CN109770081A - A kind of method of microbial flora fermentation pannage - Google Patents

Abstract

The present invention relates to pannage technical fields, more particularly to a kind of method of microbial flora fermentation pannage, this method is to carry out fermentation to raw materials such as dregs of beans, rice bran, sweet potato waste, vinasse, corn flour and Chinese herbal medicine powders using microbial flora to prepare pannage, the transformation efficiency of feed is improved through everfermentation, improve the quality of feed, improve the mouthfeel of feed, feed is set to become crisp soft, increase palatability, anti-nutritional factors and harmful microbe content in raw material are reduced simultaneously, reduces feeding cost.It is good that the present invention is prepared pannage palatability, convenient for digestion, can effectively facilitate live pig weight gain while reducing feeding fine fodder, reducing cost, improve meat quality, keep pork fresh and tender palatable；After pig feed is used, the immunity of pig can be improved, reduces the generation of disease, while appetite can be promoted, improved food-intake, improve the quality of pork.

Description

A kind of method of microbial flora fermentation pannage

Technical field

The present invention relates to pannage technical fields, and in particular to a kind of method of microbial flora fermentation pannage.

Background technique

As the improvement of people's living standards, higher and higher for the demand of green food, pollution-free food, pork and its The internal organs meat traditional as Chinese people is even more to be concerned.Currently, there are many common feedstuffs type of pig, it is divided by nutrition Protein feed, energy feed, roughage, green forage, 7 major class of ensilage, mineral feed and feed addictive.Although All kinds of feeds have respective distinctive effect, also require to the feed arrangement of the pig of various different cultivars, especially vegetalitas, Anti-nutritional factors in animality and mineral substance is unfavorable for the digestion and absorption of pig.Meanwhile in the production of pork pig, because of anxious function Nearly benefit causes excessive using antibiotic and abuse prohibited items, and the harmful substance residual in animal food may be exceeded, sternly Ghost image rings the product quality of pork, to influence the health of people.Therefore, for the different approaches of pork product, mode And degree, environment-friendly feed substitute is studied, replaces those to easily cause the feed or additive of pork pollution, to produce The safety-type pork product of high quality a, it has also become new trend of future in feed industry.

Biological fermentation feed refers under manual control condition, and microorganism is by itself metabolic activity, by vegetalitas, dynamic Anti-nutritional factors in physical property and mineral substance is decomposed or conversion, and generation can more be searched for food, digests, be absorbed by livestock and poultry and nonhazardous The feedstuff of effect.Applied Biotechnology is increasingly taken seriously in feed industry, because it can turn waste into wealth, sufficiently Using trade waste, feed resource abundant had not only been provided in this way, but also alleviate environmental pollution.Biological feedstuff contains by it The advantage of good high content of protein and bioactive substance abundant pushes feed to the situation for alleviating feedstuff anxiety The development of industry and animal husbandry will play huge impetus.

Roughage undergoes microbial fermentation and manufactured feed is referred to as fermented feed, and the roughage for fermentation includes bran Skin, rice bran, stalk, herbage, various cakes (dregs of rice), pomace, vinasse etc..Fermented feed is added to animal diets by a certain percentage In, efficiency of feed utilization can be improved, reduce feeding cost, fermented feed can also supplement animal intestinal tract profitable strain, inhibit to have Evil bacterium, maintenance intestinal microecology balance, establishes intestinal immunological barrier, improves animal immunizing power, reduce the generation of Animal diseases, institute The expense that Animal diseases can also be treated or prevented by reducing with fermented feed, further decreases aquaculture cost.The feeding in China Expect resource shortage, annual to need from feedstuffs such as a large amount of corns of external import, this largely hinders China poultry The sustainable development of animal husbandry.And it is very rich including, the processing of farm products by-product resource including peanut meal, rice bran etc. in China Richness, these processing of farm products by-products belong to the feedstuff of green non-pollution, using their fermenting and producings fermented feed more It is to have the characteristics that green, pollution-free, rich in protein, vitamin composition are complete, this helps somewhat to delay The status for solving current internal feed resource shortage saves grain to development using processing of farm products by-product resources making fermented feed Type aquaculture, raising aquaculture economic benefit and social benefit are of great significance.

Summary of the invention

The object of the present invention is to provide a kind of method of microbial flora fermentation pannage, this method utilizes microbial flora Fermentation is carried out to processing of farm products by-product and prepares pannage, efficiency of feed utilization can be improved, feeding cost is reduced, is prepared Pannage palatability is good, can improve the immunity of pig after pig feed is used convenient for digestion, reduce the generation of disease, while can promote Appetite improves food-intake.

To achieve the above object, the present invention adopts the following technical scheme:

A kind of method of microbial flora fermentation pannage, comprising the following steps:

(1) following raw material is taken according to parts by weight: 50~75 parts of dregs of beans；40~70 parts of rice bran；35~55 parts of sweet potato waste；Vinasse 20 ~40 parts；35~55 parts of corn flour；10~15 parts of molasses；7~10 parts of Chinese herbal medicine powder and 1~3 part of salt, it is spare；

(2) it is crushed after mixing dregs of beans, rice bran, sweet potato waste, vinasse and corn flour, molasses is added in obtained mixed material It is uniformly mixed with salt；Then the biodiversity content for adjusting mixed material is 40~50%, obtains fermentation materials；

(3) microbial flora is added in fermentation materials to be uniformly mixed, the additional amount of microbial flora is fermentation materials weight 1.0~1.5%, then fermentation materials are moved into fermenting cellar, are fermented 3~4 days at 30~40 DEG C；

(4) obtained fermentation material is uniformly mixed with Chinese herbal medicine powder, is fermented 12~24 hours, fermentation ends are granulated, at 45 DEG C After drying, pannage is obtained.

