin body and is in communication connection with the control system, and the oxygenation structure controls the temperature, the blood oxygen level and the acid-base degree of perfusate; the dialysis structure removes waste products generated by organ metabolism and maintains osmotic pressure in the system; the flow control structure comprises a blood reservoir, the flow control structure controls perfusate in the blood reservoir to enter an arterial circuit, a venous circuit and a dialysis circuit of the dialysis structure respectively, the blood gas analysis structure is arranged on the pipeline to monitor metabolism and functional states of the organ and is in communication connection with the control system, and the control structure is in communication connection with the organ bin, the oxygenation structure, the dialysis structure, the flow control structure and the blood gas analysis structure to display the operation of the perfusion system and automatically adjust the operation of the perfusion system according to preset data.
Further, the bin body is of a hollow sandwich structure, and the temperature in the bin body is maintained at 35-38 ℃ through circulating hot water.
Further, the oxygenation structure comprises a gas circuit control assembly, an arterial assembly and a venous assembly, wherein the gas circuit control assembly is respectively communicated with the arterial assembly and the venous assembly, and the gas circuit control assembly outputs mixed gas of oxygen, nitrogen and carbon dioxide and is respectively combined with perfusate of the arterial assembly and the venous assembly, so that the oxygen content of arterial perfusate is higher than that of venous perfusate.
Further, the arterial assembly comprises an arterial temperature sensor, an arterial water heating heat exchanger and an arterial oxygenator, wherein the arterial water heating heat exchanger is used for adjusting the temperature of arterial perfusate, the arterial oxygenator is used for injecting gas of the gas circuit control assembly into the arterial perfusate, and the arterial temperature sensor is used for monitoring the temperature of the arterial perfusate.
Further, the venous assembly comprises a venous water heating heat exchanger, a venous temperature sensor and a venous oxygenator, wherein the venous water heating heat exchanger is used for adjusting the temperature of venous perfusate, the venous oxygenator is used for injecting gas of the gas circuit control assembly into the venous perfusate, and the venous temperature sensor is used for monitoring the temperature of the venous perfusate.
Further, the dialysis structure comprises a dialysis column, a peristaltic pump set and a liquid storage bag which are communicated with each other, wherein the peristaltic pump set pumps the dialysis liquid in the liquid storage bag to the dialysis column and generates trans-membrane substance exchange with the perfusion liquid in the pipeline.
Further, the flow control structure further comprises a venous pressure sensor, an arterial pressure sensor and a portal venous pressure sensor, wherein the venous pressure sensor is arranged on a venous input pipe of the organ bin to monitor organ venous pressure, the arterial pressure sensor is arranged on an arterial input pipe of the organ bin to monitor organ arterial pressure, and the portal venous pressure sensor is arranged on an output pipe of the organ bin to monitor output pipe pressure.
Further, the flow control structure further comprises a second pinch valve, a third pinch valve and a medicament injection pump set, wherein the second pinch valve and the medicament injection pump set are arranged on the vein input tube, and the third pinch valve is arranged on the output pipeline.
Further, the flow control structure further comprises a dialysis circuit flow sensor, a venous flow sensor and an arterial flow sensor, wherein the arterial flow sensor is arranged on the arterial input pipe to monitor arterial flow, the venous flow sensor is arranged on the venous input pipe to monitor venous flow, and the dialysis circuit flow sensor is arranged on the output pipeline of the organ bin to monitor output pipeline flow.
Further, the blood gas analysis structure comprises a glucose sensor, a hematocrit detector and a pH sensor, and the glucose sensor, the hematocrit detector and the pH sensor are arranged on a pipeline to detect organ metabolism.
