JP7285780B2 - 誘導基質の非存在下における糸状菌細胞内でのタンパク質の産生 - Google Patents

誘導基質の非存在下における糸状菌細胞内でのタンパク質の産生 Download PDF

Info

Publication number
JP7285780B2
JP7285780B2 JP2019539732A JP2019539732A JP7285780B2 JP 7285780 B2 JP7285780 B2 JP 7285780B2 JP 2019539732 A JP2019539732 A JP 2019539732A JP 2019539732 A JP2019539732 A JP 2019539732A JP 7285780 B2 JP7285780 B2 JP 7285780B2
Authority
JP
Japan
Prior art keywords
ace3
seq
protein
promoter
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2019539732A
Other languages
English (en)
Japanese (ja)
Other versions
JP2019528797A5 (enExample
JP2019528797A (ja
Inventor
ウォード、マイケル
ルオ、ユン
オセアス ベンデズ、フェリペ
ヴァルコネン、マリ
サロヘイモ、マルック
アロ、ニナ
パクラ、ティーナ
Original Assignee
ダニスコ・ユーエス・インク
ヴィーティーティー テクニカル リサーチ センター オブ フィンランド リミテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ダニスコ・ユーエス・インク, ヴィーティーティー テクニカル リサーチ センター オブ フィンランド リミテッド filed Critical ダニスコ・ユーエス・インク
Publication of JP2019528797A publication Critical patent/JP2019528797A/ja
Publication of JP2019528797A5 publication Critical patent/JP2019528797A5/ja
Priority to JP2023084937A priority Critical patent/JP7605902B2/ja
Application granted granted Critical
Publication of JP7285780B2 publication Critical patent/JP7285780B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2019539732A 2016-10-04 2017-10-03 誘導基質の非存在下における糸状菌細胞内でのタンパク質の産生 Active JP7285780B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023084937A JP7605902B2 (ja) 2016-10-04 2023-05-23 誘導基質の非存在下における糸状菌細胞内でのタンパク質の産生

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662403787P 2016-10-04 2016-10-04
US62/403,787 2016-10-04
PCT/US2017/054983 WO2018067599A1 (en) 2016-10-04 2017-10-03 Protein production in filamentous fungal cells in the absence of inducing substrates

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023084937A Division JP7605902B2 (ja) 2016-10-04 2023-05-23 誘導基質の非存在下における糸状菌細胞内でのタンパク質の産生

Publications (3)

Publication Number Publication Date
JP2019528797A JP2019528797A (ja) 2019-10-17
JP2019528797A5 JP2019528797A5 (enExample) 2020-11-12
JP7285780B2 true JP7285780B2 (ja) 2023-06-02

Family

ID=60245185

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2019539732A Active JP7285780B2 (ja) 2016-10-04 2017-10-03 誘導基質の非存在下における糸状菌細胞内でのタンパク質の産生
JP2023084937A Active JP7605902B2 (ja) 2016-10-04 2023-05-23 誘導基質の非存在下における糸状菌細胞内でのタンパク質の産生

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2023084937A Active JP7605902B2 (ja) 2016-10-04 2023-05-23 誘導基質の非存在下における糸状菌細胞内でのタンパク質の産生

Country Status (6)

Country Link
US (2) US10876103B2 (enExample)
EP (1) EP3523415A1 (enExample)
JP (2) JP7285780B2 (enExample)
KR (1) KR102593668B1 (enExample)
CN (1) CN109790510B (enExample)
WO (1) WO2018067599A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110760537A (zh) * 2018-07-27 2020-02-07 中国农业科学院饲料研究所 用于快速筛选重组菌株的重组表达载体及应用
US20220025423A1 (en) * 2018-11-28 2022-01-27 Novozymes A/S Modified Filamentous Fungal Host Cells
US12534500B2 (en) 2019-07-16 2026-01-27 Centro Nacional De Pesquisa Em Energia E Materiais Modified Trichoderma fungal strain for the production of an enzyme cocktail
EP4055177B1 (en) * 2019-11-08 2024-07-31 Danisco US Inc. Fungal strains comprising enhanced protein productivity phenotypes and methods thereof
US12606837B2 (en) 2019-11-18 2026-04-21 Kao Corporation Mutant filamentous fungus, and method for producing protein using same
CN113106114B (zh) * 2020-01-13 2024-04-12 中国科学院分子植物科学卓越创新中心 调控里氏木霉蛋白表达效率的因子、调控方法及应用
JP7799621B2 (ja) * 2020-04-22 2026-01-15 ダニスコ・ユーエス・インク 糸状菌細胞におけるタンパク質産生の増強のための組成物及び方法
JP7827410B2 (ja) * 2021-04-09 2026-03-10 花王株式会社 改変糸状菌、及びそれを用いたタンパク質の製造方法
CN116813728B (zh) * 2023-07-07 2024-08-09 山东大学 一种真菌木质纤维素降解酶合成调控蛋白突变体及其应用
CN118389304A (zh) * 2023-12-28 2024-07-26 福州道福生物科技有限公司 一种生产索马甜ⅰ蛋白的重组里氏木霉菌株及其构建方法与应用
WO2025165968A1 (en) 2024-01-31 2025-08-07 Danisco Us Inc. Conditional regulation of protein function in filamentous fungal cells
CN121202977B (zh) * 2025-10-28 2026-05-01 华东理工大学 里氏木霉转录激活因子ace3突变体、编码基因及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007530065A (ja) 2004-04-02 2007-11-01 ディーエスエム アイピー アセッツ ビー．ブイ． 改善された相同的組換え効率を有する糸状菌変異体
WO2011151512A2 (en) 2010-06-04 2011-12-08 Teknologian Tutkimuskeskus Vtt Method for protein production in filamentous fungi
JP2014503185A (ja) 2010-10-20 2014-02-13 ダニスコ・ユーエス・インク 熱安定性トリコデルマ（ｔｒｉｃｈｏｄｅｒｍａ）セルラーゼ