The method of above-mentioned microbial flora fermentation pannage, the molasses are remained after cane sugar factory crystallization of sucrose white sugar Remaining molasses after remaining molasses or beet, sweet Chinese sorghum pole sugaring crystallization white sugar, water is not added in molasses, and is placed on sealing In container.

The Chinese herbal medicine powder is to press the honeysuckle after drying, purslane, dayflower, mountain Cape jasmine, dried orange peel and bermudagrass herb It is obtained according to crushing after the mixing of any weight ratio.

In the step (3), microorganism is added in the method for above-mentioned microbial flora fermentation pannage in fermentation materials Flora is after mixing, moreover it is possible to EM bacterium further be added and ferment together after mixing, the dosage of EM bacterium is that microbial flora is used The 1/3 of amount.

The method of above-mentioned microbial flora fermentation pannage, the cultural method of microbial flora described in step (3) The following steps are included:

1. the rotted leaf and soil that weight is each 50% are extracted by the ditch that virgin forest is fallen leaves more on Dayaoshan, Guangxi high mountain, It seals up for safekeeping in the Brown Glass Brown glass bottles and jars only sterilized, brings back the interior preservation of refrigerator that laboratory is placed in 0~4 DEG C, obtain original flora；It is described Dayao Mountain high mountain virgin forest be weeds, coniferous forest, broad-leaf forest and shrub mixed forest, not by artificial pollution, also not Through herding；Guangxi Zhuang Autonomous Region Yao Autonomous County of Jinxiu shrine mountain is located at Guangxi Gold show county town southwest, the also known as mountain Sheng Tang, with 20 kilometers of county town linear distance, 45 kilometers of highway distance, 150 square kilometres of the gross area, 1979 meters of top height above sea level, containing original The rotted leaf and soil of flora are weeds, coniferous forest, broad-leaf forest and the filling from 1000 meters to 1500 meters of hill-side height above sea level on shrine mountain The brook bank of the wooden mixed forest zones obtains；

2. the rotted leaf containing original flora and soil are taken out a part, grind to obtain mud in desinfection chamber；

3. the mud containing original flora to be added to the double distilled water of 10 times of weight, it is uniformly mixed, is dispensed into several transparent glass Glass culture bottle stands 15~30 days under conditions of 25~35 DEG C indoors, muddy and smelly water culture bottle is discarded, by water energy Enough become as clear as crystal culture bottle, filters out the clear water of the inside, be put into Clear glass bottles and jars, then observe 15~30 days, take Obtaining still as clear as crystal, not smelly clear water is strain A, spare；

4. distilled water is added in vial, in parts by weight the fresh mucus inside 2~3% chitterlings of addition, the chicken fermented 2~5%, 2~3% plain chocolate of excrement and pig manure dry powder, 3~5% starch, 2~3% molasses, 2~5% soy meals, 2~5% fresh white dish leafs With 2~5% livestock meat powders, remaining is distilled water, is stirred evenly, and covers under conditions of 25~40 DEG C place 25~30 indoors It, makes it rot smelly, takes its smelly solution, spare；

5. 3. obtained strain A that is walked；The double distilled water dilution of 10 times of weight is added, is dispensed into the saturating of several 100 ml In bright glass culture bottle, each 100 ml culture bottle instills the smelly solution 5ml 4. walked, is uniformly mixed, adds ventilating cover, in room It is cultivated 15~30 days under conditions of interior 20~35 DEG C, observes the solution of transparent glass culture bottle, take that it is still as clear as crystal, does not send out Smelly culture bottle solution is strain B, spare；

6. the culture medium by microwave disinfection is added in strain B, and 25~35 DEG C of condition expands culture 15~30 indoors It, obtains microbial flora；The composition weight content of the culture medium is as follows:

Beef extract 1.0g, peptone 1.0g, molasses 1.0g, starch 1.0g, urea 1.0g, Sodium lysinate 0.5g, sodium glutamate 0.5g, vitamin B 0.5g, potassium dihydrogen phosphate 1.0g, sodium chloride 0.5g, magnesium chloride 0.5g, yeast extract 1.0g, distilled water 100mL, pH 7.2~7.5；The culture medium preparation method is: each raw material is placed in triangular flask, pH value is adjusted in heating and dissolution, At 121 DEG C after packing, high pressure sterilization is obtained under conditions of 20min.

