CN109825567B - 肺炎诊断用circRNA生物标志物及应用、引物和试剂 - Google Patents

Abstract

本发明涉及生物技术领域，特别涉及肺炎诊断用circRNA生物标志物及应用、引物和试剂。该生物标志物选自hsa_circ_0018429、hsa_circ_0125357、hsa_circ_0026579、hsa_circ_0099188中的一种或几种。利用本发明的circRNA中一种或几种，在全血中的表达水平能够很好的筛选肺炎感染患者；当四种标志物组合使用时，AUC可以达到0.8776，灵敏度和特异度分别可达到89％和71％，说明对肺炎感染具有较好的诊断效果，显示出了其作为生物标志物的优势。

Description

肺炎诊断用circRNA生物标志物及应用、引物和试剂

技术领域

本发明涉及生物技术领域，特别涉及肺炎诊断用circRNA生物标志物及应用、引物和试剂。

背景技术

肺炎按医院内外发病可分为社区获得性肺炎(CAP)和医院获得性肺炎(HAP)。近年肺炎其发病率和病死率都有升高趋势。肺炎病原体较复杂，各种感染和非感染之间，在临床表现、胸部X线等常有交叉重叠，难以鉴别。肺炎常见病原体有细菌、病毒、支原体、衣原体、立克次体、螺旋体、真菌、放线菌和寄生虫。但一般情况下病原体较难确定。

对肺炎的诊断主要依靠临床表现、实验室检查及胸部影像学检查来诊断。新型的分子标志物近年来也有很多进展，包括对肺炎病人代谢组学或者对肺炎病人miRNA组学的研究等。但这些研究都会存在一些问题，例如检测方法较复杂、标志物不稳定易降解等。

非编码RNA(ncRNA)，包括短microRNA(miRNA)、长非编码RNA(lncRNA)和最近描述的环状RNA(circRNA)，共占真核转录组总RNA的95％左右。circRNA具有独特的环状结构，稳定性强，是作为疾病的分子标志物的理想候选者。已报道circRNA可作为许多疾病的有效诊断和生物标志物，但目前仍然没有使用circRNA这种易于监测和高度稳定的分子靶标用于诊断肺炎，尤其是社区获得性肺炎这种常见且高度致命的疾病的报道。探索circRNA的表达与在肺炎患者的关系对了解肺炎感染的诊断具有重大意义。

发明内容

有鉴于此，本发明提供了肺炎诊断用circRNA生物标志物及应用、引物和试剂。该生物标志物选自hsa_circ_0018429、hsa_circ_0125357、hsa_circ_0026579、hsa_circ_0099188中的一种或几种。本发明具有的技术效果为：

利用本发明的circRNA：hsa_circ_0018429、hsa_circ_0125357、hsa_circ_0026579和hsa_circ_0099188中一种或几种，在全血中的表达水平能够很好的筛选肺炎感染患者；当四种标志物组合使用时，AUC可以达到0.8776，灵敏度和特异度分别可达到89％和71％，说明对肺炎感染具有较好的诊断效果，显示出了其作为生物标志物的优势。

本发明还提供一种用于肺炎感染检测的引物组合物，包括本发明所述用于检测circRNA的正向引物和反向引物。本发明所述的circRNA组合物具有在制备肺炎感染检测相关产品中应用的能力。

为了实现上述发明目的，本发明提供以下技术方案：

本发明提供了肺炎诊断用circRNA生物标志物，选自如下生物标志物中的一种或几种：

(1)hsa_circ_0018429：序列如SEQ ID NO：1所示；

(2)hsa_circ_0125357：序列如SEQ ID NO：2所示；

(3)hsa_circ_0026579：序列如SEQ ID NO：3所示；

(4)hsa_circ_0099188：序列如SEQ ID NO：4所示。

hsa_circ_0018429位于第10号染色体上，全长19579个碱基。

hsa_circ_0125357位于第4号染色体上，全长13253个碱基。

hsa_circ_0026579位于第12号染色体上，全长24620个碱基。

hsa_circ_0099188位于第12号染色体上，全长341个碱基。

本发明还提供了该circRNA生物标志物在制备肺炎诊断试剂中的应用。

优选地，肺炎为社区获得性肺炎。

本发明还提供了肺炎诊断用circRNA引物，选自如下引物组中的一种或几种：

(1)用于扩增hsa_circ_0018429的引物组；

(2)用于扩增hsa_circ_0125357的引物组；

(3)用于扩增hsa_circ_0026579的引物组；

(4)用于扩增hsa_circ_0099188的引物组。

作为优选，用于扩增hsa_circ_0018429的引物组由SEQ ID NO：5所示正向引物和SEQ ID NO：6所示反向引物组成。

作为优选，用于扩增hsa_circ_0125357的引物组由SEQ ID NO：7所示正向引物和SEQ ID NO：8所示反向引物组成。

作为优选，用于扩增hsa_circ_0026579的引物组由SEQ ID NO：9所示正向引物和SEQ ID NO：10所示反向引物组成。

作为优选，用于扩增hsa_circ_0099188的引物组由SEQ ID NO：11所示正向引物和SEQ ID NO：12所示反向引物组成。

具体序列如下：

引物名称	序列5'——3'
		hsa_circ_0018429-F1	GATCAGGATCTGAAGGAGCTGGG
hsa_circ_0018429-R1	ACAGGAAACCTTGGAGGGAC
		hsa_circ_0125357-F1	CATGAGTGTCAGCTGGAGCG
hsa_circ_0125357-R1	AGACAAAGCTCCTTTAATTAGCTGC
		hsa_circ_0026579-F1	TAAACCCTGGAGTTCCTGTCCAA
hsa_circ_0026579-R1	CCAGATGCCTAGGGCAAGC
		hsa_circ_0099188-F2	GCCCAACCTAGAGGAAGTTGT
hsa_circ_0099188-R2	CCATACCTGATGCCACCCTT

本发明还提供了肺炎诊断试剂，包括上述肺炎诊断用circRNA引物。

作为优选，还包括用于PCR扩增的其它试剂。

作为优选，用于PCR扩增的其它试剂为彩色荧光定量预混试剂。

在本发明中，肺炎诊断的方法为检测circRNA在肺炎感染患者和健康人群的全血样本中的表达水平。

上述应用中，表达水平可为绝对表达水平或相对表达水平。所述相对表达水平可为相对于β-ACTIN的相对表达水平。所述表达水平可为circRNA组合在全血中的表达水平。

本发明肺炎诊断用circRNA生物标志物的筛选方法如下：

1)建立统一标准的标本库和数据库：从医院采集血样样本，同时系统收集患者的病理学资料和临床资料；

2)环状RNA差异表达谱分析：选择肺炎感染病人和健康个体的全血，提取RNA，使用博奥晶典人类环状RNA v2芯片筛选技术，并通过差异分析，得到在肺炎感染病人和健康个体的差异表达的circRNA；

3)用实时荧光定量PCR方法筛选出可用作检测生物标志物的血清circRNA：对已筛选的血清差异表达的circRNA在大样本人群中使用上述引物组合进行定量分析验证，确定能够用于区分正常人和肺炎感染病人的circRNA：hsa_circ_0018429(SEQ ID No.1)，hsa_circ_0125357(SEQ ID No.2)，hsa_circ_0026579(SEQ ID No.3)，hsa_circ_0099188(SEQID No.4)。

本发明使用的检测方法可以选自：RT-PCR方法、芯片技术、测序技术、荧光定量PCR方法等中的至少一种。

本发明提供了肺炎诊断用circRNA生物标志物及应用、引物和试剂。该生物标志物选自hsa_circ_0018429、hsa_circ_0125357、hsa_circ_0026579、hsa_circ_0099188中的一种或几种。本发明具有的技术效果为：

利用本发明的circRNA：hsa_circ_0018429、hsa_circ_0125357、hsa_circ_0026579和hsa_circ_0099188中一种或几种，在全血中的表达水平能够很好的筛选肺炎感染患者；当四种标志物组合使用时，AUC可以达到0.8776，灵敏度和特异度分别可达到89％和71％，说明对肺炎感染具有较好的诊断效果，显示出了其作为生物标志物的优势。

本发明还提供一种用于肺炎感染检测的引物组合物，包括本发明所述用于检测circRNA的正向引物和反向引物。本发明所述的circRNA组合物具有在制备肺炎感染检测相关产品中应用的能力。

附图说明

图1为肺炎患者全血的circRNA芯片表达谱聚类分析；

图2为hsa_circ_0018429、hsa_circ_0026579、hsa_circ_0125357、hsa_circ_0099188在肺炎患者和健康对照人群表达对比；

图3为hsa_circ_0018429、hsa_circ_0026579、hsa_circ_0125357、hsa_circ_0099188的ROC分析图。

具体实施方式

本发明公开了肺炎诊断用circRNA生物标志物及应用、引物和试剂，本领域技术人员可以借鉴本文内容，适当改进工艺参数实现。特别需要指出的是，所有类似的替换和改动对本领域技术人员来说是显而易见的，它们都被视为包括在本发明。本发明的方法及应用已经通过较佳实施例进行了描述，相关人员明显能在不脱离本发明内容、精神和范围内对本文所述的方法和应用进行改动或适当变更与组合，来实现和应用本发明技术。

本发明提供一种用于检测肺炎感染的circRNA组合物及其及其制备方法与应用。本发明全血circRNA组合物选自下列中的至少一种circRNA：hsa_circ_0018429，hsa_circ_0026579，hsa_circ_0125357，hsa_circ_0099188。本发明还提供一种用于肺炎感染检测的引物组合物，包括本发明所述用于检测circRNA的正向引物和反向引物。本发明所述的circRNA组合物具有在制备肺炎感染检测相关产品中应用的能力。

本发明提供的肺炎诊断用circRNA生物标志物及应用、引物和试剂中所用试剂或仪器均可由市场购得。

下面结合实施例，进一步阐述本发明：

实施例1CAP病人与健康全血对照人群全血的circRNA表达分析

一、对象和方法

1.对象

对象来自2015年1月至2016年12月期间北京大学人民医院肺病科收集的样本，最终选取了6对社区获得性肺炎感染和健康人群的样本，样本来源均排除自身免疫性疾病和炎症病性疾病患者，年龄与性别比例与研究对象相似。

2.方法

(1)样本采集：入院后2天内，在无菌的预凝血管中收集外周静脉血样并立即离心；将得到的血清样品保存在-80℃直至分析。

(2)全血总RNA提取：根据产品说明书，用TRIzolTMLS试剂(Invitrogen，USA)提取总RNA。将提取的RNA溶解在不含RNase的水中，并使用Nanodrop 2000(Thermo Scientific，USA)定量。通过1％凝胶电泳确定RNA的完整性。

(3)对样品进行circRNA表达谱分析：

博奥晶典人类环状RNA芯片v2表达谱芯片检测以待测样品的totalRNA为起始，进行体外扩增和荧光标记，过程采用Ambion WT Expression试剂盒。

反转录合成First strand cDNA：以TotalRNA为起始，含有T7启动子序列的Random引物，使用First Strand Enzyme Mix合成First strand cDNA。合成Second strand DNA：用Second Strand Enzyme Mix将DNA-RNA杂合体中的RNA链转化为Second Strand cDNA，合成双链DNA。

体外转录合成cRNA：以Second Strand cDNA为模板，用T7Enzyme Mix合成cRNA。

cRNA纯化：使用RNA纯化柱纯化cRNA，除去反应中的盐、酶等试剂，并对cRNA进行定量、质控。

反转录：以cRNA为模板，RandomPrimer为引物，CbcScriptⅡ酶进行反转录。纯化回收反转录得到的cDNA并定量。

标记：以反转录的cDNA产物为模板，RandomPrimer为引物，用Klenow Fragment酶合成cDNA互补链并掺入带有荧光基团的dNTP(Cy3-dCTP)，纯化并定量标记产物。带有荧光基团DNA即可用于芯片杂交。

博奥晶典人类环状RNA芯片v2杂交扫描后的tiff格式图片数据采用FeatureExtraction提数软件进行预处理分析，然后采用GeneSpring GX软件计算基因表达差异和统计学显著性p值。数据处理过程主要包括：芯片扫描tiff图用FeatureExtraction软件处理得到原始数据文件(.txt)。将原始数据文件(.txt)导入GeneSpring软件，写入分组等参数信息。进行每个样品的数据归一化和QC分析。用Cluster3.0软件进行Cluster分析和图形化展示。根据分组信息，进行差异比较，得到差异基因。

3.结果

关于CAP患者全血的circRNA芯片表达谱聚类分析见图1。最终得到8296个明显具有表达变化的差异circRNA，包括有3904个上调circRNA，4392个下调circRNA，并且发现这些异常表达的circRNA能够明显的将病人和健康人群进行分类。鉴于其潜在的诊断价值，本申请通过以下实例采用大规模样本进行筛选异常高表达的单个或circRNA分子标志物组合。

实施例2实时荧光定量PCR检测CAP患者全血中的circRNA分子标志物的表达

一、对象与方法

1.对象

对象来自2015年1月至2016年12月期间北京大学人民医院肺病科收集的样本，最终选取了30对肺炎感染和健康人群的样本，样本来源均排除自身免疫性疾病和炎症病性疾病患者，年龄与性别比例与研究对象相似。

2.方法

(1)样本采集：入院后2天内，在无菌的预凝血管中收集外周静脉血样并立即离心；将得到的血清样品保存在-80℃直至分析。

(2)全血总RNA提取：根据产品说明书，用TRIzolTMLS试剂(Invitrogen，USA)提取总RNA。将提取的RNA溶解在不含RNase的水中，并使用Nanodrop 2000(Thermo Scientific，USA)定量。通过1％凝胶电泳确定RNA的完整性。基于A260/A280值评估的RNA样品的质量；OD值在1.8和2.0之间的样品，浓度>90ng/μL，被认为适合进一步分析。

(3)定量PCR：根据产品说明书使用PrimeScript RT试剂盒(Takara Bio，Japan)进行定量RNA的逆转录。简言之，将630ng RNA(Nanodrop 2000)和4μL PrimeScript RTMaster Mix(Takara Bio，Japan)与不含RNase的水混合至终体积20μL。然后，将混合物在37℃的水浴中温育15分钟以完成逆转录并获得总cDNA。定量PCR用1.6μL的cDNA，5μL的SYBRgreen预混实施例的Taq(Takara Bio公司制，日本)，3μL无RNA酶的水，和0.4μM引物(reverse和forward)执行，筛选出可作为分子标志物的circRNA所用引物如下表：

表1可作为分子标志物的circRNA所用引物

使用ABI PRISM7500序列检测系统(Applied Biosystems公司，LifeTechnologies，USA)测量，在95℃下保持30s预变性，之后在95℃下变性5s，60℃退火30s，延伸时间根据碱基长短决定，设定循环数为50。将circRNA的表达水平用作稳定的内源对照(β-ACTIN)用于标准化。使用2^ΔCT方法定量circRNA的相对表达水平。

3.结果

根据倍数变化值选择排名前100位的上调的circRNA并且p<0.05。使用qPCR检查这些circRNA在包含30名CAP患者和30名对照受试者的中的差异表达。以在80％以上的CAP患者外周血样本中明显上调的表达作为选择标准，本申请鉴定了4种circRNA用于测试作为潜在的生物标志物，分别是hsa_circ_0018429，hsa_circ_0026579，hsa_circ_0125357，hsa_circ_0099188，CAP患者比健康对照人群的表达明显增高，且具有统计学意义(P<0.05)(图2)。为了评估全血中hsa_circ_0018429，hsa_circ_0026579，hsa_circ_0125357，hsa_circ_0099188的诊断价值，用ROC分析了4个circRNA单独作为标志物和作为一个组合对CAP的诊断能力，见下表。

