CA3031812A1 - Sequencage par nanopores a base optique a l'aide d'agents d'extinction - Google Patents
Sequencage par nanopores a base optique a l'aide d'agents d'extinction Download PDFInfo
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- CA3031812A1 CA3031812A1 CA3031812A CA3031812A CA3031812A1 CA 3031812 A1 CA3031812 A1 CA 3031812A1 CA 3031812 A CA3031812 A CA 3031812A CA 3031812 A CA3031812 A CA 3031812A CA 3031812 A1 CA3031812 A1 CA 3031812A1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Abstract
La présente invention concerne des procédés et des compositions pour l'analyse de polynucléotides à base optique à l'aide de nanopores, des agents d'extinction étant utilisés pour réduire ou éliminer des signaux optiques parasites générés par des marqueurs polynucléotidiques à l'extérieur de zones de détection. Dans certains modes de réalisation, l'invention peut être mise en oeuvre par les étapes suivantes, consistant à : effectuer une translocation d'un polynucléotide à travers un nanopore, différents types de nucléotides du polynucléotide étant marqués par différentes étiquettes fluorescentes qui génèrent des signaux fluorescents pouvant être distingués et le nanopore contraignant les nucléotides à se déplacer en une file unique à travers une zone de détection ; exciter les marqueurs fluorescents ; éteindre des signaux fluorescents provenant d'étiquettes fluorescentes excitées à l'extérieur de la zone de détection à l'aide d'un ou plusieurs agents d'extinction non fluorescents ; détecter des signaux fluorescents émis par les marqueurs fluorescents lorsque les marqueurs fluorescents passent à travers la zone de détection ; et déterminer une séquence nucléotidique à partir des signaux fluorescents détectés.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662377409P | 2016-08-19 | 2016-08-19 | |
| US62/377,409 | 2016-08-19 | ||
| PCT/US2017/043772 WO2018034807A1 (fr) | 2016-08-19 | 2017-07-25 | Séquençage par nanopores à base optique à l'aide d'agents d'extinction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3031812A1 true CA3031812A1 (fr) | 2018-02-22 |
Family
ID=61196974
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3031812A Abandoned CA3031812A1 (fr) | 2016-08-19 | 2017-07-25 | Sequencage par nanopores a base optique a l'aide d'agents d'extinction |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP3500684A4 (fr) |
| JP (1) | JP2019528065A (fr) |
| CN (1) | CN109804087A (fr) |
| AU (1) | AU2017312746A1 (fr) |
| CA (1) | CA3031812A1 (fr) |
| WO (1) | WO2018034807A1 (fr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3031812A1 (fr) * | 2016-08-19 | 2018-02-22 | Quantapore, Inc. | Sequencage par nanopores a base optique a l'aide d'agents d'extinction |
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-
2017
- 2017-07-25 CA CA3031812A patent/CA3031812A1/fr not_active Abandoned
- 2017-07-25 AU AU2017312746A patent/AU2017312746A1/en not_active Abandoned
- 2017-07-25 WO PCT/US2017/043772 patent/WO2018034807A1/fr unknown
- 2017-07-25 EP EP17841831.5A patent/EP3500684A4/fr not_active Withdrawn
- 2017-07-25 JP JP2019509522A patent/JP2019528065A/ja active Pending
- 2017-07-25 CN CN201780062870.3A patent/CN109804087A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3500684A4 (fr) | 2020-02-26 |
| CN109804087A (zh) | 2019-05-24 |
| EP3500684A1 (fr) | 2019-06-26 |
| AU2017312746A1 (en) | 2019-01-31 |
| JP2019528065A (ja) | 2019-10-10 |
| WO2018034807A1 (fr) | 2018-02-22 |
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