Heretofore described Chinese herbal medicine powder is by honeysuckle, purslane, dayflower, mountain Cape jasmine, dried orange peel and bermudagrass herb group At, wherein honeysuckle is caprifoliaceae plant honeysuckle Lonicera japonica Thunb., lonicera hypoglauca miq Lonicera Hypoglauca Miq., Honeysuckle flower (hair calyx honeysuckle) Lonicera confusa DC. or hair style honeysuckle Lonicera The dry flower of dasystyla Rehd. or with the flower just opened.Modern study proves that honeysuckle contains chlorogenic acid, luteolin The pharmacological components such as glycosides cause a disease to the various pathogens such as hemolytic streptococcus, Staphylococcus aureus and the infection of the upper respiratory tract sick Poison etc. has stronger restraint, in addition can also strengthen immunity, Robust speaker feature, protect liver, antitumor, anti-inflammatory, antipyretic, hemostasis is (solidifying Blood), inhibit intestinal absorption cholesterol etc..Honeysuckle is cold in nature, sweet in flavor, enters lung, the heart, stomach meridian, has clearing heat and detoxicating, anti-inflammatory, qi-restoratives The effect for the treatment of wind, cures mainly disease under turgor, warm disease heating, the diseases such as heat toxin large carbuncle and tumour.Its for dizziness, thirst, Sweating and boring, enteritis, bacillary dysentery, morbilli, pneumonia, encephalitis, epidemic meningitis, acute mastitis, septicemia, ecphyaditis, skin infection, ulcer The illnesss such as furunculosis, erysipelas, parotitis, suppurative tonsillitis have certain curative effect.

Purslane (scientific name: Portulaca oleracea L.), with all herbal medicine.Major function: clearing heat and promoting diuresis, cool blood Removing toxic substances.For bacillary dysentery, acute gastroenteritis, acute appendicitis, mazoitis, hemorrhoid hemorrhage, leukorrhea；It is swollen that furunculosis is controlled in external application Poison, eczema, shingles zoster.Purslane vitamin A sample substance rich in, therefore the physiological function of epithelial cell can be promoted to tend to Normally, and the healing of ulcer can be promoted.Purslane has inhibiting effect to Escherichia coli, shigella dysenteriae, typhoid bacillus；To common Pathogenic dermatophyte also has inhibiting effect.Purslane two oxyethylamine rich in, malic acid, glucose, calcium, phosphorus, iron with And the nutriments such as vitamin E, carrotene, vitamin B, vitamin C.Medicinal situation: herb controls dysentery, enteritis, diarrhea, pharynx Laryngalgia, toothache, nameless sores or boils " osmanthus medicine volume ".

Dayflower, Chinese medicine name are the drying overground part of commelianaceae plant dayflower Commelina communis L. Point.Sweet in flavor, slight bitter, it is cold in nature.Energy heat-clearing, removing toxic substances, diuresis.For swelling diuretic, clearing heat and detoxicating good medicine, furthermore to sty, pharynx Inflammation, tonsillitis, cervical erosion, abdomen snake bite have good efficacy.Herb (joliolide) containing left-handed~loliolide, nothing It controls terpene (friedelin), β~sitosterol (β~sitosterol).Right~hydroxyl cinnamic acid (p~hydroxy~cinnamic Acid), Daucosterol (dancosterol) and D~mannitol (mannose) and N-triacontanol (n~triacontanol). Major function: row water, heat-clearing, cool blood, removing toxic substances；It harnesses the river swollen, tinea pedis, difficult urination, flu, erysipelas, parotitis, icterohepatitis, Hot dysentery, malaria, nosebleed epistaxis, hematuria, metrorrhagia, leukorrhea, abscess of throat, ulcer boils.

Mountain Cape jasmine (Gardenia jasminoides) also known as Mu Dan, fresh branch, water cross branch, stand flower, branch flower, mountain Cape jasmine flower Deng for the fruit of dicotyledon medicine madder wort mountain Cape jasmine.Leaf, flower, fruit and the root of mountain Cape jasmine can be used as medicine, have heat-clearing, The effect of purging intense heat, removing toxic substances, cool blood.The effect of mountain Cape jasmine and effect: purging intense heat relieving restlessness；Clearing heat and promoting diuresis；Removing pattogenic heat from the blood and toxic material from the body.Cure mainly the pyreticosis heart Tired, irascibility hot eyes, damp and hot yellow infantile malnutrition due to digestive disturbances or intestinalparasites, stranguria, bloody flux hematuria, aphthae, sore swollen toxin, sprains swelling and pain at headache.

Dried orange peel is the drying and ripening fruit of rutaceae orange Citrus reticulata Blanco and its variety Skin.[nature and flavor]: warm-natured, acrid flavour, bitter.[channel tropism]: enter the spleen channel, stomach meridian, lung channel.[attending effectiveness]: regulating qi-flowing for strengthening spleen, it is dry in tune It is wet, resolving sputum.Cure mainly the abdominal fullness and distention or pain, indigestion of the taste stagnation of the circulation of vital energy.Abdominal distension uncomfortable in chest, anorexia and loose stool in wet turbid resistance.Phlegm The wet cough and asthma for stopping up lung.For chest gastral cavity turgor, deficiency of food is vomited and diarrhoea, coughing with a lot of sputum.Dried orange peel has three categories effect, trembles with fear in the heart first is that leading Heresy, two broken stagnant gas, three Energy benefit tastes.In this three big effect, main function is row QI of the spleen and stomach.The main transmitting and distributing the fluids of taste, therefore taste Gas row can then dry, invigorating the spleen, resolving sputum, it is pungent to be amusing therefore again it can be said that dried orange peel temperature can support spleen, it is bitter can invigorating the spleen.

