CN109852589A - 一种建兰花叶病毒株系的克隆及其转录载体构建 - Google Patents

Abstract

本发明公开了一种建兰花叶病毒株系，其全长基因组序列如SEQ ID NO:1‑10所示，其5'端亚基因组序列如SEQ ID NO:11‑21所示。本发明还公开了一种用于上述全长基因组序列和/或5'端亚基因组的体外转录载体、一种用于扩增上述全长基因组序列和/或5'端亚基因组的引物组合物及扩增方法、一种采用上述体外转录载体制备建兰花叶病毒的方法。本发明考虑自然状态下病毒株系准种的存在，通过本发明的方法获得了一种建兰花叶病毒株系的全长基因组序列及建兰花叶病毒株系的5'端亚基因组序列，其构建的载体制备的体外转录物具有侵染活性，这不仅增加了建兰花叶病毒基因组序列的丰富度，也为进一步深入研究建兰花叶病毒的基因重组、基因组功能及致病分子机理奠定了基础。

Description

一种建兰花叶病毒株系的克隆及其转录载体构建

技术领域

本发明涉及分子生物学领域，尤其涉及一种建兰花叶病毒株系，具体涉及该病毒的全长基因组序列和5'端亚基因组序列、其体外转录载体、其扩增引物和扩增方法及该病毒的制备方法。

背景技术

建兰花叶病毒(Cymbidium mosaic virus，CymMV)是芜菁黄花叶病毒目、甲型线形病毒科、马铃薯X病毒属成员，马铃薯X病毒(Potato virus X，PVX) 是同属的模式病毒。病毒体由外壳蛋白和正义单链RNA基因组(+ssRNA)组成，是兰科植物中寄主最广泛、危害最严重的病毒之一，可侵染蝴蝶兰、石斛兰、大花惠兰、文心兰等多种热带兰及国兰。建兰花叶病毒可单独或与齿兰环斑病毒 (Odontoglossum ringspot virus，ORSV)复合侵染兰花，使兰花叶片上形成褪绿条纹、凹陷的灰白斑或坏死圈斑等症状，导致植株生长不良、开花小而少，花期缩短，发病植株观赏价值和商品价值大为下降。因此，我国植物检疫部门将此病毒列为3类危险性病毒。

CymMV基因组5'端具有帽子结构，3'端具有ployA尾巴。除3'ploy(A)外长度为6203-6227nt。基因组具有5个开放式阅读框(Open Reading Frames，ORF)， ORF1编码一个大小为160kDa的RNA依赖性的RNA聚合酶(RNA-dependent RNA polymerase，RdRP)；ORF2-4编码分子量分别为26、13和10kDa的三基因连锁结构(Triple gene block，TGB)TGB1/2/3，被认为是运动蛋白；ORF5编码24kDa的外壳蛋白(Coat Protein，CP)，参与病毒RNA的包被。

尽管病毒基因组很小，但仍需编码多个蛋白来完成复制、运动、组装等功能。如CymMV仅有一个单链正义基因组，但仍需表达5个蛋白。在侵染过程中，病毒通常会产生亚基因组信使RNA(subgenomic mRNA，sg mRNA)来帮助合成各蛋白，大多sg mRNA为3'末端与模板RNA3'末端齐性(3'-coterminal)，但也有5'末端与模板RNA5'末端齐性的sg mRNA(5'-terminal sgRNA)。已知CymMV可以产生三条3'端sgRNA1/2/3：sgRNA1含TGB1/2/3及CP基因，编码TGB1蛋白；sgRNA2含TGB2/3及CP基因，编码TGB2及TGB3蛋白；sgRNA3 含CP基因，用于编码CP蛋白。

病毒准种是指RNA病毒和一些DNA病毒，在随机突变、自我复制和宿主的选择压力等共同作用下，以高度同源但又各有差别的状态存在。大多数病毒的 RNA聚合酶和DNA聚合酶缺乏3'至5'核酸外切酶活性，缺乏校正功能，从而产生一群存在不同位点变异的基因组DNA或者RNA。目前，中国、美国、新加坡、韩国、日本、印度等国的科学家分别从本国的栽培兰花中分离出CymMV，截至2018年9月，NCBI已收录的全长序列有14条。Moles等(2007)根据 85个CP核苷酸序列以及37个RdRP部分核苷酸序列构建的进化树，将CymMV 病毒序列分为A、B两个亚组。Vaughan等(2008)也将已报导的CymMV全长序列划分为A、B两个亚组。目前CymMV全长序列除美国夏威夷EF125178、 EF125179株系属于亚组B外，其它均属于亚组A。

侵染性cDNA克隆是指RNA病毒的具有侵染性的cDNA或是以病毒cDNA 为模板获得的具有侵染性的体外转录物。侵染性克隆可通过一次性合成全长 cDNA或合成片段拼接的方式获得。侵染性cDNA克隆共有两类，一类是在花椰菜花叶病毒(Cauliflower mosaicvirus，CaMV)35S启动子控制下的侵染性cDNA，载体质粒可直接机械接种，进而在植物体内高效转录，但是构建的载体在转录之前要先进入到植物细胞核，这在一定程度上降低了侵染效率；另一类是将病毒 cDNA置于T7、T3、SP6、λ(Pm)等来源于不同噬菌体的原核启动子下游，构建载体经线性化后在体外经相应聚合酶转录出有侵染活性的RNA。目前植物 RNA病毒的侵染性cDNA克隆大多为后者。RNA病毒侵染性克隆的建立，使重组DNA技术及其它相关技术在在研究中的应用成为可能，从而克服了RNA病毒难以进行遗传操作的问题。

小兰屿蝴蝶兰，又名桃红蝴蝶兰、姬蝴蝶兰等，蝴蝶兰属兰科植物，原生分布于菲律宾和中国台湾，是兰花栽培和分子生物学研究中最常用的模式植物。其基因组于2014测序完成并发表，基因组大小约为1.5Gb，基因数目约30000个。自然界生存的兰花通常被CymMV及ORSV侵染，其携带的病毒也随着寄主的传播而传播至各处。CymMV由于其基因组单链且缺乏纠错机制的特性，基因组突变率高且易发生基因组间重组，全球存在多个株系。上海辰山植物园自2015年开始从中国台湾批量获得小兰屿蝴蝶兰植株，并于温室常规培养，其携带病毒变异情况未知。

现有技术中，随着高通量测序技术的普及，越来越多的数据可以被获得，病毒序列也可以较为容易的从测序数据中分离出来。而之前只能根据已知序列设计引物，采取分段扩增或全长扩增的方法获得序列，该种方法仅能得到与已知序列相似的序列，不具有特异性；而采取分段扩增病毒株系再拼接的方法，忽略了自然状态下病毒株系准种存在的事实，不能得到真正的病毒序列；采用全长扩增的方法，常根据基因组5'端及3'端序列设计引物，不能得到亚基因组的情况；且关于CymMV亚基因组，仅有3'端三条亚基因组，尚未见5'端亚基因组的报道。

发明内容

为了克服现有技术的缺陷，本发明将从高通量数据中鉴定出的建兰花叶病毒株系(文中以ChenShan株系表示)以全长PCR扩增的方法克隆测序，提供了其全长基因组及5'端亚基因组并构建了其体外转录载体。

为实现上述目的，本发明采用如下技术方案：

本发明的第一个目的是克隆了一种建兰花叶病毒株系，其特征在于，所述病毒株系的全长基因组序列如SEQ ID NO:1-10所示。进一步地，上述建兰花叶病毒株系的全长基因组序列包含基因组变异的准种。

进一步地，上述建兰花叶病毒株系的5'端亚基因组序列如SEQ ID NO:11-21 所示。

本发明的第二个目的是提供一种转录上述建兰花叶病毒株系的全长基因组序列和/或其5'端亚基因组序列的体外转录载体。

为了进一步优化上述体外转录载体，本发明采取的措施还包括：

进一步地，所述转录载体的序列上游为T7启动子，序列下游含SmaⅠ酶切位点。

进一步地，用于构建体外转录载体的引物组包括如SEQ ID NO:22所示的正向引物和如SEQ ID NO:23所示的反向引物；其中，所述正向引物引入T7启动子序列，所述反向引物引入PolyA位点及SmaⅠ平末端酶切位点。

进一步地，所述体外转录载体采用pUC57载体。

进一步地，所述建兰花叶病毒株系的全长基因组序列的体外转录载体的序列如SEQ ID NO:24所示，所述建兰花叶病毒株系的5'端亚基因组序列的体外转录载体的序列如SEQ ID NO:25所示.

本发明的第三个目的是提供一种用于扩增上述建兰花叶病毒株系的全长基因组序列和/或其5'端亚基因组的引物组合物，其包括如SEQ ID NO:26所示的正向引物、如SEQID NO:27所示的反向引物以及Oligo(dT)引物。

本发明的第四个目的是提供一种采用上述引物组合物扩增建兰花叶病毒全长序列及其5'端亚基因组序列的方法，其包括如下步骤：

步骤1)RNA的提取及逆转录合成第一链cDNA；

对样本进行取样，采用液氮冷冻，提取RNA，利用引物Oligo(dT)反转录合成第一链cDNA；

步骤2)建兰花叶病毒序列的扩增；

以步骤1)的反转录产物为模板，PCR扩增建兰花叶病毒全长及其5'端亚基因组序列；其中，PCR扩增的引物采用SEQ ID NO:26所示的正向引物、如SEQ ID NO:27所示的反向引物以及Oligo(dT)引物；

步骤3)建兰花叶病毒序列连接至载体并测序；

将步骤2)中PCR扩增获得的目的片段经胶回收后，用连接酶连接到载体，并取适量的连接反应产物转化大肠杆菌感受态，待克隆长出后，挑取单克隆进行菌落PCR验证，选取阳性单克隆进行测序，测序序列导入软件进行手动拼接，获得病毒序列；

其中，正向引物和反向引物扩增得到如SEQ ID NO:1-10所示的建兰花叶病毒全长基因组序列；正向引物和oligo(dT)引物扩增得到如SEQ ID NO:11-21所示的建兰花叶病毒的5'端亚基因组序列。

为了进一步优化上述扩增方法，本发明采用的技术措施还包括：

进一步地，所述步骤1)采用的RNA的提取方法参照Qigen RNAeasy植物 RNA纯化试剂盒的操作手册。

进一步地，所述步骤1)中第一链cDNA的合成方法参照Taraka反转录系统操作手册。

进一步地，所述步骤2)的PCR扩增体系中采用高保真酶KOD-Plus-Neo。

进一步地，所述步骤2)的PCR扩增体系包括：10×KOD缓冲液5μL、2mM dNTPs 5μL、25mM MgSO₄ 3uL、引物各0.75μL、模板2μL、酶KOD-Plus-Neo 1μL、水32.5μL。

进一步地，所述步骤2)的PCR扩增体系的扩增程序为：94℃变性2分钟； 98℃10秒；55℃30秒，68℃4分30秒，30个循环；68℃7分钟。

进一步地，所述步骤3)中建兰花叶病毒序列连接至载体的步骤包括：依次加入：2×连接酶缓冲液10μL、pJET1.2/blunt载体1μL、CymMV回收产物0.15 pmol、T4DNA连接酶1μL，补水至20μL，混匀后，室温连接30分钟。

本发明的第五个目的是提供一种上述建兰花叶病毒株系的制备方法，其包括如下步骤：

步骤A：体外转录载体的线性化；

上述制备的体外转录载体经限制性内切酶SmaⅠ酶切后，进行琼脂糖凝胶电泳检测，确保载体被线性化完全；

步骤B：体外转录物的合成；

向步骤A中线性化完全的体外转录载体依次加入10×体外转录缓冲液、 ATP、CTP、UTP、GTP、m7G(5')ppp(5')G、线性化模板、T7RNA聚合酶，37℃水浴2小时，琼脂糖凝胶电泳检测转录物质量及浓度，获得转录的单链RNA。

进一步地，上述建兰花叶病毒株系的制备方法还包括病毒的接种步骤：将步骤B所获的转录单链RNA与等体积2×GKP缓冲液(50mM甘氨酸，30mM K₂HPO₄，1％Bentonite，1％Celite，PH9.2)混合，摩擦接种3-4周龄的本生烟叶片。

进一步地，上述建兰花叶病毒株系的制备方法还包括侵染烟草及体外转录物的侵染活性的验证步骤，包括：提取接种病毒的本生烟的总RNA，反转录获得 cDNA第一链，针对CymMV全长及5'端亚基因组共有区域设计如SEQ ID NO:28 和SEQ ID NO:29所示序列的引物组及针对CymMV 3'端CP区域设计如SEQ ID NO:30-SEQ ID NO:31所示序列的引物组，分别进行PCR扩增，验证体外转录物的侵染活性。

进一步地，所述侵染烟草及体外转录物的侵染活性的验证步骤还采用本生烟Actin基因作为本底对照，其扩增采用如SEQ ID NO:32-SEQ ID NO:33所示序列的引物组。