表2 4个circRNA单独作为标志物和作为一个组合对CAP的诊断能力

之后绘制了CAP区别于健康对照人群的ROC曲线，见图3。曲线下面积(AUC)显示，全血hsa_circ_0018429，hsa_circ_0026579，hsa_circ_0125357，hsa_circ_0099188都具有较好的诊断价值，hsa_circ_0018429的AUC为0.8216，hsa_circ_0026579的AUC为0.7733，hsa_circ_0125357的AUC为0.7730，hsa_circ_0099188的AUC为0.6978，将hsa_circ_0018429，hsa_circ_0026579，hsa_circ_0125357，hsa_circ_0099188作为一个组合用于区分CAP和健康对照人群时，发现其AUC达到0.8776，说明hsa_circ_0018429，hsa_circ_0026579，hsa_circ_0125357，hsa_circ_0099188能够作为CAP诊断的分子标志物，且作为组合时，效果更好。

对比例1

在研发过程中，采用多组引物进行PCR扩增，但只有实施例2表1的引物组特异性强。现将其中一组对比引物的序列及对比试验结果呈现如下：

表3circRNA所用对比引物

引物名称	序列5'——3'
		hsa_circ_0018429-F-对照	TCAGACATTCCTCACTCTCACAGA
hsa_circ_0018429-R-对照	TCAAGTCACCAGCATTCCCA
		hsa_circ_0125357-F-对照	ACCTTTTGCAAGCTCACAGCA
hsa_circ_0125357-R-对照	AGACAAAGCTCCTTTAATTAGCTGC
		hsa_circ_0026579-F-对照	ATTTAAACCCTGGAGTTCCTGTCC
hsa_circ_0026579-R-对照	TCCTCTCAGCATCAGATCGGC
		hsa_circ_0099188-F-对照	TTGTGTGGCAAGAGGTGGGAA
hsa_circ_0099188-R-对照	CCTCTTCCATGAAGTCTCGCC

表4引物扩增速度对比

注：Ct值表示扩增量达到阀值时的循环数，本实验每种引物做3个重复，取均值。Ct值越小表示其扩增速度越高。“--”表示没有检测到循环数，即没有特异性产物被扩增。

结果显示，实施例2的引物组与上述对照引物及其他引物组进行对比发现，在相同的荧光定量PCR实验条件下，实施例2的引物组能够对circRNA进行特异性扩增，且扩增速度高于对比引物组。