Bermudagrass herb is the herb of grass family Cynodon plant Bermuda grass Cynodon dactylon (L.) Pers.. Nature and flavor sweet and neutral.Bermudagrass herb can wind-dispelling, it is active, it is antipyretic, hemostasis, myogenic.Control rheumatism scabies numbness muscular constricture, hemiplegia, over-strained hemoptysis, Bruise, knife wound, ulcer on the shank.Reducing fever and causing diuresis, dissipating stasis and stanching bleeding, relaxing tendons and activating collaterals.For the infection of the upper respiratory tract, hepatitis, dysentery, the urinary tract sense Dye, nosebleed epistaxis, hemoptysis, spitting blood, hematochezia, tinea pedis oedema, treating rheumatic ostealgia, nettle rash, hemiplegia, numbness in hands and feet, traumatic injury；Outside With traumatic hemorrhage is controlled, fracture, sore carbuncle, ulcus cruris.Popularity impression emit, catch a cold, coughing, hepatitis, dysentery, pain in the loins, urethra knot Stone, newborn pain, consumptive disease wound, traumatic injury, burn and scald, snake bite, serious case of furuncle, have very high medical value.

The invention has the benefit that

1, the fermentative microflora used in the present invention is to breed to obtain by screening and scientific method from the Nature, is had superpower Breeding and adaptability, the present invention using microbial flora to the raw materials such as dregs of beans, rice bran, sweet potato waste, vinasse, corn flour carry out Fermentation prepares pannage, the nonabsorbable polymeric carbohydrate of animal can be converted to the absorbable low molecule carbon water utilized Compound, microbial bacteria can also largely absorb organic nitrogen, the inorganic nitrogen that animal is difficult to be utilized and be allowed to be converted to animal convenient for utilizing A variety of bacterium proteins, the fermentative microflora is during feed fermentation, moreover it is possible to inhibit the growth of pathogen and spoilage organisms, thus Feed quality is improved, the transformation efficiency of feed is improved, improves the mouthfeel of feed, feed is made to become crisp soft, increases palatability, Anti-nutritional factors and harmful microbe content in raw material are reduced simultaneously, reduces feeding cost, reduces pig illness incidence, are promoted Pig growth.

2, in raw material used in the method for microbial flora of the present invention fermentation pannage also containing by honeysuckle, purslane, The medicinal herb components that dayflower, mountain Cape jasmine, dried orange peel and bermudagrass herb form, medicinal herb components, which are added, in fermented feed can be improved It is horizontal to reduce live pig Blood Cholesterol for the ability of live pig resisting stress and resistance to disease；There is reinforcing spleen to promote digestion, detoxify desinsection Effect, the generation of the diseases such as flu, asthma, the transmissible gastroenteritis of pig can be substantially reduced, it is antibacterial, antiviral, enhance it is disease-resistant Power, the effective component in Chinese herbal medicine after everfermentation enable to pig to be easier to absorb, are remarkably improved animal immune ability, mention High feed conversion rate.

3, it is good that pannage palatability is prepared in the present invention, convenient for digestion, can reduce feeding fine fodder, reduce cost Meanwhile live pig weight gain is effectively facilitated, improve meat quality, keeps pork fresh and tender palatable；After pig feed is used, the immunity of pig can be improved, The generation of disease is reduced, while appetite can be promoted, food-intake is improved, improves the quality of pork.

Specific embodiment

Embodiment 1

A kind of method of microbial flora fermentation pannage, comprising the following steps:

(1) following raw material is taken according to parts by weight: 50 parts of dregs of beans；40 parts of rice bran；35 parts of sweet potato waste；20 parts of vinasse；Corn flour 35 Part；10 parts of molasses；7 parts of Chinese herbal medicine powder and 1 part of salt, it is spare；The molasses are remained after cane sugar factory crystallization of sucrose white sugar Remaining molasses after remaining molasses or beet, sweet Chinese sorghum pole sugaring crystallization white sugar, water is not added in molasses, and is placed on sealing In container；The Chinese herbal medicine powder is to press the honeysuckle after drying, purslane, dayflower, mountain Cape jasmine, dried orange peel and bermudagrass herb It is obtained according to crushing after the mixing of any weight ratio；

(2) it is crushed after mixing dregs of beans, rice bran, sweet potato waste, vinasse and corn flour, molasses is added in obtained mixed material It is uniformly mixed with salt；Then the biodiversity content for adjusting mixed material is 40%, obtains fermentation materials；

(3) microbial flora is added in fermentation materials to be uniformly mixed, the additional amount of microbial flora is fermentation materials weight 1.0%, then fermentation materials are moved into fermenting cellar, are fermented 4 days at 30 DEG C；

(4) obtained fermentation material is uniformly mixed with Chinese herbal medicine powder, is fermented 12 hours, fermentation ends are granulated, dry at 45 DEG C Afterwards, pannage is obtained.