Compared with the prior art, the organ bin of the mechanical perfusion system for in-vitro culture and regeneration of the organs comprises the organ pad which is arranged on the bin body and used for bearing the organs, the organs on the organ pad are simulated to simulate the influence of lungs and diaphragm membranes on the organs in vivo by the simulated air bags which are fluctuated and extruded, the camera monitors the shape and the color change of the organs in the bin body and is in communication connection with the control system, and the oxygenation structure controls the temperature, the blood oxygen level and the degree of acid and alkali of perfusate; the dialysis structure removes waste products generated by organ metabolism and maintains osmotic pressure in the system; the flow control structure comprises a blood storage device, the flow control structure controls perfusate in the blood storage device to enter an arterial circuit, a venous circuit and a dialysis circuit of a dialysis structure of an organ respectively, the blood gas analysis structure is arranged on a pipeline to monitor metabolism and functional states of the organ and is in communication connection with the control system, the control structure is in communication connection with an organ bin, an oxygenation structure, the dialysis structure, the flow control structure and the blood gas analysis structure to display the work of the perfusion system and automatically adjust the work of the perfusion system according to preset data, through the design, the perfusion state close to the internal environment of a human body can be provided for a donor liver, various functions related to liver activity and functions are detected and recorded, and the liver can be intelligently adjusted, repaired and regenerated.
Drawings
FIG. 1 is a schematic diagram of a mechanical perfusion system for in vitro culture and regeneration of an organ according to the invention;
FIG. 2 is a schematic diagram of the structure of an organ chamber of the mechanical perfusion system for in vitro culture and regeneration of organs of FIG. 1;
FIG. 3 is a schematic structural view of an oxygenation assembly of the mechanical perfusion system of FIG. 1 for organ in vitro culture and regeneration;
FIG. 4 is a schematic diagram of the dialysis assembly of the mechanical perfusion system for in vitro culture and regeneration of an organ of FIG. 1;
FIG. 5 is a schematic view of a partial structure of the mechanical perfusion system for in vitro culture and regeneration of an organ of FIG. 1;
FIG. 6 is a schematic diagram of the ascites control of the mechanical perfusion system for in vitro organ culture and regeneration according to the present invention;
FIG. 7 is a schematic representation of the periodic volume change of a membrane lung balloon of the mechanical perfusion system for organ in vitro culture and regeneration of the present invention.
In the figure: 100. a pipeline; 101. a connecting pipe; 102. a filter; 200. an organ bin; 210. a bin body; 201. a main body; 202. an observation cover; 203. a camera; 204. an organ pad; 205. simulating an air bag; 206. an air bag control assembly; 207. a secretion collection bag; 208. weighing secretion; 300. an oxygenation structure; 301. the gas circuit control assembly; 310. an arterial component; 302. an arterial temperature sensor; 303. an arterial water heating heat exchanger; 304. an arterial oxygenator; 305. a first pinch valve; 320. a venous assembly; 306. a venous water heating heat exchanger; 307. a venous temperature sensor; 308. a venous oxygenator; 400. a dialysis structure; 401. a dialysis column; 402. peristaltic pump set; 403. a liquid storage bag; 500. a flow control structure; 501. a second pinch valve; 502. a medicament injection pump set; 503. a centrifugal pump; 504. a liquid level detection sensor; 505. a blood reservoir; 506. a dialysis circuit flow sensor; 507. a nutrient injection pump set; 508. a venous flow sensor; 509. a venous pressure sensor; 510. an arterial flow sensor; 511. an arterial pressure sensor; 512. a peristaltic pump; 513. a portal vein pressure sensor; 514. a portal vein flow sensor; 515. a third pinch valve; 600. a blood gas analysis structure; 601. a glucose sensor; 602. a red blood cell packed volume detector; 603. a pH sensor; 700. a control system; 701. a display; 702. a controller; 800. an organ.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or be present as another intermediate element through which the element is fixed. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1 to 5, a mechanical perfusion system for in vitro culture and regeneration of organs provides a perfusion state of a donor organ 800 close to the in vivo environment of a human body, detects and records various functions related to the activity and function of the organ 800, and can intelligently regulate and repair and regenerate the organ 800.
The mechanical perfusion system for in vitro culture and regeneration of organs includes a tubing 100, an organ chamber 200, an oxygenation structure 300, a dialysis structure 400, a flow control structure 500, a blood gas analysis structure 600, and a control system 700.
The pipeline 100 comprises a connecting pipe 101 and a filter 102, wherein the connecting pipe 101 is a plurality of medical-specification hoses, pipeline connecting pieces and sampling ports, and is used for connecting all structural components in an instrument, and hoses with different pipe diameters are selected according to the size of the organ 800, and the general inner diameter specification is defined by and not limited to; 1/8,1/4,3/8,1/2, the hose material is typically, but not limited to, a class III compliant material such as PVC and medical silicone. The number of filters 102 is plural, and the plural filters 102 are a leukocyte filter and a blood micro-plug filter, respectively.
