WO2021036742A1

WO2021036742A1 - Ex vivo organ perfusion apparatus

Info

Publication number: WO2021036742A1
Application number: PCT/CN2020/107704
Authority: WO
Inventors: 陈静瑜; 卢艳; 卫栋; 周鹏; 高霏; 张勃; 刘峰; 刘强; 杨振坤; 林祥华; 李伯贵; 魏晓磊; 钟嘉伟; 林冠金; 林炎志
Original assignee: 无锡市人民医院; 广东顺德工业设计研究院(广东顺德创新设计研究院)
Priority date: 2019-08-26
Filing date: 2020-08-07
Publication date: 2021-03-04

Abstract

The invention relates to an ex vivo organ perfusion apparatus, said ex vivo organ perfusion apparatus comprising: a housing, a perfusion assembly, a heat exchange assembly, and a controller; the perfusion assembly comprises a first powered pump; the output end of the first powered pump is used for connecting with the powered-pump head of a container to provide power to infuse the ex vivo organ with perfusate; the heat exchange assembly comprises a thermostatic box and a second powered pump; the thermostatic box is used for accommodating a heat exchange liquid; a heating element used for heating the heat exchange liquid is disposed in the thermostatic box; the thermostatic and the container are connected by means of a pipeline to form a circulating heat exchange pipeline used for heat exchange of the perfusate; the second powered pump is used for providing circulating flowing power to the heat exchange liquid; the first powered pump, the second powered pump, and the heating element all are electrically connected to the controller; the described ex vivo organ perfusion apparatus continuously injects perfusate into the ex vivo organ to achieve long-term preservation of the ex vivo organ at room temperature, improving the success rate of transplant surgery.

Description

Isolated organ perfusion device

Technical field

The present invention relates to the technical field of auxiliary medical equipment, in particular to an isolated organ perfusion device.

Background technique

Lung transplantation is the only effective treatment for end-stage lung disease. The main obstacles currently restricting the development of lung transplantation are the shortage of donor lungs, the high mortality of recipients, primary plant failure in the early postoperative period, and chronic rejection. The success or failure of the preservation of the donor lung is directly related to the success and failure of the transplant operation.

The traditional method of preservation of isolated organs is low-temperature static preservation. The low-temperature static preservation method reduces the energy consumption of the organ through low temperature, thereby prolonging the preservation time of the organ, and has the advantages of simplicity, safety, and low price.

However, a long-term low temperature environment can cause damage to tissue cells on the isolated organs, organ ischemia and other problems, thereby reducing the success rate of transplantation operations.

Summary of the invention

Based on this, it is necessary to address the above technical problems and provide an isolated organ perfusion device. By continuously infusing the isolated organ with perfusion fluid, the isolated organ can be preserved for a long time in a normal temperature environment, thereby effectively avoiding the low temperature environment. Problems such as tissue cell damage and organ ischemia have increased the success rate of transplant operations.

In order to achieve the above objective, the present invention provides an isolated organ perfusion device. The isolated organ perfusion device includes: a casing, a perfusion component, a heat exchange component, and a controller; the casing is provided with a device for accommodating the The first inner cavity of the filling assembly and the second inner cavity for accommodating the heat exchange assembly; the filling assembly includes a first power pump, and the output end of the first power pump is used to communicate with the power pump head of the container Connected to provide power to infuse the isolated organs with perfusate; the heat exchange assembly includes a thermostat and a second power pump, the thermostat is used to hold and replace the hot liquid, and the thermostat is provided with a device for heating the The heating element of the heat exchange liquid, the thermostat is connected with the container through a pipeline to form a circulating heat exchange pipeline for heat exchange of the perfusion liquid, and the second power pump is used to exchange heat to the perfusion liquid. The liquid provides circulating power, and the first power pump, the second power pump and the heating element are all electrically connected to the controller.

