CN109810876A - A kind of seeding tank connection structure and its application method preventing miscellaneous bacteria cross contamination - Google Patents
A kind of seeding tank connection structure and its application method preventing miscellaneous bacteria cross contamination Download PDFInfo
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- CN109810876A CN109810876A CN201910266525.2A CN201910266525A CN109810876A CN 109810876 A CN109810876 A CN 109810876A CN 201910266525 A CN201910266525 A CN 201910266525A CN 109810876 A CN109810876 A CN 109810876A
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- Prior art keywords
- seeding tank
- exhaust
- valve
- pipe
- seeding
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- 238000010899 nucleation Methods 0.000 title claims abstract description 71
- 241000894006 Bacteria Species 0.000 title claims abstract description 14
- 238000012864 cross contamination Methods 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims description 11
- 238000007600 charging Methods 0.000 claims description 11
- 238000000855 fermentation Methods 0.000 claims description 11
- 230000004151 fermentation Effects 0.000 claims description 11
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 239000007789 gas Substances 0.000 claims description 6
- 238000010926 purge Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 239000000356 contaminant Substances 0.000 claims description 3
- 239000002921 fermentation waste Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 241000235342 Saccharomycetes Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of seeding tank connection structures for preventing miscellaneous bacteria cross contamination, including seeding tank, feeding pipe and exhaust main, feeding pipe and exhaust main are arranged under seeding tank, air compressor machine is connected with exhaust main blow down valve by feeding pipe blow down valve respectively, feeding pipe is connect at the top of seeding tank by feed pipe, feed pipe and seeding tank junction are equipped with inlet valve, and exhaust main is connect at the top of seeding tank by exhaust pipe, and exhaust pipe and the seeding tank junction are equipped with inlet valve.Feeding pipe and exhaust main are arranged under seeding tank by the present invention, can effectively keep away the miscellaneous bacteria cross contamination between pipeline, adjacent tank.
Description
Technical field
The present invention relates to biological fermentation field more particularly to a kind of seeding tank connection structure for preventing miscellaneous bacteria cross contamination and
Its application method.
Background technique
It, also will be together with wherein other than carrying out slack tank dissipation to culture vessel, culture tank before saccharomycete mass production culture
Culture etc. sterilize together, sterilization treatment is carried out to production environment, Work tool and connecting pipe, additional oxygen system etc..
However stringent, but in entire cultivation cycle, also will appear microbiological contamination phenomenon frequently, trace it to its cause: first is that sterilizing is not thorough
Bottom, sterilizing methods are improper, second is that the connection method of association pipeline is not scientific, tank currently in use and stand-by slack tank will be enabled
Situations such as connection of tank is improper, and tank will be sterilized or enable by causing is interfered with each other with using tank, and there are cleanings, sterilizing dead angle.
Summary of the invention
The present invention sterilizes halfway problem for existing saccharomycete culture tank, and providing a kind of prevents miscellaneous bacteria cross contamination
Seeding tank connection structure and its application method.
The technical scheme to solve the above technical problems is that a kind of seeding tank connection for preventing miscellaneous bacteria cross contamination
Structure, including seeding tank, feeding pipe and exhaust main, which is characterized in that the feeding pipe and exhaust main are arranged at
Under the seeding tank, connect air compressor machine with exhaust main blow down valve by feeding pipe blow down valve respectively, the feeding pipe with
It is connected at the top of the seeding tank by feed pipe, the feed pipe and the seeding tank junction are equipped with inlet valve, the exhaust
General pipeline is connect at the top of the seeding tank by exhaust pipe, and the exhaust pipe and the seeding tank junction are equipped with exhaust valve.
Feed pipe and exhaust pipe are arranged each parallel to seeding tank, and top is connect by horizontal transverse tube with tank deck, inlet valve
It is arranged on transverse tube with exhaust valve.Feeding pipe diameter is 63.5mm, and exhaust main diameter is 219mm, feed pipe and exhaust
Pipe diameter is 63.5mm, exhaust pipe with exhaust main be it is tangentially connected, cut-out direction flows to identical with compressed air.
Seeding tank connection structure of the present invention is related at least two seeding tank.