Organ chamber 200 includes chamber body 210, camera 203, organ pad 204, simulated balloon 205, balloon control assembly 206, secretion collection bag 207, and secretion scale 208. The cartridge body 210 is used for accommodating the organ 800, and the cartridge simulates the environment of the organ 800 in the abdominal cavity. Specifically, the cartridge body 210 includes a main body 201 and a viewing cover 202. The side wall and the bottom of the main body 201 are hollow interlayers, the lower part of one side is provided with a water inlet, the upper part is provided with a water outlet, and circulating hot water can be pumped in to maintain the temperature in the bin at 35-38 ℃, preferably 37 ℃. The lines for venous inflow (PV), arterial inflow (HA) and venous outflow (VC) are connected to the blood vessels of the organ 800 by means of a tube connection. A viewing cover 202 is placed over the main body 201 for keeping the cabin clean, moist and warm. A camera 203 is positioned above the viewing cover 202 for monitoring and recording changes in the appearance and color of the organ 800. Organ pad 204 is made of a flexible material. Preferably, the organ pad 204 is made of silica gel, the organ pad 204 is hung in the bin 210, the edge of the organ pad 204 is fixed at the edge of the bin 210, and is used for flexibly supporting the organ 800, and holes are formed in the organ pad 204, so that ascites secreted by the organ 800 can flow out. The simulated balloon 205 is positioned below the organ pad 204 and is connected to an out-of-cabin balloon control assembly 206 by a pipeline, and the balloon control assembly 206 comprises a two-position three-way switch valve, a gas cylinder or a gas pump. The simulated balloon 205 is inflated and deflated according to certain rules and periods under the action of the balloon control assembly 206, so that the organ 800 on the organ pad 204 is jacked up and put down, the contact position of the organ 800 and the organ pad 204 is changed, and the influence of the lung and the diaphragm on the organ 800 in the body is simulated. The secretions of the organ 800 (bile in the gall bladder in this embodiment) are connected by tubing to the secretion collection bag 207 and the production of the secretions is measured by the secretion scale 208.
The oxygenation structure 300 is used to maintain blood oxygen levels and the degree of alkalinity and acidity in the organ 800 and perfusate system. The oxygenation structure 300 includes a pneumatic control assembly 301, an arterial assembly 310, a first pinch valve 305, and a venous assembly 320. The arterial assembly 310 includes an arterial temperature sensor 302, an arterial water heating heat exchanger 303, an arterial oxygenator 304. The venous assembly 320 includes a venous water heating heat exchanger 306, a venous temperature sensor 307, and a venous oxygenator 308. The gas circuit control component 301 outputs a mixed gas of oxygen, nitrogen and carbon dioxide, and the gas is combined with arterial perfusate through the arterial oxygenator 304, enters the venous oxygenator 308 through the pinch valve 305 and is combined with venous perfusate, so that the oxygen content of the artery is higher than that of a vein. After being heated by the arterial water heating heat exchanger 303 and the venous water heating heat exchanger 306, the water exchanges heat with perfusion fluid in the arterial oxygenator 304 and the venous oxygenator 308 respectively, the heated perfusion fluid also detects the temperature through the arterial temperature sensor 302 and the venous water heating heat exchanger 306 respectively, and when the temperature is not in a perfusion demand range, the power of the heat exchanger is regulated through the controller 702.
The dialysis structure 400 comprises a dialysis column 401, peristaltic pump sets 402 and a reservoir bag 403. The reservoir bag 403 includes a dialysate bag and a waste liquid bag. The dialysis structure 400 is primarily used to remove metabolic waste products generated in the system, maintaining osmotic pressure in the system. Peristaltic pump set 402 pumps the dialysate in the dialysate bag to dialysis column 401 and exchanges transmembrane material with the perfusate in the tubing before entering the waste bag. Downstream of the dialysis column 401 there is a dialysate sampling port.