Compared with the background art, the above-mentioned isolated organ perfusion device has at least the following beneficial effects: firstly, the isolated organ is placed in a container, and the power pump head is connected with the isolated organ pipeline to form a circulating perfusion pipeline, and then the power pump The head is connected with the output end of the first power pump, and then the thermostat is connected with the container pipeline to form a circulating heat exchange pipeline, and then the power is turned on. The controller controls the first power pump and the second power pump to start, and the first power pump The perfusion fluid is driven to circulate in the above-mentioned circulating perfusion pipeline, so as to continuously infuse the isolated organs with the perfusion fluid. At the same time, the perfusion fluid exchanges heat with the heat exchange fluid in the container, so that the temperature of the perfusion fluid is comparable to that of the isolated organs. Appropriately, to achieve long-term preservation of isolated organs in a normal temperature environment, thereby effectively avoiding problems such as damage to tissue cells and organ ischemia caused by low-temperature environments; at the same time, the circulation of perfusate is driven by the first power pump, It can effectively remove thrombus, inflammatory factors or other foreign harmful substances in the isolated organs, thereby maintaining the functional blood vessels of the isolated organs, thereby improving such as pulmonary edema, improving the oxygenation capacity of the heart and lungs, etc., and can effectively treat the isolated organs. Carry out repair treatment to reduce the risk of postoperative graft loss, effectively improve the utilization rate of donor organs and the success rate of transplantation operations.

In one of the embodiments, the perfusion assembly further includes a first temperature detection module, the first temperature detection module is used to detect the temperature of the perfusion liquid, and the first temperature detection module is electrically connected to the controller. connection;

And/or, a second temperature detection module is provided in the thermostat, the second temperature detection module is used to detect the temperature of the heat exchange fluid, and the second temperature detection module is electrically connected to the controller .

In one of the embodiments, the isolated organ is an isolated lung, the isolated organ perfusion device further includes a breathing assembly, and a third inner cavity for accommodating the breathing assembly is also provided in the housing, so The breathing assembly includes a ventilator that is electrically connected to the controller, the ventilator is provided with an air supply interface and a return air interface, the air supply interface, the trachea of the isolated lung, and the The return air interface is connected with pipelines in turn to form a circulating breathing air path.

In one of the embodiments, the breathing assembly further includes a first battery for supplying power to the ventilator.

In one of the embodiments, the breathing assembly further includes a first operation screen for displaying and inputting respiratory function parameters, and the first operation screen is electrically connected to the controller.

In one of the embodiments, the perfusion assembly further includes a flow detection module configured to detect the flow of the perfusion liquid, and the flow detection module is electrically connected to the controller.

In one of the embodiments, the perfusion assembly further includes a second operation panel for displaying and inputting perfusion function parameters, and the second operation panel is electrically connected to the controller.

In one of the embodiments, the filling assembly further includes a second storage battery for supplying power to the first power pump.

In one of the embodiments, it further includes a communication module and a mobile terminal. The communication module is electrically connected to the controller, and the mobile terminal is electrically connected to the communication module.

In one of the embodiments, the housing is provided with an opening for the power pump head to be inserted into the first inner cavity, and the housing is also provided with an installation door, the installation door and the The first cavity position corresponds.

Description of the drawings

Figure 1 is a schematic structural diagram of an isolated organ perfusion device according to an embodiment of the present invention;

2 is a schematic diagram of the internal structure of the isolated organ perfusion device shown in FIG. 1;

Fig. 3 is a schematic diagram of the rear side structure of the isolated organ perfusion device shown in Fig. 1;

4 is a schematic diagram of the connection structure of the heat exchange components in the isolated organ perfusion device shown in FIG. 2;

Figure 5 is an exploded view of the mechanical perfusion device for isolated organs in an embodiment of the present invention;

Fig. 6 is a schematic structural diagram of a mechanical perfusion device for an isolated organ according to another embodiment of the present invention.