与现有技术相比，本发明采用上述技术方案具有以下有益效果：本发明将从小兰屿蝴蝶兰植株测序的RNA-seq高通量数据比对至建兰花叶病毒NCBI已发表全长序列，对读长覆盖度作图可知，植株所含的建兰花叶病毒株系不是任何一种已知的株系，是一种新的建兰花叶病毒株系；本发明将利用该株系基因组5'端及3'端引物PCR扩增的序列连接至pJET1.2/blunt载体并测序，获得该建兰花叶病毒株系的全长基因组序列及其准种信息；将利用该株系基因组5'端引物及 Oligo(dT)引物PCR扩增的序列连接至pJET1.2/blunt载体并测序，获得该建兰花叶病毒株系的5'端亚基因组序列；本发明将上述序列各选其一连接至pUC57载体，并在序列上游5'端添加T7启动子序列，3'端添加ployA及平末端酶切位点SmaⅠ，构建体外转录载体，体外转录物摩擦接种本生烟幼苗，RT-PCR验证该建兰花叶病毒株系全长及其5'端亚基因组均具有生物活性。

本发明考虑自然状态下病毒株系准种的存在，获得了一种建兰花叶病毒株系的全长基因组序列及其5'端亚基因组序列，其构建的体外转录载体得出的体外转录物具有侵染活性，这不仅增加了建兰花叶病毒基因组序列的丰富度，也为进一步深入研究建兰花叶病毒的基因重组、基因组功能及致病分子机理奠定了基础。

附图说明

图1是本发明一实施例中高通量RNA-seq数据比对至建兰花叶病毒已知序列的读长覆盖度图；

图2是本发明一实施例中CymMV Chenshan株系与已知株系及Chenshan株系各克隆间的亲缘关系树；其中，(A)部分表示：克隆Chenshan10与已知株系进化树；(B)部分表示：全长基因组克隆与亚基因组克隆进化树。

图3是本发明一实施例中CymMV Chenshan株系体外转录载体结构示意图；其中，(A)部分表示：CymMV株系Chenshan；(B)部分表示：CymMV株系Chenshan 5'端亚基因组。

图4是本发明一实施例中本生烟接种症状表型及RT-PCR验证结果图；其中，(A)部分表示：接种叶14dpi(days post infection)表型；(B)部分表示：14dpi 表型；(C)部分表示：RT-PCR验证结果。

具体实施方式

下面结合附图和实施例，对本发明的具体实施方式作进一步描述。以下实施例仅用于更加清楚地说明本发明的技术方案，而不能以此来限制本发明的保护范围。

实施例1-建兰花叶病毒株系序列的确定

本实施例为建兰花叶病毒株系序列的确定，其包括以下方面：

(1)高通量数据的处理；

采用Bowetie2将高通量数据比对至建兰花叶病毒14条已知全长基因组序列，利用genomeCoverageBed计算比对至建兰花叶病毒基因组各位置的读长覆盖度(ReadCoverage)。14条序列以比对位置对齐，序列缺失的位置Read Coverage 记为0。利用R中Plot函数作图，见图1。

各序列简称为CymMV，Cymbidium mosaic virus—Taiwan[AY571289.1]； Nanjing[JQ860108.1]；Korea[AF016914.1]；China[KR185347.1]；Kunming [AM055640.2]；Beijing[HQ681906.1]；India[AM055720.1]；Singapore[U62963.1]； Japan[AB197937.1]；TaiwanM2[EU314803.1]；Japan Cat[LC125633.1]；Hawaii3 [EF125180.1]；Hawaii2[EF125179.1]；Hawaii1[EF125178.1]。

由图1可见，本实施例通过高通量测序数据鉴定的CymMV株系不是任何一种已知的株系，其基因组1-1300nt更接近CymMV Nanjing株系，1300-6226nt 与CymMV Taiwan株系更类似，将其命名为CymMV ChenShan株系。

(2)RNA的提取及逆转录合成第一链cDNA；

对小兰屿蝴蝶兰花苞进行取样，采用液氮冷冻，参照Qigen RNAeasy植物 RNA纯化试剂盒(74904)操作手册提取RNA，第一条cDNA的合成参照Taraka 反转录系统操作手册利用引物Oligo(dT)合成。

(3)CymMV序列的扩增；

以上述反转录产物为模板，用PCR方法扩增CymMV，为保证序列准确性，采用高保真酶KOD-Plus-Neo(TOYOBO)扩增。

正向引物(CymMV-for,5'-GGAAAACCAAACCTCACGTCTA-3')(SEQ ID NO:26)；

反向引物(CymMV-rev,5'-TTTTATCTGGCTAAATATATTATGCCAGTA-3') (SEQ ID NO:27)或Oligo(dT)引物；

在200μL PCR反应管中依次加入：10×KOD缓冲液5μL、2mM dNTPs 5μL、 25mMMgSO₄ 3uL、引物各0.75μL、模板2μL、酶KOD-Plus-Neo 1μL、水32.5μL，混匀，离心，在PCR仪(C1000Touch，BIO-RAD公司)上完成反应；

反应参数为：94℃变性2分钟，98℃10秒，55℃30秒，68℃4分30秒， 30个循环，68℃7分钟。

(4)CymMV序列连接至载体并测序

PCR扩增获得的目的片段经胶回收后，用T4连接酶连接到pJET1.2/blunt 平末端载体(Thermo Scientific)。

在1.5mL EP管中依次加入：2×连接酶缓冲液10μL、pJET1.2/blunt载体1μL、CymMV回收产物0.15pmol、T4DNA连接酶1μL，补水至20μL，混匀后，室温连接30分钟。然后取10μL连接反应产物转化100μL DH5α大肠杆菌感受态，冰浴30分钟，42℃水浴90秒，冰浴3至5分钟后，加LB溶液1mL，37℃100 rpm/分钟摇菌半小时，涂板于LB(Amp+)平板上，待克隆长出后，挑取单克隆进行菌落PCR验证，选取阳性单克隆进行测序。测序序列导入SnapGene软件进行手动拼接，获得全长序列。

其中，CymMV-for/CymMV-rev引物对扩增片段得到10个不同序列的单克隆，其序列如SEQ ID NO:1-10所示，片段大小为6216-6226bp，分别命名为 Chenshan1-10。其中克隆Chenshan 9RdRP区域提前终止，Chenshan 1TGB3起始密码子突变，Chenshan 3CP区域移码突变。其它克隆以Chenshan10为例，氨基酸预测，74-4327nt编码RdRP蛋白，4333-5034nt编码TGB1蛋白，5009-5347nt 编码TGB2蛋白，5202-5477编码TGB3蛋白，5480-6151nt编码CP蛋白，与其它建兰花叶病毒株系基因组结构相同。其中1-1392nt Chenshan株系与Nanjing 株系最为相似，核酸相似性约99％，整体与Taiwan株系相似性最高，约97.4％。 10个克隆间核酸序列相似性99.47～99.69％。

其中，CymMV-for/oligo(dT)引物对扩增片段得到11个不同序列的单克隆，其序列如SEQ ID NO:11-21所示，片段大小均为5373bp，推测为CymMV产生的5’端亚基因组组，分别命名为Chenshan S1-11，其各克隆与全长克隆能很好的聚在一起，其中Chenshan S11与Chenshan S10的1-5373nt区域相似性100％。

(5)CymMV核酸序列亲缘树构建

将Chenshan10与NCBI收录全长核苷酸序列进行比对，构建进化树，采用马铃薯奥古巴花叶病毒(Potato aucuba mosaic Virus,PAMV)[S73580]作为外类群，采用Kimura 2-parameter模型利用邻接法(Neighbour-joining)在MEGA7中构树，bootstrap 1000次，如图2的(A)部分所示。以CymMV Taiwan株系为外类群，将克隆到的21条序列构树如图2的(B)部分所示。

实施例2-侵染性cDNA载体的构建

本实施例为实施例1中的CymMV的全长基因组序列和CymMV的5'端亚基因组的体外转录载体的构建，其采用下述引物：

正向引物引入T7启动子序列为：

aaaacgacggccagtgaattcATAATACGACTCACTATAGGGAGAGGAA(SEQ ID NO:22)

反向引物引入PolyA位点及SmaⅠ平末端酶切位点：

gcctctgcagtcgacgggcccACCCGGGTTTTTTTTTTTTTTT(SEQ ID NO:23)

分别将CymMV全长及亚基因组片段连接至载体pUC57，CymMV的全长基因组序列和CymMV的5'端亚基因组的体外转录载体的序列分别如SEQ ID NO: 24和SEQ ID NO:25所示，其结构示意图如图3所示，所构建的体外转录载体上游含T7启动子，下游具有SmaⅠ酶切位点。

实施例3-CymMV病毒RNA的制备及侵染验证

本实施例为采用实施例2所构建的体外转录载体进行CymMV病毒RNA的制备及侵染验证，其具体步骤包括：

(1)体外转录载体的线性化；

载体经限制性内切酶SmaⅠ酶切后，进行琼脂糖凝胶电泳检测，确保载体被线性化完全。

(2)体外转录物的合成；

依次在1.5mL EP管中加入，10×体外转录缓冲液2μL、100mM ATP 2μL、100mM CTP2μL、100mM UTP 2μL、20mM GTP 2μL、40mM m7G(5')ppp(5')G 4μL、线性化模板4μL、T7RNA聚合酶(NEB公司)2uL，37℃水浴2小时，琼脂糖电泳检测转录物质量及浓度。

(3)体外转录物的接种；

将转录出的RNA与等体积2×GKP缓冲液(50mM甘氨酸，30mM K₂HPO₄， 1％Bentonite，1％Celite，PH9.2)混合，摩擦接种3-4周龄的本生烟叶片，水与 2×GKP缓冲液等体积混合接种，作为接种对照，每日观察记录病症变化。接种后14天表型见图4的(A)部分和(B)部分所示，接种亚基因组本生烟与对照相比，植株生长缓慢，表现出叶片小、茎杆细等症状；接种全长基因组与对照相比，接种叶表现为卷曲，植株生长稍缓慢，亚基因组CymMV侵染植株株型偏小。

(4)CymMV侵染烟草RT-PCR验证；

对接种病毒的本生烟及Mock对照，提取植物总RNA，反转录获得cDNA 第一链，针对CymMV全长及5'端亚基因组共有区域设计引物 (CymMV-common-for:CCGGAGGACTTCAA(SEQID NO:28)； CymMV-common-rev:TTGCTAGGGTCGAGC(SEQ ID NO:29))及CymMV 3' 端CP区域设计引物(CymMV-CP-for:TGGCAATAATATTCGCCG(SEQ ID NO: 30)；CymMV-CP-rev:TTTTATCTGGCTAAATATATTATGCCAGTA(SEQ ID NO: 31))，分别进行PCR扩增，本生烟Actin基因作为本底对照 (NbACT-for:TGGACTCTGGTGATGGTGTC(SEQ ID NO:32)；NbACT-rev:CCTCCAATCCAAACACTGTA(SEQ ID NO:33))，PCR产物经琼脂糖凝胶电泳检测，结果见图4的(C)部分。RT-PCR结果显示，接种后植株均检测出 CymMV片段，可见得到的克隆具有复制和扩增活性，载体得出的体外转录物具有侵染活性。

由上述实施例可知，本发明考虑自然状态下病毒株系准种的存在，通过本发明的方法获得了一种准确的CymMV的全长基因组序列及CymMV的5'端亚基因组序列，这不仅增加了建兰花叶病毒基因组序列的丰富度，也为进一步深入研究建兰花叶病毒的基因重组、基因组功能及致病分子机理奠定了基础。

以上对本发明的具体实施例进行了详细描述，但其只作为范例，本发明并不限制于以上描述的具体实施例。对于本领域技术人员而言，任何对本发明进行的等同修改和替代也都在本发明的范畴之中。因此，在不脱离本发明的精神和范围下所作的均等变换和修改，都应涵盖在本发明的范围内。

序列表

<110> 上海辰山植物园

<120> 一种建兰花叶病毒株系的克隆及其转录载体构建

<130> IPI184241

<160> 33

<170> SIPOSequenceListing 1.0

<210> 1

<211> 6222

<212> DNA

<213> CymMV 全长基因组序列1(Cymbidium mosaic virus)