The cultural method of microbial flora described in above-mentioned steps (3) the following steps are included:

1. the rotted leaf and soil that weight is each 50% are extracted by the ditch that virgin forest is fallen leaves more on Dayaoshan, Guangxi high mountain, It seals up for safekeeping in the Brown Glass Brown glass bottles and jars only sterilized, brings back the interior preservation of refrigerator that laboratory is placed in 0~4 DEG C, obtain original flora；It is described Dayao Mountain high mountain virgin forest be weeds, coniferous forest, broad-leaf forest and shrub mixed forest, not by artificial pollution, also not Through herding；Guangxi Zhuang Autonomous Region Yao Autonomous County of Jinxiu shrine mountain is located at Guangxi Gold show county town southwest, the also known as mountain Sheng Tang, with 20 kilometers of county town linear distance, 45 kilometers of highway distance, 150 square kilometres of the gross area, 1979 meters of top height above sea level, containing original The rotted leaf and soil of flora are weeds, coniferous forest, broad-leaf forest and the filling from 1000 meters to 1500 meters of hill-side height above sea level on shrine mountain The brook bank of the wooden mixed forest zones obtains；

2. the rotted leaf containing original flora and soil are taken out a part, grind to obtain mud in desinfection chamber；

3. the mud containing original flora to be added to the double distilled water of 10 times of weight, it is uniformly mixed, is dispensed into several transparent glass Glass culture bottle stands 30 days under conditions of 25 DEG C indoors, discards muddy and smelly water culture bottle, water energy, which is reached, becomes clear Clear transparent culture bottle filters out the clear water of the inside, is put into Clear glass bottles and jars, then observe 15 days, obtains still limpid Bright, not smelly clear water is strain A, spare；

4. distilled water is added in vial, the fresh mucus inside 2% chitterlings, the chicken manure to ferment are added in parts by weight With pig manure dry powder 5%, 2% plain chocolate, 5% starch, 2% molasses, 5% soy meal, 2% fresh white dish leaf and 5% livestock meat powder, remaining It for distilled water, stirs evenly, covers under conditions of 25 DEG C place 30 days indoors, so that it is rotted smelly, take its smelly molten Liquid, it is spare；

5. 3. obtained strain A that is walked；The double distilled water dilution of 10 times of weight is added, is dispensed into the saturating of several 100 ml In bright glass culture bottle, each 100 ml culture bottle instills the smelly solution 5ml 4. walked, is uniformly mixed, adds ventilating cover, in room It is cultivated 30 days under conditions of interior 20 DEG C, observes the solution of transparent glass culture bottle, the culture for taking it still as clear as crystal, not smelly Bottle solution is strain B, spare；

6. the culture medium by microwave disinfection is added in strain B, and 25 DEG C of condition expands culture 30 days indoors, obtain micro- Biological flora；The composition weight content of the culture medium is as follows:

Beef extract 1.0g, peptone 1.0g, molasses 1.0g, starch 1.0g, urea 1.0g, Sodium lysinate 0.5g, sodium glutamate 0.5g, vitamin B 0.5g, potassium dihydrogen phosphate 1.0g, sodium chloride 0.5g, magnesium chloride 0.5g, yeast extract 1.0g, distilled water 100mL, pH 7.2~7.5；The culture medium preparation method is: each raw material is placed in triangular flask, pH value is adjusted in heating and dissolution, At 121 DEG C after packing, high pressure sterilization is obtained under conditions of 20min.

Embodiment 2

A kind of method of microbial flora fermentation pannage, comprising the following steps:

(1) following raw material is taken according to parts by weight: 65 parts of dregs of beans；55 parts of rice bran；45 parts of sweet potato waste；30 parts of vinasse；Corn flour 50 Part；14 parts of molasses；8 parts of Chinese herbal medicine powder and 2 parts of salt, it is spare；The molasses are remained after cane sugar factory crystallization of sucrose white sugar Remaining molasses after remaining molasses or beet, sweet Chinese sorghum pole sugaring crystallization white sugar, water is not added in molasses, and is placed on sealing In container；The Chinese herbal medicine powder is to press the honeysuckle after drying, purslane, dayflower, mountain Cape jasmine, dried orange peel and bermudagrass herb It is obtained according to crushing after the mixing of any weight ratio；

(2) it is crushed after mixing dregs of beans, rice bran, sweet potato waste, vinasse and corn flour, molasses is added in obtained mixed material It is uniformly mixed with salt；Then the biodiversity content for adjusting mixed material is 45%, obtains fermentation materials；

(3) microbial flora is added in fermentation materials to be uniformly mixed, the additional amount of microbial flora is fermentation materials weight 1.3%, then fermentation materials are moved into fermenting cellar, are fermented 3.5 days at 35 DEG C；

(4) obtained fermentation material is uniformly mixed with Chinese herbal medicine powder, is fermented 20 hours, fermentation ends are granulated, dry at 45 DEG C Afterwards, pannage is obtained.

The cultural method of microbial flora described in above-mentioned steps (3) the following steps are included:

1. the rotted leaf and soil that weight is each 50% are extracted by the ditch that virgin forest is fallen leaves more on Dayaoshan, Guangxi high mountain, It seals up for safekeeping in the Brown Glass Brown glass bottles and jars only sterilized, brings back the interior preservation of refrigerator that laboratory is placed in 0~4 DEG C, obtain original flora；It is described Dayao Mountain high mountain virgin forest be weeds, coniferous forest, broad-leaf forest and shrub mixed forest, not by artificial pollution, also not Through herding；Guangxi Zhuang Autonomous Region Yao Autonomous County of Jinxiu shrine mountain is located at Guangxi Gold show county town southwest, the also known as mountain Sheng Tang, with 20 kilometers of county town linear distance, 45 kilometers of highway distance, 150 square kilometres of the gross area, 1979 meters of top height above sea level, containing original The rotted leaf and soil of flora are weeds, coniferous forest, broad-leaf forest and the filling from 1000 meters to 1500 meters of hill-side height above sea level on shrine mountain The brook bank of the wooden mixed forest zones obtains；