以上所述仅是本发明的优选实施方式，应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。

序列表

<110> 博奥生物集团有限公司

北京大学人民医院

<120> 肺炎诊断用circRNA生物标志物及应用、引物和试剂

<130> MP1832071

<160> 12

<170> SIPOSequenceListing 1.0

<210> 1

<211> 19579

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 1

gttgtaatga tgcttttgtt gaagtaggca tgcctcgaag tccttcccat tctgggaatg 60

ctggtgactt gaaacagatg atgtgtccct ccaaggtttc ctgtgccaaa aggcagacag 120

tggaactatt gcaaggcacg aaaaactcac acttacagta tgtattttaa tctttaaaga 180

gcccttgata ttttgaaatg atatatattt gttctcaact ctatagttag tactctttga 240

ttcaagtcat gtttgatctc atccatgggt gttttcattt taactttttt aaaaaaaata 300

ttaaggaata atatctgtta attttcaccc ttctctgtct tctctatagt cccttcccca 360

tttaatgaat tgatttatat ctgtgtcctc taaaccgtct gtattactct gaaactgtag 420

cattctctct gctcttcagt aatcagcagg gccattttta ttctctgatt tttgctgtta 480

gaaattttcc cactgtgaat tctccttcat ttggctttga atttctttct tactgtggct 540

ctcttttttc ccaaggtagt taattgcaaa cttttaaata aatgcttgac attatcatga 600

ctttgtttat tccaaatcac tttggttata aatacataag ttgtattaaa tattaaatat 660

aactcttcct taccatagca cagttaaagt atgtcatcct tactcttttt ttttttcctc 720

catgtatttt ggacagtctc tacaatgtaa ggttttgttc ccacctattc cgtccacctg 780

atttcctctg ctgtcttatt agttcctggt atacccttct agagtgtctt tatgcaaaga 840

ttagcgtaca tggttttatt tcctcccatt cttattaaaa tccattttcc tttataaact 900

ctttggtcaa tcattattac ccaccgttgt ccagttctcc tttatctgtg tttttcttta 960

catggagcta atacccaaaa ttaatgttct cattataggc tagagaaaca gttttacttc 1020

cttgaaacta ttgaagcatt taaaatagat acgtattctt gtcatcactg agtatgtcct 1080

gaaccttctt atcatcatct tttggcttaa gctgcagcct ccagaaaggt ctccctgcct 1140

ccagtctttt attagcttga tctatccttt attttgcttc agcaatttac tttcgaaaac 1200

aggtctaatc ttgtcacact ctggcttcaa atcaccaatg gtctgtgttg cctgcttcct 1260

ttcctttact tatgctcttc cctctgagtt gctgttctcc tgacctttgg caccagacaa 1320

cttgcagatg ctcttaagtt cccaggtcaa atgtcctttg tgtagcctct gtcaactttt 1380

gtcaagcaaa gttcattgtc cctctttttg tattccccaa gtacttttct aaattcctct 1440

aaattccctt ggcacagcag ctgagtagtt gaacaaataa aatcatgaat gaataaacaa 1500

aacagtactt gattgatatt gcaatcccta ttttgagcct aaggtgcagt tagatttgaa 1560

cttgtggtgc atgcattctg tacatatcat gtgttgtaat tcacatattt gctgctctaa 1620

gaagcacttt tacgctctgc ctgtccccct caacagactt tgaagaatat ttcttattta 1680

tctgctacat tttatttgtt accatcatat acatatttca tacacacgaa ttggatcatc 1740

tacatccctt caaattgact ggatctctgt ttggactttg catggatgaa tcattgatat 1800

ctccttgatg tataaaaatt agaaatctca tctgtacatc tattccacct atatcatatg 1860

cagtatacca tatatactat atatactgtg tttgaatgtg catcatgaaa tagtttcaag 1920

ctttagccct acagtcagag tgcccaattt caaattccat ctctaccatt cacattagag 1980

gtatgacctt ggacaatata cttccttcat ccacaaagtg gggataacag tagtacctgt 2040

gtcatagcat tgttgtgagg attaaatgag agactacgtg taaatagctg atgatgataa 2100

acatgcatag taaatgtggc atgtaggaaa cattcagtac atttgtatat atagtggagt 2160

gttaaattcc acactcttat ttcacagcag cactgacagg ttgctctcag accctgaact 2220

gagtgctacc gaaagccctt tggctgacaa gaaggctcca gggagtgagc gcgctgcaga 2280

gagggcagca gctgcccagc aaaactccga aagggcccac cttgctccac ggtcatcata 2340

tgtcaacatg caggtaatgg taataaaata ggaaagccac tcaaatgtca tatgtttggg 2400

ttctggtatg gagaattcag tgagtgttca gcagtagtgc agaaattagg ttttccttct 2460

gtgctacatt caaaatgaaa tttaaatata tatgtagcag ttagtttgct ataaacatta 2520

aaaatgaaaa gcagtattta ccttaagtga gaattcttta aaatatatta aaaatggtgc 2580

agaatggtct gtgtcactgt cttatgcaaa taacattatt catgtgttac ttaaaaaatt 2640

ttcagatgag tttgcgggac tgcatttcta agtattcttg cttcatgaca tagtcttgga 2700

taaatagata tactattttc tatgtaatga agatgatgaa tgtgtttggt gccatttaaa 2760

gatagttatg agaataacga atgtggagtg ttctttgaaa ctagatgatg aatattattg 2820

atgattgtgt tggttcacag attttttatg attataaaaa caacttcctt taaataattt 2880

ttatgattaa acaacttcct caataggaac tttctgtgtt tccattttca aactattagt 2940

ctaattactc agaatagact attgaccatg aaaatgcaga aattatagat gacaatatga 3000

aagataagga tttgtgcttt gtgcttttag agatgcctac aagcatttga tgtcagacca 3060

gaattgctga gtagacttac ctgttttatc tttcaccacc aaaaaagagg aagaacgttc 3120

cacaaaatcc tagaaacaga aaagctgtca tagatgtgtc aaagaaatta attcatggag 3180

ctaaactata ttactttaat tccagtgcaa tctgcagttt agtcctcctt ctctattttt 3240

ttaatccttt gcattggaac attttcctaa gtttttcgat gaattttttt ttaaataaaa 3300

gctatggcct ttataatgtg tcagtgttta aaaaagacat ttaaggccgg gcacagtggc 3360

tcacacctat attctcagca ctttcagagg ccgaggcagg tggattgctt gagtcctgga 3420

gtttgagacc agcctggaca acgtggcaaa accccatctc tacagaataa ttctaaaaaa 3480

ttagcagggc gtggtggcat gcacctgtag tcccagctac ttggaggccg aggtgggagg 3540

atcacctgag cctgggaggc agaggttgct gtgagccaag attgcaccac tgcactctag 3600

cctgggagag agagcgagac cctgtctcaa aaattaaaaa taaataaaat aaaaaagaca 3660

tttattacat tttctctggt ggatgtattt tcagttaata tgaattacaa tttatttaac 3720

aaatatcaaa tcccctttat aatcttttga tggtttttgt cttgtggttc ttgtcacctt 3780

ttccatcaga tttatacctg cagtgaacca gtttcttgtt tctttctttt aggatggatg 3840

agcatgttct taatgtttga gtttcagcct aactcgatgt atttatgttg ttagtgaaca 3900

gttgactctt aataaatcac taagaaatca atgataatat tcactgagca ttgacaggga 3960

gaaagattga aaacttggcc tgatttcctt ggctgaaaat gatctgtatc tcctgattgc 4020

tcttggagtg ctatatcccc agctctcttt ttatgcttat ctcagtttct aatttatttt 4080

atagttattt ctttttccca accctcctaa gactgcaagt cagccaggca tggtggctca 4140

cacctgtaat cccagaactt tgggaagcca gggtgggcag atcacctgag gtcaggagtt 4200

cgagaccagc ctggccaaca tggcgaaacc ctgtcactac taaaaataca aaaattagcc 4260

cagcttggtg gtgggcgcct gtagtctcag ctacttggga ggccgagata gaagaatcac 4320

ttgaacccaa gaggtggagg ttgcagtgac ctgagattgc accactgcac tccagcctgg 4380

gcaacagagc gagactctgt ctaaaaaaca aacaaacaaa caaacaaaaa aactgcaagt 4440

catcgaggct gtagcttatt tttgattagt tgttgtattc ttccacaaag ctgagcatag 4500

tctctttaac cagtaggccc tcaatgaata tttgttgaac aagtaaattt aggttctccg 4560

tataccttgc ctttaggcat taataattaa aaattgattt taactcccaa tgccatttta 4620

gtcctaggat ttaaaaactt catcaaaaat attatctaaa aatctggctt tcctgacaca 4680

tggctatgtt tttagttatc acatgtcctt gtttgtctgg gacagcctct atttactttt 4740

attatcttgg cctaattgtt attcacagca catttcacct tcagaaatgt cctgtttcag 4800

accacagatt atataggtac cttatatatg atggtatagt ctttctggtt aaagagctct 4860

tgccaattgg cccttggtcc ttgtggtagg cctttgagac atgcttagat caagtcttag 4920

ctatttccta aattggggag ttgataacac tcagaaaaga tttgaaatgt agctgtatct 4980

gcactacttt cttcaaaatg atctatctta gtcctcacat acacttgact acatcgtagt 5040

tactcattca gttttaatgg aatgaatcaa tggtgctatc ctagccctgt gcaataaaca 5100

gtaactaggt ttaattttat ggcattgctt aatgtcatta taaattaaag ttagacatct 5160

gccatttatc tcacatctca cttcaaaagc aaagactatg ccagttaggt taaataattt 5220

cttaaggtat cttgacatca taccacttca ggccacgctt acctaaatga agaggggatt 5280

tttgagaccc taagggggcc aatagataca tgacagcatt ttggaaatgt atactttcaa 5340

aacccacatt attattatta ggctgttcac tatattctag cagacattgg ctctggtgtg 5400

ctaggtattt atggtaaata agattttttt ttcttttttt aaggaaaaat tctaactctt 5460

aaagtcaacc ttaaagtcag aagtggtgat ttattattac taattaatta gtgttggaaa 5520

catgtttaaa cttacagaca taccttggat ttctgtggca ttattgtgca aagcttggtg 5580

tatgtctcag gcagattcat ggaacacagt caaaaccttt gttctctaac catggagtgt 5640

tggcatgaca tttggagaga ctgtcaataa aggtattttc tgtttcattt tcaggcattt 5700

gactatgaac agaagaagct attagccacc aaaggtatgt aatacactaa taacttacac 5760

ttgactatat tttagtacac tcataaatgt tttttgagta ctcatggtga atcgagaact 5820

tgaaaactca gaaaaagaaa cctagaatat ttgggttcaa gttgcataca ttctgttgaa 5880

tatattttgt tgaatggttt agcattagac caattaagaa atcatttagc attttttcat 5940

ttatcattac ttggataaaa tatttcatta agatattttt agacattttg tgttaagaaa 6000

taacattcag tgataatttc ttcacatata tcaaagaaac gacacttatc atcagtattt 6060

tattgaagaa atgcattaaa cataccaagc attggtttta ttttgtttcc aaagctatgt 6120

taaagaaacc agtggtgacg gaggtcagaa cgcccacaaa tacctggagt ggcctgggtt 6180

tttctaaatc catgccagct gaaactatca aggagttgag aagggccaat catgtgtcct 6240

ataagcccac aatgacaacc acttatgagg tttgtagagt catgtcctac tcattcttcc 6300

tgtctgttct ttcagcaaaa gaggacagtc cttcccccac cctcattctt tatgtaccta 6360

agggaaaatg attagtggtg acttgttttt atttctattc ttgtaaacac actgttataa 6420

aactgatgca ctcttgtgta ttatgatctt ttatctgtat tctctcaaag ttgttggtca 6480

gctcttaaag agatgtaaat ttggatgtga gtttttgttg agtgtgggat gtgtcataaa 6540

tgataagcgt agggctgaga ttaagcaaaa gggagatgta atatttcata aaaatgtcta 6600

aagagtccta ttatccaagg gtttcactag caaggtcacg atgggggaaa aggcaaatat 6660

tcaacaaaaa taggttttca gatatgagtt aaaaattcag tgtgctcaga tgtgccaaga 6720

acacagcaag aagtatctga aacaagtgct ccaaaatttt tatgtttccc agatgttggc 6780

tgccctgctc agggtgagac tgggaagctg gcattcctac catatggaag ggatggagag 6840

ttccatactc cacagccagg ctacacgcga ttcaccaagc aggccttttc catttcaaag 6900

tatcagattt tggctctaag cctttgggag tcggggtggg tctagagaga gcgtgcacac 6960

atacacaatg ctgggagact gtacacaccc aaacatggat gaaaatgatt aatccaggag 7020

tcacagtgtt caaccagaga gagcactgta ggccaaaaaa caaacactgg ttaagggttt 7080

cataattgtt agtctaataa ctagcatttg tagattgacc ttttctccac ccatttgctt 7140

tgtaataaat taggctattg atgaaacaca aaagcaaaca tgctgctttt tttttttttg 7200

tctcttttga gtaagtgaaa aagaaaatac tattgaaata ctaaaagagt ctcctgtgaa 7260

tttcattttg ggagttatag tagcaacatg gcaggctgtg ttttaaagat tgaagttacc 7320

ttcagcagca gaatgtagga accattatga aaaatgttga ggctctagga ggtacactct 7380

tctttcttcc attataacac agagagaaaa taaacatctc cttttgttaa cagagaccat 7440

aaaactgctg tgctcattct tagagtttca cctgatgtac ggtatggcta ttcctttcag 7500

cccatccttg cttttgacgg agtgaagtgt gccagattgt tgactgtgtt cctatagttt 7560

caaagatgaa gtatttgctt tagaaatgct cattcagttc tgctttctaa gaccaacagt 7620

aggacaagtt gaaaatgatg ttcatttcgg taatggagtg gtcaagttca gggttctgat 7680

aaccacctct gaccagatgt atagctttgg tgagggactt cactcatctg agctgtgtct 7740

cattcaggtt gtgctgagaa gggcattgga gagggcatcc agtgattagc atgatgtgtt 7800

gtcagttctt agtgatattt aaaaaaaatt tttttttttt tttgacgtgg agtctcactc 7860

aaccccccta agctgcagtg cagtggcacg atctcggctc acggcaacca ccatctcctg 7920

ggttcaggca attctcccat ctcagcctcc cgagtagctg ggattacaga cacccaccat 7980

catgcccagc taatttttgt atttgagtag agacagagtt tcaccatgtt gaccaggctg 8040

gtcttgagct cctgacctca ggtgatctgc ccacctcgac ctcccaaagt gctaggatta 8100

caggtgtgag ccgccatgcc tggcctagtg atattttaaa aaattttact ttattaagag 8160

tctcactctg tcacacaggc tggagtgcaa tggcaggatc tcgcctcact gcaacctgtg 8220

cctcccaggt tcaagcattt ctcttgcctc agcctcccaa gtagatggga ttacaggcat 8280

gcgccacaac gctcagctaa gttttgtatt tttttttttc ttttttagta gacacagggt 8340

ttcaccatgt tggccaggct ggtctcaaac tcctgacctc aagtgatctg cccgcctcgg 8400

tgtcccagtg ttgggattac aggtgtgagg ttcttttaag ttttttacat ttttttcatt 8460

tttaaatttt aatctatata cttatttatt ttgagaccag gttatgagac tggctagtct 8520

ttgtattttt ggtagagtcg tggtttcacc atgtcgccaa ggctggtctc aaactgctgg 8580

gctcaagcaa tccacctgcc tcagcctccc aaagtgctgg gattacagga gtgagctacc 8640

acgcctggcc agttcttact gatattgagt ggctattatt atttccagtg gaggacttac 8700

tttatcctga agaaaaccaa gggcttgctg aaagatgaga ttatgtttat gaatgctcac 8760

tggtggaaat atttctgcac actcaagcta gttatgcttt tttatattca aaggaatatc 8820

ttagggtatt atgtgaacac ataagaattt aaggtaatag tagtaattat cttaaatcat 8880

attcatttag ttcacccaca attttattca atccccacaa actcctagta aggaaagagg 8940

ggcccagaat ggctaagtgt cttactcaag gttacttata ctcctcttcc taatgattta 9000

acccaagaga gtccatagca aagggtggta tgtaactgat ctagagccat gttgccatag 9060

ttttatgaac ataagaatca ctcggagagc ttaataatcc agactcttct cctaggatct 9120

cactcaaaag gtagggagtg aggaccaggt atctatttgt gttttcagca agaacctagt 9180

ataattgtga tgcatgtgtc ccacaggaaa ctctgctgta aagggatgta accctatggg 9240

gataagggca ggaataggca taggagccca aaaggctctt ctgctccagc acttgcccaa 9300

ggacaggtag atcacagatc actttctgtg ggcagaaatg tgcagggccc ctgacaccat 9360

ggttgaactt cttcctatgg cttttcttgc tttggctatc ttgggctagc ctgccccatc 9420

atcctggctg gtgtttagca gcacattcac attgaaattt gggaggaagg gtgggtgaga 9480

cctgctagat tccagcatgg acattaacca tgtaatctgc ttttcttcac tgcctcttta 9540

agtgctacta atggaaagat atgatctttt tttttttctt ttttgtgaat ggtgttctag 9600

attctccctg gtatataagt tagacttgag cttttcctca tgtatcccaa attctttgaa 9660

atgtaagaaa gggcccctca catagcatgg tctgattttc tgcagcagtg gctgtgtatg 9720

tcttttcctt tattcttcta tagctcagaa ggatcagaaa tagggcatcc agtgagtatt 9780

