CN109803978A - 多肽及其应用 - Google Patents
多肽及其应用 Download PDFInfo
- Publication number
- CN109803978A CN109803978A CN201680088999.7A CN201680088999A CN109803978A CN 109803978 A CN109803978 A CN 109803978A CN 201680088999 A CN201680088999 A CN 201680088999A CN 109803978 A CN109803978 A CN 109803978A
- Authority
- CN
- China
- Prior art keywords
- polypeptide
- cell
- cancer
- tumour
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 414
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 410
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 404
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 176
- 210000004027 cell Anatomy 0.000 claims abstract description 171
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 80
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 76
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 76
- 229960005486 vaccine Drugs 0.000 claims abstract description 46
- 210000000612 antigen-presenting cell Anatomy 0.000 claims abstract description 41
- 239000013604 expression vector Substances 0.000 claims abstract description 37
- 238000011282 treatment Methods 0.000 claims abstract description 32
- 239000012642 immune effector Substances 0.000 claims abstract description 26
- 229940121354 immunomodulator Drugs 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 22
- 239000008194 pharmaceutical composition Substances 0.000 claims abstract description 21
- 238000002405 diagnostic procedure Methods 0.000 claims abstract description 12
- 108010088729 HLA-A*02:01 antigen Proteins 0.000 claims description 72
- 150000001413 amino acids Chemical class 0.000 claims description 34
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 33
- 206010058467 Lung neoplasm malignant Diseases 0.000 claims description 22
- 201000005202 lung cancer Diseases 0.000 claims description 22
- 208000020816 lung neoplasm Diseases 0.000 claims description 22
- 208000003174 Brain Neoplasms Diseases 0.000 claims description 20
- 206010006187 Breast cancer Diseases 0.000 claims description 20
- 208000026310 Breast neoplasm Diseases 0.000 claims description 20
- 201000009030 Carcinoma Diseases 0.000 claims description 20
- 208000017897 Carcinoma of esophagus Diseases 0.000 claims description 20
- 208000000461 Esophageal Neoplasms Diseases 0.000 claims description 20
- 208000002454 Nasopharyngeal Carcinoma Diseases 0.000 claims description 20
- 206010061306 Nasopharyngeal cancer Diseases 0.000 claims description 20
- 206010061902 Pancreatic neoplasm Diseases 0.000 claims description 20
- 206010060862 Prostate cancer Diseases 0.000 claims description 20
- 208000000236 Prostatic Neoplasms Diseases 0.000 claims description 20
- 208000005718 Stomach Neoplasms Diseases 0.000 claims description 20
- 208000019065 cervical carcinoma Diseases 0.000 claims description 20
- 206010017758 gastric cancer Diseases 0.000 claims description 20
- 208000032839 leukemia Diseases 0.000 claims description 20
- 201000007270 liver cancer Diseases 0.000 claims description 20
- 208000014018 liver neoplasm Diseases 0.000 claims description 20
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 claims description 20
- 201000001441 melanoma Diseases 0.000 claims description 20
- 201000011216 nasopharynx carcinoma Diseases 0.000 claims description 20
- 201000011549 stomach cancer Diseases 0.000 claims description 20
- 238000001514 detection method Methods 0.000 claims description 19
- 206010009944 Colon cancer Diseases 0.000 claims description 18
- 208000001333 Colorectal Neoplasms Diseases 0.000 claims description 18
- 239000002671 adjuvant Substances 0.000 claims description 17
- 239000003814 drug Substances 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 14
- 238000006467 substitution reaction Methods 0.000 claims description 14
- 229940079593 drug Drugs 0.000 claims description 13
- 239000012472 biological sample Substances 0.000 claims description 12
- 230000001900 immune effect Effects 0.000 claims description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims description 11
