CN109804087A - 使用猝灭剂的基于光学的纳米孔测序 - Google Patents

使用猝灭剂的基于光学的纳米孔测序 Download PDF

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Publication number
CN109804087A
CN109804087A CN201780062870.3A CN201780062870A CN109804087A CN 109804087 A CN109804087 A CN 109804087A CN 201780062870 A CN201780062870 A CN 201780062870A CN 109804087 A CN109804087 A CN 109804087A
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CN
China
Prior art keywords
nano
polynucleotides
pore
nucleotide
fluorescent
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CN201780062870.3A
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English (en)
Chinese (zh)
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斯蒂芬·C·梅斯维兹
史蒂文·孟肯
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Quantapore Inc
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Quantapore Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201780062870.3A 2016-08-19 2017-07-25 使用猝灭剂的基于光学的纳米孔测序 Pending CN109804087A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662377409P 2016-08-19 2016-08-19
US62/377,409 2016-08-19
PCT/US2017/043772 WO2018034807A1 (fr) 2016-08-19 2017-07-25 Séquençage par nanopores à base optique à l'aide d'agents d'extinction

Publications (1)

Publication Number Publication Date
CN109804087A true CN109804087A (zh) 2019-05-24

Family

ID=61196974

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780062870.3A Pending CN109804087A (zh) 2016-08-19 2017-07-25 使用猝灭剂的基于光学的纳米孔测序

Country Status (6)

Country Link
EP (1) EP3500684A4 (fr)
JP (1) JP2019528065A (fr)
CN (1) CN109804087A (fr)
AU (1) AU2017312746A1 (fr)
CA (1) CA3031812A1 (fr)
WO (1) WO2018034807A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114286867A (zh) * 2019-08-20 2022-04-05 深圳华大智造极创科技有限公司 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019528065A (ja) * 2016-08-19 2019-10-10 クアンタポール， インコーポレイテッド 消光剤を使用する光学に基づくナノポア配列決定
WO2022204442A1 (fr) * 2021-03-24 2022-09-29 Northeastern University Procédé et système de décodage d'informations stockées sur une séquence polymère

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050095599A1 (en) * 2003-10-30 2005-05-05 Pittaro Richard J. Detection and identification of biopolymers using fluorescence quenching
US20120270221A1 (en) * 2006-05-12 2012-10-25 Pronucleotein Biotechnologies, Llc Methods of sequencing fluorophore-quencher FRET-aptamers
US20130203050A1 (en) * 2009-09-30 2013-08-08 Quantapore, Inc. Hybrid nanopore device with optical detection and methods of using same
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114286867A (zh) * 2019-08-20 2022-04-05 深圳华大智造极创科技有限公司 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法
CN114286867B (zh) * 2019-08-20 2024-04-16 青岛华大智造科技有限责任公司 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法

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Publication number Publication date
EP3500684A4 (fr) 2020-02-26
AU2017312746A1 (en) 2019-01-31
WO2018034807A1 (fr) 2018-02-22
JP2019528065A (ja) 2019-10-10
EP3500684A1 (fr) 2019-06-26
CA3031812A1 (fr) 2018-02-22

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