10. Isolated organ perfusion device, 11, housing, 111, first cavity, 112, second cavity, 113, third cavity, 114, opening, 115, installation door, 116, operating surface, 117, Respiratory function interface end face, 118, heat exchange function interface end face, 121, first power pump, 122, first temperature detection module, 123, flow detection module, 124, second operation screen, 125, second battery, 131, constant temperature Box, 1311, heating element, 1312, heat exchange fluid inlet, 1313, heat exchange fluid outlet, 1314, second temperature detection module, 1315, drain valve, 132, second power pump, 141, ventilator, 1411, air supply Interface, 1412, return air interface, 1413, oxygen inlet, 1414, air inlet, 142, first operation screen, 15, alarm module, 16, stop button, 17, infusion support rod, 20, container, 21, power Pump head, 30, oxygen source, 40, air source, 50, blood gas analyzer, 60, X-ray filming instrument.

detailed description

In order to facilitate the understanding of the present invention, the present invention will be more fully described below with reference to the relevant drawings. The preferred embodiments of the present invention are shown in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

It should be noted that when an element is referred to as being "fixed to" another element, it can be directly on the other element or a central element may also be present. When an element is considered to be "connected" to another element, it can be directly connected to the other element or an intermediate element may be present at the same time. In contrast, when an element is referred to as being "directly on" another element, there are no intervening elements. The "first" and "second" in the present invention do not represent a specific number or order, but are only used to distinguish names.

Please refer to FIG. 5, which shows the disassembled structure state of the isolated organ mechanical perfusion device in an embodiment. The above-mentioned isolated organ mechanical perfusion device can be used to treat such as isolated lungs and isolated hearts in a normal temperature environment. Plasma isolated organs are preserved for a long time and are also suitable for repairing isolated organs. The equipment includes a container 20 and an isolated organ perfusion device 10. The container 20 is used to house the isolated organ, and the container 20 includes a power pump head 21 The power pump head 21 is used to connect with the output end of the first power pump 121 of the isolated organ perfusion device 10, and the thermostat of the isolated organ perfusion device 10 communicates with the container pipeline to form a circulating heat exchange pipeline.

Please refer to Figure 6, the above-mentioned isolated organ mechanical perfusion device can also be connected to an oxygen gas source 30, an air gas source 40, a blood gas analyzer 50, and an X-ray filming instrument 60. Among them, the oxygen gas source 30 is used to supply the isolated organ The breathing component of the perfusion device 10 provides oxygen, and the oxygen gas source 30 is piped to the oxygen inlet 1413 of the breathing component; the air gas source 40 is used to provide air to the breathing component of the isolated organ perfusion device 10, and the air gas source 40 is connected to the breathing component. The air inlet 1414 of the component is connected by pipeline; the blood gas analyzer 50 is connected to the isolated organ perfusion device 10 for data interaction, and the isolated organ perfusion device 10 can analyze the data of the blood gas analyzer 50 to form an isolated organ perfusion process Curve icons of various parameters (ASAT/GOT, ALAT/GPT, GLU, LAC, PH, pO2, pCO2, HCT, NA+, CA+, K+, cl-, HCO3, etc.) to get the repair trend of isolated organs during perfusion A curve chart is formed to measure the quality of the isolated organ perfusion process; during the perfusion process of the isolated organ, the X-ray filmer 60 moves to the top of the isolated organ to take X-ray photos according to the needs of the experiment, and timely monitor and reflect the isolation. Perfusion effect of body organs.

As shown in Figures 1 to 3, an isolated organ perfusion device 10 includes: a housing 11, a perfusion component, a heat exchange component, and a controller (not shown in the drawings); The first inner cavity 111 of the filling assembly and the second inner cavity 112 for the replacement heat assembly; the filling assembly includes a first power pump 121, and the output end of the first power pump 121 is used to communicate with the power pump head 21 of the container 20 Connected to provide power to infuse the isolated organs with perfusate; the heat exchange assembly includes a constant temperature box 131 and a second power pump 132. The constant temperature box 131 is used to hold and replace the hot liquid, and the constant temperature box 131 is provided with a heat exchange liquid for heating The heating element 1311, the thermostat 131 and the container 20 are connected through a pipeline to form a circulating heat exchange pipeline for heat exchange of the perfusion liquid. The second power pump 132 is used to provide circulating power to the heat exchange liquid, and the first power The pump 121, the second power pump 132 and the heating element 1311 are all electrically connected to the controller.