<400> 1

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccctgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg ggcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggctacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa aaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tacctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgat ctgccggaaa agcactctcg cgagattttt aacaaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt ataaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaacaaaat agccttattc ctgaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatacca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

cgatttctgc ggctggacta taacgcctta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta tcactctagt 4680

cgcacccacc gctacgggcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tccgcattat ttgtgctaaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catactaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgccata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acggggcgtc tattactatc actaactgcc 5400

ccgatcctgc agctatatta aataaagtag ccataggccc ctggcgaggg ttaagttacc 5460

acaataattg aaataatcat gggagagccc actccaactc cagctgccac ttactccact 5520

gccgacccca cttctgcacc caagttggcc gacctagctg ccattaagta ctcacctgtc 5580

acctcctcca tcgccacccc cgaagaaatc aaggccataa cccaattgtg ggttaacaac 5640

cttggcctcc ccgccgacac agtaggtacc gcggccattg acctggcccg cgcctacgct 5700

gacgtcgggg cgtccaagag tgccaccctg ctcggtttct gccctacgaa acctgatgtc 5760

cgtcgcgccg ctcttgccgc gcagatcttc gtggccaacg tcaccccccg ccagttttgc 5820

gcttactacg cgaaagtggt gtggaatctg atgctggcca ctaacgatcc gcccgccaac 5880

tgggccaagg ctggtttcca ggaggatact cggtttgccg cctttgactt cttcgatgcc 5940

gtcgattcca ctgccgcact ggaccctgct gaatggcagc gccgccctac tgaccgtgaa 6000

cgtgctgcgc actcgatcgg gaagtacggc gcccttgccc gtcagcgtat ccaaaacggc 6060

aacctcatca ccaacattgc tgaggtcact aagggccatc ttggctccac taacactctc 6120

tatgctctgc ctgcaccccc tactgaataa cgccaaactt aataaggcgt gtggttttct 6180

aaagtttgtt tccactactg gcataatata tttagccaga ta 6222

<210> 2

<211> 6220

<212> DNA

<213> CymMV 全长基因组序列2(Cymbidium mosaic virus)

<400> 2

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccctgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg ggcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggctacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tacctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgat ctgccggaaa agcactctcg cgagattttt aacaaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gtcatgaaga aagacactgg ggacgtcctt tttgtcaatt ataaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaacaaaat agccttattc ctgaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatacca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

cgatttctgc ggctggacta taacgcctta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta tcactctagt 4680

cgcacccacc gctacgggcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgctac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tccgcattat ttgtgctaaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgccata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acggggcgtc tattactatc actaactgcc 5400

ccgatcctgc agctatatta aataaagtag ccataggccc ctggcgaggg ttaagttacc 5460

acaataattg aaataatcat gggagagccc actccaactc cagctgccac ttactccact 5520

gccgacccca cttctgcacc caagttggcc gacctagctg ccattaagta ctcacctgtc 5580

acctcctcca tcgccacccc cgaagaaatc aaggccataa cccaattgtg ggttaacaac 5640

cttggcctcc ccgccgacac agtaggtacc gcggccattg acctggcccg cgcctacgct 5700

gacgtcgggg cgtccaagag tgccaccctg ctcggtttct gccctacgaa acctgatgtc 5760

cgtcgcgccg ctcttgccgc gcagatcttc gtggccaacg tcaccccccg ccagttttgc 5820

gcttactacg cgaaagtggt gtggaatctg atgctggcca ctaacgatcc gcccgccaac 5880

tgggccaagg ctggtttcca ggaggatact cggtttgccg cctttgactt cttcgatgcc 5940

gtcgattcca ctgccgcact ggaccctgct gaatggcagc gccgccctac tgaccgtgaa 6000

cgtgctgcgc actcgatcgg gaagtacggc gcccttgccc gtcagcgtat ccaaaacggc 6060

aacctcatca ccaacattgc tgaggtcact aagggccatc ttggctccac taacactctc 6120

tatgctctgc ctgcaccccc tactgaataa cgccaaactt aataaggcgt gtggttttct 6180

aaagtttgtt tccactactg gcataatata tttagccaga 6220

<210> 3

<211> 6224

<212> DNA

<213> CymMV 全长基因组序列3(Cymbidium mosaic virus)

<400> 3

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgt tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccctgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg ggcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggctacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tacctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgat ctgccggaaa agcactctcg cgagattttt aacaaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt ataaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaacaaaat agccttattc ctgaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatacca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

cgatttctgc ggctggacta taacgcctta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta tcactctagt 4680

cgcacccacc gctacgggcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgctac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tccgcattat ttgtgctaaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgccata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acggggcgtc tattactatc actaactgcc 5400

ccgatcctgc agctatatta aataaagtag ccataggccc ctggcgaggg ttaagttacc 5460

acaataattg aaataatcat gggagagccc actccaactc cagctgccac ttactccact 5520

gccgacccca cttctgcacc caagttggcc gacctagctg ccattaagta ctcacctgtc 5580

acctcctcca tcgccacccc cgaagaaatc aaggccataa cccaattgtg ggttaacaac 5640

cttggcctcc ccgccgacac agtaggtacc gcggccattg acctggcccg cgcctacgct 5700

gacgtcgggg cgtccaagag tgccaccctg ctcggtttct gccctacgaa acctgatgtc 5760

cgtcgcgccg ctcttgccgc gcagattttc gtggccaacg tcaccccccg ccagttttgc 5820

gcttactacg cgaaagtggt gtggaatctg atgctggcca ctaacgatcc gcccgccaac 5880

tgggccaagg ctggtttcca ggaggatact cggtttgccg ccttgacttc ttcgatgccg 5940

tcgattccac tgccgcactg gaccctgctg aatggcagcg ccgccctact gaccgtgaac 6000

gtgctgcgca ctcgatcggg aagtacggcg cccttgcccg tcagcgtatc caaaacggca 6060

acctcatcac caacattgct gaggtcacta agggccatct tggctccact aacactctct 6120

atgctctgcc tgcaccccct actgaataac gccaaactta ataaggcgtg tggttttcta 6180

aagtttgttt ccactactgg cataatatat ttagccagat aaaa 6224

<210> 4

<211> 6222

<212> DNA

<213> CymMV 全长基因组序列4(Cymbidium mosaic virus)

<400> 4

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga acgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccctgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg ggcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgctggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggccacc ccccttcctc ccatggaaaa gttaccatgg gacctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgccg tacctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcacatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgac ctgccggaaa agcactctcg cgagattttt aacaaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt ataaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctgaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatacca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

cgatttctgc ggctggacta taacgcctta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttaaattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta tcactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tccgcattat ttgtgctaaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgccata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acggggcgtc tattactatc actaactgcc 5400

ccgatcctgc agctatatta aataaagtag ccataggccc ctggcgaggg ttaagttacc 5460

acaataattt gaaataatca tgggagagcc cactccaact ccagctgcca cttactccac 5520

tgccgacccc acttctgcac ccaagttggc cgacctagct gccattaagt actcacctgt 5580

cacctcctcc atcgccaccc ccgaagaaat caaggccata acccaattgt gggttaacaa 5640

ccttggcctc cccgccgaca cagtaggtac cgcggccatt gacctggccc gcgcctacgc 5700

tgacgtcggg gcgtccaaga gtgccaccct gctcggtttc tgccctacga aacctgatgt 5760

ccgtcgcgcc gctcttgccg cgcagatctt cgtggccaac gtcacccccc gccagttttg 5820

cgcttactac gcgaaagtgg tgtggaatct gatgctggcc actaacgatc cgcccgccaa 5880

ctgggccaag gctggtttcc aggaggatac tcggtttgcc gcctttgact tcttcgatgc 5940

cgtcgattcc actgccgcac tggaacctgc tgaatggcag cgccgcccta ctgaccgtga 6000

acgtgctgcg cactcgatcg ggaagtacgg cgcccttgcc cgtcagcgta tccaaaacgg 6060

caacctcatc accaacattg ccgaggtcac taagggccat cttggctcca ctaacactct 6120

ctacgctctg cctgcacccc ctactgaata acgccaaact taataaggcg tgtggttttc 6180

taaagtttgt ttccactact ggcataatat atttagccag at 6222

<210> 5

<211> 6216

<212> DNA

<213> CymMV 全长基因组序列5(Cymbidium mosaic virus)

<400> 5

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgt cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccccgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg agcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggccacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actggaaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tatctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgac ctgccggaaa agcactctcg cgagattttt aaccaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt acaaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctaaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatatca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

tgatttctgc ggctggacta taacgcccta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaacg 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta ccactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tctgcattat ttgtgctgaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgcaata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acggggcgtc tattactatc actaactgcc 5400

ccgatcctgc agctatatta aataaagtag ccataggccc ctggcgaggg ttaagttacc 5460

acaataattt gaaataatca tgggagagct cactccaact ccagctgcca cctactccac 5520

tgccgacccc acttctgcac ccaagttggc cgacctagct gccattaagt actcacctgt 5580

cacctcctcc atcgccaccc ccgaagaaat caaggccata acccaattgt gggttaacaa 5640

ccttggcctc cccgccgaca cagtaggtac cgcggccatt gacctggccc gcgcctacgc 5700

tgacgtcggg gcgtccaaga gtgccaccct gctcggtttc tgccctacga aacctgatgt 5760

ccgtcgcgcc gctcttgccg cgcagatctt cgtggccaac gtcacccccc gccagttttg 5820

cgcttactac gcgaaagtgg tgtggaatct gatgctggcc actaacgatc cgcccgccaa 5880

ctgggccaag gctggtttcc aggaggatac tcggtttgcc gcctttgact tcttcgatgc 5940

cgtcgattcc actgccgcac tggaccctgc tgaatggcag cgccgcccta ctgaccgtga 6000

acgtgctgcg cactcgatcg ggaagtacgg cgcccttgcc cgtcagcgta tccaaaacgg 6060

caacctcatc accaacattg ccgaggtcac taagggccat cttggctcca ctaacactct 6120

ctatgctctg cctgcacccc ctactgaata acgccaaact taataaggcg tgtggttttc 6180

taaagtttgt ttccactact ggcataatat atttag 6216

<210> 6

<211> 6221

<212> DNA

<213> CymMV 全长基因组序列6(Cymbidium mosaic virus)

<400> 6

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgt cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccccgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg agcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggccacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actggaaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tatctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgac ctgccggaaa agcactctcg cgagattttt aaccaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt acaaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctaaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatatca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

tgatttctgc ggctggacta taacgcccta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaacg 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta ccactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tctgcattat ttgtgctgaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgcaata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acggggcgtc tattactatc actaactgcc 5400

ccgatcctgc agctatatta aataaagtag ccataggccc ctggcgaggg ttaagttacc 5460

acaataattt gaaataatca tgggagagct cactccaact ccagctgcca cttactccac 5520

tgccgacccc acttctgcac ccaagttggc cgacctagct gccattaagt actcacctgt 5580

cacctcctcc atcgccaccc ccgaagaaat caaggccata acccaattgt gggttaacaa 5640

ccttggcctc cccgccgaca cagtaggtac cgcggccatt gacctggccc gcgcctacgc 5700

tgacgtcggg gcgtccaaga gtgctaccct gctcggtttc tgccctacga aacctgatgt 5760

ccgtcgcgcc gctcttgccg cgcagatctt cgtggccaac gtcacccccc gccagttttg 5820

cgcttactac gcgaaggtgg tgtggaatct gatgctggcc actaacgatc cgcccgccaa 5880

ctgggccaag gctggtttcc aggaggatac tcggtttgcc gcctttgact tcttcgatgc 5940

cgtcgattcc actgccgcac tggaacctgc tgaatggcag cgccgcccta ctgaccgtga 6000

acgtgctgcg cactcgatcg ggaagtacgg cgcccttgcc cgtcagcgta tccaaaacgg 6060

caacctcatc accaacattg ccgaggtcac taagggccat cttggctcca ctaacactct 6120

ctatgctctg cctgcacccc ctactgaata acgccaaact taataaggcg tgtggttttc 6180

taaagtttgt ttccactact ggcataatat atttagccag a 6221

<210> 7

<211> 6225

<212> DNA

<213> CymMV 全长基因组序列7(Cymbidium mosaic virus)

<400> 7

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt cgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccctgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg ggcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt ccccccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggctacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaagg gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tacctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgat ctgccggaaa agcactctcg cgagattttt aacaaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt ataaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctgaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatacca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

cgatttctgc ggctggacta taacgcctta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta tcactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca agttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tccgcattat ttgtgctaaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgccata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acggggcgtc tattactatc actaactgcc 5400

ccgatcctgc agctatatta aataaagtag ccataggccc ctggcgaggg ttaagttacc 5460

acaataattg aaataatcat gggagagccc actccaactc cagctgccac ttactccact 5520

gccgacccca cttctgcacc caagttggcc gacctagctg ccattaagta ctcacctgtc 5580

acctcctcca tcgccacccc cgaagaaatc aaggccataa cccaattgtg ggttaacaac 5640

cttggcctcc ccgccgacac agtaggtacc gcggccattg acctggcccg cgcctacgct 5700

gacgtcgggg cgtccaagag tgccaccctg ctcggtttct gccctacgaa acctgatgtc 5760

cgtcgcgccg ctcttgccgc gcagatcttc gtggccaacg tcaccccccg ccagttttgc 5820

gcttactacg cgaaagtggt gtggaatctg atgctggcca ctaacgatcc gcccgccaac 5880

tgggccaagg ctggtttcca ggaggatact cggtttgccg cctttgactt cttcgatgcc 5940

gtcgattcca ctgccgcact ggaccctgct gaatggcagc gccgccctac tgaccgtgaa 6000

cgtgctgcgc actcgatcgg gaagtacggc gcccttgccc gtcagcgtat ccaaaacggc 6060

aacctcatca ccaacattgc tgaggtcact aagggccatc ttggctccac taacactctc 6120

tatgctctgc ctgcaccccc tactgaataa cgccaaactt aataaggcgt gtggttttct 6180

aaagtttgtt tccactactg gcataatata tttagccaga taaaa 6225

<210> 8

<211> 6226

<212> DNA

<213> CymMV 全长基因组序列8(Cymbidium mosaic virus)