- 201000010099 disease Diseases 0.000 claims description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 11
- 101150032176 COL21A1 gene Proteins 0.000 claims description 10
- 238000012217 deletion Methods 0.000 claims description 9
- 230000037430 deletion Effects 0.000 claims description 9
- 206010064571 Gene mutation Diseases 0.000 claims description 8
- 230000030741 antigen processing and presentation Effects 0.000 claims description 8
- 210000002966 serum Anatomy 0.000 claims description 7
- 238000011081 inoculation Methods 0.000 claims description 6
- 230000000295 complement effect Effects 0.000 claims description 5
- 210000004443 dendritic cell Anatomy 0.000 claims description 5
- 230000036039 immunity Effects 0.000 claims description 4
- 241001465754 Metazoa Species 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 4
- 208000005016 Intestinal Neoplasms Diseases 0.000 claims 2
- 201000002313 intestinal cancer Diseases 0.000 claims 2
- 229940030156 cell vaccine Drugs 0.000 abstract description 4
- 238000007689 inspection Methods 0.000 abstract description 2
- 230000014509 gene expression Effects 0.000 description 34
- 230000000694 effects Effects 0.000 description 30
- 241000699666 Mus <mouse, genus> Species 0.000 description 26
- 230000002018 overexpression Effects 0.000 description 22
- 210000001519 tissue Anatomy 0.000 description 20
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 19
- 230000028993 immune response Effects 0.000 description 18
- 230000002147 killing effect Effects 0.000 description 18
- 201000011510 cancer Diseases 0.000 description 13
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 13
- 230000002265 prevention Effects 0.000 description 12
- 210000004881 tumor cell Anatomy 0.000 description 12
- 241000282414 Homo sapiens Species 0.000 description 11
- 108091028043 Nucleic acid sequence Proteins 0.000 description 11
- 239000000427 antigen Substances 0.000 description 11
- 102000036639 antigens Human genes 0.000 description 11
- 108091007433 antigens Proteins 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 11
- 238000002649 immunization Methods 0.000 description 11
- 230000035772 mutation Effects 0.000 description 11
- 238000002560 therapeutic procedure Methods 0.000 description 11
- 230000004913 activation Effects 0.000 description 10
- 230000006870 function Effects 0.000 description 10
- 230000003053 immunization Effects 0.000 description 10
- 108010074328 Interferon-gamma Proteins 0.000 description 9
- 241000700605 Viruses Species 0.000 description 9
- 239000007924 injection Substances 0.000 description 9
- 238000002347 injection Methods 0.000 description 9
- 230000004083 survival effect Effects 0.000 description 9
- 230000001225 therapeutic effect Effects 0.000 description 9
- 102100037850 Interferon gamma Human genes 0.000 description 8
- 238000003745 diagnosis Methods 0.000 description 8
- 230000007969 cellular immunity Effects 0.000 description 7
- 230000008859 change Effects 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000003114 enzyme-linked immunosorbent spot assay Methods 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- -1 host cell Substances 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 239000012636 effector Substances 0.000 description 5
- 229940044627 gamma-interferon Drugs 0.000 description 5
- 238000009169 immunotherapy Methods 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 230000004614 tumor growth Effects 0.000 description 5
- 238000012795 verification Methods 0.000 description 5
- 229920001917 Ficoll Polymers 0.000 description 4
- 206010041047 Slow virus infection Diseases 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 4
- 229940029030 dendritic cell vaccine Drugs 0.000 description 4
- 230000006698 induction Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 210000005259 peripheral blood Anatomy 0.000 description 4
- 239000011886 peripheral blood Substances 0.000 description 4
- RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical compound C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 description 4
- 230000002269 spontaneous effect Effects 0.000 description 4