The beneficial effect of seeding tank connection structure of the present invention is: feeding pipe and exhaust main are arranged at kind by the present invention
Under sub- tank and feed pipe and exhaust pipe are vertically arranged, and avoid secondary pollution of the heels to seeding tank, inlet valve in pipeline
The problem of valve body accumulates defective material is vertically arranged with horizontally disposed avoid of exhaust valve；Exhaust pipe with exhaust main is tangentially connected can edge
Tangential to generate jet power, exhaust gas will not be flow backwards in exhaust main, can also generate swabbing action to the exhaust pipe of other seeding tanks.
Seeding tank connection structure of the present invention can effectively keep away the miscellaneous bacteria cross contamination between pipeline, adjacent tank.
The invention also discloses use above-mentioned seeding tank connection structure to carry out the fermentation operation without living contaminants, specific steps
Are as follows:
1) preparation: opening all inlet valve and exhaust valve, carries out sterilization treatment to each seeding tank and pipeline,
All valves are closed after sterilizing；
2) it feeds: determining the seeding tank of charging, first open its exhaust valve and open its inlet valve again, after charging, first close
It closes inlet valve and turns off exhaust valve, ferment；
3) be vented: in fermentation process, interval opens exhaust valve and fermentation waste gas is discharged；
4) discharge: after fermentation, closing inlet valve, open exhaust valve, and the blow-off valve for opening seeding tank lower part will ferment
Object discharge；
5) it purges pipeline: opening feeding pipe blow down valve after charging and feeding pipe is purged, exhaust is opened after exhaust
General pipeline blow down valve purges exhaust main.
When multiple seeding tanks are in working condition, each seeding tank can carry out rapid 2) to step at any time
4) operation.Namely each seeding tank can work independently, and not influenced by other seeding tanks.
Further, in order to accelerate the discharge of exhaust gas, exhaust main blow down valve can be normally opened.
Seeding tank connection structure permission multiple tank of the present invention is fermented simultaneously without generating mutually pollution, easy to operate and effect
Rate is high.
Detailed description of the invention
Fig. 1 is seeding tank connection schematic diagram of the present invention；Fig. 2 is existing seeding tank connection schematic diagram；Each section is respectively as follows:
1, seeding tank, 2, feeding pipe, 3, feeding pipe blow down valve, 4, exhaust main, 5, exhaust main blow down valve, 6, into
Expects pipe, 7, inlet valve, 8, exhaust pipe, 9, exhaust valve.
Specific embodiment
Below in conjunction with example, present invention is described, and the given examples are served only to explain the present invention, is not intended to limit this
The range of invention.
As shown in Figure 1, a kind of seeding tank connection structure for preventing miscellaneous bacteria cross contamination, including seeding tank 1, feeding pipe 2
With exhaust main 4,2 diameter of feeding pipe is 63.5mm, and 4 diameter of exhaust main is 219mm, is arranged under seeding tank 1, point
Not by feeding pipe blow down valve 3 and 4 blow down valve 5 of exhaust main connect air compressor machine, feeding pipe 2 and 1 top of seeding tank by into
Expects pipe 6 connects, and feed pipe 6 and 1 junction of seeding tank are equipped with inlet valve 7, passes through exhaust pipe 8 at the top of exhaust main 4 and seeding tank 1
Connection, exhaust pipe 8 and 1 junction of seeding tank are equipped with exhaust valve 9, and exhaust pipe 8 is tangentially connected, cut-out direction with exhaust main 4
Flowed to compressed air identical, feed pipe 6 and 8 diameter of exhaust pipe are 63.5mm, and inlet valve 7 and exhaust valve 9 are horizontally disposed.
The operating procedure fermented using above-mentioned seeding tank connection structure are as follows: 1) preparation: all chargings are opened
Valve and exhaust valve carry out sterilization treatment to each seeding tank and pipeline, all valves are closed after sterilizing；2) feed: determine into
The seeding tank of material first opens its exhaust valve and opens its inlet valve again, after charging, first closes inlet valve and turns off exhaust valve,
It ferments；3) be vented: in fermentation process, interval opens exhaust valve and fermentation waste gas is discharged；4) it discharge: after fermentation, closes
Inlet valve is closed, exhaust valve is opened, fermentation material is discharged the blow-off valve for opening seeding tank lower part；5) it purges pipeline: being opened after charging
Feeding pipe blow down valve purges feeding pipe, and exhaust main blow down valve is opened after exhaust and is purged to exhaust main.