<400> 8

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccccgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

atatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg agcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccagtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggccacc cccctccctc ccatggaaaa gttgccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa actgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcttacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg cgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tatctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgac ctgccggaaa agcactctcg cgagattttt aaccaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaagctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt acaaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctaaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catacttgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatatca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

tgatttctgc ggctggacta taacgcctta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta ccactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tctgcattat ttgtgctgaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgcaata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acggggcgtc tattactatc actaactgcc 5400

ccgatcctgc agctatatta aataaagtag ccataggccc ctggcgaggg ttaagttacc 5460

acaataattt gaaataatca tgggagagct cactccaact ccagctgcca cttactccac 5520

tgccgacccc acttctgcac ccaagttggc cgacctagct gccattaagt actcacctgt 5580

cacctcctcc atcgccaccc ccgaagaaat caaggccata acccaattgt gggttaacaa 5640

ccttggcctc cccgccgaca cagtaggtac cgcggccatt gacctggccc gcgcctacgc 5700

tgacgtcggg gcgtccaaga gtgctaccct gctcggtttc tgccctacga aacctgatgt 5760

ccgtcgcgcc gctcttgccg cgcagatctt cgtggccaac gtcacccccc gccagttttg 5820

cgcttactac gcgaaagtgg tgtggaatct gatgctggcc actaacgatc cgcccgccaa 5880

ctgggccaag gctggtttcc aggaggatac tcggtttgcc gcctttgact tcttcgatgc 5940

cgtcgattcc actgccgcac tggaacctgc tgaatggcag cgccgcccta ctgaccgtga 6000

acgtgctgcg cactcgatcg ggaagtacgg cgcccttgcc cgtcagcgta tccaaaacgg 6060

caacctcatc accaacattg ccgaggtcac taagggccat cttggctcca ctaacactct 6120

ctacgctctg cctgcacccc ctactgaata acgccaaact taataaggcg tgtggttttc 6180

taaagtttgt ttccactact ggcataatat atttagccag ataaaa 6226

<210> 9

<211> 6224

<212> DNA

<213> CymMV 全长基因组序列9(Cymbidium mosaic virus)

<400> 9

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccctgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg ggcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt ccccccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggctacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaagg gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tacctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgat ctgccggaaa agcactctcg cgagatttta acaaggcgca tggcttttcc 3000

aatgctattc agggagatgg cgtagctccc atgttccagc atcaacaggc aaaggacgaa 3060

acccttttca aggctaccat agacgctaga ctctctataa cacatcctga tgaaaataaa 3120

agagagtttg ccatgaagaa agacactggg gacgtccttt ttgtcaatta taaagctata 3180

atgaatctac ctcatgagcc tgttcctttt gaacctcgtc tctggaacat ctgcaaagct 3240

gaagtgcaga acacatacct agccaaaccc attgcaaatc ttatcaatgg taccttaagg 3300

caatcacccg attttccagc caataaaata gccttattcc tgaaatcgca atgggtaaag 3360

aaaattgaga aaattggagc cataccagtt aaacccggac agactattgc atcatttatg 3420

caagagactg ttatgctgta tggcaccatg gcacgctacc tccgtaagat gcggcaaaga 3480

tatcaacccg cacatatatt tatcaattgt gaaaagactc cggaggactt caataaattc 3540

atactcgaac actggtcaca caagcaagtt gcccacacta acgattttac tgcttttgac 3600

cagtcacaag atgctgccat gctccaattt gaagtcatca aagctaggta cttcaatatc 3660

cccgaggatg ttatagaagg atacatccaa attaagctca ctgctgaaat cttcctcggg 3720

acactttcca tcatgcgcct atctggtgaa gggccgacct tcgacgccaa tactgaatgc 3780

tctattgcct acaacgccac cagataccat attaatgaag acgtcaccca agtatacgcc 3840

ggtgacgaca tggctatgga ccatgtgtgc cctgagaaga agagcttcaa agctttggaa 3900

aagaaactga aattgacctc aaaacctctc tatccaaagc aaaaacctgg agactgggcc 3960

gatttctgcg gctggactat aacgccttat ggcatcatta agaatcctaa gaaacttgat 4020

gcatgcctac aattgcacac ccaactgggc gatgccgata aagtcgctag gtcatacgca 4080

ctcgatgcca aatatgctta tgatctgggc gatcgcattc atgaggttct gaatgttgat 4140

gaaatgacca gccacttcaa tgttataaga cagttgcaca aactgcatca acaagatgtg 4200

ctggtcccac ccgagactac cgtagccaca gcggtaaagt ctcaacctga cgtggaggat 4260

ctgtggctcc gtgcgcttag cttccctgac tggacggacc gcgcccaact tttaaaacag 4320

ggttaatttt gatggagctg gcgtacttag ttagattact agagcacaat aaattcgagc 4380

gcaccaatct gcccctttcc tcacccttag ttgtacacgg aatcgctggc agcggaaaat 4440

ccaccatcct cacaactttc catcaccatt acccggctca ccctatcttc tcacacagtc 4500

ccacattgct tgaccctagc aaccgcatat accaacagtg catcactact gattcggtgc 4560

cagacggtgc tatcgtagac gagtacaatt acaaagcact cgactactct cgctgtctag 4620

ccctcttcgg tgatccactc cagcttccac attcacttca accccactat cactctagtc 4680

gcacccaccg ctacggtccc aagcttacca gcctactcaa cgacctcttc cacctttcta 4740

tcacttctct tgcccccgtc gacagccttg attacgctga tccttttgct gtcgacccct 4800

ctggatttac cattgctgac gaggaagtgt acaactttgt ctctcagcaa gttcccggca 4860

ctttgttgcc gctcgatact gtaggtttag aatattctag tgttagcttt tactgcagtg 4920

atctccgacg ctgtgtcgta ctaaggccac tcagcacctt catcgccctc acccgcgcca 4980

agggcaacct caccatcttc gatttcaatg ccaggtttag ttccaccacc tgaccactcc 5040

aagtcactct tcgtccttgc tattggtata actgtggtct ccgcattatt tgtgctaaag 5100

tcccacactt ttccgattgc aggcgacaat attcaccgct tcccctccgg cggccaatat 5160

aaagacggta ctaagcagat aaactactgt ccacctactc atgctaggta cccgaaatat 5220

cctgactaca agtggcttgc cgccaccgcc gccatcgtca tccctctctg cctatatatt 5280

tcctaccatc ctggcaataa tattcgccgt atttgccctt gttgccatac atatcaccac 5340

ccctgagccc ttctgtacca tacacataga cggggcgtct attactatca ctaactgccc 5400

cgatcctgca gctatattaa ataaagtagc cataggcccc tggcgagggt taagttacca 5460

caataattga aataatcatg ggagagccca ctccaactcc agctgccact tactccactg 5520

ccgaccccac ttctgcaccc aagttggccg acctagctgc cattaagtac tcacctgtca 5580

cctcctccat cgccaccccc gaagaaatca aggccataac ccaattgtgg gttaacaacc 5640

ttggcctccc cgccgacaca gtaggtaccg cggccattga cctggcccgc gcctacgctg 5700

acgtcggggc gtccaagagt gccaccctgc tcggtttctg ccctacgaaa cctgatgtcc 5760

gtcgcgccgc tcttgccgcg cagatcttcg tggccaacgt caccccccgc cagttttgcg 5820

cttactacgc gaaagtggtg tggaatctga tgctggccac taacgatccg cccgccaact 5880

gggccaaggc tggtttccag gaggatactc ggtttgccgc ctttgacttc ttcgatgccg 5940

tcgattccac tgccgcactg gaccctgctg aatggcagcg ccgccctact gaccgtgaac 6000

gtgctgcgca ctcgatcggg aagtacggcg cccttgcccg tcagcgtatc caaaacggca 6060

accttatcac caacattgct gaggtcacta agggccatct tggctccact aacactctct 6120

atgctctgcc tgcaccccct actgaataac gccaaactta ataaggcgtg tggttttcta 6180

aagtttgttt ccactactgg cataatatat ttagccagat aaaa 6224

<210> 10

<211> 6226

<212> DNA

<213> CymMV 全长基因组序列10(Cymbidium mosaic virus)

<400> 10

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgt cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgtatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ccttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccccgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg agcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttacc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggccacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tatctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgac ctgccggaaa agcactctcg cgagattttt aaccaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt acaaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctaaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaaa tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcta atactgaatg 3780

ctctattgcc tacaacgcca ccagatatca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

tgatttctgc ggctggacta taacgcccta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccaat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta ccactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tctgcattat ttgtgctgaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgcaata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acggggcgtc tattactatc actaactgcc 5400

ccgatcctgc agctatatta aataaagtag ccataggccc ctggcgaggg ttaagttacc 5460

acaataattt gaaataatca tgggagagct cactccaact ccagctgcca cttactccac 5520

tgccgacccc acttctgcac ccaagttggc cgacctagct gccattaagt actcacctgt 5580

cacctcctcc atcgccaccc ccgaagaaat caaggccata acccaattgt gggttaacaa 5640

ccttggcctc cccgccgaca cagtaggtac cgcggccatt gacctggccc gcgcctacgc 5700

tgacgtcggg gcgtccaaga gtgctaccct gctcggtttc tgccctacga aacctgatgt 5760

ccgtcgcgcc gctcttgccg cgcagatctt cgtggccaac gtcacccccc gccagttttg 5820

cgcttactac gcgaaggtgg tgtggaatct gatgctggcc actaacgatc cgcccgccaa 5880

ctgggccaag gctggtttcc aggaggatac ccggtttgcc gcctttgact tcttcgatgc 5940

cgtcgattcc actgccgcac tggaacctgc tgaatggcag cgccgcccta ctgaccgtga 6000

acgtgctgcg cactcgatcg ggaagtacgg cgcccttgcc cgtcagcgta tccaaaacgg 6060

caacctcatc accaacattg ccgaggtcac taagggccat cttggctcca ctaacactct 6120

ctatgctctg cctgcacccc ctactgaata acgccaaact taataaggcg tgtggttttc 6180

taaagtttgt ttccactact ggcataatat atttagccag ataaaa 6226

<210> 11

<211> 5372

<212> DNA

<213> CymMV 5' 端亚基因组序列1(Cymbidium mosaic virus)

<400> 11

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccctgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccaac tattcaccgg ggcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggccacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaagg gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tacctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgat ctgccggaaa agtactctcg cgagattttt aacaaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt ataaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctgaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatacca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

cgatttctgc ggctggacta taacgcctta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac tttaaaacag 4320

ggttaatttt gatggagctg gcgtacttag ttagattact agagcacaat aaattcgagc 4380

gcaccaatct gcccctttcc tcacccttag ttgtacacgg aatcgctggc agcggaaaat 4440

ccaccatcct cacaactttc catcaccatt acccggctca ccctatcttc tcacacagtc 4500

ccacattgct tgaccctagc aaccgcatat accaacagtg catcactact gattcggtgc 4560

cagacggtgc tatcgtagac gagtacaatt acaaagcact cgactactct cgctgtctag 4620

ccctcttcgg tgatccactc cagcttccac attcacttca accccactat cactctagtc 4680

gcacccaccg ctacggtccc aagcttacca gcctactcaa cgacctcttc cacctttcta 4740

tcacttctct tgcccccgtc gacagccttg attacgctga tccttttgct gtcgacccct 4800

ctggatttac cattgctgac gaggaagtgt acaactttgt ctctcagcaa gttcccggca 4860

ctttgttgcc gctcgatact gtaggtttag aatattctag tgttagcttt tactgcagtg 4920

atctccgacg ctgtgtcgta ctaaagccac tcagcacctt catcgccctc acccgcgcca 4980

agggcaacct caccatcttc gatttcaatg ccaggtttag ttccaccacc tgaccactcc 5040

aagtcactct tcgtccttgc tattggtata actgtggtct ccgcattatt tgtgctaaag 5100

tcccacactt ttccgattgc aggcgacaat attcaccgct tcccctccgg cggccaatat 5160

aaagacggta ctaagcagat aaactactgt ccacctactc atgctaggta cccgaaatat 5220

cctgactaca agtggcttgc cgccaccgcc gccatcgtca tccctctctg cctatatatt 5280

tcctaccatc ctggcaataa tattcgccgt atttgccctt gttgccatac atatcaccac 5340

ccctgagccc ttctgtacca tacacataga cg 5372

<210> 12

<211> 5371

<212> DNA

<213> CymMV 5' 端亚基因组序列2(Cymbidium mosaic virus)