- 238000007920 subcutaneous administration Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000001890 transfection Methods 0.000 description 4
- 210000003171 tumor-infiltrating lymphocyte Anatomy 0.000 description 4
- 208000010507 Adenocarcinoma of Lung Diseases 0.000 description 3
- 108010029697 CD40 Ligand Proteins 0.000 description 3
- 102100032937 CD40 ligand Human genes 0.000 description 3
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 3
- 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 3
- 108010002350 Interleukin-2 Proteins 0.000 description 3
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 238000011789 NOD SCID mouse Methods 0.000 description 3
- 238000011579 SCID mouse model Methods 0.000 description 3
- 230000001464 adherent effect Effects 0.000 description 3
- 230000010100 anticoagulation Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000035800 maturation Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 210000001616 monocyte Anatomy 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 102000014914 Carrier Proteins Human genes 0.000 description 2
- 108010078791 Carrier Proteins Proteins 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004388 Interleukin-4 Human genes 0.000 description 2
- 108090000978 Interleukin-4 Proteins 0.000 description 2
- 108010002586 Interleukin-7 Proteins 0.000 description 2
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 2
- 206010027476 Metastases Diseases 0.000 description 2
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 2
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 2
- 238000009098 adjuvant therapy Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000002512 chemotherapy Methods 0.000 description 2
- 238000003501 co-culture Methods 0.000 description 2
- 239000003636 conditioned culture medium Substances 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000012645 endogenous antigen Substances 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 210000004013 groin Anatomy 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 230000005847 immunogenicity Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 201000005249 lung adenocarcinoma Diseases 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229950006238 nadide Drugs 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 229950010131 puromycin Drugs 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 238000001959 radiotherapy Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000001875 tumorinhibitory effect Effects 0.000 description 2
- 238000010200 validation analysis Methods 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 230000003442 weekly effect Effects 0.000 description 2
- UZOVYGYOLBIAJR-UHFFFAOYSA-N 4-isocyanato-4'-methyldiphenylmethane Chemical compound C1=CC(C)=CC=C1CC1=CC=C(N=C=O)C=C1 UZOVYGYOLBIAJR-UHFFFAOYSA-N 0.000 description 1
- 102000002734 Collagen Type VI Human genes 0.000 description 1
- 108010043741 Collagen Type VI Proteins 0.000 description 1
- 102100040995 Collagen alpha-1(XXI) chain Human genes 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 241000702421 Dependoparvovirus Species 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102100028972 HLA class I histocompatibility antigen, A alpha chain Human genes 0.000 description 1
- 108010075704 HLA-A Antigens Proteins 0.000 description 1
- 102210042925 HLA-A*02:01 Human genes 0.000 description 1
- 108010074032 HLA-A2 Antigen Proteins 0.000 description 1
- 102000025850 HLA-A2 Antigen Human genes 0.000 description 1
- 101000748976 Homo sapiens Collagen alpha-1(XXI) chain Proteins 0.000 description 1
- 101001002709 Homo sapiens Interleukin-4 Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 244000283207 Indigofera tinctoria Species 0.000 description 1
- 102000008070 Interferon-gamma Human genes 0.000 description 1
- 108010020437 Ki-67 Antigen Proteins 0.000 description 1
- 102000007651 Macrophage Colony-Stimulating Factor Human genes 0.000 description 1