gcctctatag attaggaaga ggggaagggc ctggggaaaa gtagagaggg agatttttat 9840

taaaatagag accacatcta tgcttatggc actaatattt ttaagttgga gagtagctaa 9900

aaacaccttc ctttttctta ctttttcatt tttcaaaaga aaaataaagc caaatgaaca 9960

aggagaatgc cgatctgtaa aaagagcaag catgtttgac caataggtcc tgacaggtga 10020

gggctgctca ctcggtgtgc cagggaccaa atgtttcctg ctgtagcaac tttgcttgag 10080

gcaagaatct gatacttcaa actctgtatt caatattatt gagaagctca gcttgagcag 10140

ttttttccag ttttagggca gctttgagaa attaatctac ttcaaagaga tcccttagat 10200

aagaagcagg cactttgaag ctttttttgt tttctccaag attctaaatt atgaccctgt 10260

ccccatatat tatattggga taccaaaagt caaacaaaag gctgctttga cctgcagtaa 10320

ctgtgtcaca tcaataaagc tacaaagagg tttgggattt tcttttcctt tttgccaccc 10380

atctcttaaa agcatgagta atggtcacat attctcggag caaataaaaa cctgcactga 10440

gaatcaaagg tggttttaaa cttccctagc actgatagta acgaggttgt ctgattattt 10500

taaatcttac aaatctagga gtagaaatat taaagatatt ttatcaaaga tatacaaata 10560

ataaagatac tttgattgga aatccagttt ttggtctcag aaaacttcat ttccctatac 10620

ccattgccag aagttagtga aggcgtccca tagttgtagg gaaaggagtc cttgagatta 10680

attcaataat tccacaaata ctaagcacct tatgtgttcc agacacaatt ttatacactg 10740

gtaatacagt gtttgaatac aaggttctta ctttctcaga tggagctttc ttgtgggtag 10800

aaggaaacat aaaacaaaca agaagtcatt agtaacaaga gtctctgtta catatgaaac 10860

agaaccactt tagattgggt agtcataaag cagaccttat gagcaaatga cattcaagct 10920

aaaacctaga tgccaagaaa aggccatgaa gatctgggga aagagtagta ggccaaggga 10980

acaactggca cgatcacccc agagtcagaa ccagccggtg agttcctgaa gccgaggtgg 11040

gtagggtggg ggaccatcag catcagagaa gagactggag agaggcagag gctggattat 11100

ggagcctgtc agtttatggt aaggcacagt ggaaatacat cacaggactt tacacagagg 11160

catatttgtt ttaatttgca ttttaagaaa tcagtcttgt tgcttcatgg aaaatggatt 11220

gtagggagat aagcaacaag agtagaatta aaatggccct tagggaggca gttgggacat 11280

tgtggaggtg catagaagtg agcagacttg gggtattaag atgacactga tgaacttgct 11340

gagggattgg ctgtgttgag ggaaggagga accaaggatg acccttggac ttttggctgg 11400

agcagctggg tgagtagtag agtcactagc tgggatatag gaattctgaa agaataagtt 11460

tgaggatagc tgatggggat gggtaacaac aacttggttt caagcacact gagtttaaga 11520

tgcctatcgg acaggaaaat ggcagtgtcc gactggcacc tctgtgattc taaatttctt 11580

ttcttttctt ttcttttttt tttttttgag acagagtctc actctgttgc ccaggctgaa 11640

gtgcagtggc atgatcttgc tcattgcaac ctccgcctcc tgggttcaag caattctcct 11700

gcctcagcct cctaagtagc tgggattaca ggtacgcacc accacaccca gctaattttt 11760

gtatttttag tagatatggg gtttcaccat gttggccagg ctgttcttaa actcctgacc 11820

tcaggtgatt cacccacctt ggcctcccaa agtgctggaa ttacaggtgt gagccactgc 11880

acccggccta tgattcttaa atttcaacag agaaatcaaa tctggagtta gaagtttagg 11940

aatcactact gtcattactt tttgaaagga cttgagatta cttaagaaag tagacagagg 12000

gaagggaaga gatcctagga ccaggtacag ggtaatctga cattttagaa tgtaaaatag 12060

gaagatcctc caaaggacac agtgaggtag gtggaaaact tggggcctgg ggtgtcagga 12120

tgccctaaag aaagaaattt ttaaaggagg gcatagtgag ctgtctttat gccattgcaa 12180

gattgaatta agtaagttta ttgaagtgaa cagtaaagga ttctcttgga attacctaat 12240

gaacataata ttttgaaagt cagagaatct tgactttaat actctaggta atagtagggt 12300

gtctataata tagtgattaa ataaatacaa agatgaataa atgcaagttc agtgcaaatt 12360

ttaaattatt tttgttttta ttgtggagaa tttaaagaga taaattactc caggtatgta 12420

atttgttaga gagattgtat ccttgtttgt ttaggggagg gtaacacagg ggaaatgaca 12480

gctccccttc caacctcagg ctctattagc attttgagaa gccagctttg ccacatcacc 12540

atttctctta cttgtgtaat acaaggcaga gaatctttgc tctaccaggt ggaagatttg 12600

ctttttggtc tccaaaattg gaaggagtct cagcatccac tcttccaaat tttatagaag 12660

ggattattta tcccggaggc tctcagaact ctccctcaga atctactccc cataatgaaa 12720

gaaaaagact tcactgtgca aattaccaag tgtccttggt gctgcagggc aaaggctgta 12780

ggcaggttcc acagccttcc cgagagtcaa cactcatgag taactgcggt ggttggcaag 12840

agaagtaaac ggcagtggtg taatgtggat tcttttctcc tcccaccctg aaaaacccac 12900

ctgattaaat atttccttat ctggctttgt ctgtttacag ggctcatcca tgtccctttc 12960

acggtccaac agtcgtgagc acttgggagg tggaagcgaa tctgataact ggagagaccg 13020

aaatggaatt ggacctggaa gtcatagtga atttgcagct tctattggca gccctaagcg 13080

taaacaaaac aaatcaagtg agcgttgtgt tttatgcaca cttttaggta tccccagatt 13140

agaatgtgtt tatttgggtt ggagtatcac tgactgtggc ctctctgact tcaagtgctt 13200

ggtgtaattc acatgccatc tcctctgtga agcctccttg tttctcccag tgaagcaggc 13260

cacctggttc tctttcctct cactccattt tgtgcgtgct ttcactctag cacttgccat 13320

accaaagggc aattatttat ctacctatct gtatctcctt gtgaaatttg tgctccacaa 13380

accaaagact gtatttgttc attttataaa catttatgaa gtgtccatgt gtcaagtatt 13440

ctgttatgct ctgccttatt ggatatttct tcccaaacgc ctagtgaact gtctgacacc 13500

tagtaggtag gtgctcagta aaagtttgtg aaatgaatgc atgaatgggt aatgtacaca 13560

catgcatcca ggtggtgaca aaaagacaag gacataagaa agtggttgag gccaggcatg 13620

gcagctctca cctgtaatcc cagcactttg ggaggtgaag gtgagttgat agcttgagcc 13680

caggggtttg agaccagtct gggcaacata gtgagatttc atctctacta aaaaaaaaaa 13740

aaaaaaaaac aagaggctgg gtgtggtggt atgcatgcgc caatagtctt agcctacgtg 13800

ggagactgag gtgggaggat cgcttgagcc caggagtttg aggctgcagt gagctgtaat 13860

tgaaccactg cactacagcc tgggtgacag agcgagaccc tgtctcaaaa aaaaaaagtg 13920

gttgagaaaa atgtgattga attagcatct tagaccattg aagaaagact gtccagcggg 13980

tggctactaa aggtgaatag cagggtttga ttttgtaaat agacaagatg ttgctgaatt 14040

cgcataaaca ggaaaaaatt aacttattac agcatggggg ttctggcttt tagcaaatct 14100

ggctgagact cagcataagc ccattgtttt atctacaaca gcacccatac tcctgggctg 14160

tataaacaga tgagagctga cattgtcatc acaagatatg ccccaatgcc ttttttcagg 14220

gtttttgaca aatttggaat cctgaaacat gtagaatatt tgaagactcc ttgaagctag 14280

gcaatctaga tgagcatggt agacactcag taacagtcag ttgttacgtt actatcactc 14340

atgtcagctg tattatcagg agtctcagat ttgaaaggag taatcaggtt gttggccaag 14400

tctaaataag aaagcacgga caactatttc attcctaagt acactgtcaa tagtagcctg 14460

gagacttccc attagttact ggctggacgc tccaccactg acagtatgat caagatgaga 14520

gtcaggctga tgccaggcac agatggaggg gtgtaaatga tgtccagtct ctagcccaag 14580

atacattcat ttggtgcttc tctttttcta tctttatctc ctttcccttc atttaacatt 14640

ggataattaa attgactgta ctactcagtt gaatagaagt tcaggattaa gatggaggat 14700

acattaataa aaaggaatga ttaatgaagc acagttattt gggttttgtc acttttttta 14760

gggtataggg agtttaagag agactataga aactacaagt agttaagacc aaaaaaaaaa 14820

aatttaagta aaagtacgtt ataggctact ttgaacattt atcactatga acattttatt 14880

ttcaaacatt tgtttttaaa attgttaata atttaacata tgtaagattc cttttaaata 14940

tgtaatcaat ataaaaatta ttggagatag cttacattct ttcttaggtt gtacaaaggc 15000

tttaaaatct gcagtgtatt gtgtactgac agagcatttt cattcccact aggctcattt 15060

caggtgctca atagccatgt gtggccaatg gctactgtat tgaacagcac agttttagat 15120

aatttctctt aacacccttg tcaaggtcac caatgtttcc attttcctgg gcatccctat 15180

ttataccagt tgctgcagca taattctcag taactctttt actctcgaaa aaaaaagtaa 15240

atctatcatt tggaccataa attatatggg cagcctttca gccactttga tgtagtcaca 15300

tcccacatct ggtggtcact caccccaatt tgcttatcgc agtccacttt gtggctttgt 15360

gtcacctttg acccggtacc tcaatagtca ctcctcctca aactgttttt atgactttgg 15420

ggcctctgct tcacctttaa atggtggagt gtcccaggac tcagtatctt ggggtttggt 15480

tttgggtcct ttctctacac attcacccca agagataaac agcagctatc cttaagagaa 15540

aaaccttttc aggaaatgcc ttctgtttga gaatcataga ggacatctca attgggagat 15600

gaaaacaact ggagaaagtg atcaatgaca cccaaaacag tgcagaacaa gattcctaga 15660

tttggcagac acatggatcc ccagagtaga ctagatggat ctgattggct ttggtcagtg 15720

ttgtcctccc tcacctcttt ggggatctct cctgaagcca catccttggt gtaagaagac 15780

agatttccca cacaggcaag agctcattat tggtctggga ggtggaatgc ctgcactctg 15840

ctgaaatctc caaggctgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg 15900

tgtgtgactt acttggcttg gttcaatagt ccgtcaccag cgcttgcttg tcactgggtc 15960

tgagagccac agacagtttg ttgaaacctt tgcccaccaa atcagttgca gaaccaagga 16020

aaagttcagc attgctgtct taaaagtttt ccccagagac agatttattt ttgtcctaaa 16080

ttaaatccag gaaccccagc atcattctta tcactttata cattactatt aatcttggaa 16140

gtgtgctgtt ttgtgacctt gctacccagt tatatttttt agggagcagg atttatgtgt 16200

tgcaatacca tacaattcct ttgaccaaag gaaagcacag gtgtagatac agagagccac 16260

cctgccatcc aacggagcct ggacttgtaa taactttcca tacctttatt ttgtcccagg 16320

gatccttttt acagctcctc atttaattca catccaatca attttaaaca acaaatgctt 16380

ctgactactc agggagaata tgatgaatga ttattaacta cagaggcact gttgctgaat 16440

ttctgcctcc caagtgtaac cttttaaatg tcaagagcct ccctgaaagc aataaccagc 16500

atttccttaa tggaaagcca atatttcagt aaaggattgc tgtatttcta cagctactgc 16560

cctattcagc tgaacaatgg aagttgaaat atttaggtga ttaaaacttt taattaaacc 16620

atgttctctc tgggcattta cacttcaagc ctgtctctcc tttgcctttc agcggaacac 16680

tatctcagca gtagcaatta catggactgc atttcctcgc tgacaggaag caatggctgt 16740

aacttaaata gctctttcaa aggttctgac ctccctgagc tcttcagcaa actgggcctg 16800

ggcaaataca cagatgtttt ccagcaacaa gaggtcagtc attatttatt ttcccaagta 16860

tctttgtttt gattgtgtgt atgatgactt ctagaatgaa atcacttatt gacctatgag 16920

tatgctttca ctgagcattt attcaagtct catagcttgt ccttgctact aagaaaaatg 16980

tcatctcatt tcttatttag tgcagctagt aaacacggga tagcagaaat agagaaaatc 17040

atacttgcag aaaaatgtac aatagctgtc taacagtttt agcaataaaa cccaatcgat 17100

atatagattc ctaggaattt ttgtagtcac agctccctta aggtcagcaa caagtctgta 17160

tttaacatga tttcattttg aatctgttgt tatctttatt tgtggcactt aaaagtaaat 17220

aacagcatct gaaatatctc tggctgtttt tgactcatcc tggtcatgct gaagtctgtt 17280

cttacagaat atatgttttt ttcaacatgt taatttattc attaacttat tgtttgggtt 17340

ttacctgaac tgagacagaa ctgagatgtc tactgaacaa tcatgaccat ggattttggt 17400

caaactgatt agccatatcc tattatgtac tggcataaag aaaagaggcc ccaggcatga 17460

ttcttcctaa cagtgctctc atattactgg ggcacagcta actcaaagtg tcagaaaatt 17520

aaaatgtttc tatacatttg tgtctttttc aaatcaagac atggtccatg tttgatgcta 17580

gttggcttaa ttttatgtta gagaacatct gatttagtca gtaatttcta ggcacgtgtc 17640

agttaataaa gaatctatag tttttactag gcactttgta tcataaattc aatgtactgc 17700

ccatggtcca ataaattatt aagtttaaat gctgagcatt tttgtttttt acactgaaag 17760

ggcaattgtt gtgtgtatta aatttcaggg tattgatttt ttttttctgt ttcccctaca 17820

tctgagttag tgttcattta accaagaatt ttctaagctg aataaacctt tcataatcag 17880

tgcattaaag aatatatctc cttattttgt cttgctggtt agatgatggt agctggaagc 17940

agaagtattt taatgtgtcc aggcacagaa ggtcaaaaag caagagattg aatgttaatt 18000

caatgatatt ctgggtttgt tttcatctat gtttcctatt tgaagaagca tagaaattgt 18060

aggtatttct tattccaatt actccaagaa ggaaaaaaac atgaaagtat aaagccagag 18120

ggacttaatg taatatttta ttaactacag caggggtcag caaactgtgg tctgtaggcc 18180

aactgcctga ttttgtaaag ttttattgga acacagccac attcatttgt ttacatattg 18240

tctatggctt ccttccagct ataacagcgt agttaagtaa ctgcaacact gacattgtgg 18300

cccacaaagc ctaaaatatt taccatctgg tcctttaaga gaaagctggg gaacacttga 18360

gctgaagaat ctctattata gaatttctga aaacatatct ttagaactgt gataaatgat 18420

ggcctaaggt tttctaagac ctggatgtac cttccaaatc aaaacaggac aatttaaatt 18480

atcttcaata tcatttaatg aaaagtttta cctgtttatg gcatgttgaa atttggtcat 18540

ctgctttttt cagtgtagga tattttctaa tactcagatg tgatttaaca agaatccccc 18600

ataagtaact tcatgagatt taatttattg taagcatgtt gaagattcat ttagcaactt 18660

tcacaggtga tatatcaaca tgcattatta ctaaccatac ctgccaattg tcttatttgg 18720

taagcccagt actttatatc tatagttata gtgaatgctt acaacagttc tgcaagatac 18780

agtattgtca accctctgtg cccacagcta gtaatggcag agtcaggatc gaacccaagt 18840

ctctttggct tcagaatctg ttgtctttct cttcttacac actatcttta aaggaaggtg 18900

ggggatcctt gggcagtttc ccttcatgct ttgttcctga tgccagacag tgcagtttta 18960

tagttcctgc aaatacttct tttgtgtccc tttcagtgat agtttgctga gttgagccaa 19020

gtgaactgta gatctgattg tgtctgggaa ttcttctttc aatcctttca atcctgtgtt 19080

gaacacaaag gaagatgcca tctgttttat tatttagttt cttcttataa agataaagat 19140

acacttaacc aatttaaaat cccaacctaa gaaatatttt tctcttctct gaacagataa 19200

tgctcttcct tttatcagtt cacaaaaatg gtacaatctt tgtgtttcct ttcttgctat 19260

agttgccggg ttgctcagag ctaagtcttc tgctagaatc atcatttcca tttcaccacc 19320

tttcttaatt cttgtttctt aggtttgtgc taccaccttt cttttctgga agtaggcaga 19380

gcataattaa taaataagtg tgacaacaag ttgatcctac agtgggaaag aaaataagta 19440

attaatacat tggttataga ttgacctttc tacccacaga tcgatcttca gacattcctc 19500

actctcacag atcaggatct gaaggagctg ggaataacta cttttggtgc caggaggaaa 19560

atgctgcttg caatttcag 19579

<210> 2

<211> 13253

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 2