In application, first place the isolated organ in the container 20, connect the power pump head 21 with the isolated organ pipeline to form a circulating perfusion pipeline, and then connect the power pump head 21 with the output end of the first power pump 121 , And then connect the thermostat 131 with the container 20 pipeline to form a circulating heat exchange pipeline, and then turn on the power, the controller controls the first power pump 121 and the second power pump 132 to start, the first power pump 121 drives the perfusion liquid in the above Circulating flow in the circulating perfusion pipeline, so as to achieve continuous perfusion of the perfusion fluid into the isolated organs. At the same time, the perfusion fluid exchanges heat with the heat exchange fluid in the container 20, so that the temperature of the perfusion fluid is suitable for the isolated organs. Long-term preservation of isolated organs under normal temperature environment can effectively avoid problems such as damage to tissue cells and organ ischemia caused by low temperature environment. At the same time, the circulation of perfusion fluid is driven by the first power pump 121, which can effectively remove Thrombus, inflammatory factors or other harmful foreign substances in the isolated organs can maintain the function of the isolated organs to keep the blood vessels unobstructed, thereby improving pulmonary edema, improving the oxygenation capacity of the heart and lungs, etc., and can repair and treat the isolated organ , Reduce the risk of postoperative graft loss, effectively improve the utilization rate of donor organs and the success rate of transplantation operations.

Specifically, the perfusion solution can be prepared by mixing DMEM/F12 medium components, albumin and Ficoll 70 in proportions.

Specifically, referring to Figures 1 and 4, the thermostat 131 is provided with a heat exchange liquid inlet 1312 and a heat exchange liquid outlet 1313. The heat exchange liquid outlet 1313, the container 20, and the heat exchange liquid inlet 1312 are connected in sequence to form the above-mentioned circulation exchange. Heat pipe.

Specifically, the heat exchange fluid may be sterile deionized water.

Specifically, please continue to refer to FIG. 4, the thermostat 131 is also provided with a drain valve 1315. After the isolated organ perfusion operation is completed, the drain valve 1315 can be opened to drain the heat exchange liquid in the thermostat 131 to avoid long-term closed storage. And cause bacteria to grow.

Specifically, the aforementioned heat exchange assembly further includes a compressor (not shown in the figure), the compressor is electrically connected to the controller, and the compressor is in communication with the thermostat 131 to cool inside the thermostat 131. When the compressor is working , The heating element 1311 does not work to cool the perfusion liquid inside the thermostat 131, so as to provide a low-temperature perfusion liquid for the isolated organs to realize the function of low-temperature perfusion.

In one embodiment, referring to FIGS. 2 and 4, the perfusion assembly further includes a first temperature detection module 122. The first temperature detection module 122 is used to detect the temperature of the perfusion liquid. The first temperature detection module 122 is electrically connected to the controller. Connection; and/or, a second temperature detection module 1314 is provided in the thermostat 131, the second temperature detection module 1314 is used to detect the temperature of the heat exchange fluid, and the second temperature detection module 1314 is electrically connected to the controller. The first temperature detection module 122 detects the temperature of the perfusion liquid in real time and feeds back the temperature data to the controller. When it detects that the temperature of the perfusion liquid deviates from the set temperature, the controller adjusts the heating power of the heating element 1311 so that The perfusion fluid returns to the set temperature to further ensure that the temperature of the perfusion fluid is suitable for the isolated organ; the second temperature detection module 1314 detects the temperature of the heat exchange fluid in real time and feeds back the temperature data to the controller. When the heat exchange is detected When the temperature of the liquid deviates from the set temperature, the controller adjusts the heating power of the heating element 1311 to restore the heat exchange liquid to the set temperature, further ensuring that the temperature of the heat exchange liquid is suitable for the isolated organ; The first temperature detection module 122 and the second temperature detection module 1314 can be installed separately or at the same time, which is not specifically limited here.