- 108010046938 Macrophage Colony-Stimulating Factor Proteins 0.000 description 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-O NAD(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-O 0.000 description 1
- 108700001237 Nucleic Acid-Based Vaccines Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 108010071384 Peptide T Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 102100034836 Proliferation marker protein Ki-67 Human genes 0.000 description 1
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- GHWKXZFQRZLLDE-UHFFFAOYSA-O [N+](=O)([O-])C=1N=NN[N+]1Cl Chemical compound [N+](=O)([O-])C=1N=NN[N+]1Cl GHWKXZFQRZLLDE-UHFFFAOYSA-O 0.000 description 1
- MOFINMJRLYEONQ-UHFFFAOYSA-N [N].C=1C=CNC=1 Chemical class [N].C=1C=CNC=1 MOFINMJRLYEONQ-UHFFFAOYSA-N 0.000 description 1
- 230000003302 anti-idiotype Effects 0.000 description 1
- 230000005809 anti-tumor immunity Effects 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 102000015736 beta 2-Microglobulin Human genes 0.000 description 1
- 108010081355 beta 2-Microglobulin Proteins 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000001815 biotherapy Methods 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000020411 cell activation Effects 0.000 description 1
- 230000022534 cell killing Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000004940 costimulation Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- VAYGXNSJCAHWJZ-UHFFFAOYSA-N dimethyl sulfate Chemical compound COS(=O)(=O)OC VAYGXNSJCAHWJZ-UHFFFAOYSA-N 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000012202 endocytosis Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000002607 hemopoietic effect Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 102000055229 human IL4 Human genes 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000003928 nasal cavity Anatomy 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 229940107700 pyruvic acid Drugs 0.000 description 1
- 229940124551 recombinant vaccine Drugs 0.000 description 1
- 210000000664 rectum Anatomy 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000004007 reversed phase HPLC Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 238000002626 targeted therapy Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 229940030325 tumor cell vaccine Drugs 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/08—Peptides having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/0005—Vertebrate antigens
- A61K39/0011—Cancer antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/461—Cellular immunotherapy characterised by the cell type used
- A61K39/4611—T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/461—Cellular immunotherapy characterised by the cell type used
- A61K39/4615—Dendritic cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/462—Cellular immunotherapy characterized by the effect or the function of the cells
- A61K39/4622—Antigen presenting cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
- A61K39/4643—Vertebrate antigens
- A61K39/4644—Cancer antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/545—Medicinal preparations containing antigens or antibodies characterised by the dose, timing or administration schedule
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/57—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2
- A61K2039/572—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2 cytotoxic response
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/80—Vaccine for a specifically defined cancer
- A61K2039/86—Lung
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K39/46
- A61K2239/31—Indexing codes associated with cellular immunotherapy of group A61K39/46 characterized by the route of administration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K39/46
- A61K2239/38—Indexing codes associated with cellular immunotherapy of group A61K39/46 characterised by the dose, timing or administration schedule
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Biochemistry (AREA)
- Oncology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Hospice & Palliative Care (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