<400> 12

gaaaaccaaa cctcacgtct actccgctat ccgagcgaaa tagcagcccc agcataggca 60

atatcgcgct ccatggcccg agtacgtgat acccttgaca ggctccgtga tccgtcggtc 120

cttacctcaa ttaacgagga agcccaccgg catatccgtc ctgtccttgc ctcggcactc 180

gtaaactgcc cttacgcgtt gaccgaggaa gaggccgact gtctcgaaaa tctcggcgtg 240

acagtcaatc ccttcgccat ccaaacgcat acacatgctg cagctaaaac tgttgaaaat 300

cgcatgttgg aaattgttgg cacccacctt cctaaagaac cctccacctt catattcctt 360

aagcgaagca agcttcgcta cctacgacgc gccgccaaca acaaagacat atttcaaaat 420

caacacattg agcctaaaga cttattgcgt tacgatgacg agtcttgtgg agcaatgcca 480

gaatgctcca caagcacagc ctacatcagc gatgctctcc atttcttgag ctacgctcag 540

cttgggaaaa tattccagga ttctcccaag ctgaaaattc tgctggcaac cctggtcctc 600

cccgtggaag ccctccatag gcacccatct ctttatcctg ccatctacac tttgaactac 660

cataaggatg gctttgaata tatacccggc aatcacgccg gaggtgccta cttccacgag 720

tactccactc tgcagtggtt gactctcggg aaactaatta tcaatgatcc tttgaaagta 780

aagaaacccc ttactcttac tgtacagcta atagaaagtc taggggccaa ccatctttta 840

cttatcaccc gaggggatct tcgcacaccc aaactgcgga cattcgctaa agatacccat 900

gttctactac cacaaatatt ccatcctaag ggtatgaatg cgaataaacc attgtccaaa 960

cgtagagcta tgcagttgtg gctctacgcc aaatctgtta aagaggtgag cgaacgggat 1020

ctttatgcta aggtgaggca attgatcccg acttctgagt tagacctttt tgaccccgtg 1080

gaggtgaccc acttggtcaa ttacttacta ttcatcagtc acctttcctc cgtatcttcg 1140

tatgacgaca tcttgtcgtc taacatattc caacatttca ctattccaat caagaacaag 1200

atacgggaat tggtccagct attcaccgga gcggaccaat ttaatcaact gctcaaagct 1260

ctcgactggc aaacattttc atactctatg ccagtagaga ccatccatac acgtgcggcc 1320

aattatcaag tcgctaaaac gcttcgcatg tgcagagacc taccatgcga tgaatatgat 1380

cgtgtaaagg atgtcctcag acagctccct gacggagtga cgttgtttga ggaaaacgac 1440

aagcaagatc cctcaagccc tgaagccgag gactctgagg atgacactga ctccgtcgac 1500

ttcaaccttc ctcccactca tgacttgccc ccaaactttg atcctctaga caagggtaag 1560

tccattatcg ttgacactgg caacccctca acttctacgg cacccgccgt cacttttgct 1620

gccggtatca actcctcagc ctccacaaat atttcatttg gaagcttcac acctgaggct 1680

gaggccaccc ccctccctcc catggaaaag ttaccatggg atctttggat tcccctactg 1740

gaacaacatg gctttaaagg aaagagcaag ctgtacaagc ccacgggtga actaatctgc 1800

cccataactg aaatcaaaac agttccccac tgcccctttc ccgataaagt ccaggatggc 1860

tgtgtgcttg cgcttaaatc tattaagcgt ttcgccacaa aaatgactat gctcagttct 1920

cgagcttctg cctacacttc tgacattaag aatagtagga ctggaaagct cctacccgcc 1980

atgaacatgc cctggaaagc ctctctcgcc tatgtcactc aacatggtga tagagaaatt 2040

cctggcgtcg tgatccatgg tgccggtggc tgtggcaaat cttatgccat ccaaaaatgg 2100

ttgagaagct gctctgatcc ttgtgcagct actgtggtat gcccaactct agaactacgt 2160

aatgactggc tcaacaagat cggaagctac gaacaaacga atatcaaaac tttcgagaaa 2220

gctttaattc agccagtcaa caacgtagtt atctttgatg actacaccaa gctaccccct 2280

gggtacatcg agaccatggt gtaccatcac cacaacctcg acctgatcat actaactggg 2340

gatcctatgc agagtgccta ccatgaaact aacagggatg cctatatctc tttaatacct 2400

gatgcctctg ccatctttag tgagtactgt gagttcaaca tcaacgccac ccaccgtaat 2460

gtggctgaac tcgcttgcct cctcggcgtc tattccgaac gccaaggcaa actcactgtt 2520

tccttcagca cagccccact ttctaaaggt aaagtaccca ttttggtacc ctctagaatg 2580

aagcaagagg cttttgctga tgtcggcaac cgttgcatga cttatgccgg ttgccagggt 2640

ctcactgccc ctaaaatcca aattttgata gacaaccaca ctacgttctg ctctgaacaa 2700

acattataca cttgtctatc acgggctgtg gaccaaatcc acttcattaa cactggtccc 2760

aactcgcagg ctttctggac taaattggaa tcaacgccat atctaaaagc tttcctcgat 2820

aattaccgtg aggaacaaac tgaacggcta acatctacag ccccagagcc tgaagtccgt 2880

gagccagcag ccccaaaacg catatccctg tcgaaaatac atctggctta cgtatttctg 2940

cacttgacct gccggaaaag cactctcgcg agatttttaa ccaggcgcat ggcttttcca 3000

atgctattca gggagatggc gtagctccca tgttccagca tcaacaggca aaggacgaaa 3060

cccttttcaa ggctaccata gacgctagac tctctataac acatcctgat gaaaataaaa 3120

gagagtttgc catgaagaaa gacactgggg acgtcctttt tgtcaattac aaagctataa 3180

tgaatctacc tcatgagcct gttccttttg aacctcgtct ctggaacatc tgcaaagctg 3240

aagtgcagaa cacataccta gccaaaccca ttgcaaatct tatcaatggt accttaaggc 3300

aatcacccga ttttccagcc aataaaatag ccttattcct aaaatcgcaa tgggtaaaga 3360

aaattgagaa aattggagcc ataccagtta aacccggaca gactattgca tcatttatgc 3420

aagagactgt tatgctgtat ggcaccatgg cacgctacct ccgtaagatg cggcaaagat 3480

atcaacccgc acatatattt atcaattgtg aaaagactcc ggaggacttc aataaattca 3540

tactcgaaca ctggtcacac aagcaagttg cccacactaa cgattttact gcttttgacc 3600

agtcacaaga tgctgccatg ctccaatttg aagtcatcaa agctaggtac ttcaatatcc 3660

ccgaggatgt tatagaagga tacatccaaa ttaagctcac tgctgaaatc ttcctcggga 3720

cactttccat catgcgccta tctggtgaag ggccgacctt cgacgccaat actgaatgct 3780

ctattgccta caacgccacc agatatcata ttaatgaaga cgtcacccaa gtatacgccg 3840

gtgacgacat ggctatggac catgtgtgcc ctgagaagaa gagcttcaaa gctttggaaa 3900

agaaactgaa attgacctca aaacccctct atccaaagca aaaacctgga gactgggctg 3960

atttctgcgg ctggactata acgccctatg gcatcattaa gaatcctaag aaacttgatg 4020

catgcctaca attgcacacc caactgggcg atgccgataa agtcgctagg tcatacgcac 4080

tcgatgccaa atatgcttat gatctgggcg atcgcattca tgaggttctg aatgttgatg 4140

aaatgaccag ccacttcaat gttataagac agttgcacaa actgcatcaa caagatgtgc 4200

tggtcccacc cgagactacc gtagccacag cggtaaagtc tcaacctgac gtggaggatc 4260

tgtggctccg tgcgcttagc ttccctgact ggacggaccg cgcccaactt ttaaaacggg 4320

gttaattttg atggagctgg cgtacttagt tagattacta gagcacaata aattcgagcg 4380

caccaatctg cccctttcct cacccttagt tgtacacgga atcgctggca gcggaaaatc 4440

caccatcctc acaactttcc atcaccatta cccggctcac cctatcttct cacacagtcc 4500

cacattgctt gaccctagca accgcatata ccaacagtgc atcactactg attcggtgcc 4560

agacggtgct atcgtagacg agtacaatta caaagcactc gactactctc gctgtctagc 4620

cctcttcggt gatccactcc agcttccaca ttcacttcaa ccccactacc actctagtcg 4680

cacccaccgc tacggtccca agcttaccag cctactcaac gacctcttcc acctttctat 4740

cacttctctt gcccccgtcg acagccttga ttacgctgat ccttttgctg tcgacccctc 4800

tggatttacc attgctgacg aggaagtgta cgactttgtc tctcagcagg ttcccggcac 4860

tttgttgccg ctcgatactg taggtttaga atattctagt gttagctttt actgcagtga 4920

tctccgacgc tgtgtcgtac taaggccact cagcaccttc atcgccctca cccgcgccaa 4980

gggcaacctc accatcttcg atttcaatgc caggtttagt tccaccacct gaccactcca 5040

agtcactctt cgtccttgct attggtataa ctgtggtctc tgcattattt gtgctgaagt 5100

cccacacttt tccgattgca ggcgacaata ttcaccgctt cccctccggc ggccaatata 5160

aagacggtac taagcagata aactactgtc cacctactca tgctaggtac ccgaaatatc 5220

ctgactacaa gtggcttgcc gccaccgccg ccatcgtcat ccctctctgc ctatatattt 5280

cctaccatcc tggcaataat attcgccgta tttgcccttg ttgcaataca tatcaccacc 5340

cctgagccct tctgtaccat acacatagac g 5371

<210> 13

<211> 5373

<212> DNA

<213> CymMV 5' 端亚基因组序列3(Cymbidium mosaic virus)

<400> 13

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgt cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccccgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg agcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtggag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttacc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggccacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tatctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgac ctgccggaaa agcactctcg cgagattttt aaccaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt acaaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctaaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcta atactgaatg 3780

ctctattgcc tacaacgcca ccagatatca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

tgatttctgc ggctggacta taacgcccta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta ccactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcactt tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tctgcattat ttgtgctgaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgcaata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acg 5373

<210> 14

<211> 5373

<212> DNA

<213> CymMV 5' 端亚基因组序列4(Cymbidium mosaic virus)

<400> 14

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccccgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

atatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg agcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccagtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggccacc cccctccctc ccatggaaaa gttgccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgtcaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tatctaaaag ctttcctcga 2820

taactaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgac ctgccggaaa agcactctcg cgagattttt aaccaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaagctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt acaaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctaaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catacttgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatatca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgaaaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

tgatttctgc ggctggacta taacgcctta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccgac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta ccactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tctgcattat ttgtgctgaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgcaata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acg 5373

<210> 15

<211> 5373

<212> DNA

<213> CymMV 5' 端亚基因组序列5(Cymbidium mosaic virus)

<400> 15

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccccgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

atatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg agcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccagtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggccacc cccctccctc ccatggaaaa gttgccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg cgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgtcaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tatctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgac ctgccggaaa agcactctcg cgagattttt aaccaggcgc atggcttttc 3000

aaatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaagctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt acaaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctaaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catacttgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatatca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

tgatttctgc ggctggacta taacgcctta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta ccactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tctgcattat ttgtgctgaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgcaata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acg 5373

<210> 16

<211> 5373

<212> DNA

<213> CymMV 5' 端亚基因组序列6(Cymbidium mosaic virus)

<400> 16

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccctgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg ggcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggctacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tacctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgat ctgccggaaa agcactctcg cgagattttt aacaaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt ataaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaacaaaat agccttattc ctgaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatacca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

cgatttctgc ggctggacta taacgcctta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgcggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta tcactctagt 4680

cgcacccacc gctacgggcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgctac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tccgcattat ttgtgctaaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgccata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acg 5373

<210> 17

<211> 5373

<212> DNA

<213> CymMV 5' 端亚基因组序列7(Cymbidium mosaic virus)

<400> 17

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccctgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg ggcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt ccccccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggctacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaagg gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tacctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgat ctgccggaaa agcactctcg cgagattttt aacaaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt ataaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctgaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatacca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

cgatttctgc ggctggacta taacgcctta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta tcactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca agttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tccgcattat ttgtgctaaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgccata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acg 5373

<210> 18

<211> 5373

<212> DNA

<213> CymMV 5' 端亚基因组序列8(Cymbidium mosaic virus)

<400> 18

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgt cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccccgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg agcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggccacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actggaaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tatctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgac ctgccggaaa agcactctcg cgagattttt aaccaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt acaaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctaaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatatca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

tgatttctgc ggctggacta taacgcccta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaacg 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta ccactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tctgcagtat ttgtgctgaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgcaata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acg 5373

<210> 19

<211> 5373

<212> DNA

<213> CymMV 5' 端亚基因组序列9(Cymbidium mosaic virus)

<400> 19

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ccgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccctgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg ggcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagtaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgctggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggccacc ccccttcctc ccatggaaaa gttaccatgg gacctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctatacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct accatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacattct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgccg tacctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgttgaaaat acatctggct tacgtatttc 2940

tgcacttgac ctgccggaaa agcactctcg cgagattttt aacaaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt ataaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctgaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatacca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

cgatttctgc ggctggacta taacgcctta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgacctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttaaattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta tcactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tccgcattat ttgtgctaaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgccata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acg 5373