In one embodiment, referring to Figures 1 and 2, the isolated organ is an isolated lung, the isolated organ perfusion device 10 further includes a breathing assembly, and the housing 11 is also provided with a third inner cavity for accommodating the breathing assembly 113. The breathing assembly includes a ventilator 141. The ventilator 141 is electrically connected to the controller. The ventilator 141 is provided with an air supply interface 1411 and a return air interface 1412. The air supply interface 1411, the trachea of the isolated lung, and the return air interface 1412 are in turn The pipeline is connected to form a circulating breathing air path. The isolated lung needs to be perfused with perfusate and oxygen supply at the same time. After the power is turned on, the controller controls the first power pump 121 and the ventilator 141 to start at the same time, and the first power pump 121 is delivering to the isolated lungs. While infusing the fluid, the ventilator 141 is connected with the tracheal tube of the isolated lung to continuously supply oxygen to the isolated lung to keep the isolated lung undergoing recruitment exercise.

Specifically, referring to Figures 3 and 6, the ventilator 141 is also provided with an oxygen inlet 1413 and an air inlet 1414. The oxygen inlet 1413 is used to communicate with the above-mentioned oxygen source 30 pipeline, and the air inlet 1414 is used to communicate with The air source 40 is connected to the pipeline, and oxygen and air are mixed in proportion to form a mixed gas and then supplied to the isolated lungs to maintain the isolated lungs for recruitment exercise.

In one embodiment, please continue to refer to FIG. 2. The breathing assembly further includes a first storage battery (not marked in the drawings) for supplying power to the ventilator 141, wherein the first storage battery may be a lithium battery or a nickel-cadmium battery. Or nickel-hydrogen battery, in particular, to ensure continuous and stable supply of power, the first storage battery is preferably a lithium battery. In the event of a power failure or during the transfer of isolated organs, the first battery can be used as a power source to supply power to the ventilator 141 to ensure the continuous operation of the ventilator 141, thereby keeping the isolated lungs in recruitment exercise and effectively avoiding the isolated lungs. The occurrence of injury caused by the cessation of respiratory function.

In one embodiment, please continue to refer to FIG. 1, the breathing component further includes a first operation screen 142 for displaying and inputting breathing function parameters, and the first operation screen 142 is electrically connected to the controller. The user can input corresponding respiratory function parameters on the first operation screen 142 according to the needs of the experiment, and the controller controls the operation of the ventilator 141 according to the above-mentioned respiratory function parameters, which effectively improves the convenience of operation.

In one embodiment, referring to FIG. 2, the perfusion assembly further includes a flow detection module 123, which is used to detect the flow of the perfusion liquid, and the flow detection module 123 is electrically connected to the controller. The flow detection module 123 detects the flow of the perfusion fluid in real time and feeds the flow data back to the controller. The controller controls the power of the first power pump 121 according to the above flow data to control the flow of the perfusion fluid.

In one embodiment, referring to Fig. 1, the perfusion assembly further includes a second operation screen 124 for displaying and inputting parameters of the perfusion function, and the second operation screen 124 is electrically connected to the controller. The user can input corresponding perfusion function parameters on the second operation screen 124 according to the needs of the experiment, and the controller controls the operation of the first power pump 121 according to the foregoing perfusion function parameters, which effectively improves the convenience of operation.

In one embodiment, referring to FIG. 2, the filling assembly further includes a second storage battery 125 for supplying power to the first power pump 121, wherein the second storage battery 125 may be a lithium battery, a nickel-cadmium battery, or a nickel-hydrogen battery. In particular, in order to ensure the continuous and stable supply of power, the second storage battery 125 is preferably a lithium battery. In the event of a power failure or during the transfer of the isolated organ, the second battery 125 can be used as a power source to supply power to the first power pump 121 to ensure the continuous operation of the first power pump 121, thereby ensuring continuous perfusion of the isolated organ. Effectively avoid the occurrence of damage to the isolated organs due to the stop of the perfusion function.