一种多肽,编码所述多肽的核酸、包含上述核酸的核酸构建体、表达载体、宿主细胞,在细胞表面呈递上述多肽的抗原呈递细胞及其免疫效应细胞,包含上述多肽的药物组合物,包含上述核酸、核酸构建体、表达载体、宿主细胞、抗原呈递细胞、免疫效应细胞的疫苗,识别上述多肽的抗体。施用上述多肽、核酸、药物组合物、疫苗、抗体的治疗方法。检测上述多肽的诊断方法和诊断装置。上述多肽在用于制备疫苗、诊断肿瘤试剂盒或药物组合物中的用途,以及上述多肽、核酸作为检查靶标在肿瘤诊断中的用途。
Description
PCT国内申请,说明书已公开。
Claims (20)
- PCT国内申请,权利要求书已公开。
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2016/098328 WO2018045509A1 (zh) | 2016-09-07 | 2016-09-07 | 多肽及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109803978A true CN109803978A (zh) | 2019-05-24 |
CN109803978B CN109803978B (zh) | 2022-07-01 |
Family
ID=61561273
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201680088999.7A Active CN109803978B (zh) | 2016-09-07 | 2016-09-07 | 多肽及其应用 |
Country Status (3)
Country | Link |
---|---|
US (1) | US11213563B2 (zh) |
CN (1) | CN109803978B (zh) |
WO (1) | WO2018045509A1 (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115785209A (zh) * | 2022-06-10 | 2023-03-14 | 河北博海生物工程开发有限公司 | 肺癌特异性分子靶标06及其用途 |
CN115785207A (zh) * | 2022-06-10 | 2023-03-14 | 河北博海生物工程开发有限公司 | 肺癌特异性分子靶标02及其用途 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110139875B (zh) * | 2016-09-30 | 2023-03-03 | 武汉华大吉诺因生物科技有限公司 | Col14a1衍生的肿瘤抗原多肽及其应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010086389A1 (en) * | 2009-01-28 | 2010-08-05 | Ait Austrian Institute Of Technology Gmbh | Methylation assay |
CN101998964A (zh) * | 2008-02-15 | 2011-03-30 | 太平洋艾瑞有限公司 | 阻止癌细胞的转移的新化合物及其应用 |
CN102156164A (zh) * | 2011-01-13 | 2011-08-17 | 北京肿瘤医院 | 核仁磷酸蛋白可变剪接体的质谱鉴定方法和胃癌诊断试剂盒 |
CN105247075A (zh) * | 2013-03-15 | 2016-01-13 | 维拉赛特股份有限公司 | 用于诊断肺病的生物标记物及其使用方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7928190B2 (en) | 2007-07-05 | 2011-04-19 | Darnell Robert B | Methods and compositions for tumor vaccination and therapy |
US8389929B2 (en) * | 2010-03-02 | 2013-03-05 | Thermo Finnigan Llc | Quadrupole mass spectrometer with enhanced sensitivity and mass resolving power |
CN102618498B (zh) | 2012-03-26 | 2014-04-09 | 时宏珍 | Hla-a0201限制性抗原特异性ctl制备方法 |
CN105524984B (zh) * | 2014-09-30 | 2021-04-13 | 深圳华大基因科技有限公司 | 预测新抗原表位的方法及设备 |
KR20200116917A (ko) * | 2017-12-20 | 2020-10-13 | 포세이다 테라퓨틱스, 인크. | Vcar 조성물 및 사용 방법 |
-
2016
- 2016-09-07 WO PCT/CN2016/098328 patent/WO2018045509A1/zh active Application Filing
- 2016-09-07 CN CN201680088999.7A patent/CN109803978B/zh active Active
-
2019
- 2019-03-07 US US16/295,653 patent/US11213563B2/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101998964A (zh) * | 2008-02-15 | 2011-03-30 | 太平洋艾瑞有限公司 | 阻止癌细胞的转移的新化合物及其应用 |
WO2010086389A1 (en) * | 2009-01-28 | 2010-08-05 | Ait Austrian Institute Of Technology Gmbh | Methylation assay |
CN102156164A (zh) * | 2011-01-13 | 2011-08-17 | 北京肿瘤医院 | 核仁磷酸蛋白可变剪接体的质谱鉴定方法和胃癌诊断试剂盒 |
CN105247075A (zh) * | 2013-03-15 | 2016-01-13 | 维拉赛特股份有限公司 | 用于诊断肺病的生物标记物及其使用方法 |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115785209A (zh) * | 2022-06-10 | 2023-03-14 | 河北博海生物工程开发有限公司 | 肺癌特异性分子靶标06及其用途 |
CN115785207A (zh) * | 2022-06-10 | 2023-03-14 | 河北博海生物工程开发有限公司 | 肺癌特异性分子靶标02及其用途 |
Also Published As
Publication number | Publication date |
---|---|
US11213563B2 (en) | 2022-01-04 |
WO2018045509A1 (zh) | 2018-03-15 |
US20190263864A1 (en) | 2019-08-29 |
CN109803978B (zh) | 2022-07-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103570818B (zh) | 肿瘤抗原性多肽及其作为肿瘤疫苗的用途 | |
CN110214144A (zh) | 多肽及其应用 | |
US11213563B2 (en) | Polypeptide and use thereof | |
US11466053B2 (en) | Polypeptide and use thereof | |
US11142547B2 (en) | Polypeptide and use thereof | |
CN110167956A (zh) | 多肽及其应用 | |
US11820836B2 (en) | Polypeptide and use thereof | |
CN109311955A (zh) | 一种新的肿瘤特异性多肽及其应用 | |
CN103570821A (zh) | 粘蛋白-1抗原性多肽及其作为肿瘤疫苗的用途 | |
CN109790224A (zh) | Cacna1h衍生的肿瘤抗原多肽及其应用 | |
CN109952308A (zh) | 多肽及其应用 | |
CN110072876A (zh) | 多肽及其应用 | |
CN110139875B (zh) | Col14a1衍生的肿瘤抗原多肽及其应用 | |
CN109414023A (zh) | 用嵌合脊髓灰质炎病毒激活抗原呈递细胞的组合物和方法 | |
CN116350758A (zh) | 肿瘤共享新抗原表位肽或其编码核酸在制备药物中的应用 | |
CN116350760A (zh) | 新抗原esr1来源的ctl表位肽或其编码核酸在制备药物中的应用 | |
CN109748974A (zh) | 一种基因修饰的树突状细胞疫苗的制备及应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 40002414 Country of ref document: HK |
|
GR01 | Patent grant | ||
GR01 | Patent grant |