2. the rotted leaf containing original flora and soil are taken out a part, grind to obtain mud in desinfection chamber；

3. the mud containing original flora to be added to the double distilled water of 10 times of weight, it is uniformly mixed, is dispensed into several transparent glass Glass culture bottle stands 25 days under conditions of 30 DEG C indoors, discards muddy and smelly water culture bottle, water energy, which is reached, becomes clear Clear transparent culture bottle filters out the clear water of the inside, is put into Clear glass bottles and jars, then observe 25 days, obtains still limpid Bright, not smelly clear water is strain A, spare；

4. distilled water is added in vial, the fresh mucus inside 3% chitterlings, the chicken manure to ferment are added in parts by weight With pig manure dry powder 2%, 3% plain chocolate, 3% starch, 3% molasses, 2% soy meal, 5% fresh white dish leaf and 2% livestock meat powder, remaining It for distilled water, stirs evenly, covers under conditions of 30 DEG C place 28 days indoors, so that it is rotted smelly, take its smelly molten Liquid, it is spare；

5. 3. obtained strain A that is walked；The double distilled water dilution of 10 times of weight is added, is dispensed into the saturating of several 100 ml In bright glass culture bottle, each 100 ml culture bottle instills the smelly solution 5ml 4. walked, is uniformly mixed, adds ventilating cover, in room It is cultivated 25 days under conditions of interior 30 DEG C, observes the solution of transparent glass culture bottle, the culture for taking it still as clear as crystal, not smelly Bottle solution is strain B, spare；

6. the culture medium by microwave disinfection is added in strain B, and 30 DEG C of condition expands culture 25 days indoors, obtain micro- Biological flora；The composition weight content of the culture medium is as follows:

Beef extract 1.0g, peptone 1.0g, molasses 1.0g, starch 1.0g, urea 1.0g, Sodium lysinate 0.5g, sodium glutamate 0.5g, vitamin B 0.5g, potassium dihydrogen phosphate 1.0g, sodium chloride 0.5g, magnesium chloride 0.5g, yeast extract 1.0g, distilled water 100mL, pH 7.2~7.5；The culture medium preparation method is: each raw material is placed in triangular flask, pH value is adjusted in heating and dissolution, At 121 DEG C after packing, high pressure sterilization is obtained under conditions of 20min.

Embodiment 3

A kind of method of microbial flora fermentation pannage, comprising the following steps:

(1) following raw material is taken according to parts by weight: 75 parts of dregs of beans；70 parts of rice bran；55 parts of sweet potato waste；40 parts of vinasse；Corn flour 55 Part；15 parts of molasses；10 parts of Chinese herbal medicine powder and 3 parts of salt, it is spare；The molasses are remained after cane sugar factory crystallization of sucrose white sugar Remaining molasses after remaining molasses or beet, sweet Chinese sorghum pole sugaring crystallization white sugar, water is not added in molasses, and is placed on sealing In container；The Chinese herbal medicine powder is to press the honeysuckle after drying, purslane, dayflower, mountain Cape jasmine, dried orange peel and bermudagrass herb It is obtained according to crushing after the mixing of any weight ratio；

(2) it is crushed after mixing dregs of beans, rice bran, sweet potato waste, vinasse and corn flour, molasses is added in obtained mixed material It is uniformly mixed with salt；Then the biodiversity content for adjusting mixed material is 50%, obtains fermentation materials；

(3) microbial flora is added in fermentation materials to be uniformly mixed, the additional amount of microbial flora is fermentation materials weight 1.5%, then fermentation materials are moved into fermenting cellar, are fermented 3 days at 40 DEG C；

(4) obtained fermentation material is uniformly mixed with Chinese herbal medicine powder, is fermented 24 hours, fermentation ends are granulated, dry at 45 DEG C Afterwards, pannage is obtained.

The cultural method of microbial flora described in above-mentioned steps (3) the following steps are included:

1. the rotted leaf and soil that weight is each 50% are extracted by the ditch that virgin forest is fallen leaves more on Dayaoshan, Guangxi high mountain, It seals up for safekeeping in the Brown Glass Brown glass bottles and jars only sterilized, brings back the interior preservation of refrigerator that laboratory is placed in 0~4 DEG C, obtain original flora；It is described Dayao Mountain high mountain virgin forest be weeds, coniferous forest, broad-leaf forest and shrub mixed forest, not by artificial pollution, also not Through herding；Guangxi Zhuang Autonomous Region Yao Autonomous County of Jinxiu shrine mountain is located at Guangxi Gold show county town southwest, the also known as mountain Sheng Tang, with 20 kilometers of county town linear distance, 45 kilometers of highway distance, 150 square kilometres of the gross area, 1979 meters of top height above sea level, containing original The rotted leaf and soil of flora are weeds, coniferous forest, broad-leaf forest and the filling from 1000 meters to 1500 meters of hill-side height above sea level on shrine mountain The brook bank of the wooden mixed forest zones obtains；

2. the rotted leaf containing original flora and soil are taken out a part, grind to obtain mud in desinfection chamber；