In one embodiment, the isolated organ perfusion device 10 further includes a communication module (not shown in the drawings) and a mobile terminal (not shown in the drawings). The communication module is electrically connected to the controller, and the mobile terminal is electrically connected to the controller. The communication module is electrically connected. Among them, the communication module may be an Internet of Things module, including but not limited to WIFI module, GPRS module, 3G module, 4G module, etc. The communication module uploads the relevant data generated during the perfusion of the isolated organ to the database in the cloud server , Transplantation experts can view the above-mentioned data through a mobile terminal to facilitate regular monitoring and evaluation of the condition of the isolated organ, so as to ensure that the function of the isolated organ is effectively preserved for a long time.

Specifically, referring to FIG. 2, the isolated organ perfusion device 10 further includes an alarm module 15, and the alarm module 15 is electrically connected to the controller. When the controller recognizes a working abnormality based on the acquired parameter data, it will activate the alarm module 15 to notify the user to take corresponding measures in time to avoid damage to the isolated organs caused by abnormal working for a long time, and further improve the utilization rate of the donor organs And the success rate of transplant surgery.

Further, referring to Figure 1, the isolated organ perfusion device 10 is also provided with a stop button 16, which is electrically connected to the controller. When the alarm module 15 activates the alarm function, the user presses the stop button 16, and the controller That is, the whole machine is controlled to stop working, effectively avoiding the damage of the isolated organ due to the abnormal operation of the isolated organ perfusion device 10, and further improving the utilization rate of the donor organ and the success rate of the transplantation operation.

In one embodiment, please refer to Figures 1 and 2, the housing 11 is provided with an opening 114 for the power pump head 21 to be inserted into the first inner cavity 111, and the housing 11 is also provided with a mounting door 115. 115 corresponds to the position of the first inner cavity 111. During installation, after the power pump head 21 is penetrated into the first inner cavity 111 from the opening 114, the installation door 115 is opened, and the power pump head 21 is connected to the first power pump 121 for installation, and the operation is relatively convenient.

Specifically, please combine 1 to 3. The third inner cavity 113, the first inner cavity 111 and the second inner cavity 112 are sequentially arranged in the housing 11, and the housing 11 connects the third inner cavity 113, the first inner cavity 111 and One side surface of the second inner cavity 112 is set as the operating surface 116, wherein the first operating screen 142, the second operating screen 124, the stop button 16, the air supply interface 1411 and the return air interface 1412 are all set on the operating surface 116 close to the third In the position of the inner cavity 113, the installation door 115 is set at the position corresponding to the operation surface 116 and the first inner cavity 111. During the use of the isolated organ perfusion device 10, the user can only complete the isolation on the operation surface 116. Various control operations of the organ perfusion device 10 are very convenient; in addition, the other side of the housing 11 facing away from the operating surface 116 and the corresponding position of the third inner cavity 113 are set as the respiratory function interface end surface 117, the oxygen inlet 1413 and the air The access ports 1414 are all arranged on the end face 117 of the breathing function interface; furthermore, one side of the housing 11 close to the second cavity is set as the heat exchange function interface end face 118, and the heat exchange liquid inlet 1312 and the heat exchange liquid outlet 1313 are both arranged At the end face 118 of the heat exchange function interface. The setting positions of the operation surface 116, the breathing function interface end surface 117, and the heat exchange function interface end surface 118 on the housing 11 are only one embodiment. In actual applications, the operation surface 116 and the breathing function may also be changed according to different needs. The location of the interface end surface 117 and the heat exchange function interface end surface 118 on the housing 11 is not specifically limited here.

Further, the power pump head 21 is detachably connected to the first power pump 121. During the perfusion process, the perfusate does not come into contact with the isolated organ perfusion device 10. After the perfusion operation is completed, the power pump head 21 can be removed from the first power pump 121, and then the container 20 can be discarded for the next step. During the isolated organ perfusion operation, a new container 20 can be replaced, and the power pump head 21 and the first power pump 121 can be installed and connected, so as to avoid the replacement of the whole machine after the isolated organ perfusion is completed, and effectively reduce the use cost.

