CN109790510B - 在不存在诱导底物下丝状真菌细胞中的蛋白产生 - Google Patents

在不存在诱导底物下丝状真菌细胞中的蛋白产生 Download PDF

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CN109790510B
CN109790510B CN201780059992.7A CN201780059992A CN109790510B CN 109790510 B CN109790510 B CN 109790510B CN 201780059992 A CN201780059992 A CN 201780059992A CN 109790510 B CN109790510 B CN 109790510B
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seq
ace3
protein
promoter
nucleic acid
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CN109790510A (zh
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M·华德
Y·罗
F·O·本德苏
M·瓦尔科宁
M·萨罗海摩
N·阿罗
T·帕库拉
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Vtt Technical Res Centre Of Finland Ltd
Danisco US Inc
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Danisco US Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201780059992.7A 2016-10-04 2017-10-03 在不存在诱导底物下丝状真菌细胞中的蛋白产生 Active CN109790510B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662403787P 2016-10-04 2016-10-04
US62/403,787 2016-10-04
PCT/US2017/054983 WO2018067599A1 (en) 2016-10-04 2017-10-03 Protein production in filamentous fungal cells in the absence of inducing substrates

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CN109790510B true CN109790510B (zh) 2024-04-12

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EP (1) EP3523415A1 (enExample)
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US20220025423A1 (en) * 2018-11-28 2022-01-27 Novozymes A/S Modified Filamentous Fungal Host Cells
US12534500B2 (en) 2019-07-16 2026-01-27 Centro Nacional De Pesquisa Em Energia E Materiais Modified Trichoderma fungal strain for the production of an enzyme cocktail
EP4055177B1 (en) * 2019-11-08 2024-07-31 Danisco US Inc. Fungal strains comprising enhanced protein productivity phenotypes and methods thereof
US12606837B2 (en) 2019-11-18 2026-04-21 Kao Corporation Mutant filamentous fungus, and method for producing protein using same
CN113106114B (zh) * 2020-01-13 2024-04-12 中国科学院分子植物科学卓越创新中心 调控里氏木霉蛋白表达效率的因子、调控方法及应用
JP7799621B2 (ja) * 2020-04-22 2026-01-15 ダニスコ・ユーエス・インク 糸状菌細胞におけるタンパク質産生の増強のための組成物及び方法
JP7827410B2 (ja) * 2021-04-09 2026-03-10 花王株式会社 改変糸状菌、及びそれを用いたタンパク質の製造方法
CN116813728B (zh) * 2023-07-07 2024-08-09 山东大学 一种真菌木质纤维素降解酶合成调控蛋白突变体及其应用
CN118389304A (zh) * 2023-12-28 2024-07-26 福州道福生物科技有限公司 一种生产索马甜ⅰ蛋白的重组里氏木霉菌株及其构建方法与应用
WO2025165968A1 (en) 2024-01-31 2025-08-07 Danisco Us Inc. Conditional regulation of protein function in filamentous fungal cells
CN121202977B (zh) * 2025-10-28 2026-05-01 华东理工大学 里氏木霉转录激活因子ace3突变体、编码基因及其应用

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JP7285780B2 (ja) 2023-06-02
WO2018067599A1 (en) 2018-04-12
KR102593668B1 (ko) 2023-10-24
US10876103B2 (en) 2020-12-29
US20190309276A1 (en) 2019-10-10
EP3523415A1 (en) 2019-08-14
JP7605902B2 (ja) 2024-12-24
CN109790510A (zh) 2019-05-21
JP2023109919A (ja) 2023-08-08
US20220145278A1 (en) 2022-05-12
JP2019528797A (ja) 2019-10-17
KR20190057088A (ko) 2019-05-27

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