<210> 20

<211> 5373

<212> DNA

<213> CymMV 5' 端亚基因组序列10(Cymbidium mosaic virus)

<400> 20

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgc cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgaaga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgcatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ctttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccctgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg ggcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttgcc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgctg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggctacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tacctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgat ctgccggaaa agcactctcg cgagattttt aacaaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt ataaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctgaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaga tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacacc aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcca atactgaatg 3780

ctctattgcc tacaacgcca ccagatacca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

cgatttctgc ggctggacta taacgcctta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccgat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta tcactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tccgcattat ttgtgctaaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgccata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acg 5373

<210> 21

<211> 5373

<212> DNA

<213> CymMV 5' 端亚基因组序列11(Cymbidium mosaic virus)

<400> 21

ggaaaaccaa acctcacgtc tactccgcta tccgagcgaa atagcagccc cagcataggc 60

aatatcgcgc tccatggccc gagtacgtga tacccttgac aggctccgtg atccgtcggt 120

ccttacctca attaacgagg aagcccaccg gcatatccgt cctgtccttg cctcggcact 180

cgtaaactgc ccttacgcgt tgaccgagga agaggccgac tgtctcgaaa atctcggcgt 240

gacagtcaat cccttcgcca tccaaacgca tacacatgct gcagctaaaa ctgttgaaaa 300

tcgtatgttg gaaattgttg gcacccacct tcctaaagaa ccctccacct tcatattcct 360

taagcgaagc aagcttcgct acctacgacg cgccgccaac aacaaagaca tatttcaaaa 420

tcaacacatt gagcctaaag acttattgcg ttacgatgac gagtcttgtg gagcaatgcc 480

agaatgctcc acaagcacag cctacatcag cgatgctctc catttcttga gctacgctca 540

gcttgggaaa atattccagg attctcccaa gctgaaaatt ctgctggcaa ccctggtcct 600

ccccgtggaa gccctccata ggcacccatc tctttatcct gccatctaca ccttgaacta 660

ccataaggat ggctttgaat atatacccgg caatcacgcc ggaggtgcct acttccacga 720

gtactccact ctgcagtggt tgactctcgg gaaactaatt atcaatgatc ctttgaaagt 780

aaagaaaccc cttactctta ctgtacagct aatagaaagt ctaggggcca accatctttt 840

acttatcacc cgaggggatc ttcgcacacc caaactgcgg acattcgcta aagataccca 900

tgttctacta ccacaaatat tccatcctaa gggtatgaat gcgaataaac cattgtccaa 960

acgtagagct atgcagttgt ggctctacgc caaatctgtt aaagaggtga gcgaacggga 1020

tctttatgct aaggtgaggc aattgatccc gacttctgag ttagaccttt ttgaccccgt 1080

ggaggtgacc cacttggtca attacttact attcatcagt cacctttcct ccgtatcttc 1140

gtatgacgac atcttgtcgt ctaacatatt ccaacatttc actattccaa tcaagaacaa 1200

gatacgggaa ttggtccagc tattcaccgg agcggaccaa tttaatcaac tgctcaaagc 1260

tctcgactgg caaacatttt catactctat gccagtagag accatccata cacgtgcggc 1320

caattatcaa gtcgctaaaa cgcttcgcat gtgcagagac ctaccatgcg atgaatatga 1380

tcgtgtaaag gatgtcctca gacagctccc tgacggagtg acgttgtttg aggaaaacga 1440

caagcaagat ccctcaagcc ctgaagccga ggactctgag gatgacactg actccgtcga 1500

cttcaacctt cctcccactc atgacttacc cccaaacttt gatcctctag acaagggtaa 1560

gtccattatc gttgacactg gcaacccctc aacttctacg gcacccgccg tcacttttgc 1620

tgccggtatc aactcctcag cctccacaaa tatttcattt ggaagcttca cacctgaggc 1680

tgaggccacc cccctccctc ccatggaaaa gttaccatgg gatctttgga ttcccctact 1740

ggaacaacat ggctttaaag gaaagagcaa gctgtacaag cccacgggtg aactaatctg 1800

ccccataact gaaatcaaaa cagttcccca ctgccccttt cccgataaag tccaggatgg 1860

ctgtgtgctt gcgcttaaat ctattaagcg tttcgccaca aaaatgacta tgctcagttc 1920

tcgagcttct gcctacactt ctgacattaa gaatagtagg actgggaagc tcctacccgc 1980

catgaacatg ccctggaaag cctctctcgc ctatgtcact caacatggtg atagagaaat 2040

tcctggcgtc gtgatccatg gtgccggtgg ctgtggcaaa tcttatgcca tccaaaaatg 2100

gttgagaagc tgctctgatc cttgtgcagc tactgtggta tgcccaactc tagaactacg 2160

taatgactgg ctcaacaaga tcggaagcta cgaacaaacg aatatcaaaa ctttcgagaa 2220

agctttaatt cagccagtca acaacgtagt tatctttgat gactacacca agctaccccc 2280

tgggtacatc gagaccatgg tgtaccatca ccacaacctc gacctgatca tactaactgg 2340

ggatcctatg cagagtgcct accatgaaac taacagggat gcctatatct ctttaatacc 2400

tgatgcctct gccatcttta gtgagtactg tgagttcaac atcaacgcca cccaccgtaa 2460

tgtggctgaa ctcgcttgcc tcctcggcgt ctattccgaa cgccaaggca aactcactgt 2520

ttccttcagc acagccccac tttctaaagg taaagtaccc attttggtac cctctagaat 2580

gaagcaagag gcttttgctg atgtcggcaa ccgttgcatg acttatgccg gttgccaggg 2640

tctcactgcc cctaaaatcc aaattttgat agacaaccac actacgttct gctctgaaca 2700

aacattatac acttgtctat cacgggctgt ggaccaaatc cacttcatta acactggtcc 2760

caactcgcag gctttctgga ctaaattgga atcaacgcca tatctaaaag ctttcctcga 2820

taattaccgt gaggaacaaa ctgaacggct aacatctaca gccccagagc ctgaagtccg 2880

tgagccagca gccccaaaaa cgcatatccc tgtcgaaaat acatctggct tacgtatttc 2940

tgcacttgac ctgccggaaa agcactctcg cgagattttt aaccaggcgc atggcttttc 3000

caatgctatt cagggagatg gcgtagctcc catgttccag catcaacagg caaaggacga 3060

aacccttttc aaggctacca tagacgctag actctctata acacatcctg atgaaaataa 3120

aagagagttt gccatgaaga aagacactgg ggacgtcctt tttgtcaatt acaaagctat 3180

aatgaatcta cctcatgagc ctgttccttt tgaacctcgt ctctggaaca tctgcaaagc 3240

tgaagtgcag aacacatacc tagccaaacc cattgcaaat cttatcaatg gtaccttaag 3300

gcaatcaccc gattttccag ccaataaaat agccttattc ctaaaatcgc aatgggtaaa 3360

gaaaattgag aaaattggag ccataccagt taaacccgga cagactattg catcatttat 3420

gcaagagact gttatgctgt atggcaccat ggcacgctac ctccgtaaaa tgcggcaaag 3480

atatcaaccc gcacatatat ttatcaattg tgaaaagact ccggaggact tcaataaatt 3540

catactcgaa cactggtcac acaagcaagt tgcccacact aacgatttta ctgcttttga 3600

ccagtcacaa gatgctgcca tgctccaatt tgaagtcatc aaagctaggt acttcaatat 3660

ccccgaggat gttatagaag gatacatcca aattaagctc actgctgaaa tcttcctcgg 3720

gacactttcc atcatgcgcc tatctggtga agggccgacc ttcgacgcta atactgaatg 3780

ctctattgcc tacaacgcca ccagatatca tattaatgaa gacgtcaccc aagtatacgc 3840

cggtgacgac atggctatgg accatgtgtg ccctgagaag aagagcttca aagctttgga 3900

aaagaaactg aaattgacct caaaacctct ctatccaaag caaaaacctg gagactgggc 3960

tgatttctgc ggctggacta taacgcccta tggcatcatt aagaatccta agaaacttga 4020

tgcatgccta caattgcaca cccaactggg cgatgccaat aaagtcgcta ggtcatacgc 4080

actcgatgcc aaatatgctt atgatctggg cgatcgcatt catgaggttc tgaatgttga 4140

tgaaatgacc agccacttca atgttataag acagttgcac aaactgcatc aacaagatgt 4200

gctggtccca cccgagacta ccgtagccac agcggtaaag tctcaacctg acgtggagga 4260

tctgtggctc cgtgcgctta gcttccctga ctggacggac cgcgcccaac ttttaaaaca 4320

gggttaattt tgatggagct ggcgtactta gttagattac tagagcacaa taaattcgag 4380

cgcaccaatc tgcccctttc ctcaccctta gttgtacacg gaatcgctgg cagcggaaaa 4440

tccaccatcc tcacaacttt ccatcaccat tacccggctc accctatctt ctcacacagt 4500

cccacattgc ttgaccctag caaccgcata taccaacagt gcatcactac tgattcggtg 4560

ccagacggtg ctatcgtaga cgagtacaat tacaaagcac tcgactactc tcgctgtcta 4620

gccctcttcg gtgatccact ccagcttcca cattcacttc aaccccacta ccactctagt 4680

cgcacccacc gctacggtcc caagcttacc agcctactca acgacctctt ccacctttct 4740

atcacttctc ttgcccccgt cgacagcctt gattacgctg atccttttgc tgtcgacccc 4800

tctggattta ccattgctga cgaggaagtg tacaactttg tctctcagca ggttcccggc 4860

actttgttgc cgctcgatac tgtaggttta gaatattcta gtgttagctt ttactgcagt 4920

gatctccgac gctgtgtcgt actaaggcca ctcagcacct tcatcgccct cacccgcgcc 4980

aagggcaacc tcaccatctt cgatttcaat gccaggttta gttccaccac ctgaccactc 5040

caagtcactc ttcgtccttg ctattggtat aactgtggtc tctgcattat ttgtgctgaa 5100

gtcccacact tttccgattg caggcgacaa tattcaccgc ttcccctccg gcggccaata 5160

taaagacggt actaagcaga taaactactg tccacctact catgctaggt acccgaaata 5220

tcctgactac aagtggcttg ccgccaccgc cgccatcgtc atccctctct gcctatatat 5280

ttcctaccat cctggcaata atattcgccg tatttgccct tgttgcaata catatcacca 5340

cccctgagcc cttctgtacc atacacatag acg 5373

<210> 22

<211> 49

<212> DNA

<213> 构建体外转录载体的正向引物(Artificial Sequence)

<400> 22

aaaacgacgg ccagtgaatt cataatacga ctcactatag ggagaggaa 49

<210> 23

<211> 43

<212> DNA

<213> 构建体外转录载体的反向引物(Artificial Sequence)

<400> 23

gcctctgcag tcgacgggcc cacccgggtt tttttttttt ttt 43

<210> 24

<211> 6277

<212> DNA

<213> 全长基因组序列的体外转录载体(Artificial Sequence)