Furthermore, referring to Figs. 2 and 3, the housing 11 is also provided with an infusion support rod 17, which is used to hang bottled drugs, surgical instruments, etc., to further improve the convenience of use. In particular, the infusion support rod 17 can be a telescopic rod to adapt to different height requirements through telescopic adjustment, and further improve the convenience of use.

The technical features of the above embodiments can be combined arbitrarily. In order to make the description concise, all possible combinations of the technical features in the above embodiments are not described. However, as long as there is no contradiction in the combination of these technical features, they should be It is considered as the range described in this specification.

The above examples only express a few implementations of the present invention, and the descriptions are more specific and detailed, but they should not be interpreted as limiting the scope of the invention patent. It should be pointed out that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can be made, and these all fall within the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention should be subject to the appended claims.

Claims

An isolated organ perfusion device, characterized in that the isolated organ perfusion device comprises: a casing, a perfusion component, a heat exchange component, and a controller; the casing is provided with a second device for accommodating the perfusion component An inner cavity and a second inner cavity for accommodating the heat exchange assembly; the filling assembly includes a first power pump, and the output end of the first power pump is used to connect with the power pump head of the container to provide The power to infuse the perfusion fluid into the isolated organs; the heat exchange assembly includes a thermostat and a second power pump, the thermostat is used to hold and replace the hot liquid, and the thermostat is provided with a heat exchange liquid for heating the heat exchange liquid. A heating element, the thermostat is connected with the container through a pipeline to form a circulating heat exchange pipeline for heat exchange of the perfusion liquid, and the second power pump is used to provide a circulating flow for the heat exchange liquid The first power pump, the second power pump and the heating element are all electrically connected to the controller.
An isolated organ perfusion device according to claim 1, wherein the perfusion assembly further comprises a first temperature detection module, and the first temperature detection module is used to detect the temperature of the perfusion fluid, and the The first temperature detection module is electrically connected to the controller;

And/or, a second temperature detection module is provided in the thermostat, the second temperature detection module is used to detect the temperature of the heat exchange fluid, and the second temperature detection module is electrically connected to the controller .
The isolated organ perfusion device according to claim 1, wherein the isolated organ is an isolated lung, the isolated organ perfusion device further comprises a breathing assembly, and the housing is also provided with The third inner cavity of the breathing assembly is arranged, the breathing assembly includes a ventilator, the ventilator is electrically connected to the controller, the ventilator is provided with an air supply interface and a return air interface, and the air supply The interface, the trachea of the isolated lung, and the return air interface are sequentially connected by pipelines to form a circulatory breathing air path.
The isolated organ perfusion device according to claim 3, wherein the breathing assembly further comprises a first storage battery for supplying power to the ventilator.
An isolated organ perfusion device according to claim 4, wherein the breathing assembly further comprises a first operation screen for displaying and inputting respiratory function parameters, and the first operation screen and the controller Electrical connection.
The isolated organ perfusion device according to any one of claims 1-5, wherein the perfusion assembly further comprises a flow detection module, and the flow detection module is used to detect the flow of the perfusion fluid, so The flow detection module is electrically connected to the controller.
The isolated organ perfusion device according to any one of claims 1-5, wherein the perfusion assembly further comprises a second operation screen for displaying and inputting perfusion function parameters, and the second operation screen It is electrically connected with the controller.
An isolated organ perfusion device according to any one of claims 1-5, wherein the perfusion assembly further comprises a second storage battery for supplying power to the first power pump.
An isolated organ perfusion device according to any one of claims 1-5, further comprising a communication module and a mobile terminal, the communication module is electrically connected to the controller, and the mobile terminal is electrically connected to the The communication module is electrically connected.
An isolated organ perfusion device according to any one of claims 1-5, wherein the housing is provided with an opening for placing the power pump head into the first inner cavity, and An installation door is also provided on the housing, and the installation door corresponds to the position of the first inner cavity.