Family Cites Families (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ217373A (en) 1985-08-29 1990-10-26 Genencor Inc Production of heterologous peptide in filamentous fungi, vectors and dna
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
WO1992006221A1 (en) 1990-10-05 1992-04-16 Genencor International, Inc. Methods for treating cotton-containing fabrics with cellulase
EP0551386B1 (en) 1990-10-05 2004-01-07 Genencor International, Inc. Methods for treating cotton-containing fabrics with cellulase
WO1992006183A1 (en) 1990-10-05 1992-04-16 Genencor International, Inc. Methods for treating cotton-containing fabrics with cellulase
DK0562003T4 (en) 1990-12-10 2015-07-13 Danisco Us Inc Improved saccharification of cellulose by cloning and amplification of.-Glucosidase gene from Tricodermareesei
US6017870A (en) 1996-10-09 2000-01-25 Genencor International, Inc. Purified cellulase and method of producing
US6255115B1 (en) 1997-04-07 2001-07-03 Unilever Patent Holdings Bv Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus
US6268328B1 (en) 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
ATE481492T1 (de) 2000-08-04 2010-10-15 Genencor Int Mutierte trichoderma reesei egiii cellulasen, dafür kodierende dna und verfahren zu deren herstellung
US6982159B2 (en) 2001-09-21 2006-01-03 Genencor International, Inc. Trichoderma β-glucosidase
US7049125B2 (en) 2001-12-18 2006-05-23 Genencor International, Inc. EGVIII endoglucanase and nucleic acids encoding the same
US7045331B2 (en) 2001-12-18 2006-05-16 Genencor International, Inc. EGVII endoglucanase and nucleic acids encoding the same
US7045332B2 (en) 2001-12-18 2006-05-16 Genencor International, Inc. BGL4 β-glucosidase and nucleic acids encoding the same
US7005289B2 (en) 2001-12-18 2006-02-28 Genencor International, Inc. BGL5 β-glucosidase and nucleic acids encoding the same
US7056721B2 (en) 2001-12-18 2006-06-06 Genencor International, Inc. EGVI endoglucanase and nucleic acids encoding the same
US7972832B2 (en) 2002-08-16 2011-07-05 Danisco Us Inc. Variant Hypocrea jecorina CBH1 cellulases
JP5366286B2 (ja) 2002-09-10 2013-12-11 ジェネンコー・インターナショナル・インク 高濃度糖類混合物を用いた遺伝子発現の誘発
US7582462B2 (en) 2002-11-07 2009-09-01 Danisco Us Inc., Genencor Division BGL6 beta-glucosidase and nucleic acids encoding the same
US7407788B2 (en) 2002-11-21 2008-08-05 Danisco A/S, Genencor Division BGL7 beta-glucosidase and nucleic acids encoding the same
CN1898381B (zh) 2003-03-21 2013-07-17 金克克国际有限公司 Cbh1同源物和cbh1纤维素酶变体
WO2005001065A2 (en) 2003-04-01 2005-01-06 Genencor International, Inc. Variant humicola grisea cbh1.1
CA2915553A1 (en) 2003-05-29 2005-01-06 Genencor International, Inc. Trichoderma genes
EP1735447A2 (en) 2004-03-25 2006-12-27 Genencor International, Inc. Cellulase fusion protein and heterologous cellulase fusion construct encoding the same
US8097445B2 (en) 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
US7413887B2 (en) 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
EP1838849B1 (en) 2004-12-30 2015-08-26 Danisco US Inc. Variant hypocrea jecorina cbh2 cellulases
US20070231819A1 (en) * 2006-03-15 2007-10-04 Christopher Lawrence Targeted and non-targeted gene insertions using a linear minimal element construct
US8143046B2 (en) 2007-02-07 2012-03-27 Danisco Us Inc., Genencor Division Variant Buttiauxella sp. phytases having altered properties
US20090253173A1 (en) * 2008-04-08 2009-10-08 Danisco Us Inc., Genencor Division Filamentous fungi with inactivated protease genes for altered protein production