3. the mud containing original flora to be added to the double distilled water of 10 times of weight, it is uniformly mixed, is dispensed into several transparent glass Glass culture bottle stands 15 days under conditions of 35 DEG C indoors, discards muddy and smelly water culture bottle, water energy, which is reached, becomes clear Clear transparent culture bottle filters out the clear water of the inside, is put into Clear glass bottles and jars, then observe 30 days, obtains still limpid Bright, not smelly clear water is strain A, spare；

4. distilled water is added in vial, the fresh mucus inside 2.5% chitterlings, the chicken fermented are added in parts by weight Excrement and pig manure dry powder 3%, 2.5% plain chocolate, 4% starch, 2.5% molasses, 3% soy meal, 4% fresh white dish leaf and 4% livestock meat are broken End, remaining is distilled water, is stirred evenly, and covers under conditions of 40 DEG C place 25 days indoors, so that it is rotted smelly, take it Smelly solution, it is spare；

5. 3. obtained strain A that is walked；The double distilled water dilution of 10 times of weight is added, is dispensed into the saturating of several 100 ml In bright glass culture bottle, each 100 ml culture bottle instills the smelly solution 5ml 4. walked, is uniformly mixed, adds ventilating cover, in room It is cultivated 15 days under conditions of interior 35 DEG C, observes the solution of transparent glass culture bottle, the culture for taking it still as clear as crystal, not smelly Bottle solution is strain B, spare；

6. the culture medium by microwave disinfection is added in strain B, and 35 DEG C of condition expands culture 15 days indoors, obtain micro- Biological flora；The composition weight content of the culture medium is as follows:

Beef extract 1.0g, peptone 1.0g, molasses 1.0g, starch 1.0g, urea 1.0g, Sodium lysinate 0.5g, sodium glutamate 0.5g, vitamin B 0.5g, potassium dihydrogen phosphate 1.0g, sodium chloride 0.5g, magnesium chloride 0.5g, yeast extract 1.0g, distilled water 100mL, pH 7.2~7.5；The culture medium preparation method is: each raw material is placed in triangular flask, pH value is adjusted in heating and dissolution, At 121 DEG C after packing, high pressure sterilization is obtained under conditions of 20min.

Embodiment 4

A kind of method of microbial flora fermentation pannage, comprising the following steps:

(1) following raw material is taken according to parts by weight: 70 parts of dregs of beans；60 parts of rice bran；45 parts of sweet potato waste；30 parts of vinasse；Corn flour 40 Part；14 parts of molasses；9 parts of Chinese herbal medicine powder and 2 parts of salt, it is spare；The molasses are remained after cane sugar factory crystallization of sucrose white sugar Remaining molasses after remaining molasses or beet, sweet Chinese sorghum pole sugaring crystallization white sugar, water is not added in molasses, and is placed on sealing In container；The Chinese herbal medicine powder is to press the honeysuckle after drying, purslane, dayflower, mountain Cape jasmine, dried orange peel and bermudagrass herb It is obtained according to crushing after the mixing of any weight ratio；

(2) it is crushed after mixing dregs of beans, rice bran, sweet potato waste, vinasse and corn flour, molasses is added in obtained mixed material It is uniformly mixed with salt；Then the biodiversity content for adjusting mixed material is 48%, obtains fermentation materials；

(3) microbial flora is added in fermentation materials and EM bacterium is uniformly mixed, the additional amount of microbial flora is fermentation materials The dosage of 1.4%, the EM bacterium of weight is the 1/3 of microbial flora dosage, then moves into fermentation materials in fermenting cellar, in 30~ It ferments 3 days at 40 DEG C；The cultural method of the microbial flora is consistent in embodiment 2；

(4) obtained fermentation material is uniformly mixed with Chinese herbal medicine powder, is fermented 24 hours, fermentation ends are granulated, dry at 45 DEG C Afterwards, pannage is obtained.

Feeding experiment:

(1) test material

Test selection parity, the substantially similar pig 600 of age in days and weight, be randomly divided into 1., 2., 3. three groups, every group 200, Male and female is fifty-fifty.

1. group feeds basic mixed fodder (corn 65%+ dregs of beans 20+ wheat bran 15%),

2. group feeds pannage produced by the present invention；

3. group, the feed that feeding basis mixed fodder 50%+ pannage produced by the present invention 50% is mixed to get.

(2) test method

Test pig is raised using group, and day feeds 2 times, and free water, other management carry out according to a conventional method, and feed management method is identical. Three groups measure original body mass respectively, and experiment terminates measurement latter stage weight, records feed consumption, disease incidence, and calculating averagely increases day by day Weight and feedstuff-meat ratio, experimental period 32 days.

(3) test result is as shown in table 1 below:

It can be seen in table 1 that good gaining effect can be played to pig using the pannage that the present invention is prepared, and reduce Feed-weight ratio, and when feeding the feed that basis mixed fodder 50%+ pannage produced by the present invention 50% is mixed to get, it remains to reach The effect of weight gain；By comparing, the pig speed of weight increment for eating feed of the present invention is very fast, and sick rate is low, and the spiritual expression shape of pig State is fine, likes eating feed produced by the present invention.