ctttaattag ctgcataact ccagggacta taattatcag aattgctgtg agcttgcaaa 60

aggttaagaa aatctggatc cgggcgctcc agctgacact catgctattt aggaccatca 120

ctacagctgc aaacaaatag aggaagttac aaaaggtgaa ttctcactgg cacaaagaca 180

ttttaggtcc ttgttactca aagggcaatc tgtgaggcag cagtgttgac attacttaga 240

agtgttttag aaatgcagaa tctcaggctc tgccccaggc ctaatgaata caaatctaca 300

tttcaacagg ttctccagct ggtttttatg cacattgaag tttgagaaac atggataaag 360

cctctggaca ataagaattc ttctcttgtt ctttgtatgt aagagtggac atatattgta 420

gtactctaac aatcacataa aaatgtcttc taaccaaacc cagcatccaa tcagatggct 480

gaatttcact gtctagcttg atgtgacaac tctttcctgt ctctatgatg gtatgcctgc 540

caatgtggat cacactcaag aaggagcaag aagtgtagaa gttggctcag atcttatttt 600

tcactctcag gactagcctc aggcagcact tttcttttgg ttgtcttctt tctaattagc 660

atgtcaaggg gcgactttca acccaccgta caagtatagt ttttcaagat atgtttcagg 720

gcttcagcac aatattctaa gtgatgagtg caaaagacta accaggctag aatgcacatt 780

gggttcaaag ccagaagaat ttttcattct tactgatgct gtctacaatt tcccaagcag 840

caataactat ccaccttcga caccttgtgg accatgaaaa cctcaaggat agagcaaggg 900

ttattcatcg ctgtatcgct ggtatccagc tccatgcctg gcccaggtaa cttaatacat 960

ttttgttgaa tgaatgaact ggatcctcag ttagaaaatc taaggcacaa gggaaatggt 1020

gatgctctaa gacctttctc ccctactctg ctgggatgac tgaggttatt ctagacagtg 1080

gttttcaact ttatactctt tccacaacct tatgcttgga acaacccaaa acactacttt 1140

attcctgaca tttacagtta ttaactataa cctttatatt tataaaggta tattctccct 1200

aaacaaatat ttaaaatgta aagatatttc tcaaagaatg aaacaaaact cctacagaat 1260

tcttattatt caaattgaaa ttggaagttt tccttatata tagtaatgag tccgcctcac 1320

agttttcact gcctgatata gttactagct cacttcagaa tttaacaatc aatgcaaatg 1380

tcagcaacac ataatcttta aaggcaaaaa aatggaaata gcctaaacct cctaaaggaa 1440

ggctaattaa ggtatatcat tttaatagtt tatatgcagc cacttaaaat tatgaggcag 1500

ttaaatattt attacaagaa aggatattca tgatatatta ctgatcaata cagcaagtta 1560

gaggaatgga tggaaagaag gaaggaagga agaaggaaaa gaaggaagga aggatgaaag 1620

gaaggaagga aggaaggaag gaaggaagga aggaaggaag gagaaataga tctggaaaca 1680

tatttaccaa aacatcaaca gcaggtgttt ataagtgata gaacactaaa taattttcaa 1740

ttttttaaat ttatttttag gaatagttta ataaatttta tgatgaacat atagcatttt 1800

ggtggctata gtttcaaata ctgaattgta tacttgaaat atacttaaat gttctcacca 1860

cagaacaaaa caaaaaaaag aggggtaact aaatgatgtg acagaggtgt taactcactt 1920

gattgagaca ataatttcac tatatatata tatatatata tatatatata tataaataaa 1980

atcattacat tgtacaccct aaaattacaa ttttatttgt tgtatatacc tcaataaaga 2040

tgaaaaaatt ttcaaaagta aatgaagcaa taaaatacat tttgtttttg gaaaaaactc 2100

caagatggga aaaataaggg atcagaagat ctcatacaaa gtgaaataca gggttactaa 2160

cgaagaaata tttcaaagga gtaattatga gaatgatcaa aaaggccaaa cccctgaata 2220

agctacaggg gcatctaagg ggggttattt catctgtgtt ggaagctgaa aacatgaagg 2280

gatagacagg tctgttgtca atgacaaagt gcattatttt atcagaggaa tcacaaaaat 2340

aaaattattt atctctcccc aatataaggc acttatatat ggaaaataag ttagtgtaac 2400

tctgctccct gctccatcat ccagtgattc ctttcctttt ctatgcaaat gactgtgtga 2460

tgagtcatta cacaataatg attattgttt taattaagga ggttcaacgt tggtgtaaaa 2520

gtgaaaaaat tgtctcatgt ggccaccagc aacaaaaggg cagtgttttc tttcaaatag 2580

ggctcctaaa acccaaagca ctctgtattt atgaaagttc tttccacagt agaaaagaac 2640

tgttatatag agagaaagag agaaaaagaa aggagaggaa aggagaagga taaaaacgaa 2700

aagaggcagg cagggaggaa ggaaggagag agagagaggc attcttattt ttggaacaac 2760

taactatatg tttgaaaata agaaagaggt tttgttactt tcataaagag tataagtacc 2820

taatgtctat tgaaatatta aaaaaactgg gtatttggga ttttaaaaaa tactttgtaa 2880

agttttctta ggcaaatgga agacaaaatt aaacagatat tatgaattga tcatctcacc 2940

ctgctttcct ctcagatagc ttaacaaaaa ttctcattaa gatgctagta cactgctggt 3000

gggaatgtaa attagtatag ccactataga aaacagtatg gaagtgtctc aaaaaactaa 3060

aaataaatct atcatatgat ccagcaatcc cattgctggg tacttgattg tggtgatagt 3120

ttcacaggtg aatacataca tcacacttta tcaaactgta catttaaagc atgtgtggtt 3180

tattgtatgt caattacacc tcaatgaaac tttttaagaa aaaaaaagat gaattttttt 3240

caaagggttc ttaaaaccct gctttatctg agcatccaga cattgtactc attatttggt 3300

acactaaatt ccagctgaga tgcccctgaa ttaccctgaa ggcacagttt aacaagtaac 3360

aaggcagcca gcgagaggta cagtgaagct tctccggtgg ctataactgc tgtttcatca 3420

cgtgtcttta cagtgaaatt tatgtctatg tatgttgctt ctccaacaat attgaaaagg 3480

gacttgggat tcactaaatg caagagcttt ctaagtttct gaaacaaata atatatactt 3540

ttaaaaaata ctaccttacc cacattgcaa taatgaaaga aatctgggcc agctctctgg 3600

tcctagtttt acttcctttg aataacaatt tgctaatttc acacatgagg aaataattat 3660

atcagtctgg gcattctttt gtatgctccc cttaaatttt accaaaataa aaatacaggg 3720

acatatagtt tttatttact tatttatgat gcagtttcct aagaactgaa atgtggcaat 3780

ggaagtgata tctataaatc ctggagataa gaaatacata tttgaaggaa gctatatttg 3840

aagaggatgt ttaggggaat gttaaggact gaagctgtga tttaaggact ggcaagtatt 3900

tctttctgca ctatgtaatt caagtctgtc tttgggtttt gaagagtgag attacagagt 3960

ctagatatat tgggttttct cccagggtca ggttcttctt ggcttctgaa ttctgagtac 4020

tttgtgctta actgataaaa tatatggata ttgtgtatgt atgttacatg ttgagataaa 4080

gtgggttgtt tcactgctgt tttgtaatga ctgcctggat tccttttatc tttttattag 4140

aattcatctc ctggatgcat taattttaga gaagacaggt agcaacaaac cctttaaaaa 4200

gcagagtgac agtttgtatc taggatctca ctattgggaa taaaaatgtt aagtgatata 4260

taaatataaa cttattttat ggcagacatt gtacaaagac tttacatgaa taatgttatt 4320

taatcctcat atctctatga catagtcatt ttattggctt tatttttcag atgagcagct 4380

gagactcaag atattgttta acttgcgcat gtttgcatag gtagtaacag tgaagatggg 4440

tctggagccc aggtaatcta actctggagc ccacactttt accattagtt cccaggcaac 4500

aatgaggaag tagttcaaaa gacatacatg atccccttgg tgtacctagt tcttttaaac 4560

tcttgatggc tctttctctc tgcaaaagtt cacatttatg ttagctacag gaccctttgt 4620

ttccacattt tgcatgtcat cctcctaaag tatgtgatag ttgttctgca gtacttgtat 4680

attttaatac tatgtttatt tacctaagaa ttggttgtgt tgctaagtta cttatggtag 4740

aaaagaagta ggtatagcca gatttcaaag atttaaacag agccacttca tatgctgtat 4800

ttgaagtgtt aacacagaaa tagtcctaga ttttctttta tgtaatttaa tggtgccagg 4860

aaaatataga tttcaaaaag aatataaagt aatacgtttg cagaactggg taattttttt 4920

cacaatacaa tcacaaatgt tatttttgct tgttcctccc aattactttc aaaagtttga 4980

gaattaggat ggggttctta actcatcttt attttatata gataaaataa actagcaagg 5040

aaattgacat ccaacaattg atggtgttag ggatggatct gggactagat ctcgtatctt 5100

aaggagaata tttttattat aatattctga cattcaagtg tataattata tagggtccaa 5160

tttcaggaat acagaacaga taaatatttt aaatattgag catacttcaa tgcttattga 5220

atctataaaa gcagtgaaaa ttcatgaact tctacagaag gtgaagaaag taattacagg 5280

tcgggcgcga tggctcatgc ctgtaatccc agcactttgc gaggccgagg cgggcggctc 5340

atgaggtcag gagatcgaga ccatcctggc taacatggtg aaactccgtc tctactaaaa 5400

atacaaaaaa attagcaggg cgtgatggtg ggcgcctgta gtcccagcta ctctggaggc 5460

tgaggcagga gaatggcgtg aaaccaggag gcagaccttg cagtgagcca agatcatgcc 5520

actgcactcc agcctgggcg acagagcaag actccgtctc aaaaaaaaaa aaaaaaaaaa 5580

aaaagaacaa acgaaagtaa ttaaatattg agctataaga gagcagccaa atgcaaatct 5640

ataagatttt ccatcttaac ttccacgcac ctgacatgtt tactggaatg gatgaaattt 5700

gtataagcaa aaactttttt atcttcatta aatttgacag aaatgaaata cagtacttaa 5760

aaaaatgggg gtgaattagt atttacttaa ccccccaccc ttatcccatt atcatctctc 5820

aaatggttgg ctaacatttc acaaaatttg aaattctttt tttctatgat gtagcaagac 5880

ccttcaggtt tgcccttttt ctatgcttaa ctagagattt caaatgacca cgaactgttt 5940

ccttccccag ggatcacagg gaagcaattc atgcaattca actctgtttt ctacatcttg 6000

gttaaccttc cacccctaac aggtagaaaa tcacctagac tcactttgaa gaaagcaggg 6060

tgtgagagat ttgcccctag ataaatttct ccttctctat ctccatctcc acccgctgtt 6120

gcaccattaa agctaaattt ttcttacaga cagtttgact ttcccaatta gggcactgat 6180

ttacattacc taccactgag tcataaggat atccagtttg ctttttaact gttgctgatt 6240

atgggtacac atttctttct ccgaatttca agcttgcaaa aggaacatca aaatttaaaa 6300

atgaatgtaa taacttatgg caaaaataga aggaaatgtc tgggttcatc taagatttcc 6360

tcaggctcct tgttaactat tccgtcacca atgtctacta cagaggtttt gtaaacccca 6420

atatggttat cctttcctag aactgtttta aagtttagtt tgagcagttt tcatttaatc 6480

ctcagagata cgaattttcc ctttaaaatg tttaattgaa ttgggataaa caagaaaaga 6540

gttatctaga cttataaaaa cataaatata gcattagtat tcatataagt ttaactccaa 6600

agtgagtaaa atttgtggat ttataaatgt ttacatgcaa tctacttagt cttctggtta 6660

tggcttttaa ttaataacta tcattgttaa tttacagagc tctcaaaaat gccacccctg 6720

ctcttctctt ttttaaatat aataattgtg atatgattct gcctcagtca ttcaagggac 6780

aagaaaatgg tgaatataac aatggctgtt agttcagtcc cttagtggaa cagtggactt 6840

atcaagaact ttctataggc aaggcactct gttaaaggtc ttaaaaatct gactgtgatc 6900

atgtcttctg cgggaacatg gatagagcta gaggccatta tccttagcaa actaacacag 6960

gaacagaaaa ccaaatactg catgttttca cgtataagtg ggagctaaat gatgacaact 7020

cattaacaca aaggggaact acagacactg ggcctgcttg agggtgtttg gaaggaggga 7080

gagaatcaga aaaaaatagc ttgggtacca ggtttagtac ctgggtgatg agacaatctg 7140

tacaccaaac ccccatgaca cgagtttacc tatgtaacaa actgcacatg tacccctgaa 7200

ctcaaaatga aagctaaaaa aaaaaaaaga aaaaataatg aaaaagaatg tgacccaaac 7260

aaaaaatttg gcttaacttc atcttccttc agctattcaa gatattaata gattatcttt 7320

tcataggcat tttcctaata tataaaactg gggcttaaat aggtttaaaa attagcacaa 7380

gatcacaaaa ctaagaattg taacaccaag cttcaattcc aggttgtttc cctccaaagc 7440

ctaaactgta accactatgc tcaaatggtt caaccctcaa aaacttagac tgctagatct 7500

gaacataata tgaagcaaac tctatttcat ttttatcatt ttcccactgc ctggaaatat 7560

actgaagata tctaatcctc caagtttaac aagtaaacag actatagact tttctgccgg 7620

agaaaaaatt cttagacttc tcagaaaatc aagtcaacaa atacttactg agcaccacca 7680

acaacatgct aggagctaag tgaggggctg gaaatacagc aatgaatagg tctctagtct 7740

cctggaacat caaatgatgt ttttccaaaa gtataaatag ttaccatttt ttattgtctt 7800

cttaataaat tgaataaaat aatgtctttg ctgccagtaa catggatgga actggaagtc 7860

actattttaa gtggaattaa agaaaaagaa agtcaaatac cataggttct cacttataag 7920

tgggagctaa ataatgtata cacatagacg tagagtgtga aataatagac atcggagact 7980

cagagagatg agagggtagg aggaggataa gggatgaaaa ttacttaata tctacgatgt 8040

acactatttg ggtgatggtt acactaaaag cccagactcc accactacgc aatatatcca 8100

cgtaacacaa ctgcactgta ccccctaaat ctatttttta aattttaatt aaaaataaat 8160

aaatgaatta caggcaataa gtaaattaca ggcaataccc caggcattgt cccattgaaa 8220

caccttagcc atacaaacta aaaattcttc cacgattatg cagttgagtt attcctgtgt 8280

agctttctct ttatgagtag ctaaaactcg gttaaatgtg atcatattga ttcttaagac 8340

agtgattcaa atggcaccta gaattaagtt atcctttccc atatttaagg atttggtacc 8400

tggcatcttt tggggaatta gagtcatata tggcttcata tcactcatgc aattttgtac 8460

atgttcttct ttttgtttct ttttttaatc aatgaatatt tgtgcccatt gataaaagat 8520

gaacaacttc ttaatttgga gaccataaag aaatctagtt agaaaatact actaataatc 8580

aatcttaaaa gaaaattctt ttcatgacca acaacaggca gctgacatta taacactggg 8640

agtgacagct gctaacacta caaaatgtat aagtacaact taaacaccca ctacataaag 8700

gtaactccat gattacacat gagatataat tcaatatata gctaatttcc aattcaagta 8760

gtctctaaaa taattggggt tttctcttta aaagtcttgt tcatgtataa tagacttaat 8820

attttactag taaggtgatt tttactattt aaaaagatta gccatcatat ctattgacca 8880

aatacaaaac tggcatggaa tacagtattg gatcccactc caaatccttc cagtagaaat 8940

ttggaactgt aagcaaaaag aacatgatgt gagaggcaat cccgcaatgc aaaaagtcta 9000

aagacacttt gtcttcaatc atttttgtgt tgtttttcct ttttcacata tatagctata 9060

atactcactt atgcccacag ctgtaatgag cttgatcgca agttcaggga tttcacattg 9120

aataaaaaat ggttccagaa tgtagcgtcc aaatgccagg gatatcacag cagtagctgc 9180

agggctaaaa aaaaatgtat atatttaggt taaccacagg ggaaaaaact gcattttcat 9240

taattttttc taccttgagt gtatttagca tatggaacat gttaaatatg aaagtccata 9300

gttttcagaa ttccgtactt gattaggttg ccatagacat tccaaatgaa gtacttcctg 9360

taacactggt ggctagattt taaacttcac atattcttgg gaacaagggt aagtactatc 9420

aacttttccc ctgctctccc atatttgact tttccttaaa aatctggttt attacatgag 9480

atcttgacag agctaggctt acaggaagcc atcttctttc tcaaaaagtc agtaagtaaa 9540

cttcagccat attatgaatt aattaatgcc atacagatct aatgtgtcag ggcatagtca 9600

aaagaatgaa cacaacactc aacctaaaaa attagtatta agtgttccct gcaaatacat 9660

gcctaaatat ttgacattgt tttatttttt ctgtacagac aaaaactcaa agtcaagtga 9720

ttattcttaa tcctatagta acattcagat ataaaatata aatttaaaaa taatagattt 9780

attataatct gatacaatta agaaaatata aatgagacaa gaatatgaaa taaattcagg 9840

tatatagaaa tataggtaat acatctttct attttaattc ttcaaatatt tttatgagaa 9900

aattgcacag accttttcat tttagatgta tgtatatatg aatgcatatt ttttccataa 9960