EP2628796A1 (en) 2008-06-06 2013-08-21 Danisco US Inc. Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials
CN104862293A (zh) 2009-06-03 2015-08-26 丹尼斯科美国公司 具有改善的表达、活性和/或稳定性的纤维素酶变体,及其用途
CN102791853B (zh) 2009-09-23 2015-08-12 丹尼斯科美国公司 新型糖基水解酶及其用途
CA2780767A1 (en) 2009-11-20 2011-05-26 Danisco Us Inc. Beta-glucosidase i variants with improved properties
CN101831452B (zh) * 2010-04-22 2012-09-05 中国农业科学院生物技术研究所 通过重组瑞氏木霉以诱导型方式高效表达和生产t4溶菌酶的方法
US8962295B2 (en) 2010-06-01 2015-02-24 California Institute Of Technology Stable, functional chimeric cellobiohydrolase class I enzymes
CN102939382B (zh) * 2010-06-03 2017-02-15 丹尼斯科美国公司 丝状真菌宿主菌株和dna构建体以及它们的使用方法
AU2012229042B2 (en) 2011-03-17 2017-03-02 Danisco Us Inc. Glycosyl hydrolase enzymes and uses thereof for biomass hydrolysis
BR112013023715A2 (pt) 2011-03-17 2016-09-13 Danisco Us Inc "polipeptídeo, polinucleotídeo, vetor, célula hospedeira, e composição para uso em hidrólise de biomassa, bem como métodos para hidrolisar material de biomassa celulósica e para produzir composição de mistura de cultura ou caldo de fermentação"
US20140134677A1 (en) 2011-03-17 2014-05-15 Danisco Us Inc. Method for reducing viscosity in saccharification process
CN102311951B (zh) * 2011-07-13 2013-03-20 深圳大学 里氏木霉组成型表达盒、表达载体、重组菌株及其应用
WO2013096056A1 (en) 2011-12-21 2013-06-27 Danisco Us Inc. Improving fungal gene expression using insulator dna sequences
BR112014016587A2 (pt) 2012-01-05 2020-10-27 Novartis Ag células fúngicas filamentosas deficientes de protease e métodos de uso das mesmas
CN104781385A (zh) 2012-09-20 2015-07-15 丹尼斯科美国公司 用于对细胞培养物控释培养组分的微量滴定板
MX2015005427A (es) 2012-10-31 2015-08-05 Danisco Inc Beta-glucosidasa de magnaporthe grisea.
EP2914719A1 (en) 2012-10-31 2015-09-09 Danisco US Inc. Compositions and methods of use
WO2014070844A1 (en) 2012-10-31 2014-05-08 Danisco Us Inc. Beta-glucosidase from neurospora crassa
CN104870643A (zh) 2012-12-12 2015-08-26 丹尼斯科美国公司 纤维二糖水解酶的变体
EP3290515A1 (en) 2012-12-12 2018-03-07 Danisco US Inc. Variants of cellobiohydrolases
EP2931886A1 (en) 2012-12-12 2015-10-21 Danisco US Inc. Variants of cellobiohydrolases
BR112015013646A2 (pt) 2012-12-12 2017-11-14 Danisco Us Inc variante isolada de uma enzima celobio-hidrolase (cbh) parental, polinucleotídeo isolado, vetor, célula hospedeira, composição detergente, aditivo alimentar, método para hidrolisar um substrato celulósico, sobrenadante de cultura celular, métodos de produção de um polipeptídeo cbh variante e sobrenadante de cultura celular
CN104870642A (zh) 2012-12-12 2015-08-26 丹尼斯科美国公司 纤维二糖水解酶的变体
WO2015004241A2 (en) * 2013-07-10 2015-01-15 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
CN105829530A (zh) 2013-12-04 2016-08-03 丹尼斯科美国公司 包含β-葡萄糖苷酶多肽的组合物及其使用方法
JP2017532967A (ja) 2014-10-27 2017-11-09 ダニスコ・ユーエス・インク ベータ−グルコシダーゼに関連する組成物および方法
CN107667171A (zh) 2014-12-16 2018-02-06 丹尼斯科美国公司 真菌基因组修饰系统及使用方法
WO2017177289A1 (en) 2016-04-15 2017-10-19 Vtt International Oy Process for conversion of biomass into fermentable sugars with integrated enzyme
CN106978360B (zh) * 2017-04-24 2020-08-04 上海交通大学 一株高产纤维素酶里氏木霉重组菌株及其应用
CN113106114B (zh) * 2020-01-13 2024-04-12 中国科学院分子植物科学卓越创新中心 调控里氏木霉蛋白表达效率的因子、调控方法及应用
JP7799621B2 (ja) * 2020-04-22 2026-01-15 ダニスコ・ユーエス・インク 糸状菌細胞におけるタンパク質産生の増強のための組成物及び方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007530065A (ja) 2004-04-02 2007-11-01 ディーエスエム アイピー アセッツ ビー．ブイ． 改善された相同的組換え効率を有する糸状菌変異体
WO2011151512A2 (en) 2010-06-04 2011-12-08 Teknologian Tutkimuskeskus Vtt Method for protein production in filamentous fungi
JP2014503185A (ja) 2010-10-20 2014-02-13 ダニスコ・ユーエス・インク 熱安定性トリコデルマ（ｔｒｉｃｈｏｄｅｒｍａ）セルラーゼ