Claims (7)

1. a kind of method of microbial flora fermentation pannage, which comprises the following steps:

(1) following raw material is taken according to parts by weight: 50~75 parts of dregs of beans；40~70 parts of rice bran；35~55 parts of sweet potato waste；Vinasse 20 ~40 parts；35~55 parts of corn flour；10~15 parts of molasses；7~10 parts of Chinese herbal medicine powder and 1~3 part of salt, it is spare；

(2) it is crushed after mixing dregs of beans, rice bran, sweet potato waste, vinasse and corn flour, molasses is added in obtained mixed material It is uniformly mixed with salt；Then the biodiversity content for adjusting mixed material is 40~50%, obtains fermentation materials；

(3) microbial flora is added in fermentation materials to be uniformly mixed, the additional amount of microbial flora is fermentation materials weight 1.0~1.5%, then fermentation materials are moved into fermenting cellar, are fermented 3~4 days at 30~40 DEG C；

(4) obtained fermentation material is uniformly mixed with Chinese herbal medicine powder, is fermented 12~24 hours, fermentation ends are granulated, at 45 DEG C After drying, pannage is obtained.

2. the method for microbial flora fermentation pannage according to claim 1, which is characterized in that the molasses are sweet Remaining molasses or beet, sweet Chinese sorghum pole sugaring crystallize remaining molasses after white sugar, sugar after cane mill's crystallization of sucrose white sugar Water is not added in honey, and places in a sealed container.

3. the method for microbial flora fermentation pannage according to claim 1, which is characterized in that the Chinese herbal medicine powder It is by the honeysuckle after drying, purslane, dayflower, mountain Cape jasmine, dried orange peel and bermudagrass herb, according to powder after the mixing of any weight ratio It is broken to obtain.

4. the method for microbial flora fermentation pannage according to claim 1, which is characterized in that in the step (3), Microbial flora is added after mixing in fermentation materials, moreover it is possible to EM bacterium further be added and ferment together after mixing.

5. the method for microbial flora fermentation pannage according to claim 1, which is characterized in that described in step (3) Microbial flora cultural method the following steps are included:

1. the rotted leaf and soil that weight is each 50% are extracted by the ditch that virgin forest is fallen leaves more on Dayaoshan, Guangxi high mountain, It seals up for safekeeping in the Brown Glass Brown glass bottles and jars only sterilized, brings back the interior preservation of refrigerator that laboratory is placed in 0~4 DEG C, obtain original flora；

2. the rotted leaf containing original flora and soil are taken out a part, grind to obtain mud in desinfection chamber；

3. the mud containing original flora to be added to the double distilled water of 10 times of weight, it is uniformly mixed, is dispensed into several transparent glass Glass culture bottle stands 15~30 days under conditions of 25~35 DEG C indoors, muddy and smelly water culture bottle is discarded, by water energy Enough become as clear as crystal culture bottle, filters out the clear water of the inside, be put into Clear glass bottles and jars, then observe 15~30 days, take Obtaining still as clear as crystal, not smelly clear water is strain A, spare；

4. distilled water is added in vial, in parts by weight the fresh mucus inside 2~3% chitterlings of addition, the chicken fermented 2~5%, 2~3% plain chocolate of excrement and pig manure dry powder, 3~5% starch, 2~3% molasses, 2~5% soy meals, 2~5% fresh white dish leafs With 2~5% livestock meat powders, remaining is distilled water, is stirred evenly, and covers under conditions of 25~40 DEG C place 25~30 indoors It, makes it rot smelly, takes its smelly solution, spare；

5. 3. obtained strain A that is walked；The double distilled water dilution of 10 times of weight is added, is dispensed into the saturating of several 100 ml In bright glass culture bottle, each 100 ml culture bottle instills the smelly solution 5ml 4. walked, is uniformly mixed, adds ventilating cover, in room It is cultivated 15~30 days under conditions of interior 20~35 DEG C, observes the solution of transparent glass culture bottle, take that it is still as clear as crystal, does not send out Smelly culture bottle solution is strain B, spare；

6. the culture medium by microwave disinfection is added in strain B, and 25~35 DEG C of condition expands culture 15~30 indoors It, obtains microbial flora.

6. the cultural method of microbial flora according to claim 5, it is characterised in that: the Dayao Mountain high mountain is original Forest be weeds, coniferous forest, broad-leaf forest and shrub mixed forest, not by artificial pollution, also without herding；Zhuang nationality in Guangxi Autonomous region, shrine mountain, Yao Autonomous County of Jinxiu is located at Guangxi Gold show county town southwest, the also known as mountain Sheng Tang, public with county town linear distance 20 In, 45 kilometers of highway distance, 150 square kilometres of the gross area, 1979 meters of top height above sea level, rotted leaf and soil containing original flora Be the weeds from 1000 meters to 1500 meters of hill-side height above sea level on shrine mountain, coniferous forest, broad-leaf forest and shrub mixed forest zones brook Side obtains.

7. the cultural method of microbial flora according to claim 5, it is characterised in that: culture medium 6. walked Composition weight content it is as follows:

Beef extract 1.0g, peptone 1.0g, molasses 1.0g, starch 1.0g, urea 1.0g, Sodium lysinate 0.5g, sodium glutamate 0.5g, vitamin B 0.5g, potassium dihydrogen phosphate 1.0g, sodium chloride 0.5g, magnesium chloride 0.5g, yeast extract 1.0g, distilled water 100mL, pH 7.2~7.5；The culture medium preparation method is: each raw material is placed in triangular flask, pH value is adjusted in heating and dissolution, At 121 DEG C after packing, high pressure sterilization is obtained under conditions of 20min.