The flow control structure 500 includes a second pinch valve 501, a drug injection pump set 502, a centrifugal pump 503, a fluid level detection sensor 504, a blood reservoir 505, a dialysis circuit flow sensor 506, a nutrient injection pump set 507, a venous flow sensor 508, a venous pressure sensor 509, an arterial flow sensor 510, an arterial pressure sensor 511, a peristaltic pump 512, a portal venous pressure sensor 513, a portal venous flow sensor 514, and a third pinch valve 515.
The centrifugal pump 503 drives the perfusate in the blood reservoir 505 into the arterial, venous and dialysis circuits, respectively, and the centrifugal pump 503 provides pressure pulsations under the command of the controller 702 that conform to the body's beat-to-beat pumping rules. The pressure and the pressure pulsation degree of the three-way flow are regulated by the change of the pipe diameter and the action of the second pinch valve 501. Since the organ 800 and perfusate may change during perfusion, the change in pressure is monitored by the venous pressure sensor 509, arterial pressure sensor 511 and portal pressure sensor 513, and if the pressure range is exceeded, the pressure in the flow path may be adjusted by adjusting the second pinch valve 501, third pinch valve 515 and/or adjusting the injection of the vasodilator or systolic agent by the agent injection pump set 502 according to the trend of the multiple changes. When the dialysis circuit flow sensor 506, the venous flow sensor 508, the arterial flow sensor 510, and the portal flow sensor 514 detect that the flow changes beyond a predetermined range, the system stops the infusion and alarms. The drug injection pump set 502 also feeds a blood glucose regulating drug when the glucose sensor 601 detects a blood glucose change, and supplements the drug to the system when the sampling port draws a sample where regulation is required. The nutrient injection pump set 507 is used to continuously supplement the system with nutrients needed for maintenance and regeneration at a regular or uniform rate. A liquid level detection sensor 504 is attached to the side wall of the blood reservoir 505 to monitor the level of the perfusate therein. Because the liquid volume source in the system is basically fixed, the liquid loss volume is mainly caused by a controllable dialysis circuit, and the sum of the ascites in the organ bin 200 and the volume of the perfusate in the blood reservoir 505 is relatively stable, the peristaltic pump 512 can be controlled to start or stop by monitoring whether the liquid volume in the blood reservoir 505 exceeds a control range, and then the aim of controlling the ascites capacity of the organ bin 200 is fulfilled. In addition, the upper end of the blood reservoir 505 is provided with a perfusion fluid adding inlet, and the lower end is provided with a perfusion fluid outlet, so that the perfusion fluid can be gradually replaced within a few minutes (the volume of the perfusion fluid in the blood reservoir 505 occupies 15-50% of the volume of the perfusion fluid in the whole pipeline 100), and the perfusion fluid replacement function is realized.
The blood gas analysis structure 600 includes a glucose sensor 601, an oxygen/carbon dioxide partial pressure sensor, a hematocrit detector 602, a pH sensor 603, and the blood gas analysis structure 600 is mainly used to monitor the metabolic and other core function states of the organ 800 and make the control system 700 respond accordingly through a control program.
The control system 700 includes a display 701 and a controller 702. The data monitored by the sensors and the cameras 203 in the system can be transmitted to the display 701 through the controller 702, and the user can observe, record, review and remotely view the process of interest of the organ 800 in real time. The controller 702 has an artificial intelligence function and an internet of things function, can automatically analyze the culture regeneration state of the organ 800, adopts adjustment or remote alarm, and can support unattended culture regeneration work.
With continued reference to FIG. 6, because of the random nature of ascites secretion, it is not appropriate to control flow with pressure as a standard. When the organ 800 secretes ascites, the fluid control of the abdominal waterway is controlled by a combination of a fluid level sensor and peristaltic pump 512. When the liquid level in the blood reservoir 505 is detected to be too low, the peristaltic pump 512 is started to avoid excessive effusion in the organ chamber 200; when too high, peristaltic pump 512 is stopped, thus ensuring that the volume of fluid in organ chamber 200 is sufficient to keep organ 800 sufficiently moist.