<400> 24

ataatacgac tcactatagg gagaggaaaa ccaaacctca cgtctactcc gctatccgag 60

cgaaatagca gccccagcat aggcaatatc gcgctccatg gcccgagtac gtgataccct 120

tgacaggctc cgtgatccgt cggtccttac ctcaattaac gaggaagccc accggcatat 180

ccgccctgtc cttgcctcgg cactcgtaaa ctgcccttac gcgttgaccg aggaagaggc 240

cgactgtctc gaaaatctcg gcgtgacagt caatcccttc gccatccaaa cgcatacaca 300

tgctgcagct aaaactgttg aaaatcgcat gttggaaatt gttggcaccc accttcctaa 360

agaaccctcc accttcatat tccttaagcg aagcaagctt cgctacctac gacgcgccgc 420

caacaacaaa gacatatttc aaaatcaaca cattgagcct aaagacttat tgcgttacga 480

tgacgagtct tgtggagcaa tgccagaatg ctccacaagc acagcctaca tcagcgatgc 540

tctccatttc ttgagctacg ctcagcttgg gaaaatattc caggattctc ccaagctgaa 600

aattctgctg gcaaccctgg tcctccccgt ggaagccctc cataggcacc catctcttta 660

tcctgccatc tacactttga actaccataa ggatggcttt gaatatatac ccggcaatca 720

cgccggaggt gcctacttcc acgagtactc cactctgcag tggttgactc tcgggaaact 780

aattatcaat gatcctttga aagtaaagaa accccttact cttactgtac agctaataga 840

aagtctaggg gccaaccatc ttttacttat cacccgaggg gatcttcgca cacccaaact 900

gcggacattc gctaaagata cccatgttct actaccacaa atattccatc ctaagggtat 960

gaatgcgaat aaaccattgt ccaaacgtag agctatgcag ttgtggctct acgccaaatc 1020

tgttaaagag gtgagcgaac gggatcttta tgctaaggtg aggcaattga tcccgacttc 1080

tgagttagac ctttttgacc ccgtggaggt gacccacttg gtcaattact tactattcat 1140

cagtcacctt tcctccgtat cttcatatga cgacatcttg tcgtctaaca tattccaaca 1200

tttcactatt ccaatcaaga acaagatacg ggaattggtc cagctattca ccggagcgga 1260

ccaatttaat caactgctca aagctctcga ctggcaaaca ttttcatact ctatgccagt 1320

agagaccatc catacacgtg cggccaatta tcaagtcgct aaaacgcttc gcatgtgcag 1380

agacctacca tgcgatgaat atgatcgtgt aaaggatgtc ctcagacagc tccctgacgg 1440

agtgacgttg tttgaggaaa acgacaagca agatccctca agccctgaag ccgaggactc 1500

tgaggatgac actgactccg tcgacttcaa ccttcctccc actcatgact tgcccccaaa 1560

ctttgatcct ctagacaagg gtaagtccat tatcgttgac actggcaacc cctcaacttc 1620

tacggcaccc gccgtcactt ttgctgccag tatcaactcc tcagcctcca caaatatttc 1680

atttggaagc ttcacacctg aggctgaggc cacccccctc cctcccatgg aaaagttgcc 1740

atgggatctt tggattcccc tactggaaca acatggcttt aaaggaaaga gcaaactgta 1800

caagcccacg ggtgaactaa tctgccccat aactgaaatc aaaacagttc cccactgccc 1860

ctttcccgat aaagtccagg atggctgtgt gcttgcgctt aaatctatta agcgtttcgc 1920

cacaaaaatg actatgctca gttctcgagc ttctgcctac acttctgaca ttaagaatag 1980

taggactggg aagctcttac ccgccatgaa catgccctgg aaagcctctc tcgcctatgt 2040

cactcaacat ggtgatagag aaattcctgg cgtcgtgatc catggtgccg gtggctgtgg 2100

caaatcttat gccatccaaa aatggttgag aagctgctct gatccttgtg cagctactgt 2160

ggtatgccca actctagaac tacgtaatga ctggctcaac aagatcggaa gctacgaaca 2220

aacgaatatc aaaactttcg agaaagcttt aattcagcca gtcaacaacg tagttatctt 2280

tgatgactac accaagctac cccctgggta catcgagacc atggtgtacc atcaccacaa 2340

cctcgacctg atcatactaa ctggggatcc tatgcagagt gcctaccatg aaactaacag 2400

ggatgcctat atctctttaa tacctgatgc ctctgccatc tttagtgagt actgcgagtt 2460

caacatcaac gccacccacc gtaatgtggc tgaactcgct tgcctcctcg gcgtctattc 2520

cgaacgccaa ggcaaactca ctgtttcctt cagcacagcc ccactttcta aaggtaaagt 2580

acccattttg gtaccctcta gaatgaagca agaggctttt gctgatgtcg gcaaccgttg 2640

catgacttat gccggttgcc agggtctcac tgcccctaaa atccaaattt tgatagacaa 2700

ccacactacg ttctgctctg aacaaacatt atacacttgt ctatcacggg ctgtggacca 2760

aatccacttc attaacactg gtcccaactc gcaggctttc tggactaaat tggaatcaac 2820

gccatatcta aaagctttcc tcgataatta ccgtgaggaa caaactgaac ggctaacatc 2880

tacagcccca gagcctgaag tccgtgagcc agcagcccca aaaacgcata tccctgtcga 2940

aaatacatct ggcttacgta tttctgcact tgacctgccg gaaaagcact ctcgcgagat 3000

ttttaaccag gcgcatggct tttccaatgc tattcaggga gatggcgtag ctcccatgtt 3060

ccagcatcaa caggcaaagg acgaaaccct tttcaaagct accatagacg ctagactctc 3120

tataacacat cctgatgaaa ataaaagaga gtttgccatg aagaaagaca ctggggacgt 3180

cctttttgtc aattacaaag ctataatgaa tctacctcat gagcctgttc cttttgaacc 3240

tcgtctctgg aacatctgca aagctgaagt gcagaacaca tacctagcca aacccattgc 3300

aaatcttatc aatggtacct taaggcaatc acccgatttt ccagccaata aaatagcctt 3360

attcctaaaa tcgcaatggg taaagaaaat tgagaaaatt ggagccatac cagttaaacc 3420

cggacagact attgcatcat ttatgcaaga gactgttatg ctgtatggca ccatggcacg 3480

ctacctccgt aagatgcggc aaagatatca acccgcacat atatttatca attgtgaaaa 3540

gactccggag gacttcaata aattcatact tgaacactgg tcacacaagc aagttgccca 3600

cactaacgat tttactgctt ttgaccagtc acaagatgct gccatgctcc aatttgaagt 3660

catcaaagct aggtacttca atatccccga ggatgttata gaaggataca tccaaattaa 3720

gctcactgct gaaatcttcc tcgggacact ttccatcatg cgcctatctg gtgaagggcc 3780

gaccttcgac gccaatactg aatgctctat tgcctacaac gccaccagat atcatattaa 3840

tgaagacgtc acccaagtat acgccggtga cgacatggct atggaccatg tgtgccctga 3900

gaagaagagc ttcaaagctt tggaaaagaa actgaaattg acctcaaaac ctctctatcc 3960

aaagcaaaaa cctggagact gggctgattt ctgcggctgg actataacgc cttatggcat 4020

cattaagaat cctaagaaac ttgatgcatg cctacaattg cacacccaac tgggcgatgc 4080

cgataaagtc gctaggtcat acgcactcga tgccaaatat gcttatgatc tgggcgatcg 4140

cattcatgag gttctgaatg ttgatgaaat gaccagccac ttcaatgtta taagacagtt 4200

gcacaaactg catcaacaag atgtgctggt cccacccgag actaccgtag ccacagcggt 4260

aaagtctcaa cctgacgtgg aggatctgtg gctccgtgcg cttagcttcc ctgactggac 4320

ggaccgcgcc caacttttaa aacagggtta attttgatgg agctggcgta cttagttaga 4380

ttactagagc acaataaatt cgagcgcacc aatctgcccc tttcctcacc cttagttgta 4440

cacggaatcg ctggcagcgg aaaatccacc atcctcacaa ctttccatca ccattacccg 4500

gctcacccta tcttctcaca cagtcccaca ttgcttgacc ctagcaaccg catataccaa 4560

cagtgcatca ctactgattc ggtgccagac ggtgctatcg tagacgagta caattacaaa 4620

gcactcgact actctcgctg tctagccctc ttcggtgatc cactccagct tccacattca 4680

cttcaacccc actaccactc tagtcgcacc caccgctacg gtcccaagct taccagccta 4740

ctcaacgacc tcttccacct ttctatcact tctcttgccc ccgtcgacag ccttgattac 4800

gctgatcctt ttgctgtcga cccctctgga tttaccattg ctgacgagga agtgtacaac 4860

tttgtctctc agcaggttcc cggcactttg ttgccgctcg atactgtagg tttagaatat 4920

tctagtgtta gcttttactg cagtgatctc cgacgctgtg tcgtactaag gccactcagc 4980

accttcatcg ccctcacccg cgccaagggc aacctcacca tcttcgattt caatgccagg 5040

tttagttcca ccacctgacc actccaagtc actcttcgtc cttgctattg gtataactgt 5100

ggtctctgca ttatttgtgc tgaagtccca cacttttccg attgcaggcg acaatattca 5160

ccgcttcccc tccggcggcc aatataaaga cggtactaag cagataaact actgtccacc 5220

tactcatgct aggtacccga aatatcctga ctacaagtgg cttgccgcca ccgccgccat 5280

cgtcatccct ctctgcctat atatttccta ccatcctggc aataatattc gccgtatttg 5340

cccttgttgc aatacatatc accacccctg agcccttctg taccatacac atagacgggg 5400

cgtctattac tatcactaac tgccccgatc ctgcagctat attaaataaa gtagccatag 5460

gcccctggcg agggttaagt taccacaata atttgaaata atcatgggag agctcactcc 5520

aactccagct gccacttact ccactgccga ccccacttct gcacccaagt tggccgacct 5580

agctgccatt aagtactcac ctgtcacctc ctccatcgcc acccccgaag aaatcaaggc 5640

cataacccaa ttgtgggtta acaaccttgg cctccccgcc gacacagtag gtaccgcggc 5700

cattgacctg gcccgcgcct acgctgacgt cggggcgtcc aagagtgcta ccctgctcgg 5760

tttctgccct acgaaacctg atgtccgtcg cgccgctctt gccgcgcaga tcttcgtggc 5820

caacgtcacc ccccgccagt tttgcgctta ctacgcgaaa gtggtgtgga atctgatgct 5880

ggccactaac gatccgcccg ccaactgggc caaggctggt ttccaggagg atactcggtt 5940

tgccgccttt gacttcttcg atgccgtcga ttccactgcc gcactggaac ctgctgaatg 6000

gcagcgccgc cctactgacc gtgaacgtgc tgcgcactcg atcgggaagt acggcgccct 6060

tgcccgtcag cgtatccaaa acggcaacct catcaccaac attgccgagg tcactaaggg 6120

ccatcttggc tccactaaca ctctctacgc tctgcctgca ccccctactg aataacgcca 6180

aacttaataa ggcgtgtggt tttctaaagt ttgtttccac tactggcata atatatttag 6240

ccagataaaa aaaaaaaaaa aaaaaaaaaa cccgggt 6277

<210> 25

<211> 8098

<212> DNA

<213> 5'端亚基因组的体外转录载体(Artificial Sequence)