By taking three seeding tanks work at the same time as an example, compressed air flow direction carries out 1# tank, 2# tank and the sequence of 3# tank respectively, a certain
The state of moment three tanks simultaneously can be with are as follows: 1# tank is being fed, and inlet valve is to open, and exhaust valve is to close；In the fermentation of 2# tank,
Its inlet valve is to close, exhaust valve pause switch；3# ullage tank waits feeding, and inlet valve is closed, and exhaust valve is opened；Exhaust main
Blow down valve can also be lasting opening with 2# tank exhaust valve synchronous switch.
Fig. 2 is existing seeding tank connection structure and difference of the invention is that feeding pipe and exhaust main are arranged in seed
The drawbacks of upper end of tank, inlet valve and exhaust valve are vertically arranged, this structure is inlet valve and the meeting of exhaust valve closure upper back
There are waste material or waste liquid to accumulate, can enter in seeding tank after valve opening and generate living contaminants.Multiple seeding tanks are fed or are vented
There is the phenomenon that mutually pollution afterwards, operates while can not be relatively independent.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of seeding tank connection structure for preventing miscellaneous bacteria cross contamination, including seeding tank, feeding pipe and exhaust main, special
Sign is that the feeding pipe and exhaust main are arranged under the seeding tank, respectively by feeding pipe blow down valve and row
Gas general pipeline blow down valve connects air compressor machine, and the feeding pipe is connect at the top of the seeding tank by feed pipe, the feed pipe
It is equipped with inlet valve with the seeding tank junction, the exhaust main is connect at the top of the seeding tank by exhaust pipe, described
Exhaust pipe and the seeding tank junction are equipped with exhaust valve.
2. the seeding tank connection structure according to claim 1 for preventing miscellaneous bacteria cross contamination, which is characterized in that the charging
Valve and the exhaust valve are horizontally disposed.
3. the seeding tank connection structure according to claim 1 for preventing miscellaneous bacteria cross contamination, which is characterized in that the charging
Total pipe diameter is 63.5mm, and the exhaust main diameter is 219mm, and the feed pipe and air slit diameter are 63.5mm.
4. the seeding tank connection structure according to claim 3 for preventing miscellaneous bacteria cross contamination, which is characterized in that the exhaust
It is tangentially connected for managing with the exhaust main, and cut-out direction flows to identical with compressed air.
5. the seeding tank connection structure according to claim 1-4 for preventing miscellaneous bacteria cross contamination, which is characterized in that
Described seeding tank at least two.
6. the seeding tank application method that one kind can prevent living contaminants, which is characterized in that the seeding tank has such as claim 5
The connection structure, application method are as follows:
1) preparation: opening all inlet valve and exhaust valve, carries out sterilization treatment to each seeding tank and pipeline, sterilizing
After close all valves；
2) feed: determine charging seeding tank, first open its exhaust valve and open its inlet valve again, after charging, first close into
Material valve turns off exhaust valve, ferments；
3) be vented: in fermentation process, interval opens exhaust valve and fermentation waste gas is discharged；
4) discharge: after fermentation, inlet valve is closed, opens exhaust valve, the blow-off valve for opening seeding tank lower part arranges fermentation material
Out；
5) it purges pipeline: opening feeding pipe blow down valve after charging and feeding pipe is purged, exhaust main is opened after exhaust
Blow down valve purges exhaust main.
7. according to the method described in claim 6, it is characterized in that, when multiple seeding tanks are in working condition, each kind
Sub- tank can all carry out the rapid operation for 2) arriving step 4) at any time.