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Aisha Ellahi et al.，The Chromatin and Transcriptional Landscape of Native Saccharomyces cerevisiae Telomeres and Subtelomeric Domains.，Genetics，2015年03月30日，Vol.200, No.2，p.505-521，doi: 10.1534/genetics.115.175711
Grant A. Bitter and Kevin M. Egan，Expression of interferon-gamma from hybrid yeast GPD promoters containing upstream regulatory sequences from GAL1-GAL10 intergenic region.，Gene，1988年09月30日，Vol.69, No.2，p.193-207，doi: 10.1016/0378-1119(88)90430-1
Kuo A. et al.，ORF Names=M419DRAFT_98455，UniProtKB/TrEMBL，version 16，2016年09月07日，https://www.uniprot.org/uniprot/A0A024SEE4.txt?version=16
M Cameron Limon et al.，The effects of disruption of phosphoglucose isomerase gene on carbon utilisation and cellulase production in Trichoderma reesei Rut-C30.，Microb. Cell Fact.，2011年05月24日，Vol.10，Article 40，doi: 10.1186/1475-2859-10-40

Also Published As

Publication number Publication date
WO2018067599A1 (en) 2018-04-12
KR102593668B1 (ko) 2023-10-24
US10876103B2 (en) 2020-12-29
US20190309276A1 (en) 2019-10-10
EP3523415A1 (en) 2019-08-14
JP7605902B2 (ja) 2024-12-24
CN109790510A (zh) 2019-05-21
CN109790510B (zh) 2024-04-12
JP2023109919A (ja) 2023-08-08
US20220145278A1 (en) 2022-05-12
JP2019528797A (ja) 2019-10-17
KR20190057088A (ko) 2019-05-27

Similar Documents

Publication Publication Date Title
JP7605902B2 (ja) 誘導基質の非存在下における糸状菌細胞内でのタンパク質の産生
CN102939382B (zh) 丝状真菌宿主菌株和dna构建体以及它们的使用方法
JP7648614B2 (ja) タンパク質生産性が増強された表現型を含む糸状菌株及びその方法
JP7799621B2 (ja) 糸状菌細胞におけるタンパク質産生の増強のための組成物及び方法
US20250320512A1 (en) Compositions and methods for enhanced protein production in fungal cells
US20240376511A1 (en) Compositions and methods for enhanced protein production in fungal cells
WO2021100631A1 (ja) 変異糸状菌、及びそれを用いたタンパク質の製造方法
JP2025537244A (ja) タンパク質生産性が増強された表現型を含む糸状菌株及びその方法
WO2025165968A1 (en) Conditional regulation of protein function in filamentous fungal cells
JP2026500088A (ja) 一貫したタンパク質を生成するための組換え真菌株及びその方法
HK1178937A (en) Filamentous fungal host strains and dna constructs, and methods of use thereof

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190604

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20190604

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20201002

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20201002

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20210716

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210720

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20211020

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20220111

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20220404

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220610

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20221004

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20230104

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20230104

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230215

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230209

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230303

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20230425

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20230523

R150 Certificate of patent or registration of utility model

Ref document number: 7285780

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250