<400> 25

tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cataatacga ctcactatag 420

ggagaggaaa accaaacctc acgtctactc cgctatccga gcgaaatagc agccccagca 480

taggcaatat cgcgctccat ggcccgagta cgtgataccc ttgacaggct ccgtgatccg 540

tcggtcctta cctcaattaa cgaggaagcc caccggcata tccgtcctgt ccttgcctcg 600

gcactcgtaa actgccctta cgcgttgacc gaggaagagg ccgactgtct cgaaaatctc 660

ggcgtgacag tcaatccctt cgccatccaa acgcatacac atgctgcagc taaaactgtt 720

gaaaatcgta tgttggaaat tgttggcacc caccttccta aagaaccctc caccttcata 780

ttccttaagc gaagcaagct tcgctaccta cgacgcgccg ccaacaacaa agacatattt 840

caaaatcaac acattgagcc taaagactta ttgcgttacg atgacgagtc ttgtggagca 900

atgccagaat gctccacaag cacagcctac atcagcgatg ctctccattt cttgagctac 960

gctcagcttg ggaaaatatt ccaggattct cccaagctga aaattctgct ggcaaccctg 1020

gtcctccccg tggaagccct ccataggcac ccatctcttt atcctgccat ctacaccttg 1080

aactaccata aggatggctt tgaatatata cccggcaatc acgccggagg tgcctacttc 1140

cacgagtact ccactctgca gtggttgact ctcgggaaac taattatcaa tgatcctttg 1200

aaagtaaaga aaccccttac tcttactgta cagctaatag aaagtctagg ggccaaccat 1260

cttttactta tcacccgagg ggatcttcgc acacccaaac tgcggacatt cgctaaagat 1320

acccatgttc tactaccaca aatattccat cctaagggta tgaatgcgaa taaaccattg 1380

tccaaacgta gagctatgca gttgtggctc tacgccaaat ctgttaaaga ggtgagcgaa 1440

cgggatcttt atgctaaggt gaggcaattg atcccgactt ctgagttaga cctttttgac 1500

cccgtggagg tgacccactt ggtcaattac ttactattca tcagtcacct ttcctccgta 1560

tcttcgtatg acgacatctt gtcgtctaac atattccaac atttcactat tccaatcaag 1620

aacaagatac gggaattggt ccagctattc accggagcgg accaatttaa tcaactgctc 1680

aaagctctcg actggcaaac attttcatac tctatgccag tagagaccat ccatacacgt 1740

gcggccaatt atcaagtcgc taaaacgctt cgcatgtgca gagacctacc atgcgatgaa 1800

tatgatcgtg taaaggatgt cctcagacag ctccctgacg gagtgacgtt gtttgaggaa 1860

aacgacaagc aagatccctc aagccctgaa gccgaggact ctgaggatga cactgactcc 1920

gtcgacttca accttcctcc cactcatgac ttacccccaa actttgatcc tctagacaag 1980

ggtaagtcca ttatcgttga cactggcaac ccctcaactt ctacggcacc cgccgtcact 2040

tttgctgccg gtatcaactc ctcagcctcc acaaatattt catttggaag cttcacacct 2100

gaggctgagg ccacccccct ccctcccatg gaaaagttac catgggatct ttggattccc 2160

ctactggaac aacatggctt taaaggaaag agcaagctgt acaagcccac gggtgaacta 2220

atctgcccca taactgaaat caaaacagtt ccccactgcc cctttcccga taaagtccag 2280

gatggctgtg tgcttgcgct taaatctatt aagcgtttcg ccacaaaaat gactatgctc 2340

agttctcgag cttctgccta cacttctgac attaagaata gtaggactgg gaagctccta 2400

cccgccatga acatgccctg gaaagcctct ctcgcctatg tcactcaaca tggtgataga 2460

gaaattcctg gcgtcgtgat ccatggtgcc ggtggctgtg gcaaatctta tgccatccaa 2520

aaatggttga gaagctgctc tgatccttgt gcagctactg tggtatgccc aactctagaa 2580

ctacgtaatg actggctcaa caagatcgga agctacgaac aaacgaatat caaaactttc 2640

gagaaagctt taattcagcc agtcaacaac gtagttatct ttgatgacta caccaagcta 2700

ccccctgggt acatcgagac catggtgtac catcaccaca acctcgacct gatcatacta 2760

actggggatc ctatgcagag tgcctaccat gaaactaaca gggatgccta tatctcttta 2820

atacctgatg cctctgccat ctttagtgag tactgtgagt tcaacatcaa cgccacccac 2880

cgtaatgtgg ctgaactcgc ttgcctcctc ggcgtctatt ccgaacgcca aggcaaactc 2940

actgtttcct tcagcacagc cccactttct aaaggtaaag tacccatttt ggtaccctct 3000

agaatgaagc aagaggcttt tgctgatgtc ggcaaccgtt gcatgactta tgccggttgc 3060

cagggtctca ctgcccctaa aatccaaatt ttgatagaca accacactac gttctgctct 3120

gaacaaacat tatacacttg tctatcacgg gctgtggacc aaatccactt cattaacact 3180

ggtcccaact cgcaggcttt ctggactaaa ttggaatcaa cgccatatct aaaagctttc 3240

ctcgataatt accgtgagga acaaactgaa cggctaacat ctacagcccc agagcctgaa 3300

gtccgtgagc cagcagcccc aaaaacgcat atccctgtcg aaaatacatc tggcttacgt 3360

atttctgcac ttgacctgcc ggaaaagcac tctcgcgaga tttttaacca ggcgcatggc 3420

ttttccaatg ctattcaggg agatggcgta gctcccatgt tccagcatca acaggcaaag 3480

gacgaaaccc ttttcaaggc taccatagac gctagactct ctataacaca tcctgatgaa 3540

aataaaagag agtttgccat gaagaaagac actggggacg tcctttttgt caattacaaa 3600

gctataatga atctacctca tgagcctgtt ccttttgaac ctcgtctctg gaacatctgc 3660

aaagctgaag tgcagaacac atacctagcc aaacccattg caaatcttat caatggtacc 3720

ttaaggcaat cacccgattt tccagccaat aaaatagcct tattcctaaa atcgcaatgg 3780

gtaaagaaaa ttgagaaaat tggagccata ccagttaaac ccggacagac tattgcatca 3840

tttatgcaag agactgttat gctgtatggc accatggcac gctacctccg taaaatgcgg 3900

caaagatatc aacccgcaca tatatttatc aattgtgaaa agactccgga ggacttcaat 3960

aaattcatac tcgaacactg gtcacacaag caagttgccc acactaacga ttttactgct 4020

tttgaccagt cacaagatgc tgccatgctc caatttgaag tcatcaaagc taggtacttc 4080

aatatccccg aggatgttat agaaggatac atccaaatta agctcactgc tgaaatcttc 4140

ctcgggacac tttccatcat gcgcctatct ggtgaagggc cgaccttcga cgctaatact 4200

gaatgctcta ttgcctacaa cgccaccaga tatcatatta atgaagacgt cacccaagta 4260

tacgccggtg acgacatggc tatggaccat gtgtgccctg agaagaagag cttcaaagct 4320

ttggaaaaga aactgaaatt gacctcaaaa cctctctatc caaagcaaaa acctggagac 4380

tgggctgatt tctgcggctg gactataacg ccctatggca tcattaagaa tcctaagaaa 4440

cttgatgcat gcctacaatt gcacacccaa ctgggcgatg ccaataaagt cgctaggtca 4500

tacgcactcg atgccaaata tgcttatgat ctgggcgatc gcattcatga ggttctgaat 4560

gttgatgaaa tgaccagcca cttcaatgtt ataagacagt tgcacaaact gcatcaacaa 4620

gatgtgctgg tcccacccga gactaccgta gccacagcgg taaagtctca acctgacgtg 4680

gaggatctgt ggctccgtgc gcttagcttc cctgactgga cggaccgcgc ccaactttta 4740

aaacagggtt aattttgatg gagctggcgt acttagttag attactagag cacaataaat 4800

tcgagcgcac caatctgccc ctttcctcac ccttagttgt acacggaatc gctggcagcg 4860

gaaaatccac catcctcaca actttccatc accattaccc ggctcaccct atcttctcac 4920

acagtcccac attgcttgac cctagcaacc gcatatacca acagtgcatc actactgatt 4980

cggtgccaga cggtgctatc gtagacgagt acaattacaa agcactcgac tactctcgct 5040

gtctagccct cttcggtgat ccactccagc ttccacattc acttcaaccc cactaccact 5100

ctagtcgcac ccaccgctac ggtcccaagc ttaccagcct actcaacgac ctcttccacc 5160

tttctatcac ttctcttgcc cccgtcgaca gccttgatta cgctgatcct tttgctgtcg 5220

acccctctgg atttaccatt gctgacgagg aagtgtacaa ctttgtctct cagcaggttc 5280

ccggcacttt gttgccgctc gatactgtag gtttagaata ttctagtgtt agcttttact 5340

gcagtgatct ccgacgctgt gtcgtactaa ggccactcag caccttcatc gccctcaccc 5400

gcgccaaggg caacctcacc atcttcgatt tcaatgccag gtttagttcc accacctgac 5460

cactccaagt cactcttcgt ccttgctatt ggtataactg tggtctctgc attatttgtg 5520

ctgaagtccc acacttttcc gattgcaggc gacaatattc accgcttccc ctccggcggc 5580

caatataaag acggtactaa gcagataaac tactgtccac ctactcatgc taggtacccg 5640

aaatatcctg actacaagtg gcttgccgcc accgccgcca tcgtcatccc tctctgccta 5700

tatatttcct accatcctgg caataatatt cgccgtattt gcccttgttg caatacatat 5760

caccacccct gagcccttct gtaccataca catagacgaa aaaaaaaaaa aaaaaaaaaa 5820

aacccgggtg ggcccgtcga ctgcagaggc ctgcatgcaa gcttggcgta atcatggtca 5880

tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 5940

agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 6000

cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 6060

caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 6120

tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 6180

cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 6240

aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 6300

gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 6360

agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 6420

cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 6480

cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 6540

ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 6600

gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 6660

tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 6720

acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 6780

tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 6840

attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 6900

gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 6960

ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 7020

taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 7080

ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 7140

ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 7200

gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 7260

ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 7320

gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 7380

tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 7440

atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 7500

gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 7560

tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 7620

atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 7680

agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 7740

ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 7800

tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 7860

aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 7920

tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 7980

aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 8040

accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc 8098

<210> 26

<211> 22

<212> DNA

<213> 扩增CymMV全长序列的正向引物(Artificial Sequence)

<400> 26

ggaaaaccaa acctcacgtc ta 22

<210> 27

<211> 30

<212> DNA

<213> 扩增CymMV全长序列的反向引物(Artificial Sequence)

<400> 27

ttttatctgg ctaaatatat tatgccagta 30

<210> 28

<211> 14

<212> DNA

<213> 针对全长及5’ 端亚基因组共有区域的正向引物(Artificial Sequence)

<400> 28

ccggaggact tcaa 14

<210> 29

<211> 15

<212> DNA

<213> 针对全长及5’ 端亚基因组共有区域的反向引物(Artificial Sequence)

<400> 29

ttgctagggt cgagc 15

<210> 30

<211> 18

<212> DNA

<213> 针对3’ 端CP区域的正向引物(Artificial Sequence)

<400> 30

tggcaataat attcgccg 18

<210> 31

<211> 30

<212> DNA

<213> 针对3’ 端CP区域的反向引物(Artificial Sequence)

<400> 31

ttttatctgg ctaaatatat tatgccagta 30

<210> 32

<211> 20

<212> DNA

<213> 针对本生烟Actin基因的正向引物(Artificial Sequence)

<400> 32

tggactctgg tgatggtgtc 20

<210> 33

<211> 20

<212> DNA

<213> 针对本生烟Actin基因的反向引物(Artificial Sequence)

<400> 33

cctccaatcc aaacactgta 20

Claims (10)

1.一种建兰花叶病毒株系，其特征在于，所述病毒株系的全长基因组序列如SEQ IDNO:1-10所示。

2.根据权利要求1所述的一种建兰花叶病毒株系，其特征在于，所述病毒株系的5'端亚基因组序列如SEQ ID NO:11-21所示。

3.一种体外转录载体，其特征在于，所述转录载体用于转录权利要求1所述的建兰花叶病毒株系的全长基因组序列和/或权利要求2所述的建兰花叶病毒株系的5'端亚基因组。

4.根据权利要求3所述的一种体外转录载体，其特征在于，所述转录载体的序列上游为T7启动子，序列下游包含SmaⅠ酶切位点。

5.根据权利要求3所述的一种体外转录载体，其特征在于，用于构建体外转录载体的引物组包括如SEQ ID NO:22所示的正向引物和如SEQ ID NO:23所示的反向引物；其中，所述正向引物引入T7启动子序列，所述反向引物引入PolyA位点及SmaⅠ平末端酶切位点；其中，所述病毒株系的全长基因组序列的体外转录载体序列如SEQ ID NO:24所示；所述病毒株系的5'端亚基因组序列的体外转录载体序列如SEQ ID NO:25所示。

6.一种引物组合物，其特征在于，所述引物组合物用于扩增权利要求1所述的建兰花叶病毒株系的全长基因组序列和/或扩增权利要求2所述的建兰花叶病毒株系的5'端亚基因组，其特征在于，所述引物组合物包括如SEQ ID NO:26所示的正向引物、如SEQ ID NO:27所示的反向引物以及Oligo(dT)引物。

7.一种采用如权利要求6所述的引物组合物扩增建兰花叶病毒全长序列及其5'端亚基因组序列的方法，其特征在于，包括如下步骤：

步骤1)RNA的提取及逆转录合成第一链cDNA；

对样本进行取样，采用液氮冷冻，提取RNA，利用引物Oligo(dT)反转录合成第一链cDNA；

步骤2)建兰花叶病毒序列的扩增；

以步骤1)的反转录产物为模板，PCR扩增建兰花叶病毒全长及其5'端亚基因组序列；其中，PCR扩增采用的引物包括如SEQ ID NO:26所示的正向引物、如SEQ ID NO:27所示的反向引物以及Oligo(dT)引物；

步骤3)建兰花叶病毒序列连接至载体并测序；

将步骤2)中PCR扩增获得的目的片段经胶回收后，用连接酶连接到载体，并取适量的连接反应产物转化大肠杆菌感受态，待克隆长出后，挑取单克隆进行菌落PCR验证，选取阳性单克隆进行测序，测序序列导入软件进行手动拼接，获得病毒序列；

其中，所述正向引物和反向引物扩增得到如SEQ ID NO:1-10所示的建兰花叶病毒全长基因组序列；所述正向引物和oligo(dT)引物扩增得到如SEQ ID NO:11-21所示的建兰花叶病毒的5'端亚基因组序列。

8.根据权利要求7所述的扩增方法，其特征在于，所述步骤2)的PCR扩增体系中采用高保真酶KOD-Plus-Neo；所述步骤2)的PCR扩增体系的扩增程序为：94℃变性2分钟；98℃10秒；55℃30秒，68℃4分30秒，30个循环；68℃7分钟。

9.一种如权利要求1所述的建兰花叶病毒株系的制备方法，其特征在于，包括如下步骤：

步骤A：体外转录载体的线性化；

权利要求3所述的体外转录载体经限制性内切酶SmaⅠ酶切后，进行琼脂糖凝胶电泳检测，确保载体被线性化完全；

步骤B：体外转录物的合成；

向步骤A中线性化完全的体外转录载体依次加入10×体外转录缓冲液、ATP、CTP、UTP、GTP、m7G(5')ppp(5')G、线性化模板、T7RNA聚合酶，37℃水浴2小时，获得转录出的单链RNA。

10.根据权利要求9所述的建兰花叶病毒株系的制备方法，其特征在于，还包括体外转录物的接种及侵染烟草验证步骤；

其中，所述体外转录物的接种包括步骤：将转录出的RNA与等体积2×GKP缓冲液混合，摩擦接种3-4周龄的本生烟叶片；

其中，所述侵染烟草验证包括步骤：提取接种病毒的本生烟的总RNA，反转录获得cDNA第一链，针对CymMV全长及5'端亚基因组共有区域设计如SEQ ID NO:28和SEQ ID NO:29所示序列的引物组及针对CymMV 3'端CP区域设计如SEQ ID NO:30-SEQ ID NO:31所示序列的引物组，分别进行PCR扩增，验证体外转录物的侵染活性。