The camera 203 is arranged above the transparent observation cover 202 of the organ bin 200, so that the image of the organ 800 (liver) can be monitored in real time, the whole volume change, local volume mutation and color change of the organ 800 in the process of culturing and regenerating can be dynamically recorded, and the conditions of detumescence, regeneration, ischemia, necrosis, vascular rupture and the like of the organ 800 can be obtained through intelligent analysis by the uploading controller 702. The detection of "points" such as blood gas and flow parameters is upgraded to the monitoring of "faces" such as images. The dialysis column 401 downstream of the dialysis fluid circuit has a dialysis fluid sampling port for analyzing the efficiency of metabolic waste removal of the dialysis fluid circuit. The arterial road organ upstream, venous road organ downstream, bile road and ascites road are provided with sampling ports for analyzing blood gas and biochemical index components. The system can support the replacement of the perfusate, and the blood reservoir 505 is provided with an inlet and an outlet for the replacement of the perfusate, so that old perfusate is discharged while new perfusate is slowly added, and the abrupt change of components of the perfusion system can be avoided to stimulate organs. The controller 702 can make intelligent perfusion strategy adjustments or alarms based on sensor detection and image analysis. The liver condition can be monitored and judged remotely and actively by the staff, and the staff can be warned after the automatic image and data analysis of the system are received remotely.
With continued reference to fig. 7, for the inflation and deflation of the simulated balloon 205, the airflow rate is gradually slowed down as the simulated balloon 205 approaches the maximum and minimum volumes, instead of a simple constant flow start-stop control, reducing the vibration disturbance of the organ 800 due to sudden start-stop.
The foregoing examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that, for those skilled in the art, it is possible to make several modifications and improvements without departing from the concept of the present invention, which are equivalent to the above embodiments according to the essential technology of the present invention, and these are all included in the protection scope of the present invention.
Claims (10)
1. A mechanical perfusion system for in vitro culture and regeneration of an organ, comprising a pipeline and an organ bin, characterized in that: the mechanical perfusion system for in-vitro culture and regeneration of the organ further comprises an oxygenation structure, a dialysis structure, a flow control structure, a blood gas analysis structure and a control system, wherein the oxygenation structure, the dialysis structure, the flow control structure and the blood gas analysis structure are communicated with organs in the organ bin through pipelines, the organ bin comprises a bin body, a camera, an organ pad and a simulated air bag, the organ pad is hung in the bin body and is used for bearing the organ, holes are formed in the organ pad, ascites secreted by the organ can flow out, the simulated air bag is fluctuated to jack up the influence of organs on the organ pad on the organs in vivo by simulating the lung and a diaphragm, the camera monitors the shape and the color change of the organs in the bin body and is in communication connection with the control system, and the oxygenation structure controls the temperature, the blood oxygen level and the degree of acid and alkali of perfusate; the dialysis structure removes waste products generated by organ metabolism and maintains osmotic pressure in the system; the flow control structure comprises a blood reservoir, the flow control structure controls perfusate in the blood reservoir to enter an arterial circuit, a venous circuit and a dialysis circuit of the dialysis structure respectively, the blood gas analysis structure is arranged on the pipeline to monitor metabolism and functional states of the organ and is in communication connection with the control system, and the control structure is in communication connection with the organ bin, the oxygenation structure, the dialysis structure, the flow control structure and the blood gas analysis structure to display the operation of the perfusion system and automatically adjust the operation of the perfusion system according to preset data.
2. The mechanical perfusion system for the in vitro culture and regeneration of organs according to claim 1, wherein: the bin body is of a hollow sandwich structure, and the temperature in the bin body is maintained at 35-38 ℃ through circulating hot water.
3. The mechanical perfusion system for the in vitro culture and regeneration of organs according to claim 1, wherein: the oxygenation structure comprises an air passage control assembly, an arterial assembly and a venous assembly, wherein the air passage control assembly is respectively communicated with the arterial assembly and the venous assembly, and the air passage control assembly outputs mixed gas of oxygen, nitrogen and carbon dioxide and is respectively combined with perfusate of the arterial assembly and the venous assembly, so that the oxygen content of arterial perfusate is higher than that of venous perfusate.