acatgcaagt ctgtgcaatt atctaatttc tttttttttt ttttgaaatg gagtctcact 10020

ctgtccccca ggctggagtg tagtggcacg atctcagctc actgcaacct ccgcctcccg 10080

tgttcaagca attctcctgc ctcagcctcc tgagtagctg ggattacagg tgcatgccac 10140

catgcctggc taattttttg tatttttaat agagacaggg tctcaccata ttggtcaggc 10200

tggtctcgaa cttctgacct gatgatccac ctgcctcggc ctcccaaact gctgggatta 10260

caggcgtgag ccaccgtgcc cggcagcaat tatctaattt cttatgccaa acttcctttt 10320

tagcttctct ttcttctcgt ggttacccaa cattcatgcc cgatccatag attatcagaa 10380

aaccaaccag cattacatgt tcccaacttt ggccggctga tactggagag cggcataagg 10440

tttttcattt tcttcttttc ctagacaccc tcccatcttc cccaaacaaa actgtttatc 10500

ctattctcca tcatttatac aattttttat gtataattta aaatctgatt atgtcatttt 10560

agtgctttca aaatatgttc aggatatgca aaaagccttc acattgcatt caaggctcca 10620

tctggtctaa gctctcccta cttcttcagt tagcttcttc ccactagaag gatatgaatc 10680

ttctctgcct gcagcctctg caactaattg actatgtttt atacttcaga tcttagttca 10740

attttccttt cccaggggaa aacttctcca acctatgact gggccagatc tcttggagta 10800

tattcatact gaagcccttg gcctgtcttc cacagtgctt atcacaatag caattttata 10860

tttacctatg tgatttctcg attgataatg atttcctccc acttgactct gagctctaag 10920

ggtacaaaat catatctatc ttaatcacag ttttacctcc agtgtttaga acagtgtcta 10980

tcatatagtg gaaacccaat atatattgaa tatatagtgc acacgcaaat tcggtttaat 11040

gacccctgct gtgtacagat agataatatt tctgtccttg aaatctgtgt tttacagatt 11100

atttctggca atactaactt aatctaaagg tataatttta atgggaagta cttaggaact 11160

atgggtatta gtaaaagaag agaaatattt acatacttag atattttgtc cctcctcatg 11220

tcatttgtta ttctgcctat ttaaaaggcc aactcagcaa tatgagtatg ttatgacaaa 11280

gagatatatg aacatatttt aaaagcttct agaatacaaa ggtactttcc tctgtcaaca 11340

gacacacaca cacagagaaa agtagttttt ttttttaaat taaagtaaca tttgattatc 11400

cgctacctgg gagcagaaat tgtggctgat ttcattttag atctcttttt attgccattt 11460

accattcagt agcagtttgt ggaataaata aattaatata tgaatatttt atctgtgcta 11520

ctgtaagata taagctatat ttatatggaa tgatcatgtt catcataatg ctaagcaatg 11580

agccaaagtt acacaactaa tacatggctg aagacggagt caagtccaac tgtattaagc 11640

tccaaagccc gccagcctga caggccagca gtcaataagt agtacatgca tttgaatgct 11700

gagtatgttg tacacaatat attgtaactt taaactttca attacttatt caaaaataat 11760

ttaatatatg ccaaaaaaag ggcactgacc taagtagtat atgggttaca aaaagatctt 11820

atctttgtat gcttgaaatg ttggcagttt taaaagggaa acaaaatata gacacctaaa 11880

atattaagga agaatagaag gtatcatgct caatgccaaa tgtgaagcag agatagaatt 11940

ttcagaagaa tttagagatg aaagaaataa cataaattag aataaacagg aaattaattg 12000

tgggaaagaa aggatttaag atgacctttg aagagcagat aagattgaga taaccaaaag 12060

gaatagaagt gcattcctga tagaggaatt tcaggaaagg aaaaaaatac ttattattat 12120

ttcattataa caattttaaa taatgcagag tgtttaaaat gaaaaagcct tcatgaagca 12180

tatactactt tgaagatctt tttttggtta aaaaaaaaaa gacttctaac actgtaaaac 12240

tcatgattag tgttgtcaat acttactagc actgtatttg gcacttagcc ataaaagctc 12300

acatgaactc tcaatcacat atgtttgata agtttgataa gagcaagttt ccctagtaat 12360

atccattaaa ttctgtaatc agactgtgca catcacattt tatcaaagta gttagcctta 12420

aggaactgtg atactctgaa atgctcatat gacatcataa gcttcccaca aagtcgaagg 12480

aaatttgcta ttcagagtga cttagcacta ttcagtgtga ttttaaacca agacaagcag 12540

gaatgtaaac acattgttac aacaataaaa tcaaaaaaga cttttggaac tgtagttcct 12600

aatctgttta atataaggaa atgagtcatt tgactctgtc atgcaaactc tgttttttat 12660

gaagtataag acttggtgac caactaagtt gtgaggcaca gaagtgtctt ggtcacaatg 12720

actaagtaaa gttaaatgcc ttaatgatat aatctacaat aagagaaaac ttcaatattt 12780

aatttggtct agttggtcaa aaggtatcca ggtgaatttt catgttcata ttctttcaag 12840

taagcatctt tctcctggaa gtgtcaaaga gaatagctga gggtcaaaga aaggtagata 12900

tttctaaaag tttggcattg ctggtgaact gcttgtcagt gataaaattg cattaaaaat 12960

atcttaattc agaaaggtac tttatcacag gctttatatc ctgaggtaga catgtttatc 13020

atgtagtcac atgacatgca tgtgtctaac cagttaaaaa taatcaaggt gattcataag 13080

aatgaattgg tttatttttt cttaattctt tctactatgc tcttaccgta ttatgaggag 13140

ttccacccag actcgtacaa aagctggtaa tggaccaaag acttccaaaa tatatgtgta 13200

atgacctcca gatttcttta tagttgttcc caattcagca taagacaaag ctc 13253

<210> 3

<211> 24620

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 3

gagttcctgt ccaaccctcc agctggtttt cccagcagcc gatctgatgc tgagaggaga 60

caagcttgtg atgccatcct gagggcttgc aaccagcagc tgactgctaa gctagcttgc 120

cctaggcatc tggggagcct gctggagctg gcagagctgg cctgtgatgg ctacttagtg 180

tctaccccac agcgtcctcc cctctacctg gaacgaattc tctttgtctt actgcggaat 240

gctgctgcac aaggaagccc agaggccaca ctccgccttg ctcagcccct ccatgcctgc 300

ttggtgcagt gctctcgcga ggctgctccc caggactatg aggccgtggc tcggggcagc 360

ttttctctgc tttggaaggg ggcagaagcc ctgttggaac ggcgagctgc atttgcagct 420

cggctgaagg ccttgagctt cctagtactc ttggaggatg aaagtacccc ttgtgaggtt 480

cctcactttg cttctccaac agcctgtcga gcggtagctg cccatcagct atttgatgcc 540

agtggccatg gtctaaatga agcagatgct gatttcctag atgacctgct ctccaggcac 600

gtgatcagag ccttggtggg tgagagaggg agctcttctg ggcttctttc tccccagagg 660

gccctctgcc tcttggagct caccttggaa cactgccgtc gcttttgctg gagccgccac 720

catgacaaag ccatcagcgc agtggagaag gctcacagtt acctaaggaa caccaatcta 780

gcccctagcc ttcagctatg tcagctgggg gttaagctgc tgcaggttgg ggaggaagga 840

cctcaggcag tggccaagct tctgatcaag gcatcagctg tcctgagcaa gagtatggag 900

gcaccatcac ccccacttcg ggcattgtat gagagctgcc agttcttcct ttcaggcctg 960

gaacgaggca ccaagaggcg ctatagactt gatgccattc tgagcctctt tgcttttctt 1020

ggagggtact gctctcttct gcagcagctg cgggatgatg gtgtgagtta aggacctgga 1080

ggtagggtgg ggacgtggtc tgtggactca ctaccagcct agggtatttg gcaagatctg 1140

gaggtcctgg ctccctcctt gttaagctgc tctctggaca gtgcctagcg agccagcaaa 1200

cagcctagtc tatcctgaga gggcagtggc ccttctggat gtccttcctc gtgctccctg 1260

ggctctgtca gctctccagg actccgggtc agtcttcagc ttggagccct ctttatctct 1320

actcctcata ccaaccttcc gcttccttcc tcaggtgtat gggggctcct ccaagcaaca 1380

gcagtctttt cttcagatgt actttcaggg acttcacctc tacactgtgg tggtttatga 1440

ctttgcccaa ggctgtcagg tactgtctgg gaatcaaggt acctggacta ggctcatagg 1500

gtttggggtt gctccactgc cctcaaacag ctttggggtt gctccactgc cctcaaacag 1560

ctttggggtt gcttggcttt gggtgtggag tgcggtggag gtcatcttgg acccagcatg 1620

actcctcact ctcaaaccct tcttgtccct tcagatagtt gatttggctg acctgaccca 1680

actagtggac agttgtaaat ctaccgttgt ctggatgctg gaggccttag agggcctgtc 1740

gggccaagag ctgacggacc acatggggat gaccggttag tgccctgggt cccaggcaca 1800

gagtttgtgg gagggtcatc acccattagg caggtgaata gtacttgctg cgtggttctg 1860

accactgtga gggttcctta tggttcaagg aggcagtgag agaaatgttt ataagtacca 1920

gctactctgt gtttaacacc agtagagaag tgatgaagct aacttacaac agcagacact 1980

atttcctcga ctggaatcta acttggcatt aacagttgca cctaatatgc aaccttatga 2040

cctttcaaca tattttttta tttctatatt ttttaatgtt tttttttcct tttcttcact 2100

tatcagaatc cagaaatcat tacaaatgac ctttctgtat atttaagtgt tttttttttt 2160

ttttttttga gacaaggtct tgctctgtca cctaggctgg agtgcactgg cacaatcacg 2220

gctcaacaca gtctcaacct cccaggctca agcgatcctc ccacctcagc cccgcaacga 2280

gctcaccttg caggttcaag cgactctcct gcctcagcct cccaagtagc tgggactgca 2340

ggcgcgcacc actatgcctg gctaattttt gtattttttg tagagatgat gtctggccat 2400

attgtccagg ctggtcttga actcctggac tcgagtgatc tgcccacctt ggcttccctg 2460

aaaccctgtc tctactaaaa atagaaaact agccaggcaa ggtggtgcat gcctgtaatc 2520

ccagctactt ggtaggctga ggcaggagag tcgcttgaac ctgcaaggtg gaggttgcag 2580

tgagccaaga ttgcaccact gcactccagc ctgggcaaga gggcactcca gcctgggcaa 2640

caagagcgaa actccatctc aaaaaaaaag gagaattctt cccacagagt actgaagact 2700

gactttattt tctcccagtc atctatgttg atggccaaga agctgcataa ataagttatt 2760

atcatttatt cagcaaatat ttgttgccgg gcgcagtggc tcacgcctgt aatcccagca 2820

ctttgggagg ccgaggcggg cggataacga ggtcaggaga tcgagaccat cctggctaac 2880

agtgaaaccc cgtctctact aaaaatacaa aaaattagcc gggcgtggtg gcaggcgcct 2940

gtactcccag ctactcagga ggctgaggca ggagaatggt gtgaacccgg gaggtggagc 3000

ttgcagtgag ccaagatcgt gccactgctc tccagtctga gcgacagagc aagactctgt 3060

ctcaaaaaaa aaaaaaaaaa atttgttgag tactcactgt gtgcaacgca ctatttggag 3120

tacttggatg tgtcggtaag caaaacaaag tttccttccc tcatggagcc acgttctagt 3180

ttggggggaa aagataaaca gattcagtaa ataagtgaat tatcaacata ttagaacgtg 3240

ataagaaatg tgggggaaag gaaaagtaga gcaggataag aaagggaatt gggagtctaa 3300

ggtgcatgac ggggggcaag ttaccacctt aaatcaggtg gtcagggtag acctcattga 3360

gagggtgaca tgagcaaagg cttggaggat gtgagggagt cagccatgtg ggttcctgga 3420

aaagaacatc cctggcagaa gaaacagcca gtgctgaggc aggagggggt ctcgtttgta 3480

aaatgagtgc agatgggctg agtggtgtga agggcatggg cctatttggg gcaggaaggg 3540

gagagccgcc ccttccccat tcctagcagt aggggttcct gtctgatctg gaagctgctg 3600

aactgaccac agatccagct gatctctgct gcctctccag gaggagaggg tggtgggagc 3660

ctttcctatg gtcaattgtg ccttttctcc cctgcagctt cttacaccag taatttggcc 3720

tacagcttct atagtcacaa gctctatgcc gaggcctgtg ccatctctga gccgctctgt 3780

cagcacctgg gtttggtgaa gccaggcact tatcccgagg tgcctcctga gaaggtacaa 3840

gggaatgaga gatgcaggaa aggagcttat gtggttgggg agcgggggga gcggggaagg 3900

gcctcaccag caccctgcct tggagtggga ggttaatagc agcatgttga caccagtatc 3960

tggagcagtg tttcccaaac ctcactgtac tgttacttac tgttgactca tttttttttt 4020

tttttttttt tgagacgagt ctcgctctgt cgcccaggct ggagtgcagt ggcacaatct 4080

tggctcactg caacctttgc ctcagcaaag gttcaagcaa ttctcctgcc tcagcctccc 4140

aagtagctgg gattacaggt gcccaccacc acgcccagct aatttttgta ttttttgtag 4200

agacggggtt tcaccatttt ggttggccag gctggtctca aactccagac ctcaggtgat 4260

ccgcctgcct cagcctccca aagtgctggg attacaggtg tgagccaccg cagccggcct 4320

gactcacttt ttatatttag cctggtccta agctactggt ttccagccaa gctgcacatt 4380

agaatcacct gagtgacctt taagaaaatt ctaatgtctg ccccgagtta attgcatcag 4440

aatctctagt gtttgccggg cgtgatggct cacgcctata attccagcac tttgggaggc 4500

tgaggcaggt ggatcacctg aggtctggag tttgagacca gcctgtccaa catggtgaaa 4560

ccccgtctct actgaaaata caaacattag ccaggcgtgg tggcatgtgc ctgtaatccc 4620

agctactttg gaggctgagg caggagaatt gcctgaaccc aggaggtgga gtttgcagtg 4680

agccgagatt gtgctactgt actccagcct gggctacaga gcaagactcc atctcacatc 4740

tgaatatttt caaagatccc ccaggtggtc ctttgagcaa ggagagtgag aacctgggtt 4800

cttggttttt tggttttttt tttttttttt tttttttttt tttggagact gagtcttgct 4860

cttgctttgt cacccaggct ggagtgcagt ggcacgatct cggctcactg caacctccac 4920

ctcctgggtt caagcaattc tctgcctcag cctcctgagt agctgggatt ataggcatgc 4980

gccaccatgc ctggctaatt tttgtatttt tagtagagac gggggtttca tcatcttggc 5040

cgggctggtc ttgaactcct gacctcatga tccacccgcc tcggcctccc aaagtgctgg 5100

gattaccggc gcaagccacc gcgctgggca agaaccagtg ttcttaagca agaatacctg 5160

tgaactgatg cattggcggg attggcaagc aagatgagtc atagaggggc ttgggtttca 5220

tcttaactga gatgataagc ctctaaagga tttaagtgga ggcatgacag cagacaggca 5280

ttttggattt ccctgacttc gctgtgatga atggagtgca ggagaaaaga ccgggtaatc 5340

aggctgctat agtattctag gcagggaagg gtgggctgaa ctgaagcagc tgcacgaggg 5400

atggaatgca gtggatcggt gcgagaagcc aaggattaac ctggtaggtg actggcggga 5460

gggagagcga gagaagggaa catcagagat ggccccacat ttcttactca ggagaaggca 5520

gtgccatttg ctgagaagga ttacaggaga agcaggtttt caagggtgtg gatgatgaac 5580

ataggggtgg agtggattgc agcctctgag ttgaaggcac tcagagtatt tcattcttag 5640

gtgtctcctg ccttgggtgg gtgtggctca ggaggcccac ctgccaaccc cgccccctca 5700

tgtggtgtat gtacctattg catgcatatc cccttccacc atacacactc actggttcct 5760

ctccagtttg gtctgttccc tttctctggt cagttgcaca ggtgcttccg gctacaagta 5820

gagagtttga agaaactggg taaacaggcc cagggctgca agatggtgat tttgtggctg 5880

gcagccctgc aaccctgtag ccctgaacac atggctgagc cagtcacttt ctgggttcgg 5940

gtcaagatgg atgcggccag ggctggagac aaggagctac agctaaagtg agttgagggc 6000

cagacgcagt ggctcatgct tgtaatccca gcactttggg aagccgaggc gggtggatca 6060

cttgaggtca ggagtttgag accatggcca acatggtgaa accccgtctc tactacaaat 6120

acaaaaatta gctgggcatt ggccgtgcac agtggctcac acatgtaatc ccagcacaca 6180

tgtaatccca gcactttggg aggccgaggc gggcagatca tgaggtcagg agatcgagac 6240

catcctggct aatacggtga aaccccatct ctactaaaaa tacaaaaatt agctgggcgt 6300

ggtggcgggt gcctgtagtc ccagctactc gggaggctga ggcaggagaa tggcgtgaac 6360

ccgggaggcg gaggttgcag tgagccgaga tcgtgccact gcactccagc ctgggtgaca 6420

gagggagact ccatctcaaa aaaaaagtga gttggggagg gggtcaacca tgtttctccc 6480

atggataggg tggcttttgg tactcatggt catgtccttt catctttatg tctgaaaaat 6540

actaattcct tcattcatta gttattcatt gagtgcccac tgtatgctag ccactgtgct 6600

acttgggggg tatagtggtg acagtggtct tgctttcatg gagcttatat tctacttggg 6660