8. method according to claim 6 or 7, which is characterized in that exhaust main blow down valve can be normally opened.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910164682.2A CN109679829A (en) | 2019-03-05 | 2019-03-05 | A kind of seeding tank connection structure and its application method preventing miscellaneous bacteria cross contamination |
| CN2019101646822 | 2019-03-05 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109810876A true CN109810876A (en) | 2019-05-28 |
| CN109810876B CN109810876B (en) | 2024-01-09 |
Family
ID=66197621
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910164682.2A Withdrawn CN109679829A (en) | 2019-03-05 | 2019-03-05 | A kind of seeding tank connection structure and its application method preventing miscellaneous bacteria cross contamination |
| CN201910266525.2A Active CN109810876B (en) | 2019-03-05 | 2019-04-03 | Seed tank connecting structure for preventing cross contamination of mixed bacteria and application method thereof |
| CN201920446340.5U Active CN209702735U (en) | 2019-03-05 | 2019-04-03 | A kind of seeding tank connection structure preventing miscellaneous bacteria cross contamination |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910164682.2A Withdrawn CN109679829A (en) | 2019-03-05 | 2019-03-05 | A kind of seeding tank connection structure and its application method preventing miscellaneous bacteria cross contamination |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920446340.5U Active CN209702735U (en) | 2019-03-05 | 2019-04-03 | A kind of seeding tank connection structure preventing miscellaneous bacteria cross contamination |
Country Status (1)
| Country | Link |
|---|---|
| CN (3) | CN109679829A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109679829A (en) * | 2019-03-05 | 2019-04-26 | 山东鲁花生物科技有限公司 | A kind of seeding tank connection structure and its application method preventing miscellaneous bacteria cross contamination |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4945048A (en) * | 1987-05-23 | 1990-07-31 | Takeda Chemical Industries, Ltd. | Process for producing L-sorbose by subculture of seed |
| EP2251408A1 (en) * | 2009-05-11 | 2010-11-17 | KOMPOFERM GmbH | Method and device for operating a fermentation assembly |
| US20120122175A1 (en) * | 2010-11-17 | 2012-05-17 | Kompoferm Gmbh | Method and device for operating a fermentation plant |
| US20130078700A1 (en) * | 2010-05-06 | 2013-03-28 | Gicon Grossmann Ingenieur Consult Gmbh | Method and system for the gas-tight process control of percolators in a biogas method having two or more stages |
| CN104293647A (en) * | 2014-09-19 | 2015-01-21 | 北京利晟新能生物科技中心（有限合伙） | Fermentation equipment and fermentation process for continuous fermentation or intermittent fermentation |
| CN105820950A (en) * | 2016-05-16 | 2016-08-03 | 湖北广济药业股份有限公司 | Unicellular microorganism fermentation system and method |
| CN206109401U (en) * | 2016-10-10 | 2017-04-19 | 济南诺能生物工程有限公司 | Fermentation cylinder composite set |
| CN207784202U (en) * | 2017-05-27 | 2018-08-31 | 福建省古田县程久红粬有限公司 | A kind of red koji fermentation equipment |
| CN209702735U (en) * | 2019-03-05 | 2019-11-29 | 山东鲁花生物科技有限公司 | A kind of seeding tank connection structure preventing miscellaneous bacteria cross contamination |
-
2019
- 2019-03-05 CN CN201910164682.2A patent/CN109679829A/en not_active Withdrawn
- 2019-04-03 CN CN201910266525.2A patent/CN109810876B/en active Active
- 2019-04-03 CN CN201920446340.5U patent/CN209702735U/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4945048A (en) * | 1987-05-23 | 1990-07-31 | Takeda Chemical Industries, Ltd. | Process for producing L-sorbose by subculture of seed |
| EP2251408A1 (en) * | 2009-05-11 | 2010-11-17 | KOMPOFERM GmbH | Method and device for operating a fermentation assembly |
| US20130078700A1 (en) * | 2010-05-06 | 2013-03-28 | Gicon Grossmann Ingenieur Consult Gmbh | Method and system for the gas-tight process control of percolators in a biogas method having two or more stages |
| US20120122175A1 (en) * | 2010-11-17 | 2012-05-17 | Kompoferm Gmbh | Method and device for operating a fermentation plant |
| CN104293647A (en) * | 2014-09-19 | 2015-01-21 | 北京利晟新能生物科技中心（有限合伙） | Fermentation equipment and fermentation process for continuous fermentation or intermittent fermentation |
| CN105820950A (en) * | 2016-05-16 | 2016-08-03 | 湖北广济药业股份有限公司 | Unicellular microorganism fermentation system and method |
| CN206109401U (en) * | 2016-10-10 | 2017-04-19 | 济南诺能生物工程有限公司 | Fermentation cylinder composite set |
| CN207784202U (en) * | 2017-05-27 | 2018-08-31 | 福建省古田县程久红粬有限公司 | A kind of red koji fermentation equipment |
| CN209702735U (en) * | 2019-03-05 | 2019-11-29 | 山东鲁花生物科技有限公司 | A kind of seeding tank connection structure preventing miscellaneous bacteria cross contamination |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109679829A (en) | 2019-04-26 |
| CN109810876B (en) | 2024-01-09 |
| CN209702735U (en) | 2019-11-29 |
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