4. A mechanical perfusion system for the in vitro culture and regeneration of organs according to claim 3, characterized in that: the arterial assembly comprises an arterial temperature sensor, an arterial water heating heat exchanger and an arterial oxygenator, wherein the arterial water heating heat exchanger is used for adjusting the temperature of arterial perfusate, the arterial oxygenator is used for injecting gas of the gas circuit control assembly into the arterial perfusate, and the arterial temperature sensor is used for monitoring the temperature of the arterial perfusate.
5. A mechanical perfusion system for the in vitro culture and regeneration of organs according to claim 3, characterized in that: the venous assembly comprises a venous water heating heat exchanger, a venous temperature sensor and a venous oxygenator, wherein the venous water heating heat exchanger is used for adjusting the temperature of venous perfusate, the venous oxygenator is used for injecting gas of the gas circuit control assembly into the venous perfusate, and the venous temperature sensor is used for monitoring the temperature of the venous perfusate.
6. The mechanical perfusion system for the in vitro culture and regeneration of organs according to claim 1, wherein: the dialysis structure comprises a dialysis column, a peristaltic pump set and a liquid storage bag which are communicated with each other, wherein the peristaltic pump set pumps the dialysis liquid in the liquid storage bag to the dialysis column and exchanges transmembrane substances with the perfusate in the pipeline.
7. The mechanical perfusion system for the in vitro culture and regeneration of organs according to claim 1, wherein: the flow control structure further comprises a venous pressure sensor, an arterial pressure sensor and a portal venous pressure sensor, wherein the venous pressure sensor is arranged on a venous input pipe of the organ bin to monitor organ vein pressure, the arterial pressure sensor is arranged on an arterial input pipe of the organ bin to monitor organ arterial pressure, and the portal venous pressure sensor is arranged on an output pipeline of the organ bin to monitor output pipeline pressure.
8. The mechanical perfusion system for the in vitro culture and regeneration of organs according to claim 7, wherein: the flow control structure further comprises a second pinch valve, a third pinch valve and a medicament injection pump set, wherein the second pinch valve and the medicament injection pump set are arranged on the vein input tube, and the third pinch valve is arranged on the output pipeline.
9. The mechanical perfusion system for the in vitro culture and regeneration of organs according to claim 7, wherein: the flow control structure further comprises a dialysis circuit flow sensor, a venous flow sensor and an arterial flow sensor, wherein the arterial flow sensor is arranged on the arterial input pipe to monitor arterial flow, the venous flow sensor is arranged on the venous input pipe to monitor venous flow, and the dialysis circuit flow sensor is arranged on the dialysis circuit monitoring output pipeline flow of the blood storage output pipeline.
10. The mechanical perfusion system for the in vitro culture and regeneration of organs according to claim 1, wherein: the blood gas analysis structure comprises a glucose sensor, a red blood cell packed volume detector and a pH sensor, wherein the glucose sensor, the red blood cell packed volume detector and the pH sensor are arranged on a pipeline to detect organ metabolic functions and perfusate oxygen carrying capacity.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105637572A (en) * | 2014-02-12 | 2016-06-01 | 奥林巴斯株式会社 | Pseudo-human body device |
| CN209473458U (en) * | 2018-08-17 | 2019-10-11 | 湖州露湖鄱生物科技有限公司 | A kind of liver room temperature machine perfusion system |
| CN111903664A (en) * | 2020-08-17 | 2020-11-10 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Isolated organ perfusion system and liver storage device |
| CN112244007A (en) * | 2020-09-27 | 2021-01-22 | 中山大学附属第一医院 | Intelligent isolated organ long-term maintenance device |
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- 2023-02-27 CN CN202310170168.6A patent/CN116195577B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105637572A (en) * | 2014-02-12 | 2016-06-01 | 奥林巴斯株式会社 | Pseudo-human body device |
| CN209473458U (en) * | 2018-08-17 | 2019-10-11 | 湖州露湖鄱生物科技有限公司 | A kind of liver room temperature machine perfusion system |
| CN111903664A (en) * | 2020-08-17 | 2020-11-10 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Isolated organ perfusion system and liver storage device |
| CN112244007A (en) * | 2020-09-27 | 2021-01-22 | 中山大学附属第一医院 | Intelligent isolated organ long-term maintenance device |
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