gagatatttt taaaaagtac attttgcatt ttgtgtctgt tccttttttt tttttttttt 6720

ttaatagaga caaggtctca ctatgttgcc taggctggtc ttgaactcct gagctcaagt 6780

gatccttcca ccttggcctc ccaaagtgct aggattacag gcatgagcca gtgcgcccag 6840

ccttcacgta tgctctatga agacaattaa actgatttgc taaaagaggg agcaacttga 6900

ggtgggttat ttaggaaaac cctttttgaa gaagtgatag atgagacctg aggatgtgaa 6960

ggaatctact ttgtaaagat ctcagggagc ctgggcaaca aagcgagacc ttgactctac 7020

ttaaaaaata ttagccgaat gtggtggcat gaacctatag tctatttaca cttggttagg 7080

aggctgaagt gggaggatca catgagcctg gaagatcaag gctgtagtga accgtgatca 7140

cgctattgca ctccagcctg ggcaacagag tgagactgtc taaaaaaaaa caaacaaaaa 7200

actcagggaa gagaattcct tgcagagaga gtagtaaata caaaggttgc aaagtgggaa 7260

caaacttggg accatgcttg agaaagccgg catgctgggg gtagtagaat agcggtatag 7320

gatggggaac catatggaat gaaggtggtc aaagtgggaa gcggccagat cacacagggc 7380

cttataggcc atggtcaggg ctttggaaat gattcctagt gggagcccac tgccgtttgt 7440

taaacagagg agcaatgcaa tctgatttaa atttttaaag ccatgactgg aaacctactg 7500

agcaagccaa ggctggggct cctcagcatg ggagcaggta gcagaggctt tatgcctcct 7560

gggttccacc ctggtgctga gcatgccctc tctcaggact ctgcgagaca gcctcagtgg 7620

ctgggacccg gagaccctgg ccctcctgct gagggaggag ctgcaggcct acaaggcggt 7680

gcgggccgac actggacagg aacgcttcaa catcatctgt gacctcctgg agctgagccc 7740

cgaggagaca ccagccgggg cctgggcacg agccacccac ctggtagaac tggctcaggt 7800

gctctgctac cacgacttta cgcagcagac caactggtaa ggagtagtag ctgcagaggc 7860

cactcttgct ccttgcccta ggtcctctca ccctcttgag aaccagtttc cctctccaca 7920

gccctgagct ctcccttcat cttgtggcca tgggagcaca tgcaaagggc gaggccagct 7980

gttctgcagc tagcccttcc cagggcgcta tggcagatac tcatagtagg cccagcttaa 8040

gcacatcttc tccctgcagc tctgctctgg atgctatccg ggaagccctg cagcttctgg 8100

actctgtgag gcctgaggcc caggccagag atcagcttct ggacgataaa gcacaggcct 8160

tgctgtggct ttacatctgt actctggaag ccaaaatgca ggaagtgagt gtggctgctg 8220

tggggctcac cagaactggg tgtaggaatt actgtccctg ggcccccaac aagttggtat 8280

cagccctttt ttttgttcgt tatttttaga gatggggtct ctctgttgcc caggctggtc 8340

ttgaactcct gggctccaac aatcctccca cctcagcctg ccgagtagcc aggacgatag 8400

gcccacacca ctgtgccctg ttttataccc cgatcttccc atccagggta tcgagcggga 8460

tcggagagcc caggcccctg gtaacttgga ggaatttgaa gtcaatgacc tgaactatga 8520

agataaactc caggaagatc gtttcctata cagtaacatt gccttcaacc tggctgcaga 8580

tgctggtgag gggtaaatgg agtgtggcat gggcatctcc atggcttcct aagagtggaa 8640

cagggacccc tggtgaggga aaccctgacc ttgtagggta ggaggttgtg gaagcagtgg 8700

gaggttggcg taaagggcag aggatgttat ggaggaaggg aagttctctc cagatggccc 8760

atgctgagac agcagtcatt ttcttctggc ttaacagctc agtccaaatg cctggaccaa 8820

gccctggccc tgtggaagga gctgcttaca aaggggcagg ccccagctgt acggtgtctc 8880

cagcagacag cagcctcact gcagatccta gcagccctct accagctggt ggcaaaggta 8940

atggggtggg gcattgaggg ggacccatat aaacaaggac tagaccagaa acagctccct 9000

cgccttcttt ggcctgtgat cctcctgaga agccatgaaa gccagtgatg gaagcgcagg 9060

gctgtatcac cagtcttatt ttaaatccaa gctccctcat tcattagtgt atggccttgg 9120

gcaagttgct taacctaagc ccaagtttcc ttatctctaa aataggggcc atgctactgc 9180

ctcgtggcgt tattattcag tgcagcagtg catggaagag gtattgtgta aatggtatgc 9240

tcaaatgtta gctttagtct gggcaacacg gcgaaacccc gtctctacaa gaaataaaaa 9300

aaatatagcc cggtgcggtg gcatgtgcct gtaatcccag ctactcaggt ggctgaggca 9360

ggagaatccc ttgagccagg aggtagaggt tgcagtgagc caagatcgcg ccactgcact 9420

ccatcctggg ggacagagca agatgctgtt tcacgctatt tcattaaaaa aaaaaaagtt 9480

agctttaatt attagatgat ttgttaccta tcagaaaaat acatagatgc ctttagacac 9540

aattttacat tcttacattc ttatgtgtgc cttctttttt tttttcaaga tggagtctca 9600

ctctgtcgcc caggctggag tgcagtggcg caatctgggc tcactgcaac ttccgccatc 9660

tgggttcaag tgattctcct gcctcagcct cctgagtagc tgggactaca ggcacgcaac 9720

cacgcctggc tacttttttg tatttttagt atagacaggg tttcaccata ttggccaggc 9780

cggtctcgat ctcctcaggt gatccacccg cctcagcctc ccaaagtgct gggattacag 9840

gcgtgagcca ccgcacccag ctttccgccc cgcccccccg ccgagataga gtcttgctct 9900

gttacccagg ctggagtgca gtggcgtgat ctcagctcac tgcaacctcc acctcctggg 9960

ttcaagcgat tctcctggct cactcagcct cctgagtagc tgggactaca ggcgcatacc 10020

accacaccct gctaattttg tattattggt agagatgggg tttcactatg ttggccaggc 10080

tgatctcgaa ctcctgatct caggtgaccc gtctgcctca gcctttcaaa gtgctggggt 10140

tacaggcatg agccactgtg cctggcctat aaggccatgt acattcttat ttcagggagt 10200

ttcacaaaat tcctgaagct ctaccctaga ctccacatta ggaaacaaac aaacaaacaa 10260

aaagccttta tacatgctgt taactatgag ctattaaacc atatttctag caggggaagc 10320

tacctcagcc ctagatttga gacttggttc tgccacatac ctgttatatg aacttagaca 10380

agcaacttaa cccatgtgag tcttagcttc ctgtactgta aaattgggat tagacttgtt 10440

atgaaggtta aatgcactga tgcatgtaag cctgggcaca gtgcctggca catagtaaga 10500

actcagaaaa tcctggctat tgatgatgaa tggcatctag gctttaaaac agtaggggtg 10560

gggtgatgat tgcttcatct gggtaactac attccaccca ggccctgagg tcaaactaca 10620

gtccaaggtc ccaaagggct ggggagcaga gggtagaaat agaggcttga gcaggggtgg 10680

agtaaacttg gctgcttctg ctgaccagcc catgcaggct ctggaggtcc tcctgctgct 10740

acggattgtc tctgagagac tgaaggacca ctcgaaggca gctggctcct cctgccacat 10800

cacccagctc ctcctgaccc tcggctgtcc cagctatgcc caggtgagtg cccaaccagc 10860

ctagtctggg gactgcaggg ggcccgtagc tttagaggcc cacctctgag aagacaggat 10920

atcaaagggg cagcttctcg gccttttacc accacaactg gctggagaat tgtggagtgt 10980

ggagcagttg gaggcagctg taagaagggg agtcatcttt gtccttcctg caaaaccagt 11040

tgttcattaa gtttacagac tgctgcagga acctaccgcc agtcttgtat ttcctaactg 11100

gttctgttgc cctagttgat gcttcattgc tcatgtaaag ctatcacagt gtctcctggt 11160

tggtctcctt gcctccagtt tctccctttt ctacttcgtc ttcttaacga ccactggtag 11220

gcacaggtgt gatgtaatct ctttcctgct taaccctgtt cccttttgcc ccaataatac 11280

ttgccagcgc ttacggctgc agtgtttacc cctggataac tttgccacaa aatatctcac 11340

tcttactatt tttgcatcac tctagtatat tgactttgga aacagaagac atcattctgt 11400

ttatagcatt ctgtttttag tggtgtcatt tccatttcca tttccatttc cacttccacg 11460

tcaccctccc gagtaactgg gactacaggt acatgccacc acacctggct aatttttaaa 11520

tgttttgtag agacagagtc tcactatgtt gcccaggctg gtcttgaact cctgggctca 11580

agtgatctcc cacctcagcc tcccaaagta ctgataatac aggcatgagc caccgtgccc 11640

agctactgct tactctgtat atagctttaa tctaaaagtg gttaaccagg ctgagcgcag 11700

tggctcacac gtgtaatccc agcattttgg gaggctgagg tgggcagatc acctgaggtc 11760

aggagttgga gaccagcctg accaacatgg agaaaccccg tctctactaa aaatacaaaa 11820

ttagccaggt atggtggcgc atgcccgtaa tcccagctac ctgggaggct gagacaggag 11880

agcacttgat cccgggaggc ggaggttgcg gtgagctgag atcacaccat tgcactccag 11940

cctgggcaac aagagcgaaa ctccgtccta aaaaaaaaaa agtggttaac caagtgttaa 12000

atggacattt tagagagtga atcaataact cctatgcaga ttataatatc tgaaataatg 12060

taatatttat tggacatgtt gaacattttg cttgtaaata ctttactcca cttttttttt 12120

tttttttttt ttgagacgga gtctcactct gttgcccagg ctggagtgca gtggcgcgat 12180

ctccattcac tgcaacctct gcctctcggg ttcaagtgat tctcatgcct cagcctcccg 12240

agtagctggg attacaggcg cgtgccacca cacctggcta atttttgtat ttttagtaga 12300

gatgggggtg tcaccatgtt ggccaggctg gtcttgaact cctgacctca ggtgatccgc 12360

ccaccttggc ctcccaaagt gctgggatta caggtgtgag ccacatggcc acccttattt 12420

actccagttt tgtttgaaga gcctggctgc ctttcctcat gtgccctctg attgcttcct 12480

tagttacacc tggaagaggc agcatcgagc ctgaagcatc tcgatcagac tactgacaca 12540

tacctgctcc tttccctgac ctgtgatctg cttcgaagtc aactctactg gactcaccag 12600

aaggtatttc tcactttctt aaactccgaa ggccctgggt attagaaaga atttaggatt 12660

ttcttgatat ttgcctggct tttgagattt ccctggagct aggcagtatg ttcctgaccc 12720

tgagaggcag gcatatttcc tagccttgcc ctcgccagcg atttgctcac cagtcctcct 12780

ctggtagaga tacagtcctt ttccttcttc aggggcaagg gggaaagtct gtcctgtgac 12840

gaaaagctgg gacaatggtg atcctgggaa ggcatgggag ctctggacta gaaatccagg 12900

ggatggcaaa gacttgagtt ttctcccaaa ctagttgtgt gacttgggca cgtcttctac 12960

cttctcatgc tctagtttct tctctacgtt tttatcattc ctaaattggc ggacaagtca 13020

ctggtgaggg caaagctagg aactgttact gcttctcagg gtcaggaaca ttctgcctag 13080

gtccagggag atctcgaaag ccaggcaaat attcagaaaa ttctaaaatc tttctaatac 13140

ccagggcctt cagggatggg gccacgtaat ctccagggcc tctcaagctc tggagtgcct 13200

gactgcctta ctgcctcctc tggctccttc tctccttcag gtgaccaagg gtgtctctct 13260

gctgctgtct gtgcttcggg atcctgccct ccagaagtcc tccaaggctt ggtacttgct 13320

gcgtgtccag gtcctgcagc tggtggcagc ttaccttagc ctcccgtcaa acaacctctc 13380

acactccctg tgggagcagc tctgtgccca aggtgaaaga atagggtgga tggcccccct 13440

tggatgacat gtatggtctg tctgctgtca gctcttctca aacctcatcc cctctgctgg 13500

ctaactatgt ggcccagcct acctagaacc tgcacagagt aggcctggga tagcagatgg 13560

gtttcagttt gcctgctgac tctaaacaat tggtgactgc ctggaatact aggctgaaag 13620

gatttttttt tcttttcttt tttttctaag acggagtttc gctgttgtca cccaggctgg 13680

agtgcaatgg tgcaatctca gctcactgca acctctgcct cctgggttca aacgattctc 13740

ctgcctcaac ctcctgagta gctgggatta ctggcatgcg ccactacgcc cgactaattt 13800

ttgtattatt agtagaggtg gggtttcacc atgttggcca ggctggtctc aaactcctga 13860

cctcgggtga tccgtctgcc tcagcctccc aaaatgctgg gattacaggc ttgggccact 13920

gcgcccagcc agctgaaagg attctgagac cccaggggat ctcagaggga aggagttatg 13980

agattgatta gctctgcctg ccttaatgca gaaggaagca tgggaagcag gctttgctgc 14040

cttagtagct aaaagtggtg aagttctggc tgcatcttct ccctttttca ccccacccac 14100

cacaggctgg cagacacctg agatagctct catagactcc cataagctcc tccgaagcat 14160

catcctcctg ctgatgggca gtgacattct ctcaactcag aaagcagctg tggagacatc 14220

gtttttggac tatggtgagt ctggggagga cagcagggcc ctcttggaat ggacaggcta 14280

tgtgagaggg ctgcggtttt ttctccagag gatccacact gtggctgagt gatggtggtc 14340

ttgtctcttt tgctacaggt gaaaatctgg tacaaaaatg gcaggttctt tcagaggtgc 14400

tgagctgctc agagaagctg gtctgccacc tgggccgcct gggtagtgtg agtgaagcca 14460

aggccttttg cttggaggcc ctaaaactta caacaaagct gcagatacca cgccagtaag 14520

tacagggcca gaggatatgg caatgatggc accaaagtgc catgcaccct tgaacatgga 14580

ttccaaactg ttccacagat tacatgctat gtttagatac attcgtggaa aaaggcattc 14640

cacagctaaa taagtgggaa atgctgagtt aatcaaggtt agatgttttt atttcctgta 14700

ggactctcgt gtccttcagt atgcggatgt gccttgcaaa tctccaaaag ggagatctag 14760

tgtacagtgt ttccctaaga atgttttccc acaggattct tttattgagg aatgcagttt 14820

gggaaatcct gtcccaaggc acaaggagag ttgggattag agagaagtgt agagggtttt 14880

ccccagggag cctttaagtc agcttaaggg agtgaatgcc ttttggaggc agggaaggac 14940

tcaggtagct tggcctggga aagaggcaaa gagaaagact ctccaggaaa acaaaggatt 15000

cctctgattg gttctcctct cctctctcaa caaggtgtgc cctgttcctg gtgctgaagg 15060

gcgagctgga gctggcccgc aatgacattg atctctgtca gtcggacctg cagcaggttc 15120

tgttcttgct tgagtcttgc acaggtgagc agccatgtcc ccatgaccat aggcggtgct 15180

gaaatgacac acactacagc acatctttct atgtaaaggt ttcgttgcct ttttttattt 15240

ttgagacaga gtctcacttt attacccagg ctggagtgca gtggcgtgat cttggctcac 15300

tgcaacccct gcctcctgcc tcctaggttc aggtgattct cctgcttcag cctcccaagt 15360

agctgggact acaggcatgc gccaccacac ccggataatt ttttttattt gtagtagaga 15420

tgaggtttca ctatgttggc caggctggtc tcgaactcct gacctcaggt gatccacccg 15480

cctcggcctc ctaaagtgct gggattacag gcatgagcca ccgctcccgg cctttgttcc 15540

ctttttaatc agatactgac ttctggtccc agggcaacat tcccttgact ttagtccaac 15600

cattggtctc cctctttcag agagcctgtc ccagacttac cctagttcta gttttcagtg 15660

cttcctaata cattttggct atttaaattt gatttagata atatttaggg ccgggctcgg 15720

tggctcatgc ctgtaatcct agcactttgg gaggctgagg caggcggatc atggggtcag 15780

gagttcaaga ccagcctggc caacaaagtg aaaccccatc tctactaata atacaaaaat 15840

tagccaggca tggtggcggg cacctgtaat cccagctact cagaaggctg aggcaggaga 15900

atagcttgaa cctgggaggc ggaggttgca gtgaggaaga ttgcaccatt gcactccagc 15960

ccgggtgaca gtgcgagact ctgactcaaa aaaaaaaaaa aaaagaagaa agaaagaaag 16020

aaagataata tttagaattc agtcctcagt cacactagcc acacttcatg tgctcaatag 16080

ccacatggct agtggctacc gtattgagta gcacaaacat atcctcagga gggctgaagc 16140

taataataaa acattgcatt gatggcacat tttgtctccc caatcacata tacttctagt 16200

acttcatttg ccccacacta gacctcataa agctgagtgg gagcatagat tattttcatt 16260

tgttaaatga gaaagttgag aagtctatgt ttggggccag catcacaggg taggaggcag 16320

gattcaggtg tgggcctgct ggggccagcc tgcttttctc agccatgtgg cccctgagct 16380

gcaggtgtgg gggctttgtg gcctgcattt gatccattgt tggttggaaa gaaaacaaaa 16440

attttacctc caaggcgggc ggatcacaag gtcaggagat cgagaccatt ctggctaaca 16500

cggtgaaacc ccgtctccac taaaaaatac aaaaaattag ccgggcgtgg tggtgggcgc 16560

ctgtagtccc agctactcgg gaggctgagg caggagaatg gcatgaaccc gggaggcgga 16620

gcttgcagtg agctgagatt gtgccactgc actccagcct gggcgacaga gagagactcc 16680

atctcaaaaa aaaaagaaaa aaagaaaaag attttaccta aaaaataata agccatacta 16740

atatttgata tgttatacag taatacatat atatacacac atatatatac acgtacgtat 16800

ataaaacaca tatattagag aattgggccc caaggtcgct caggaagtgc ttgatgccat 16860

ttctccccgt tttgtaattc ttgttctgcc ttctccccag agtttggtgg ggtgactcag 16920

cacctggact ctgtgaagaa ggtccacctg cagaagggga agcagcaggc ccaggtcccc 16980

tgtcctccac agctcccaga ggaggagctc ttcctaagag gccctgctct agagctggtg 17040

gccactgtgg ccaaggagcc tggccccata gcaccttcta caaactcctc cccagtcttg 17100

aaaaccaagc cccagcccat acccaacttc ctgtcccatt cacccacctg tgactgctcg 17160

ctctgcgcca gccctgtcct cacagcagtc tgtctgcgct gggtattggt cacggcaggg 17220

gtgaggctgg ccatgggcca ccaagcccag ggtctggatc tgctgcaggt cgtgctgaag 17280

ggctgtcctg aagccgctga gcgcctcacc caagctctcc aagcttccct gaatcataaa 17340

acacccccct ccttggttcc aagcctcttg gatgagatct tggctcaagc atacacactg 17400

ttggcactgg agggcctgaa ccagccatca aacgagagcc tgcagaaggt tctacagtca 17460

gggctgaagt ttgtagcagc acggataccc cacctagagc cctggcgagc cagcctgctc 17520

ttgatttggg ccctcacaaa actaggtggc ctcagctgct gtactaccca actttttgca 17580

agctcctggg gctggcagcc accattaata aaaagtgtcc ctggctcaga gccctctaag 17640

actcagggcc aaaaacgttc tggacgaggg cgccaaaagt tagcctctgc tcccctgcgc 17700

ctcaataata cctctcagaa aggtctggaa ggtagaggac tgccctgcac acctaaaccc 17760

ccagaccgga tcaggcaagc tggccctcat gtccccttca cggtgtttga ggaagtctgc 17820

cctacagaga gcaagcctga agtaccccag gcccccaggg tacaacagag agtccagacg 17880

cgcctcaagg tgaggtggga ctgttgctag gtggtggtga tggtgttgga tggggttagt 17940

cctggaggag agtgttttat agagcaggtg tccctgtgga atagctcccc agggcctagt 18000

ggaactttaa tctcctgcta tctgcagtac cccaatatct tgcttttttg ttttttgttg 18060

tttttttgag aaggagtctc gctctgtcgc ccaggctgga gtgcagtggc gcaatctcag 18120

ctcactgcaa gctccgcctc ttgggttcac gccattctcc tgcctcagct tcccgagtag 18180

ctgggactac aagcgcctgc caccatgccc agctaatttt ttgtattttc agtagagacg 18240

gggtttcact gtgttaacca ggatggtctt gatctcctga cttcgtgatc cacccgcctc 18300

ggcctcccaa agtgctgggg attaccggcg tgagccacca tgctcagcct cttttttttt 18360

ttgagacgga ctctcactct gtcgcccagg ctggagtaca atggtgcgat ctcggcttac 18420

tgcaacctcc acctcccggg ttcaagcaat tttgcctcag cctcccgagt agctgggatt 18480

acaggcgggt gccaccacac ccagcttatt tatgtatttt tagtaaagac ggggttttac 18540

catgatggcc aggctggtct caaactcctg acctcaggtg atccgcctgc ctcagcctcc 18600

caaagtgctg ggattacaga cgtgagccac tgtgccaggc caacatcttt cacagcaaga 18660

cctatttcca agtctccctt tgtttcagac agagacattt tccttaactc taatgtttct 18720

tttacctttt catcccacct tctgttccct cattttctcc ctccttccac acccaagagt 18780

cgctgcccct gatctagagt gttggctgtc atgtgagaag ttagttctga aatcttccag 18840

ggtcctgtgt gatggtgcct gatggtgcct ccactacgcc acctgctgtc acaaggtgtc 18900

ttgtcactga agacctctta gcccttcacc ctttcactct gatctcaggt gaacttcagt 18960

gatgacagtg acttggaaga ccctgtctca gctgaggcct ggctggcaga ggagcctaag 19020

agacggggca ctgcttcccg gggccggggg cgagcaagga agggcctgag cctaaagacg 19080

gatgccgtgg ttgccccagg tagtgcccct gggaaccctg gcctgaatgg caggagccgg 19140

agggccaaga aggtggcatc aagacattgt gaggagcggc gtccccagag ggccagtgac 19200

caggccaggc ctggccctga gatcatgagg accatccctg aggaagaact gactgacaac 19260

tggagaaaaa tgagctttga gatcctcagg ggctctgacg gggaagactc agcctcaggt 19320

aggacagcaa gggtgaggtg gaaggtgcat gttttggggg tttgttctgg ggcaaagcac 19380

aagcagtaag tgctgccaag gaagtacagg aagaatgttc ttttgctgac tctaagggag 19440

tggattacag aaggaagaac aaagagaatg gcagggggag ggagcactgt gaaaaaggcc 19500

tgctctctcc ccaggtggga agactccagc tccgggccct gaggcagctt ctggagaatg 19560

ggagctgctg aggctggatt ccagcaagaa gaagctgccc agcccatgcc cagacaagga 19620

gagtgacaag gaccttggtc ctcggctccg gctcccctca gcccccgtag ccactggtga 19680

atatgcgacc cctgatgttg gtcacttgga gagggctgag cctctagggc ttttgacccc 19740

tctgtctttc caggctgggt tcggatctgg atccagtagc ctctgaacct gtgtttgaac 19800

cccagcactc tgtctatgag ctgtgtgcag gtcacttaac ctctccaagc ttctattcct 19860

tctgtaaaaa tggggtttag ggagcatggt ggaataagtt gatgcctgtt agttgcatgg 19920

caccccactt ggcacattgc ccttcataaa tggtagttgc tgtttgccat gtgggagatg 19980

agatagggac tgttccttct tggaaagttc ctatcaacta tgaaataagg aattcagctg 20040

taccaagtgt cctgacgttc ttctaggtct ttctaccctg gactccatct gtgactccct 20100

gagtgttgct ttccggggca ttagtcactg tcctcctagt gggctctatg cccacctctg 20160

ccgcttcctg gccttgtgcc tgggccaccg ggatccttat gccactgctt tccttgtcac 20220

cgagtctgtc tccatcacct gtcgccacca gctgctcacc cacctccaca gacagctcag 20280

gtgggtgctg accatcccca agactcctgc tggggcagac tagagagaag gtccagactt 20340

tgaaggaatg ggacctcacc cctccccgtg ttgcttgcag caaggcccag aagcaccgag 20400

gatcacttga aatagcagac cagctgcagg ggctgagcct tcaggagatg cctggagatg 20460

tccccctggc ccgcatccag cgcctctttt ccttcagggc tttggaatct ggccacttcc 20520

cccagcctga aaaggagagt ttccaggagc gcctggctct gatccccagt ggtatgcggg 20580

cagccttctg gccggctcct ctgtcctctt ctgcatcttc ttacttggga gctgggtgaa 20640

ggagttttag gcattggtta gtttacatag atttatgacc cttatgtcat accttttcac 20700

ctgttagcat cttacatcag gaaggtttgt tgtttttttt aaacctcaac tttgaattac 20760

cagcaaattc agtgtctttc ctcagaggtg agttcagatg actggggtct tctgtgctaa 20820

catgaaacat attcacatga tttgagtgcc tcactttgtt agttgctgtg agggacacaa 20880

agtacaagtc atggtctctg tgcacaggga gctattagag caagggagag caccttgacc 20940

tctagtgacc agggaaggct tcttgatgct ggcttgagtg atggatagga attgagtgcc 21000

caagacaggg aagggaattc ctttaacagc tgaatgagtc tggctgtatc ctgtgtgtca 21060

ggggtgactg tgtgtgtgtt ggccctggcc accctccagc ccggaaccgt gggcaacacc 21120

ctcctgctga cccggctgga aaaggacagt cccccagtca gtgtgcagat tcccactggc 21180

cagaacaagg taggattcct gggccatgga gcaaagttgg gctggattgg gcttcgggtc 21240

aagctgctca cattctgtgt tgagggaggg agagatggtg atcacgtgga caagcagagc 21300

tctcacagcc ccatctccca aagcttcatc tgcgttcagt cctgaatgag tttgatgcca 21360

tccagaaggc acagaaagag aacagcagct gtactgacaa gcgagaatgg tggacagggc 21420

ggctggcact ggaccacagg atggaggtgt gtgcttctgg ggtggggtca ggcctgctct 21480

aggaatgacc cggggggtcc cagtgacttc tctaccagac ggcctgggtc agtgctaaag 21540

ccacttcaaa tcccttctgg aagtgtagac ctaaatttaa aaatatgtca cctggggaga 21600

acttcagagc ccagaacttg aagaatatat gggaatggat atttaaagat acgcagaaaa 21660

aaaacgactt aaacccaact atcagcttcc cagcagaaag atacgtaaga cagacatgca 21720

cattggaaaa cgcattttct catctccaaa atgtcagtgc ctgtgagaaa gaagcttggt 21780

ttcctctctg actgaagggt ctgccctctg cattcaggtt ctcatcgctt ccctagagaa 21840

gtctgtgctg ggctgctgga aggggctgct gctgccgtcc agtgaggagc ccggccctgc 21900

ccaggaggcc tcccgcctac aggagctgct acaggactgt ggctggaaat atcctgaccg 21960

cactctgctg aaagtgagtg aggaaagcag ggaagggggc caggcccagt ggcttgcacc 22020

tgtaatccca gcactttggg aggccaaggc agatggattg cttgagctca ggagctcgag 22080

accagcctgg ccaacatggt gaaaccctgt ctctacgaaa atacaaaaat tagctgggca 22140

tggtggcagg cacctgtaat tccagcttct cgggaggctg tagcaggaga attgcttgaa 22200

cctgggaggc ggaggttgca gtgagctgag atcgccgcac tccagcctgg gtgacagagt 22260

gagactccat ctcaaaaaaa aaaaaaagga taggcccctt ggcttacgcc tataatccca 22320

gcactttggg aggccgaggc aggtgggtca caaggtcaag aggtgaagac catcctggcc 22380

aacagggtga aaccccatct ctactaaaaa tacaaaaatt agctgggctt ggtggcacat 22440

gcctgtagtc cgagctactc aggaggctga ggcaggagaa tcgcttgaac ctgggaggtg 22500

gaggttgcag tgagctgaga ttgcgccact gcactccagc ctgggcgaca gagcaagact 22560

ctgtcaaaaa aaaaagaaaa cagggaaagg gaagaaactg gacttaatcc tttcccagca 22620

gctatcatga atgaagatgg tgctcaccac cactgtttcc ctgcagatca tgctcagtgg 22680

tgccggtgcc ctcacccctc aggacattca ggccctggcc tacgggctgt gcccaaccca 22740

gccagagcga gcccaggagc tcctgaatga ggcagtagga cgtctacagg gcctgacagt 22800

accaagcaat agccaccttg tcttggtcct agacaaggta aggagctggg gcagaggggc 22860

agtgtctagt ggggagtgaa taccaactca tccccatgcc ccttctgact tctgcatata 22920

cctggctggg gacagtaacc tcttagtgct ttttgcccag gacttgcaga agctgccgtg 22980

ggaaagcatg cccagcctcc aagcactgcc tgtcacccgg ctgccctcct tccgcttcct 23040

actcagctac tccatcatca aagaggtggg gttcagggcg tagtgtctgg ggatgactgg 23100

cgactgggga agacgtcaac aaagaagggc agagaaacct gagaagatag gagagggtcc 23160

taggaatggc tcagacatgg aaaggggctg agattgttag agcttgggcc tcttggtgag 23220

acaagcatcc taatcgccag tgtctcctcc tcagtatggg gcctcgccag tgctgagtca 23280

aggggtggat ccacgaagta ccttctatgt cctgaaccct cacaataacc tgtcaagcac 23340

agaggagcaa tttcgagcca atttcagcag gtcaggggcg cgaagacaag aagacgtgtg 23400

gggaagggta gacaacatac aggggcaaca agccttttct ccagaaacag ctgttgcagc 23460

ccaccttcta tctaatgatc cctctgctgt ctttgccacc tgacccctgc catgcatttc 23520

cctattctca cacctgcctt ttccctgcag tgaagctggc tggagaggag tggttgggga 23580

ggtgccaaga cctgaacagg tgcaggaagc cctgacaaag catgatttgt atatgtgagt 23640

gcttaaggca gggatgtggg gagaggggca gtcctgagga tggtatcacc atgggttgct 23700

ttgggacttg agagcctctg aagacacagg cagaggccag gtattactag ctcaagactc 23760

atctcacctc cttctgcctt agctatgcag ggcatggggc tggtgcccgc ttccttgatg 23820

ggcaggctgt cctgcggctg agctgtcggg cagtggccct gctgtttggc tgtagcagtg 23880

cggccctggc tgtgcgtgga aacctggagg gggctggcat cgtgctcaag tacatcatgg 23940

ctggttggtg agtctccaag ggcaagaccc atcctagggc attaggactc ctgccctcac 24000

cccaggttct ttcccaggtc tgaatcttgc ctctcttgtg ccccattttc ctcctatcct 24060

agttagttcc ctggcatgcc tggaccatta acccttagct cccttctgtt cttctcttgt 24120

aaccaagggc caaaggagtt tctcattggt tcaatcctct ccactcaccc acccccacca 24180

ccaatggtgt tttcctatgt attctgtttt agagccctta ctttgtattt cctccttttc 24240

ttttcccagc cccttgtttc tgggtaatct ctgggatgtg actgaccgcg acattgaccg 24300

ctacacggaa gctctgctgc aaggctggct tggagcaggc ccaggggccc cccttctcta 24360

ctatgtaaac caggcccgcc aagctccccg actcaagtat cttattgggg ctgcacctat 24420

agcctatggc ttgcctgtct ctctgcggta accccatgga gctgtcttat tgatgctaga 24480

agcctcataa ctgttctacc tccaaggtta gatttaatcc ttaggataac tcttttaaag 24540

tgattttccc cagtgtttta tatgaaacat ttccttttga tttaacctca gtataataaa 24600

gatacatcat ttaaaccctg 24620

<210> 4

<211> 341

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 4

tacctaaaaa taactgagat acagacctcc tgggaagggt ggcatcaggt atggctgagc 60

aagctgggcg agacttcatg gaagaggtag gacttgagct gaaaaggtgg gtggacccag 120

gtttatgagg gaaaaggaat gagttgtggg gcactctacg agatgtgtgt gcctttgtat 180

acatgcagtg agaaaaatgg cccaacctag aggaagttgt gtggcaagag gtgggaagat 240

aggcatgttg aattacatat ggatcagttt gaatgactag aatgagaagc tgggtttaga 300

aatgatttga ccaactctac ataaaggaaa gttgaattaa g 341

<210> 5

<211> 23

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 5

gatcaggatc tgaaggagct ggg 23

<210> 6

<211> 20

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 6

acaggaaacc ttggagggac 20

<210> 7

<211> 20

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 7

catgagtgtc agctggagcg 20

<210> 8

<211> 25

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 8

agacaaagct cctttaatta gctgc 25

<210> 9

<211> 23

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 9

taaaccctgg agttcctgtc caa 23

<210> 10

<211> 19

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 10

ccagatgcct agggcaagc 19

<210> 11

<211> 21

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 11

gcccaaccta gaggaagttg t 21

<210> 12

<211> 20

<212> DNA

<213> 人工序列(Artificial sequence)

<400> 12

ccatacctga tgccaccctt 20

Claims (4)

1. circRNA生物标志物在制备肺炎诊断试剂中的应用；所述circRNA生物标志物选自如下生物标志物中的一种或几种：

（1）hsa_circ_0018429：序列如SEQ ID NO：1所示；

（2）hsa_circ_0125357：序列如SEQ ID NO：2所示；

（3）hsa_circ_0026579：序列如SEQ ID NO：3所示；

（4）hsa_circ_0099188：序列如SEQ ID NO：4所示。

2.肺炎诊断用circRNA引物在制备肺炎诊断试剂中的应用，其特征在于，所述肺炎诊断用circRNA引物选自如下引物组中的一种或几种：

（1）用于扩增hsa_circ_0018429的引物组；所述用于扩增hsa_circ_0018429的引物组由SEQ ID NO：5所示正向引物和SEQ ID NO：6所示反向引物组成；

（2）用于扩增hsa_circ_0125357的引物组；所述用于扩增hsa_circ_0125357的引物组由SEQ ID NO：7所示正向引物和SEQ ID NO：8所示反向引物组成；

（3）用于扩增hsa_circ_0026579的引物组；所述用于扩增hsa_circ_0026579的引物组由SEQ ID NO：9所示正向引物和SEQ ID NO：10所示反向引物组成；

（4）用于扩增hsa_circ_0099188的引物组；所述用于扩增hsa_circ_0099188的引物组由SEQ ID NO：11所示正向引物和SEQ ID NO：12所示反向引物组成。

3.根据权利要求2所述的应用，其特征在于，所述肺炎诊断试剂还包括用于PCR扩增的其它试剂。

4.根据权利要求3所述的肺炎诊断试剂，其特征在于，所述用于PCR扩增的其它试剂为彩色荧光定量预混试剂。

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